The Deubiquitinating Enzyme UBPY Is Required for Lysosomal Biogenesis and Productive Autophagy in Drosophila

Autophagy is a catabolic process that delivers cytoplasmic components to the lysosomes. Protein modification by ubiquitination is involved in this pathway: it regulates the stability of autophagy regulators such as BECLIN-1 and it also functions as a tag targeting specific substrates to autophagosomes. In order to identify deubiquitinating enzymes (DUBs) involved in autophagy, we have performed a genetic screen in the Drosophila larval fat body. This screen identified Uch-L3, Usp45, Usp12 and Ubpy. In this paper, we show that Ubpy loss of function results in the accumulation of autophagosomes due to a blockade of the autophagy flux. Furthermore, analysis by electron and confocal microscopy of Ubpy-depleted fat body cells revealed altered lysosomal morphology, indicating that Ubpy inactivation affects lysosomal maintenance and/or biogenesis. Lastly, we have shown that shRNA mediated inactivation of UBPY in HeLa cells affects autophagy in a different way: in UBPY-depleted HeLa cells autophagy is deregulated.


Introduction
Macroautophagy (referred to as 'autophagy' hereafter) is the major lysosomal degradation pathway of cytoplasmic components.The overall molecular mechanisms of autophagy are relatively well understood and are conserved in all eukaryotic cells from yeast to humans [1,2].The autophagosome formation complex which includes the class III P(I)3-kinase VPS34 and BECLIN-1 initiates the formation of an isolation membrane [3,4].Elongation of this membrane then involves two conjugation systems.The first system results in the association of the cytosolic microtubule-associated light-chain 3-I (LC3, also known as Atg8) with phosphatidylethanolamine to generate a lipidated LC3-II form.The second system forms the ATG12-AT-G5-ATG16 macromolecular complex.Both conjugation systems contribute to the completion of the double-membraned autophagosomes which eventually fuse with lysosomes to generate the degradative single-membraned autolysosomes.Originally described as a non-specific degradation process limited to bulk cytosol in response to starvation, autophagy is now known to be also responsible for the degradation of specific substrates, including senescent organelles, bacteria, viruses and aggregated proteins (reviewed in refs.[5,6]).
Ubiquitination is a major post-translational modification which results in the covalent linkage of one or several ubiquitin moieties on substrate proteins.It plays major roles in many cellular processes.In autophagy, it is involved in the regulation of the stability of autophagy regulators such as BECLIN-1 and BCL-2 [7][8][9].In addition, ubiquitin functions as a tag targeting specific substrates (protein aggregates, mitochondria or intracellular bacteria) to autophagic degradation [10][11][12].
To identify new DUBs of the USP and UCH sub-families that negatively regulate autophagy in vivo, we have systematically silenced by RNAi the corresponding genes in the Drosophila larval fat body.This tissue is the primary nutrient storage organ of the larva and produces a robust activation of autophagy in response to nutrient starvation [26].Moreover, it consists of a monolayer of large, polyploid cells which are ideal for imaging-based techniques [27].This screen identified four DUBs that may play a role in autophagy: Uch-L3, Usp45, Usp12 and Ubpy.We further showed that Uch-L3 and Usp45 did not act in a cell autonomous manner, whereas Usp12 and Ubpy did.Focusing on Ubpy, we have shown that its loss of function results in the accumulation of autophagosomes due to a blockade of the autophagy flux.Furthermore, analysis by electron and confocal microscopy of Ubpy-depleted fat body cells revealed altered lysosomal morphology, indicating that Ubpy inactivation affects lysosomal maintenance and/ or biogenesis.Lastly, we have shown that shRNA mediated inactivation of UBPY in HeLa cells also affects autophagy which appears to be deregulated with an increased number of autophagosomes and increased autophagy flux.

A genetic screen for deubiquitinating enzymes involved in autophagy identifies Ubpy
In order to identify new DUBs regulating autophagy, transgenes containing specific inverted repeats (IR) allowing for the production of double stranded RNAs targeting 25 DUBs of the USP and UCH families were expressed in the larval fat body along with the GFP-LC3B autophagic marker using the Cg-Gal4 driver line [28].The GFP-LC3B reporter encodes a fusion protein between the Green Fluorescent Protein and the human LC3B protein which is diffused in the cytoplasm and in the nucleus under normal conditions whereas upon autophagy induction, it is recruited onto autophagosomes [29].In order to identify regulators of autophagy, this screen was carried out on fed mid third-instar larvae that have a low basal level of autophagy (Fig 1A and 1C).Three USPs (CG4165/Usp45, CG5798/Ubpy and CG7023/Usp12) and one UCH (CG3431/Uch-L3) were retained as candidates for autophagy negative regulators because their silencing induced accumulation of GFP-LC3B positive dots in the cytoplasm of at least 50% of the cells (Fig 1A , 1C-1G and S1 Table ).Fat body specific inactivation of three other USPs was lethal at early larval stages and was not further analyzed (S1 Table ).The ability of these DUBs to regulate autophagy in a cell-autonomous manner was then tested using the FLPout method [30] to induce RNAi-dependent gene silencing in clones expressing the GFP-Atg8a reporter (Atg8a is a Drosophila paralogue of human LC3B) [31].This clonal analysis revealed that cell-specific silencing of Uch-L3 and Usp45 did not result in accumulation of GFP-Atg8a vesicles (Fig 1B , 1I and 1J).As such, these two DUBs may be putative regulators of autophagy at the systemic level, but not at the cellular level and were not further characterized.In contrast, cell-specific inactivation of Ubpy and Usp12 resulted in accumulation of autophagosomes (Fig 1B , 1K and 1L) indicating that Ubpy and Usp12 are putative cell-autonomous regulators of autophagy.We have used a second independent RNAi line targeting Ubpy [32] which also resulted in accumulation of autophagosomes (S1 Fig) .We have thus focused our investigation on Ubpy because it was not known to play a role in basal autophagy, despite this protein being extensively characterized for its role in endocytosis [33][34][35][36][37][38][39] and to a lesser extent in mitophagy [22].

Ubpy inactivation blocks the autophagy flux
An increased number of autophagosomes can either be caused by the activation of the autophagic flux or by the accumulation of basal autophagosomes due to the inhibition of their degradation.To distinguish between these possibilities, we first made use of a transgenic Drosophila line expressing the GFP-mCherry-Atg8a fusion protein [40].This protein produces yellow (green merged with red) fluorescence in autophagosomes and only red fluorescence in autolysosomes due to quenching of the GFP fluorescence in these acidic structures.In fed control larvae expressing this reporter along with a control RNAi transgene targeting Luciferase, no autophagosomes nor autolysosomes were detected (S2 The autophagy flux can also be monitored by assessing the degradation of known autophagic substrates such as the p62 protein, which accumulates upon autophagy flux blockade [42,43].Using a specific antibody raised against Ref(2)P, the Drosophila p62 homolog [44], we observed the accumulation of the Ref(2)P/p62 protein in Ubpy silenced cells compared to wild-type neighboring cells (Fig 2E and 2G).Altogether, these results unambiguously demonstrate that Ubpy inactivation results in a blockade of the autophagic flux.
We then made use of two transgenic lines expressing either the Flag tagged wild-type UBPY protein (Flag-UBPY WT ) or its catalytically inactive, dominant negative counterpart (Flag-UBPY C>S ) [45].Expression of the Flag-UBPY C>S protein resulted in the accumulation of GFP-Atg8a positive autophagosomes whereas expression of the wild-type protein had no effect (Fig 3A -3C).Analysis of the GFP-mCherry-Atg8a distribution in starved Flag-UBPY WT expressing cells showed that both yellow and red dots were observed indicating that autophagosomes and autolysosomes were present (Fig 3D and 3G), consistent with an increase of the autophagic flux in response to starvation.By contrast, in Flag-UBPY C>S expressing cells, we observed mostly yellow dots and no red dots indicating the presence of autophagosomes and absence of autolysosomes (Fig 3E and 3G).Lastly, Lysotracker staining of starved third-instar larvae fat bodies showed that Flag-UBPY WT expressing cells (Fig 3G ) were not different from wild-type neighboring cells whereas a nearly complete loss of Lysotracker staining was observed in Flag-UBPY C>S expressing cells (Fig 3H).These results show that expression of the dominant negative form of UBPY results in a blockade of the autophagic flux, mimicking the phenotype induced by RNAi.In addition to validating the specificity of the Ubpy-targeting RNAi transgenes, these results further indicate that the deubiquitinating activity of UBPY is required to maintain the autophagic flux.

Ubpy inactivation induces lysosomal defects
To get further insight into the role of UBPY in autophagy, we turned to ultrastructural analysis by electron microscopy.In wild-type larvae rare degradative lysosomes with cytoplasmic components at various stages of degradation were present (Fig 4A and refs.[29,46]).In Ubpy silenced fat body cells, double membrane autophagosomes with non-degraded content were present, which is consistent with our previous results showing a blockade of the autophagic flux (Fig 4B and 4C).We further observed significantly smaller lysosomes and numerous small vesicles with homogenous, electron-dense contents corresponding to vesicles budding from the Golgi apparatus and transporting lysosomal hydrolases to late endosomes (Fig 4B -4E).These observations fully support the view that Ubpy loss-of-function in Drosophila fat body cells results in the accumulation of autophagosomes and further indicate morphological defects in lysosomes of Ubpy inactivated cells.
In order to be degraded autophagososmes have to fuse with lysosomes.Thus any disturbance of the function and/or biogenesis of lysosomes could potentially affect autophagy [47].Two classes of proteins are essential for the function of lysosomes: soluble lysosomal hydrolases (also known as acid hydrolases) and integral lysosomal membrane proteins (LMP).One of the most abundant LMP is the lysosomal-associated membrane protein 1 (LAMP1).In wild-type cells, a GFP-LAMP1 fusion protein stained large perinuclear vesicles corresponding to lysosomes as well as smaller vesicles distributed in the cytoplasm (Fig 5A).In cells expressing the dsRNA targeting Ubpy, the large perinuclear lysosomes were missing whereas smaller dots were still present (Fig 5B and 5E).These results thus show that inactivation of Ubpy results in a marked reduction of the size of lysosomes as visualized by the GFP-LAMP1 marker.
As previously indicated lysosomes contain many acid hydrolases which are responsible for their catabolic capacity and cathepsins are important constituents of this lytic system.These enzymes are synthesized in the endoplasmic reticulum, sorted in the Golgi apparatus using the mannose-6-phosphate receptor and delivered to late endosomes [48].In starved wild-type larvae, Cathepsin L staining identifies large lysosomes (Fig 5C).In contrast, cells expressing the dsRNA targeting Ubpy showed a drastic change in Cathepsin L distribution: the overall staining intensity was decreased (Fig 5D and 5F), the large lysosomes were missing and only dots presumably corresponding to vesicles transporting Cathepsin L from the Golgi apparatus were present.Combined with our previous data, these results thus indicate that inactivation of Ubpy strongly affects lysosomal morphology and/or biogenesis.

Inactivation of human UBPY in HeLa cells activates autophagy
In human cells, UBPY has been shown to regulate mitophagy (elimination of damaged mitochondria by autophagy) by controlling Parkin recruitment to depolarized mitochondria after CCCP treatment [22].However, its role in basal autophagy has not been assessed.We first overexpressed the wild-type UBPY protein and its catalytically inactive, dominant negative mutant form [32] in HeLa cells stably expressing the GFP-LC3 autophagy reporter [49].Whereas overexpression of wild-type UBPY had no effect, overexpression of its catalytically inactive mutant form significantly increased the number of autophagosomes per cell (Fig 6A We next asked whether autophagosomes accumulation in UBPY knock-down cells is the result of de novo autophagosome formation due to autophagy induction or of inhibition of their degradation blocking the autophagy flux.First, we analyzed autophagy in the presence of bafilomycin A1 (BAF), an inhibitor of autolysosome acidification.We found that BAF treatment further increased the number of GFP-LC3 dots observed in shUBPY expressing cells as  This result suggests that the observed accumulation of autophagosomes in UBPY knocked down cells is not due to a full autophagy flux blockade.We further observed that the levels of the autophagy target protein p62 were not different in shUBPY expressing cells compared to control cells (Fig 6D and 6E) which also indicates that the autophagy flux is not stalled in UBPY knocked down cells.Finally, we used HeLa cells stably expressing the mRFP-GFP-LC3 reporter that allows for the distinction between autophagosomes (GFP + RFP + ) and autolysosomes (GFP − RFP + ) due to the quenching of the GFP signal in acidic compartments [50].We established mRFP-GFP-LC3 HeLa cell lines stably expressing either the control shNon Target shRNA or the shUBPY #35 shRNA.In keeping with our previous results we found an increased number of total autophagy vesicles in UBPY inactivated cells or in control cells treated with bafilomycin A1 (S5 Fig) .Furthermore, we observed an equivalent ratio of autophagosomes and autolysosomes in shNon Target and shUBPY expressing cells, which is in contrast with the increased proportion of autophagosomes to autolysosomes detected in bafilomycin A1 treated control cells (Fig 6F).Taken altogether, these results show that UBPY knock-down in HeLa cells results in a deregulation of the autophagy flux.

Discussion
In order to identify new DUBs involved autophagy, the 25 DUBs of the USP and UCH subfamilies were screened by RNAi for the induction of an autophagic phenotype in the Drosophila larval fat body.Uch-L3, Usp45, Usp12 and Ubpy were identified in this screen and additional experiments demonstrated that only Usp12 and Ubpy are involved in autophagy in a cellautonomous manner while the two other DUBs may regulate autophagy at the systemic level.Focusing on Ubpy, we have shown that its silencing using two different RNAi transgenes as well as the expression of a dominant negative UBPY protein blocked the autophagy flux.Ultrastructural analysis of Ubpy mutant fat bodies confirmed the presence of autophagosomes with non-degraded contents and further showed that lysosomes were significantly smaller than those observed in wild-type cells.We then looked at lysosomal markers such as the lysosomal membrane protein LAMP1 and the lysosomal hydrolase Cathepsin L and observed that Ubpy silencing resulted in severe lysosomal defects.Taken altogether, these results show that in the Drosophila fat body, UBPY is required for lysosomal biogenesis and/or maintenance and strongly suggest that, as a consequence, the lysosomal defects induced by Ubpy silencing affects autophagic degradation.We have then asked whether the human UBPY protein plays a role in the regulation of basal autophagy.We have observed that overexpression of a dominant negative human UBPY protein or silencing of UBPY in HeLa cells also affected autophagy but by actvating it rather than by blocking the autophagy flux as observed in Drosophila.
In light of the different results obtained in human cells and Drosophila, we cannot rule out the possibility that in Drosophila, UBPY silencing results in both autophagy activation and degradation defects (with the latter being the only observable one) whereas in HeLa cells, UBPY silencing did not result in a full blockade of autophagy, allowing to detect an additional role in autophagy regulation.Alternatively, as the DUB complement of mammals is much more important than the one of Drosophila (approximately 100 DUBs in the human genome [15,16] versus 41 in the Drosophila genome [17]), some redundancy may exist and another human DUB may fulfill its function in lysosomal biogenesis.
In human, in addition to its role in endocytosis UBPY has been shown to regulate mitophagy by controlling Parkin recruitment to depolarized mitochondria after CCCP treatment [22].We show here that UBPY silencing also activates autophagy in absence of CCCP treatment, which strongly suggests that UBPY is not connected exclusively to mitophagy.Additional experiments will be necessary to thoroughly characterize the role of UBPY in this process.
UBPY is mainly known to act in endocytosis.In Drosophila, it controls the stability of the ESCRT-0 subunit Hrs and deubiquitinates cargo of the endocytic pathway.Moreover, its inactivation results in major defects in the endocytic pathway with the accumulation of enlarged endosomes enriched in signaling molecules [33].It has also been shown to regulate the intracellular trafficking of signaling molecules of the Hedghog and Frizzled pathways [32,45,51].A tempting hypothesis is that the defects in lysosomes biogenesis and/or maintenance that we have observed in Ubpy-depleted fat body cells, are related to these defects in the endocytic pathway.Alternatively, UBPY has also been shown to deubiquitinate proteins which are not part of the endocytic machinery such as the TDP-43 and CLOCK proteins [52,53].It is possible that UBPY has additional, yet unknown substrates that would account for the lysososomal defects observed in Ubpy mutant cells.Our work thus open new avenues to the different roles of UBPY and future work will be needed to get a clear understanding of UBPY functions.

Drosophila stocks and clonal analysis
Flies were reared at 25°C on standard cornmeal-yeast medium.The RNAi transgenes targeting the DUBs were obtained from the Vienna Drosophila Resource Center.The second independant RNAi line targeting Ubpy was obtained from Dr. Goto [32].The UAS-2xFlag-UBPY strains were obtained from Dr. Jia [45].The UAS-Luc-IR and UAS-GFP-mCherry-Atg8a (#37749) strains were obtained from the Bloomington Drosophila Stock Center.The UAS-GF-P-Atg8a and UAS-GFP-LC3Blines were obtained from Dr. T. Neufeld and Dr. H. Stenmark, respectively.For the FLPout Gal4/UAS method, spontaneous activation of the Gal4 transcription factor has been reported and allows for the induction of Gal4 expressing cells without heat shock [31].

Immunocytochemistry and microscopy
Antibody and phalloidin stainings were performed as described previously [25].The samples were imaged with a 63x magnification (oil immersion) using a Leica TCS-SP2 confocal microscope and the LCS software.The primary antibodies used in this study were the following: rabbit polyclonal against D. melanogaster Ref(2)P protein [54], mouse monoclonal against Flag tag (Clone M2, Sigma-Aldrich) and rabbit monoclonal anti-Cathepsin L (ab133641, Abcam).The appropriate Cy3-conjugated secondary antibodies were purchased from Jackson Immunoresearch Laboratories.
Lysotracker staining on tissue was performed as in ref. [55].Images were obtained with a fluorescence microscope (Nikon Eclipse 90i) controlled by Nikon Software (Universal Imaging Corp.) using a 60x Plan-Neofluor oil objective.

Electron microscopy
Fed or starved mid-third instar larvae were dissected in PBS.The inverted carcasses were fixed for 2h at room temperature in 2% paraformaldehyde, 0.2% glutaraldehyde in 0.1M cacodylate buffer pH 7.2, rinsed in 0.1M cacodylate buffer, and post-fixed in 1% OsO4, 1.5% potassium ferrocyanide in 0.1M cacodylate buffer for 1h at 4°C.After washes in water, post-staining was done using 5% uranyl-acetate in water for 1h at room temperature in the dark.Carcasses were then dehydrated in graded ethanol series, and dissected fat bodies were embedded in EMBed812 (EMS, 14120), 0,2% DMP30.Ultrathin sections (80nm) were cut using a Leica UC7 ultra-microtome and DiATOME 35°diamond knife and collected on formvar carbon coated 100mesh grids.Sections were stained in 5% uranyl acetate (in water) for 5 minutes and in 2% lead citrate for 5 additional minutes.Images were taken with a CM12 Philips electron microscope at 120 kV using an ORIUS SC1000 CCD camera (Gatan).

Starvation experiments
Feeding larvae were washed twice in PBS and starved for 4 hours on 20% sucrose as an aminoacids-deficient starvation medium [26].
Cells were seeded in 96-well plates (#655090, Greiner) at 12000 cells per well in RPMI.After 18 hours, the medium of each well was replaced with RPMI containing 8μg/ml polybren (Sigma-Aldrich).Lentiviral particles were added at a M.O.I of 5 and 72 hours later, 1μg/ml puromycin (Sigma-Aldrich) was added.
The image acquisitions were performed on an automated microscope ArrayScan VTI (Thermo Scientific) using a Zeiss 20x Plan-Neofluor air objective.Ten fields (corresponding approximately to 1000 cells) were systematically acquired for each fluorescent channels.
Quantification of autophagosomes and autolysosomes was made using to the SpotDetector Bio-Application of Thermo Scientific HCS Studio v6.5.0.Briefly, each nucleus was detected in the Hoechst channel.GFP negative cells were eliminated from the GFP-LC3 channel and cytoplasmic GFP + dots were counted allowing the extraction of the GFP-LC3 dots/cell parameter in the case of GFP-LC3 HeLa cell line.For the mRFP-GFP-LC3 HeLa cells, once the nuclei mask was obtained, RFP+ dots were counted first.Then the RFP mask was applied to the GFP channel allowing for the distinction between autophagosomes which are GFP + RFP + and autolysosomes which are GFP -RFP + .The results were the mean of three different experiments performed in triplicates.

Statistical analysis
Statistical analyses were performed using Prism 6 (GraphPad).For the comparison of two groups, t test has been used.To compare three or more groups, one-way ANOVA with the Dunnett's test for multiple comparisons have been used.

Fig 2 .
Fig 2. Ubpy loss-of-function blocks the autophagy flux.(A,B) Analysis of the autophagy flux using the tandem-tagged GFP-mCherry-Atg8a reporter in control cells from starved larvae (A) or in Ubpy silenced cells (B).Insets show an enlarged view for each condition (arrow: autophagosome, arrowhead: autolysosomes).Quantification of the colocalization of mCherry and GFP signals using the Pearson's correlation coefficient is shown in F. (C,D) Lysotracker Red staining on fat bodies from fed (C) or starved (D) larvae silenced for Ubpy.Mutant cells were identified by the expression of the GFP-Atg8a marker (dotted lines and green channel in insets).(E) Confocal sections of larval fat bodies stained for the endogenous Ref(2)P protein.Mutant cells were identified by the expression of the GFP-Atg8a marker (dotted lines and green channel in insets).(G)

Fig 6 .
Fig 6.UBPY silencing in HeLa cells activates autophagy.(A) The number of GFP-LC3 dots per cell was quantified in HeLa cells stably expressing the autophagy reporter GFP-LC3; cells were transfected with a control plasmid (pME-Flag) or plasmids expressing either the wild-type human UBPY protein (pME-UBPY WT ) or its catalytically inactive mutant (pME-UBPY C>S ).Bars denote mean ± s.d.Statistical significance was determined using t-test: ****p<0.0001(B) The expression of UBPY was monitored by Western blot in GFP-LC3 HeLa cells stably transfected with a control shRNA or three different shRNAs targeting UBPY.(C) The number of GFP-LC3 dots per cell was quantified in GFP-LC3 HeLa cells stably transfected with a control shRNA or three different shRNAs targeting UBPY in absence (black bars) or in presence of bafilomycin A1 (BAF, gray bars).Bars denote mean ± s.d.Statistical significance was determined using t-test: ****p<0.0001;***p<0.005(D) The expression of the autophagy target protein p62 was monitored by Western blot in GFP-LC3 HeLa cells stably transfected with a control shRNA or three different shRNAs targeting UBPY.(E) Quantification of p62 levels in GFP-LC3 HeLa cells stably transfected with a control shRNA or three different shRNAs targeting UBPY from three independent Western blots.(F) The repartition of autolysosmes and autophagosomes was determined in mRFP-GFP-LC3 HeLa cells stably expressing either the control shRNA or the shUBPY #35 shRNA, in comparison with control transfected cells treated with bafilomycin A1. doi:10.1371/journal.pone.0143078.g006