Transformation products elucidation of forchlorfenuron in postharvest kiwifruit by time-of-flight mass spectrometry

Forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea, FCF) is a plant growth regulator, being extensively used for increasing kiwifruit size. The toxicological properties of its may persist in their transformation products (TPs) or even higher toxicity than FCF. TPs elucidation of FCF in postharvest kiwifruit (Actinidia chinensis, Chinese gooseberry) by the liquid chromatography ionization hybrid ion trap and time-of-flight mass spectrometry (LC-ESI-IT-TOF/MS) in positive mode was the objective of the present study. Fifteen days after full bloom, kiwifruits were dipped for 5s with high dosage FCF solution (60 mg/L), so that sufficient peaks could be detected. The chemical structure of unknown TPs was analyzed in combination of functions of LCMS-IT-TOF, such as high-accurate MSn, formula predictor, metabolite structural analysis software MetID Solution, profiling solution metabolomics software, and neutral loss, characteristic isotopic patterns of chlorine, the fragmentation pattern and retention time of standard substances, nitrogen rule, chemical components of kiwifruit. Total 17 TPs were detected via comparisons of their accurate MSn data of commercial analytical standards and synthesized standards with high purity, such as 4-amino-2-chloropyridine, phenylurea, 2-hydroxy-FCF, 1-(2-chloro-6-((3, 4, 5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl) oxy) pyridin-4-yl)-3-phenylurea, 1, 3-bis (2-chloropyridin-4-yl) urea, 1,3-diphenylurea, 1-(2-chloropyridin-4-yl)urea, FCF-2-O-β-D-glucoside, and so on. The major transformation pathways of FCF in kiwifruit were biochemical and photochemical cleavage pathway. The experimental results indicate that LCMS-IT-TOF is powerful and effective tool for identification of FCF TPs.


Introduction
FCF is a plant growth regulator, being extensively used for increasing fruit size and weight in watermelon, kiwifruit, grape and apple [1][2][3][4]. In China, the incidence of FCF had caused an a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 adverse impact on the kiwifruit industry. Residue analysis and dissipation of FCF in fruit and vegetable had already been studied by high-performance liquid chromatography with ultraviolet detection (HPLC/UV) [5], liquid chromatography-tandem mass spectrometry [6][7][8], liquid chromatography time-of-flight mass spectrometry (LC/TOF-MS) [9]. An enzyme-linked immunosorbent assay (ELISA) had been developed for the determination of FCF in fruit [10][11][12]. Evaluation of the new activity of FCF in the product was reported by Australian pesticides and veterinary medicines authority. FCF has low oral toxicity in rats with an LD50 of 4940 mg/kg bw in male rats and 4899 mg/kg bw in females [13]. In recent years, some researches indicated that TPs of agrochemistry could be more toxic than the parent molecule [14,15]. So FCF and its TPs could be a potential health hazard. However, the detection of TPs was a difficult work to do, due to the lack of standards [16]. Currently, the time-of-flight (TOF) mass analyzers had been used to detect TPs of agrochemistry [17][18][19][20][21].
LCMS-IT-TOF (Shimadzu) is a type of mass spectrometer that combines ion trap and TOF (time-of-flight) technologies. The instrument possesses some advantages and function, such as high accuracy MS n , Formula Predictions software, MetID solution software [22]. LCMS-IT-TOF/MS had been successfully applied to the identification of metabolites of FR429 [23], herbal homologs, strictosamide, phencynonate [24,25],. In this paper, LCMS-IT-TOF had been employed to identify the major TPs of FCF in postharvest kiwifruit.

Material and methods
Ethics statement I state clearly that no specific permissions were required for these locations/activities, because this site is a normal kiwifruit orchard without protected wildlife and protected area of land or sea. The authors confirm that the field studies did not involve endangered or protected species. The study was approved by Shaanxi Bairui Kiwi Fruit Research Institute Co. Ltd.

Plant material
The trial was carried out in Xi'an city, Shaanxi province, China, in 2013~2015, in a 7-year-old kiwifruit orchard of Hayward from Shaanxi Bairui Kiwi Fruit Research Institute Co. Ltd (340 3 0 N, 108˚25 0 E). The vines were spaced 5×4 m, trained to the T-bar trellis system. Twenty vines were selected, uniform in vegetative and reproductive characteristics. Fifteen days after full bloom, all fruitlets of fifteen vines were dipped for 5 s with FCF (ethanol solvent, 60 mg/L), sufficient peaks could be detected in high dosage, while the fruit of the other five vines were dipped with water only (blank). Storage time of postharvest kiwifruit is one month.

Sample preparation
Extraction of FCF and TPs from kiwifruit samples was carried out according to the QuE-ChERS method [26,27]: (1) FCF treated samples were placed in a blender and chopped. (2) 300 g of thoroughly homogenized sample was placed into a 1000 mL glass beaker, mixed thoroughly with 300 mL of acetonitrile and 120 g anhydrous magnesium sulfate, and 30 g anhydrous sodium acetate using ultrasonic-assisted extraction for 40 min. The homogenate was allowed to settle and the supernatant was filtered through a filter paper into a 1000 mL rotary-evaporation flask. The solid residue was washed twice with 60 mL of acetonitrile. A rotary evaporator set at 50˚C and 250 mbar was used to evaporate the extract to less than 5 mL, and then, the extract was passed to a graduated conical tube (15 mL) and evaporated to dryness at 50˚C. The sample was reconstituted in water: acetonitrile (1:1) and filtered through a 0.22 μm filter [28].

Chromatographic conditions
LC analysis was conducted on a LC-20AD system from Shimadzu (Kyoto, Japan). Chromatographic separation was achieved on a Shim-pack XR ODS column (2.2μm 3.0 mm×75 mm from Shimadzu). The mobile phase (0.2 mL min -1 ) consisted of solvent A (0.1% formic acid in acetonitrile) and B (0.1% formic acid in water). Elution condition was performed with a linear gradient 95~30% B from 0 to 5 min, 30~0% B from 5 to10 min, retained until 1 min then quickly returned to initial 95% B and maintained for 20 min for column balance [28].
The identification process of FCF and its TPs involves four procedural steps: (1) sample preparation (2) elucidated the fragment pattern of FCF and its 6 TPs standard substance. (3) The chemical structure of unknown TPs was analyzed in combination of functions of LCMS-IT-TOF, such as high-accurate MS n , formula predictor, metabolite structural analysis software MetID Solution, profiling solution metabolomics software, and neutral loss, characteristic isotopic patterns of chlorine, the fragmentation pattern and retention time of standard substances, nitrogen rule, chemical components of kiwifruit. Some suspected TPs peaks which had the same MS n and fragment pattern with FCF and its 6 TPs standard substance were found by full scan analysis in positive mode. (4) concluded their chemistry structure.

Results and discussion
Fragmentation mass spectra for standard substances of (possible) transformation products Forchlorfenuron (M0) and 6 TPs (10 μg mL -1 ) standard substance prepared respectively in methanol was used for the fragmentation pattern study in positive ion mode.  Table 1 shows analytical information of the 6 TPs standard substance, including retention time, elemental compositions, and mass error in positive ion mode. Fig 2 shows the proposed fragmentation pattern of M0 and 6 TPs standard substance [28].

Identification of forchlorfenuron transformation products in postharvest kiwifruit
One month after kiwifruit posthavest, 17 TPs were deduced by analysis of LCMS-IT-TOF.   TP2 (see Fig 4A, Table 2) gave rise to the protonated molecule at m/z 172.0280 with a retention time of 4.085 min, and it was calculated as C 6 H 6 ClN 3 O (error, 4.7 ppm) by the Formula Predictor software. The MS 2 products ions at m/z 155.0012 (error, 3.2 ppm), m/z 129.0219 (error, 3.9 ppm) were characteristic fragment ions of M0, and the product ion at m/z 155.0012 was formed through neutral losing of NH 3 . Thus, TP2 was identified as 1-(2-chloropyridin-4-yl)urea. The proposed structure and the fragment pathway for TP2 are shown in Fig 4A. TP3 (see Fig 4B, Table 2 ). Thus, TP5, TP7, TP11 were elucidated by the hydroxylation product of TP14. According to the polar order, we could infer hydroxy position on the benzene ring, TP5, TP7, TP11 is respectively 1-(4-hydroxy-phenyl)-3-phenylurea, 1-(3-hydroxy-phenyl)-3-phenylurea, 1-(2-hydroxy-phenyl)-3-phenylurea. The proposed chemical structure and the fragment pathway for TP5, TP7, TP11 are respectively shown in Fig 4C. TP6 showed the protonated molecular ion [M+H] + at m/z 426.1087 (see Fig 4B, Table 2 . Thus, we concluded TP6 was the glycosylation product of M0. As can be seen in Fig 4B, the MS 1 of TP6 yield  Fig 4B. TP8, TP10 (see Fig 4D, Fig 4E, Table 2) and TP12 exhibited respectively the protonated molecular ion  Transformation products elucidation of forchlorfenuron in kiwifruit could be preliminary concluded as the hydroxylation product of M0. TP8, TP10 and TP12 shared the same MS 2 fragment ions at m/z 155.0007, m/z 129.0214, m/z 110.0600 with ST5/ ST6. The retention time error between TP8 and ST5, TP10 and ST6 were respectively 0min, 0.003min. Therefore, TP8 was identified as ST5, TP10 was identified as ST6. As ST5/ST6 was respectively 4-hydroxy-FCF and 3-hydroxy-FCF, therefore, TP12 was identified as 2-hydroxy-FCF. The chemical structure and the fragment pathway of TP12 had been concluded and shown in Fig 4E. The protonated molecular ion of TP9 (see Fig 4D, Table 2, m/z 426.1045, error -4.2 ppm), retention time 5.610 min, yielded three MS 2 ions at m/z 307.0664(error, -8.8 ppm), m/z 264.0560 (error, 9.8 ppm), m/z 145.0169 (error, 4.1 ppm). As can be seen in Table 1 TP14 (see Fig 4F, Table 2) showed the protonated molecular ion [M+H] + at m/z 291.0636, was 43.0051 Da higher than that of the molecular ion of M0 (m/z 248.0585) and the ratio of the relative intensities of the principal isotopes, A to A+2 was approximately 3:1, suggesting the presence of a chlorine atom (the natural abundance of 35 Cl: 37 Cl is 3:1). The neutral loss of 43.0051 Da was calculated as HNCO by the Formula Predictor software. The MS 2 (m/z 137.0711, error, 1.5 ppm) was formed through neutral losing of m/z 153.9932 (C 6 H 3 ClN 2 O). Thus, we concluded the fragment ion at m/z 137.0711 was the combination of HNCO and aniline, and its combination position was located on amino of aniline. Further analysis showed the fragment ion at m/z 137.0711 was formed by the reaction of aniline and amino acid in kiwifruit sample. Thus, TP14 was identified as 1-carbamoyl-3-(2-chloropyridin-4-yl) -1-phenylurea. The chemical structure and the fragment pathway of TP14 are shown in Fig 4F. TP15 (see Fig 4G, Table 2, m/z 213.1021, error, -0.5 ppm) was calculated as C 13 H 12 N 2 O by the Formula Predictor software according to the accurate mass measurement. As can be seen in Fig 4G, the molecular ion of TP15 showed the major product ions at m/z 94.0651 (error, 0 ppm). Thus, TP15 was identified as 1, 3-diphenylurea, and its chemical structures and fragment pattern were shown in Fig 4F. TP16 (see Fig 4G, Table 2) was detected at the retention time of 7.086 min and gave a protonated molecule ion at m/z 262.0744 (error, 0.8 ppm), and there were two characteristic fragment ions at m/z 154.9999, m/z 129.0215 of M0. The protonated molecule at m/z 262.0744 was Transformation products elucidation of forchlorfenuron in kiwifruit 14.0157 Da (CH 2 ) higher than that of M0, and the product ion of m/z 108.0813 was 14.0162 Da (CH 2 ) higher than that of phenylamine, and also the MS 2 ion at m/z 108.0813 was generated through the neutral loss of C 6 H 3 ClN 2 O. Thus, we concluded the position of CH 2 was located at the benzene ring, and it could be 1-(2-chloropyridin-4-yl)-3-(o-tolyl) urea or 1-(2-chloropyridin-4-yl)-3-(m-tolyl) urea or 1-(2-chloropyridin-4-yl)-3-(p-tolyl) urea. Its accurate structure need to be further identified by the reference standards. We tentatively concluded the proposed structure and the fragment pathway of TP16 were shown in Fig 4G. TP17 (see Fig 4F,   Transformation products elucidation of forchlorfenuron in kiwifruit -4.4 ppm), m/z 213.1036 (error, 6.6 ppm) and the fragment ion at m/z 213.1036 could lead to one MS 3 product ions at m/z 94.0658. The products ions at m/z 248.0574, m/z 213.1036 were calculated as respectively C 12 H 10 ClN 3 O (M0), C 13 H 12 N 2 O (TP15) by Formula Predictor software. Thus, TP17 was a reaction product of M0 and TP15 and identified as 3-(2-chloropyridin-4-yl)-1-phenyl-1-(phenylcarbamoyl)urea. The chemical structures of TP17 and its fragment pattern were shown in Fig 4F. Degradation and metabolism pathway of forchlorfenuron in kiwifruit According to the deduced structure formulas of the 17 TPs (Fig 5), the degradation and metabolism pathway of FCF in kiwifruit were proposed and are shown in Fig 6. The present study showed that 17 TPs could be found and determined, several of which were discovered for the first time. These data indicate some metabolic pathways of FCF, such as, FCF was cleaved to TP1, TP2, TP4, its hydroxylated product is TP8, TP10, TP12, furthermore, its glycosylation product is TP3, TP6, TP9.

Conclusions
Total 17 TPs of FCF in postharvest kiwifruit were detected by LCMS-IT-TOF, and the main transformation pathways were hydroxylation, glycosylation, methylation, cleavage, oxidation, reduction, and so on. The experimental results indicate that LCMS-IT-TOF is powerful and effective tool for identification of plant growth regulation TPs.