Constitutively Active FOXO1 Diminishes Activin Induction of Fshb Transcription in Immortalized Gonadotropes

In the present study, we investigate whether the FOXO1 transcription factor modulates activin signaling in pituitary gonadotropes. Our studies show that overexpression of constitutively active FOXO1 decreases activin induction of murine Fshb gene expression in immortalized LβT2 cells. We demonstrate that FOXO1 suppression of activin induction maps to the −304/−95 region of the Fshb promoter containing multiple activin response elements and that the suppression requires the FOXO1 DNA-binding domain (DBD). FOXO1 binds weakly to the −125/−91 region of the Fshb promoter in a gel-shift assay. Since this region of the promoter contains a composite SMAD/FOXL2 binding element necessary for activin induction of Fshb transcription, it is possible that FOXO1 DNA binding interferes with SMAD and/or FOXL2 function. In addition, our studies demonstrate that FOXO1 directly interacts with SMAD3/4 but not SMAD2 in a FOXO1 DBD-dependent manner. Moreover, we show that SMAD3/4 induction of Fshb-luc and activin induction of a multimerized SMAD-binding element-luc are suppressed by FOXO1 in a DBD-dependent manner. These results suggest that FOXO1 binding to the proximal Fshb promoter as well as FOXO1 interaction with SMAD3/4 proteins may result in decreased activin induction of Fshb in gonadotropes.


Introduction
In mammalian reproduction, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) production from pituitary gonadotrope cells is critical for the regulation of gonadal functions such as steroidogenesis and gametogenesis [1,2]. Editor: Andrew Wolfe, John Hopkins University School of Medicine, United States of America LH and FSH are heterodimeric glycoproteins composed of a common alpha subunit and a beta subunit which is unique to each hormone [3]. Transcription of Lhb and Fshb is one of the rate limiting steps in the production of the mature hormones [4,5] and is tightly controlled by a complex network of hormonal signaling pathways including those activated by gonadotropin-releasing hormone (GnRH) and activin [6].
There is considerable evidence that gonadotropin production may be modulated by metabolic hormones such as insulin, in addition to reproductive hormones [22][23][24][25][26][27]. One group of candidate genes that may be regulated by insulin in gonadotropes is the FOXO subfamily of forkhead box transcription factors. FOXOs have been shown to be key regulators of cellular pathways involved in apoptosis, stress resistance, cell cycle arrest, and DNA damage repair [28,29]. They also have important roles in metabolism, homeostasis and reproduction. Foxo3 knockout mice have an age-dependent reduction in fertility caused by defective ovarian follicular growth, similar to premature ovarian failure in women [30]. Conditional knockouts of Foxo1 have demonstrated that FOXO1 plays a role in ovarian granulosa cell proliferation and apoptosis, along with FOXO3 and that FOXO1 is essential for maintenance and differentiation of spermatogonial stem cells in the testis [31,32]. The activity of FOXOs is regulated by post-translational modifications including phosphorylation, acetylation and ubiquitination [33]. Activation of the PI3K/AKT signaling pathway, in response to insulin/growth factor stimulation, results in FOXO phosphorylation, nuclear export and inhibition of their transcriptional activities [34].
Previously, we reported that the FOXO1 transcription factor is expressed in gonadotrope cells and that its phosphorylation and cellular localization are regulated by insulin signaling in a PI3K-dependent manner [35]. We also demonstrated that FOXO1 overexpression inhibits basal and GnRH induction of Lhb and Fshb synthesis in immortalized gonadotrope cells [35,36]. Since FOXO1 was reported to interact with SMAD3/4 in immortalized keratinocytes [37], we hypothesized that FOXO1 may also modulate activin signaling in gonadotrope cells. In this study, we used the immortalized gonadotrope-derived LbT2 cell model to determine whether FOXO1 alters activin induction of Fshb gene expression and to investigate the mechanisms involved.

Transient Transfection
LbT2 cells were seeded at 4.5610 5 cells/well on 12-well plates and transfected 18 hours later, using PolyJet DNA In Vitro Transfection Reagent (SignaGen, Rockville, MD), following the manufacturer's instructions. For all experiments, the cells were transfected for 6 hours with 400 ng of the indicated luc reporter plasmid and 200 ng of a b-galactosidase (b-gal) reporter plasmid driven by the Herpes Virus thymidine kinase promoter to control for transfection efficiency. The cells were switched to serum-free DMEM containing 0.1% BSA, 5 mg/L transferrin and 50 mM sodium selenite 6 hours after transfection. After overnight incubation in serum-free media, the cells were treated with vehicle (0.1% BSA) or 10 ng/mL activin (Calbiochem, La Jolla, CA) for 6 hours.

Luciferase and b-galactosidase Assays
To harvest the cells, they were washed with 16 phosphate buffered saline (PBS) and lysed with 0.1 M K-phosphate buffer pH 7.8 containing 0.2% Triton X-100. Lysed cells were assayed for luc activity using a buffer containing 100 mM Tris-HCl pH 7.8, 15 mM MgSO 4 , 10 mM ATP, and 65 mM luciferin. b-Gal activity was assayed using the Tropix Galacto-light assay (Applied Biosystems, Foster City, CA), according to the manufacturer's protocol. Both assays were measured using a Veritas Microplate Luminometer (Promega, Madison, WI).

Statistical Analyses
Transient transfections were performed in triplicate and each experiment was repeated at least three times as indicated in the figure legend. The data were normalized for transfection efficiency by expressing luc activity relative to b-gal and then made relative to the empty pGL3 plasmid to control for FOXO1 effects on the empty vector. The data were analyzed by Student's t-test for independent samples, one-way analysis of variance (ANOVA) followed by post-hoc comparisons with the Tukey-Kramer Honestly Significant Difference test or twoway ANOVA to determine synergy as described in [45] using the statistical package JMP 11.0 (SAS, Cary, NC). Significant differences were designated as p,0.05.

Quantitative RT-PCR
Total RNA was extracted from LbT2 cells with TRIzol Reagent (Life Technologies, Carlsbad, CA) following the manufacturer's protocol. Contaminating DNA was removed with DNA-free reagent (Life Technologies). 2 mg of RNA was reversetranscribed using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc., Hercules, CA) according to the manufacturer's protocol. Quantitative real-time PCR was performed in an iQ5 iCycler using iQ SYBR Green Supermix (Bio-Rad Laboratories, Inc.) and the following primers: Fshb forward, GCCGTTTCTGCA-TAAGC; Fshb reverse, CAATCTTACGGTCTCGTATACC; Gapdh forward, TGCACCACCAACTGCTTAG; Gapdh reverse, GGATGCAGGGATGATGTTC, under the following conditions: 95˚C for 5 min, followed by 40 cycles at 95˚C for 45 sec, 54˚C for 45 sec, and 72˚C for 45 sec. Each sample was assayed in triplicate and the experiment was repeated three times. Standard curves with dilutions of a plasmid containing Fshb or Gapdh cDNA were generated with the samples in each run. In each experiment, the amount of Fshb was calculated by comparing the threshold cycle obtained for each sample with the standard curve generated in the same run. Replicates were averaged and divided by the mean value of Gapdh in the same sample. After each run, a melting curve analysis was performed to confirm that a single amplicon was generated in each reaction.

Western Blot Analysis
Cells were harvested by incubating in a lysis buffer [10 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Nonidet P-40 (NP40), 1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride, complete protease inhibitor cocktail pellet (Roche Molecular Biochemical, Indianapolis, IN) and phosphatase inhibitor cocktail pellet (Roche)] for 10 min at 4˚C. The protein concentration was determined by Bradford assay. An equal amount of protein per sample was loaded on a 10% SDS-PAGE gel. Proteins were resolved by electrophoresis and transferred for 2 h at 100 V onto polyvinylidene difluoride membrane (Millipore, Billerica, MA). Membranes were blocked overnight in 5% nonfat milk, then incubated overnight at 4˚C with rabbit anti-human FOXO1 (1:1000; sc-11350) or rabbit anti-human GAPDH (1:3000; sc-25778). Blots were then incubated with an anti-rabbit horseradish peroxidaselinked secondary antibody (Santa Cruz Biotechnology) and bands were visualized using the SuperSignal West Dura Substrate (Thermo Scientific, Rockford, IL).

GST Interaction Assay
GST-SMAD2/3/4 were provided by Dr. Rik Derynck and the GFP expression vector by Dr. Douglass Forbes. 35 S-labeled proteins were produced using the TnT Coupled Reticulolysate System. Bacteria transformed with GST plasmids were grown to OD of 0.6 and induced with IPTG overnight at 30˚C [48]. Bacterial pellets were sonicated in 0.1% Triton X-100, 5 mM EDTA in 16 PBS, centrifuged and the supernatant was bound to glutathione sepharose 4B resin (Amersham Pharmacia Biotech, Piscataway, NJ). Beads were washed 46 in PBS and in HND buffer (10 mg/ml BSA, 20 mM Hepes pH 7.8, 50 mM NaCl, 5 mM DTT, and 0.1% NP-40). For the interaction assay, 20 ml of 35 S-labeled in vitro transcribed and translated GFP, FOXO1, FOXO1-CA, or FOXO1-CA-DBD mutant was added to the beads with 400 ml of HND buffer. Beads were incubated overnight at 4˚C, washed 26 with HND buffer and 26 with 0.1% NP-40 in PBS. Thirty ml of 26 Laemmli load buffer was added, the samples were boiled and electrophoresed on a 10% SDS-polyacrylamide gel. One fourth of the 35 S-labeled in vitro transcribedtranslated product was loaded onto the gel as input.

Co-Immunoprecipitation Assay
LbT2 cells were incubated overnight in serum-free media and then treated with or without 10 ng/mL activin for 2 hours. The cells were harvested and nuclear extracts were prepared, as previously described [49]. Protein concentration was determined by Bradford assay. 400 mg of pre-cleared nuclear extracts were incubated with 4 mg of mouse IgG (Santa Cruz sc-2025), SMAD4 antibody (sc-7966) or SMAD2/3 antibody (BD Biosciences 610842) at 4˚C for 1 hour. Twentyfive mL of Protein A Magnetic Beads (New England Biolabs, Ipswich, MA) were added and the extracts were rocked overnight at 4˚C. Bead/protein complexes were washed 16 with PBS then eluted in 26 SDS sample buffer at 70˚C for 5 minutes. 20 mg of protein was electrophoresed on a 10% SDS-PAGE gel, transferred to a polyvinylidene difluoride membrane and blocked overnight in 5% non-fat dry milk in 16 Tris-buffered saline with 0.1% Tween-20. The blots were then incubated overnight at 4˚C with rabbit anti-SMAD4 (Millipore 04-1033; 1:1000 dilution), SMAD2/3 (sc-8332; 1:1000) or FOXO1 (sc-11350, 1:1000 dilution) primary antibodies. Blots were incubated with a goat anti-rabbit horseradish peroxidase-linked secondary antibody (Santa Cruz; 1:5000) and bands were visualized using the SuperSignal West Dura Substrate (Thermo Scientific).

Constitutively Active FOXO1 Decreases Activin-Induced Fshb-luc
We recently published that overexpression of the FOXO1 transcription factor in immortalized LbT2 gonadotrope cells resulted in decreased basal and GnRHinduced Lhb and Fshb gene expression [35,36]. To determine whether FOXO1 can modulate activin signaling in gonadotropes, we transfected LbT2 cells with a multimer containing four repeats of a consensus FBE fused with a luc reporter gene (46FBE-luc) along with constitutively active FOXO1 (FOXO1-CA), which remains in the nucleus due to the inability of insulin/growth factor signaling to phosphorylate the mutated residues. Overexpression of FOXO1-CA increased expression of the 46FBE-luc but activin treatment did not result in significantly increased transcription of the 46FBE-luc in the absence or presence of FOXO1-CA (Fig. 1A). In contrast to the 46FBE-luc, overexpression of FOXO1 reduced expression of 21000 bp of the murine Fshb promoter fused to a luc reporter gene (mFshb-luc). As previously reported [36], both wild-type FOXO1 and FOXO1-CA reduced basal expression of mFshb-luc (Fig. 1B). Additionally, although the fold activin induction of the murine Fshb promoter was not significantly decreased by wild-type FOXO1, FOXO1-CA significantly reduced activin induction of Fshb by 50% (Fig. 1C). The lack of a significant decrease in activin induction of Fshb due to overexpression of wild-type FOXO1 was not altogether unexpected since we previously showed that transfection of LbT2 cells with pcDNA3 FOXO1 resulted in FOXO1 being predominantly localized in the cytoplasm with some nuclear localization whereas pcDNA FOXO1-CA was localized in the nucleus [36].

FOXO1 Decreases Activin-Induced Fshb mRNA Levels
To determine whether FOXO1-CA suppression of activin-induced transcription also occurs on the endogenous Fshb promoter in gonadotropes, we transfected LbT2 cells with a control GFP adenovirus (Ad-GFP) or an adenovirus containing FOXO1-CA (Ad-FOXO1-CA) and measured Fshb mRNA levels relative to Gapdh in response to vehicle, activin, GnRH, or activin and GnRH co-treatment. As previously reported [36], overexpression of FOXO1-CA significantly decreased basal Fshb mRNA levels by 62% (Fig. 1D). In LbT2 cells transduced with Ad-GFP, Fshb mRNA levels were induced 17 fold by activin and 4 fold by GnRH while cotreatment with activin and GnRH resulted in a synergistic 60 fold induction, similar to what was previously reported [50,51]. In contrast, activin induction was decreased by 62%, GnRH by 65% and activin and GnRH synergy by 82% in cells transduced with Ad-FOXO1-CA (Fig. 1D). These results indicate that constitutively active FOXO1 suppression of activin-, GnRH-or activin and GnRH-induced Fshb gene expression occurs in the context of the native chromatin.

FOXO1 Repression of Activin Induction Maps Between 2304 and 295 of the Fshb Promoter
As described in the introduction, the murine Fshb promoter contains multiple activin response elements including SMAD and FOXL2 binding sites ( Fig. 2A). We used 59 truncation analysis to determine which regions of the promoter were necessary for FOXO1 suppression. Both activin induction and FOXO1 suppression were lost with the 295 Fshb-luc (Fig. 2B). These results indicate that the region between 2304 and 295 is necessary for activin responsiveness as well as suppression by FOXO1 and suggest that the mechanism of suppression may involve SMAD and FOXL2 transcription factors. The results represent the mean ¡ SEM of three experiments performed in triplicate and are presented as luc/ b-gal. * indicates that the induction by FOXO1-CA is significantly different from EV using Student's t-test while n.s. indicates that the activin induction is not significantly different from vehicle. B-C. The 21000 murine Fshb-luc plasmid was transfected into LbT2 cells along with pcDNA3 EV, FOXO1 or FOXO1-CA (CA), as indicated. After overnight incubation in serum-free media, cells were treated for 6 h with 0.1% BSA veh or 10 ng/mL activin. The results represent the mean ¡ SEM of three experiments performed in triplicate and are presented as luc/bgal (B) or fold activin induction relative to vehicle control (C). * indicates that there is a significant activin induction compared to vehicle using Student's t-test (B). The different uppercase letters indicate that fold activin induction is significantly repressed by FOXO1-CA compared to EV using one-way ANOVA followed by Tukey's post-hoc test (C). D. LbT2 cells were transduced with a multiplicity of infection of 200 of Ad-GFP or Ad-FOXO1-CA for 6 hours, then switched to serum-free media. 24 hours after adenoviral infection, cells were treated with 0.1% BSA veh, 10 ng/mL activin, 10 nM GnRH, or both hormones for 6 hours, as indicated. The results represent the mean ¡ SEM of three experiments performed in triplicate and are presented as amount of Fshb mRNA relative to Gapdh. * indicates that Fshb transcription is significantly repressed by FOXO1-CA compared to Ad-GFP using Student's t-test while # indicates synergy between activin and GnRH activin using two-way ANOVA. The data concerning the effect of veh vs. GnRH was published previously [36].

FOXO1 DBD Is Required for Suppression of Fshb Gene Expression
To further investigate how activin-induced Fshb transcription is inhibited by FOXO1, we tested whether the FOXO1 DBD was necessary for the repression, as previously demonstrated for FOXO1 suppression of basal and GnRH-induced Lhb and Fshb gene expression [35,36]. As a control for the level of protein expression, we demonstrated that comparable levels of FOXO1-CA and a FOXO1-CA-DBD mutant were expressed when transfected into LbT2 cells (Fig. 3B). While FOXO1-CA overexpression in LbT2 cells suppressed activin-induced Fshb-luc, overexpression of FOXO1-CA with a DBD mutation (FOXO1-CA-DBD, Fig. 3A) was not able to repress activin induction of Fshb (Fig. 3C). These results indicate that the FOXO1 DBD is necessary to elicit an inhibitory effect on activin signaling to the Fshb promoter.

FOXO1 Binds to the Proximal Fshb Promoter
Since the FOXO1 repression mapped to the 2304/295 region of the Fshb promoter and required the FOXO1 DBD, we performed EMSA to determine whether FOXO1 could bind to this part of the promoter in vitro. Seven 35-mer oligonucleotide probes were designed to span the 2304/295 region. Flag-FOXO1-CA, synthesized with TnT rabbit reticulocyte lysate, bound to an oligonucleotide probe containing a consensus FBE (Fig. 4A, lane 1). To identify which complex contained the Flag-FOXO1-CA bound to the FBE, we supershifted the complex with a Flag antibody (Fig. 4A, lane 3) but not with control IgG (Fig. 4A, lane 2). Incubation with an oligo encompassing the 2125/291 region of the Fshb promoter also resulted in the formation of a barely detectable protein-DNA complex that was clearly shifted with a Flag antibody but not IgG (Fig. 4A, lanes 22-24) while incubation with oligos encompassing the 2305/2121 regions did not result in detectable FOXO1 binding (Fig. 4A, lanes 4-21). These results suggest that, in contrast to the consensus FBE, FOXO1 can bind weakly to the 2125/291 region of the murine Fshb promoter.

FOXO1 Interacts with SMAD3 and SMAD4
Since FOXO1 binding to the 2125/295 region of the Fshb promoter was weak compared to FOXO1 binding to the consensus FBE, we investigated whether FOXO1 physically interacts with SMAD proteins. We tested whether FOXO1 or DNA-binding deficient FOXO1 interacts with SMADs by incubating GST-SMAD2/3/4 fusion proteins with in vitro-transcribed and translated 35 S-labeled FOXO1, FOXO1-CA or a FOXO1-CA-DBD mutant in pull-down experiments. As shown in Fig. 5A, there was minimal interaction between the GST-SMAD fusion proteins and the negative control ( 35 S-GFP) or with GST alone incubated with FOXO1, FOXO1-CA or the FOXO1-CA-DBD mutant. In contrast, there was a strong interaction between FOXO1 and SMAD3 or SMAD4 which was not observed between FOXO1 and SMAD2. Additionally, a strong interaction was also observed between FOXO1-CA and SMAD3 or SMAD4 while there was no detectable interaction between the FOXO1-CA-DBD mutant and SMAD3 or SMAD4, indicating that the interaction between FOXO1 and SMAD3/4 requires the FOXO1 DBD.
Given our results demonstrating a direct protein-protein interaction between FOXO1 and SMAD3/4 in vitro, we then determined whether endogenous FOXO1 could interact with these proteins in gonadotrope cells using a co-immunoprecipitation assay. As shown in Fig. 5B, SMAD4 and SMAD2/3 were efficiently immunoprecipitated from nuclear extracts obtained from activin-treated or untreated LbT2 cells. Since SMAD4 has been previously shown to interact with SMAD2/3 [52], SMAD2/3 co-immunoprecipitated with SMAD4 or vice versa were used as positive controls and occurred in cells treated with activin. Interestingly, FOXO1 was also co-immunoprecipitated with SMAD4 or SMAD2/3 in an activindependent manner, indicating that endogenous FOXO1 can interact with SMAD3 and SMAD4 in gonadotropes.

FOXO1 Suppression of Activin-Induced Fshb Transcription Involves Inhibition of SMAD Transcription Factors
To further examine the mechanism of FOXO1 repression of activin induction, we tested whether FOXO1 could alter SMAD-dependent transcription in gonadotrope cells. Initially, we examined the effect of FOXO1-CA overexpression on SMAD induction of Fshb gene expression. Overexpression of SMAD3 or SMAD4 induced Fshb transcription by 3-4 fold while overexpression of SMAD3 and SMAD4 resulted in a 28-fold induction (Fig. 6A). Noticeably, FOXO1-CA overexpression resulted in a profound inhibition of SMAD3/4 induction of Fshb synthesis (Fig. 6A). We also demonstrated that the FOXO1 DBD was required for the suppression of SMAD3/4-induced Fshb transcription (Fig. 6B). Since FOXO1 suppression of activin-induced Fshb transcription mapped to the 2304/295 region of the Fshb promoter that contains multiple SMADs and FOXL2 binding sites, we tested whether activin induction of a multimer containing four repeats of a consensus binding element for SMADs or FOXL2 (SBE or FLRE) was inhibited by FOXO1. Activin induced the 46SBE-luc by 4 fold while the 46FLRE-luc was Western blot analysis was performed on whole cell extracts using FOXO1 and GAPDH primary antibodies and a horseradish peroxidase-linked secondary antibody. A representative image is shown. C. The 21000 murine Fshb-luc reporter was transfected into LbT2 cells along with pALTER EV, FOXO1-CA or FOXO1-CA-DBD mutant, as indicated. After overnight incubation in serum-free media, cells were treated for 6 h with 0.1% BSA or 10 ng/mL activin. The results represent the mean ¡ SEM of three experiments performed in triplicate and are presented as fold activin induction relative to the vehicle control. The different uppercase letters indicate that fold activin induction is significantly repressed by FOXO1-CA compared to EV or FOXO1-CA-DBD using one-way ANOVA followed by Tukey's post-hoc test.
doi:10.1371/journal.pone.0113839.g003 FOXO1 Decreases Activin Induction of Fshb induced by 1.5 fold (Fig. 6C). Interestingly, overexpression of FOXO1-CA significantly reduced the fold activin induction of the SBE but had no effect on the FLRE (Fig. 6C). Additionally, overexpression of FOXO1-CA with a DBD mutation was unable to suppress activin induction of the SBE, indicating that the FOXO1 DBD was also required for this effect. The weak activin induction of the 46FLRE and the lack of activin induction of a multimer of the 2350/2341 FOXL2 binding site [19] suggest that, unlike an SBE, a FOXL2 binding site is not sufficient for activin induction and thus, it is difficult to assess the role of FOXL2 in the FOXO1 suppression. In contrast, our data provides strong evidence that overexpression of FOXO1-CA results in decreased activin and SMAD-induction of Fshb in immortalized gonadotrope cells.

Discussion
The importance of activin signaling in the production of FSH has been illustrated using mouse knockout models. Deletion of the Type II activin receptor as well as gonadotrope-specific knockdown of both SMAD4 and FOXL2 resulted in a hypogonadal hypogonadism phenotype reminiscent of the Fshb knockout [53][54][55]. Since FOXO1 was previously reported to interact with SMAD3/4 proteins in HaCAT and Cos-1 cells [37], we investigated whether FOXO1 could regulate activin induction of Fshb gene expression. We demonstrated that overexpression of constitutively active FOXO1 repressed activin-or activin and GnRH-induced transcription of a murine Fshb-luc reporter as well as endogenous Fshb mRNA in LbT2 cells (Fig. 1). The FOXO1 repression occurred in a context-dependent manner since FOXO1 overexpression induced transcription of a consensus FBE β β Figure 5. FOXO1 Interacts with SMAD3/4. A. GST interaction assays were performed using bacterially expressed GST-fusion proteins (indicated above each lane) and 35 S-labeled in vitro transcribed and translated GFP, FOXO1, FOXO1-CA, and FOXO1-CA-DBD mutant (indicated on the left of the panel). GFP was used as a negative control. The GST-fusion proteins included GST alone, GST-SMAD2 (S2), GST-SMAD3 (S3), and GST-SMAD4 (S4). One quarter of the protein used in the interaction assay was loaded in the lane marked input. The experiment was repeated several times with similar results and a representative experiment is shown. B. Co-immunoprecipitation assays were performed using nuclear extracts from LbT2 cells treated with or without 10 ng/mL activin for 2 h after an overnight incubation in serum-free media. One tenth of the protein used in the immunoprecipitation reaction was loaded in the lane marked input. The immunoprecipitation was performed with mouse IgG, SMAD4 (S4) or SMAD2/3 (S2/3) antibody, as indicated. Western blot analysis was performed using SMAD4, SMAD2/3 and FOXO1 primary antibodies, and a horseradish peroxidase-linked secondary antibody. The experiment was repeated several times with similar results and a representative experiment is shown.
doi:10.1371/journal.pone.0113839.g005 FOXO1 Decreases Activin Induction of Fshb (Fig. 1A). Along with evidence that FOXO1 can suppress basal and GnRHinduced Fshb gene expression [36,56], these data support the idea that nuclear localized FOXO1 may have a significant inhibitory effect on Fshb transcription in gonadotrope cells although it should be noted that overexpression studies can result in responses that do not occur in the in vivo physiological context.
Since activin regulation of Fshb transcription involves both SMADs and FOXL2, we sought to characterize the mechanism that FOXO1 employs to repress activin signaling in gonadotropes. We demonstrated that the region between 2304 and 295, which contains several SMAD and FOXL2 binding elements, is necessary for the FOXO1 repression (Fig. 2). We then showed that two mutations (W209G and H215L) in helix 3 of the FOXO1 DBD prevented FOXO1 from eliciting a repressive effect (Fig. 3). H215 has been shown to make DNA contacts through hydrogen-bonding and water-mediated interactions [57] but the role of these two residues in protein-protein interactions is unknown. Our data suggests that the FOXO1 DBD is necessary for FOXO1 repression because FOXO1 binds directly to the Fshb promoter or because FOXO1 forms protein-protein interactions with factors critical for activin induction of Fshb transcription via the FOXO1 DBD.
To assess these two possibilities, we tested whether FOXO1 could bind to the region of the Fshb promoter necessary for the repressive effect. Gel-shift assays demonstrated that in vitro transcribed and translated FOXO1 bound to the 2125/ 291 region of the Fshb promoter, albeit much more weakly than FOXO1 binding to a consensus FBE (Fig. 4). Interestingly, the 2125/291 region contains a composite binding element for SMAD and FOXL2 proteins which has been shown to be essential for activin induction [16,20,21]. We did not observe binding of FOXO1 to this region of the promoter when LbT2 nuclear extracts were used instead of TnT FOXO1. Our studies are in agreement with a recent report by Choi et al. which demonstrated that FOXO1 can bind to the Fshb promoter using a DNA pulldown assay but was not detectable in a gel-shift assay employing LbT2 nuclear extracts [56]. Thus, these studies indicate that FOXO1 binding to the Fshb promoter may be a potential mechanism of FOXO1 repression of activin signaling in gonadotropes.
We then explored the possibility that FOXO1 repression of activin signaling in gonadotropes is due to protein-protein interactions between FOXO1 and SMAD3/4. Our GST pulldown experiments confirmed that FOXO1 can directly interact with SMAD3 and SMAD4 in vitro and that this interaction was dependent on the FOXO1 DBD (Fig. 5A). Notably, this was in contrast to the lack of interaction observed between FOXO1 and SMAD2. Since SMAD2 and SMAD3 are very similar proteins except that SMAD2 contains an insertion in the MH1 domain that prevents DNA binding, our data suggests that the inhibitory domain prevents interaction between FOXO1 and SMAD2. This idea is supported by the fact that the SMAD2 splice variant that lacks this insertion was reported to bind FOXO proteins [37]. It is also noteworthy that SMAD3 and SMAD4 were previously reported to bind FOXO1 through the MH1 domain [37]. FOXO1 interaction with SMAD3 and SMAD4 in an activin-dependent manner in Figure 6. FOXO1 Suppresses SMAD-Induced Fshb Gene Expression. A-B. The 21000 murine Fshb-luc plasmid was transfected into LbT2 cells along with 100 ng of pALTER empty vector (EV), FOXO1-CA or FOXO1-CA-DBD mutant, as well as 100 ng of pRK5 EV, 50 ng SMAD3 or SMAD4 with 50 ng of pRK5, or 50 ng SMAD3 and SMAD4 expression vectors, as indicated. Cells were incubated in serum-free media for 24 h before harvest. The results represent the mean ¡ SEM of three experiments performed in triplicate and are presented as fold SMAD induction relative to the pRK5 EV. * indicates that Fshb transcription is significantly repressed by FOXO1-CA compared to EV using Student's t-test (A) while the different uppercase letters indicate that Fshb transcription is significantly repressed by FOXO1-CA compared to EV or FOXO1-CA-DBD using one-way ANOVA followed by Tukey's post-hoc test (B). C. 46SBE-luc or 46FLRE-luc plasmids were transfected into LbT2 cells along with 200 ng of pALTER EV, FOXO1-CA or FOXO1-CA-DBD. After overnight incubation in serum-free media, cells were treated for 6 h with 0.1% BSA or 10 ng/mL activin. The results represent the mean ¡ SEM of three experiments performed in triplicate and are presented as fold activin induction relative to the vehicle control. The different uppercase letters indicate that transcription of the 46SBE-luc is significantly reduced by FOXO1-CA compared to EV or FOXO1-CA-DBD using one-way ANOVA followed by Tukey's post-hoc test while there is no significant difference in transcription of the 46FLRE-luc.
doi:10.1371/journal.pone.0113839.g006 FOXO1 Decreases Activin Induction of Fshb co-immunoprecipitation experiments suggests that SMAD phosphorylation is required to bind FOXO1 (Fig. 5B). This data is in agreement with the report that the FOXO1 interaction with SMAD3/4 was dependent on TGFb treatment of the cells [37]. Our studies also demonstrated that FOXO1 repressed the effects of SMAD3/4 overexpression on Fshb-luc and activin induction of a 46SBE-luc in a FOXO1 DBD-dependent manner (Fig. 6). Altogether, these results provide strong support for the hypothesis that FOXO1-CA modulates activin responsiveness of the Fshb promoter by interacting with SMAD3/4 via the FOXO1 DBD.
In summary, our studies provide evidence that the FOXO1 transcription factor may negatively regulate activin induction of Fshb synthesis through FOXO1 binding to the proximal Fshb promoter as well as through a direct interaction between the FOXO1 DBD and SMAD3/4. Since these experiments were performed in immortalized gonadotrope cells, additional studies are needed to determine whether FOXO1 functions in the pituitary to negatively regulate gonadotropin production in vivo. Moreover, since FOXO proteins act as coactivators of SMAD-dependent transcription in several other cell types [37,58,59], future studies are required to understand how FOXO1 acts as a repressor of activin signaling in pituitary gonadotrope cells. It may also be worthwhile to investigate whether FOXO1 regulates additional activin responsive genes in gonadotrope cells including the GnRH receptor and follistatin [60][61][62]. Interactions between FOXO and SMAD proteins may also be important for regulation of gene expression in other reproductive tissues that express both of these transcription factors such as the ovary and uterus [63,64].