Coordinated Expression of FLOWERING LOCUS T and DORMANCY ASSOCIATED MADS-BOX-Like Genes in Leafy Spurge

Leafy spurge (Euphorbia esula L.) is a noxious perennial weed that produces underground adventitious buds, which are crucial for generating new vegetative shoots following periods of freezing temperatures or exposure to various control measures. It is also capable of flowering and producing seeds, but requires vernalization in some cases. DORMANCY ASSOCIATED MADS-BOX (DAM) genes have been proposed to play a direct role in the transition to winter-induced dormancy and maintenance through regulation of the FLOWERING LOCUS T (FT) gene, which also is likely involved in the vernalization process. To explore the regulation of FT and DAM during dormancy transitions in leafy spurge, the transcript accumulation of two previously cloned DAM splice variants and two different previously cloned FT genes was characterized. Under long-photoperiods (16 h light), both DAM and FT transcripts accumulate in a diurnal manner. Tissue specific expression patterns indicated the tissues with high DAM expression had low FT expression and vice versa. DAM expression is detected in leaves, stems, shoot tips, and crown buds. FT transcripts were detected mainly in leaves and flowers. Under dormancy inducing conditions, DAM and FT genes had an inverse expression pattern. Additionally, chromatin immunoprecipitation assays were performed using DAM-like protein specific antibodies to demonstrate that DAM or related proteins likely bind to cryptic and/or conserved CArG boxes in the promoter regions of FT genes isolated from endodormant crown buds. These results are consistent with the hypothesis that DAM proteins play a crucial role in leafy spurge dormancy transition and maintenance, potentially by negatively regulating the expression of FT.


Introduction
Leafy spurge (Euphorbia esula L.) is an invasive perennial weed that infests range and recreational lands in the great plains of the US and Canada. The perennial nature of leafy spurge is down-regulated upon induction of DAM, which was paralleled by transitions from paradormancy to endo-and eco-dormancy in leafy spurge crown buds [19]. Further, over-expression of leafy spurge DAM genes in arabidopsis had a negative effect on flowering and growth [21]. Likewise, over expressing a Japanese apricot DAM gene in poplar enhances dormancy induction in poplar, and the highest DAM expressing lines had lower levels of FT transcripts than lines showing low DAM expression or which over-expression had little impact on the phenotype [22]. Therefore, because of the structural similarity between DAM and SVP, and the role of FT in controlling bud dormancy in poplar as well as the impact of expression of DAM genes on FT transcript accumulation in heterologous systems, the leafy spurge DAM proteins have been proposed to play a direct role in dormancy induction and maintenance through negative regulation of FT [19]. Subsequently, two genomic BAC clones containing FT-like genes from leafy spurge and several BAC clones containing very similar DAM gene have been cloned and characterized [20].
Here we test the hypothesis that DAM regulates FT expression, and document the expression of DAM and FT genes, including diurnal expression, tissue-specific expression, and the expression pattern under dormancy induction. Results indicate that DAM and FT genes are expressed in a manner consistent with DAM negatively regulating FT expression, but the minimal tissue-specific expression of FT in the buds along with minimal differential expression of DAM in the leaves suggests that modification of the hypothesis may be in order. Additionally, we examine the possible interaction of DAM with FT promoter elements using chromatin immunoprecipitation assays using an antibody proven to interact with the leafy spurge DAM protein(s).

Plant materials and treatments
Three-month-old leafy spurge plants, grown in sunshine mix in 1-× 8-inch conetainers under 16 h of natural and supplemental lighting (from 6:00 AM to 10:00 PM CST) and an average greenhouse temperature of approximately 25°C, were used in this study. For tissue-specific gene expression analysis, different organs were collected separately at 9:00 PM and frozen immediately in liquid N 2 . Every sample was harvested from five individual plants for each of three biological replicates. For diurnal gene expression analysis, mature leaves for each sample were collected from three individual plants every 4 hours started at 2:00 AM and continuing for three consecutive days. All samples were immediately frozen in liquid N 2 and stored at -80 C. For dormancy induction analysis, plants were moved into a growth chamber under long-photoperiod (16 hrs) with cold night, morning, and evening (11 C for 16 hrs) but warm mid-day (24 C for 8 hrs) conditions. These conditions have been shown to induce endodormancy within 6 weeks [21]. Samples were collected at 0-8 days, and 1.5 through 7 weeks. Shoot apices and leaves were collected for expression analysis during the first four weeks of dormancy inducing conditions when crown buds were small. After that, leaves and crown buds were collected because aboveground parts stopped growing but crown buds were enlarged. Two biological replications were collected. Samples were frozen in liquid N 2 immediately after collection and stored at −80°C for further study. After 7 weeks of treatment to induce endodormancy, plants were exposed to 5°C and short-photoperiod (8 hours) to vernalize and induce ecodormancy. After four weeks, we removed all aerial tissues and moved these plants into a greenhouse to check dormancy status (the plants with 20 weeks vernalization were also examined to confirm they had broken endodormancy). The length of the longest shoots from crown buds of at least 7 plants were measured after two weeks.

RNA extraction and expression analysis
Frozen samples were ground in liquid N 2 and RNAs were extracted following the pine tree extraction protocol [23]. The concentration of RNA was measured using a NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, USA), and the quantity and integrity was determined by gel electrophoresis. RNA (5 μg) was first treated with DNase I (Invitrogen #18068-015) and then used for first-strand cDNA synthesis (Invitrogen #18080-051). qRT-PCR was performed using cDNA templates on an LightCycler 480 II (Roche, USA) Real-Time PCR system following the methods of Chao et al. [24]. Gene specific primers (S1 Table) were designed for quantifying transcript abundances, and the SAND gene (gene ID: CV03067B2A12.f1) was used as an internal control. SAND gene was verified to be stably expressed during bud development [24].

DAM antibody preparation and immunoblot analysis
A conserved peptide (RRGLFKKAHELS) was designed for antibody preparation based on protein sequences generated from leafy spurge DAM1 and DAM2 (gene ID: EU339320.1). Peptide synthesis, antibody production, and affinity purification were carried out by Affinity Bioreagents (Golden, CO) according to the company's general protocols. A pGEX-DAM fusion protein was generated for immunoblot analysis. pGEX-DAM fusion construct, protein overexpression, and affinity purification were performed following the procedures described [25]. Immunoblot analysis was done as previously described [26].

Diurnal expression of DAM and FT genes in leafy spurge
DAM and FT expression has not been well characterized in leafy spurge. To determine the relationship between them we first investigated their diurnal and tissue-specific expression patterns under long-photoperiod conditions for three consecutive days (Fig 1). The results are consistent with both DAM transcripts having a diurnal expression patterns that peak between 2:00 PM and10:00 PM in the evening-prior to the dark period ( Fig 1A). After that time point, accumulation of the two DAM transcripts decreased significantly throughout the dark phase and reached the lowest expression levels in at or about the time of daybreak (6 AM).
The expression level of both leafy spurge FT2 (gene ID: JX966353.1) and FT4 (gene ID: JX966355.1) genes started to increase at 6:00 AM in the morning, and reached a peak between 6:00 PM and 2:00 AM ( Fig 1B). After that the expression of FT genes decreased dramatically and reached the lowest point in the morning at the onset of the light period.

Tissue-specific accumulation of DAM and FT transcripts in leafy spurge
The transcript levels for DAM and FT in different organs were detected at the end of the 16 hour photoperiod. The tissue specificity data indicated that transcript abundance for both DAM1 and DAM2 was relatively greater in leaves, stems, shoots and crown buds, and was less abundant in flowers (Fig 2). DAM1 particularly accumulated mostly in shoots and crown buds and, in roots, transcript abundance for DAM1 was significantly greater than that of DAM2 ( Fig  2A). Both FT2 and FT4 genes were preferentially expressed in leaves and flowers, and transcript abundance of FT2 is greater than that of FT4 in all measured tissues ( Fig 2B).

Long photoperiods with cold evening nights and mornings can induce endodormancy and vernalization increases growth potential of crown buds
We confirmed that the treatment of long photoperiods with cold evenings, nights, and mornings would induce endodormancy, and that extended cold would not only release the plants from endodormancy, but increase the growth potential of the crown buds (Fig 3). After the decapitated plants with different treatments (long photoperiod no cold-paradormant, 4 weeks or 7 weeks of long photoperiod with cold evenings, nights and mornings-endodormant, and endodormant-inducing conditions followed by 20 weeks of short photoperiod cold days and nights-ecodormant/vernalized) were moved to greenhouse, the regrowth from crown buds was measured after 2 weeks (Fig 3). Regrowth was significantly suppressed from plants receiving four to seven weeks of endodormancy-inducing conditions relative to either paradormant buds that had received no cold treatment. In contrast, shoot growth from crown buds increased significantly after twenty weeks vernalization treatment and 100% of these plants flowered after three weeks in green house (data not shown). These results confirmed earlier studies that consistent long photoperiod with cold evenings, nights, and mornings caused adventitious buds to transition from paradormancy to endodormancy, whereas twenty week cold treatments resulted in a transition to ecodormancy and maximal growth competency.

Correlating DAM and FT expression during endodormancy inducing and releasing conditions
To identify the relationship in transcript abundance between DAM and FT gene during endodormancy induction, the transcript levels of DAM and FT were detected in leaves and shoot apices for seven weeks under endodormancy-inducing conditions (Fig 4). The expression data indicated that, DAM1 and DAM2 had similar accumulation patterns (Fig 4A and 4B); both DAM1 and DAM2 transcripts increased slightly but significantly during the first day, but increased substantially in shoot tissue between 8 and 15 d of treatment. However, by week 4 the shoot tips had ceased growing and were greatly reduced in size and leaf tissue had senesced. In crown buds (which were too small to sample early in the dormancy process), the levels of DAM transcripts increased sharply from the fourth week when crown buds were first collected  FT2 and FT4 also had very similar transcript accumulation patterns (Fig 4C and 4D). Interestingly, accumulation of both FT transcripts was clearly suppressed in leaves after one week cold treatment, which was coordinately paralleled by increased DAM transcript accumulation in the shoot apices. Additionally, FT2 transcripts were detected in shoot apices/crown buds at low levels and declined below initial levels to nearly undetectable levels beginning at 15d following endodormancy-inducing conditions.

Antibody verification and CHIP assays
Antigenicity of the DAM antibody was verified by immunoblot analyses using a GST-DAM fusion protein. The results revealed that DAM antibody correctly recognized the GST-DAM1 and GST-DAM2 protein (S2 Fig). Additionally, only two highly similar genes (or alleles of the same gene) have been found in leafy spurge through genomic and cDNA sequencing that would likely react with the antibody (S2 Table). Several other related genes were also identified that have amino acid sequences with varying degrees of similarity to the sequence that the antibody was designed to recognize (S2 Table). In an unrelated study, only four of these genes were shown to have transcripts that accumulated to relatively high levels in ecodormant buds. Based on these results, the potential for DAM regulation of FT was investigated by ChIP analysis using the DAM-binding antibody and endodormant crown buds when DAM was most highly induced, and FT was concomitantly down-regulated (Fig 3). Five primer pairs from the FT2 promoter region were designed for quantitative PCR following ChIP (Fig 5A) and the precipitated DNA fragments were mainly enriched in regions 1, 2 and 5. In addition, greater quantities of DNA fragments were pulled down with higher doses of antibody ( Fig 5B). However, under the same conditions, addition of greater than 30 μg antibody per sample did not increase the amount of precipitated DNA fragments (data not shown). It is clearly indicated that one or more DAM-like proteins binds the promoter region of FT2 in crown buds during endodormancy. We analyzed the FT2 promoter region and marked the putative conserved transcription factor binding sites. In region 1, an unknown but conserved putative binding site (tttcatgatgggtt) was found. This motif is also conserved in the promoters of leafy spurge FT4 and poplar FT2 (Potri.010G179700 with sequence tttcatgctgggtt). Two other putative CArG boxes were identified in region 2. No additional conserved or known regulatory sequences were identified in the DAM bound region covering the 3´most fragment, but this region was less than 150 bases from the putative TATA box.

DAM and FT may engage different regulatory networks under longphotoperiod conditions
Expression data indicated that DAM and FT transcript abundance appears to follow a similar diurnal rhythm. At least in the case of the DAM transcripts, there appears to be some indication that the expression patterns-particularly the fact that they appear to peak prior to the beginning of the night cycle, are consistent with a circadian response. DAM genes are proposed to be functional homologues to arabidopsis SVP genes [19]. In arabidopsis, SVP is negatively regulated by the circadian clock proteins LHY and CCA1 [10], [28], [29] and protein accumulation of SVP in arabidopsis [28] mirrors transcript abundance (Fig 1) of DAM in leafy spurge. It is unclear if DAM is also regulated by circadian clock associated proteins in perennial plants in a manner similar to SVP of arabidopsis. However, the presence of conserved evening elements in the promoters of leafy spurge DAM genes [19] is consistent with this possibility. Additional experiments with a shift from diurnal photoperiods to constant light or darkness will be required to determine if the response was the result of circadian regulation or not.
In arabidopsis, FT expression levels are influenced by the regulation of CONSTANS (CO) abundance [30]. Under long-photoperiod conditions, the transcript abundance of CO is regulated by the circadian clock [31] and its posttranscriptional regulation is triggered by light [32], [33], [34]. Therefore, in arabidopsis, the activation of FT transcription mainly depends on the light-mediated posttranscriptional regulation of CO [30]. In leafy spurge under long-photoperiods, FT expression levels also appear to follow the light status with peak expression at the end of the photoperiod with no apparent anticipation of a light or dark cycle. Thus, our observations would be consistent with a CO/FT regulatory module under long-photoperiod conditions controlling FT expression in leafy spurge, assuming that the products of these homologs perform similar function in leafy spurge as they do in arabidopsis. Overall, the diurnal expression patterns of DAM and FT in leafy spurge appear to be similar to the diurnal expression patterns or SVP and FT in arabidopsis.

Expression of DAM and FT in leafy spurge are similar to that in other species
Tissue-specific gene expression studies indicated that the DAM1 transcript is preferentially expressed in shoot apices and crown buds, and DAM2 transcript is preferentially expressed in stems and shoot apices, with some expression in leaves (Fig 2). In numerous perennial species, DAM genes are highly expressed in the shoot apices [35] and references therein. Expression of all six tandem-arrayed DAM genes of peach are detectable in vegetative tissues, bud tissues, and with the exception of DAM1, also in floral organs [36]. Similarly, leafy spurge DAM1 and DAM2 transcripts were detected in most vegetative organs (Fig 2). These results suggest that DAM genes have no strict tissue specificity. However, the increased transcript abundance in shoot apices and crown buds and decreased transcript abundance in flowers, leaves, and stems suggest that DAM genes are preferentially expressed in the meristem or forming leaf tissues in leafy spurge as in other species.
Both FT2 and FT4 had greater transcript abundance in flowers and leaves; FT2 also had significant transcript accumulation in stems and detectable transcript levels in shoot apices. FT plays a central role in mediating the onset of flowering, as a mobile signal, which is generally produced in leaves and vascular tissues and transported to the shoot apex [5]. In leafy spurge, the increased abundance of FT in leaves and flowers are consistent with a role for FT in flowering regulation, assuming that these homologs functions similarly in leafy spurge as it does in other species. In Lombardy poplar, PnFT1 and PnFT2 also had strong expression in early-summer leaves and capsules of reproductive trees, while PnFT3/4 had strong expression in stems and winter lateral buds. Further, it is known that the two orthologues of FT genes, FT1 (equivalent to FT3/4 above) and FT2 (equivalent to FT1 and 2 above), from poplar have different expression patterns and likely regulate flowering and vegetative growth differentially [37]. The lack of cold-induced expression for two leafy spurge FT genes is consistent with the expression pattern of the poplar FT2 gene that controls vegetative growth. The combined sequence similarity of leafy spurge FT2 and FT4 with the conserved promoter element of poplar FT2 but not FT1 further suggests that the cloned leafy spurge FT genes most likely are functional orthologues of poplar FT2. However, functional confirmation in leafy spurge or other model perennial systems will be required to verify this hypothesis.

Examination of gene expression evidence for regulation of FT by DAM
The lack of opposite diurnal expression patterns of DAM and FT is inconsistent with a direct role for DAM regulation of FT. Instead, both DAM and FT share common diurnal expression patterns and, thus, there may not be a direct regulatory relationship between the accumulations of their respective transcripts in regards to regulation of diurnal expression. However, in arabidopsis, where a direct regulatory pathway of SVP and FT genes has been established, both genes also appear to be similarly diurnally expressed with highest expression in the evening [10], [28], [29], [38].
The lack of accumulation of either DAM transcripts in leaf tissues following endodormancy-inducing conditions is problematic. Given the hypothesis that DAM regulates FT in leafy spurge the way SVP regulates FT in arabidopsis [21], we expected a strong increase in DAM expression coordinately with repression of FT expression in the leaves. It is unclear if the lack of DAM induction was due to the observed increasing senescence of the leaves after several weeks of endodormancy inducing conditions (data not shown), or if some other factors were responsible for the limited increase in DAM accumulation. However, a slight accumulation of DAM1 transcript in the leaves appears to occur after 7d in leaf tissue following endodormancy induction and this precedes the significant drop in FT expression after 8d. Thus, it is possible that the repression of the FT genes under endodormancy-inducing conditions could be due to DAM1 or DAM2 expression. Likewise, in the shoot tips, FT transcript accumulation did not drop below its initial levels for nearly a week after the observed strong increase in DAM expression. Because we did not observe changes in FT expression prior to observing changes in DAM expression, these observations are consistent with the potential role for DAM negatively regulating FT.
As noted above, DAM is primarily induced and expressed in the buds and meristem (Fig 2). In arabidopsis, SVP is also accumulates in the meristem where it prevents flowering in part by inhibition of the induction of SOC1 by FT [29] and by direct repression of SUPPRESSOR OF CONSTANS 1 (SOC1) [10]. Variation of SOC1 alleles has been associated with dormancy release in peach [39]. A SOC1 homologue in strawberry (Fragaria vesca) was also shown to positively control vegetative growth [40]. SOC1 was down-regulated in poplar following endodormancy induction [41]. Thus, it is also possible that DAM might also regulate endodormancy induction in leafy spurge through mechanisms similar to how SVP regulates flowering in arabidopsis. In support of this hypothesis, SOC1 was down regulated by endodormancyinducing conditions in leafy spurge in two separate microarray experiments [19], [42] and recently in an independent RNAseq experiment (unpublished), albeit not at our fairly strict significance cutoff of p<0.005.
Our results are, however, consistent with DAM having a potential negative regulatory role in FT gene expression in the leaves since the significant decrease in FT expression did not precede the significant increase in DAM1 transcript accumulation. Likewise, previous experiments indicated that FT expression drops significantly during endodormancy and concurrently with an increase in DAM expression [21]. Also, although minimally expressed in the shoot tip (which includes young expanding leaves), FT2 expression became undetectable following the increase in DAM expression. Additionally, other tissues with high DAM expression invariably had low FT expression (Fig 2). Although both DAM and FT transcripts accumulated in leaves under long-photoperiod conditions, the change in accumulation of DAM transcripts during endodormancy-inducing conditions was most evident in shoot apices and crown buds. Thus, although the coordinate expression of DAM and FT are consistent with a role in dormancy regulation, the fact that they are preferentially expressed in different tissues make it unclear if DAM directly regulates FT to induce endodormancy.

Induction of DAM and repression of FT precedes endodormancy induction
Endodormancy was induced in crown buds with the exposure of whole plants to a combination of long-photoperiods with cold evening, night, and morning conditions (Fig 3). The complex mechanism of perceiving changes in temperature and photoperiod in perennial plants has been well discussed [3], [4], [18], [43]. Crown buds became fully endodormant after four to seven weeks of dormancy-inducing treatment, as shown by comparing regrowth from crown bud with treated (endodormant), control (paradormant), and vernalized (ecodormant) plants (Fig 4). Other studies have shown similar levels of leafy spurge growth inhibition in response to low temperatures and short photoperiods [1], [3], [42]. Both leafy spurge DAM1 and DAM2 transcripts showed some accumulation in shoot tips after the first day of endodormancyinducing treatment followed by fluctuating RNA levels for approximate one week, and then a sharp and constant increase in accumulation one week after of treatment. The slope of the accumulation of DAM transcripts in crown buds is consistent with induction prior to the first collected time point at four weeks. The expression of leafy spurge FT genes was coordinately repressed when DAM genes were up-regulated-see above.
The one to two week delay prior to induced accumulation of DAM transcripts and repression of FT intimates a signaling mechanism requiring long term cold (longer than the short cold periods needed to induce many cold-regulated genes in arabidopsis [44]. Because temperature generally fluctuates in fall, a short cold period would not be a reliable signal for endodormancy initiation. In this study, the induction of endodormancy was not evident until somewhat later than the observed increase in DAM, and decrease in FT transcript accumulation. However, the earliest test for endodormancy induction was four weeks, and thus we cannot rule out the immediate establishment of endodormancy with induction of DAM and repression of FT. Thus, although our observations are consistent with these genes having a role in endodormancy induction, it is likely that other upstream and downstream genes or signaling processes may also be involved.

DAM may regulate FT by binding a cryptic CArG box in promoter region
Because DAM and FT expression analysis was suggestive, but not convincing, we investigated the direct binding of DAM to the FT promoter using ChIP assays. Dose-dependent pull-down assays with leafy spurge DAM antibody indicated that leafy spurge DAM (or another very similar protein) interacts with the FT promoter, mainly with the regions 1 and 5 within the promoter of FT2 (Fig 5). Thus, the DAM (or DAM-like) proteins likely directly bind to these regions in vivo, or interacts directly with other transcription factors that bind to these regions. In arabidopsis, SVP negatively regulates FT by binding to the CArG motifs in the promoter region [5], [38], and six CArG motifs were identified from 1.8-kb promoter region of FT and one from the first intron [45]. It was also demonstrated that the vCArG III/IV and vCArG V motifs were efficiently precipitated by SVP-HA antibody in ChIP assays [38]. In this study, regions 1, 2 and 5 of the leafy spurge FT2 promoter have similar locations with variants of CArG III/IV and CArG V motifs in the FT promoter region of arabidopsis. A search of the FT2 promoter region highlighted putative transcription factor binding sites and conserved motifs (S3 Fig). Interestingly, an unknown motif (tttcatgatgggtt) was found in region 1, which could represent an atypical CArG motif. Moreover, this motif is also conserved in similar regions of the leafy spurge FT4 and poplar FT2 promoters. Because CArG box sequences can be variable for SVP protein binding [46], we suggest that this conserved motif may serve as a novel CArG box that is recognized by the leafy spurge DAM protein. However, the region 2 was also enriched in the ChIP assays, and this region contains two CArG boxes, one of which (with the sequence cctattgg) is very similar to a CArG box in the same promoter region of the poplar FT2 gene promoter (with the sequence cctcttgg) and which is identical in the first 6 bases to the CArgIII binding site in the arabidopsis FT gene.
It should be noted though, that only DAM1/2 and three other DAM-like transcript shows high accumulation during ecodormancy (data not shown). Additionally, it has been proposed that DAM and other related proteins likely have additional targets besides FT [22]. We also agree that other targets (such as SOC1) might be viable targets for DAM and that such interactions have a potential role in dormancy responses. Consequently more work is needed to determine not only the additional targets of DAM proteins, but also the complex interactions of other potential regulators of dormancy on expression of FT. Towards this end, we have developed an antibody that recognizes the DAM protein and have data from chromatin immunoprecipitation assays that indicates it can specifically interact with chromatin that includes the conserved CArG box in the FT promoter. However, further studies are needed to confirm the specificity of this antibody and the specificity of the CArG box binding supported by these initial observations.
In conclusion, evidence here and in the literature strongly suggest the DAM/FT regulatory module may be a common mechanism controlling dormancy development in perennial plants.
FT genes, as crucial developmental regulators, have multifaceted roles in plant developmentincluding bud dormancy [16], [37], [47]. FT is regulated by both epigenetic mechanisms and by many regulatory factors or complexes binding to different elements in the promoter or introns of the FT gene [5]. DAM has similarity to other proteins known to negatively regulate FT in other systems. Leafy spurge DAM and FT genes have opposite seasonal expression patterns during para-to endo-dormancy transition in the fall [19]. Here we show that DAM and FT have expression patterns that generally consistent with the hypothesis that DAM negatively regulates FT. Additionally a conserved CArG box exists in the FT promoters of leafy spurge and poplar, and may serve as the binding sites for DAM or other very similar proteins. Further, conservation of the potential DAM binding site in poplar and leafy spurge suggests that the DAM/FT regulatory module may be a common mechanism controlling endodormancy induction in perennial plants. Collectively, these results provide further evidence for DAM having a direct role in negatively regulating FT in leafy spurge.
Supporting Information S1 Table. List of primers. Primers used for qRT-PCR to determine gene expression and chromatin immunoprecipitation result analysis. (DOCX) S2 Table. List of contigs with similarity to predicted DAM1 antibody binding site. List of transcripts assembled from three different expression analyses of leafy spurge including two containing dormant and growing bud samples that were identified as having sequence similarity (E values < 10E-5) with DAM1 in a BlastX search of the transcriptome database. Highlighted contigs are from genes that have good levels of expression (> 100 transcripts per million) in ecodormancy. showing coomassie-stained gel (left) containing GST-tagged and GST-column purified proteins expressed in E. coli. Gel was blotted and hybridized with 100 μg of anti-DAM1 diluted into 10 ml of TBST. The DAM1 antibody specifically reacted with both GST-tagged DAM1 (the 30kD band) and DAM2 (the 50 kD band) which also contains the target amino acid sequence. Note that that co-purified proteins that eluted with the GST-tagged DAM proteins did not hybridize to the DAM1 antibody, thus indicating some level of specificity. The nature of the antigenic 30 kD band that eluted with the GST-tagged DAM2 protein is unknown, but is likely to be a breakdown product of the DAM2 protein. The level of specificity of the antibody in vivo under nondenaturing conditions is unclear, but given the fact that likely DNA targets of DAM1 can be specifically precipitated with this antibody, it seems likely that it is reactive in vivo. (DOCX) S3 Fig. FT2 promoter sequence and likely transcription factor binding sites. Above shows FT2 promoter region and the putative conserved transcription factor binding sites are marked. The pull down DNA fragments mainly enriched in P1, P2 and P5 amplification regions. P1 and P5 amplicons are noted in bold regions, and the P2 region is underlined. Colored boxes indicate possible transcription factor binding sites. (DOCX)