Parental alcohol use and risk of behavioral and emotional problems in offspring

Objective The majority of studies that have examined parental alcohol use and offspring outcomes have either focused on exposure in the antenatal period or from clinical populations. This study sought to examine proximal and distal associations between parental alcohol use and offspring conduct problems and depressive symptoms in a population birth cohort. Methods We used prospective data from a large UK based population cohort (ALSPAC) to investigate the association between parental alcohol use, measured in units, (assessed at ages 4 and 12 years) with childhood conduct trajectories, (assessed on six occasions from 4 to 13.5 years, n = 6,927), and adolescent depressive symptoms (assessed on four occasions from ~13 to ~18 years, n = 5,539). Heavy drinking was defined as ≥21 units per week in mothers and partners who drank 4+ units daily. Results We found little evidence to support a dose response association between parental alcohol use and offspring outcomes. For example, we found insufficient evidence to support an association between maternal alcohol use at age 4 years and childhood conduct problems (childhood limited: OR = 1.00, 95% CI = .99, 1.01; adolescent onset: OR = 0.99, 95% CI = .98, 1.00; and early-onset persistent: OR = 0.99, 95% CI = .98, 1.00) per 1-unit change in maternal alcohol use compared to those with low levels of conduct problems. We also found insufficient evidence to support an association between maternal alcohol use at age 4 years and adolescent depressive symptoms (intercept: b = .001, 95% CI = -.01, .01, and slope: b = .003, 95% CI = -.03, .03) per 1-unit change in maternal alcohol use. Results remained consistent across amount of alcohol consumed (i.e., number of alcohol units or heavy alcohol use), parent (maternal self-reports or maternal reports of partner’s alcohol use), and timing of alcohol use (assessed at age 4 or age 12 years). Conclusions There is no support for an association between parental alcohol use during childhood and conduct and emotional problems during childhood or adolescence.


Introduction
An estimated 2.6 million children in the UK (22%) live with a parent who drinks hazardously [1].Parental alcohol use can have a profound impact on their child's development, with children showing increased emotional and behavioral difficulties [2].Alcohol epidemiology has traditionally focused on alcohol exposure during the antenatal period e.g., [3][4][5][6][7][8][9], and has largely focused on substance use and educational outcomes [10].Less is known about the influence of parental alcohol use during childhood and the impact on offspring mental health outcomes.The studies that have examined the association between parental alcohol use during childhood and offspring mental health outcomes have mainly focused on samples involving heavy alcohol use or alcoholic parents and outcomes in very early childhood [11][12][13][14].However, there is a need to examine the association between light to moderate alcohol use and child outcomes across childhood and adolescence.
All studies focusing on alcohol use suffer from some methodological considerations.For example, under-reporting of alcohol consumption is a challenge in observational studies [15] and is particularly common in studies based on pregnancy samples [16].Furthermore, the paradoxical protective effects of antenatal parental alcohol use found in some studies [9,17,18] are most likely explained by 1) misclassification of the exposure or outcome, 2) residual confounding, or 3) small sample size [6,19].One possible explanation for these protective effects in the literature is that offspring outcomes were measured at only one occasion; that is, some of the behaviors being examined may not have been evident at the time of measurement, as there is evidence that problems vary across time [20].
One previous study [21], using this sample (Avon Longitudinal Study of Parents and Children, ALSPAC), examined alcohol use in both the antenatal and postnatal periods, found that maternal alcohol consumption was positively associated with offspring externalizing behaviors.However, these effects were largely evident for maternal alcohol consumption during pregnancy, rather than the postnatal period, suggesting evidence for fetal exposure to the intrauterine effects to alcohol [8].
In this study, we extend previous work, that has used the ALSPAC sample, to examine parental alcohol use in the antenatal period e.g., [6,7,[21][22][23] by focusing on the association between parental alcohol use during childhood and longitudinal trajectories of youth mental health problems.Specifically, the aim is to use a more flexible trajectory approach, allowing for developmental variability, to examine the impact of both proximal and distal measures of parental alcohol use on youth behavioral and emotional problems.

Participants and procedure
We used data from the Avon Longitudinal Study of Parents and Children (ALSPAC), which recruited 14,541 pregnant mothers who resided in the former Avon Health Authority in the southwest of England, and had an estimated date of delivery between April 1, 1991 and December 31, 1992.Of the 13,978 offspring alive at one year, a small number of participants withdrew from the study (n = 24), leaving a starting sample of 13,954.ALSPAC provide a range of options for withdrawal of consent e.g., participation break, withdrawal from direct participation, withdrawal from study-maintaining permission to use existing data.For a detailed overview of our study population including attrition at the different measurement occasions (S1a-S1c Fig) .Detailed information about ALSPAC is available online www.bris.ac.uk/alspac and in the cohort profiles [24,25].A fully searchable data dictionary is available on the study's website (www.bris.ac.uk/alspac/researchers/data-access/data-dictionary/).Ethical approval for the study was obtained from the ALSPAC Ethics and Law Committee and the Local Research Ethics Committees.

Measures
Parental alcohol consumption.We used a combination of maternal reports of their own and their partner's alcohol consumption.In assessing maternal alcohol use, mothers completed a postal questionnaire at 47 months and 12 years 1 month asking about their daily alcohol consumption.From here, we refer to these measurement occasions as age 4 and 12 years respectively.Reponses were converted into units e.g., ½ pint of 'beer, larger, or cider' reflects 1 unit, one glass of wine reflects 2 units, one pub measure 'spirit' reflects 1 unit, 'other alcoholic drinks' reflects 1 unit, and 'low alcoholic drinks' reflects 0.5 of a unit; 'sherry and others' reflects 1 unit; and 'ready mix drinks' reflects 1.5 units.One drink was equivalent to one UK unit of alcohol, corresponding to 8 grams of alcohol.For our analyses maternal alcohol exposure was defined as a total sum score reflecting the number of alcohol units consumed in one week.Heavy alcohol use was defined as drinking 21 units per week, reflecting on average 3 units per day, considerably above the weekly governmental guidelines of 14 units of alcohol [26].
We also used a maternal-reported measure of partner alcohol consumption collected at the same measurement occasions.Partner's frequency of drinking 4+ units was assessed using the following question: 'How many days in the past month do you think he had the equivalent of 2 pints of beer, 4 glasses of wine or 4 pub measures of spirit?' Response options were: none, 1-2 days, 3-4 days, 5-10 days, more than 10 days, and every day, treated as a continuous measure.Heavy alcohol use was defined as drinking 4+ units daily.
Offspring outcome measures.Maternal reports of youth conduct problems were measured using the conduct subscale of the Strengths and Difficulties Questionnaire (SDQ) [27] assessed on six occasions at approximately 4, 7, 8, 10, 12, and 13.5 years.The SDQ is a wellvalidated measure with a large amount of peer-reviewed literature detailing its psychometric properties [28,29].Of the sample of 9,600 mothers who had information on alcohol use, 72.1% (n = 6,927/9,600) of offspring had information on at least four measures of conduct problems.There was strong evidence of a relationship between sociodemographic variables and trajectories of conduct problems (S1 Table ).
Heterogeneity in childhood conduct problems is well established, see [30,31].From this framework, Barker and Maughan [32] derived four developmental trajectories of conduct problems throughout childhood using latent class growth analysis which have been used in a number of subsequent analyses e.g., [33][34][35].Sum scores were dichotomised at the threshold of four or more at each measurement occasion.Binary cut-offs were established based on national norms established for children in England and Wales [36].The four-class model comprised of children with low involvement with conduct problems (Low, 64% of the sample, 48.9% boys), childhood limited (CL, 15% of the sample, 54.1% boys), adolescent onset (AO, 12% of the sample, 49.7% boys), and early onset persistent (EOP, 9% of the sample, 56.8% boys).Focusing on these conduct trajectory groupings allows for developmentally sensitive patterns of conduct problems to be examined, rather than the approach of focusing on one time point, which assigns similarity to children who display similar levels of problems at this time point but importantly display different developmental trajectories.For our purposes, we examined trajectories of conduct problems for the sample as a whole as the previous work by Barker and Maughan found that gender-invariant models provided adequate fit to the data.
Adolescent depressive symptoms were measured using the self-report Mood and Feelings Questionnaire-short form (MFQ) [37] assessed on four occasions at 12y 10m, 13y 10m, 16y 6m, and 17y 10m.Of the sample of 9,600 mothers who had information on alcohol use, 57.7% (n = 5,539) of offspring provided at least two measures of depressive symptoms.There was strong evidence of a relationship between sociodemographic variables and missing data on depressive symptoms (S2 Table ).Previously, Edwards and colleagues [38] using a dimensional approach, found strong evidence of variation in symptom levels at baseline and also symptom growth for males and females respectively.
For the purposes of our study, we estimated latent growth models for males and females separately using the knownclass option in Mplus as the development of depressive symptoms has previously been shown to differ across gender during early adolescence e.g., [39].As well as maximizing power, this approach produces a single covariate estimate by constraining the association between the covariate with the intercept and slope to be equal and by constraining the variances to be equal.The residual variances were freely estimated across gender, but constrained within time.
Potential confounding variables.A range of measures were considered to be potential confounders of the parental alcohol-offspring mental health relationship.These comprised of established risk factors for conduct problems and depression outcomes for which we felt the assumption of a causal predictive relationship with parental alcohol use could be justified.Measures of social economic position (SEP) were recorded by maternal self-report questionnaires during pregnancy.These included maternal age at delivery, parity (1, 2, 3 children), socioeconomic position (grouped into four categories: 1) unskilled/semiskilled manual; 2) skilled manual/nonmanual; 3) managerial/technical; and 4) professional), maternal education (<O level: indicating no qualification; O level: indicating completion of school examinations at age 16; and >O level: indicating completion of college or university education at or after age 18), maternal smoking during first trimester in pregnancy (yes/no), housing tenure (mortgaged, subsidised renting, private renting), income (measured in quintiles), and maternal depressive symptoms measured using the Edinburgh Postnatal Depression Scale [40] at 32 weeks gestation

Statistical methods
Childhood conduct problems.The association between parental alcohol consumption at age 4 years (using linear and binary exposures) and developmental trajectories of offspring conduct problems across all 6 timepoints (i.e., EOP, AO, CL, and Low as the reference group) were examined using multinomial logistic regression.In estimating class membership, simulation work [41][42][43] demonstrated that the standard three-step modal class approach can introduce bias affecting the strength of the association between the latent classes and observed covariates.For this reason, we used the 'auxiliary (r3step)' command in Mplus, which allows for bias-adjusted estimates and has been shown to lead to less-biased estimates than the traditional three-step methods [44].This approach allows for the most likely class membership to be obtained from the posterior probabilities along with classification uncertainty; the most likely class membership variables can then be analyzed to include covariates while accounting for the measurement error in classification [45].

Adolescent depressive symptoms.
Offspring depressive symptom scores across all four time points were used.The intercept factor loadings were all fixed at one and the slope factor loadings were fixed to reflect the amount of time in months between assessments with baseline at zero.After the unconditional models were estimated, a series of four separate conditional latent growth models (LGM), which included maternal and partner alcohol consumption at age 4 years (i.e., distal exposure) and at age 12 years (i.e., proximal exposure) as predictors of depressive symptoms intercept and slope factors, at each time point were estimated.

Missing data
9,600 mothers provided self-report information on alcohol use at age 4 years.Of these, 8,139/9,600 (84.8%) provided information on their partners alcohol use at the same time period.At age 12 years, 5,931/9,600 (61.8%) mothers provided self-report information on alcohol use.Of these, 5,535/9,600 (57.7%) provided information on their partner's alcohol use at the same time period.Since all of the confounders were assessed in early pregnancy, there was minimal missing data (e.g., parental social class had the most amount of missing data: 817/9,600 (8.5%)).Associations between background socioeconomic variables and offspring conduct problems and depressive symptoms are presented in S3 and S4 Tables.
Using complete case analysis and not taking missing data into account can result in biased estimates [46].As previously described [32,38], missing data on the outcome measures were dealt with using full information maximum likelihood (FIML).Once the outcome models were derived, inverse probability weighting (IPW) [47] was used as a sampling weight to investigate the possible influence of selective participation on our estimates of association between parental alcohol and offspring conduct problems and depressive symptoms, respectively.In this way, estimates were weighted to account for probabilities of nonresponse for the mental health outcomes.The process of weighting, using IPW, allows us to give more weights to individuals who have similar prenatal characteristics to those of individuals who are likely to subsequently be lost to the study.
Within the sample with complete data on maternal alcohol, IPW was used to derive logistic regression models predicting having complete conduct problem trajectory data (n = 6,927).Response rates differed according to: maternal age, offspring gender, grandmother having a history of severe depression, maternal alcohol use in pregnancy, intentional pregnancy, damp/ mould on walls in the house, marital status, and car ownership (S5 Table ).The Hosmer-Lemeshow test was used to assess model fit and included respondents were then weighted by the inverse of this probability.The reciprocal of the predicted probabilities from these models were used as sampling weights to adjust the regression models of interest.IPW was performed using Stata 13.The weighted models are presented as our main findings; the unweighted results are presented in S6a-S7b Tables.
This procedure was repeated for the depressive symptoms model; logistic regression model predicting having at least two measures of depressive symptoms (n = 5,539).Of these, n = 1,624 had information on three measures; while n = 2,422 had information on all four depression measures.Again there was evidence of an association between all of the variables with loss to follow-up (results available on request).Weights ranged from 1.2 to 18.4 for conduct problems models and from 1.3 to 21.4 for depression models.Due to the potential for extreme weighted values adversely influencing subsequent analyses, larger weights were trimmed to 10.All models were analysed in Mplus v7.11 using the maximum likelihood estimator [48].

Descriptive results
Available information for maternal alcohol use and partner's frequency of drinking 4+ units at ages 4 and 12 years and conduct problems and depressive symptoms is reported in Table 1.There was good agreement between maternal and partners reports of partners frequency of drinking 4+ units at 4 years ( = .87).
Table 2 shows the association between the parental alcohol measures.We found strong evidence of an association between alcohol measures for each parent within and across time, with stronger associations within person and within time.
Group-based trajectories of conduct problems.Table 3 presents the associations between parental alcohol use at age 4 years (using linear and binary terms) and the four conduct trajectory classes.The pattern of results did not change after controlling for confounding variables.Overall, using the weighted estimates, we found insufficient evidence of an association between parental alcohol use at age 4 years and group-based trajectories of conduct problems across childhood.There was a suggestion of an association between maternal alcohol use examining heavy parental alcohol use and conduct problems limited to childhood compared to low conduct problems (OR = 1.56, 95% CI = 1.05, 2.34).However, this association was attenuated when models were adjusted for confounding variables (OR = 1.40, 95% CI = 0.93, 2.11).We found little evidence for any further associations.
Trajectories of adolescent depressive symptoms.Table 4 presents the associations between parental alcohol use at ages 4 and 12 years (using linear and binary terms) and adolescent depressive symptoms before and after adjusting for potential confounding variables.There was weak evidence of an association between partner alcohol use and baseline adolescent depressive symptoms after adjusting for confounding variables (b = -.065,95% CI = -.13,-.00, p = .05).We found little evidence of an association between heavy parental alcohol use (using binary alcohol exposures) for either unadjusted or adjusted models.Results highlighting the pattern of depressive symptoms across adolescence for males and females grouped by heavy and non-heavy maternal alcohol use at age 4 years are displayed in the Supplementary Material (S2 Fig) .Finally, as a sensitivity set of analyses, antenatal alcohol use at age 18 weeks gestation was included (S8a-S9b Tables).The pattern of results remain unchanged.

Discussion
Based on a large prospective birth cohort, we found insufficient evidence of an association between parental alcohol, measured using proximal and distal measures, and trajectories of childhood conduct problems or adolescent depressive symptoms.Although there was a suggestion of some weak associations using the unadjusted models, these associations were attenuated when models were adjusted for a number of confounding variables.The present study should be considered in light of a number of limitations.As with any longitudinal study, data were not complete on exposures, outcomes, and confounders for the whole cohort.Although we cannot rule out the possibility that exclusion of mothers without complete information might have biased our findings for reported alcohol use, there was minimal change to the models when differential dropout was accounted for using inverse probability weighting.Furthermore, a previous study found evidence that differential attrition does not affect estimates of risk for behavioural disorders in ALSPAC [49].Second, self-reported alcohol information is generally underreported, however there is evidence that reports outside of the antenatal period may be more accurate than prenatal reports given that alcohol use in pregnancy is underreported to a greater extent [16].Third, although the assessment of parental alcohol use was different in terms of quantity and frequency of alcohol consumed, they do capture a broad account of parental drinking practices.Fourth, the use of partner reports of their own alcohol usage would have been optimal, however maternal reports of their partners alcohol use were used because of 1) the rate of attrition in partner reporting, especially for the assessment at age 12 years, and 2) the very good inter-rater agreement between parental reports of partners drinking practices.Fifth, it is possible that the association between parental alcohol use and offspring outcomes is only evident at the extreme end of alcohol consumption.In examining this possibility, we found no evidence to support an association between heavy  alcohol use (using binary measures of maternal and partner's alcohol use) and offspring conduct and emotional problems.Sixth, one of our outcomes (group-based trajectories of conduct problems) was based on maternal reports which raises the possibility of measurement bias due to differential misclassification of the outcome (i.e., conduct problems) across exposure groups (alcohol use versus no alcohol use).Finally, although the SDQ is used as a screening instrument for mental health problems in epidemiological research rather than providing a clinical diagnoses, a recent systematic review [50] reported that parent-reported versions of the conduct problems subscale had a sensitivity of 0.75 and a specificity of 0.91 for the detections of conduct disorder.

Comparison with previous studies
Unlike the few studies that have examined the association between parental alcohol use during childhood and offspring emotional and behavioral outcomes [51,52], we found little evidence in support of this association.The contrast in findings could be due to a number of possibilities.First, our study used a population sample in contrast to the majority of previous studies that have used clinical samples.For example, the studies by Hussong and colleagues defined their alcohol measure as a lifetime diagnosis of alcoholism, while in our study, alcohol use largely reflected more light to moderate drinking practices.The use of clinical samples may lead to an overestimation of the association between parental alcohol use and youth outcomes due to the selection of more severely impaired parents, therefore limiting generalizability [53].Furthermore, large population samples are better powered to detect associations compared to smaller clinical samples, as the use of relatively small samples may indicate that bias could be impacting on the findings.Second, our study utilized more robust outcome measures (i.e., group-based trajectory modelling and latent growth models), capturing rich longitudinal information over childhood and adolescence which is important for following markedly different developmental trajectories.
Third, being able to examine maternal and paternal alcohol use separately is important as previous studies have suggested that maternal alcohol use may be more hazardous to offspring mental health problems than paternal alcohol use [53][54][55].On this note, there was no evidence of an association between parental alcohol consumption and offspring conduct problems, as these symptoms show stronger relations compared to emotional symptoms [55].Finally, we found little evidence of an association between distal or proximal effects of parental alcohol use on youth mental health problems which have previously been shown in children of alcoholics to be largely related to distal factors for both externalizing and internalizing symptoms [51,52].
Previous studies examining varying levels of antenatal alcohol use (i.e., light, moderate, heavy, and alcohol dependence) have demonstrated adverse effects on a range of offspring outcomes [56]; while others have demonstrated no associations [17,18,57,58].Although this study was primarily addressing whether parental alcohol use during childhood was associated with later offspring emotional and behavioural difficulties, it is possible that maternal drinking in pregnancy could impact on this association through an intrauterine mechanism, although the findings are mixed.To address this concern, maternal drinking in pregnancy was included as a potential confounder.The overall pattern of results remained unchanged, indicating that light to moderate maternal drinking in pregnancy did not impact on this association.Furthermore, studies that have incorporated a genetic approach to the understanding of the association between maternal alcohol use in the antenatal period and offspring outcomes, using a Mendelian Randomization (MR) design, have on the whole demonstrated adverse associations of moderate maternal drinking in pregnancy and offspring outcomes [5,9,59].An MR approach was not used in these analyses as the same genetic instruments would be related to the prenatal and postnatal periods and as a result it would not be possible to separate out these effects.

Implications and conclusions
We found insufficient evidence of an association between parental alcohol use and offspring conduct problems or depressive symptoms-further contributing to the inconsistency of the evidence base on the importance of parental alcohol use during childhood as an influence and risk for offspring mental health outcomes across childhood and adolescence.As our study focused largely on light to moderate parental alcohol use, it cannot be ruled out that findings would differ when examining much higher levels (e.g., alcohol dependence).partner's alcohol consumption; 2 Univariable multinomial logistic regression models; 3 Multinomial logistic regression models adjusted for maternal age at delivery, parity, Social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation; CL: childhood limited, AO: adolescent onset, EOP: early onset persistent, the Low conduct problems class was used as the reference group.

Table 4 .
Parental alcohol use (assessed at age 4 and 12 years using linear alcohol measures) and adolescent depressive symptoms.
(B).Heavy parental alcohol consumption (assessed at age 4 years using binary alcohol measures) and childhood conduct problem trajectoriesunweighted estimates.Note:1 Maternal reports of partner's alcohol consumption; CL: childhood limited, AO: adolescent onset, EOP: early onset persistent, the Low conduct problems class was used as the reference group.2Models adjusted for maternal age at delivery, parity, social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation.(DOCX) S7 Table.(A) Parental alcohol use (assessed at age 4 and 12 years using linear alcohol measures) and adolescent depressive symptoms-unweighted estimates.Note. 1 Maternal reports of partner's alcohol consumption; 2 Univariable linear regression models; 3 Models adjusted for maternal age at delivery, parity, social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation.(B).Heavy parental alcohol use (assessed at ages 4 and 12 years using binary alcohol measures) and adolescent offspring depressive symptomsunweighted estimates.Note: 1 Maternal reports of partner's alcohol consumption; 2 Univariable linear regression models; 3 Models adjusted for maternal age at delivery, parity, social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation.(DOCX) S8 Table.(A) Childhood conduct problem trajectories and parental alcohol consumption-unweighted estimates (low group-reference group).Note: 1 Maternal reports of partner's alcohol consumption; Model 1 adjusted for maternal age at delivery, parity, social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation; Model 2 further adjusted for maternal alcohol use at 18 weeks gestation; CL: childhood limited, AO: adolescent onset, EOP: early onset persistent, the Low conduct problems class was used as the reference group.(B).Heavy parental alcohol consumption (assessed at age 4 years using binary alcohol measures) and childhood conduct problem trajectories-unweighted estimates.Note: 1 Maternal reports of partner's alcohol consumption; Model 1 adjusted for maternal age at delivery, parity, social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation; Model 2 further adjusted for maternal alcohol use at 18 weeks gestation; CL: childhood limited, AO: adolescent onset, EOP: early onset persistent, the Low conduct problems class was used as the reference group.(DOCX) S9 Table.(A) Parental alcohol use (assessed at age 4 and 12 years using linear alcohol measures) and adolescent depressive symptoms-unweighted estimates-complete cases.Note. 1 Maternal reports of partner's alcohol consumption; Model 1 adjusted for maternal age at delivery, parity, social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation; Model 2 further adjusted for maternal alcohol use at 18 weeks gestation.(B).Heavy parental alcohol use (assessed at ages 4 and 12 years using binary alcohol measures) and adolescent offspring depressive symptoms-unweighted estimates.Note. 1 Maternal reports of partner's alcohol consumption; Model 1 adjusted for maternal age at delivery, parity, social economic position, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation; Model 2 further adjusted for maternal alcohol use at 18 weeks gestation.(DOCX)

Table 1 . Descriptive information for maternal and partner alcohol use for both conduct problems and depressive symptoms. Conduct problems trajectories sample Depressive symptoms trajectories sample
1 Maternal reports of partner's alcohol consumption https://doi.org/10.1371/journal.pone.0178862.t001

Table 3 . Parental alcohol consumption (assessed at age 4 years using linear and binary measures) and childhood conduct problem trajectories. Unadjusted models Adjusted models 2 N CL OR (95% CI) AO OR (95% CI) EOP OR (95% CI) p N CL OR (95% CI) AO OR (95% CI) EOP OR (95% CI) p Linear alcohol measure
2Multinomial logistic regression models adjusted for maternal age at delivery, parity, SEP, maternal education, maternal smoking during first trimester in pregnancy, housing tenure, income, and maternal depressive symptoms at 32 weeks gestation; CL: childhood limited, AO: adolescent onset, EOP: early onset persistent, the Low conduct problems class was used as the reference group.https://doi.org/10.1371/journal.pone.0178862.t003