Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase

C5a drives airway constriction and inflammation during the effector phase of allergic asthma, mainly through the activation of C5a receptor 1 (C5aR1). Yet, C5aR1 expression on myeloid and lymphoid cells during the allergic effector phase is ill-defined. Recently, we generated and characterized a floxed green fluorescent protein (GFP)-C5aR1 knock-in mouse. Here, we used this reporter strain to monitor C5aR1 expression in airway, pulmonary and lymph node cells during the effector phase of OVA-driven allergic asthma. C5aR1 reporter and wildtype mice developed a similar allergic phenotype with comparable airway resistance, mucus production, eosinophilic/neutrophilic airway inflammation and Th2/Th17 cytokine production. During the allergic effector phase, C5aR1 expression increased in lung tissue eosinophils but decreased in airway and pulmonary macrophages as well as in pulmonary CD11b+ conventional dendritic cells (cDCs) and monocyte-derived DCs (moDCs). Surprisingly, expression in neutrophils was not affected. Of note, moDCs but not CD11b+ cDCs from mediastinal lymph nodes (mLN) expressed less C5aR1 than DCs residing in the lung after OVA challenge. Finally, neither CD103+ cDCs nor cells of the lymphoid lineage such as Th2 or Th17-differentiated CD4+ T cells, B cells or type 2 innate lymphoid cells (ILC2) expressed C5aR1 under allergic conditions. Our findings demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and mLN of mice, suggesting that the C5a/C5aR1 axis controls airway constriction and inflammation through activation of myeloid cells in all three compartments in an experimental model of allergic asthma.


Introduction
Allergic asthma is one of the most prevalent diseases of the western world. It develops in genetically susceptible individuals as a chronic inflammatory disorder of the upper airways leading a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 OVA-Alum (200μg/2mg) on days 0 and 7 and challenged intratracheally (i.t.) with 1.5% OVA (in 50 μl PBS) on days 14,16,18, and 20. On day 21 (24 h after the last i.t. administration), AHR was determined and tissue samples were harvested for further analysis.
Collection of Bronchoalveolar Lavage (BAL) fluid and differential cell counting BAL fluid samples were obtained by cannulating the trachea, injecting 1 ml of ice-cold PBS, and by subsequently aspirating the BAL fluid. After red blood cell lysis, BAL fluid cells were washed once in PBS and counted using a Neubauer chamber (Assistant, Germany). Frequencies of BAL fluid cells were determined by flow cytometry. Cell numbers were calculated using cell specific frequency of total and total cell counts/ml.

Lung histology
Lung histological staining, detection and quantification of mucus cell content were done as described previously [24]. Slides were stained either with hematoxylin and eosin (H/E), or periodic acid-Schiff (PAS). PAS positive and PAS negative airways were counted by light microscopy and the percentages of PAS positive airways were calculated to quantify mucus production.

Statistical analysis
Statistical analysis was performed using the GraphPad Prism version 5 (GraphPad Software, Inc.). Normal distribution of data was tested using the Kolmogorov-Smirnov and D'Agostino-Pearson tests, some after log transformation. When groups were normally distributed, statistical differences between two groups were analyzed by unpaired t test. Comparisons involving multiple groups were first analyzed by ANOVA followed by Tukey's test. When groups were not normally distributed, they were analyzed using a Mann-Whitney U (two groups), or an ANOVA on ranks (mutiple groups) followed by a Dunn's multiple comparison test. A p value < 0.05 was considered as Ã or § significant, p <0.01 as ÃÃ or § § significant and p <0.001 as ÃÃÃ significant.

Results
Characterization of the allergic phenotype and C5aR1 expression in WT and GFP-C5aR1 flox/flox mice state conditions. Further, we observed C5aR1 expression in BAL macrophages [15]. Here, we monitored C5aR1-expression in myeloid and lymphoid cells in a model of OVA-driven allergic asthma (S1 Fig). Twenty-four hours after the final challenge, we determined airway resistance and airway inflammation. OVA-immunized WT and GFP-C5aR1 flox/flox mice suffered from a significant increase in AHR in comparison to PBS-treated controls ( Fig 1A). GFP-C5aR1 flox/flox mice showed a higher airway resistance than WT animals at low methacholine concentrations. In contrast, both strains reached a similar maximum at 50 mg/ml methacholine. Upon PBS treatment, macrophage numbers were high in the BAL of WT and GFP-C5aR1 flox/flox mice ( Fig 1C). Upon OVA stimulation, we observed a strong recruitment of neutrophils, eosinophils and lymphocytes into the bronchoalveolar compartment in both, WT and GFP-C5aR1 flox/flox mice. The most prominent cell populations were neutrophils and eosinophils. T cell numbers were much lower ( Fig 1C).
Then, by measuring the GFP signal as a surrogate marker for C5aR1 expression, we determined C5aR1 expression in immune cells present in the airways after OVA-treatment. We found a strong C5aR1 expression in eosinophils, macrophages and neutrophils, but no C5aR1 expression in CD4 + T cells ( Fig 1D). The increased production of mucus by goblet cells is another feature of allergic asthma (Fig 1E and 1F). Compared to PBS treated control mice, WT and GFP-C5aR1 flox/flox mice showed a significant increase in the frequency of mucus-positive airways in response to OVA challenge ( Fig 1E). We found no differences between WT and GFP-C5aR1 flox/flox allergic asthma groups.
Collectively, our data show that WT and GFP-C5aR1 flox/flox mice develop a similar asthmatic phenotype with comparable airway inflammation, mucus production but somewhat higher airway sensitivity of the reporter mice towards low methacholine concentrations. Further, myeloid but not lymphoid-derived cells express C5aR1 in the airways.
Similar accumulation of myeloid cells in lung tissue of WT and GFP-C5aR1 flox/flox mice Next, we performed histological examination of lungs from WT and GFP-C5aR1 flox/flox mice. We observed a strong recruitment of immune cells around blood vessels and airways (Fig 2A). To further characterize the composition of the infiltrate, lung cells were analyzed by flow cytometry (Fig 2B and 2D). Among the inflammatory cells, neutrophils were dominant in OVA-treated mice ( Fig 2C). Also, the number of eosinophils and macrophages increased significantly in WT and GFP-C5aR1 flox/flox mice. We identified three pulmonary DC subsets in the lung ( Fig 2D) [13]. At steady state, CD11b + cDCs, CD103 + cDCs and a minor population of moDCs were present ( Fig 2E). In response to OVA, the numbers of CD103 + , CD11b + cDCs and moDCs markedly increased (Fig 2E). Within the CD11b + DC population about 20-30% were CD11b + cDCs, 70-80% were moDCs in WT and GFP-C5aR1 flox/flox mice after OVA challenge. Overall, the accumulation of cDCs and moDCs was similar in both strains.

Accumulation and differentiation of lymphoid cells in lung tissue of WT and GFP-C5aR1 flox/flox mice
The number of CD4 + T cells increased significantly in WT and GFP-C5aR1 flox/flox mice under asthmatic conditions (Fig 3A and 3B). However, total CD4 + T cell numbers in GFP-C5aR1 flox/flox mice were lower than in WT mice. CD44 + CD62 + effector T cells markedly increased in WT and C5aR1 reporter mice. Of note, we found that naïve CD44 -CD62L + T cells increased significantly in WT but remained at the same level in C5aR1 flox/flox mice in response to OVA-treatment. Next, we determined the differentiation pattern of pulmonary effector T cells into Th1, Th2, Th17 and Treg subtypes in sorted CD44 + CD62effector T-cells. We found 100-times higher levels of mRNA encoding for FoxP3, GATA3, RORγT than those encoding for TBX21 (TBet) with no differences between cells from WT or GFP-C5aR1 flox/flox mice ( Fig 3C). These findings demonstrate a clear Th2/Th17 commitment of most effector T cells. In addition, a strong population of Treg cells was present ( Fig 3C). In line with the dominance of Th2/ Th17 cells, we observed a high and similar abundance of IL-13 and IL-17A transcripts in CD44 + C62Leffector T cells from WT and GFP-C5aR1 flox/flox mice ( Fig 3D).
Taken together, our data demonstrate that the accumulation but not the differentiation of total CD4 + T cells is impaired in GFP-C5aR1 flox/flox mice as compared with WT mice. In contrast to CD4 + T cells, the accumulation of ILC2 is not affected in GFP-C5aR1 flox/flox mice.

Comparison of C5aR1 expression in myeloid and lymphoid cells from the alveolar, pulmonary and mLN compartments
Next, we compared C5aR1 expression in myeloid cells from BAL, lung and mLNs in response to PBS treatment or OVA immunization. In agreement with our previous findings [15], eosinophils, macrophages and neutrophils homogeneously expressed C5aR1 in the PBS group whereas CD103 + cDCs lacked C5aR1 expression ( Fig 4A, S2 Fig). The C5aR1 expression was strongest in alveolar macrophages and decreased in lung cells in the order macrophages > neutrophils = moDCs >> CD11b + cDCs > eosinophils ( Fig 4A; Table 1). Interestingly, macrophages from the alveolar compartment expressed significantly higher GFP levels than lung macrophages in response to PBS treatment ( Table 1). As we found no eosinophils or neutrophils in the BAL of PBS-treated mice (Fig 1C), we could only compare GFP expression in eosinophils or neutrophils from the alveolar and lung tissue compartments following OVAimmunization. While neutrophils from BAL or lung tissue expressed C5aR1 to a similar degree, eosinophils from BAL expressed significantly higher levels of C5aR1 than eosinophils from lung tissue ( Table 2). C5aR1 expression in BAL alveolar macrophages and in lung macrophages was significantly down-regulated upon OVA-immunization, (Fig 4A and 4B, Table 1). In contrast to eosinophils, neutrophils and macrophages, we noticed that only a small subpopulation of pulmonary CD11b + cDCs expressed C5aR1 (Fig 4C). While the majority of moDCs expressed C5aR1, some cells stained GFP negative (Fig 4C). During the allergic effector phase, n = 9-16 per group. (B) Gating strategy for the BAL fluid cell analysis. Cells were identified by flow cytometry using different markers to identify macrophages (SiglecF + autofluorescence + ), eosinophils (SiglecF + autofluorescence -) neutrophils (SiglecF -Ly6G + CD4 -), and T cells (SiglecF -Ly6G -CD4 + ). (C) Total and differential cell counts in BAL fluid of PBS-treated or OVA-immunized WT or GFP-C5aR1 flox/flox animals. Values shown are the mean ± SEM; n = 9-17 per group. (D) GFP/C5aR1 expression in eosinophils, macrophages, neutrophils and CD4 + T cells from BAL fluid of GFP-C5aR1 reporter mice in response to OVA; grey histogram: WT controls. (E) Histological examination of mucus production in the airways of PBS-treated or OVA-immunized WT or GFP-C5aR1 flox/flox animals. Sections were stained with PAS for mucus production (original magnification x 200). (F) Frequency of PAS-positive bronchi in PBS-treated or OVA-immunized WT or GFP-C5aR1 flox/flox animals. Mucus producing airways are plotted relative to all analysed airways. Values shown are the mean ± SEM; n = 4-8 per group. * indicates significant differences between the PBS and OVA treatment groups; § indicates significant differences between OVA-treated WT and GFP-C5aR1 flox/flox mice. * or § p < 0.05, ** p < 0.01, *** p <0.001. C5aR1 expression was significantly reduced in pulmonary CD11b + cDCs and moDCs as compared with PBS-treated controls ( Fig 4D, Table 1).
Importantly, lung tissue eosinophils significantly increased their GFP levels in response to OVA treatment (Fig 4B; left panel; Table 1). In contrast, the inflammatory allergic conditions neither induced C5aR1 expression in CD103 + cDCs (S2A Fig) nor did they modulate the expression of C5aR1 in neutrophils (S2B Fig). Interestingly, C5aR1 expression in CD11b + cDCs from mLNs was lower than that in CD11b + cDCs from lung tissue in the PBS-treated group (Table 1). In contrast, the already markedly decreased expression of C5aR1 in pulmonary CD11b + cDCs in response to OVA challenge (as compared to PBS treatment) did not change in CD11 + cDCs that were present in the mLNs ( Table 2). Of note, the C5aR1 expression of moDCs in mLNs was lower than that of moDCs in the lung ( Table 2).
Recent studies reported that CD4 + T cells express C5aR1 at steady state and after activation [21,27]. Previously, we found no GFP signal or C5aR1 mRNA expression in naïve or in vitro activated T cells from the spleen [15]. In line with these findings, total and effector CD4 + T cells from the lung were negative for the GFP signal in response to PBS treatment or under OVA-driven allergic conditions (Fig 4E). Further pulmonary B cells and ILC2 cells stained C5aR1 negative upon allergic conditions (Fig 4E).
Taken together, our data demonstrate that pulmonary C5aR1 expression is restricted to cells of the myeloid lineage under steady state and allergic conditions. Further, in an established allergic environment, C5aR1 is differentially regulated in macrophages and DCs vs. eosinophils. While C5aR1 is strongly upregulated in eosinophils it is significantly downregulated in macrophages, CD11b + cDCs and moDCs.

C5aR1 surface expression correlates with GFP expression in eosinophils, macrophages and CD11b + cDCs
To assess whether the GFP + cells also express C5aR1 at the cell surface, we counterstained them with a C5aR1-specific antibody. As shown by flow cytometry, naïve lung eosinophils and alveolar macrophages stained homogeneously positive for C5aR1. The specificity was confirmed by comparing the C5aR1 signal in WT and C5aR1 -/mice (S3A Fig). Similarly, the C5aR1 expression followed the GFP expression in lung eosinophils and macrophages ( Fig 5A). As observed for the GFP signal (Fig 4A), the surface expression of C5aR1 decreased markedly in response to OVA-immunization but increased significantly on the surface of eosinophils (Fig 5A).
In accordance with our previous findings [15], the pulmonary DC subsets, CD11b + cDCs and moDCs were positive for C5aR1, whereas CD103 + cDCs did not express C5aR1 (S3B Fig). Similar to the GFP signal, only a small fraction of CD11b + cDCs expressed C5aR1 on the cell surface ( Fig 5B). In response to OVA treatment, C5aR1 surface expression was abrogated in CD11b + cDCs (Fig 5B). The majority of moDCs expressed C5aR1 at the cell surface. In contrast to the GFP expression, we found no clear reduction of the C5aR1 surface signal upon eosinophils (SiglecF + CD11c -), macrophages (SiglecF + CD11c + ) or neutrophils (Ly6G + SiglecF -) in lung tissue. (C) Differential cell counts of PBS-treated or OVA-immunized WT or GFP-C5aR1 flox/flox animals. Values shown are the mean ± SEM; n = 9-17 per group. (D) Gating strategy to identify DC subsets in lung tissue. Data shown represent the pulmonary cell composition of OVA-treated mice. Cells were first gated on SiglecFcells. Then lineage negative cells were excluded. Subsequently DCs were identified as CD11c + MHCII + cells. These cells were further subdivided into CD103 + CD11b − or CD103 − CD11b + cDCs. Within the latter population, we identified CD11b + CD64 -cDCs and CD11b + CD64 + moDCs. (E) DC counts in lung cell suspensions of PBS-treated or OVAimmunized WT or GFP-C5aR1 flox/flox animals. Values shown are the mean ± SEM; n = 7-18 per group. * indicates significant differences between PBS or OVA-treated groups. * p < 0.05, ** p < 0.01, *** p <0.001.  OVA challenge (Fig 5B). Thus, except for moDCs, our data demonstrate that the GFP expression goes along with C5aR1 surface expression.

Accumulation and C5aR1 expression of DCs and CD4 + T cells in mLNs from WT or GFP-C5aR1 flox/flox mice
In search for mechanisms underlying the decreased accumulation of CD4 + T cells in the lung of GFP-C5aR1 flox/flox as compared with WT mice, we evaluated the homing of DCs to and the development of effector T cells in the mLNs. We found very low DC numbers in PBS-treated animals. In contrast, we observed a clear increase in CD11b + cDCs and CD103 + cDCs in WT and GFP-C5aR1 flox/flox mice after OVA challenge, although the number of CD103 + cDCs was much lower than the CD11b + cDC numbers (Fig 6A). Also, moDC number increased in response to OVA-immunization. Of note, the number of moDCs in WT was slightly higher than that in GFP-C5aR1 flox/flox mice.
T cell numbers in the mLNs were slightly higher after OVA challenge than after PBS treatment. The numbers of total, CD44 + CD62Leffector or CD44 -CD62L + naive T cells cells in WT and GFP-C5aR1 flox/flox mice mLN were similar (Fig 6B). In line with our observation in T cells from the alveolar and the pulmonary tissue compartments, we found no C5aR1 expression in CD4 + T cells from mLNs ( Fig 6C). In summary, cDC and CD4 + T cell numbers were not altered in GFP-C5aR1 flox/flox mice, whereas moDC numbers were slightly decreased. Given that moDCs can prime Th2 effector cell differentiation [13], the decreased number of moDCs in GFP-C5aR1 flox/flox mice may explain, at least in part, the decreased pulmonary CD4 + T cell number (Fig 3B).

Discussion
C5a regulates the development of experimental allergic asthma during allergen sensitization and the effector phase through activation of its two cognate receptors C5aR1 and C5aR2 [5]. The C5aR1 expression and activation on defined innate and adaptive immune cells in established allergic asthma is ill-defined. Recently, we demonstrated the expression of C5aR1 in pulmonary eosinophils, neutrophils, macrophages, CD11b + cDCs, and moDCs using a floxed GFP-C5aR1 knock-in mouse [15]. Here, we used this mouse strain as a tool to monitor the expression of C5aR1 in innate and adaptive immune cells in the alveolar space, the lung and the draining lymph nodes during the allergic effector phase.
First, we assessed the allergic phenotype in GFP-C5aR1 flox/flox and their WT littermates in an experimental OVA/Alum-driven allergic asthma model. Both strains developed a similar allergic phenotype with comparable airway inflammation and mucus production. However, GFP-C5aR1 flox/flox mice were more sensitive to low concentrations of methacholine than their WT controls. These data suggest a change in the contractility of airway smooth muscle cells (ASMs) in the GFP-C5aR1 flox/flox animals resulting in airway narrowing at low doses of the bronchoconstrictor. C5aR1 expression in C5aR1 flox/flox mice is somewhat lower than in wildtype mice [15]. Further, C5 or C5aR1 targeting is associated with increased airway hyperresponsiveness [3,7]. Thus, the slightly decreased C5aR1 expression in C5aR1 flox/flox mice may account for the increased sensitivity and airway narrowing at low doses of methacholine.
In the lungs of WT and GFP-C5aR1 flox/flox mice, we observed a marked increase in macrophages, eosinophils and neutrophils as well as CD11b + and CD103 + cDCs and moDCs after the final OVA challenge. The increase in lung cDCs was associated with increased homing of CD11b + and CD103 + cDCs to the mLN in WT and C5aR1 reporter mice. Interestingly, the  C5aR1 expression in the allergic asthma effector phase numbers of CD11b + cDCs were about 10-fold higher than that of CD103 + cDCs in both strains. In addition, we observed migration of moDCs to the mLN, which was in the range of CD103 + cDCs. Of note, the number of moDCs from GFP-C5aR1 flox/flox mice that had migrated to the mLNs 24h after the final OVA administration was significantly higher than that of WT mice. A dominant migration of CD11b + cDCs has been described in HDM-driven experimental asthma [13]. However, in contrast to our findings, moDCs were absent 24h after the final allergen challenge and peaked at day 3. The individual role of CD103 + and CD11b + cDCs in triggering Th2 immune is still controversial. Important roles for both cDC subtypes have been reported [13,28,29]. CD11b + cDCs take up most of the allergen and activate T cells by several costimulatory molecules including CD86 and OX40L or Dectin-2, which are important for Th2/Th17 differentiation and survival [13,30,31]. Further, moDCs can prime Th2 immunity [13]. Thus, stronger Th2 priming through the increased number of mLN moDCs in WT mice may account for the higher number of pulmonary CD4 + T cells that we found in the lung of WT mice. CD103 + cDC and moDCs are the main producers of Th2 homing chemokines CCL17 and CCL22 [13,32]. The similar numbers of pulmonary CD103 + cDCs and moDCs in WT and C5aR1 reporter mice indicate that pulmonary CD4 + T cell homing is not affected in C5aR1 reporter mice. In summary, we found that C5aR1 reporter mice exerted a similar allergic phenotype as WT mice with strong AHR, mucus production, airway inflammation and homing of cDCs and moDCs to mLNs. Further, we observed similar inflammation in C5aR1 expression in the allergic asthma effector phase the lung with comparable numbers of eosinophils, macrophages and neutrophils, CD11b + and CD103 + cDCs, moDCs and ILC2, whereas CD4 + T cell numbers were lower than in WT controls.
Our previous findings demonstrated that the C5a/C5aR1 signalling axis is instrumental for the development of the allergic asthma phenotype [3,7]. Previous reports have shown an increase of C5aR1 mRNA in the lung of asthmatic rats [10]. However, the myeloid and/or lymphoid cell types that express the C5aR1 under asthmatic conditions remained unclear. Taking advantage of the C5aR1 reporter mice, we evaluated the C5aR1 expression in myeloid and on lymphoid cells in the airways, the lung and the mLN during the effector phase after the final allergen challenge. Interestingly, C5aR1 expression increased in pulmonary eosinophils. While C5aR1 has been described in eosinophils [33,34] no data exist about its regulation in lung eosinophils under asthmatic conditions. Eosinophils migrate towards C5a, degranulate [35] and produce reactive oxygen species (ROS) [33]. Thus, the increased C5aR1 expression in lung eosinophils suggests that the C5a/C5aR1 axis plays an important role in the activation of tissue eosinophils. Further, we observed a strong C5aR1 expression in eosinophils accumulating in the airways after final OVA exposure, which was even higher than that of lung tissue eosinophils. Importantly, C5a increases the adhesion of eosinophils through upregulated expression of CD11b [36]. Thus, our data indicate that C5a may play an important role in the extravasation of eosinophils from the circulation into the alveolar space through the regulation of adhesion molecules.
In contrast to eosinophils, C5aR1 expression was high in alveolar macrophages under steady state conditions but decreased significantly after OVA immunization. As macrophage numbers in the airways did not change upon OVA, these data suggest a downregulation of C5aR1 in response to on-going C5a generation in the inflammatory environment of established allergic asthma [3,4]. Alternatively, the decrease in C5aR1 surface expression could also result from proteolytic C5aR1 cleavage in response to neutrophil-derived serine proteases as described by van den Berg et al. [37], or via an unknown mechanism involving CRP [38]. However, the decrease in surface C5aR1 parallels the decrease in the GFP signal, suggesting down regulation of C5ar1 mRNA, eventually resulting in decrease of C5aR1 surface expression. Similarly, we observed a decrease in GFP and C5aR1 in tissue-associated macrophages. Recently, C5aR1 has been associated with alveolar macrophage apoptosis in acute lung injury [39]. Further, the C5a/C5aR1 axis contributed to the instability of atherosclerotic lesions by accelerated macrophage apoptosis [40]. Thus, downregulation of C5aR1 on macrophages during the allergic inflammation may limit cell death of alveolar and tissue-associated macrophages to maintain their anti-inflammatory properties [41,42].
Finally, C5aR1 expression was profoundly regulated in CD11b + cDCs and moDCs. First, C5aR1 was strongly downregulated in CD11b + cDCs that migrated from the lung to the draining LNs under steady state conditions. Previous findings suggest that activation of the C5a/ C5aR1 axis on cDCs is important for CD4 + T cell proliferation and differentiation [43,44]. Downregulation of C5aR1 in cDCs trafficking from the lung to draining LNs under steady state conditions may protect from undesired cDC activation. Further, we observed downregulation of the already low C5aR1 expression on pulmonary CD11b + cDCs to almost zero and significant decrease of C5aR1 expression in moDCs after OVA challenge. We and others have previously shown that massive complement occurs in an established allergic environment both in mice [3] and humans [4,45,46] resulting in the C3a and C5a generation. Thus, the high concentrations of anaphylatoxins in the lung may account for anaphylatoxin receptor internalization and downregulation on CD11b + cDCs and moDCs. The already decreased C5aR1 expression in moDCs was further downregulated, when the cells migrated to the mLN. Of note, the GFP expression did not match the C5aR1 surface expression determined by a C5aR1-specific antibody. Loss of GFP fluorescence intensity has been observed under low pH conditions (pH<5) [47] which exist in late endosomes or lysosomes [48] and may account for the drop in the MFI. Indeed, intracellular localization of C5aR1 together with C5aR1 has been found in endosomes of human monocytes [49] and CD4 + T cells [50] suggesting a redistribution of the C5aR1 protein pool in moDCs under inflammatory allergic conditions.
The expression of C5aR1 in murine T cells at steady state or upon activation is still a matter of debate. Different studies reported C5aR1 expression in resting murine CD4 + T cells [21,27,51,52]. In contrast, we and others found no C5aR1 expression in naïve, in vitro or in vivo activated CD4 + T cells, neither at the mRNA nor protein level [15,20]. Here, we demonstrate that Th2/Th17-differentiated CD4 + T cells lack C5aR1 expression during the allergic effector phase. Further, we found no C5aR1 expression in pulmonary B cells and ILC2. Our data confirm that cells from the lymphoid lineage do not express C5aR1, at least not in mice.
In summary, our findings demonstrate a complex regulation of C5aR1 in eosinophils, macrophages CD11b + cDCs and moDCs in the effector phase of allergic asthma. C5aR1 is highly expressed in resident alveolar macrophages as well as neutrophils and eosinophils that migrate into the airways upon allergen challenge. C5aR1 expression on lung tissue macrophages, CD11b + cDCs and moDCs decreases in an established allergic environment but increases in pulmonary eosinophils. The C5aR1 expression in airway or lung tissue neutrophils is high under steady state and allergic conditions. Also, we observed a rather complex pattern of C5aR1 regulation of CD11b + cDCs and moDCs that migrated from the lung into the mLN. In contrast to human CD4 + T cells, we neither found C5aR1 expression under steady state nor under allergic conditions, when they differentiated into Th2 or Th17 effector cells. Also, C5aR1 expression was absent in ILC2 and B cells during the allergic effector phase. Our results suggest an important role of the C5a/C5aR1 axis in eosinophil recruitment and/or activation in the effector phase of experimental allergic asthma. Late-onset eosinophilic asthma is a relatively rare form of asthma with frequent severe exacerbations that requires systemic corticoid treatment [53]. C5aR1 may serve as an alternative target or complementary approach to anti-Th2 cytokine antibodies targeting IL-4, IL-5 or IL-13.