Diagnostic accuracy of antigen-based immunochromatographic rapid diagnostic tests for the detection of Salmonella in blood culture broth

Background In low resource settings, Salmonella serovars frequently cause bloodstream infections. This study investigated the diagnostic performance of immunochromatographic rapid diagnostic tests (RDTs), which detect Salmonella antigens, when applied to stored grown blood culture broth. Material/Methods The SD Bioline One Step Salmonella Typhi Ag Rapid Detection Kit (Standard Diagnostics, Republic of Korea), marketed for the detection of Salmonella enterica serovar Typhi (Salmonella Typhi) in stool and the Salmonella Ag Rapid Test (Creative Diagnostics, USA), marketed for the detection of all Salmonella serotypes in stool, were selected for evaluation based on a pre-test evaluation of six RDT products. The limits of detection (LOD) for culture suspensions were established and the selected RDT products were assessed on 19 freshly grown spiked blood culture broth samples and 413 stored clinical blood culture broth samples, collected in Cambodia and the Democratic Republic of the Congo. Results The LOD of both products was established as 107−108 CFU/ml. When applied to clinical blood culture broth samples, the diagnostic sensitivity and specificity of the SD Bioline RDT were respectively 100% and 79.7% for the detection of Salmonella Typhi; 94.4% (65/69) of false-positive results were caused by Salmonella Enteritidis. When considering the combined detection of Salmonella Typhi and Enteritidis (both group D Salmonella), sensitivity and specificity were 97.9% and 98.5% respectively. For Creative Diagnostics, diagnostic sensitivity was 78.3% and specificity 91.0% for all Salmonella serotypes combined; 88.3% (53/60) of false negative results were caused by Salmonella Paratyphi A. Conclusions When applied to grown blood culture broths, the SD Bioline RDT had a good sensitivity and specificity for the detection of Salmonella Typhi and Salmonella Enteritidis. The Creative Diagnostics product had a moderate sensitivity and acceptable specificity for the detection of all Salmonella serovars combined and needs further optimization. A RDT that reliably detects Salmonella Paratyphi A is needed.


Introduction
In low and middle income countries, invasive Salmonella infections continue to pose an important public health problem.Enteric fever, i.e. a bloodstream infection with Salmonella enterica serovar Typhi (Salmonella Typhi) or serovar Paratyphi A, B and C (Salmonella Paratyphi), has an estimated incidence of 27 million cases and causes 190,200 (uncertainty range 23,800-359,100) deaths per year [1,2].Non-typhoidal Salmonella serovars, in particular Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) and Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) cause another 3.4 million cases of bloodstream infections and an estimated 681,316 (uncertainty range 415,164-1,301,520) deaths annually, mainly in sub-Saharan Africa [3].
Symptoms associated with these invasive infections are non-specific and laboratory diagnosis remains challenging.Blood culture is often used as the reference method but it shows poor sensitivity (40-80%) and is technically and financially demanding [4,5].In addition, the time to diagnosis is 2-3 days.
Other diagnostic tests like the serological Widal test or PCR-based tests suffer from low sensitivity and delayed positivity or are of little use in resource constrained settings [6].Furthermore, incorrect use and interpretation of serological tests frequently leads to overdiagnosis and overtreatment [7].
A rapid diagnostic test that is affordable and reliable is thus urgently needed and recently developed rapid diagnostics tests (RDTs) based on Salmonella antigen detection seem promising.These RDTs are currently only marketed for application on stool, serum, plasma and/or whole blood and not for application on grown blood culture broth.Castonguay-Vanier et al. successfully applied one of them to blood culture broth speeding up the time to diagnosis of Salmonella Typhi infections with one to two days [8].However, they only included samples from a single center in Laos and did not assess the detection of other frequent Salmonella serovars or possible cross-reactivity with Gram-positive organisms.
The present study aims to evaluate the performance of antigen-based immunochromatographic rapid diagnostic tests for the detection of the four most common Salmonella serovars when applied to blood culture broth from patients from Cambodia and the Democratic Republic of Congo (DRC).

Study design
The study assessed six RDT products in three steps: (i) a proof-of-concept evaluation with determination of limit of detection (LOD); (ii) evaluation on spiked blood culture broth samples and (iii) evaluation on stored grown blood culture broth samples obtained during routine patient care in Cambodia and the DRC.The reference method was standard microbiological work-up of blood cultures; serological testing was not performed at both study sites.Reporting of the methods and results was done according to the STARD guidelines for diagnostic studies [9].

Rapid diagnostic test products
All six RDT products assessed, their targets and abbreviations as used throughout the text are listed in Table 1.Except for the SMI Paratyphi A RDT and the SD Bioline RDT, all products are commercially available.The products are not officially marketed for application on broth.The present study is a validation study to confirm that these products can be reliably used on grown blood culture broth and can therefore speed up the time to diagnosis of invasive Salmonella infections; it does not concern a prospective clinical validation of these products.
Included were five cassettes and one dipstick format (the SMI Typhi RDT).Per product at least two lot numbers were included.All RDT kits had been stored in compliance with the temperature stability mentioned on the box.Results were read at the minimum and maximum reading time of the reading interval described in the Instructions For Use (IFU).A test result was considered invalid when the control line was not visible.Test lines intensity was scored as 'faint' (hardly visible) and 'weak', 'medium' or 'strong' if intensity was weaker, equal or stronger compared to the control line.

I Proof-of-concept evaluation with determination of limit of detection
Salmonella Typhi 00032304 (strain confirmed at the Belgian Reference Laboratory for Salmonella, Scientific Institute of Health (WIV-ISP), Etterbeek, Belgium), Salmonella Paratyphi A ATCC 9150, Salmonella Enteritidis ATCC 13076 and Salmonella Typhimurium ATCC 14028 were grown overnight.Two suspensions of pure culture with a concentration of 1.5 x 10 8 colony forming units/ml (CFU/ml) were made, one in sterile Phosphate Buffered Saline (PBS) and one in blood culture broth.Then, serial 10-fold dilutions where made to obtain a range of 1.5 x 10 8 CFU/ml to 1.5 x 10 3 CFU/ml.Blood culture broth was obtained from blood culture bottles (BacT/ALERT FA/FAN-bioMe ´rieux, Marcy L'Etoile, France) which had been inoculated with 10 ml fresh whole blood from a healthy person.The accuracy of the dilutions was verified by colony count.The volumes applied to the RDTs were in compliance with the IFU of each product.In case number of drops instead of exact volumes were mentioned, exact volumes were calculated by weighing the drops applied by the kit's transfer device.All samples were tested in duplicate.
As blood culture broth was not listed as a possible specimen for any of the RDT products tested, the sample preparation procedure was optimized.The following sample preparation steps were tested: (i) pre-incubation at 100˚C for 5 minutes (PBS); (ii) centrifugation for 1 minute at 500 x g (blood culture broth) [8]; and (iii) dilution in buffer included in the test kits (blood culture broth and PBS).

II Application on spiked blood culture samples
Two RDT products were selected for further testing: The SD Bioline RDT and the Creative Diagnostics RDT.A panel of reference and clinical strains was made including six Salmonella serovars and 13 other pathogens and contaminants commonly isolated from blood cultures or with possible cross-reactivity (Table 2).Freshly grown colonies were suspended in sterile saline at 0.5 McFarland (which equals 1,5 x 10 8 CFU/ml).The estimated bacterial concentration after serial dilutions were performed was 375 CFU/ml.Then, 500 µl of this bacterial suspension was used to spike blood culture bottles (BacT/ALERT FA/PF), to which defibrinated sheep blood (ref.8545104, International Medical Products, Marche-en-Famenne, Belgium) had been added.The blood culture bottles were incubated at 35˚C in a BacT/ALERT1 3D 60 machine   ).Tests were performed on the same day.Colony counts were performed to estimate the concentration of bacteria present in the grown blood culture broth.
The calculated concentrations varied between 10 7 −10 10 CFU/ml depending on the organism (S1 Table ).The manufacturers' instructions were modified in accordance to the optimizations tests performed: 1.5 ml of grown blood culture broth was centrifuged for 1 min.at 500 x g in a 1.5-ml tube.A calibrated micropipette was used to transfer the supernatant to the RDT products.For the SD Bioline RDT, 100µl of supernatant was transferred.For the Creative Diagnostics RDT, application of 100µl of supernatant was compared to the application of 50 µl of supernatant with addition of 4 drops of provided buffer.Results of the SD Bioline RDT were read after 10 and 20 minutes as the IFU reported a reading window of 10-20 minutes.The result of the Creative Diagnostics RDT were read at 10 minutes as mentioned in the IFU.

III Application on stored grown blood culture broth
A selection was made out of a collection of blood culture broth samples previously collected and stored at -80˚C at the Institut National de Recherche Biome ´dicale (INRB) in Kinshasa, DRC, and the Sihanouk Hospital Center of HOPE (SHCH) in Phnom Penh, Cambodia.Both institutes conduct microbiological surveillance in collaboration with the Institute of Tropical Medicine (ITM) in Antwerp, Belgium.At SHCH, blood (2 x 10 ml) was cultured in BacT/ALERT culture bottles (paired aerobic (FA) and anaerobic bottles (FAN), bioMe ´rieux, Marcy l'Etoile, France).Blood cultures were worked up as previously described [14].The majority of samples were tested on site, a selection of samples were tested at ITM, Antwerp, Belgium, after shipment on dry ice.In DRC, 1-4 mL of blood was sampled into pediatric blood culture bottles (BacT/ALERT PF) for children ( 14 years old), and 2 × 10 mL into aerobic adult blood culture bottles (BacT/ALERT FA).Blood cultures were processed as described elsewhere [15].All samples from DRC were shipped to Belgium on dry ice for testing.
The blood culture broth samples comprised three groups: (i) Salmonella positive samples; (ii) samples positive for competing organisms; and (iii) non-grown blood cultures.All tests were performed between August 2016 and January 2017.For this step, 1.5 ml of grown blood culture broth was centrifuged for 1 min.at 500 x g in a 1.5-ml tube and 100µl supernatant was applied to both RDT products.All results were read by two readers.One of the readers had access to the blood culture test results, but sample numbers were coded before testing.The second reader had no access to the blood culture results and the two readers were blinded to each other.In case of discordance, the final result was based on consensus.
In case of false positive or false negative RDT results, the blood culture broth was plated onto sheep blood agar and incubated overnight and next re-identified by standard biochemical methods.In case the re-identification was different from the identification recorded in the database, the sample was excluded from subsequent analysis.

Evaluation of instructions for use (IFU) and readability of test lines
The information mentioned in the IFU of the RDT products was assessed using a checklist that was based on a generic template for IFUs for malaria RDTs from a previous study [16].In addition, cassette design and the ease of reading test lines was assessed.

Sample size calculation
To achieve an estimated sensitivity of 95% with an accuracy of 0.05, 73 samples containing Salmonella spp.(target Creative Diagnostics RDT) and 73 samples containing Salmonella Typhi (target SD Bioline RDT) were needed.To achieve an estimated specificity of 90% with an accuracy of 0.05, 139 controls, meaning samples other than those positive for Salmonella spp.and/ or Salmonella Typhi were needed.The control group included samples with: (i) other Salmonella serotypes, (ii) isolates belonging to the most frequently isolated pathogens and contaminants at the respective study sites in Cambodia and the DRC (iii) isolates of species with potential cross-reactivity.

Statistical analysis
Sensitivity and specificity of the RDT products compared to the standard test (blood culture) were calculated with 95% confidence intervals (CI) using Stata 12 (Stata Corp., College Station, TX, USA).Inter-observer agreements for test line intensities and positive and negative test results were expressed by the percentage of overall agreement and by kappa values for each pair of readers.Reader one was the same lab technician for all samples, while the second reader was either a lab technician based in Cambodia ('reader 2') or in Belgium ('reader 3').

Ethical review
The microbiological surveillance study through which blood culture broth was sampled and stored was approved by the Institutional Review Board (IRB) of ITM, the Ethical Committee of Antwerp University, Belgium, the National Ethics Committee for Health Research (NECHR), Cambodia and the ethics committee of the 'E ´cole de Sante ´Publique' of the University of Kinshasa, DRC.In addition, specific approval was requested for the use of these stored blood culture samples for the evaluation of antigen-based Salmonella rapid diagnostic tests and which was granted by the IRB of ITM (ref.No consent was asked from patients as it concerned coded left-over samples collected through routine care.

I Proof-of-concept evaluation with determination of limit of detection
To determine the LOD for each product and its intended target (s), a Salmonella dilution series (range 1.5 x 10 8 CFU/ml to 1.5 x 10 3 CFU/ml) was applied to each RDT product.The results are shown in Table 3.In the IFU of three out of six RDT products, no information was mentioned about analytical sensitivity.For the SD Bioline RDT a LOD of 10 5 CFU/ml for Salmonella Typhi was mentioned and for Creative Diagnostics this was 10 7 CFU/ml for Salmonella Typhi and 10 4 CFU/ml for Salmonella Enteritidis and Salmonella Typhimurium.For the Dialab RDT an analytical sensitivity of 25 ng/ml LPS for Salmonella Typhi and Paratyphi A was mentioned without stating a conversion factor to CFU/ml.
The SD Bioline RDT (detection of Salmonella Typhi) performed best with a LOD for Salmonella Typhi of 10 7 −10 8 CFU/ml, no invalid test results and no false positive results, for both PBS and blood culture broth.The Creative Diagnostics RDT also performed satisfactory with an LOD of 10 7 CFU/ml for Salmonella Enteritidis and 10 8 CFU/ml for Salmonella Typhi, Salmonella Paratyphi A and Salmonella Typhimurium.The other tests performed unsatisfactory (no test line visible at 10 8 CFU/ml) and we therefore proceeded the analysis with the SD Bioline RDT and the Creative Diagnostics RDT.

II Application on spiked blood culture samples
Nineteen blood culture bottles were spiked with a panel of reference and clinical strains including six Salmonella serovars and 13 other pathogens (Table 2).All bacteria grew in the blood culture bottles within 24 hours.
For the SD Bioline RDT (targeting Salmonella Typhi) the blood culture broth samples containing Salmonella Typhi, Salmonella Enteritidis and Salmonella enterica serovar Jamaica (all serogroup D) tested positive.The remaining blood culture samples containing Salmonella serotypes other than serogroup D or competing organisms tested negative.There were no invalid test results.
The Creative Diagnostics RDT showed a visible test line for all Salmonella serovars except Salmonella Concord (serogroup C1) and Salmonella enterica subspecies diarizonae.Application of 100 µl of supernatant and no buffer (results shown in Table 2) resulted in two samples with a false positive reading, those containing Proteus spp.and Citrobacter freundii.One invalid result occurred (sample containing Klebsiella pneumoniae) due to poor background clearing.Addition of buffer resulted in better background clearance but also in more false-positive readings and was therefore discontinued.

III Application on stored grown blood culture broths
In total 413 clinical blood culture broth samples from 413 patients were thawed and subsequently applied to both RDT products (  patients with a Salmonella infection was 4 years (IQR 1-25 years) and 54.9% (152/277) were male; the median age of the patients without a Salmonella infection was 29 years (IQR 2-60 years) and 46.2% (61/132) were male.Thirteen (3.1%) samples were excluded from subsequent analysis as they gave false negative or false positive results with growth of a pathogen different from the one recorded in the database upon subculture of the thawed blood culture broth (S3 Table ).The 413 broth samples included for the final analysis comprised 240 samples from DRC and 173 from Cambodia; 277 samples had been tested positive with Salmonella; 123 had grown other competing bacteria and 13 were samples without growth (Table 4).
Invalid test results were observed only twice, both for Creative Diagnostics.These invalid results were due to very poor background clearance.One SD Bioline blister contained a discolored (green) desiccant (indicating humidity saturation) and was subsequently replaced by another cassette.
The degree of background clearance was more associated with the geographical origin of the samples than with the RDT products.Samples from Cambodia generally had a poorer background clearance compared to samples from DRC (Fig 1).We hypothesize that this might be caused by (inadvertent) repeat freeze-thawing caused by occasional power failure at the DRC site.Reading of results in case of poor background clearance was more difficult for the Creative Diagnostics RDT, probably due to the weaker intensity of control-and test lines.
Of note, occurrence of faint black test lines or combined red and black lines were occasionally observed (Fig 2).The IFU's of both products did not mention a possible presence of black colored test lines or combined black and red colored test lines.For the current evaluation, faint black test lines were reported as negative; black test lines that were scored as weak, medium or strong and test lines with a combined red and black color were reported as positive.Occurrence of black or combined red and black lines were mostly seen for samples from DRC and for samples containing Salmonella Typhimurium.
For the SD Bioline RDT, overall agreement and kappa values between pairs of observers were excellent for both positive and negative readings (! 97.9%, kappa values !0.95) and for line intensity readings (! 96.1%, kappa values !0.93) (S4 Table ).All but one discordance in line intensity occurred within one category of difference.For Creative Diagnostics, agreement was good for positive and negative readings (! 96.2%, kappa values !0.92) and moderate for line intensity readings (! 86.0%, kappa values !0.80) (S4 Table ).All but two discordances in line intensity occurred within one category of difference.

Evaluation of instructions for use (IFU) and readability of test lines
The information mentioned in the IFU of all 6 RDT products was assessed (S5 Table ); the most striking findings are mentioned here.All of the 6 RDT kits contained an IFU of which one had an additional job aid (SD Bioline).Version numbers and dates of issue were both missing in 3 out of 6 IFUs.Only one IFU clearly mentioned the target antigen (Dialab RDT).Except for the SD Bioline RDT kit, detailed information on the performance of the RDT products was lacking.Three IFUs did not provide any information on sensitivity or specificity; two IFUs gave vague indications of sensitivity and specificity such as 0 Sensitivity: ACCUCARE S. typhi-S.paratyphi assay was run using serum and stool samples versus culture positive samples and found to give positive results in all cases' (Labcare RDT).Some IFUs contained major errors, like the SMI Typhi RDT IFU which mentioned that it detected Salmonella Typhimurium instead of Salmonella Typhi.Of note, the Creative Diagnostics IFU mentioned a green colored test line while we observed a red colored test line for both the control and test line.
For reading of results, SD Bioline was preferred compared to Creative Diagnostics.Controland test lines had a stronger intensity, and test lines remained visible for more than 24 hours.For the Creative Diagnostics RDT, positive test lines were often faint to weak and the visibility of the lines was heavily influenced by variable background clearance.

Discussion
In this study, the performance of immunochromatographic rapid diagnostics tests for the detection of Salmonella antigens in blood culture broth was evaluated.
The SD Bioline RDT showed a sensitivity of 100% which is in line with the 96.7% previously observed for this product in Laos [8].The specificity of 80.2% was lower due to the inclusion of a high number of Salmonella Enteritidis positive samples.The positive results for samples containing serogroup D Salmonella adds evidence to the assumption of Castonguay et al., that this product probably detects the O9 antigen [8].The specificity of the SD Bioline RDT for the detection of group D Salmonella combined (98.9%) was much better compared to Salmonella Typhi alone.Two out of three false-positive results occurred with samples containing probable contaminants and it cannot be excluded that group D Salmonella might have been present in the blood at the time of sampling.
The LOD of the SD Bioline was determined to be 10 7 −10 8 CFU/ml for Salmonella Typhi which was lower than mentioned in the IFU (10 5 CFU/ml) but which is in line with the estimated minimal bacterial concentrations required for blood cultures to be flagged positive 10 7 −10 8 CFU/ml) [17].
The Creative Diagnostics RDT had not been evaluated previously.In the present study, a sensitivity and specificity of 78.3% and 91.0% were found.The highest proportion of false negative results were observed for samples containing Salmonella Paratyphi A. A possible explanation might be that the test detects the O12 antigen.This antigen, which can be subdivided into subtypes 12 1 , 12 2 , and 12 3 , is present in Salmonella Typhi, Typhimurium and Enteritidis and absent in Salmonella Choleraesuis and Salmonella Concord.Salmonella Paratyphi A carries only subtypes 12 1 and 12 3 [18].Tam et al. noted that the O12 antibody is less immuno-dominant than O9 (or O2) and that although most typhoid patients produce vast amounts of anti-O12 antibodies, many paratyphoid patients produce little or none at all [19].In addition, PCR experiments showed that the median number of copies of target DNA per ml of blood was 39 for Salmonella Paratyphi A compared to 60 for Salmonella Typhi which suggests a lower concentration in blood during infection [20,21].
The LOD was determined to be 10 8 CFU/ml for Salmonella Typhi, Paratyphi A and Typhimurium and 10 7 CFU/ml for Salmonella Enteritidis.Similar to the SD Bioline RDT, these LODs are lower compared to what is mentioned in the IFU for detection in stool.Of note, three Salmonella Typhi positive samples that gave no visible test line with the Creative Diagnostics RDT, did give a clearly visible test line with the SD Bioline which could indicate a slightly lower LOD of the Creative Diagnostics RDT in practice.The 12 false positive results were caused by samples confirmed to contain a range of clinically significant pathogens such as Escherichia coli and Klebsiella pneumoniae and is thus of concern.
The remaining four RDT products did not reliably detect Salmonella in the highest concentration applied (10 8 CFU/ml) in PBS and blood culture medium.This raises questions regarding the accuracy of these RDT products on blood samples (serum, plasma, whole blood), as the concentration of Salmonella Typhi and Salmonella Paratyphi A in blood during acute infection is estimated to be only 1 CFU/ml [5,22].In addition, the quality of information provided in the IFU's of these products was generally poor.
The present study has some limitations.First, clinical blood culture broth samples were tested retrospectively after storage times up to 7 years.Second, freezing and thawing of samples might have negatively influenced the test results due to deterioration of the samples.Last, it was not possible to reconfirm the identification of all blood culture broth samples that gave false results, either because bacteria could not be recovered from samples or because of assumed overgrowth by contaminants.
Apart from these limitations, this study also has several strengths.We assessed the use of Salmonella RDT products for the detection of several Salmonella serotypes, which is relevant since the epidemiology of invasive Salmonella infections is changing globally.In addition, the possible cross-reactivity of a range of competing pathogens was assessed.Last, samples from two geographically distinct settings, using different blood culture bottles, were used.Both settings are endemic for invasive Salmonella infections.
The use of an RDT on grown blood culture broth has the advantage of speeding up the diagnosis of invasive bloodstream infections, or even improving detections rates [8,23,24].However, in Asia the addition of the SD Bioline RDT to the routine work-up of blood culture samples would likely not be of major benefit.Despite its overall good performance, the product is unable to detect Salmonella Paratyphi A which in some Asian countries now causes more infections than Salmonella Typhi [25].The product would also not be suited for use in sub-Saharan Africa as it is not able to detect Salmonella Typhimurium which is commonly isolated from blood cultures in this setting [15].
The Creative Diagnostics RDT might be of use in sub-Saharan Africa as it was able to detect nearly all samples positive with Salmonella Typhi, Enteritidis and Typhimurium, the serotypes that cause nearly all Salmonella bloodstream infections in this setting [15].The bad performance with Salmonella Paratyphi A and Salmonella Choleraesuis positive samples limits its use in Asia.In addition, the operational characteristics of Creative Diagnostics RDT (line intensities, line stability) need to be improved.
In general, the need for grown blood culture broth requires the presence of an adequately equipped laboratory.Although the time to diagnosis can be shortened with 1-2 days future studies should evaluate if point of care testing is possible.Of concern is the fact that a percentage of negative samples gave black test lines, which might be attributed to the presence of charcoal in the culture media of the blood culture bottles used in this study.Further validation with other types of blood culture bottles is thus needed.Although easily distinguishable from true positive test lines by trained readers, misinterpretation in the field is likely.
The present study shows that new RDT products for the detection of invasive Salmonella infections need to be developed, in particular for the reliable detection of Salmonella Paratyphi A. Operational characteristics, instructions for use and specificity of existing products should be further optimized.

Conclusion
The SD Bioline RDT had an excellent sensitivity and specificity for the detection of Salmonella Typhi and Salmonella Enteritidis in grown blood culture broth.The Creative Diagnostics RDT had a moderate sensitivity for the detection of Salmonella spp.and an acceptable specificity; it particularly failed to detect Salmonella Paratyphi A.

Fig 1 .
Fig 1. Variable background clearance.Both tests shown (Standard Diagnostics Bioline One Step Salmonella Typhi Ag Rapid Detection Kit) with the presence of a control line and absence of a test line, show a negative test result.Background clearance is scored poor for the test above (more reddish) and good for the test below.https://doi.org/10.1371/journal.pone.0194024.g001

Fig 2 .
Fig 2. Presence of black and red colored test lines.Samples applied to both tests (Salmonella Ag Rapid Test, Creative diagnostics) contained Salmonella Enteritidis and both were scored as a positive test result.The test above shows a red colored control line and a black colored test line.The picture below shows a red colored control and test line.https://doi.org/10.1371/journal.pone.0194024.g002

Table 1 . Characteristics of the Salmonella antigen-based immunochromatographic rapid diagnostic tests evaluated. Abbreviation used in main text Product name Manufacturer Specimen No. of bands Antigens targeted according to Instructions For Use (IFU)
[8]lthough the IFU states that the target of the test is 'monoclonal Salmonella Typhi antigen', the results as published by Castonguay et al.[8], suggest that the test targets the O9 antigen.This antigen can be found in all Salmonella serovars of serogroup D. a

Table 3 . Determination of limit of detection and corresponding test line intensity for each rapid diagnostic test. LOD for Salmonella Typhi (CFU/ml) LOD for Salmonella Paratyphi A (CFU/ml) LOD for Salmonella Typhimurium (CFU/ml) LOD for Salmonella Enteritidis (CFU/ml)
8= No test line visible at the highest concentration tested (10 8 CFU/ml) Ã = Faint test lines were visible at random concentrations, but at the highest concentration (10 8 CFU/ml) no test line was visible / = In case of different results between parallel testing both results are mentioned with a "/" in between CFU = Colony Forming Units; F = faint; LOD = Limit Of Detection; M = medium; PBS = Phosphate Buffered Saline; S = strong; W = weak https://doi.org/10.1371/journal.pone.0194024.t003