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Douglas Carvalho Caixeta, Renata Roland Teixeira, Leonardo Gomes Peixoto, Helen Lara Machado, Nathalia Belele Baptista, Adriele Vieira de Souza, Danielle Diniz Vilela, Celso Rodrigues Franci, Foued Salmen Espindola
Adaptogenic potential of royal jelly in liver of rats exposed to chronic stress
www.plosone.org
www.plosone.org
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229.2094 94.9039 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(emission\).)Tj
4.6771 0 Td
(A)Tj
0.9071 0 Td
(MDA)Tj
2.5285 0 Td
(standard)Tj
3.7247 0 Td
(curve)Tj
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(allowed)Tj
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(the)Tj
1.4683 0 Td
(quantification)Tj
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(the)Tj
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(compound)Tj
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(linear)Tj
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(regression)Tj
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([)Tj
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(25)Tj
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(].)Tj
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(TBARS)Tj
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(levels)Tj
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(calculated)Tj
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(of)Tj
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(antioxidant)Tj
5.0626 0 Td
(capacity)Tj
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(\(FRAP\).)Tj
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(reduce)Tj
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ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 342.3118 672.8314 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
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(+3)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 352.4031 668.4094 cm
BT
/F7 1 Tf
1 TL
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(to)Tj
1.0261 0 Td
(Fe)Tj
ET
Q
q
1 j
1 J
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BT
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1 TL
-0.0053 Tc
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Q
q
1 j
1 J
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BT
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(TPTZ)Tj
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(\(2,4,6-Tris)Tj
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(\(2-pyridyl\)-s-triazine\))Tj
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BT
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1 TL
-0.0053 Tc
0 0 Td
(+2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 462.8409 655.4267 cm
BT
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1 TL
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(-TPTZ)Tj
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(complex)Tj
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([)Tj
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(25)Tj
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(dismutase)Tj
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(\(SOD\))Tj
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(activity.)Tj
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(the)Tj
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(inhibition)Tj
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(capacity)Tj
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(pyrogallol.)Tj
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(SOD)Tj
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(activity)Tj
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(SOD)Tj
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(standard.)Tj
3.9457 0 Td
(A)Tj
0.907 0 Td
(50%)Tj
1.9276 0 Td
(inhibition)Tj
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(of)Tj
-35.4781 -1.2983 Td
(autoxidation)Tj
5.3007 0 Td
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(pyrogallol)Tj
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(defined)Tj
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(one)Tj
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(SOD)Tj
2.177 0 Td
(unit)Tj
1.8652 0 Td
([)Tj
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(25)Tj
0 g
(].)Tj
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-21.0159 -1.2982 Td
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3.8097 0 Td
(glutathione)Tj
5.0287 0 Td
(\(GSH\).)Tj
/F7 1 Tf
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1.7802 0 Td
(protein)Tj
3.1521 0 Td
(content)Tj
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(the)Tj
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(samples)Tj
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(precipitated)Tj
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(metaphosphoric)Tj
6.7237 0 Td
(acid)Tj
1.8709 0 Td
(\(MPA\))Tj
3.0387 0 Td
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(the)Tj
1.4684 0 Td
(ratio)Tj
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(of)Tj
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(1:1)Tj
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(\(homogenate/MPA\).)Tj
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(cen-)Tj
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(7000xg)Tj
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(10)Tj
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(minutes.)Tj
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(collected)Tj
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(\(100)Tj
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(8.0\),)Tj
1.9616 0 Td
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(EDTA)Tj
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(\(5mM\))Tj
3.0898 0 Td
(and)Tj
1.729 0 Td
(ortho-phthaldialdehyde)Tj
9.6944 0 Td
(\(1)Tj
-36.4305 -1.3039 Td
(mg/mL)Tj
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(in)Tj
1.0374 0 Td
(methanol\).)Tj
4.5865 0 Td
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1.7858 0 Td
(mixture)Tj
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(incubated)Tj
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(the)Tj
1.474 0 Td
(dark)Tj
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(room)Tj
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(15)Tj
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(350)Tj
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(\(excitation\))Tj
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(\(emission\).)Tj
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(mM\))Tj
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(linear)Tj
2.5114 0 Td
(regression)Tj
4.32 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
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-23.0455 -1.2983 Td
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(peroxidase)Tj
4.7169 0 Td
(\(GPx\))Tj
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(activity.)Tj
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1.338 0 Td
(measure)Tj
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(the)Tj
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(glutathione)Tj
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(peroxidase)Tj
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(activity,)Tj
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(buffer)Tj
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(\(100)Tj
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(mM)Tj
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(1)Tj
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(EDTA,)Tj
3.0444 0 Td
(pH7.7\),)Tj
3.2485 0 Td
(sodium)Tj
3.2258 0 Td
(azide)Tj
2.2904 0 Td
(\(40)Tj
1.508 0 Td
(mM\),)Tj
2.5001 0 Td
(GSH)Tj
2.177 0 Td
(\(diluted)Tj
3.3789 0 Td
(in)Tj
1.0375 0 Td
(5%)Tj
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(metaphosphoric)Tj
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(acid\),)Tj
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(GR)Tj
-34.9565 -1.2982 Td
(\(diluted)Tj
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(in)Tj
1.0318 0 Td
(GPx)Tj
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(buffer\),)Tj
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(NADPH)Tj
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(\(diluted)Tj
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(with)Tj
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(sodium)Tj
3.2315 0 Td
(bicarbonate)Tj
4.9435 0 Td
(5%\))Tj
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(and)Tj
1.7292 0 Td
(tert-butyl)Tj
3.9968 0 Td
(\(0.5)Tj
-34.3329 -1.304 Td
(mM\).)Tj
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(The)Tj
1.7858 0 Td
(reduction)Tj
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(in)Tj
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(NADPH)Tj
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(concentration)Tj
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(was)Tj
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(evaluated)Tj
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(10)Tj
1.1678 0 Td
(minutes)Tj
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(in)Tj
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(a)Tj
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(spectropho-)Tj
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(340)Tj
1.6497 0 Td
(nm)Tj
1.5874 0 Td
([)Tj
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(25)Tj
0 g
(].)Tj
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(reductase)Tj
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(\(GR\))Tj
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(activity.)Tj
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(\(GSSG\))Tj
3.2825 0 Td
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(nicotinamide)Tj
5.5502 0 Td
(adenine)Tj
3.3902 0 Td
(dinucleotide)Tj
5.21 0 Td
(phosphate)Tj
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(\(NADPH\))Tj
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1.0148 0 Td
(substrates.)Tj
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-34.9849 -1.2983 Td
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(phosphate)Tj
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(buffer)Tj
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(\(200)Tj
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(mM,)Tj
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(pH)Tj
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(7.5\),)Tj
-33.6809 -1.2982 Td
(EDTA)Tj
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(\(6.3)Tj
1.7405 0 Td
(mM\),)Tj
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(GSSG)Tj
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(\(1)Tj
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(mM\),)Tj
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(mM\))Tj
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(the)Tj
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(samples)Tj
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(25)Tj
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1.7801 0 Td
(consumption)Tj
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-36.1867 -1.2983 Td
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(340)Tj
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(nm)Tj
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(10)Tj
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(minutes.)Tj
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(GR)Tj
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(unit)Tj
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(one)Tj
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1.7008 0 Td
[()]TJ
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(mol)Tj
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-34.3669 -1.3039 Td
(GSSG)Tj
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(minute.)Tj
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1.7858 0 Td
(specific)Tj
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(activity)Tj
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(calculated)Tj
4.2066 0 Td
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(U/mg)Tj
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-24.8597 -1.2983 Td
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9.0425 0 Td
(dehydrogenase)Tj
6.4402 0 Td
(\(G6PDH\))Tj
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(activity.)Tj
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4.1216 0 Td
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(activity)Tj
3.1181 0 Td
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1.0148 0 Td
(glucose-6-phos-)Tj
-30.9994 -1.2983 Td
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(dehydrogenase)Tj
6.2135 0 Td
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(monitored)Tj
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(the)Tj
1.4683 0 Td
(production)Tj
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(of)Tj
1.0092 0 Td
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3.7076 0 Td
(with)Tj
1.9955 0 Td
(a)Tj
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(consequent)Tj
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(increase)Tj
-34.3442 -1.3039 Td
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(absorbance)Tj
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0.9524 0 Td
(340nm.)Tj
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(samples)Tj
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(incubated)Tj
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1.9955 0 Td
(Tris-HCl)Tj
3.8607 0 Td
(buffer)Tj
2.6249 0 Td
(\(100mM,)Tj
3.9231 0 Td
(pH)Tj
1.5024 0 Td
(7.5\),)Tj
-35.3364 -1.2982 Td
(magnesium)Tj
4.8869 0 Td
(chloride)Tj
3.5149 0 Td
(\(MgCl)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 310.0535 289.7574 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 313.6251 291.4015 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(,)Tj
0.4478 0 Td
(2)Tj
0.6917 0 Td
(M\),)Tj
1.6781 0 Td
(NADP)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 368.9574 295.8236 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(+)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 375.5338 291.4015 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\(0.5)Tj
1.7347 0 Td
(mM\))Tj
2.2734 0 Td
(and)Tj
1.7291 0 Td
(glucose-6-phosphate)Tj
8.4642 0 Td
(\(1mM\).)Tj
3.3165 0 Td
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