Functional Characterization of a Nudix Hydrolase AtNUDX8 upon Pathogen Attack Indicates a Positive Role in Plant Immune Responses

Nudix hydrolases comprise a large gene family of twenty nine members in Arabidopsis, each containing a conserved motif capable of hydrolyzing specific substrates like ADP-glucose and NADH. Until now only two members of this family, AtNUDX6 and AtNUDX7, have been shown to be involved in plant immunity. RPP4 is a resistance gene from a multigene family that confers resistance to downy mildew. A time course expression profiling after Hyaloperonospora arabidopsidis inoculation in both wild-type (WT) and the rpp4 mutant was carried out to identify differentially expressed genes in RPP4-mediated resistance. AtNUDX8 was one of several differentially expressed, downregulated genes identified. A T-DNA knockout mutant (KO-nudx8) was obtained from a Salk T-DNA insertion collection, which exhibited abolished AtNUDX8 expression. The KO-nudx8 mutant was infected separately from the oomycete pathogen Hpa and the bacterial pathogen Pseudomonas syringae pv. maculicola ES4326. The mutant displayed a significantly enhanced disease susceptibility to both pathogens when compared with the WT control. We observed a small, stunted phenotype for KO-nudx8 mutant plants when grown over a 12/12 hour photoperiod but not over a 16/8 hour photoperiod. AtNUDX8 expression peaked at 8 hours after the lights were turned on and this expression was significantly repressed four-fold by salicylic acid (SA). The expression of three pathogen-responsive thioredoxins (TRX-h2, TRX-h3 and TRX-h5) were downregulated at specific time points in the KO-nudx8 mutant when compared with the WT. Furthermore, KO-nudx8 plants like the npr1 mutant, displayed SA hypersensitivity. Expression of a key SA biosynthetic gene ICS1 was repressed at specific time points in the KO-nudx8 mutant suggesting that AtNUDX8 is involved in SA signaling in plants. Similarly, NPR1 and PR1 transcript levels were also downregulated at specific time points in the KO-nudx8 mutant. This study shows that AtNUDX8 is involved in plant immunity as a positive regulator of defense in Arabidopsis.


Introduction
Plants are sessile organisms that evolved remarkable signaling pathways in order to cope with several abiotic and biotic stresses such as pathogen attack. In one branch of the plant immune system there are nucleotide binding leucine-rich repeat (NB-LRR) proteins in the cell that recognize a plethora of pathogen effectors from several kingdoms and activate a cascade of signaling pathways ultimately leading to effector triggered immunity (ETI) [1,2]. In Arabidopsis Columbia ecotype (Col-0), the resistance (R) gene RPP4 confers resistance to the oomycete pathogen Hyaloperonospora arabidopsidis (Hpa) isolates Emwa1 and Emoy2 (downy mildew), which involves multiple defense signaling components, including the NPR1 protein among others [3].
Some plant immune responses are associated with NPR1 protein conformational changes induced by redox levels [4]. NPR1 is a well-known master regulator of pathogenesis related (PR) gene expression and salicylic acid (SA) signaling [5,6]. NPR1 protein resides in the cytoplasm as an oligomer maintained by disulphide bonds that are sensitive to redox changes [4]. Reduction of disulphide bonds cause NPR1 monomer migration to the nucleus and activation of PR gene expression. NPR1 also works upstream of SA suppressing expression of ICS1 and inhibiting SA biosynthesis in a negative feedback loop [7].
Thioredoxins (TRXs) are small cytosolic proteins that act as disulphide reductase proteins [8,9]. In Arabidopsis, there are eight cytosolic types of TRXs, three of which have been related to pathogen attack: TRX-h2, TRX-h3 and TRX-h5 [4]. Of these, TRX-h3 is the only one constitutively expressed. Reduction of the NPR1 oligomer-to-monomer reaction is catalyzed by cytosolic TRXs by the reduction of their intermolecular disulphide bonds. Incubation of NPR1-GFP protein extracts with recombinant TRX-h5 protein can increase the amount of NPR1-GFP monomer [4].
The Nudix (nucleoside diphosphates linked to some moiety X) gene family comprises 29 homologs in Arabidopsis and is well conserved across several species and all domains of life (Eukaryotes, Prokaryotes and Archaea) [10]. Its members contain a conserved Nudix box motif ''GX5EX7REVXEEXGU'' that catalyzes the hydrolytic breakdown of nucleoside diphosphates linked to other moieties by cleavage of chemical bonds. Nudix hydrolases have been shown to catalyze the hydrolysis of nucleoside diphosphates such as nucleotide sugars (ADP-glucose) [11,12,13] and pyridine nucleotides such as NADH, NADPH and 8-oxo-GTP [13,14,15]. Nucleoside diphosphates are key metabolic intermediates and signaling molecules that are often toxic to the cell. It has been proposed that Nudix hydrolases may have a role as house cleaning enzymes by getting rid of toxic, excessive nucleoside diphosphate and hence maintaining normal cellular homeostasis [12,16,17].
Previous phylogenetic analysis of the Nudix gene family in Arabidopsis has further divided these into four subfamilies. AtNUDX8 appears in the fibroblast growth factor type Nudix enzyme (FGFTNE) subfamily in a monophyletic clade. All members in this clade have hydrolase activity towards ADP-ribose and NADH, which is important for defense responses in plants [18]. Most Nudix family members are mainly present in the cytosol (AtNUDX1 to 11 and 25) but there are also organelle-type Nudix hydrolases which localize to the chloroplast and mitochondrion (AtNUDX14, 15, 19, 23, 26 and 27) in Arabidopsis [12]. Enzyme activity has been tested in all Nudix hydrolases (AtNUDX1-AtNUDX27) in Arabidopsis. Only four genes (AtNUDX2, AtNUDX6, AtNUDX7 and AtNUDX10) showed pyrophosphohydrolase activity towards both ADP-rib and NADH in vitro [11]. The involvement of reactive oxygen species (ROS) such as H 2 O 2 in plant defense against bacterial and fungal pathogens was previously demonstrated [19]. Intensive ROS production from NADH pools during oxidative burst might be required by several defense responses such as lignin formation, antimicrobial action and hypersensitive response during systemic acquired resistance [19]. Nudix enzymes may also play a significant regulatory role in photosynthesis in the reduction of O 2 to O 2 + , which is accompanied by H 2 O 2 and OH production [17]. Indeed, mutations in house cleaning enzymes and oxidation protective enzymes could generate an imbalance in cellular homeostasis affecting several outputs in plant signaling such as pathogen defense and hormone signaling [20].
Other studies have shown that both AtNUDX6 and AtNUDX7 genes are involved in plant defense. AtNUDX6 is directly involved in the plant immune response as a positive regulator of NPR1-mediated defense [21]. KO-nudx6 plants showed suppressed levels of several SA-induced, NPR1-dependent genes and also TRX-h5 involved in SA-induced NPR1 activation. KO-nudx6 mutant also displays increased NADH levels [21]. Additionally AtNUDX6 was shown to be involved in SA signaling and its transcript levels induced by SA [21]. AtNUDX7 on the other hand is involved in plant immunity as a negative regulator of defense response. A previously studied T-DNA knockout line Atnudt7-1 exhibited an array of pleiotropic effects such as increased resistance to bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 (avrRpt2) [22] and oomycete pathogen Hyaloperonospora arabidopsidis [23], microscopic cell death and constitutive expression of PR genes [14]. Atnudt7-1 and Atnudt7-2 knockout lines displayed a small stunted phenotype influenced by edaphic factors and high NADH and ADPribose levels [14]. AtNUDX7 transcript levels are SA insensitive unlike its SAinduced homolog AtNUDX6 but its transcript was shown to be induced upon Pst infection [22].
In this study, we investigated the functional role of the AtNUDX8 gene (AT5G47240), a previously uncharacterized member of the Nudix hydrolase family, in defense response upon pathogen attack in Arabidopsis. In order to perform this study, we first identified an Arabidopsis T-DNA insertion knockout mutant for AtNUDX8 (KO-nudx8) and studied disease phenotypes in response to pathogen infection and transcriptional changes observed in pathogen responsive TRXs and NPR1-dependent genes by quantitative real-time PCR. The results obtained in this study indicate that the AtNUDX8 gene is a novel element in plant immune responses against pathogen attack.

Plant Material
Seeds of WT (Col-0) and KO-nudx8 mutant from Salk T-DNA insertion collection (SALK_092325.55.50.x) were sown in pots containing Metro-Mix 360 soil for 3-4 weeks in a growth chamber. Plants were maintained at 22˚C constant temperature and 50% relative humidity. Plants were grown in long day light conditions (16/8 hours of light/dark) for most experiments and a 12/12 hour photoperiod for the small stunted phenotype. For time course, quantitative realtime PCR (qRT-PCR) analysis, three to four leaves from at least three individual plants were collected from the time the lights were turned on in the growth chambers (Zeitgeber time, 0 hour) and five time points were collected (0, 4, 8, 16 and 24 hours). The samples were immediately frozen in liquid nitrogen and stored at 280˚C for RNA extraction.

Molecular analysis of insertion lines
We used a PCR approach to confirm the T-DNA insertion in the AtNUDX8 (AT5G47240) gene using T-DNA left border LBb1 primer (59-GCGTGGACCGCTTGCTGCAACT-39) and gene specific primers LP (59-GCAATATCTTCGAGCAGCAAC-39) and RP (59-TGTTACATTTACCTTTGCGGC-39). Positive, homozygous T-DNA insertion lines for AtNUDX8 were allowed to grow for seed collection.

Pseudomonas infection assay
Whole leaves of three-week-old plants were infiltrated with a bacterial suspension (OD 600 50.0002) of Pseudomonas syringae pv. maculicola (Psm) ES4326 in 10 mM of MgSO 4 . We collected infected tissues three days after the infection. Leaf-disks of 28 mm diameter were collected per plant from 8-12 plants. Then, leaf-discs were placed in tubes containing metal beads and 500 mL of MgSO 4 and ground using Geno-Grinder (SPEX). Suspensions of 20 mL were transferred to a plate containing 180 mL of MgSO 4 and 106 serial dilutions were made. Aliquots of 10 mL for each serial dilution were then plated on KB medium with antibiotics and incubated at 30˚C for 2-3 days after which colony forming units were counted. Statistical analysis were performed using the Student's t test as described previously [24].

Hpa Emwa1 infection assay
Arabidopsis seedlings were grown for 10 days at 18˚C and 80-100% relative humidity before Hpa infection at dawn (0 hour) of growth chamber used. Tenday-old Arabidopsis plants were spray-inoculated (5610 5 spores per mL) with an asexual spore suspension of Hpa. Disease phenotypes were scored seven days postinfiltration (dpi) using trypan blue staining. A total of 60 infected cotyledons per genotype were used for sporangiophore (SPP) counting and statistical analysis were performed as described previously [25].

Time course SA treatment
Three-week-old plants were sprayed with 1 mM of salicylic acid and their leaves were collected at five different time points: when lights were turned on (0 hour), 4, 8, 16 and 24 hours. The leaves were immediately frozen in liquid nitrogen and stored at 280˚C for subsequent RNA extraction by Trizol method and cDNA synthesis followed by qRT-PCR time course analysis. Mock-treated control plants were sprayed with water and collected at the same time points as SA-treated ones.

RNA extraction and quantitative real-time PCR (qRT-PCR)
Total RNA was isolated using Trizol reagent (Ambion), and at least 2 mg of RNA were used for cDNA synthesis using SuperScript III reverse transcriptase (Invitrogen) according to the manufacturer's instructions. Amplicons were amplified from cDNA using gene specific primers (see Table 1), and their relative gene expression values were normalized to the ACTIN2 gene (invariant control) using the DDC T method [26]. Quantitative real-time PCR (qRT-PCR) was performed in a Mastercycler ep realplex S instrument (Eppendorf) using SYBR Green (Applied Biosystems) to monitor dsDNA synthesis.

NPR1 protein analysis
Total protein extracts were obtained from four-week-old Arabidopsis plant leaves either treated with SA (24 hours) or non-treated. Plant leaves were flash-frozen in liquid nitrogen and grinded using Geno-Grinder (SPEX). Cell debris was pelleted by centrifugation at 12,000 rpm for 10 min at 4˚C to obtain clear protein extracts. Total protein was extracted using plant extraction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM MgCl 2 , 5 mM EDTA, 0.1% Triton X-100, 0.2% Nonidet P-40, 6 mM beta-mercaptoethanol and 1 protease inhibitor cocktail (Roche) tablet per 100 mL of solution). Protein concentration for the various extracts were measured according to Bradford's method [27]. Aliquots of protein extract were separated on a 12% SDS-PAGE gel [28] and transferred to a nitrocellulose membrane. Western analysis was performed using an antibody against NPR1 protein as described previously [29].

Data analysis
All the measurements were performed with three biological replicates grown independently. Significant differences between data sets were evaluated using the Student's t test with GraphPad Prism software.

Results and Discussion
KO-nudx8 mutant displays a small, stunted photoperiod-dependent phenotype In order to identify genes involved in RPP4-mediated resistance against Hpa Emwa1, a time course gene expression profiling was performed after Hpa infiltration (0 hour, 2 days post-infiltration, 4 dpi and 6 dpi) for the rpp4 mutant and WT. This microarray data was submitted in NCBI's Gene Expression Omnibus database (GEO, accession number GSE22274) [30].
We found 106 differentially expressed genes between WT and rpp4. The Athena database (http://www.bioinformatics2.wsu.edu/Athena) was used to analyse the promoter regions to identify regulatory cis-acting elements for all the differentially expressed genes. Remarkably, it was identified that the most prominent ciselement present in the promoters of 30 genes analysed was the evening element (EE). The evening element is known to be regulated by the CIRCADIAN CLOCK-ASSOCIATED 1 gene (CCA1), and the fact the EE can be found in the promoter region of some defense genes suggests a link between the circadian clock and plant defense. It was previously reported that CCA1 transcription factor can control expression of several R genes [25], establishing a link between the circadian clock and plant immunity. We obtained T-DNA insertion mutants of these genes and profiled their disease susceptibility phenotype to Hpa infection and Pseudomonas syringae pv. maculicola (Psm) ES4326 followed by functional characterizations of susceptible mutants. Using PCR we genotyped a Salk T-DNA insertion line (SALK_092325.55.50.x) for the AtNUDX8 gene (AT5G47240) to confirm its homozygosity. The T-DNA insertion was located in exon 4 and position +963 relative to the start codon (  Fig. 1A) based on the sequence obtained from the left-border T-DNA primer of SALK_092325.55.50.x (http://signal.salk.edu/). We performed quantitative realtime PCR to measure the expression of AtNUDX8 gene in WT and homozygous KO-nudx8 mutant plants containing the T-DNA insertion, and we demonstrated that the expression of AtNUDX8 was abolished in the Salk mutant line (Fig. 1B).
Furthermore, we observed a small stunted phenotype for KO-nudx8 plants when grown on 12/12 hour photoperiod light (Fig. 2) similar to the AtNUDX7 gene. The same phenotype was not observed for short or long day light exposures (8/16 hours or 16/8 hours of light/dark).

KO-nudx8 plants exhibit a significant increase in susceptibility to pathogen infection
KO-nudx8 plants were significantly more susceptible to infection with oomycete pathogen Hpa Emwa1 in comparison with WT (Fig. 3A). Similarly, KO-nudx8 also exhibited a significant increase in bacterial growth of Psm ES4326 when compared to WT plants (Fig. 3B). Together, these results indicate that AtNUDX8 is involved in plant defense responses upon pathogen attack whereas plants without a functional copy of AtNUDX8 exhibit a disease susceptible phenotype. Moreover, AtNUDX8 transcript levels were found to be downregulated in the rpp4 mutant compared to WT plants from microarray data upon Hpa infection (GEO accession number GSE22274). This result indicates that the AtNUDX8 gene is involved in RPP4-mediated defense against Hpa. In addition to being susceptible to Hpa, we also demonstrated increased disease susceptibility in KO-nudx8 mutant for Psm ES4326 infection, suggesting a common defense mechanism for both types of pathogens analyzed in this study. All these factors indicate that AtNUDX8 acts as a positive regulator of defense in Arabidopsis. Members of the NB-LRR protein class can confer resistance to both Hpa and Psm. The idea that one gene in plant immunity may confer resistance against more than one pathogen type is not new [31] and it has recently been shown that pathogen effectors interact within a limited set of highly connected cellular hubs in plant immunity [32]. There is growing evidence, including for the gene presented in this study, that some defense genes are involved in plant resistance to more than one pathogen such as RPP4 and PgPR10-1 genes amongst others [31,32].
Additionally, in a similar way to the npr1 mutant, KO-nudx8 exhibited hypersensitivity to SA compared with WT control plants. Two-week-old KO-nudx8 Arabidopsis seedlings displayed impaired cotyledon germination in media with 0.2 mM SA compared to the WT control with the same conditions (  Fig. 4). Control plants in Murashige and Skoog (MS) medium without SA developed normally. When sucrose was subtracted from MS, the control plants grew normally in MS media without SA, but in 0.2 mM SA lacking sucrose plates there was a marked difference between WT and KO-nudx8 plants (Fig. 4A and 4B) whereby WT plants germinated cotyledons but KO-nudx8 barely germinated or developed cotyledons. These results suggest that the AtNUDX8 gene is involved in SA signaling and also, that in nutrient poor, energy deprived conditions AtNUDX8 is required for growth and detoxification.
Effect of disruption of the AtNUDX8 gene in pathogen induced thioredoxins TRX-h1, TRX-h2 and TRX-h3 NPR1 conformational changes in the cytosol can influence plant immunity. These conformational changes were shown to be modulated by TRXs that promote  . KO-nudx8 mutant is more susceptible to infection against bacterial and oomycete pathogen than WT plants. A) KO-nudx8 mutant is more susceptible to Hyaloperonospora arabidopsidis Emwa1 infection than the WT (three-fold change). Sporangiophore (SPP) count after Hpa Emwa1 infection at dawn. Ten-day-old Arabidopsis plants were inoculated (5610 5 spores per mL) with asexual spore suspension of Hpa. Disease phenotypes were scored seven days post-infiltration (dpi) after trypan blue staining. B) KO-nudx8 plants are more susceptible to the bacterial pathogen Psm ES4326 than WT (0.6 fold change Log 10 ). Infected tissue was collected three days after infection. All experiments were carried out with at least three biological replicates. Error bars represent 95% confidence intervals of log-transformed data (n57). Asterisks indicates significant difference according to Student's t test (P,0.05). NPR1 monomerization [4]. In order to verify the role of the AtNUDX8 gene in thioredoxin-dependent NPR1 oligomer-monomer reduction we analyzed the gene expression of three thioredoxins (TRX-h2, TRX-h3 and TRX-h5) which are known to be upregulated upon pathogen response. The transcript levels of all three pathogen-regulated thioredoxins were significantly reduced in KO-nudx8 plants at specific time points in SA-treated and untreated plants (Fig. 5A, 5B and 5C). This result indicates that AtNUDX8 can modulate expression of the three pathogen induced thioredoxins at specific time points. Further investigation is required to understand how the AtNUDX8 coding enzyme interacts with thioredoxins to modulate NPR1 conformational changes. AtNUDX8 interaction with thioredoxins could be by direct protein-protein interaction or by changing the levels of a metabolic intermediate by substrate hydrolysis that consequently will affect the redox pools in the cell. AtNUDX8 could act as a potential house-cleaning enzyme in the process of removing excessive reactive oxygen species (ROS) towards the end of a pathogen attack event. Mutations in the AtNUDX8 gene is likely to cause changes in redox homeostasis in the cell by affecting levels of a key metabolic intermediate impacting several plant physiological processes such as pathogen defense and SA biosynthesis. AtNUDX8 expression peaks at 8 hours and is significantly repressed four-fold by Salicylic Acid We examined AtNUDX8 transcript levels in a time course expression analysis, and we observed that this gene, unlike other Nudix homologs previously described involved in defense [21,22], displayed a typical circadian-like pulse gene expression with expression peaking at 8 hours after the lights were turned on (  Fig. 6). A time course expression profiling for AtNUDX8 from Diurnal database Functional Analysis of AtNUDX8 Shows a Role in Plant Immunity (http://diurnal.mocklerlab.org/) profiled similar results with AtNUDX8 expression also peaking at 8 hours (Zeitgeber time). Although AtNUDX8 transcript exhibited a rhythmic circadian-like expression, further experiments will be required to validate AtNUDX8 as a bona fide binding site for CCA1 or other circadian clock transcription factors by ChIP qRT-PCR or Yeast-One-hybrid experiments. Additionally, AtNUDX8 transcription was repressed four-fold at 8 hours in response to 1 mM SA treatment compared with mock-treated plants. Regulation of defense gene expression must be controlled between positive and negative regulators in order to avoid an overactivation of defense in plants, which would lead to impaired normal cell activity and loss of redox homeostasis in the cell.

Effect of disruption of AtNUDX8 in NPR1-mediated defense
To study the role of AtNUDX8 in NPR1-mediated defense and the SA signaling pathway, we performed a time course expression analysis to measure the transcript levels of NPR1, PR1 and the SA biosynthetic gene ICS1 in KO-nudx8 and WT plants. NPR1 transcript levels were significantly downregulated in KO-nudx8 plants at specific time points in SA-induced plants and untreated (no SA) (Fig. 7). PR1 gene expression was also reduced significantly at 8 hours in the KO-nudx8 mutant compared to WT in SA-induced plants (Fig. 8). Similarly, expression of the ICS1 gene was significantly repressed at 4 hours in untreated plants and at 16 and 24 hours in SA-treated plants in the KO-nudx8 mutant plants compared with the WT (Fig. 9). This downregulation of ICS1 suggests that AtNUDX8 is involved in the SA signaling pathway by indirectly acting on ICS1 transcript levels. SA is synthesized from chorismate through isochorismate by the action of ICS1. The fact that AtNUDX8 acts to modulate, not only thioredoxindependent NPR1 oligomer-monomer formation, but also ICS1 transcript levels, indicates that the AtNUDX8 gene can impact SA levels in the plant indirectly since NPR1 has been shown to maintain SA levels. NPR1 acts not only downstream to SA but also upstream as a negative regulator of ICS1 gene expression, inhibiting SA [7,33].
Furthermore, we did not detect NPR1 protein in the KO-nudx8 mutant without SA treatment (Fig. 10A), but we were able to detect with SA treatment. The fact that NPR1 protein was detected in the SA-treated KO-nudx8 mutant indicates that other pathways and genes can compensate for AtNUDX8 loss in SAdependent NPR1 signaling. A similar result was observed for NPR1 in nonreducing conditions with a marked decrease in NPR1 oligomer for the KO-nudx8 mutant compared to the WT plants without SA treatment (Fig. 10B). No difference in oligomer level was observed upon SA treatment. A previous study [21] demonstrated a role for an AtNUDX8 paralog, AtNUDX6, in NPR1-mediated defense and SA biosynthesis. Interestingly AtNUDX6 belongs to the same Nudix subfamily as AtNUDX8, grouping together in a monophyletic clade [18]. It has been shown that both AtNUDX6 and AtNUDX7 genes are positive and negative regulators of defense response respectively [21,22]. Our findings indicate a positive role for the AtNUDX8 gene in NPR1-mediated defense in Arabidopsis in a similar way to AtNUDX6, both antagonizing AtNUDX7's negative role in defense. Indeed, both AtNUDX8 and AtNUDX6 genes may act synergistically to promote plant immunity through the regulation of NPR1 conformation in the cytosol albeit by slightly different mechanisms. Contrarily to AtNUDX6, which is induced upon SA treatment, AtNUDX8 was repressed up to four-fold by SA when its expression was at its peak at 8 hours (see Fig. 5), indicating slightly different regulatory functions for these family member homologs upon SA signaling. A fine-tuning of both positive and negative regulation of defense related genes is required in order to avoid excessive activation of defense reaction upon pathogen infection.
Furthermore, we demonstrated in this study that AtNUDX8 transcript was significantly induced by ABA treatment (Fig. 11) indicating a possible functional role in abiotic stress responses such as osmotic stress, drought and salinity. Further investigations will be required to assess the involvement of the AtNUDX8   gene in such events. Microarray data from the eFP Arabidopsis browser (http:// bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi) corroborates this hypothesis as it shows that AtNUDX8 transcription is upregulated under several abiotic stress parameters such as osmotic stress, salt, drought, genotoxic and wounding.

Conclusions
This work lays the foundation for the study of a novel, previously uncharacterized member of the Nudix hydrolases, AtNUDX8 gene, in plant immunity and SA signaling in Arabidopsis. Plants without a functional copy of the AtNUDX8 gene were significantly more susceptible to infection than WT plants against Hpa and Psm ES4326 pathogen infection, indicating a positive role in plant defense for the AtNUDX8 gene. Additionally, KO-nudx8 plants displayed a small, stunted phenotype when grown over a 12/12 hour photoperiod (light/dark) but not over a 16/8 hour photoperiod. KO-nudx8 plants also displayed a phenotype of SA hypersensitivity compared to the WT control when grown in SA-containing media. All the three pathogen-responsive TRXs (TRX-h2, TRX-h3 and TRX-h5) were downregulated at specific time points in the KO-nudx8 mutant indicating that AtNUDX8 may have an important function in the oligermerization state of NPR1 indirectly by the modulation of pathogen responsive TRXs. We also demonstrated that KO-nudx8 mutant displayed significantly lower NPR1, PR1 and ICS1 transcription levels at specific time points compared with the WT indicating that AtNUDX8 plays a role in NPR1-dependent defense pathway and SA signaling. Collectively, these results demonstrate that the AtNUDX8 gene is involved in RPP4-mediated resistance by the modulation of pathogen responsive TRXs that, in turn, could cause changes in NPR1 reduction state. The precise mechanism of how AtNUDX8 modulates TRXs remains unknown. We speculate that AtNUDX8 is an important element linking redox or energy metabolism and biotic stress signaling by affecting levels of a yet unknown metabolic intermediate by substrate hydrolysis.
AtNUDX8 exhibited a typical circadian-like rhythmic expression peaking at 8 hours after the lights were turned on indicating a possible involvement of AtNUDX8 in light regulation or the circadian clock but further experiments will be necessary to determine AtNUDX8 as a bona fide target for core clock transcription factors such as CCA1 transcription factor. Indeed, out of the three Nudix members shown to be involved in plant immune responses thus far (AtNUDX8, AtNUDX6 and AtNUDX7), only AtNUDX8 has EE binding motifs on its promoter region.
The emerging picture indicates an active role for Nudix hydrolases in plant immunity and future work will be necessary to clarify our understanding of, not only AtNUDX8 gene, but also other Nudix family members in biotic and also abiotic stress such as drought, osmotic stress and wounding amongst others. These results argue for potential biotechnological applications of the AtNUDX8 gene in commercial plant systems for pathogen resistance and possibly abiotic stress tolerance, although further work will be required to assess the functional role of AtNUDX8 in abiotic stress responses. Future experiments such as enzyme activity assays will be important to know which substrate the AtNUDX8 enzyme affects. Global expression profile studies between KO-nudx8 mutant and WT will help to identify differentially expressed genes and pathways regulated by AtNUDX8. Additionally, differences in metabolite signatures detected by mass spectrometry between KO-nudx8 and WT plants will be useful in helping to identify which cell metabolites are affected by the AtNUDX8 enzyme.