Distinct Phylogeographic Structures of Wild Radish (Raphanus sativus L. var. raphanistroides Makino) in Japan

Coastal plants with simple linear distribution ranges along coastlines provide a suitable system for improving our understanding of patterns of intra-specific distributional history and genetic variation. Due to the combination of high seed longevity and high dispersibility of seeds via seawater, we hypothesized that wild radish would poorly represent phylogeographic structure at the local scale. On the other hand, we also hypothesized that wild radish populations might be geographically differentiated, as has been exhibited by their considerable phenotypic variations along the islands of Japan. We conducted nuclear DNA microsatellite loci and chloroplast DNA haplotype analyses for 486 samples and 144 samples, respectively, from 18 populations to investigate the phylogeographic structure of wild radish in Japan. Cluster analysis supported the existence of differential genetic structures between the Ryukyu Islands and mainland Japan populations. A significant strong pattern of isolation by distance and significant evidence of a recent bottleneck were detected. The chloroplast marker analysis resulted in the generation of eight haplotypes, of which two haplotypes (A and B) were broadly distributed in most wild radish populations. High levels of variation in microsatellite loci were identified, whereas cpDNA displayed low levels of genetic diversity within populations. Our results indicate that the Kuroshio Current would have contributed to the sculpting of the phylogeographic structure by shaping genetic gaps between isolated populations. In addition, the Tokara Strait would have created a geographic barrier between the Ryukyu Islands and mainland Japan. Finally, extant habitat disturbances (coastal erosion), migration patterns (linear expansion), and geographic characteristics (small islands and sea currents) have influenced the expansion and historical population dynamics of wild radish. Our study is the first to record the robust phylogeographic structure in wild radish between the Ryukyu Islands and mainland Japan, and might provide new insight into the genetic differentiation of coastal plants across islands.


Introduction
Coastal plants offer several advantages as a suitable system for improving our understanding of patterns of intra-specific distributional history and genetic variation [1]. First, coastal plants are often widely distributed, covering large geographic ranges latitudinally and longitudinally, often containing both refugial and recolonized areas. Second, they essentially have linear distribution ranges along coastlines, which limit the spatial options for migration and facilitate the reconstruction of distributional limits in the Quaternary glacial period [1,2]. These advantages characterize coastal plants as a suitable system for inferring distribution history and assessing the effects of historical and extant factors on geographic patterns of genetic variation. Increasing investigations of the geographic distribution of intra-specific genetic variation in a large number of coastal plants have resulted in the detection of the presence or absence of phylogeographic structures at large and local scales.
Investigations of the geographic distribution of intra-specific genetic variation in coastal plants have often resulted in the detection of clear phylogeographic structures; e.g., Hordeum marinum [3], Triglochin maritima [4], Eryngium maritimum [5], and Carex extensa [6] in Mediterranean and European coasts, Zostera marina in Northern Hemisphere coasts [7], and Hibiscus tiliaceus in Pacific and Indian Ocean regions [8]. These patterns were primarily explained by historical processes (e.g., Pleistocene glaciations). Independent colonization was suggested to have occurred in survival populations where suitable habitats were available during Pleistocene glaciations, which in turn influenced the geographic distribution of species by range expansion during the climate amelioration, companied by shaping distinct phylogeographic structures. Some authors have focused on sea currents, which can enable gene flow over a wide range for coastal plants with sea-drifted seeds and constitute a barrier or transport route for seeds or fruits, and thus have strong impacts on geographic patterns of genetic variation [8,9]. Additionally, some authors have proposed the influence of sea straits as barriers to gene flow contributing to phylogeographic structures [9]. During the last glacial maximum (LGM; ca. 20,000-18,000 BP), the low sea level may have resulted in the drying out of connections between sea basins, restricting dispersal routes of propagules and isolating populations from each other [9]. The absence of phylogeographic structures has rarely been reported [2,10]. Factors related to specific properties of species, such as clonal growth and/or long-distance dispersal, have been proposed to account for colonization success.
In contrast to the large scale, coastal plants have frequently been reported to lack or have an obscure phylogeographic structure at local scales; e.g., Calystegia soldanella (in Europe, Korea, and Japan) [10][11][12], Lathyrus japonicus (Japan) [13], Suaeda maritima (Europe) [14], Uniola paniculata (Southeastern United States) [15], and Carex arenaria (Europe) [16]. The most likely contributing factor was that seeds of coastal plants can be frequently dispersed by sea currents and can survive for long periods [17], which can either erase or prevent the sculpture of the historical phylogeographic structure [10]. However, former studies have reported distinct phylogeographic structures of the coastal plants Ophiorrhiza japonica [18] and Farfugium japonicum [19] in the Ryukyu Islands. They explained that the past splitting of a land bridge (straits) would have influenced population structures by limiting their geographic range during the Pleistocene climate oscillations.
One coastal plant, wild radish, classified as Raphanus sativus L. var. raphanistroides Makino (Brassicaceae), is found most commonly on sandy coasts or estuaries and occasionally inland along riverbanks in eastern Asia. Wild radish is characterized by seawater-dispersed seeds surrounded by a water-impermeable seed coat and a large air-filled cavity (spongy pericarp) (S1 Fig). The seeds have been reported to float in seawater and then remain in the seed bank for a prolonged period, during which they are covered by sand, until germination [17]. Thus, wild radish was expected to lack phylogeographic structure as the result of the high potential for frequent seed dispersal at the local scale, such as on the islands of Japan. Indeed, wild radish in Japan was reported to harbor no phylogeographic structure based on allozyme polymorphisms [20], amplification fragment length polymorphism (AFLP) analysis [21], and chloroplast (cp) DNA haplotypes [22]. On the other hand, considerable phenotypic variations among local populations of wild radish in Japan can be identified in our personal observations (S2 Fig). Wild radish plants in the Ryukyu Islands tend to have glabrous leaves and stems, whereas those of mainland Japan are covered by dense setose trichomes. It seems plausible to postulate that the morphological differentiations of wild radish in isolated regions are accompanied by geographical distributions of genetic variation, that is, local populations may be genetically isolated from each other and finally present phylogeographic patterns at a local scale in Japan. Based on this background information, understanding phylogeographic structures would be helpful in providing new insight into the geographical genetic variation of coastal plants on the islands of Japan.
In the present study, we hypothesized the equal possibilities that (a) high frequency gene flow via seed dispersal would decay the phylogeographical structure of the coastal populations on the Japanese islands, or (b) any geographic and/or inorganic factors may sculpt the genetic structure of wild radish, as has been suggested by intra-specific variations of phenotypes. In addition, past population demography, including the traces of bottleneck, range shift histories, and genetic variation of the extant populations, were examined. For these objectives, nuclear DNA microsatellite loci (nuclear simple sequence repeats; nSSRs), and cpDNA haplotypes were used to evaluate the phylogenetic relationships among wild radish populations covering most of the geographic ranges in Japan.

Sampling and DNA extraction
We did not require any specific permission to enter our sampling locations, and we confirmed that our field studies did not compromise endangered or protected species. Leaf material from wild radish was collected from 18 natural populations, covering most of the geographic ranges in Japan, including the Ryukyu Islands, Kyushu Islands, Shikoku Islands, Honshu Islands, and Hokkaido Islands. Details of the locations of populations and the numbers of samples are provided in Fig 1 and Table 1. To avoid sampling close relatives within individual populations, we randomly collected foliar samples at intervals of 10-50 m along the coastlines, with the exception of the population of Shiga (Pop. 10). In this area, the minimum interval was only 3 m because the population was of limited size (as its status was "vulnerable" on the shore of the freshwater Lake Biwa). The sampled leaves were placed immediately in drying silica gel in the field, returned to the laboratory, and stored at 4°C. Dried leaf materials were pulverized into fine powder. Following removal of polysaccharides from the samples using 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (HEPES; pH 8.0) [23], DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method [24]. Extracted DNA was dissolved in 100 μL Tris-EDTA (TE) buffer and subjected to amplification by polymerase chain reaction (PCR).

Microsatellite genotyping and data analyses
Nine microsatellite markers were selected based on their clarity and reproducibility [27][28][29] (S1 Table). PCR amplification was performed in a final volume of 5 μL (containing 40-60 ng genomic DNA) following the standard protocol of the Qiagen Multiplex PCR kit (Qiagen, Hilden, Germany). Compound SSR primers [(AC) 6 (AG) 5 , (TC) 6 (AG) 5 , or (GA) 5 (CA) 5 ] were labeled with the fluorochrome 6-FAM or HEX (Applied Biosystems, Foster City, CA. USA). Amplification was performed using an initial denaturation step of 15 min at 95°C, followed by 32 cycles of denaturation for 30 s at 95°C, annealing for 90 s at 58°C, and extension for 30 s at 60°C, with a final elongation step at 72°C for 10 min. Amplified products were loaded onto an ABI 3130 autosequencer (Applied Biosystems) using the GeneScan Rox-350 Size Standard (Applied Biosystems) and the POP7 polymer and a 36-cm capillary array, and their sizes were determined using GeneMapper (Applied Biosystems). Departures from Hardy-Weinberg equilibrium (HWE) at each locus were carefully explored using a Markov chain Monte Carlo (MCMC) method implemented in GENEPOP version 4.0.10 [30,31]. MICRO-CHECKER [32] was employed to estimate the most probable cause of any departure from HWE, including the presence of null alleles, scoring errors caused by stuttering, or allelic dropout attributable to short-allele dominance. Approximately 10% of all samples were amplified and genotyped at least twice; the rate of genotyping error was <5%.  Table 1. The square box indicates the Tokara Strait region and is modified from the graphic of Feng et al. [25]. The main path of the Kuroshio Current, modified from the graphic of Yin et al., is also shown [26].
A model-based Bayesian clustering method was applied to assign individuals to populations using the STRUCTURE version 2.3 software [33]. This method identifies K (unknown) populations within a dataset and assigns each population/individual to one or more populations/clusters if the individual is admixed. Markov chain Monte Carlo (MCMC) values were set for a burn-in period of 30,000 and a run length of 10 5 iterations under an admixture model with correlated allele frequencies within populations. The batch run function was carried out for a total of 400 runs (20 runs each for 1-20 clusters; i.e., K = 1-20) to quantify the variation of the likelihood of each K value. To obtain the correct estimate of the number of clusters, the rate of change of the log probability (ΔK) between successive values of K was evaluated using the method of Evanno et al. [34]. Then, 400 runs of the simulation with the highest modal value of ΔK were aligned by the cluster matching and permutation software CLUMPP version 1.1 [35] and presented as bar graphs using DISTRUCT version 1.1 [36].
To assess the genetic diversity of each locus and each population, observed heterozygosity (H O ), Nei's gene diversity (expected heterozygosity H E ) [37], inbreeding coefficient (F IS = 1-H O /H E ) [38], and population differentiation based on pairwise F ST between populations were evaluated using the GENALEX version 6.5 software [39]. The frequency of null alleles (Na) [40] was calculated using the formula: (H E -H O )/(H E +H O ) (per locus across all populations). The number of alleles per population was calculated using FSTAT version 2.9.3 [41].  [43]. Bootstrapping based on microsatellite data was conducted using 1000 replicates.
To detect recent bottlenecks caused by reductions in effective population size, the observed gene diversity was compared with the equilibrium gene diversity given the observed number of alleles [44,45] using BOTTLENECK version 1.2.02 [46]. Two models of locus evolution, the infinite allele model (IAM) [47] and the stepwise mutation model (SMM) [48], were used for the analyses together with the sign test [48] and the Bayesian Wilcoxon signed-rank test [49].
The association between pairwise estimates of population differentiation (F ST /1-F ST ) and the natural logarithm of the corresponding geographic distance was estimated using the Mantel test [50] in GENALEX version 6.5, with significance tested using 1000 permutations.
Chloroplast DNA haplotyping and data analyses PCR amplification of cpDNA was conducted for 144 samples (eight individuals per population) using one universal primer pair (trnL-F), and two internal primer pairs (trnT-L and rpl20-rps12) designed based on published primers [51,52] (S2 Table). PCR amplification was conducted in a total reaction volume of 10 μL containing 7.25-μL autoclaved ion-exchanged water, 0.8-μL 2.5 mM dNTP mixture, 1-μL 10× Ex Taq Buffer (Takara Ex Taq; Takara, Kusatsu, Japan), 0.25 U Ex Taq (Takara), 0.2 μM of each primer, and 0.5-μL DNA. Amplification was performed using an initial denaturation step for 5 min at 94°C, followed by 35 cycles of denaturation for 1 min at 94°C, annealing for 1 min at 54°C, and extension for 1.5 min at 72°C. Following amplification, the products were visualized on 0.5%-TAE agarose gels stained with ethidium bromide. PCR products were sequenced using the standard methods of the Big-Dye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) with the primers specified in S2 Table on an ABI Model 3100 Genetic Analyzer (Applied Biosystems). All sequence data were analyzed and aligned using Auto Assembler (Applied Biosystems). Basic sequence statistics, including haplotype diversity (h) and nucleotide diversity (π), were calculated using the DnaSP version 5.10 software [53]. CpDNA haplotypes were determined based on the aligned sequences, and a parsimony network was constructed using the TCS version 1.21 software [54].

Microsatellite diversity and structure
The gene diversity parameters for the nSSRs are shown in Table 1 Table). With the exception of RsHR026 and RsSA085, the other seven loci exhibited significant deviations from HWE (S1 Table). MICRO-CHECKER analysis suggested that null alleles were present at all nine loci (P <0.05), as were scoring errors caused by stuttering (with the exception of RsSA085). However, no evidence of large-scale allele dropout was obtained for any locus (P >0.05). The presence of null alleles increases the numbers of homozygotes. Similar degrees of heterozygotic deficiency were evident in Japanese and Korean wild radish populations subjected to analysis of microsatellite [27] and allozyme variations [21]. As the wild radish is self-incompatible, any excess of homozygotes is attributable to the genetic substructure of a local population, in which either the Wahlund effect and/or biparental inbreeding may be in play. Under the SMM, five populations (pops. 4, 7, 11, 12, and 16) exhibited significant heterozygosity excess (i.e., evidence of a recent bottleneck) by the sign test and nine populations (pops. 2, 3, 4, 7, 11, 12, 13, 16, and 18) by the Wilcoxon test (S3 Table). A highly positive relationship was observed between pairwise genetic distance and geographic distance over total populations (R 2 = 0.2953, p<0.001; S3 Fig), suggesting a significant isolation by distance (IBD) pattern across all 18 wild radish populations in Japan.
The results of Bayesian clustering analysis indicated that the most likely number of clusters was 2 when the ΔK statistics were applied (ΔK = 2; Fig 2), which was strongly supported by the highest similarity coefficient values (H) using CLUMPP analysis (S4 Fig). The cluster analysis revealed that the Ryukyu Islands populations (pops. 1-4) and mainland populations (pops. 5-18) were genetically distinct (Fig 3). In addition, the similarity coefficient H determined using CLUMPP suggested the other appropriate number of clusters to be K = 3, with the second highest ΔK statistics. Thus, the Ryukyu Islands populations were clearly assigned to independent clusters using the two most appropriate numbers of clusters. The alpha values (alpha is the Dirichlet parameter evaluating the extent of admixture) varied greatly throughout the runs (K = 2 and K = 3) (S5 and S6 Figs), indicating that the [Ln P(D)] values had been accurately estimated for each K [55]. In accordance with the results of Bayesian clustering analysis, two major clusters were identified from NJ phylogeny (S7 Fig) and weakly supported by

Chloroplast DNA diversity and haplotype distribution
Based on approximately 1,375 bp of the three noncoding regions of cpDNA, eight haplotypes were detected in a total of 144 individuals from 18 wild radish populations. The haplotype diversity (h) and nucleotide diversity (π) of cpDNA sequences are shown in Table 1. The haplotype diversity (h) ranged from 0 to 0.786, with an average of 0.347. The nucleotide diversity (π) varied from 0 to 0.343, with an average of 0.00133.
The distribution of the observed eight haplotypes of wild radish is shown as a parsimony network in Fig 1. A cpDNA haplotype network is created by homoplasy that is, in turn, caused by recurrent mutations at the same sites [56] and/or possible recombinations (that have previously been detected in radish cpDNA; [57]). Haplotypes B and A were broadly distributed in wild radish. Haplotype B was the most abundant (38.9%) and widely distributed across the Japanese Archipelago. Haplotype A also ranged across the archipelago (36.8%), but at a lower frequency than that of haplotype B. Specifically, only haplotype D was restricted to Ishigaki Island (pop. 2) of the Ryukyu Islands, whereas haplotypes E, F, G, and H were unique to the mainland populations. Of the 18 populations surveyed, six were dominated by single haplotypes (A and B). With the exception of pop. 2, which exhibited four haplotypes, the Ryukyu Island populations (pops. 1, 3, and 4) were fixed with haplotypes A and/or B, identical to the majority of wild radish populations in mainland Japan.

Population structure
The present study provides the first evidence of clear geographic structure observed in coastal plants between the Ryukyu Islands and the adjacent mainland Japan; wild radish populations in northern and southern Japan were clearly demarcated around Yakushima Island (the northernmost area of Ryukyu Islands) based on microsatellite variation. This distinct phylogeographic structure is likely attributable to two major causes. First, sea currents are proposed to influence the phylogeographic structure of wild radish in Japan by shaping barriers and transport of seed dispersal. The present Kuroshio Current originates from the North Equatorial Current in the West Pacific, and flows along the Ryukyu Islands towards the north. Then it moves eastward, finally reaching the southern sea of the main islands of Japan through the Tokara Strait near Yakushima Island (Fig 1) [58,59]. Considering the high dispersibility of  Table 1. seeds via seawater in coastal plants, the Kuroshio Current is proposed to play two contrasting roles on the phylogeographic structure of wild radish. On one hand, the Kuroshio Current promotes gene exchanges among the isolated Ryukyu Islands by transporting spongy fruits of wild radish, and induces uniformity in their genetic structure across the Ryukyu Islands, which stretch over 1,000 km. On the other hand, it also acts as a geographic barrier against seed dispersal and in turn shapes the genetic gap between the Ryukyu Islands and mainland Japan.
The Kuroshio Current effect is also likely responsible for the genetic admixtures between several mainland populations (pops. 8 and 9) and Ryukyu Islands populations. Pops. 8 (in Mie) and 9 (in Kanagawa) are greatly distant from the Ryukyu Islands but are located downstream of the Kuroshio Current, which flows from Ryukyu Islands areas. Accordingly, seeds of wild radish are unavoidably and limitedly transported from the Ryukyu Islands to these populations, promoting gene exchange between these regions. This finding coincides with a previous study that reported genetic similarity in wild radish among one insular population of the Ryukyu Islands and several populations from the western part of the Pacific coastal area (near Mie prefecture) based on the allozyme diversity [20]. In general, sea currents strongly affect geographic genetic structure in coastal species by constituting a barrier or transport mechanism for seeds or fruits [1,8,12,60]. Distinctly and originally, the present study recorded the two contrasting effects of the Kuroshio Current functioning simultaneously on the coastal plants, which might provide new insight into genetic differentiation across the islands of Japan. On the other hand, phenotypes of the wild radish (trichomes and length of hypocotyl) in the western part of the Pacific coastal area are apparently the same as those of mainland Japan wild radish (dense setose in leaves and stems) (personal observation), suggesting that seed migration and settlement are rare.
Second, the Tokara Strait was thought to have created a boundary between the Ryukyu Islands and mainland Japan. The Tokara Strait is located between Amamioshima and Tanegashima Islands, south of Kyushu (Fig 1) [25], and has persisted since its formation in the Pliocene, ca. 2-5 Ma [61]. During the glacial period, ocean water levels are estimated to have been more than 100 m lower. However, the Tokara Strait still existed because the water depth was more than 1,000 m. As a result, the migration of species north and south of the Tokara Strait is assumed to have been blocked, and thus it formed a borderline [18,62]. Therefore, the Tokara Strait can be assumed to have been a geographic barrier, which may ultimately have resulted in the genetic divergence between the Ryukyu Islands and mainland Japan populations of wild radish. Considering the splitting of the land bridge during the LGM period, plants in both the Ryukyu Islands and mainland Japan were supposed to have experienced different migration and recolonization histories [63], and in turn, vicariance events likely occurred. Thus, it is supposed that the Tokara Strait had a significant influence on the distribution of wild radish, shaping the geographic distribution of genetic variation in the Ryukyu Islands and mainland Japan. In addition, the Tokara Strait is the main pathway of the Kuroshio Current when it moves eastward, thus the Tokara Strait and Kuroshio Current work together to shape a robust barrier against migration between the Ryukyu Islands and mainland Japan.
In contrast to the clear phylogeographic structure detected by microsatellite loci, no geographic distribution pattern of haplotypes was observed in cpDNA variations. The discordance is often associated with the different inheritance modes and evolutionary rates between nuclear and chloroplast genomes. Nuclear genes are biparentally inherited and dispersed, whereas the chloroplast genes are inherited predominantly or entirely from the female parent. They segregate rapidly during vegetative growth, so the low heterozygosity in chloroplast genes is feasibly shaped without recombination [64]. This would also be the contributing factor for the low levels of cpDNA variation in terms of nucleotide and haplotype diversity within populations of wild radish ( Table 1). The other possible main reason would be the low evolutionary rate of cpDNA. Previous studies that compared the rate of substitution both at synonymous sites and in noncoding sequences have suggested that chloroplast genes have lower rates of nucleotide substitution (only half of the nuclear DNA evolutionary rate) [65]. Accordingly, cpDNA variation infers a past change in population demographics, such as population expansion or decline, whereas microsatellite variation reflects recent events in the population. Thus, the geographic distribution of cpDNA variation in wild radish in isolated Ryukyu Islands and mainland Japan is assumed to have required time to become established.

Population demography
Evidence that most populations shared the common cpDNA haplotypes (A and B) across the entire Japanese archipelago (Fig 1) suggests a scenario in which wild radish along the archipelago might have derived from a common ancestor, that is, the coalescent effect, which leads to the wide geographic distribution of ancestral haplotypes [66,67]. Namely, the sharing of the predominant haplotypes among all populations may be most plausibly explained by the retention of the ancestral haplotypes. Due to the considerable genetic drift in wild radish populations, some haplotypes have randomly diminished, which likely resulted in some haplotypes being unique to particular populations (e.g., haplotype D was unique to pop. 3). Other possibilities should also be considered: gene flow is likely responsible for the haplotypes common to the total population. A combination of the high frequency of long-distance dispersal via ocean currents and the high longevity of seeds could eventually result in hybridization and/or introgression, although phenotypic variation may depress the reproductive success of immigrants to some extent.
Historical population dynamics of wild radish were reflected by the significant bottleneck effect and IBD pattern. The recent bottleneck in wild radish populations on the islands of Japan is most plausibly explained by frequent submergence caused by the high frequency of habitat disturbances, such as frequent intermittent typhoons, striking wild radish populations. In addition, due to the relatively small geographic size of islands and the past fragmentation during the LGM period, plants in islands show increased sensitivity to genetic drift. The significant correlation between geographical and genetic distances indicates the model of stepwise range expansion in wild radish. It is attributed to the linear arrangement of habitats, with seeds assumed to move in a linear pattern via sea currents, that is, geographically close populations are phylogenetically close. Due to the genetic boundary between the Ryukyu Islands and mainland Japan, migration is restricted or rare, but not impossible, as evidenced from the long floating time and long viability of seeds. In addition, the numerous multidirectional tributaries of sea currents surrounding the Japanese archipelago are also responsible for the expansion of wild radish populations. Our results are consistent with the positive correlation of IBD in wild radish reported by Ohsako et al. [27] using microsatellite loci, as well as in other coastal plants such as Cakile maritima, Salsola kali, and Halimione portulacoides in the Atlantic clusters [1].
Environment factors. Fruits of wild radish comprise a few-seeded capsule with a very solid and water impermeable seed coat containing a large air-filled cavity; these characteristics are essential to enhancing seed buoyancy, promoting seed dispersal by seawater over long distances, and high longevity. Accordingly, sea currents enable frequent gene flow over the wide distribution range of wild radish. However, the Ryukyu Islands and mainland Japan experience different histories and hence produce different selection pressures, and these in turn shape the genetic heterogeneity between local populations by producing different selective forces. Also, these two geographic ranges differ in both abiotic (e.g., temperature and precipitation) and biotic (such as the extensive morphological variation of wild radish) factors, which may generate ecological barriers against gene flow. These two forces work synergistically, producing genetic heterogeneity in natural populations and thus enhance genetic differentiation and preserve the robust phylogeographic structure. This hypothesis does not conflict with the observation of genetic admixture between several mainland populations (pops. 8 and 9) and the Ryukyu Islands, which exhibit great morphological variation, implying that restricted gene flow could not be disturbed by the existing robust geographic pattern of genetic variation. However, abiotic factors (such as vicariance mediated by sea currents) and/or in situ factors could not be determined to be causative, thus it is necessary to perform studies involving reciprocal transplantation and/or sympatric cultivation of wild accessions.

Conclusions
Considering wild radish as a typical coastal plant with high longevity and high dispersibility of seeds via sea currents, it poorly represents phylogeographic structure at local scales. However, our study is the first to record the robust phylogeographic structure in wild radish between the Ryukyu Islands and the adjacent mainland Japan, which might provide new insight into the genetic differentiation of coastal plants across islands. The Kuroshio Current has an important influence on the geographic distribution of genetic variation of wild radish by shaping the genetic gap between isolated populations. In addition, the Tokara Strait is proposed to have been responsible for the genetic isolation between these two clusters. Finally, extant habitat disturbances (coastal erosion), migration patterns (linear expansion), and geographic characteristics (small islands and surrounding sea currents) have influenced the expansion and historical population dynamics of wild radish.