Structural and Behavioral Correlates of HIV Infection among Pregnant Women in a Country with a Highly Generalized HIV Epidemic: A Cross-Sectional Study with a Probability Sample of Antenatal Care Facilities in Swaziland

Introduction HIV disproportionately affects women in Sub-Saharan Africa. Swaziland bears the highest HIV prevalence of 41% among pregnant women in this region. This heightened HIV-epidemic reflects the importance of context-specific interventions. Apart from routine HIV surveillance, studies that examine structural and behavioral factors associated with HIV infection among women may facilitate the revitalization of existing programs and provide insights to inform context-specific HIV prevention interventions. Methods and Findings This cross-sectional study employed a two-stage random cluster sampling in ten antenatal health care facilities in the Hhohho region of Swaziland in August and September 2015. Participants were eligible for the study if they were 18 years or older and had tested for HIV. Self-administered tablet-based questionnaires were used to assess HIV risk factors. Of all eligible pregnant women, 827 (92.4%) participated, out of which 297 (35.9%) were self-reportedly HIV positive. Among structural factors, family function was not significantly associated with self-reported HIV positive status, while lower than high school educational attainment (AOR, 1.65; CI, 1.14–3.38; P = 0.008), and income below minimum wage (AOR, 1.81; CI, 1.09–3.01; P = 0.021) were significantly associated with self-reported HIV positive status. Behavioral factors significantly associated with reporting a positive HIV status included; ≥2 lifetime sexual partners (AOR, 3.16; CI, 2.00–5.00; P<0.001), and ever cohabited (AOR, 2.39; CI, 1.66–3.43; P = 0.00). The most cited reason for having multiple sexual partners was financial gain. HIV/AIDS-related knowledge level was high but not associated to self-reported HIV status (P = 0.319). Conclusions Structural and behavioral factors showed significant association with self-reported HIV infection among pregnant women in Swaziland while HIV/AIDS-related knowledge and family function did not. This suggests that HIV interventions should be reinforced taking into consideration these findings. The findings also suggest the importance of future research sensitive to the Swazi and African sociocultural contexts, especially research for family function.


Introduction
According to the World Health Organization, HIV/AIDS-related death is the worldwide leading cause of death among women of reproductive age [1]. Globally, 15% of women living with HIV in 2013 were of age 15-24 years, of whom 80% lived in Sub-Saharan Africa (SSA) [2]. Within the SSA region, the burden of HIV among women of age 15-49 years varies considerably; 7.6% in Kenya (2014) [3], 16.9% in Namibia (2014) [4], 19.0% in South Africa (2015) [5] and Swaziland bears the highest HIV prevalence of 38.8% (2011) [6]. The majority (62%) of all new infections in Swaziland occur among women [7]. HIV prevalence in Swaziland is higher among women aged 18-49 years (38.8%) compared to their male counterparts of the same age group (23.1%), and particularly high among women aged 30-34 years old, 54% compared to 37% in men of the similar age group [6]. Although there have been reports of a decline in HIV prevalence among women in southern Africa [8,9], at best, the epidemic in Swaziland seems to have only stabilized [6,10].

Young women's vulnerability to HIV
Young women's vulnerability to HIV could be attributed to several factors. First, families have great influence among young women. Studies show that lack of parental monitoring, poor parent-child communication, and low cohesion among family members are associated with increased HIV risky sexual behavior [11][12][13][14][15][16]. Second, research has shown a strong link between HIV/AIDS and poverty. Frequently, poverty drives girls and women to exchange sex for food or basic amenities and cause a day-to-day existence dominated by immediate survival needs and indifference to high HIV risk sexual behaviors [17][18][19][20][21]. Furthermore, most young women growing up in economically deprived families have little access to schooling and few future prospects, therefore, find themselves coerced into sexual activity with older working men for survival [22,23]. Third, behavioral factors such as; early sexual debut [24][25][26][27], inconsistent condom use [28][29][30], multiple sexual partnerships (concurrent and serial) [31][32][33], poor sexual-decision making under the influence of alcohol [34][35][36] and others, have shown to increase HIV vulnerability among young women. Lastly, there is extensive literature identifying biological factors putting women at particularly higher risk of HIV compared to men. As pointed out by Ramjee and colleague "women have a greater vaginal mucosal surface area exposed to pathogens and infectious fluid for longer periods during sexual intercourse, and that young women are particularly at higher risk due to cervical ectopy which facilitates greater exposure of target cells to trauma and pathogenesis in the vagina [9]".

Review of previous studies in Swaziland
In Swaziland, there are limited studies specifically focusing on HIV vulnerability among young women. Existing research has focused on men who have sex with men [37], sex workers [38], in-school youth [39] and the general population [40,41]. Studies on young women have either been qualitative [42][43][44] -which are suited to exploring risks at in-depth levels, but, fall short in quantifying risk-or sentinel surveillance studies using face-to-face interviews [45] which afford less privacy and anonymity and thus likely increase motivational bias [46,47]. This study aims to investigate HIV risk factors between HIV negative and HIV positive young women to provide empirical evidence specific to Swaziland. To achieve this goal, we studied pregnant women attending antenatal care since the median age at first birth is 19.8 years [48] and the majority (98.5%) of pregnant women access antenatal care services in Swaziland [49]. We used simple two-stage cluster sampling and self-administered computer-assisted data collection technique to overcome shortcomings of prior research.

Ethical considerations
This study was conducted according to the ethical principles outlined in the Declaration of Helsinki. The research protocol was approved by the Kyoto University Faculty of Medicine and Graduate School of Medicine, Ethics Committee, Japan (R0073) and the Swaziland Scientific and Ethics Committee, Swaziland (MH/599C/FWA00015267/IRB0009688). All participants signed a written informed consent. One USD (1$) was offered to each participant as compensation for taking part in our study.

Study setting
Swaziland, is a small land-locked country situated in Southern Africa. Its area is approximately 17 364 km 2 with an estimated population of 1 287 050 (2015), of which about 76% reside in rural areas [50]. Swaziland is divided into four administrative regions: Hhohho, Manzini, Lubombo, and Shiselweni region. The number of health facilities that provide antenatal care services to pregnant women per region is as follows: 52 in Hhohho, 63 in Manzini, 38 in Lubombo and 30 in Shiselweni [50]. In the Hhohho region, of all the facilities which offer antenatal health services, 78.8% are public and the rest (21.2%) are private facilities. The Hhohho region was selected as our study setting since it has the highest generalized HIV prevalence in the country; 27.8% in Hhohho, 21.9% in Shiselweni, 20.7% in Manzini, and 20.5% in Lubombo [40].

Participants
Our study targeted pregnant women who were !18 years old, had tested for HIV and were attending antenatal care services at facilities in the Hhohho region for the first time during the study period. We calculated the sample size following the approach proposed by Kohn et al and Hulley at al [51,52]. We based the calculation on results from the sentinel surveillance report which showed that 41% were HIV positive and 59% were HIV negative [45], to detect the difference in parental monitoring proportion of 28% and 52% [53] among HIV positive and negative participants respectively at α = 0.05, β = 0.2. Based on these, a total sample size of 149 (for both groups) was sufficient to detect this difference. Taking into consideration the complex sample design effect of 2.0 [54], we inflated the sample size by a factor of two, resulting in a sample size of 298.
We further increased the sample size to 596 by multiplying by a factor of two to ensure the statistical power is enough for multivariate analysis. Finally, the sample size was adjusted to 894, assuming a response rate of two-thirds due to the sensitive nature of our questionnaire.

Survey instrument
A self-administered structured questionnaire was developed in English based on the review of Swazi and international literature [47]. To improve the initial draft [47], we conducted a preliminary qualitative study during February-March 2015 using semi-structured in-depth interviews among 37 pregnant women recruited through purposive sampling. We recruited pregnant women in their 3 rd trimester to ensure that they would not be re-sampled for the current subsequent quantitative study. This initial step served several aims [55]. First, it allowed us to explore in-depth the sexual histories and ease of recalling those histories. Second, it enabled us to resolve language discrepancies to improve the translated draft. Lastly, it provided insights into recruitment issues. As described in our previous work, "the modified draft was then converted into an electronic format compatible with internet-enabled tablets, designed to be user-friendly and intuitive even for those participants not familiar with electronic devices" [47]. Using the tablet-based questionnaire, we piloted the instrument among 14 pregnant women (from a health facility not included in our survey sites) to test for face validity, skip logic, user interface, time to complete the survey and the upload-download functionality of the software.
The final survey instrument (S1 Questionnaire) consisted of a question on HIV status and seven domains: sociodemographic characteristics (6 items), schooling characteristics (2 items), HIV/AIDS-related knowledge (8 items), childhood household ownership of durable assets (19 items), obstetric characteristic (1 item), family characteristics (33 items) and sexual history characteristics (13 items). The family characteristics domain contained three items about parental characteristics and three subscales about family function: a) family cohesion subscale (8 items, Cronbach's alpha = 0.63), b) parental monitoring subscale (6 items, Cronbach's alpha = 0.67), and c) parent-child communication subscale (16 items, Cronbach's alpha = 0.83). The domain of sexual history characteristics explored the current and past sexual behavior. Items on HIV/ AIDS-related knowledge and sexual histories were in part taken from the Swaziland Demographic Health Survey [40]. In the absence of a locally validated family function scale, we adapted items from Family Adaptability and Cohesion Evaluation Scales IV (FACES IV) [56] as well as the Parent Monitoring Scale [53]. The instrument was translated into the local language (siSwati) by the bilingual researcher BWL and back-translated by another independent researcher to minimize translation dissonance.

Study design and sampling
The survey was a cross-sectional study using a simple two-stage cluster sampling strategy following Levy and Lemeshow [57]. The National Monitoring and Evaluation Office at the Ministry of Health in Swaziland facilitated us with the list of all 52 health facilities providing antenatal care services in the Hhohho region. Each facility was considered as a cluster in our study. In the first stage, we selected 10 clusters using simple random sampling without replacement. In the second stage, we enumerated 41 working days during August and September 2015, excluding Swazi Holidays and weekends, to serve as listing units. Then, we selected one working day to serve as a start date for the survey using simple random sampling. We estimated that twenty working days were sufficient to cover our desired sample size and prevent bias due to variations in weekly cycles. All pregnant women presenting at the 10 health facilities (10 clusters) from the random start date (17 August 2015) were consecutively screened for eligibility and invited to participate in the study. Recruitment took place throughout working hours in all facilities.

Data collection
To ensure high-quality data collection, we recruited nurses as field staff and provided them with a two-day intensive training; one day at a central location and another day at the data collection site. The field staff was trained on ethical considerations, aims and objectives of the study, the tablet use, and how to integrate the survey within patient flow. We followed a similar protocol for our previous research [47], having our field staff carry the print outs of screenshots of the electronic questionnaire to be able to read out loud and guide participants who had proficiency challenges without the field staff having to see their responses. BWL supervised data collection.

Statistical analysis
Sample weights and design effect. All statistical analyses were carried out using Complex Sample module of SPSS version 21 to account for the two-stage cluster sampling. We considered our sample self-weighted because, even though the selection of antenatal care clusters was done through simple random sampling at the first stage, at the second stage, pregnant women were consecutively sampled from all walk-in eligible potential participants, ensuring the sample size was potentially proportional to the total number of pregnant women attending each facility [54]. We calculated point estimates (proportions), their standard errors (SEs), and 95% confidence intervals (CIs) accounting for cluster sample design [57,58]. The magnitude of the inflation in variance was measured as the design effect, defined as "the ratio of the actual variance of a sample to the variance of a simple random sample of the same number of elements" [59].
Childhood household wealth index. Childhood household wealth index was developed according to the procedure described by Vyas and Kumaranayake [60]. Briefly, participants were asked if their childhood household had any of the 19 durable assets listed in the questionnaire (refer to S1 Questionnaire). Having the asset was coded as "1" and not having the asset as "0". The data was then analyzed using principal component analysis (PCA) which revealed that the first component included 10 items and accounted for 25.5% of all variance. Childhood household wealth index was defined as the total score of these 10 items weighted with the factor load of each item. After that, we ranked the participants into quintiles from poorest to the wealthiest according to their total score [61].
Family function. Family function consisted of three subscales (refer to S1 Questionnaire), to measure family cohesion, parental monitoring and parent-child communication, as previously stated. All responses of these subscales were 5-point Likert scale from "strongly disagree" to "strongly agree". In the analysis, responses were coded in the same direction such that higher scores represented "better family function" on all responses. For each subscale, we calculated the composite score, which was further divided into quintiles ranging from the lowest to the highest.
Bivariate and multiple logistic regressions. Bivariate analysis was performed using Chisquare tests for categorical variables to determine associations between HIV status and other variables. Factors that were significantly associated with being HIV positive at P value 0.10 were considered candidates to be included in the multiple logistic regression analysis. To provide a better fit for our multiple logistic regression model, we polychotomized continuous variables since their distributions were nonlinear. Out of 21 factors associated with HIV status at P value 0.10 in the bivariate analysis, 7 were excluded based on epidemiological importance or because they were subset questions of upstream questions like "currently in a polygamous marriage" a subset question for those who reported being married. There was no evidence of multicollinearity and singularity among the remaining factors. All 14 factors were compulsorily entered into the multivariate model to calculate the adjusted odds ratios (AORs) to assess the magnitude of independent association of these predictors with a self-reported HIV positive status.

Results
Of 894 eligible pregnant women invited to participate, 827 participants completed the study (response rate of 92.5%). The median age was 25 years; the youngest respondent was 18 years old and the oldest 43 years old. Table 1 displays the characteristics of respondents. About half of the respondents had completed at least secondary school (51.3%) and had ever dropped out of school (54.7%). Only 14.6% had ever stayed at a boarding school. The majority (84.9%) lived below Swaziland's monthly minimum wage (approximately $110 USD), did not have formal employment (58.2%), and were never married (58.5%). Most participants identified correct responses to HIV/AIDS-related knowledge questions, correct responses ranged from 83.1% to 96.0% (S1 Table).

Prevalence of self-reported HIV status by characteristics of participants
Overall, self-reported HIV prevalence was 35.9%. As displayed in Table 1, those who reported an HIV positive status were more likely to be older (P<0.001), have lower than high school educational attainment (P = 0.001), have ever dropped out of school (P<0.001), be selfemployed (P = 0.052), lived below Swaziland's monthly minimum wage (P = 0.039), never stayed at a boarding school (P = 0.005), have had a lower childhood household wealth index (P<0.001), in a polygamous union (P = 0.041), had two or more lifetime number of sexual partners (P<0.001), had multiple sexual partners (MSP) in the past 12 months (P = 0.001), used condom at last (P = 0.001) and first sex(P = 0.004), had sexual debut at 17 years or younger, experienced intergenerational sex at sexual debut (P = 0.025), had ever cohabited (P<0.001), did not know their first or current partner's HIV status (P = 0.005 or 0.042), and had ever experienced forced sex (P = 0.006). Marital status, religious services attendance, planned pregnancy, parental cohesion, parental monitoring parent-child communication, father with polygamous union or partners having MSP, ever had sex under the influence of alcohol and high HIV/AIDS-related knowledge were not significantly associated with a reported positive HIV status (P > 0.05)

Bivariate associations between independent variables and self-reported HIV status
As shown in Table 2

Multivariate analysis
As shown in Table 2

Discussion
In this study, we explored the association of structural and behavioral factors with selfreported HIV status among pregnant women in Swaziland, a country having the highest generalized HIV epidemic in the world. The high access rate to antenatal care services in Swaziland (98.5%) and high acceptance of HIV testing during antenatal care visits (95.3%) enabled us to assess HIV status without burdening participants with an additional HIV test [49]. Our study revealed that 36% of pregnant women were self-reportedly HIV positive with a peak rate of 52.3% in the age group of 30-34 years. Our findings are corroborated by recent national household survey data which showed that 39% women were HIV positive with a peak of 54% among the age group of 30-34 years, suggesting that our sample is unlikely biased in this respect [6]. We found that family function and HIV/AIDS-related knowledge had no significant statistical association with self-reported HIV infection whereas lower educational attainment, lower income, and certain sexual behaviors were significantly associated with selfreported HIV infection.

Familial factors
One of our study's most important findings was that family function (family cohesion, parental monitoring and parent-child communication) was not significantly associated with selfreported HIV status, even after controlling for other factors such as economic status. Though evidence from most published literature shows a significant association between constructs of family function with sexual reproductive outcomes such as sexually transmitted infections [11,12,[62][63][64][65][66], we did not find such an association in our study. There may be several reasons for this. First, family cohesion, parental monitoring, and parent-child communication may not have major influence on HIV infection risk in Swazi's context where the living arrangement and family structure are mainly of the extended family type [67] with generally higher family function compared to western societies. Western societies predominantly consist of nuclear family types and individualistic life styles [68], and many of the current studies were conducted in these contexts. The second reason may be that our participants were too homogenous in terms of family characteristics to detect such an association. In this case, future studies assessing family characteristics using cluster sampling should consider maximizing heterogeneity among participants by reducing samples within clusters and increasing the number of clusters as suggested by Kish [59]. Third, pregnant women may recall their personal childhood family circumstances and relationship differently, mediated by emotional and psychological changes induced by the current pregnancy. Forth, it is possible that existing family function scales are not sensitive enough to detect Swazi or African specific family function. If this is the case, there is a need for the development of more culturally specific assessment scales to assess family function in future research.
The only familial factor associated with HIV infection was the HIV-related death of a family member. Since participants having a family member who was infected with HIV appeared less likely to have multiple lifetime sexual partners (r = -0.135, P<0.001), it is possible that such association is not due to residual effect of statistically unadjusted sexual behavior but may be due to a more frequent HIV testing among participants with such family history.

Education and financial status
There was a clear inverse dose-response relationship between educational attainment and HIV infection; the higher the education attainment, the lower the reported HIV positive rate (40-50% rate among those with only up to primary or secondary education and 16.8% among those who had tertiary education). The association between education and HIV infection remained significant in the multivariate analysis. Educational attainment has long been recognized as a protective factor by the World Bank and since 2004 by the Global Coalition On Women and AIDS (UNAIDS Initiative) which have advocated for the exemption of school fees and the encouragement of HIV prevention education in schools [69,70]. As a result, every Swazi child is entitled to free primary school education in public schools. This policy has obvious positive outcomes as 95.3% of girls of schooling age are now able to read and write [71]. However, our results suggested that keeping girls in school only until primary education may still be insufficient to reduce the risk of HIV infection and further suggesting the amendment of national policy to safeguard girls' school enrollment until high school. Moreover, though enrolled in the education system, as much as 55% of participants reported to have dropped out due to lack of financial support (30%, S2 Table). As reviewed by Hardee and colleagues, girls face numerous barriers to stay in school such as lack of money to buy uniforms and textbooks. In addition, inadequate sanitary facilities also discourage girls to attend school especially during menstruation [19]. Such poor attendance may lead to low academic performance resulting in dropouts later on. Efforts should ensure not only to encourage higher educational attainment but also the uninterrupted school attendance among Swazi population, particular the girls, as such interventions have shown effectiveness in HIV risk reduction in the neighboring South Africa [28].
Regarding economic factors, ecological indices such as the Gross National Income has been shown to be inversely related to national HIV prevalence in SSA [72]. Similarly, at the individual level, a higher HIV prevalence is well documented in women with lower economic status [21,73,74]. In addition, it is evident that economic empowerment and cash transfer interventions targeting women have resulted in lower risky sexual behaviors [19,20,75]. Furthermore, a recent analysis in South Africa showed that cash or cash-in-kind reduced HIV risk among girls by mitigating pathways of poverty that increased their vulnerability [76]. While there is plenty of anecdotal evidence suggesting a link between poverty and HIV in Swaziland, empirical evidence from studies with methodological rigor are limited [10,77] prior to our study. Though Miller and colleagues identified models of transactional sex in Swaziland indicating possible mechanisms through which low income might lead to HIV risk, the research is not an epidemiological study [78]. In our study, we found a clear dose-dependent relationship between lower economic status and HIV infection with both current cash income and childhood household wealth index. While the latter index lost statistical significance in the multivariate analysis probably because of the relatively strong association it had with level of education (r = 0.39), childhood household wealth index may contribute to HIV vulnerability through poor educational attainment. In other words, while current low income may directly put women in socially vulnerable situation to HIV infection, childhood household wealth status may also affect HIV infection through limited education opportunities. However, further research should seek to identify these mechanisms to design appropriate interventions relevant to the Swazi context. We hope that this evidence will allow for better prioritization of HIV prevention interventions that focus on economic empowerment of women.

Sexual behavior-related factors
Many of the sexual-related factors identified to increase the risk of HIV infection in this study have been well documented in previous studies in many countries including those in SSA. In our study, !2 lifetime number of sexual partners was the most prevalent (75%) and a powerful predictor of HIV infection (AOR > 3). It is important to note that half of the women who had MSP cited financial benefit as a reason; strongly suggesting that poverty perpetuates the practice of MSP in Swaziland. Ever cohabiting was also found to be a strong predictor of HIV infection (AOR > 2) and associated with the highest HIV prevalence (53%). In recent years, cohabiting is on the rise in Swaziland due to the inability of men to pay bridal payment (dowry) as a pre-requisite of marriage (a practice prominent in Swaziland) leading men to cohabit with multiple women for longer period of time, thus increasing unprotected coital frequency which results in an increased risk of HIV infection [9,79]. An alarming finding in the Swazi context, is the fact that 75% and 30% of women had first sex and last sex respectively without knowing their partner's HIV status and had an elevated risk for HIV infection (AOR = 1.6 and AOR = 1.5). As a country with a highly generalized HIV epidemic, as high as 30-40% on average in both men and women [6], revitalization of campaigns to promote safe sex with a partner of unknown HIV status, as well as support programs to encourage couple testing and HIV status disclosure should be prioritized.

HIV/AIDS-related knowledge
Finally, HIV/AIDS-related knowledge level was generally high: 80-90% of respondents correctly identified that a healthy looking person can be HIV positive, the risk of HIV infection can be reduced by avoiding MSP and using condoms. This suggests that young women in Swaziland are engaging in HIV risky behaviors not because of lack of knowledge. Due to the crosssectional nature of our study, it could be argued that respondents may have recently gained HIV/AIDS-related knowledge during recent antenatal care visits and thus, their past risky sexual behaviors were primarily due to lower knowledge levels prior to antenatal checkups. Nonetheless, our data does not support this view since only 9.4% of respondents reported "lack of knowledge of HIV risks" as a reason for MSP. Furthermore, high HIV/AIDS-related knowledge has been previously reported in national surveys; e.g. 80-90% of women in the Swaziland Demographic Health Survey (2007) correctly identified ways to reduce HIV infection [40]. This is also consistently true among all age ranges, counter-arguing the concern that young people may not have had adequate information before their sexual debut, hence, thrusting them into risky behaviors. Data from the Multiple Indicator Cluster Survey (2010) is in concordance, demonstrating that the general public is well-equipped with adequate knowledge [41]. For these reasons, risky behaviors are unlikely due to lack of knowledge but most likely because of low income and low educational attainment as discussed above. As demonstrated by our findings, the gap between knowledge and practice is yet of great concern. The Extended National Multisectoral HIV and AIDS Framework has pointed this out by stating that "HIV and AIDS awareness and knowledge has not translated into the desired levels of behavior change due to inadequate personal risk perception that focus on translating knowledge into action" [10] noted in 2012. As the gap is still largely predominant in our findings, therefore the country urgently needs more innovative strategies and revitalization of existing ones because interventions centered on HIV/AIDS-related knowledge alone may not be sufficient to deter women from engaging in HIV risky sexual behavior.

Strengths and limitations
This study was designed to maximize internal and external validity. First, the study was conducted in the region where HIV prevalence among pregnant women is highest. Second, simple two-stage cluster sampling was adopted to ensure the representativeness of pregnant women with a systematic effort to maximize response rate (92%). Third, appropriate statistical procedures were adopted to adjust for clustering effect on the variances of point estimates. Fourth, the study was conducted using self-administered questionnaire with internet-enabled tablet devices to minimize interviewer bias and socially desirable responses on the sensitive issues of HIV status, income and sexual behavior. In spite of these efforts, this study has some limitations. First, recall bias could have been introduced since our questionnaire asked retrospective factors such as first sex and childhood household belongings. Second, contamination of socially desirable answer is still possible to sensitive questions. Third, cause-effect relationship cannot be inferred due to its cross-sectional nature. Lastly, this study may not fully represent all women of reproductive age in Swaziland since women using contraceptives were not included therefore, the generalization of these findings should be done with caution.

Conclusion
Family function did not appear to increase the risk for self-reported HIV status among pregnant women attending antenatal care in our study. However, given the scarcity of studies exploring the role of family function in the specific context of the Swazi HIV epidemic, we recommend further studies. Taken altogether, our study showed that risky sexual behavior was unlikely due to the lack of HIV/AIDS-related knowledge but due to structural factors such as education and economic situation. Therefore, besides programs that promote HIV knowledge and safer sexual practice, interventions that address structural factors by ensuring opportunities for higher education and by providing sustainable financial support to young women should be promoted.
Supporting Information S1 Table. Descriptive and bivariate statistics for HIV related knowledge items. Descriptive and bivariate statistics for HIV/AIDS related knowledge items associated with self-reported HIV infection. Complex SE = Standard error of estimate under complex sampling analysis. DEFF = Design effect. P value calculated using the second-order Rao-Scott adjusted chi-square statistic (DOCX) S2 Table. Descriptive frequency statistics for reason of dropping out of school. This table shows distribution of reasons for dropping out of school and self-reported HIV infection. Ã Where excluded because they were considered too young to reliably know the reason for dropping out of school since they did not complete primary school education (DOCX) S1 Dataset. Dataset of this study, (SAV) S1 Questionnaire. siSwati and English version of the questionnaire. (DOCX)