Distribution and Localised Effects of the Invasive Ascidian Didemnum perlucidum (Monniot 1983) in an Urban Estuary

Didemnid ascidians are notorious marine invaders, fouling infrastructure in many ecosystems globally. However, there have been few reports of direct interactions with native species in their natural environment. The invasive colonial ascidian Didemnum perlucidum was discovered in the Swan River estuary (Western Australia) growing on the native seagrass Halophila ovalis. Given the known effects of other related Didemnum species it was expected that D. perlucidum could adversely affect the seagrass, with possible flow on effects to the rest of the ecosystem. This study aimed to document the distribution and abundance of D. perlucidum in the estuary, and to determine whether this species had a negative impact on H. ovalis or associated flora and fauna. D. perlucidum was largely present near areas of infrastructure, particularly mooring buoys, suggesting these were the source of D. perlucidum recruits on the seagrasses. It showed a clear seasonal pattern in abundance, with highly variable cover and colony size. D. perlucidum had a measurable effect on H. ovalis, with colonies enveloping all plant tissue, likely restricting the photosynthetic ability of individual leaves and total plant biomass. There were also significantly less seagrass-associated mud snails (Batillaria australis) where D. perlucidum cover was high. These results demonstrate the ability of invasive ascidians to colonise and affect native seagrasses and associated biota. Seagrasses are pivotal to the ecological function of many urban estuaries world-wide. Biodiversity in these systems is already vulnerable to multiple stressors from human activities but the potential stress of fouling ascidians may pose an additional and increasing threat in the future.


Introduction
The introduction of non-indigenous species (NIS) that become invasive is an environmental challenge affecting the world's oceans and coastal ecosystems, including estuaries [1,2,3]. The effect is accelerating rapidly as vectors for introduction continue to increase [4,5].
Estuaries are semi-enclosed coastal water bodies with a free connection to the open ocean and within which seawater is measurably diluted with fresh water from land drainage [6].
Department of Water estimating coverage of about 403 hectares in 2011 [10]. However, as is the global pattern, H. ovalis has been declining in the Swan River, primarily due to human activities and stressors including increased temperature and sedimentation, excessive nutrient runoff, seaweed proliferation and invasion of NIS [3,29,30]. The introduction of the invasive colonial ascidian D. perlucidum may be an additional threat to the health and abundance of the native seagrass H. ovalis which could have 'flow-on' effects to the rest of the ecosystem. Consequently, it is important to identify whether there is an effect of D. perlucidum on H. ovalis, the scale of the effects and whether there are likely irreversible large-scale future impacts. The aims of this study were to 1) quantify the distribution and abundance of D. perlucidum on natural and artificial substrates in the Swan River under different seasonal conditions, and to 2) determine whether the colonies of D. perlucidum could be causing negative effects on Halophila ovalis and associated flora and fauna, particularly Batillaria australis.

Distribution and temporal variation in abundance of Didemnum perlucidum
To establish the distribution and temporal variation in abundance of D. perlucidum growing on seagrasses and infrastructure, 12 sites were surveyed across the lower reaches of the Swan River between the river mouth and the Narrows Bridge, about 15 km upstream (Fig 1). Permission for surveying the sites was granted by a permit from the Swan River Trust and all flora and fauna sample collections were supported by permits from the WA Department of Parks and Wildlife. Snorkelers and SCUBA divers surveyed seagrass meadows, moorings, and jetty pylons in April, June and August 2014 to document the seasonal peak through to the decline in D. perlucidum abundance as well as a follow-up in March 2015 to confirm the reappearance the following year. Within each site, 3 x 60m parallel transects were surveyed perpendicular to the shore. Six random 0.25x0.25m quadrats were observed and photographed at 20, 40 and 60m along each transect. Within the quadrats, percent cover of seagrasses and D. perlucidum was estimated. Observations of cover for seagrasses and D. perlucidum were classed into categories of 0, 1-10%, 11-25%, 26-50%, 51-75%, 76-90% and 91-100%. The midpoint of each of these cover categories was used as the reported value, to allow for calculating average percent cover and standard deviation [10]. Observations and photographs were recorded at adjacent moorings and jetty pylons to determine presence/absence of D. perlucidum on artificial structures adjacent to seagrass meadows.
Statistical analyses were performed using Primer 6 software [31] with the PERMANOVA add-on package [32]. Differences in cover of D. perlucidum among sites (random factor), times of the year (random factor) and transects (random factor nested in site) were tested by analysis of variance by permutation (PERMANOVA). Data was Log(x+1) transformed with Euclidean Distance resemblance.
For each site a range of factors were recorded to determine if any environmental conditions correlated with the distribution of Didemnum perlucidum. Measurements of temperature and salinity were taken at each site using a YSI multimeter probe. Baardseth's index was used as a proxy for water movement due to wave exposure at each site. This was calculated by counting the number of 10°sectors with a 3.75 km open fetch [33,34]. Thus, the exposure scale ranged from 0 = fully protected to 36 = fully exposed. Measurements were made on a marine chart with a 1:25,000 scale. This chart was also used to measure the distance of each site to the nearest jetty and to the river mouth. The number of moorings present within 100m by 100m around the measured transects was also recorded. Distance-based redundancy analysis (DISTLM) was used to identify the subset of environmental factors that best explained the distribution of D.
perlucidum and identify the correlation of each factor. For this analysis environmental data were normalised and D. perlucidum cover data was square root transformed with Bray Curtis resemblance plus a dummy variable to reduce the influence of many zero values.
A permanent 10m x 10m quadrat was established at Point Walter from April 2013 to March 2014 to further assess the growth and spread of D. perlucidum colonies over time. The entire quadrat was photographed, (100 1m 2 photos) every month. Image J software was used to measure the area of all emergent colonies. To test for differences in colony size within each quadrat from month to month we performed a repeated measures analysis of variance (ANOVA).

Effects on seagrass
Within five of the seagrass sites, (ZR, RB, MB, FB and PT, Fig 1), biomass samples were collected using randomly placed 10cm x10cm quadrats. Three samples of Halophila ovalis only and three samples of H. ovalis with Didemnum perlucidum were collected (total n = 30). The D. perlucidum was carefully separated from the H. ovalis and each sample was dried for 24 hours at 40°C and then weighed. A PERMANOVA (Euclidian Distance, 9999 permutations) was performed to test for significant differences in seagrass biomass between sites and samples with and without D. perlucidum. Photosynthetic response of Halophila ovalis leaves that had been covered with Didemnum perlucidum colonies were compared in the laboratory to leaves without D. perlucidum. Samples were collected from Freshwater Bay and Mosman Bay in February 2015 in approximately 2 metres depth. Five D. perlucidum colonies, along with five adjacent unaffected samples of H. ovalis, were collected from each site (total n = 20). Leaves from within the D. perlucidum colonies were then separated from the tunicate tissue. Pulse amplitude modulated (PAM) chlorophyll fluorescence parameters were measured from the middle of the leaf using a Mini-PAM (Walz, Germany). Maximum quantum yield (F v /F m ) was measured, as this is frequently used as an indicator of photo-physiological stress to the PS2 complex. Data were downloaded and analysed using the WinControl-3 software. All leaves were placed in plastic 'leaf clips' and dark adapted for 10 minutes. Rapid light curves were produced using an incremental sequence of actinic illumination with seven discrete irradiance steps (0, 45, 90, 180, 300, 500, 700 μmol quanta m -2 s -1 ). The maximum electron transfer rate (ETR max ) and photosynthetic efficiency (α) were calculated by fitting the rapid light curve data to an exponential function [35]. Pigment levels were measured by cutting a 1cm diameter disks from each leaf, measuring the wet weight and soaking them overnight in 3ml of 100% acetone. The following day, the remaining leaf tissue was removed and the acetone was mixed with 1 ml distilled water and 1ml methanol. Solutions were centrifuged for 3 minutes at 1500rpm and kept on ice. The top 3ml was measured using a spectrophotometer at 664nm wavelength to determine the concentration of chlorophyll a [36].
Photosynthetic activity and pigment concentrations can only be measured on leaves that are still alive. Consequently, this produced an overestimation of the health of the leaves within the D. perlucidum colony, as most of the leaves were dead. Therefore an index of the health of leaves within and without D. perlucidum colonies was estimated following the scale: 1 = alive (green), 2 = alive with necrotic patches (green with brown spots), 3 = dead (various shades of brown). This index was used to qualify the photosynthetic and pigment results.

Effects on Batillaria australis
Battilaria australis is a mud snail which is currently the most abundant macroinvertebrate in the Swan River [37]. These snails were sieved from sediment cores with and without Didemnum perlucidum colonies. Sediment cores (90mm diameter, 60mm depth) were collected from 3 sites (ZR, MB and FB) in April and June 2014 and March 2015. Within each site, 6 cores were collected, 3 containing a D. perlucidum colony and 3 without (total n = 18). B australis individuals were counted in each core and analysed by PERMANOVA (data Logx+1 transformed, Euclidean Distance resemblance) following a design of treatment (with or without D. perlucidum) (fixed), time of year (random) and site (random).

Distribution and monitoring of D. perlucidum
In April 2014, D. perlucidum was observed at 86% of the sites surveyed in the Swan River (S1 Table). Of those, 75% had D. perlucidum growing on seagrass, primarily on Halophila ovalis but in Rocky Bay, where other seagrasses are present, it was also growing on Zostera marina. Occasionally it was even seen growing on the macroalgae Gracilaria comosa, Cystoseira sp. and Chaetomorpha sp. Where D. perlucidum was associated with H. ovalis it was observed on individual seagrass blades but more often was spread across numerous blades, forming mats up to 30cm in diameter (~900 cm2). Most observations of D. perlucidum on seagrass were found within 10s of meters of colonies growing on artificial structures such as jetties and mooring lines. Colonies were recorded from both natural and artificial substrates in the Swan River between 2m and 10m depth (Fig 2). In June, D. perlucidum was only present on seagrass at 58% of the observed sites but was still widespread on artificial surfaces. In August, there was no observable D. perlucidum on either seagrass or artificial structures at any of the sites. In March 2015, D. perlucidum had returned on seagrass at 42% of the sites and artificial structures in 86% of the sites (Fig 1).
The cover of Didemnum perlucidum varied considerably over time (Fig 1 and Table 1). In April, mean (± SE) cover across the estuary was 3% (±1.4) with a maximum mean cover of 17% (±6.6) at Mosman Bay. In June, mean percent cover was 2% (±0.8) with a maximum of 7% (±4.4) at Mosman Bay. By August and through to the following year there was no D. perlucidum visibly present at any of the sites being monitored. By March 2015 it was present again, with a mean cover of 2.6% (±1.9). Again, the highest cover was observed in Mosman Bay, peaking at 22.6% (±3.9).
Within the permanent quadrats, at the beginning of the study in April, D. perlucidum colonies had an average size of 106cm 2 ± 7.3 SE with a range of 6.8 to 853cm 2 . Colony size contracted significantly by May and continued to decrease each month through the winter. By August there were no noticeable colonies. We continued to monitor the permanent quadrat until April 2014. In that time, D. perlucidum never returned.
Though D. perlucidum was not present within the permanent quadrat in April 2014, it was present about 100m away on Halophila ovalis clustered around a navigation marker. It was also present on the marker itself. Colonies within a 20m radius of the marker were measured to compare to the size of colonies from the previous year (n = 40). The average area of the colony  Table 1. Analysis of Variance by Permutaion (PERMANOVA) testing for differences in percent cover of D. perlucidum among sites (random factor), times of the year (random factor) and transects (random factor nested in site). The analyses were based on Euclidean Distances calculated from Log(x +1) transformed data. was 33.1cm 2 ± 6.6 and ranged from 1 to 196 cm 2 , demonstrating the extreme variability of colony sizes at that time.
Of the abiotic variables measured throughout the study, the multiple regression model (DistLM) found the number of moorings within a site to explain the highest proportion of variation in D. perlucidum abundance, although this was only about 16% of the variation in D. perlucidum cover across the range of sites and seasons. Salinity (14-39.4ppt, S1 Fig) contributed the second-most, explaining 10% of variation in cover (Table 2A and 2B). Water temperature ranged from 13.1°to 27°C (S1 Fig) and was expected to be highly correlated with abundance and distribution of D. perlucidum based on previous observations but in this model it was not significant. Maximum depth correlated significantly but only explained very little variation in distribution. Other factors including wave exposure and distance to jetties were not significantly related to the distribution and abundance (Table 2A).

Effects on seagrass
Halophila ovalis biomass was significantly lower when associated with D. perlucidum (p = 0.0003) (Fig 3, Table 3). In many cases, the plant tissue had disintegrated within the D. perlucidum colony. Differences in leaf biomass loss were not significant across the 3 sampling seasons (p = 0.059), implying that loss of biomass due to smothering by D. perlucidum did not change seasonally. The significant difference between the sites could be attributed to the spatial and seasonal variability in D. perlucidum and H. ovalis abundance (p = 0.001).
Differences in photosynthetic responses were not significantly different between live Halophila leaves with and without fouling by D. perlucidum (Fig 4). The differences between the rapid light were not statistically significant and at both sites, there were no significant differences recorded for ETRmax or α (ETRmax p = 0.247, α p = 0.251). Differences in chlorophyll a pigments were also not significant between leaves with and without coverage of D. perlucidum (p = 0.259).
Seagrass leaves within D. perlucidum colonies remained strongly attached to their stolons, which were rooted in the sediment. This suggests that leaves were alive when D. perlucidum settled and began to cover them rather than being dead or dying prior to settlement. This is further supported by the observation that D. perlucidum was seen growing on individual live leaves, and in sampling leaves around the D. perlucidum colonies it was evident that over 70% of the surrounding leaves appeared to be alive and healthy. The leaves chosen to measure photosynthesis and pigments were those which were still alive and photosynthetically active. As examined with the health index, this represented a small percentage of all the leaves within D. perlucidum colonies. Only 7% of the leaves within any given colony still maintained a bright green colour and only about 38% had some remaining green colour but were starting to turn brown, indicating that they were unhealthy or dying. The remaining 55% of leaves were various shades of brown suggesting they were already dead. This was compared to samples of unaffected leaves, in which more than 70% of leaves were green and alive (Fig 5). While photosynthetic rates and pigments of individual leaves might be marginally lower when associated with D. perlucidum, the measured leaves were part of a greater assemblage of mostly dead leaves. This suggests that measuring photosynthesis on the remaining live leaves may be underestimating the overall reduction in production capacity of H. ovalis. The lack of manipulation in the design of this study limits the ability to fully interpret these findings and used in isolation, fluorescence parameters are not a good indicator of stress in response to D. perlucidum.

Effects on Batillaria australis
There were significantly lower densities of B. australis in the presence of D. perlucidum associated with H. ovalis (p = 0.003). Average counts of B. australis were 1.3 to 2.8 times higher in cores without D. perlucidum than those with colonies present (Fig 6).

Discussion
Globally there has been rapid, large scale seagrass loss associated with multiple stressors including climate change, shifts in water quality and localised impacts such as pollution and increased sediment and nutrients [2,11,12]. The introduction of invasive species, particularly ascidians, is another stressor contributing to seagrass loss [25,26,27]. This study has shown that in the Swan River, a typical urban estuary, the invasive colonial ascidian Didemnum perlucidum has a widespread seasonal coverage throughout the lower reaches of the estuary with the potential to directly contribute to loss of the native seagrass Halophila ovalis. As far as we are aware, this is the first time that this species has been documented fouling on natural substrata such as live seagrass. We have shown that it has a measurable effect on small scale patches of seagrass, impacting the biomass of leaves that it settles on and also influencing the abundance of associated fauna. While it is difficult to measure, the photosynthetic ability of leaved fouled by D. perlucidum may also be reduced. The presence of human infrastructure, particularly boat moorings, was found to be the best predictor of D. perlucidum distribution in the estuarine environment, suggesting these structures facilitate the spread to, and impact on, seagrass beds.

Distribution of Didemnum perlucidum
In this study, the distribution of Didemnum perlucidum in the lower estuary of the Swan River was patchy and seasonal, and appeared to be driven by the presence of infrastructure and the hydrology of the river, particularly the changes in salinity. The abundance of D. perlucidum colonies was consistently most prevalent in areas with artificial structure including boat moorings, jetties, yacht clubs and navigation markers. The first observations of colonies each year were concentrated around these structures (Simpson personal observation), suggesting they facilitated recruitment into seagrass beds. This also helps to explain the mechanism of dispersal and recolonization of D. perlucidum each summer. Settlement arrays in the inner harbour area of Fremantle Port (at the river mouth) and around nearby Garden Island have demonstrated overwintering colonies (Simpson unpublished data). It is possible that these colonies provide larvae for recruitment upriver as conditions become more favourable. There may also be overwintering colonies in deep pockets of the river where salinity remains high even during winter flooding, such as in Mosman Bay which consistently had the highest cover of D. perlucidum. It is likely that D. perlucidum maintains small colonies  Didemnum perlucidum in an Urban Estuary on mooring buoys. Recreational vessels are a likely vector for spread of propagules from these surviving colonies throughout the river. In addition, rafting on plant material could be another transport vector explaining the reappearance and spread of D. perlucidum. Rafting is an important process for the population dynamics of many organisms including ascidians and it has a strong influence on coastal biodiversity. Rafting on eelgrass is a previously described method of transport and spread for other ascidian species including Botryllids and Ciona intestinalis and has been suggested as a method of spread for Didemnum vexillum [18,27,38,39]. H. ovalis is vulnerable to dislodgement as the leaves grow from a shallow rhizome with only small roots below ground as an anchor. As leaves become smothered by D. perlucidum the extra drag increases the likelihood that the rhizome will break or the plant will become uprooted. They roll along the substrate and when they get snagged may induce settlement in new areas. D. perlucidum is very capable of regenerating from fragmentation [20]. Colonies may overwinter in deep seagrass areas or on artificial substrate near the Port or on vessels but are able to spread quickly to other areas throughout the season through larval dispersal, fragmentation and/or rafting.
Hydrology of the river is also important in shaping the distribution of D. perlucidum. The Swan River is a microtidal estuary (tidal range <2m). Physical processes are driven by wind, wave action and freshwater runoff rather than tidal fluctuation. This results in a system of very low turbulence and high density stratification [40]. The hydrology of the Swan River changes seasonally due to the nature of winter rainfall and following catchment runoff [40,41]. Early in the summer, a salt wedge propagates upstream roughly 60km from the river mouth. As river flow increases in winter, there is usually a period of stratification before the salt wedge is pushed back out toward the lower estuary. Salt water remains longer in the deeper areas until the freshwater influx from rain eventually flushes the estuary [40]. This pattern of salinity helps to explain why D. perlucidum is able to survive so far upstream during the summer. Colonies were present throughout much of the lower estuary following periods of warm water and high salinity. As the salt wedge retreated with the influx of rainwater runoff, colonies retracted or died. D. perlucidum died off early near Point Walter because it is an area strongly influenced by freshwater runoff with low salinity by June. Mosman Bay and Chidley Point maintained large colonies later in the year because they are deeper sites which still had high salinity under the freshwater lens. By August the saltwater had moved out of the estuary, resulting in a complete lack of visible D. perlucidum survival throughout the winter. Salinity and temperature are important environmental factors influencing the distribution and recruitment of D. perlucidum [22,42]. Growth and reproductive effort are highest throughout the warmest months and larvae production, recruitment and growth decline during the winter [20]. Current knowledge of temperature thresholds suggest it can survive between 15 and 30°C [16] and in situ experiments have shown that D. perlucidum increases in cover and biomass when water temperature is increased [43,44]. D. perlucidum can reproduce throughout the year but there is a reduction in colony size and larvae density during the period of winter to spring [20,22]. But despite reduced recruitment, in a marine environment, D. perlucidum is able to continue to survive and reproduce throughout the year [22,42]. Within the Swan River, it is likely that the same pattern would persist. However, with the freshwater input through the estuary, the salinity becomes too low for ascidian survival and they appear to completely die off. In this study, our model suggested no evidence that temperature was a major driver in determining the distribution of D. perlucidum. This may be because although temperature is important to many aspects of the biology of D. perlucidum, salinity is most important in determining its survival. The salinity threshold has not yet been established but observations of D. perlucidum have shown a retraction of colonies in coastal environments during winter when freshwater runoff is increased, while colonies offshore do not seem to retract through winter (Simpson, personal observation). Multi-factorial studies need to be undertaken to determine the minimum salinity and temperature thresholds for survival and performance of this species.

Effect on seagrass
Compared to other plant groups worldwide, seagrasses require very high light levels to provide oxygen to their roots and support large amounts of non-photosynthetic tissue [2]. This light requirement means that seagrasses are highly influenced by environmental changes that alter light levels reaching the plant, such as turbidity [2], shading and fouling [26]. Fouling of D. perlucidum on Halophila leaves appears to cause the leaves to die and decompose within the colony. Ascidians can reduce PAR between 10 and 95% depending on the morphology of the zooids [26]. D. perlucidum would be particularly variable because the presence of calcareous spicules in the tunic increases attenuation. The PAM analysis suggests that D. perlucidum is having an effect on the photosynthetic ability of the plant tissue but it cannot be concluded that reduced photosynthesis is the mechanism killing the plant.
H. ovalis has a low tolerance to light deprivation, with complete plant death occurring after 38 days in the dark [45]. As D. perlucidum can persist for several months, the shading induced by this ascidian could clearly deprive the seagrass of the light required for effective photosynthesis. With limited tolerance to light deprivation, the long-term survival strategy of this species may be based on its ability to rapidly regrow from seed and/or vegetative fragments after light deprivation [45]. If the long term survival strategy of Halophila is limited by other anthropogenic stressors, the additive effect of ascidian fouling will put additional pressure on the decline of seagrass meadows.
In 2011, it was estimated that the Swan River estuary supported 403 ha of H. ovalis [10]. In its peak season, as shown by measurements from April 2014 and March 2015, D. perlucidum was present at 75% of the observed sites throughout the estuary, with its mean abundance being 3% cover. That would hypothetically equate to 9ha of H. ovalis being smothered by D. perlucidum during the peak season. Given an average biomass loss of 43%, regardless of season, that implies that as much as 3.9 ha of H. ovalis could be lost every year during the peak D. perlucidum season, the same area as 6 soccer fields. During the low season, as measured in June 2014, 58% of the estuary had a D. perlucidum presence, with a mean cover of 2% at affected sites. This equates to 4.7 ha with a total potential loss of 2 ha of biomass. While this is a hypothetical extrapolation, it provides an estimation of the potential loss of H. ovalis at the estuary scale.

Effects on Batillaria australis
Studies on community structure in marine systems have often shown that invading species have the potential to displace resident species [46,47,48] and in this study Batillaria australis was surveyed as an example of a benthic species that may be effected by the presence of an invasive fouling tunicate. This species was chosen as an example because it is the most abundant macroinvertebrate in the Swan River estuary [37]. It is also very important in creating habitat and facilitating overall diversity of benthic species [49]. There were significantly higher numbers of the mud snail B. australis in cores without Didemnum perlucidum colonies. Thomsen et al. [50] found the opposite response to be true when the macroalgae Gracilaria comosa covered Halophila. It created a 'habitat cascade' where habitat forming seagrass provided living space and protection for another habitat former (macroalgae) which then increased facilitation of invertebrate species including B. australis. However, where macroalgae provides oxygen, additional food and detritus used by the snails, D. perlucidum provides no nutritional benefit and at the same time seals off the decomposing seagrass within its acidic tunic. Didemnids have a pH within their tunic of less than 3, which is a level of acidity that deters most generalist fish predators [18,51] other snails [27] and may deter B. australis as well.
In 2012 it was estimated that there were over 5.2 billion snails in the seagrass meadows of the Swan River estuary, which has a massive impact on moving sediment, releasing nitrogen, filtering water and producing shells as potential substrate [10,37]. The peak abundance of B. australis also coincides with the peak abundance of D. perlucidum. Both species have the highest cover in the sites closest to the river mouth and both species appear to experience their highest recruitment during the late summer [10,22]. B. australis populations also support other organisms such as billions of macroalgae attached to living snails and >100 million hermit crabs living in empty shells [37] as well as providing habitat to communities of sessile invertebrates [49]. Further research is needed to determine the extent of interaction between these two species. However, given the abundance, distribution and ecosystem function that this species already has, the interaction of high numbers of D. perlucidum with 5.2 billion snails could eventually cause ecosystem effects that would be very difficult to measure or predict.

Conclusions
This study has demonstrated that Didemnum perlucidum is able to survive year after year colonizing native seagrass within an urban estuary. Its distribution is seasonal, patchy and largely driven by the presence of artificial infrastructure and changes in salinity. D. perlucidum has shown the potential to impact Halophila ovalis at the level of the individual plant through decreasing photosynthetic ability and loss of biomass. It has also shown the potential to interact with another species on a small scale.
Impacts from stresses on coastal marine communities are often manifested at the individual species level, but can magnify in effect throughout the entire ecosystem [13,52]. Recent findings show that extremely consequential impacts at the ecosystem level (ie. trophic cascades, changing nutrient cycling, etc) may not be easily detected or may remain innocuous for some time [5] particularly in situations such as an estuary where population dynamics of an invader and the dynamics of the ecosystem vary over space and time. This demonstrates the need for regular long-term monitoring at an ecosystem level. The decline of seagrass is continuing in urban estuaries around the world due to many factors such as nutrient eutrophication, human impacts and increasing temperatures [2,10,12] and it is these stressful conditions that enable invasive ascidian populations to thrive [27]. The potential for impact from D. perlucidum or any other invasive ascidian species should not be ignored or underestimated.