High costs of infection: Alphavirus infection reduces digestive function and bone and feather growth in nestling house sparrows (Passer domesticus)

Increasingly, ecoimmunology studies aim to use relevant pathogen exposure to examine the impacts of infection on physiological processes in wild animals. Alphaviruses are arthropod-borne, single-stranded RNA (ssRNA) viruses (“arboviruses”) responsible for millions of cases of human illnesses each year. Buggy Creek virus (BCRV) is a unique alphavirus that is transmitted by a cimicid insect, the swallow bug, and is amplified in two avian species: the house sparrow (Passer domesticus) and the cliff swallow (Petrochelidon pyrrhonota). BCRV, like many alphaviruses, exhibits age-dependent susceptibility where the young are most susceptible to developing disease and exhibit a high mortality rate. However, alphavirus disease etiology in nestling birds is unknown. In this study, we infected nestling house sparrows with Buggy Creek virus and measured virological, pathological, growth, and digestive parameters following infection. Buggy Creek virus caused severe encephalitis in all infected nestlings, and the peak viral concentration in brain tissue was over 34 times greater than any other tissue. Growth, tissue development, and digestive function were all significantly impaired during BCRV infection. However, based on histopathological analysis performed, this impairment does not appear to be the result of direct tissue damage by the virus, but likely caused by encephalitis and neuronal invasion and impairment of the central nervous system. This is the first study to examine the course of alphavirus diseases in nestling birds and these results will improve our understanding of age-dependent infections of alphaviruses in vertebrate hosts.


Introduction
Interest in the interplay among infectious diseases, their associated immune responses, and life history traits in wild organisms has dramatically increased over the past two decades [1,2,3]. a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 The incidence of naturally-acquired BCRV infection levels are low in nestling cliff swallows (~2%) but higher in nestling house sparrows (20%) [18]. There is no record of BCRV-induced disease in wild-caught nestling or adult cliff swallows or adult house sparrows. Experimental inoculation of adult house sparrows with BCRV causes minimal viremia, no recorded mortality, and significant upregulation of pattern recognition receptors and cytokines associated with antiviral and pro-inflammatory responses [3]. Severe BCRV-induced neurological disease and high levels of viral load have been recorded in wild-caught house sparrow nestlings [18] Furthermore, house sparrow nestlings have been shown to have immature innate and adaptive immune systems [37,38], which could lead to inefficient immune response and regulation, and increased BCRV disease susceptibility. BCRV infection in nestling house sparrows appears to have broad ecological consequences. Among field-sampled nestling house sparrows tested at 4-6 days of age, only 12.8% that were positive for BCRV ultimately fledged, versus 50.5% of those that were negative [39]. However, the mechanism by which BCRV infection impairs fledging success is unknown.
The nestling period of birds is energetically expensive due to the rapid anatomical growth and maturation that occurs during this time. Inflammatory responses to infection can be costly to birds in terms of energy, pathological damage, decreased appetite and impaired growth [40,41]. Impaired nestling growth has been shown to delay fledging [42,43,44], and decrease predicted survival of post-fledging birds in the wild [45,46]. Therefore, the impacts of infectious diseases may influence the success of the nestling and fledgling periods of birds. Nestling development is closely linked to digestive function, and the time course for development of the digestive system and body size and composition in altricial nestlings has been documented under normal conditions and in conditions of altered food availability and quality [47,48]. However, to our knowledge, no studies exist which have examined the impacts of an active pathogen infection on the development and physiology of nestling birds. In this study, we infected house sparrow nestlings with BCRV, quantified infection severity through serological methods and pathology, and measured the impacts of infection on digestive function, bone growth, and feather growth.

Ethics statement
All procedures involving animals were approved by the Creighton University Institutional Animal Care and Use Committee under protocol 0932. Animals were euthanized by CO 2 overdose, followed by cervical dislocation. House sparrows are a non-native species, and federal and state permits are not required for collection of this species. House sparrows were collected from private land, and landowner permission to collect the nestlings was granted prior to start of the experiment.

Study organisms
Three or four day-old house sparrow (Passer domesticus) nestlings were collected May-June, 2012, from nest boxes in the Council Bluffs, Iowa, and Omaha, Nebraska area. Nestlings were transported to the laboratory and housed in a plastic beaker lined with a waterproof mat in a temperature-controlled room (35˚C) and 15-hour illumination (equivalent to the natural photoperiod during May-June in our study area).
Nestlings were weighed before the first feeding at 06:30h and after the last feeding at 20:30h. Skeletal lengths of the head (skull + beak) and tarsus (notch of the intertarsal joint to the tarsometatarsal bone were measured daily with a digital caliper to the nearest 0.10 mm [48]. A digital caliper was also used to measure wing chord length of birds (bend of the wing to the tip of the longest primary feather) post-mortem.
Nestlings were forced-fed a moistened protein-enriched diet, as described in Lepczyk et al. [47] through blunt end syringes fifteen times a day. The mass per feeding was determined by previous knowledge determining the age-dependent energy needs of house sparrows [49,50] and all nestlings were force-fed the age-specific meal size regardless of treatment.

Experimental design
House sparrow nestlings were randomly assigned to one of two treatment groups (BCRV or Control), and nestlings from the same brood were randomly assigned different treatments groups. At 7 days of age, nestlings were inoculated subcutaneously on the breast with 75 μl containing 3 x 10 3 plaque forming units (PFU) of BCRV (lineage A, isolate H255A), or a buffered saline solution (Control) [n = 12 nestlings / treatment group]. The course of the infection was monitored for 4 days post inoculation (DPI), with four nestlings from each treatment euthanized at 2 DPI, 3 DPI, and 4 DPI. Nestlings were not kept past 4 DPI because BCRVinfected birds were unable to remain upright or swallow by 4 DPI and were euthanized for humane reasons no later than 4 DPI.

Sample collection and processing
Blood. Blood samples were collected daily from each nestling 0-4 DPI via jugular or brachial venipuncture. Blood samples were placed in serum separator tubes containing complete Dulbecco's Modified Eagle Media (DMEM), containing 10% fetal bovine serum, penicillin (100 U/ml), streptomycin (100μg/ml), and amphotericin B (0.25 μg/ml). Tubes were placed on wet ice, centrifuged, and then stored at -80˚C.
Oral swabs. The inner beak, oral cavity, and throat were swabbed with sterile, Dacron swabs daily post inoculation. The swab was placed into a tube containing 1 ml of complete DMEM media, placed on wet ice, and later transferred to storage at -80˚C.
Tissues. Four nestlings from each treatment group were euthanized by CO 2 overdose followed by cervical dislocation on 2 DPI, 3 DPI, and 4 DPI. Necropsies were performed, the gut was removed from each nestling, flushed with saline, measured to the nearest 0.1 centimeter, and blotted dry before being weighed. Subsamples of the skin, skeletal muscle, lung, heart, liver, small intestine, pancreas, spleen, bursa, kidney, cerebrum, and cerebellum were immediately frozen in liquid nitrogen from each nestling for future virological analysis. Subsamples of all tissues were also collected for pathology. Remaining intestinal tissue was flushed with cold PBS (pH 7.4), cut into proximal, medial, and distal sections, and then blotted dry before recording the mass and length of each section. Intestinal sections, as well as pancreas samples, were then stored at -80˚C until further analysis for digestive enzyme activity. Remaining muscle and liver samples were stored at -80˚C until further analysis for tissue maturation.

Plaque assay for analysis of viral loads
Levels of cytopathic BCRV were assessed in blood, swab, and tissue samples using a Vero plaque assay, according to [51]. Briefly, Vero cells in complete DMEM media were plated onto each well of a six-well plate and incubated for 3-4 days at 37˚C and 5% CO 2 . Diluted samples were added to each well and incubated for 45 min. After incubation, 3 mL of an agar overlay was added for plaque visualization. Plates were incubated at 37˚C and 5% CO 2 and checked for cytopathic effect at 72 h and 96 h. The number of plaques observed was recorded and virus concentrations were reported as PFU/ ml (blood, oral swabs) or PFU/ mg (tissues).

Pathology
Tissues were placed in 10% buffered formalin for two weeks, then they were trimmed, embedded in paraffin, sectioned at 5 μm, and stained with hematoxylin and eosin. Slides were then examined and blindly scored for histopathology at the USGS National Wildlife Health Center (Madison, WI).

Tissue maturation
Liver and muscle (flight + leg) samples were further analyzed for tissue composition. Wet masses of the liver and muscle samples were recorded (±0.10 mg) and dried to constant mass at 65˚C. Dry liver and muscle masses were recorded, water mass (g wet tissue-g dry tissue) was calculated, and dried liver and muscle samples were individually wrapped in pre-dried filter paper for lipid extraction in diethyl ether in a modified Soxhlet side-arm extractor. Lean dry mass and tissue mass extracted (g dry tissue-g lean dry tissue), as well as indices of muscle water content (g water/g lean dry tissue) and liver lipid content (g tissue extracted/g lean dry tissue) were calculated.

Digestive efficiency and excreta lipid content
Excreta was collected from each nestling starting 24 hours after arrival to the Animal Care Facility to allow for elimination of previously consumed food from the animals' digestive systems. Excreta was collected at hourly feedings directly from the bird or removed from its nest container. Excreta was then dried for 48 to 96 hours at 50˚C, weighed, then stored at -20˚C until further analysis. Dry excreta samples were used in digestive efficiency calculations and used to calculate excreta lipid content.
Digestive efficiency was measured according to [52]. Briefly, subsamples of food were dried for 24 hrs at 60˚C, and the daily mass intake was determined as wet mass ingested x percentage dry mass. Because endogenous wastes mix with undigested material in the cloaca, the assimilable mass coefficient (AMC; a measure of assimilation efficiency) is an underestimate of the true assimilable mass coefficient [53]. Therefore, we express digestive efficiency as an apparent AMC, which is calculated as (Qi −Qe)/Qi, where Qi = the rate of food intake (g of daily mass (DM)/d); and Qe = the rate of excreta production (g of DM/d; [54]).
For lipid content analysis, lipids were extracted from the dry excreta samples using diethyl ether in a modified Soxhlet side-arm extractor. Sample packets were dried again for 24 hours and then weighed to determine the percent lipid extracted.

Digestive enzymes
Intestinal brush border enzyme activity was assessed in whole tissue homogenates of the proximal, medial, and distal intestinal sections. Maltase and aminopeptidase-N were measured because they index the majority of brush border carbohydrate and peptide hydrolysis, respectively. Prior to analysis, samples were thawed and homogenized with cold 300 mM mannitol in 1 mM HEPES-KOH buffer (pH 7.5). Maltase activities were measured according to Dahlqvist [55] as modified by Martınez del Rio [56]. Briefly, gut homogenates were incubated with 56 mM maltose in 0.1 M maleate and NaOH buffer, pH 6.5, at 40˚C for 20 min. Next, stopdevelop reagent (glucose assay kit; Sigma-Aldrich, St. Louis, MO) was added to each tube, vortexed, and incubated at 40˚C for 30 min. Lastly, 12 N H 2 SO 4 was added to each tube, and the absorbance was read at 540 nm. Activity was normalized by intestinal wet mass.
Aminopeptidase-N activity was measured according to [50], with some modifications. Briefly, diluted gut homogenate was added to assay mix (2.0 mM L-alanine p-nitroanilide in 1 part 0.2 M NaH 2 PO 4 -Na 2 HPO 4 , pH 7.0, and 1 part deionized H 2 O) previously heated to 40˚C. The reaction solution was incubated for 25 min at 40˚C, ice cold 2 N acetic acid was added, and absorbance was measured at 384 nm. Apparent Michaelis constant (Km) and optimal pH for intestinal aminopeptidase-N activity were 1.1 ± 0.2mM (mean ± SE, n = 6) and 7.5, respectively. Activity was normalized per gram of intestinal wet mass.
To quantify pancreatic lipase activity, Quantichrom Lipase Assay Kit (BioAssay Systems, Hayward, CA) was used according to manufacturer's recommendations and the absorbance was read at 412 nm using a microplate reader.
To quantify pancreatic amylase activity, aliquots of pancreas homogenate (diluted in 20 mM of sodium phosphate buffer with 6.7 mM NaCl pH 6.9) were added to each tube. Next, 1% potato starch was added and incubated at 40˚C for 3 minutes. The reaction was stopped upon the addition of amylase color reagent to each tube. The tubes were placed in boiling water for 10 minutes and then cooled on ice for 3 minutes. Next, distilled water was added to the samples and the samples were read at 540 nm using a microplate reader.
To quantify pancreatic trypsin and chymotrypsin activity, pancreas samples were homogenized in 0.5 M Tris/HCl (pH 8.2) with 0.2 M CaCl 2 . Enterokinase (0.3%) was added to each sample and incubated for 1 hour at room temperature. Next, 1 mM BAPNA (N-alpha-benzoyl-dl-arginine-p-nitroanilide, pH 8.2) or GAPNA (N-glutaryl-l-phenylalanine-p-nitroanilide, pH 7.6) was added as the substrate for trypsin and chymotrypsin, respectively, and incubated for 10 minutes at 40˚C. Next, 30% acetic acid was added to each tube for termination of the reaction and read at 410 nm using a microplate reader.

Statistical analyses
JMP Pro 12 was used for all statistical analyses. A comparison of 2 DPI viral loads across tissues was performed using a linear mixed effect model with bird as a random effect. Comparisons of 3 DPI and 4 DPI viral loads across all tissues were not run due to low sample-sizes and non-normality of the data. Viral load in the cerebrum and lungs were analyzed for differences over time from 2 DPI to 4 DPI using one-way ANOVA. Analyses of viral load over time in the remaining tissues (heart, kidney, liver, muscle, skin, small intestine) were not run due to low sample sizes and non-normality of the data. Viral load data were log-transformed for analyses. Measures of body mass, head-beak length, tarsus length, AMC, and excreta lipid content from live control and BCRV-inoculated birds were compared by two-sample t-test at each day post inoculation (0 DPI, 1 DPI, 2 DPI, and 3 DPI). Data from 4 DPI are presented in figures but were not compared statistically due to low 4 DPI cohort sample size (n = 4 per group). Post-mortem measures of liver lipid content, muscle water content, wing chord, and digestive enzymes (pancreatic and intestinal) were compared by two-way ANOVA with infection treatment and DPI as factors. There were no significant interactions for any measure; interactions were dropped from the models before testing for fixed effects. In all tests, the significance level was set at α = 0.05.

Viral detection
Viremia was quantified using a Vero plaque forming assay (N = 12/treatment). No viremia was detected in the blood in any group at 0 DPI. BCRV was detected in the BCRV-infected group on 1 and 2 DPI, with peak viremia levels occurring at 1 DPI in BCRV-infected birds (1.7 x 10 4 ± 4.4 x 10 4 PFU/ml blood), and declining to 5.8 x 10 3 ± 2.8 x 10 3 PFU/ml blood by 2 DPI (data not shown). No viremia was detected in the blood on 3 or 4 DPI. Virus was detected in oral swabs of one bird on 1 DPI (1 PFU/ml swab fluid) and one bird on 4 DPI (2 PFU/ml swab fluid, data not shown).
The viral load of 8 different tissues (cerebrum, heart, kidney, liver, lung, small intestine, skin, and skeletal muscle) was determined post mortem for all birds 2-4 DPI (Table 1). BCRV was detected in all tissue types analyzed at 2 DPI and viral loads differed among tissues (F 7,21 = 61.28, p<0.0001). Viral load in the cerebrum was significantly higher than all other tissues at 2 DPI and load in the lungs was significantly higher than all tissues except the cerebrum (Tukey's p<0.001 for all significant pairwise comparisons). Viral load decreased from 2 DPI to 4 DPI in the cerebral tissues (F 2,9 = 5.01, p = 0.035, Tukey p = 0.049 comparing 2 DPI and 4 DPI) and the lung tissues (F 2,9 = 5.35, p = 0.030, Tukey p = 0.024 comparing 2 DPI and 4 DPI).

Pathology
Histopathologic abnormalities in BCRV infected birds were confined to the brain and meninges. All treatment birds developed mild to moderate, multifocal lymphoplamacytic and heterophilic encephalitis, as well as mild lymphoplasmacytic meningitis. These findings were most marked in the cerebrum and optic lobe, with the cerebellum and brainstem less affected. Encephalitis was characterized by multifocal lymphoplasmacytic cuffing (1-5 cell layers), perivascular edema, infiltration of the neuropil by heterophils, lymphocytes, macrophages, gliosis, and multiple necrotic neurons (Fig 1).
The most striking change in the cerebellum was edema of the Purkinje cell layer with occasional necrotic Purkinje cells. Gliosis and rarely, lymphoplasmacytic cuffing were present in the molecular layer of the cerebellum. No significant abnormalities were seen in the kidneys, heart, skin, liver, skeletal muscle, spleen, bursa of Fabricius, intestine, or ventriculus in control or BCRV-inoculated nestlings. No abnormalities were present in the brain or meninges of control nestlings.

Growth and maturation
Body mass and skeletal lengths were measured on live birds following inoculation. There were no significant differences in body mass between control and treatment groups at any day post inoculation (Fig 2A; 0DPI t 20 = 1.09, p = 0.289; 1DPI t 20 = 1.47, p = 0.157; 2DPI t 20 = 0.67, p = 0.508; 3DPI t 13 = 0.69, p = 0.501), likely due to the forced feeding protocol that was maintained throughout the experiment. However, BCRV-inoculated nestlings did show decreased skeletal development after inoculation, despite uniform feed consumption rates compared to controls. Head-beak length of BCRV-inoculated nestlings was significantly smaller than noninfected controls at 2 DPI (t 20 = 2.27, p = 0.034) and 3DPI (t 13 = 2.55, p = 0.024) (Fig 2B), and Effects of an alphavirus infection on avian physiology tarsus length was significantly smaller than non-infected controls at 3 DPI (t 13 = 2.92, p = 0.012) (Fig 2C).
Measures were taken from cohorts of birds post-mortem at 2, 3, and 4 DPI (n = 4 per group) to examine the impacts of infection on tissue maturation and feather growth. There were no significant interactions between DPI and treatment group for any measure. Liver lipid index was significantly lower in BCRV-infected birds compared with controls (Treatment F = 5.71, p = 0.027; DPI F = 0.35, p = 0.710) (Fig 3A). Muscle water index, a measure of functional immaturity of the tissue, did not significantly differ between BCRV-infected and control individuals, but significantly decreased with DPI as expected given the increasing age of the birds (Treatment F = 1.85, p = 0.189; DPI F = 4.45, p = 0.025) (Fig 3B). Wing chord (length of the wing feathers) significantly increased with DPI (DPI F = 31.41, p<0.001), and was significantly shorter in BCRV-infected birds compared with controls (Treatment F = 11.54, p = 0.003) (Fig 3C).

Digestive function
Excreta were collected daily to measure digestive function over the course of infection. While there were no significant differences in apparent AMC in the early days post-inoculation (0DPI and 1DPI: t 22 <0.93, p>0.364), BCRV-infected nestlings had significantly lower apparent AMC when compared to controls at 2 DPI (t 14 = 2.26, p = 0.041) (Fig 4A). Additionally, while there were no significant differences in excreta lipid content in the early days post-inoculation (0DPI and 1DPI: t 22 <2.00, p>0.058), BCRV-infected nestlings excreted significantly more lipid at 2 DPI when compared to controls (t 14 = 3.87, p = 0.002) (Fig 4B). Excreta and AMC measures were not compared at 3 DPI due to low sample size (n = 4 per group).
Assimilation organs were harvested from cohorts of birds post-mortem at 2, 3, and 4 DPI to examine the impact of BCRV infection of digestive enzyme activity. There were no significant interactions between DPI and treatment group for any measure. Amylase activity per gram of pancreas tissue was significantly lower in BCRV-inoculated birds compared with controls (Treatment F = 7.51, p = 0.013; DPI F = 0.11, p = 0.901) (Fig 5A), yet there were no significant differences in pancreatic lipase or trypsin activities (Treatment F<2.93, p>0.103; DPI F<1.77, p>0.197) (Fig 5B and 5C). Maltase activity per gram of intestinal tissue was significantly lower in BCRV-inoculated birds compared with controls (Treatment F = 9.08, p = 0.007; DPI F = 0.10, p = 0.387) (Fig 6A), yet there were no significant differences in aminopeptidase-N activity (Treatment F = 2.71, p = 0.116; DPI F = 1.01, p = 0.382) (Fig 6B).

Discussion
Alphaviruses such as Eastern and Western Equine Encephalitis virus, Chikungunya virus, and Sindbis virus are known to cause neuroinvasive disease in humans [57], especially in children and the elderly [28,58]. The majority of studies performed on age-dependent alphavirus susceptibility have been experimental alphavirus infections of mice or clinical reports of alphavirus infections in humans [31,32]. We determined the functional impact of an alphavirus infection on growth, maturation, and digestive function in nestling birds. To our knowledge, this is the first study to demonstrate the physiological impact of a viral infection on nestling altricial birds.

Effects of an alphavirus infection on avian physiology
Encephalitis is common in neuroinvasive alphavirus disease, but the mechanism of neuroinvasion has not been well studied in any animal model. Chikungunya virus may directly infect endothelial cells of cerebral blood vessels and gain access to the CNS through blood Effects of an alphavirus infection on avian physiology brain barrier disruption, whereas Sindbis virus appears to infect peripheral neurons and gain access to the CNS through axonal transport [57]. However, how these infection pathways change with age and allow for higher rates of neuroinvasion in the young and old is unknown. Additionally, although birds are hosts for at least seven alphaviruses, the pathogenesis of avian alphavirus infections is not well studied [59]. Here we report neuroinvasive disease in nestling house sparrows experimentally infected with BCRV. Encephalitis and high viral load were found in the cerebra of all infected nestlings. No apparent pathology was found outside the central nervous system in the organism, although significant impairment of growth, maturation, and digestive function was observed.
The nestling period of birds is an energetically expensive time during which rapid anatomical growth and maturation occurs. Given that infectious diseases can be costly to an organism in terms of pathological damage, decreased appetite and impaired growth, the nestling period of a bird's life is likely to be especially susceptible to the impacts of infectious diseases. BCRVinfected birds showed no significant difference in body mass compared with control birds due to a strict meal size matching between treatment groups and force-feeding of infected birds when they were demonstrating morbidity. Despite similar whole body wet masses between treatment groups, significant reductions in head-beak length and tarsus length were observed by 3 days post-inoculation in infected nestlings. Additionally, BCRV infection led to significantly reduced wing chord length in nestlings.
Our findings of reduced tarsus, head-beak, and wing lengths during infection are particularly striking given that numerous studies have shown prioritization of skeletal growth and feather growth relative to mass gain in nestlings faced with acute or chronic food restriction [48,60,61,62,63,64]. It has been posited that maintenance of skeletal growth occurs proximally due to strict endocrine control of the skeletal growth program, and that ultimately larger structural size improves competitive abilities and fledging success in altricial birds. Flight ability has also been shown to be prioritized during developmental stress [65,66,67]. Wing length is particularly important for fledging and foraging success and predator avoidance post-fledging [63,68,69]. Allocation of resources to wing growth can mitigate the negative effects of a small body size on fledging time [44]. In our study BCRV-infection clearly altered a typically well-preserved and adaptive developmental program of skeletal and wing growth in nestlings and this altered growth would likely have negative ecological consequences for any nestlings surviving infection in the wild. Indeed, field studies of natural BCRV infections in house sparrow nestlings have reported the detrimental impacts of BCRV on survival and fledgling success [17,70,39]. Specifically, house sparrow nestlings found infected with BCRV at a young age (less than 12 days of age) often exhibit higher viremia, higher mortality, and reduced fledging success compared to those nestlings infected at older ages [71]. The immune system of house sparrows develops during the nestling and post-fledging periods [37,38]. We hypothesize that younger nestlings are more susceptible to alphavirus neuroinvasion than older nestlings and that this neurological impairment results in impaired growth and physiological functions, further contributing to the poor survival of the infected nestlings.
Nestling development is closely linked to digestive function, and although the timecourse for development of the digestive system and body size and composition in altricial nestlings has been documented under normal conditions and in conditions of altered food availability and quality [47,48], no studies exist which have examined the development of nestling birds during microparasite infection, or how digestive function is affected during infection. Digestive function declined in our infected birds within two days of infection. Specifically, despite having the same food intake as controls, BCRV-infected nestlings had decreased digestive efficiency (decreased AMC). This decrease in digestive efficiency could result in a reduction of assimilable resources available for skeletal and wing growth. Decreased AMC may partially be due to reduced expression of pancreatic amylase and intestinal maltase enzymes, thereby preventing carbohydrate digestion, absorption, and use in growth processes. Additionally, an increased excretion of ingested lipid and reduced liver lipid content were observed in infected birds. When birds have excess resources, they are stored as fat rather than allocated to growth of excessively large organs [43]; excessive excretion of lipid and reduced enzymatic function indicates that infected birds did not have extra nutritional resources to store. Reduced nutritional resources in infected birds may prevent birds from mounting sufficient immune responses or from repairing damaged tissue that results from viral invasion or inflammation.

Conclusions
Our work demonstrates that arbovirus infections during a critical period of growth and development in young nestlings impairs digestive function and skeletal and feather growth. This impairment likely contributes to the poor fledging success recorded for BCRV-infected house sparrow nestlings in the wild and exemplifies the complex tradeoffs that can occur between immune system function and other physiological processes during periods of critical energetic needs in an organism's life. Future work in this area should focus on elucidating the mechanism through which neuroinvasive alphavirus disease impairs growth and development in young animals.