Outcome Prediction of Consciousness Disorders in the Acute Stage Based on a Complementary Motor Behavioural Tool

Introduction Attaining an accurate diagnosis in the acute phase for severely brain-damaged patients presenting Disorders of Consciousness (DOC) is crucial for prognostic validity; such a diagnosis determines further medical management, in terms of therapeutic choices and end-of-life decisions. However, DOC evaluation based on validated scales, such as the Revised Coma Recovery Scale (CRS-R), can lead to an underestimation of consciousness and to frequent misdiagnoses particularly in cases of cognitive motor dissociation due to other aetiologies. The purpose of this study is to determine the clinical signs that lead to a more accurate consciousness assessment allowing more reliable outcome prediction. Methods From the Unit of Acute Neurorehabilitation (University Hospital, Lausanne, Switzerland) between 2011 and 2014, we enrolled 33 DOC patients with a DOC diagnosis according to the CRS-R that had been established within 28 days of brain damage. The first CRS-R assessment established the initial diagnosis of Unresponsive Wakefulness Syndrome (UWS) in 20 patients and a Minimally Consciousness State (MCS) in the remaining13 patients. We clinically evaluated the patients over time using the CRS-R scale and concurrently from the beginning with complementary clinical items of a new observational Motor Behaviour Tool (MBT). Primary endpoint was outcome at unit discharge distinguishing two main classes of patients (DOC patients having emerged from DOC and those remaining in DOC) and 6 subclasses detailing the outcome of UWS and MCS patients, respectively. Based on CRS-R and MBT scores assessed separately and jointly, statistical testing was performed in the acute phase using a non-parametric Mann-Whitney U test; longitudinal CRS-R data were modelled with a Generalized Linear Model. Results Fifty-five per cent of the UWS patients and 77% of the MCS patients had emerged from DOC. First, statistical prediction of the first CRS-R scores did not permit outcome differentiation between classes; longitudinal regression modelling of the CRS-R data identified distinct outcome evolution, but not earlier than 19 days. Second, the MBT yielded a significant outcome predictability in the acute phase (p<0.02, sensitivity>0.81). Third, a statistical comparison of the CRS-R subscales weighted by MBT became significantly predictive for DOC outcome (p<0.02). Discussion The association of MBT and CRS-R scoring improves significantly the evaluation of consciousness and the predictability of outcome in the acute phase. Subtle motor behaviour assessment provides accurate insight into the amount and the content of consciousness even in the case of cognitive motor dissociation.


Introduction
Attaining an accurate diagnosis in the acute phase for severely brain-damaged patients presenting Disorders of Consciousness (DOC) is crucial for prognostic validity; such a diagnosis determines further medical management, in terms of therapeutic choices and end-of-life decisions. However, DOC evaluation based on validated scales, such as the Revised Coma Recovery Scale (CRS-R), can lead to an underestimation of consciousness and to frequent misdiagnoses particularly in cases of cognitive motor dissociation due to other aetiologies. The purpose of this study is to determine the clinical signs that lead to a more accurate consciousness assessment allowing more reliable outcome prediction.

Methods
From the Unit of Acute Neurorehabilitation (University Hospital, Lausanne, Switzerland) between 2011 and 2014, we enrolled 33 DOC patients with a DOC diagnosis according to the CRS-R that had been established within 28 days of brain damage. The first CRS-R assessment established the initial diagnosis of Unresponsive Wakefulness Syndrome (UWS) in 20 patients and a Minimally Consciousness State (MCS) in the remaining13 patients. We clinically evaluated the patients over time using the CRS-R scale and concurrently from the beginning with complementary clinical items of a new observational Motor Behaviour Tool (MBT). Primary endpoint was outcome at unit discharge distinguishing two main classes of patients (DOC patients having emerged from DOC and those remaining in DOC) and 6 subclasses detailing the outcome of UWS and MCS patients, respectively. Based on CRS-R and MBT scores assessed separately and jointly, statistical testing was

Introduction
Attaining an accurate diagnosis remains one of the most challenging tasks with patients with Disorders of Consciousness (DOC) in the acute phase; an accurate diagnosis is crucial for prognosis validity because it influences medical management in terms of therapeutic choices and end-of-life decisions. Disorders of consciousness include a wide range of medical conditions in which patients present a global inability to interact with their environment in terms of wakefulness and awareness [1]. Among the broad nosology of DOC [2], three main ascending levels have been clinically identified according to behavioural criteria: Coma [3], Unresponsive Wakefulness Syndrome (UWS) [4], and a Minimally Conscious State (MCS) [5]. After an acute brain injury (BI), nearly 20% of patients suffer from DOC [6]; the majority of these patients fall into a coma, which is associated with a mortality rate of 40% within 6 hours in cases of traumatic BI (TBI) [7] and within 5 days in cases of non-traumatic BI (NTBI) [8]. Coma survivors then enter into a gradual process of consciousness recovery by evolving first into UWS and then into MCS until they emerge from DOC. However, the natural progression of recovery varies substantially across patients; between 10% [9,10] and 30% [11] of coma survivors remain in UWS after four weeks. Moreover, the speed of recovery and the type of injury influence the level of recovery. Approximately 50% of patients experiencing UWS at least one month after BI remain in this state one-year after their TBI [9,10,12,13]; this fraction increases to 85% post-NTBI [9,10,[12][13][14]. With regard to the same time conditions, 23% [15,16] and 43% of MCS [15] patients do not regain consciousness 12 months after TBI and NTBI, respectively. Therefore, the most pressing clinical issue in the acute phase is to determine whether the diagnosis of these patients as being in UWS or MCS is correct and whether it is transient and a precursor of recovery. Although neuroimaging based on magnetic resonance imaging (MRI) or positron emission tomography [17][18][19][20][21][22] and neurophysiological studies [23][24][25][26][27][28] have led to major changes in the nosology of patients with DOC, the current standard for evaluating such states remains clinical examination. The JFK Coma Recovery Scale-Revised (CRS-R) [29] is currently the best instrument for DOC evaluation [30] [31]; this instrument seeks signs of conscious awareness using several subscales based on motor and verbal behaviour. Despite these advances in the nosology of DOC, the prevalence of misdiagnoses is as high as 43% in the postacute and chronic phases [32] [33] and outcome predictability remains quite poor [30]. Further improvements in clinical assessment are accordingly necessary, especially when considered in the acute stage. Bias in diagnostic assessment in the acute phase may arise from erroneous quantification and interpretation of behavioural responses. Indeed, underlying motor, verbal or drive deficits [34] [35] may partially or completely inhibit intentional responses. Otherwise, the CRS-R scales require stringent criteria to define a certain level of consciousness, which in turn may lead to clinical underestimation of the content and amount of consciousness, if those criteria are not fulfilled. Therefore, exploring the broadest range of motor behavioural responses may result in a more accurate consciousness assessment that enables a more reliable outcome prediction. This longitudinal study presents the results of a combined CRS-R assessment with a new set of clinical tests measuring various motor signs in the acute phase.

Participants and study design
We enrolled 33 patients (20 males and 13 females) among 65 patients who were admitted to our Unit of Acute Neuro-Rehabilitation (Department of Clinical Neurosciences at the University Hospital of Lausanne, Switzerland) between October 2011 and June 2014 for acute neurorehabilitation.
The inclusion criteria included a diagnosis of UWS or MCS (according to CRS-R criteria [29], supported by the Multi-Society Task Force Report [4] [36] and the Aspen criteria [5]) within 28 days of brain damage; we assumed a short cut-off time to avoid patients with prolonged DOC who might have biased the outcome assessment due to their lower probability of recovering [29,37].
The exclusion criteria included a) current neuromuscular function blockers or sedation; b) a premorbid history of developmental, psychiatric or neurological illness resulting in documented functional disabilities at the time of damage; c) persistent acute illness or progressive systemic or neurological disease; d) fewer than three complete clinical assessments with the CRS-R throughout the study.
The first CRS-R evaluation was completed using our own set of clinical tests or observations referred to as the "Motor Behaviour Tool" (MBT), which will be described below.
We collected general patient information: Age, localisation of brain lesions from routine neuro-imaging, brain damage aetiology, total number of CRS-R performed, rehabilitation duration and delay between occurrence of brain lesion and the first CRS-R/MBT assessment, as between occurrence of brain lesion and the last CRS-R score.
All of the included patients received a standardised intensive programme of rehabilitation, including physical, occupational, neuropsychological and speech therapies totalling at least 3 hours per day.
The local Lausanne Ethics Committee approved this study (142-09) and the legal surrogates of all of the participants provided written informed consent.

Procedure
We designed a two-stage procedure.
First, the enrolled patients underwent at least three successive CRS-R assessments with the aim of ensuring a weekly evaluation. The first assessment established the initial diagnosis of UWS or MCS [29] [30], and the following CRS-R scores determined the DOC outcome; motor (score = 6 of CRS-R subscale 3) or communication (score = 2 of CRS-R subscale 6) recovery defined emergence from DOC [29]. We divided the patients into two primary classes: Patients recovering consciousness with functional object use and/or functional communication (class 1) and patients remaining in DOC (class 2). We made the same subdivision for UWS and MCS, which led to six additional subclasses: (a) UWS and (b) MCS patients evolving to non-DOC, UWS patients (c) remaining in UWS or (d) evolving to MCS, and MCS patients (e) worsening to UWS or (f) remaining in MCS. We performed nine outcome comparisons: One global comparison between both main classes 1 and 2, and eight additional subclasses' comparisons encompassing four comparisons within the UWS patients and within the MCS patients, respectively. As a result, for the UWS patients, we compared subclass (a) with subclass (c), subclass (a) with subclass (d), subclass (c) with subclass (d), and subclass (a) with subclasses (c) and (d) together, which yielded a comparison between UWS patients emerging from DOC and those remaining in DOC. Likewise for the MCS patients, we compared subclass (b) with subclass (e), subclass (b) with subclass (f), subclass (e) with subclass (f), and subclass (b) with subclasses (e) and (f) together, which yielded a comparison between MCS patients emerging from DOC and those remaining in DOC. We analysed the first five CRS-R subscales because their scores defined the criteria for UWS and MCS, while the total score and the arousal subscore do not.
Second, we completed the CRS-R evaluation using the MBT evaluation, which was performed twice: At the first clinical evaluation with CRS-R, and two days later for confirmation. The CRS-R requires stringent response criteria for each subscore to assign a behavioural level and particularly to move from a low level of motor reflex behaviour (subscores 1-4) or absent verbal response (subscore 5) to the next level, which integrates the first clinical signs of consciousness. The application of stringent criteria means that insufficient but true signs of consciousness may not be quoted as an indication of consciousness, but rather as a reflex behaviour. Moreover, concomitant deficits or pathologies, including neuromyopathy, aphasia, or severe disorders of drive and motivation, additionally hamper motor or verbal responses [34]. Hence, both conditions lower the CRS-R score, leading to misdiagnoses of DOC.
We designed the MBT to complement the description of limb, facial, ocular and oral motricity, and verbal behaviour. This tool consists of a set of 10 clinical items that explore different positive motor signs (items 1 to 5) that the CRS-R scheme may overlook, medical conditions (items 6 and 7) that may hide conscious signs (negative signs) and various reflex responses (items 8-10) ( Table 1). The kinematics of non-reflex or intentional movement were defined in opposition to the kinematics of spinal motor reflexes and the nociceptive withdrawal reflexes triggered at rest; for example, the maximal elicitation of the mechanical reflex response in the upper limb due to a nociceptive stimulation applied on the index finger, consists of wrist adduction (frontal plane), elbow flexion (sagittal plane), and shoulder anteflexion (sagittal plane) occurring in two planes [38]. The first item looks for any limb movement whose kinematics differ from the motor reflex response in terms of orientation planes and the type of elicited muscles; such movements may be spontaneous or induced by a sensitive stimulation. The second item explores any isolated ocular movements that may be related to a hint of fixation or pursuit. The third item looks for hints of isolated intentional movements elicited by verbal command; reproducibility is typically lacking due to concomitant disorders, such as perseveration or drive disorders. The fourth item looks for any hint of a grimace induced by a noxious stimulus. The fifth item explores any motor behaviour that can be enhanced by a motivational context, such hearing a familiar voices or engaging in outdoor therapy. The sixth and seventh items take into account concomitant pathologies, including cranial nerve palsy, myopathies, and severe disorders of drive, which may hide facial motion or vocalisation, respectively. The last three items looks for increased motor reflexes triggered by simple stimulation (limb and oral, items 8 and 10 respectively) and for roving eyes.
The MBT uses a non-cumulative binary scoring system with 1 and 0 indicating the presence or absence of a clinical item, respectively. Since the MBT complements CRS-R measurements, we expected the MBT to weight positively the score of the first five CRS-R subscales, when the latter are quoted at the reflex level for the motor responses (scores 1, 1, 2, and 1 for the auditory, visual, motor, and oromotor functions, respectively) and at 0 (none) for the communication function. When at least one positive MBT sign (items 1-5) is present, the principle is to increment progressively (step of 0.1) the value of the CRS-R subscale when quoted at the reflex level, until attaining the next score that highlights the first clinical signs of consciousness. For example, if the visual function scale is set to the reflex level of 1, then this value is progressively increased (1.1, 1.2, 1.3,. . ., 1.9) when the first positive MBT sign is observed. We hypothesise that MBT items 1, 3 and 5 increase all five CRS-R subscales; item 2 increases the auditory and visual function scales, and item 4 increases the oromotor and motor function scales (Table 2). We performed statistical analysis on each increment to identify the critical point discriminating the different outcome of classes and subclasses.
Two members of our research team, which includes a physician, a neuropsychologist, and a nurse, conducted the CRS-R and MBT assessment using video recordings.
Since patients share the same kind of structural brain lesions with different DOCs, or present different lesion localisations within the same consciousness disorder, structural MRI cannot differentiate states of consciousness [17]. We performed MRI morphometric analysis on our data to confirm this statement.

Statistical analysis
We performed three methodological steps in our statistical analysis.
The first step consisted of analysing the CRS-R data. First, we performed the statistical prediction of DOC outcome using the first CRS-R subscores. Our statistical testing was based on a non-parametric Mann-Whitney U test because of the multinomial distribution of the CRS-R data. Second, since the CRS-R assessments were made across patients at different time points and with variable frequencies, we fit the longitudinal CRS-R data of each patient with a Generalised Linear Model (GLM) to obtain valuable temporal information regarding the DOC evolution of all classes/subclasses. The model regressors for the GLM consisted of four polynomials with progressive degrees ranging from 0-3 (constant, linear, quadratic and cubic polynomials) defining a polynomial regression model in one variable, referred to as a cubic model. We orthogonalised the polynomials such that the measured CRS-R variable could be projected into a canonical space spanned by the regressors. We then used the polynomial regressors according to a combinatorial process. As a normality test, we adopted the Lilliefors test [39], which revealed that the calculated regression coefficients followed a Gaussian distribution. We applied a one-sample two-tailed t-test on the regression coefficients, and then we calculated the temporal mean curve with its standard error for each class and subclass.
In the second step, we statistically predicted the DOC outcome from the MBT values also using a non-parametric Mann-Whitney U test because of the Bernoulli distribution of the binary MBT data. We computed sensitivity, specificity, and Yule's Q coefficient to test the performance of the statistical comparison.
The third statistical step consisted of assessing the outcome prediction on each increment of the CRS-R subscores enhanced by the MBT to identify a possible critical point discriminating the DOC outcome. The multinomial distribution of the CRS-R scores justified the application of a non-parametric Mann-Whitney U test.
Our study focused on a primary endpoint-the recovery prediction from DOC at discharge from our unit-and a secondary endpoint-the combined endpoint referring to functional walking ability and the likelihood of returning home.
We performed all of the statistical comparisons for each step between classes 1 (patients recovering consciousness with functional object use and/or functional communication) and 2 (patients remaining in DOC) and between the following subclasses: UWS patients evolving to non-DOC compared with UWS patients remaining in UWS and/or evolving to MCS; MCS patients evolving to non-DOC compared with MCS patients worsening to UWS and/or remaining in MCS. We applied the Bonferroni-Holm multiple comparison procedure to control the family-wise error rate. We performed the statistical testing in Matlab 12.0 (Math-Works, Natick, Massachusetts, USA).
For multiple comparisons correction, we applied a false discovery rate (FDR) control method in VBM. We used Matlab 12.0 for the algorithm implementation of statistical testing and SPM.

Results
In Table 3, we present detailed information about individual patient demographics (i.e., age, localization of brain lesions, BI aetiology, total number of CRS-R performed until discharge from the unit, rehabilitation duration and the delay between the occurrence of brain lesions and the first CRS-R/MBT assessment, as between the occurrence of brain lesions and the last CRS-R score).
For 31 of the enrolled patients, the CRS-R evaluation could be performed weekly (mean interval between evaluations: 7.5 ± 2.9 days), which yielded a significant correlation between the number of CRS-R evaluations and the duration of patient care (correlation coefficient of 0.59 with p = 0.0005). Patients 5 and 6 could not be followed weekly, but they were still selected for the study since they satisfied the eligibility criteria. Our general results comparing classes 1 and 2 for each aforementioned characteristic are listed in Table 4. General patient information was matched for both classes 1 and 2 (p>0.05), particularly in terms of brain lesion localisation, brain damage aetiology, the number of CRS-R evaluations, the delay between the occurrence of brain lesions and first CRS-R/MBT assessment, and the delay between the occurrence of brain lesions and the last CRS-R assessment.
Among the nine comparison analyses performed within each statistical step, four outcome comparisons exhibited significant differences and are discussed in detail below. Therefore, the results of the following subclasses' comparisons are not discussed: For the UWS patients, both comparisons between subclass (d) and subclasses (a) or (d) because of their insignificant differences, and the three comparisons involving subclass (e) since no MCS patients worsened to UWS.
First, a statistical comparison of the first CRS-R subscales did not permit us to differentiate between the patients recovering consciousness (class 1) from those patients remaining with DOC (class 2) ( Table 5). In the GLM analysis of the longitudinal CRS-R subscales, only combined together no-order (baseline) and first-order (slope) polynomial parameters, which outline the linear time course of the data, exhibited significant differences (p<0.02) when compared between both classes 1 and 2, between UWS subclass (a) and UWS subclasses (c) and (d) together, and between both MCS subclasses (b) and (f). Additionally, those time course differences yielded the following information: Temporal discrimination was not possible within the first 19 days for all subscales and the cut-off scores for the diagnosis of consciousness recovery were attained after 29 days for the motor function subscale and after 40 days for the communication subscale (Fig 1). Second, a statistical comparison on the MBT values revealed that all positive (items 1-5) and negative (items 6 and 7) MBT signs yielded a significant predictive value for discriminating between classes 1 and 2; an exception was observed for item 7 in the comparison of both classes 1 and 2 (p>0.05). We also observed the same results for UWS patients when we compared subclass (a) with subclass (c), and with subclasses (c) and (d) together. Moreover, the three reflexes items (MBT 8-10) also presented predictive properties for overall DOC patients (a comparison of classes 1 and 2) and UWS patients (subclass (a) compared with subclasses (c) and (d) together). On the other hand, comparisons of other subclasses (comparison between the (b) and (f) MCS subclasses and between (a) and (d), and the (c) and (d) UWS subclasses) did not reveal statistically significant differences. The statistical comparison was characterised by elevated sensitivity, specificity, and Yule's Q coefficient. Some sensitivity values were equal to 1, which was incompatible with the expected Bayes error rate (minimum error bound); this finding may be explained by the small sample size of some subclasses. The results are detailed in Table 6. Third, a statistical comparison of CRS-R subscales weighted by the positive MBT signs allowed those subscales to become predictive for overall DOC outcome (class 1 compared with class 2) and for UWS patients ((a) subclass compared with (c) subclass, and with the (c) and (d) subclasses). Even if they had a low increment (0.5), all of the items of the positive MBT signs were particularly valuable for enriching all of the CRS-R subscales (p<0.019). On the other hand, differences between MCS subclasses (b) and (f), between UWS subclasses (a) and (d), and between UWS subclasses (c) and (d), were not statistically significant ( Table 5).
The absence of significant differences between the MCS subclass compared with either the UWS subclass or with the non-DOC subclass can be explained by the small sample size and additionally by the closeness of the CRS-R subscores between these states.
Finally, VBM of brain lesions did not reveal any significant anatomical differences between classes 1 and 2 (p>0.05).

Discussion
In this study, we systematically evaluated acute DOC patients using the CRS-R scheme and of our MBT; the latter has been designed to identify clinical signs of consciousness that the CRS-R scheme may overlook, thereby leading to DOC misdiagnoses by lowering the CRS-R score. Table 5. Statistical prediction of the outcome from the CRS-R scores alone and weighted by the MBT scores (p-values corrected). Statistical prediction of the outcome from the first CRS-R subscores (auditory, visual, motor and oro-motor functions, and communication subscales) and from the same CRS-R subscores weighted by the MBT clinical values, assessed in the acute phase (mean 11.2 days after brain damage  We present three primary findings: 1. A statistical comparison applied to the first CRS-R evaluation in the acute stage did not yield predictable properties, meaning that an accurate diagnosis and prognosis cannot be reliably attained during this critical period using only CRS-R scores. However, regression modelling on the CRS-R subscales enabled us to differentiate over time the patients remaining with DOC from those having emerged from DOC, but the outcome discrimination was not significant in the first 19-22 days, regardless of the CRS-R subscores. Moreover, we found that at least 29 days is required to attain the cut-off scores for the diagnosis of consciousness recovery given by the motor function and the communication subscales. Our data corroborate the understanding that the prognostic validity of the CRS-R remains unproven in the acute phase [30] [33] despite its good content validity [30] and its recommendation as a tool for establishing diagnosis and monitoring recovery of consciousness [31].
2. Our statistical comparison revealed that the positive items of the MBT help to better identify signs of consciousness and provide reliable predictability addressing consciousness recovery. All of those items check for slight motor activities, which are by definition overlooked by the CRS-R, which imposes strong criteria to consider a motor behaviour as the expression of consciousness. Concretely, the CRS-R does not support non-systematised hypokinetic movement, hieratic oculomotricity, the draft of intentional motor responses, and doi:10.1371/journal.pone.0156882.t006 Outcome Prediction of Disorders of Consciousness in the Acute Stage slight induced facial motricity by noxious stimulus or increased motor behaviour due to a motivational context. This leads to negative scoring, whereas such motor features may express a conscious activity, especially since concomitant motor or cognitive impairment hamper motricity [34]. The two negative MBT items also share predictive properties, in the sense of enlightening concomitant medical conditions, which offer a credible alternative to the explanation of impaired verbal or motor response. Finally, the presence of reflex behaviours speaks for increased consciousness impairment and poorer outcomes.
3. With regard to the reliable predictability of the positive MBT items, it became apparent that their introduction into the CRS-R assessment rendered the CRS-R subscores also significantly predictable, meaning that strong quoting rules do disservice to clinical evaluations of consciousness.
These results lead to two primary potentialities that should be discussed. First, motor behaviour evaluation in the acute phase is a main way to accurately assess the content and the degree of consciousness and to predict outcome within the first 6 weeks. The fact that subtle motor behaviour can promote the predictability of DOC outcome, whereas the CRS-R subscores cannot, highlights the importance of exploring and considering all levels of motor activity that can reveal preserved consciousness. As a result, the CRS-R remains a valuable scheme that should be adapted to refine grading stages. With this finding in mind, additional studies should emphasise the performance analysis of all sub-items included in all the available evaluation schemes.
Second, our findings provide significant evidence that a reliable measure of conscious awareness in the acute phase necessarily needs to take into account the patient's capacity to perceive, understand and interact with his environment, his awareness of himself and his reaction to pain. Developed from evaluations starting as early as 24 hours after sedation withdrawal, MBT emphasises the observation of the broadest levels of subtle motor signs that may be seen as non-reflexive intentional responses in opposition to reflexive motor reaction; additionally, MBT takes into consideration concomitant medical conditions, such motor or communication disabilities, that hamper the patient's interaction with his environment [42]. The significant outcome prediction for both positive and negative MBT items, in terms of consciousness recovery and improvements in walking skills, may highlight the involvement of blocked motor efference rather than a true consciousness disorder. Patients presenting the "trap" of blocked motor efference might demonstrate the clinical signs of a new nosological entity: Cognitive motor dissociation [43], a condition in which "a lack of purposeful motor behaviour" hides motor response due to an underlying structural disruption between the motor cortex and the thalamus [44]. This condition leads to consciousness impairment overestimation, while the degree and level of consciousness are largely preserved. Establishing that a patient has such a neurological condition is of great significance with regard to therapeutic choices and global medical management because of a much better prognosis.
Additional improvements to MBT might investigate case-specific questions and address each patient individually by varying the testing conditions. Moreover, advances in neuroimaging technologies and the integration of electrophysiological paradigms into a reliable communication tool [45] might provide additional indications of awareness signs that ultimately lead to reducing the incidence of misdiagnoses and ensuring better treatment.

Conclusion
The identification and classification of enlarged motor behaviour, without restrictions on their characteristics and the localisation of lesions along with their expected related symptoms, should be systematically considered to overcome the aforementioned limits of the existing scales. Such work would improve diagnostic and prognostic knowledge in DOC care in the acute phase.