Migration of Myeloid Cells during Inflammation Is Differentially Regulated by the Cell Surface Receptors Slamf1 and Slamf8

Previous studies have demonstrated that the cell surface receptor Slamf1 (CD150) is requisite for optimal NADPH-oxidase (Nox2) dependent reactive oxygen species (ROS) production by phagocytes in response to Gram- bacteria. By contrast, Slamf8 (CD353) is a negative regulator of ROS in response to Gram+ and Gram- bacteria. Employing in vivo migration after skin sensitization, induction of peritonitis, and repopulation of the small intestine demonstrates that in vivo migration of Slamf1-/- dendritic cells and macrophages is reduced, as compared to wt mice. By contrast, in vivo migration of Slamf8-/- dendritic cells, macrophages and neutrophils is accelerated. These opposing effects of Slamf1 and Slamf8 are cell-intrinsic as judged by in vitro migration in transwell chambers in response to CCL19, CCL21 or CSF-1. Importantly, inhibiting ROS production of Slamf8-/- macrophages by diphenyleneiodonium chloride blocks this in vitro migration. We conclude that Slamf1 and Slamf8 govern ROS–dependent innate immune responses of myeloid cells, thus modulating migration of these cells during inflammation in an opposing manner.

The cell surface glycoprotein Slamf8, as well as human SLAMF8 (BLAME), is expressed by a variety of myeloid cells such as neutrophils, macrophages, monocytes and DCs upon encountering Gram-or Gram+ bacteria, LPS or IFN-γ [9,10]. In contrast to Slamf1, Slamf8 negatively regulates ROS production by inhibiting Nox2 activity and conversely Nox2 activity of Slamf8 -/macrophages is increased upon exposure to bacteria. One explanation could be that Slamf8 regulate protein kinase C activity, which is increased in Slamf8 -/macrophages causing an enhanced phosphorylation of p40phox, in turn leading to greater Nox2 activity [10].
The classic paradigm that production of ROS by phagocytes is instrumental in their bactericidal activity is complemented by more recent studies that have established a role in the activation of many signaling pathways and the regulation of antigen cross-presentation [11,12]. Perhaps more importantly, ROS also affects cell adhesion and migration [13,14]. Recently two other groups independently reported that Nox2 regulates CSF-1 mediated macrophage and microglial chemotaxis [15,16]. In support of this notion are our observations that infiltration of Slamf1 -/macrophages and monocytes to the sites of inflammation is markedly reduced in two models of enterocolitis and in peritonitis [17].
The present study evaluates the respective roles of Slamf1 and Slamf8 in migration of dendritic cells (DCs), macrophages, and neutrophils by employing in vivo and in vitro assays. Not only does a correlation exist between the level of ROS production and altered migration in a cell-intrinsic fashion, the use of a Nox2 inhibitor prevents in vitro migration. As Slamf8 is a homophilic cell surface receptor, which is expressed on endothelial cells, we postulate that Slamf8 may control migration by adhesion to the lymphatic capillaries. Similarly, expression of Slamf8 by fibroblastic reticular cells (FRC) [18] suggests that this receptor may play a role in migration of DCs along FRC-containing conduits inside the lymph nodes.

Materials and Methods Mice
Slamf1 -/and Slamf8 -/-Balb/c mice were described previously [5,10]. Age and sex matched wt Balb/c mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). All animals were maintained under specific pathogen-free conditions at the Center for Life Science animal facility of Beth Israel Deaconess Medical Center (BIDMC) and were used at 8-12 weeks of age. The experiments were performed according to the guidelines of the Institutional Animal Care and Use Committee (IACUC) at BIDMC. or human IgG-Fc (Jackson ImmunoResearch Laboratories) was intraperitoneally injected 3 hours before 200μL FITC application. Single-cell suspensions were stained and analyzed by flow cytometry.

In vivo Thioglycollate broth-induced peritonitis
Wt, Slamf1 -/and Slamf8 -/mice were intraperitoneally injected with 2mL of sterile 4% thioglycollate broth. Four hours or four days later, mice were euthanized. The cells from the peritoneal cavity were washed out with 10mL of RPMI medium and stained for FACS.
In vivo Anti-CD3 induced myeloid cell migration model 20μg of purified αCD3ε (145-2C11) (Biolegend, San Diego, CA) or Mouse IgG2b (Biolegend, San Diego, CA) were intraperitoneally injected. Three or five days later the mice were euthanized. Lamina propria cells were isolated and analyzed by flow cytometry [20].

Intracellular ROS detection
Intracellular ROS was quantified by using CM-H 2 DCFDA (Life Technologies, C-6827) based on the manufacturer's protocol. CM-H 2 DCFDA was added to RPMI 1640 medium at a final concentration of 10μM. Thioglycollate-elicited macrophages were incubated with CM-H 2 DCFDA at 37°C for 1 hour. After incubation, the macrophages were incubated with heat inactivated E.coli F18 bacteria (multiplicity of infection [MOI] 100). Fluorescence was measured every 5 minutes by flow cytometry for 2 hours.

In vitro Chemotaxis assay
Ear skin was collected and digested for 2 hours with DNase (15μl from 10 mg/ml stock, Sigma) and 500μL Liberase (Roche) in RPMI 1640 at 37°C in a shaking incubator. After filtration through a 70μm cell strainer, single cell suspensions were obtained. CD11c + cells were further isolated using CD11c MicroBeads (Miltenyi Biotec) in a positive selection column. Chemotaxis of skin DCs was measured using a polycarbonate filter with 5μm pores in 96-well transwell chambers (Neuro Probe, Gaithersburg MD). 30μL chemotaxis media was added to the lower chamber, and 2.5 × 10 4 DCs were added to the upper chamber and incubated for 8 hour at 37°C with 5% CO 2 .
In vitro transwell (5μm pore) migration analysis for macrophages was performed using a 96-well migration chamber (NeuroProbe, Gaithersburg MD). The lower wells contained recombinant murine CSF-1 (30ng/mL), and 5.0 × 10 4 Thio-macrophages were added to the upper well to migrate for 150 minutes in a humidified chamber (5% CO 2 , 37°C). In some experiments diphenyleneiodonium chloride (DPI) (5μM) was added to cell suspension 15 minutes prior to migration. Migrating cells were counted according to the manufacturer's protocol by flow cytometry, using counting beads.

Generation of mouse Slamf8-Fc and Slamf1-Fc recombinant soluble protein
The murine Slamf8-Fc and Slamf1-Fc fusion protein containing the Fc region of human IgG1 was obtained by inserting the sequence corresponding to the extracellular domain (including the signal peptides and two extracellular domains) of Slamf8 or Slamf1 into the mammalian expression vector pcDNA4/myc-HisC (Invitrogen). The construct expressing Slamf8-Fc was transfected in NS-1 myeloma cells and the construct expressing Slamf1-Fc was transfected in 293F cells. Stable Slamf8-Fc or Slamf1-Fc expressing cells were selected with zeocin and expanded for large-scale culture. The supernatants containing the fusion proteins were purified with a protein G agarose bead column from the supernatant.

Slamf8-Fc fusion protein binding assay
Mouse Slamf8 cDNA were cloned into pcDNA4.0 Myc/his vector (Life Technology, Carlsbad, CA, USA). HEK293 cells were transfected with mouse Slamf1 or Slamf8 cDNAs using FuGENE 6 Transfection Reagent (Roche). 24 hours after transfection, 10μg of Fc fusion proteins Slamf1-Fc or Slamf8-Fc were incubated with 1×10 6 transfected HEK293 cells on ice for 60 minutes, followed by incubation with biotin-conjugated goat anti-human IgG Fc (Life Technologies) and then streptavidin-PE. Binding was assessed by flow cytometry.

Preparation of Mouse Heart Endothelial Cells
Mouse heart endothelial cells (MHEC) were prepared from the heart of newborn mice (7-9 days old) as described [21]. 100ng/ml of TNF-α was used to stimulate endothelial cells for 24 hours, and the cell RNA was isolated.

Statistical analysis
The Prism 5.0 software (GraphPad, San Diego, CA, USA) was used to analyze the data. Results are reported as mean ± SEM. All the statistical comparisons were performed using the 2-tailed Student's t test. Values of p < 0.05 were considered to be statistically significant.

Ethics statement
The murine protocols used in this study were approved by the BIDMC IACUC (Protocol 020-2012), which is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC). All efforts were made to minimize animal suffering, including the use of isoflurane when appropriate, i.e., in the skin sensitization assay. Euthanasia was performed by CO 2 in an IACUC-approved setup. The use of PBMCs was approved by the Institutional Review Board of Beth Israel Deaconess Medical Center.

Slamf8 negatively regulates in vivo migration of DCs, whilst Slamf1 is a positive regulator
Innate immune cell trafficking is a critical process in triggering specific immune responses. For instance, inflammation of the skin induced by the hapten FITC leads to activation of skin dendritic cells (DCs) and their subsequent migration to the draining lymph nodes, which is primarily mediated by CC chemokine receptor 7 (CCR7) signaling [22]. A skin contact sensitization model was employed to assess whether migration of Slamf8 -/-, Slamf1 -/and wt DCs differed. To this end, the dorsal skin of mice was shaved and painted with 4 mg FITC overnight and cells from the draining lymph nodes were isolated. Migratory DCs were quantitated by flow cytometry by gating for CD11c + MHC hi FITC-positive cells (Fig. 1A) [23]. Compared to wt mice, the percentage and total number of FITC + migratory DCs were significantly higher in Slamf8 -/mice ( Fig. 1B and 1C). As a difference in the number of dermal DCs may be responsible for the increased number of migrating DCs, the numbers of DCs in the skin of wt and Slamf8 -/mice were compared. The number of CD11c + cells in the skin of the ear were comparable in wt and Slamf8 -/mice (S1A Fig.), showing that there is no numerical difference in the dermal DC population between wt and Slamf8 -/mice [24]. By contrast, fewer FITC +migratory DCs were detected in the draining lymph nodes of Slamf1 -/mice ( Fig. 1D), which was consistent with our previous finding that infiltration of Slamf1 -/monocyte-derived mononuclear phagocytes into the inflamed lamina propria of colitic mice is impaired [17]. As there was no obvious difference in expression of CCR7 on the surface of skin DCs before or after FITC painting of wt and Slamf8 -/mice (S1B and S1C Fig.), the altered DC migration might be due to a mechanism in addition to CCR7 signaling-the major contributor of DC migration to lymph nodes. Taken together, the outcomes of these studies indicate that the cell surface receptors Slamf1 and Slamf8 control in vivo migration of DCs in an opposite manner.

Slamf8 negatively regulates in vivo migration of macrophages to the inflamed peritoneal cavity
Intraperitoneal injection [i.p.] of thioglycollate broth induces peritonitis in mice resulting in infiltration of different inflammatory cells in a time-dependent fashion [25]. Whereas neutrophils are the major infiltrating cells in the peritoneal cavity after 4 hours, macrophages begin to appear after 24 hours and become the major constituents after 48 hours. As shown in Fig. 2A, the total number of cells in the peritoneal cavity of Slamf1 -/-Balb/c mice was lower than the number of cells in wt Balb/c mice four days after induction of peritonitis. However, the number of cells in the peritoneal cavity of Slamf8 -/-Balb/c mice was increased as compared to wt mice ( Fig. 2A). Consistent with the latter observation, four days after the injecting thioglycollate the number of CD11b + F4/80 + macrophages in the peritoneum of Slamf8 -/mice was higher than in the cavity of wt Balb/c mice (Fig. 2B). The number of macrophages in the peritoneal cavity  of Slamf1 -/-Balb/c mice was lower than in wt Balb/c mice (Fig. 2B), confirming our previous observation assessing macrophages in Slamf1 -/-C57BL/6J mice [17]. We conclude that Slamf8 -/macrophages display a stronger migration response to the induction of peritonitis. This supports the concept that Slamf8 plays an inhibitory role in migration of both macrophages and dendritic cells.
Accelerated migration of macrophages and monocytes in the villi of the small intestine of Slamf8 -/mice To confirm the notion of an accelerated in vivo migration of Slamf8 -/myeloid derived phagocytes we used a novel method by which repopulation of CX 3 CR1 + phagocytes in the villi of the small intestine is evaluated. Administration of αCD3 mAb to C57BL/6J mice activates cytotoxic CD8 T cells in the small intestine, which results in a transient depletion of CX 3 CR1 + phagocytes. Migratory monocytes then repopulate the villi of the small intestine [26]. As shown in Fig. 3A and 3B, three days after administering αCD3 to Balb/c mice a significant depletion of the number and percentage of CD11b + CD11c + CD45.2 + CD103mononuclear phagocytes in the small intestine was observed. The majority of these cells are CX 3 CR1 + macrophages of monocytic origin that reside in the small intestine [27,28]. While Slamf8 -/mice appear to have a slightly reduced percentage of intestinal macrophages in homeostatic conditions, their repopulation is significantly faster than that of wt mice. Three and five days post αCD3 injection, the CD11b + CD11c + CD45.2 + CD103cells begin to re-appear, more rapidly in Slamf8 -/than in Slamf8 +/+ mice. Slamf8 -/mice show no statistical difference in the percentage between day 0 and day 5, but Slamf8 +/+ mice have a lower percentage of these cells at day 5 (Fig. 3A). Surprisingly, a steep increase in CD11b + Ly6C + cells was observed in Slamf8 -/mice three days after the last αCD3 injection compared to wt mice ( Fig. 3C and 3D). This population, which was reduced in size at 5 days post injection, is known to represent newly arrived monocytes in the small intestine, which quickly transition to mature macrophages [29]. The results indicate that Slamf8 -/mononuclear phagocyte precursors infiltrate the lamina propria at a faster rate than their wt counterparts supporting the notion that Slamf8 negatively regulates the migratory capacity of myeloid cells to immunologically active sites.
Slamf8 negatively regulates Nox2 activity and in vivo migration of neutrophils Neutrophils, which migrate from the blood to a site of inflammation, represent the first line of defense against bacteria. Because we found a very high Nox2 activity in Slamf8 -/macrophages and DCs (S2 Fig.) [10], we tested the ROS production by Slamf8 -/neutrophils. As judged by a lucigenin-based assay, Slamf8 -/neutrophils produce indeed significantly more ROS than wt Balb/c neutrophils in response in response to E. coli or to PMA (Fig. 4A).
We next evaluated the role of Slamf8 in neutrophil migration into the peritoneal cavity of wt and Slamf8 -/mice 4 hours after i.p. injection of thioglycollate. Consistent with the macrophage data obtained 4 days after administering thioglycollate (Fig. 2), the total number of cells obtained from the peritoneal cavity of Slamf8 -/mice was greater than the number of cells obtained from wt mice (Fig. 4B). Importantly, the number of CD11b + Ly6G + peritoneal neutrophils in Slamf8 -/mice was 2-fold higher that of wt mice (Fig. 4C-4E).
We also evaluated migration of neutrophils in the villi of the small intestine on day 3 and 5 after administering αCD3 to Slamf8 -/and wt mice (Fig. 4F-4G). Whilst the percentage of neutrophils in the small intestine comprised~14% of the CD45.2 + cells in Slamf8 -/mice on day 3 post injection, the percentage of neutrophils was~5% on day 5. By contrast, on day 3 after administering αCD3 neutrophils comprised~1% of the CD45.2 + cells in the lamina propria of wt mice, whereas no neutrophils were detected on day 5. In conclusion, Slamf8 negatively affects migration of neutrophils in two in vivo models consistent with the altered migration of DCs and monocyte-derived macrophages in Slamf8 -/mice.

Altered in vitro migration of Slamf8-and Slamf1-deficient DCs
To test the hypothesis that cell intrinsic functions of Slamf1 and Slamf8 give rise to an altered migratory capacity, chemotaxis was examined in a transwell migration assay were. We focused on CCR7 and its two major ligands, CCL21 and CCL19, which play an important role in facilitating migration of DCs in the lymphatic system and within lymph nodes [30]. DCs that were obtained by CD11c positive selection from the skin of the ear were allowed to migrate through 5μm pores in a transwell system toward CCL21 [0-400nM]. Slamf8 -/migrated much more efficiently toward CCL21 than wt Balb/c DCs (Fig. 5A). In vitro trafficking of skin-derived Slamf8 -/-DCs in response to CCL19 was similarly increased (S3 Fig.). In contrast to Slamf8 -/cells, migration of Slamf1 -/skin-derived DCs toward CCL21 was markedly reduced (Fig. 5B). These differences in in vitro migration indicate that this process is DC-intrinsic. As the surface expression of CCR7 is similar between Slamf8 -/and wt DCs the differences between wt and deficient DCs could not be due to altered expression of CCR7 (S1B and S1C Fig.).
A role for Nox2-dependent production of ROS in migration of bone marrow derived macrophages in response to the chemo-attractant CSF-1 was suggested by experiments in gp91 -/mice, which lack an active Nox2 complex [15,31]. To test the hypothesis that cell intrinsic functions of Slamf1 and Slamf8 affect cell migration in response to CSF-1 in a ROS-mediated fashion an in vitro transwell system was used in the absence and presence of the Nox2 inhibitor diphenyleneiodonium chloride (DPI), which effectively inhibits human neutrophil migration [14]. First, we determined that 5μM of DPI inhibited PMA induced Nox2 activity of peritoneal macrophages without affecting cell viability ( Fig. 5C and S4 Fig.). In response to CSF-1, in vitro migration of Slamf1 -/peritoneal macrophages was impaired, while an enhanced migration of Slamf8 -/macrophages was observed (Fig. 5D). This observation is dependent on ROS as incubation with DPI 15 minutes before the cells were allowed to migrate toward CSF-1 dramatically reduced the number of migrating cells. Thus, cell intrinsic migration towards CSF-1 is altered in Slamf1 -/and Slamf8 -/macrophages. Additionally, this cannot be due to altered expression of the CSF-1 receptor CSF-1R (CD115), as shown in S5 Fig. Taken together, these data suggest the signaling of two key cell surface receptors CCR7 and CSF-1R is altered in the Slamf1-and Slamf8-deficient cells in a ROS-dependent fashion.

The self-ligand Slamf8 affects the in vivo migration of DCs
The notion that majority of human and mouse Slamf adhesion molecules are homophilic prompted us to evaluate whether Slamf8 would be a self-ligand receptor. To this end, a soluble fusion protein comprised of the mouse Slamf8 ectodomain and the human IgG1 Fc domain (Slamf8-Fc) was generated. Next, 293 cells transiently transfected with Slamf8 or Slamf1 were incubated with the Slamf8-Fc protein and stained with a fluorescent antibody directed against the Fc portion of human IgG [32]. As predicted, Slamf8-Fc associated with Slamf8 transfected 293 cells, but not with Slamf1 transfectants. In the reciprocal experiment Slamf1-Fc associated with Slamf1 transfected cells but did not bind to Slamf8 transfected 293 cells (Fig. 6A-6C). Thus, Slamf8 is a homophilic receptor, which does not ligate in trans with Slamf1.
Next, the effect of Slamf8-Fc in the skin contact sensitization model was evaluated. To this end wt mice were injected with the Slamf8-Fc fusion protein 3 hours prior to painting 2 mg FITC onto the shaved skin. Mice that had received the Slamf8-Fc fusion protein both the percentage and the number of migratory DCs were significantly elevated compared to the mice injected with human IgG1 (Fig. 6D-6F). This suggests that Slamf8-Fc interrupted the homophilic Slamf8 interactions that suppress DC migration (Fig. 1).

Discussion
Three distinct in vivo models, i.e. skin sensitization by painting with the hapten FITC, thioglycollate induced peritonitis and migration in the lamina propria of the small intestine, were employed to demonstrate that migration of Slamf8-deficient DCs, macrophages and neutrophils is increased. This indicates that Slamf8 negatively regulates this process in wt mice. In vitro transwell experiments showed an enhanced migration of Slamf8 -/macrophages in response to CSF-1 and dendritic cells in response to the chemokines CCL19 and CCL21, which bind to the CCR7 receptor [30]. The increased migration of Slamf8 -/macrophages and DCs is cell intrinsic and macrophage migration is inhibited by the Nox2 inhibitor DPI. We conclude that Nox2- Slamf8 and Slamf1 Regulate Myeloid Cells Migration dependent ROS production, which raises intracellular and extracellular levels of H 2 O 2 causes accelerated migration of Slamf8 -/phagocytes. To our knowledge, Slamf8-deficient mice are one of two mutant mice with an inducible increased ROS production, the other being the recently discovered Negative Regulator of ROS (NRROS) [33], which was studied in the context Slamf8 and Slamf1 Regulate Myeloid Cells Migration of autoimmune disease. In wt cells Slamf8 is therefore a negative regulator of signal transduction events in myeloid cells, which affect their migration by interacting with its self-ligand on endothelial cells or fibroblastic reticular cells. By contrast, Slamf1 appears to enhance migration of phagocytes by stimulating Nox2-dependent events. Hence, myeloid cell migration is consistently impaired in Slamf1 -/mice, which generate low levels of ROS in response to several bacteria, LPS and other inflammation related biologicals [6,17].
The classical phagocytic NADPH oxidase (Nox2) produces reactive oxygen upon assembly of the membrane bound catalytic gp91 phox and p22 phox with at least four cytosolic subunits p40 phox , p47 phox , p67 phox , Rac1/2 [11]. Nox2 complex assembly is a tightly regulated process that involves phosphorylation of its cytosolic subunits or binding of p40 phox to the lipid phosphatidyl-3'-phosphate (PI 3 P), which is phosphorylated by the Class III phosphatidyl inositol kinase Vps34 [34]. The latter enzyme can be recruited by the intracellular tail of Slamf1 and in the absence of Slamf1 macrophages, dendritic cells, and neutrophils produce reduced amounts of ROS and consequently H 2 O 2 [6,7]. ROS in phagocytes, which plays a role in killing of some bacteria, is also involved in many aspects of innate immune responses, e.g. NF-κB signaling through TRAF6 recruitment [35]. Perhaps the best-known signaling effects of ROS are in oxidation of specific Cys residues of the protein tyrosine phosphatases SHP-1 and SHP-2, which impair their enzymatic function thus allowing for enhanced phosphorylation by tyrosine kinases [36,37]. Another example of these phosphatases is the lipid phosphatase PTEN, which impact directional mobility [38]. Effects on cytoskeletal functions is exemplified by β-actin Cys374 that can be S-glutathionylated by ROS thereby promoting cell spreading, which is a necessary step in adequate migration [39]. DC migration was shown to be affected through the function of oxidative stress response kinase 1 (OSR-1) [40]. Direct evidence of the involvement of Nox2 in cell migration and chemotaxis comes from studies that used mice with genetic ablations of Nox2 components. For instance, Nox2 knock out (gp91 phox-/-) bone marrow macrophages completely lost their chemotactic response toward a CSF-1 gradient, which is likely due to a reduction of ERK1/2 phosphorylation leading to defected cell migration [15]. In the murine macrophage cell line RAW264.7 Nox2 is required for LPS induced matrix metalloproteinase MMP-9 expression, which eventually influences cell migration [41]. In sum, ROS signaling has implications on a variety processes including migration, adhesion and chemotaxis.
Using a small-molecule screen for drugs capable of inhibiting neutrophil chemotaxis, Hattori and colleagues found that the Nox2 inhibitor diphenyleneiodonium chloride (DPI), can effectively inhibit human neutrophil directionality during migration. Furthermore, they found that both gp91 phox-/murine neutrophil and neutrophil from Chronic granulomatous disease (CGD) patients lost their directional migration, establishing the notion that Nox2 activity influences cell migration [14].
Our previous study shows that expression of murine Slamf8 is not only significantly induced in macrophages by bacteria and bacterial components, e.g. E. coli and LPS, but more dramatically by IFN-γ [10]. As shown in S6A-D Fig., IFN-γ also induces Slamf8 transcripts in bone marrow derived DCs, peritoneal neutrophils, human monocytes and macrophages and protein expression in peritoneal macrophages. Perhaps more importantly, Slamf8 expression can be induced by TNF-α treatment of mouse heart endothelial cells (S6E Fig.), consistent with the notion that the homophilic interaction might play a role in cell migration. That concept is also supported by the observation that Slamf8 is expressed in skin and mesenteric lymph nodes by fibroblastic reticular cells (FRC) [18,42], which function as a scaffold for the support and migration of hematopoietic cells [43].
Based upon this study and published work employing Slamf1 as well as Slamf8 deficient mice and mutant macrophage cell lines [5-7, 10, 17], we propose a model in which Slamf1 and Slamf8 help shape innate immune responses (Fig. 7). This includes differential expression and induction of Nox2 activity in response to innate stimuli [6,10]. Furthermore, the model graphically represents how migration of various myeloid cells is dependent upon the two receptors. Initial inflammatory queues may trigger Slamf1-meditated Nox2 activation resulting in a spike in ROS, which is rapidly converted into hydrogen peroxide that contributes to the migratory activity of Slamf1-expressing cells. Subsequent inflammatory mediators (IFN-γ) induce Slamf8 expression, which suppresses the production of ROS and hence reduces additional migratory infiltration.
Taken together, the outcomes of our studies provide the first evidence that Slamf8 is a novel homophilic receptor, and that Slamf1 and Slamf8 oppositely regulate macrophage migration in a Nox2 dependent manner. The similarity of the in vivo migration and in vitro ROS production phenotypes suggest that Nox2 has a similar involvement in the migration of DCs and neutrophils as macrophages. These findings point out that Slamf1 and Slamf8 are potential therapeutic targets to modulate DC, macrophage and neutrophils migration and function, thus Slamf1 and Slamf8 based therapeutic strategies can be pursued to regulate cellular immune response. As Slamf8 -/mice show an enhanced migration of antigen presenting cells and Slamf8-Fc approximates the Slamf8 -/phenotype, Slamf8 may be of interest for potential therapeutic applications in vaccine development. In migratory phagocytes, the early actor Slamf1 enhances ROSmediated migration signals; subsequently Slamf8 reduces ROS-mediated migration signals. Inflammatory signals enhance the activity of Slamf1, which induces Nox-2 mediated ROS production. Inflammatory mediators, such as IFN-γ subsequently increase Slamf8 expression and function. This leads to the suppression of ROS production resulting in the reduction in infiltration of phagocytes. Thus, Slamf1 and Slamf8 together, balance the extent of infiltration of inflammatory cells.