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Jonghyuk Park, Takahiro Masaki, Yoshihiro Mezaki, Hiroshi Yokoyama, Mariko Nakamura, Haruka Maehashi, Takahiko J. Fujimi, Sabine S. Gouraud, Keisuke Nagatsuma, Madoka Nakagomi, Naofumi Kimura, Tomokazu Matsuura
Alpha-1 antichymotrypsin is involved in astrocyte injury in concert with arginine-vasopressin during the development of acute hepatic encephalopathy
www.plosone.org
www.plosone.org
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-35.3308 -1.2983 Td
(temperature-controlled)Tj
9.5753 0 Td
(room)Tj
2.4265 0 Td
(and)Tj
1.7291 0 Td
(housed)Tj
3.0954 0 Td
(in)Tj
1.0375 0 Td
(individual)Tj
4.2859 0 Td
(cages.)Tj
2.5512 0 Td
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1.3379 0 Td
(minimize)Tj
4.0479 0 Td
(potential)Tj
3.7587 0 Td
(pain)Tj
1.9899 0 Td
(and)Tj
-35.8353 -1.3039 Td
(distress)Tj
3.1917 0 Td
(of)Tj
1.0148 0 Td
(mini-pigs,)Tj
4.3087 0 Td
(the)Tj
1.4683 0 Td
(mini-pigs)Tj
4.0819 0 Td
(were)Tj
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(anesthetized)Tj
5.1647 0 Td
(by)Tj
1.1792 0 Td
(inhalation)Tj
4.2973 0 Td
(of)Tj
1.0148 0 Td
(34%)Tj
2.4492 0 Td
(isoflurane)Tj
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-34.4917 -1.2982 Td
(the)Tj
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(administration)Tj
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(hepatic)Tj
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(euthanized)Tj
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2.0012 0 Td
(an)Tj
1.2019 0 Td
(overdose)Tj
-33.2388 -1.2983 Td
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(isoflurane.)Tj
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(liver)Tj
1.9842 0 Td
(injury)Tj
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(mini-pigs)Tj
4.0819 0 Td
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1.7007 0 Td
(induced)Tj
3.4696 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.652 0 Td
(combination)Tj
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(of)Tj
/F11 1 Tf
1.0148 0 Td
()Tj
/F7 1 Tf
(-amanitin)Tj
-31.9349 -1.2983 Td
(\(Calbiochem,)Tj
5.6012 0 Td
(Darmstadt,)Tj
4.7281 0 Td
(Germany\))Tj
4.3257 0 Td
(at)Tj
0.9524 0 Td
(a)Tj
0.6576 0 Td
(dose)Tj
2.0353 0 Td
(of)Tj
1.0148 0 Td
(0.05)Tj
1.8709 0 Td
(mg/kg)Tj
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1.7291 0 Td
(lipopolysaccharide)Tj
7.6762 0 Td
(\(LPS;)Tj
-33.3919 -1.3039 Td
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2.9083 0 Td
(St.)Tj
1.2189 0 Td
(Louis,)Tj
2.6418 0 Td
(MO,)Tj
2.0863 0 Td
(USA\))Tj
2.4548 0 Td
(at)Tj
0.9524 0 Td
(1)Tj
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0.6974 0 Td
[()]TJ
/F7 1 Tf
(g/kg,)Tj
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(dissolved)Tj
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1.0374 0 Td
(10)Tj
1.1679 0 Td
(ml)Tj
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(saline,)Tj
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(injected)Tj
3.3902 0 Td
(via)Tj
1.372 0 Td
(the)Tj
1.474 0 Td
(splenic)Tj
-33.0347 -1.2983 Td
(vein.)Tj
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2.2847 0 Td
(the)Tj
1.4683 0 Td
(administration)Tj
6.1738 0 Td
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(the)Tj
1.4684 0 Td
(toxins,)Tj
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(mini-pigs)Tj
4.0818 0 Td
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2.126 0 Td
(monitored)Tj
4.4901 0 Td
(every)Tj
2.347 0 Td
(one-hour.)Tj
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-34.6901 -1.2982 Td
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(electrocardiographic)Tj
8.4529 0 Td
(electrodes)Tj
4.2235 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(chest)Tj
2.2564 0 Td
(wall)Tj
1.8312 0 Td
(and)Tj
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(girdled)Tj
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(the)Tj
1.4684 0 Td
(animal)Tj
2.9707 0 Td
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1.9955 0 Td
(a)Tj
0.652 0 Td
(belt)Tj
-35.0813 -1.3039 Td
(incorporating)Tj
5.7429 0 Td
(a)Tj
0.6576 0 Td
(transducer)Tj
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(to)Tj
1.0261 0 Td
(detect)Tj
2.6136 0 Td
(respiratory)Tj
4.5977 0 Td
(movement.)Tj
4.7395 0 Td
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(a)Tj
0.652 0 Td
(catheter)Tj
3.4242 0 Td
(cannulated)Tj
4.6318 0 Td
(in)Tj
1.0318 0 Td
(a)Tj
0.6576 0 Td
(cervical)Tj
3.2598 0 Td
(artery)Tj
2.5739 0 Td
(at)Tj
0.9524 0 Td
(three)Tj
2.2677 0 Td
(times)Tj
2.3868 0 Td
(points:)Tj
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(the)Tj
1.4684 0 Td
(administration)Tj
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(of)Tj
1.0147 0 Td
(hepatic)Tj
3.1125 0 Td
(toxins)Tj
2.6645 0 Td
(\(mushroom-derived)Tj
/F11 1 Tf
8.3452 0 Td
()Tj
/F7 1 Tf
(-amanitin)Tj
4.7621 0 Td
(and)Tj
1.7292 0 Td
(LPS\),)Tj
2.3584 0 Td
(when)Tj
2.4037 0 Td
(cerebral)Tj
3.4186 0 Td
(edema)Tj
2.846 0 Td
(occurred)Tj
-32.6549 -1.3039 Td
(following)Tj
3.9798 0 Td
(acute)Tj
2.3244 0 Td
(hepatic)Tj
3.1124 0 Td
(encephalopathy,)Tj
6.7294 0 Td
(and)Tj
1.7291 0 Td
(after)Tj
2.0353 0 Td
(treatment)Tj
4.1385 0 Td
(with)Tj
1.9956 0 Td
(EC)Tj
1.4513 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
1.4684 0 Td
(BAL)Tj
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(for)Tj
1.389 0 Td
(6)Tj
0.6973 0 Td
(hours.)Tj
-34.2536 -1.2982 Td
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(sample)Tj
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(a)Tj
0.6576 0 Td
(plasma)Tj
3.0331 0 Td
(separator)Tj
3.9344 0 Td
(tube.)Tj
-28.2725 -1.2983 Td
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1.7858 0 Td
(protocol)Tj
3.5943 0 Td
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(animal)Tj
2.9707 0 Td
(experiments)Tj
5.142 0 Td
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(created)Tj
3.1238 0 Td
(based)Tj
2.4718 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
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(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
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-33.1254 -1.3039 Td
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3.7133 0 Td
(of)Tj
1.0148 0 Td
(Animal)Tj
3.2201 0 Td
(Experiments)Tj
5.2838 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(Science)Tj
3.2031 0 Td
(Council)Tj
3.4016 0 Td
(of)Tj
1.0148 0 Td
(Japan)Tj
2.4831 0 Td
(and)Tj
1.7348 0 Td
(approved)Tj
3.9685 0 Td
(by)Tj
1.1849 0 Td
(the)Tj
1.4683 0 Td
(Institu-)Tj
-34.1799 -1.2983 Td
(tional)Tj
2.5171 0 Td
(Animal)Tj
3.2201 0 Td
(Care)Tj
2.1147 0 Td
(and)Tj
1.7291 0 Td
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1.7405 0 Td
(Committee)Tj
4.7395 0 Td
(of)Tj
1.0147 0 Td
(the)Tj
1.4741 0 Td
(Jikei)Tj
2.0012 0 Td
(University.)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 302.1165 cm
BT
/F0 1 Tf
1 TL
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5.3244 0 Td
(of)Tj
1.0441 0 Td
(plasma)Tj
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(two-dimension)Tj
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(gel)Tj
1.5307 0 Td
(electrophoresis)Tj
ET
Q
q
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1 J
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BT
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(\(IEF\))Tj
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(Uppsala,)Tj
3.6907 0 Td
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(12)Tj
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(h,)Tj
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(200)Tj
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(1)Tj
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(500)Tj
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(1000)Tj
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(1)Tj
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(9)Tj
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(sodium-dodecyl)Tj
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(sis)Tj
1.2075 0 Td
(\(SDS-PAGE\))Tj
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(running)Tj
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(buffer)Tj
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(disequilibrium)Tj
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(the)Tj
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(second)Tj
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(dimen-)Tj
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(sion)Tj
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(electrophoresis,)Tj
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(the)Tj
1.4683 0 Td
(equilibrated)Tj
5.0173 0 Td
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(SDS)Tj
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(II)Tj
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(electrophoresis)Tj
6.2475 0 Td
(kit)Tj
1.2813 0 Td
(\(Bio-Rad,)Tj
4.0762 0 Td
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3.9911 0 Td
(CA,)Tj
1.7915 0 Td
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Q
q
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1 J
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BT
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1 TL
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(LC-MS/MS)Tj
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(mass)Tj
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Q
q
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BT
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2.8516 0 Td
(chromatography-tan)Tj
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(particles,)Tj
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(200)Tj
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[()]TJ
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5.8223 0 Td
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(were)Tj
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(acetonitrile)Tj
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(2%)Tj
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(aceto-)Tj
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(and)Tj
1.7291 0 Td
(0.1%)Tj
2.1544 0 Td
(formic)Tj
2.897 0 Td
(acid;)Tj
2.0976 0 Td
(buffer;)Tj
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(B-)Tj
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(90%)Tj
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(acetonitrile)Tj
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(and)Tj
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(0.1%)Tj
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(formic)Tj
2.897 0 Td
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(with)Tj
1.9956 0 Td
(a)Tj
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(flow)Tj
1.9446 0 Td
(rate)Tj
ET
Q
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1 J
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36 737.1 l
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576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
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W* n
1 j
1 J
0 w
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BT
/F0 1 Tf
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(of)Tj
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(alpha-1)Tj
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(antichym)Tj
3.9331 0 Td
(otrypsin)Tj
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(astrocyte)Tj
4.2165 0 Td
(injury)Tj
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(hepatic)Tj
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h
f*
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BT
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(PLOS)Tj
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(ONE)Tj
2.3669 0 Td
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(https://doi.or)Tj
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