Pesticide Methoxychlor Promotes the Epigenetic Transgenerational Inheritance of Adult-Onset Disease through the Female Germline

Environmental compounds including fungicides, plastics, pesticides, dioxin and hydrocarbons can promote the epigenetic transgenerational inheritance of adult-onset disease in future generation progeny following ancestral exposure during the critical period of fetal gonadal sex determination. This study examined the actions of the pesticide methoxychlor to promote the epigenetic transgenerational inheritance of adult-onset disease and associated differential DNA methylation regions (i.e. epimutations) in sperm. Gestating F0 generation female rats were transiently exposed to methoxychlor during fetal gonadal development (gestation days 8 to 14) and then adult-onset disease was evaluated in adult F1 and F3 (great-grand offspring) generation progeny for control (vehicle exposed) and methoxychlor lineage offspring. There were increases in the incidence of kidney disease, ovary disease, and obesity in the methoxychlor lineage animals. In females and males the incidence of disease increased in both the F1 and the F3 generations and the incidence of multiple disease increased in the F3 generation. There was increased disease incidence in F4 generation reverse outcross (female) offspring indicating disease transmission was primarily transmitted through the female germline. Analysis of the F3 generation sperm epigenome of the methoxychlor lineage males identified differentially DNA methylated regions (DMR) termed epimutations in a genome-wide gene promoters analysis. These epimutations were found to be methoxychlor exposure specific in comparison with other exposure specific sperm epimutation signatures. Observations indicate that the pesticide methoxychlor has the potential to promote the epigenetic transgenerational inheritance of disease and the sperm epimutations appear to provide exposure specific epigenetic biomarkers for transgenerational disease and ancestral environmental exposures.


Introduction
Epigenetic transgenerational inheritance is defined as the germline transmission of epigenetic information and phenotypic change across generations in the absence of any direct environmental exposure or genetic manipulation [1,2].Exposure of a gestating female (F0 generation) also exposes the F1 generation fetus and germline within the fetus that will generate the F2 generation, such that the F3 generation progeny is the first transgenerational generation with no potential exposure [2,3].The critical window of exposure for the germline is during fetal gonadal sex determination when epigenetic reprogramming in the primordial germ cell undergoes a DNA demethylation and remethylation [1].The environmental insults promote an apparent permanent alteration in the germline epigenome (DNA methylation) that escapes epigenetic reprogramming after fertilization, similar to an imprinted gene [4].This germline epigenetic inheritance will alter the embryonic stem cell epigenome such that all cell types derived will have an altered epigenome and transcriptome and those somatic cell types sensitive to this altered epigenome and gene expression will be susceptible to develop adult onset disease across generations [5,6].A number of previous studies have shown environmental toxicants including the fungicide vinclozolin [2,4], plastics (bisphenol A and phthalates) [7], pesticide (DEET and permethrin) [8], dioxin [9], hydrocarbons (jet fuel) [10], and dichlorordiphenyltrichloroethane (DDT) [11] promote the epigenetic transgenerational inheritance of adult onset disease and sperm epimutations [12].Interestingly, the transgenerational epigenetic alterations (epimutations) in sperm appear exposure specific and may be useful as biomarkers of ancestral toxicant exposure and susceptibility to develop transgenerational adult onset disease [12].
Methoxychlor is considered a model environmental endocrine disruptor with estrogenic and anti-androgenic activity [13].It has been used as an approved insecticide and pesticide to replace DDT for application on agricultural crops and livestock since its commercial production in the USA in 1946 [14].Contamination of food with methoxychlor has been previously observed [15].The toxic effects of methoxychlor in animal studies have been reviewed and they include adverse effects on fertility, early pregnancy and in utero development in females, as well as altered social behavior in males after prenatal exposure [13].A two-generation rat study examined methoxychlor's estrogenic and reproductive toxicity and found suppression of body weights, prolonged estrous cycles, reduced fertility, decreased numbers of implantation sites and newborns, decreased ovary weights, increased incidence of cystic ovary, increased uterine weights, delayed preputial separation, reduced sperm counts, and altered reproductive organ weights [16].Methoxychlor inhibits testosterone formation in rats [17,18,19,20], causes disruption of adult male reproductive function following transient exposure during sexual differentiation in rats [21,22], and alteration of mammary gland development in male rats [23,24].Induction of testis abnormalities in the F1 and F2 generations following exposure has been recorded with prenatal exposure to methoxychlor [25].Methoxychlor is carcinogenic for the liver, testis, ovary, spleen, blood vessels, pituitary, adrenals and mammary glands in rodents [26].Neonatal direct exposure to methoxychlor can influence pregnancy [27,28], ovarian and hypothalamic function [29,30], reproductive behavior [31], prostate development [32], thymus development [33], and testis development [34].Developmental methoxychlor exposure results in reduced ovulation and fertility and premature aging in rats [35] and altered reproductive and startle behaviors [36,37].Neuroendocrine effects of prenatal exposure to methoxychlor have been reported [38].A reduction in estrogen receptor beta gene expression in sheep hypothalamus was found after prenatal exposure to methoxychlor [39].Lifelong effects on neuroendocrine gene expression and premature reproductive aging occur following early life exposure to methoxychlor [38].In addition, epigenetic alterations in adult ovarian or hypothalamic genes are induced by fetal and neonatal direct exposure to methoxychlor [38,40].Exposure of gestating F0 generation females to methoxychlor during fetal gonadal sex differentiation in the rat caused transgenerational male testis effects including increased spermatogenic cell apoptosis, decreased sperm counts, and decreased sperm motility [2].Methoxychlor treatment of pregnant mice decreased the mean sperm concentrations by 30% and altered the methylation pattern of all the imprinted genes tested in the F1 offspring [41].Recently, methoxychlor has been shown to promote female reproductive disease and epigenetic changes in ovarian tissues and function [40,42,43].The toxicity profile of methoxychlor in humans revealed death, systemic (aplastic anemia), cardiovascular (low blood pressure), and neurological (blurred vision, dizziness and paresthesia) effects, and cancer (leukemia) [44].
The current study investigates methoxychlor's potential to promote the epigenetic transgenerational inheritance of adultonset disease in both males and females of subsequent generations following the transient exposure of an F0 generation gestating female during fetal gonadal sex determination.The parental origin of the germline transmission and germline epimutations are also investigated.This study used a dose of 200 mg/kg body weight for methoxychlor (4% of rat oral LD50) for administration to gestating rats.This is within the range of high environmental exposure dose for methoxychlor [45].No direct exposure toxic effects were anticipated in the current study or observed.The current study was not performed as a risk assessment study, but to investigate the potential and mechanisms involved in the transgenerational actions of methoxychlor.Previous studies demonstrated that the transgenerational actions of the pesticide DDT promoted obesity and other disease, so a comparison with the actions of methoxychlor was made [11].In the current study, diseases of the testis, prostate, kidney, ovary and uterus, as well as tumor development, abnormal puberty onset and obesity were evaluated in 10-12 month old F1 and F3 generation control and methoxychlor lineage rats.This study further documented sperm epimutations that are associated with adult onset disease and found a methoxychlor specific pattern of DNA methylation change in the sperm.These sperm epimutations in the F3 generation methoxychlor lineage are unique in comparison to a number of other exposure specific epimutation signatures including DDT.These epigenetic alterations may be useful as biomarkers of ancestral methoxychlor exposure and transgenerational adult-onset disease.

Transgenerational adult-onset disease analysis
The epigenetic transgenerational actions of methoxychlor administered to female rats during day 8 to 14 of gestation were investigated.This F0 generation transient exposure was the only exposure.F1 generation offspring were bred to generate the F2 generation which was bred to generate the F3 generation for both the vehicle dimethylsulfoxide (DMSO) control and methoxychlor lineages [2,3,4,7,8,9,10,11,12].No sibling or cousin breedings were used to avoid any inbreeding artifacts.The F1 and F3 generation rats of control and methoxychlor lineages were euthanized at 10-12 months of age.Body weights were measured and testis, prostate, kidney, ovary and uterus histopathology were examined.To assess if there were any toxic effects from embryonic exposure to methoxychlor the F1 generation body weights and organ weights were measured (Tables S1A and S1B).The body weights of the F1 and F3 generation methoxychlor lineage females were unaltered compared to controls.Following methoxychlor exposure the kidney weights in females and males of the F1 generation were increased.The ovarian and uterine weights were unaffected in the F1 generation.The testis weight decreased in the F1 generation methoxychlor lineage.The prostate, seminal vesicle and epididymal weights were unaltered in F1 generation.In addition, serum sex steroid hormone concentrations were measured in the F3 generation to assess any endocrine alteration.Serum estradiol concentrations in F3 generation females during diestrus phase or proestrus-estrus phase were unaffected (Figure S1).Serum testosterone concentrations in F3 generation males were unchanged (Figure S1, panel C).Observations indicate that overall there were no major F1 generation toxicity effects and no F3 generation endocrine effects from methoxychlor exposure.
The incidences of kidney disease in methoxychlor lineages are presented in Figure 1, panels A (females) and B (males) respectively.Kidney disease was characterized by either the presence of an increased number of proteinaceous fluid filled cysts or reduction in size of glomeruli or abnormality of Bowman's capsule thickness (Figure 1, panels C and D [control]; panels E and F [methoxychlor]).Previously these morphological abnormalities have been shown to be associated with an abnormal blood-urea-nitrogen (BUN) in the animals [46].There was an increase in kidney disease in F1 and F3 generation females of methoxychlor lineages (Figure 1, panel A).The F1 generation males of methoxychlor lineages showed an increase in kidney disease, while the F3 generation methoxychlor lineage males manifested an incidence of kidney disease that approached a significant increase (Figure 1, panel B).
The incidences of testis and prostate diseases in methoxychlor lineages are presented in Figure S2, panels A and B respectively.Testis disease was characterized by the presence of histopathology including azoospermic and atretic seminiferous tubules, presence of vacuoles in basal regions of seminiferous tubules, sloughed cells in the lumen of seminiferous tubules and lack of seminiferous tubule lumen (Figure S2, panel C [control]; panel E [methoxy-chlor]).There were no significant increases in testis disease in the F1 or F3 generation one year old males of methoxychlor lineages.To further study testis disease the number of apoptotic spermatogenic cells was examined by TUNEL analysis.The number of apoptotic spermatogenic cells did not significantly increase in the F1 or the F3 generation rats of methoxychlor lineages (Figure S3).Therefore spermatogenic defects that were present in vinclozolin lineage F3 generation rats [2] were not observed in F3 generation rats of methoxychlor lineages.Prostate disease was characterized by atrophic prostatic duct epithelium (Figure S2, panel D [control]; panel F [methoxychlor].The incidence of prostate disease also did not significantly increase in the F1 or the F3 generation males of methoxychlor lineage. The incidences of pubertal abnormalities in females (Figure 2, panel A) and males (Figure 2, panel B), ovary disease (Figure 2, panel C) and uterine infection (Figure 2, panel D) are presented.There was no increase in pubertal abnormalities in either the F1 or the F3 generation females or males of methoxychlor lineages.This included an assessment of both premature or delayed pubertal conditions as previously described [46,47].In contrast, the ovarian disease incidence increased both in the F1 and F3 generations of methoxychlor lineage (Figure 2, panel C).The ovarian disease was presented as a primordial follicle pool decrease or polycystic ovary disease [48].The primordial follicle ovarian pool was shown to have a reduction in the number of primordial follicles per ovary section.The polycystic ovarian disease was determined by an increase in the number of small and large cysts (Figure 2 panel F).In the F1 generation the ovarian disease in methoxychlor lineage was characterized by a primordial follicle pool decrease and a zero incidence of ovarian cysts.However, the F3 generation methoxychlor lineage ovarian disease was primarily characterized by the presence of ovarian cysts.The incidence of uterine infection did not significantly increase in the F1 or F3 generation rats of methoxychlor lineage (Figure 2, panel D).Uterine infection was determined by the enlargement of uterus, accumulation of foulsmelling dark discolored purulent material and presence of inflammation within the uterine horns [11].In addition, no significant change in uterine morphology was observed, as previously described [47].
The incidence of tumor development in females and males, and the incidence of obesity in females and males are shown in Figure 3.The primary tumors previously observed in transgenerational models were mammary gland tumors [46].There was no significant increase in tumor development in either females or males of the F1 and the F3 generation in methoxychlor lineage (Figure 3, panels A and B).The incidence of obesity in females and males did not increase in the F1 generation methoxychlor lineages (Figure 3, panels C and D).The incidence of obesity increased in females of the F3 generation of methoxychlor lineage (Figure 3, panel C).The incidence of obesity tended to increase in the F3 generation males of methoxychlor lineage (p,0.06; Figure 3 panel  D).The occurrence of obesity was determined by the excessive accumulation of subcutaneous and intra-abdominal adipose tissue and weight gain [11] (Figure 3, panel E).Although the average weight of all animals combined for a generation lineage was not altered, (Table S1), comparison of obese versus non-obese animal weights showed alterations.The mean weight of the methoxychlor lineage obese males (553610 g) and females (31769 g) was compared to mean weight of non-obese males (497612 g) and females (28468 g) respectively and found to be statistically different (p,0.05).
The incidence of diseases in individual rats from control and methoxychlor lineage is presented in Table S2A (F1 generation females), Table S2B (F1 generation males), Table S3A (F3 generation females) and Table S3B (F1 generation males).The incidence of total diseases per rat increased in both the F1 and the F3 generation females of methoxychlor lineage (Figure 4, panel A).The incidence of total disease per rat increased in the F1 generation males, but it did not increase in the F3 generation males of methoxychlor lineage (Figure 4, panel B).The incidence of multiple disease did not increase in the F1 generation females or males (Figure 4 panels C and D).The incidence of multiple diseases per rat increased in the F3 generation females of methoxychlor lineage (Figure 4, panel C).The incidence of multiple diseases also increased in the F3 generation males of methoxychlor lineage (Figure 4, panel D).Exposure to methoxychlor in F0 generation females therefore increased the overall incidence of adult onset diseases both in the F1 and F3 generation rats.Therefore, female gestating ancestors exposed to methoxychlor transmitted kidney disease, ovarian disease and obesity to their unexposed F3 generation descendants.Methoxychlor was found to induce the epigenetic transgenerational inheritance of adult-onset disease.
The parental origin of the germline transmission was investigated to determine if the epigenetic transgenerational inheritance of adult onset diseases is transmitted through the male or female germline [2,11].An outcross of a control or methoxychlor lineage F3 generation male with a wildtype female was examined to assess sperm transmission.Reverse outcrosses from the F3 generation control or methoxychlor lineage females with wildtype males were examined to assess oocyte transmission.The F4 generation outcross and reverse outcross offspring were maintained until the age of 10 months and disease analysis was performed and compared with the F3 generation animals (Tables S4, S5 and S6).The incidence of kidney disease in females (Figure 5, panel A), in males (Figure 5, panel B), obesity in females (Figure 5, panel C) and in males (Figure 5, panel D) in the F4 generation control and methoxychlor lineages are presented.The first set of graphs in each panel is from the outcross (OC) (F3 males bred with wild type females) and the second set of graphs in each panel is from the reverse outcross (ROC) (F3 females bred with wild type males).The incidence of kidney disease in the outcross F4 generation females of methoxychlor lineage was unaltered, while that of the reverse outcross females increased (Figure 5, panel A).Similarly, the incidence of kidney disease in the outcross F4 generation males of methoxychlor lineage was unchanged (Figure 5, panel B), but that of the reverse outcross males increased (Figure 5, panel B).The incidence of obesity in the F4 generation outcross and reverse outcross females of methoxychlor lineage were unaltered (Figure 5, panel C).The incidence of obesity in the F4 generation outcross males was unchanged while that of the reverse outcross males was increased (Figure 5, panel D).Therefore, the female obesity was not transmitted by either germline alone, but the male obesity was transmitted only through the female germline.
The incidence of total and multiple disease in the F4 generation outcross and reverse outcross offspring of the control and the methoxychlor lineages are shown in Figure 6 and Tables S5 and  S6.The incidence of the total disease in the F4 generation outcross females of the methoxychlor lineage were unaltered (Figure 6, panel A), but that of the reverse outcross females increased (Figure 6, panel A).The incidence of the total disease in the F4 generation outcross males of the methoxychlor was also unaltered but the incidence in the reverse outcross males were increased (Figure 6, panel B).The incidence of the multiple disease in the F4 generation outcross and reverse outcross females of the methoxychlor lineage tended to increase (Figure 6, panel C).The incidence of multiple disease in the F4 generation reverse outcross males of the methoxychlor were increased but that of outcross males did not increase (Figure 6, panel D).The incidence of testis disease, ovary disease, uterine disease, female and male pubertal abnormalities (i.e.premature or delayed) and tumors in both females and males were evaluated and there was no increase in any of these disease incidence in the F4 generation outcross or reverse outcross methoxychlor lineages (data not shown).A combination of both male and female exposure lineage germlines appear to be needed for these pathologies to manifest.Interestingly, observations indicate that the transmission of the increased incidence of kidney disease in both females and males, obesity in males, total disease in both females and males, and multiple disease in males occurs through the female germline.

Epigenetic Transgenerational Transmission of Sperm Epimutations
The methoxychlor induced epigenetic transgenerational inheritance of disease requires the germline transmission of epimuta-  tions.Previous studies have demonstrated F3 generation sperm develop differential DNA methylation regions (DMR) induced by a variety of different environmental toxicants [4,7,8,9,10,11], and these epimutation signatures are unique to the specific environmental exposure [12].The current study investigated the epimutations induced by methoxychlor in the F3 generation sperm.Three different experiments and comparisons with each involving different litters were used.The F3 generation control (DMSO) and methoxychlor lineage sperm were collected and DNA isolated for use in a methylated DNA immunoprecipitation (MeDIP) with an antibody to methyl-cytosine.The samples were pooled and then analyzed on a genome-wide rat promoter tiling array chip (MeDIP-Chip) as previously described [4,7,8,9,10,11,12].Those DMR in the methoxychlor lineage sperm that were statistically significant (p,10 25 ) from the control lineage sperm in a competitive hybridization, and were similar between all three different experiments, were identified and termed ''intersection'' epimutations with a total of 37 presented in Figure 7 and Table 1.These stringently selected intersection epimutations were the most reproducible between experiments and statistically significant.Using an average of three different experiments a total of 311 ''average'' epimutations were identified and their chromosomal locations are indicated in Figure 8.The list of all methoxychlor induced sperm epimutations are presented in Table S7.A number of the average epimutations appeared to cluster into similar chromosomal regions so a cluster analysis was performed as previously described [6] and a number of chromosomal regions were identified to have a statistically significant over-representation of epimutations in approximately two megabase regions with approximately five epimutations (Figure 8 and Table S8).These average epimutation clusters may represent regions of the chromosomes more sensitive to epigenetic regulation.
Previous studies have suggested the existence of exposure specific sets of sperm epimutations [12], so a comparison was made with a number of different exposure epimutation sets and the methoxychlor epimutations identified.A Venn diagram presented in Figure 9 indicates that the majority of methoxychlor intersection or average epimutations are unique with negligible overlap with the vinclozolin, pesticide (DEET and permethrin), DDT and plastics epimutations.No epimutations were found to be common between all the exposures.The highest overlap was observed between the plastic (BPA and phthalates) and pesticide (DEET and permethrin) as previously described [12], while for methoxychlor only 4 epimutations out of 37 intersection epimutations had any overlap (Figure 9).Therefore, the F3 generation transgenerational sperm epimutations induced by methoxychlor appear to be exposure specific.
Further analysis of the genes associated with the DMR in Table S7 demonstrate a number of functional gene pathways and cellular processes are potentially affected.Analysis of the association of the 311 average epimutations with functional signaling pathways and cellular processes used a KEGG pathway correlation as previously described [48].The pathways with a statistically significant overrepresentation of epimutation associated genes is shown in Table 2.
Therefore, the methoxychlor transgenerationally induced sperm epimutations have the potential to influence a variety of cellular pathways and processes.

Discussion
The current study demonstrated the pesticide methoxychlor can promote the epigenetic transgenerational inheritance of disease following the exposure of an F0 generation gestating female.The mechanism of this non-genetic form of inheritance involves the transgenerational transmission of adult-onset disease susceptibility through epigenetic changes in the germline.Although obtaining sufficient numbers of eggs was not possible to investigate oocyte differential DNA methylation regions, epigenetic alterations in the sperm DNA of the F3 generation (great-grand offspring) were observed after methoxychlor exposure of the F0 generation gestating female ancestors.This transgenerational transmission of adult onset diseases has implications of disease risk for not only the individual exposed, but also for future generations.
The toxicological profile of methoxychlor in humans includes death, systemic (aplastic anemia), cardiovascular (low blood pressure), and neurological (blurred vision, dizziness and paresthesia) effects, as well as cancer (leukemia) [44].Animal studies with methoxychlor documented adverse effects on fertility, early pregnancy and in utero development in females, and altered social behavior in males after prenatal exposure [13].A recent toxicity study revealed a suppression of body weights, prolonged estrous cycles, reduced fertility, decreased numbers of implantation sites and newborns, decreased ovary weights, increased incidence of cystic ovary, increased uterine weights, delayed preputial separation, and reduced sperm counts and reproductive organ weights [16].The current study examines the adult onset diseases in the direct exposed F1 generation fetus and in the transgenerational F3 generation offspring not exposed to methoxychlor.Although toxic methoxychlor doses were not used, the current study used intraperitoneal injection of a pharmacological dose based on 4% of the oral LD50 dose for methoxychlor.This dose is within the range of high environmental exposures [45].No major toxic effects were observed in F1 generation animals.However, the dose and route of administration used do not allow risk assessment of methoxychlor exposure to be assessed.The objective of the study was to further investigate [2] if exposure to methoxychlor could promote the epigenetic transgenerational inheritance of disease phenotypes and not designed to do risk assessment of exposure to methoxychlor.Future work with appropriate modes of administration and doses can now use the current information to more efficiently do risk assessment.The current study does reveal the potential of methoxychlor to promote the epigenetic transgenerational transmission of disease.
In this study the epigenetic transgenerational inheritance of disease observed includes kidney disease, ovary disease and obesity.The transgenerational kidney disease was observed in male and female F3 generation descendents of F0 generation gestating females exposed to methoxychlor.The transgenerational kidney pathology was similar to that previously observed [7,8,9,10,11,46] by other toxicants.In addition to the morphological defects, blood markers such as BUN alterations have previously been shown to support the presence of kidney disease [46].Cystic tubular nephropathy has been found following methoxychlor exposure in mice [49].Miniature swine given methoxychlor developed chronic renal disease in relatively short periods of time [26].Proximal convoluted kidney tubules showed vacuolar degeneration changes after methoxychlor exposure [50] Kidney disease in the F3 generation offspring following methoxychlor exposure to F0 generation gestating females in the current study highlights the risk of transgenerational transmission of kidney disease.
Ovarian diseases observed included increased rates of primordial follicle pool reduction associated with primary ovarian insufficiency (POI) and polycystic ovarian disease (PCO).An increase in POI was found in the F1 generation and increased PCO was found in the F3 generations of methoxychlor lineage females.Methoxychlor's toxicity effects on the ovary have been demonstrated and reviewed [13,16,25,35,38,40,42,51]. World-wide, human female populations are facing an increase in primary ovarian insufficiency characterized by primordial follicle pool loss, and the most common reproductive disease in women is polycystic ovarian disease characterized by the presence of anovulatory cysts [52,53].These ovarian disease phenotypes, as shown in the current study, may be in part the outcome of ancestral exposures, such as methoxychlor, and epigenetic transgenerational inheritance.In the current study the POI disease was observed in the direct exposure F1 generation and the polycystic ovarian disease was found in the transgenerational F3 generation.In previous transgenerational exposure models with a variety of different toxicants the POI has been observed in F1 and F3 generations [7,8,9,10,11,46,48], while PCO was primarily observed in the F3 generation females [48].Therefore, the absence of POI in the F3 generation methoxychlor lineage females was unique.The mechanism for the germline transmission of transgenerational disease involves a cell specific alteration in the epigenome and transcriptome of cells associated with disease.Previous observations have shown a transgenerational alteration in both the transcriptome and the epigenome of the ovarian granulosa cells from F3 generation vinclozolin lineage rats [48].In addition, epigenetic mechanisms underlie the development of polycystic ovary syndrome in women [54] and prenatally androgenized rhesus monkeys [55].Therefore, ancestral exposure to methoxychlor may contribute to the development of this ovarian disease through epigenetic transgenerational inheritance mechanisms.Observations suggest that this additional paradigm needs to be considered for the etiology of polycystic ovarian disease in women.
Methoxychlor was found to promote transgenerational obesity in approximately 25% of the females and 45% of the males of the F3 generation methoxychlor lineages in comparison to a 4% incidence in females and a 25% incidence in males in the F3 generation control lineage (Figure 3).Previously several environmental toxicants were found to promote the epigenetic transgenerational inheritance of obesity including plastics (BPA and phthalates) [7], hydrocarbons (jet fuel) [10], tributylin [56] and DDT [11].In addition to environmental toxicants promoting transgenerational obesity, nutrition is also a factor in the occurrence of transgenerational obesity [57,58,59,60].Obesity is increasing in the United States population and elsewhere in the world.According to the Center for Disease Control in 2010, 33% of adults in the United States are obese and 17% of children between the ages of 2-19 are obese [61].Obesity is a forerunner for many other diseases and the main adverse consequences of obesity are metabolic syndrome, cardiovascular disease, type 2 diabetes and a reduced average life expectancy [61,62].Obese women experience higher prevalence of amenorrhea, infertility and polycystic ovarian disease.Greater risks of pregnancy complications in these women such as hypertension, gestational diabetes and greater delivery problems occur, which may result in unplanned cesarean surgeries.Further, maternal obesity can have a negative effect on children's health [63].Experimental studies in rats show that obese dams transmit obesity to the subsequent generation [64].Waterland et al., (2008) [65] suggested that epigenetic mechanisms are involved in the transgenerational transmission of maternal obesity.Observations from the current study provide an example of an epigenetic transgenerational mechanism leading to an increase in obesity.The development of obesity as a result of ancestral exposure to environmental toxicants such as methoxychlor may be a component of the increase in obesity observed today.Future studies need to examine the adult status of obesity epigenetic markers, bone mineralization, adult bone length and metabolic disease in the F3 generation offspring of methoxychlor lineage to obtain insights into the pathogenesis of the adult-onset obesity observed.The suggestion that all these different disease phenotypes (kidney disease, ovary disease and obesity) may be linked to a complex disease syndrome that involves an environmentally induced epigenetic transgenerational inheritance mechanism needs to be considered as a potential component of the disease etiology.
Experiments were performed to determine the parental origin of the germline transmission of the transgenerational disease.
Observations of the increased incidence of disease in the F4 generation (female) reverse outcross offspring, but not in the (male) outcross offspring demonstrated transgenerational transmission through the female germline.This is one of the first studies showing a transmission of increased incidence of kidney disease in females and males, and obesity in males through the female germline after toxicant exposure to pregnant F0 generation females.Previous studies with vinclozolin [2] or high fat diet [57] showed transmission of increased incidence of disease through the male germline.Recently, DDT induced epigenetic transgenerational inheritance of obesity and other disease was found to involve a combination of male and female germline transmission transgenerationally [11].Interestingly, for DDT the male obesity was transmitted through the female germline and female obesity transmitted through the male germline [11].In the current study, methoxychlor male obesity was found to be transmitted through the female germline (Figure 5), but the female obesity was not observed in either the male or female germline transmission alone.Therefore, the transmission of transgenerational female obesity may involve a combination of male and female germline transmission.Previously, the optimal transgenerational transmission of disease appeared to involve a combined contribution of the male and female germline transmission [3,7,8,9,10,11].The current study demonstrates that female germline transmission of environmentally induced epigenetic transgenerational phenotypes is equally as stable as male germline transmission.Future studies will need to elucidate the sex specific roles and mechanisms involved.
One of the established mechanisms for the epigenetic transgenerational inheritance of disease and phenotypic variation is the reprogramming of the germline epigenome during gonadal sex determination [1,66].The germline epigenome (DNA methylation) is altered during gonadal development [67] and during gametogenesis appears to be permanently reprogrammed like an imprinted site and to be protected from DNA demethylation and reprogramming at fertilization in the following generations.The parent of origin of allelic transmission and differential DNA methylation programming suggest these epimutations are imprinted-like, but mono-allelic gene expression remains to be investigated.The transgenerational transmission of the modified germline epimutation results in modification of all somatic cell and tissue epigenomes and transcriptomes, promoting disease susceptibility and the epigenetic transgenerational inheritance of disease phenotypes [1].The current study investigated the transgenerational sperm epigenome and presence of epimutations induced in the methoxychlor lineage animals.The epigenetic analysis of the female germline was not possible due to the limited number of eggs available.Future studies will need to investigate the female germline, however, the current study focused on the male germline.The current study identified the differentially DNA methylated regions (DMR), defined as epimutations, in the methoxychlor lineage F3 generation sperm as previously described [12].A total of 37 intersection epimutations that were reproducible in all three experiments and 311 average epimutations were identified and associated with gene promoters.These epimutations were induced by the ancestral exposure to methoxychlor.The list of the DMR from methoxychlor lineage F3 generation sperm is presented in Table 1 and Table S7.The chromosomal locations of the transgenerational epimutations demonstrated that all chromosomes had epimutations and a number of the average epimutations were statistically over-represented in clusters of epimutations in regions of a two megabase size.These regions may be more susceptible to acquiring epimutations due to specific genomic features as previously described [4,6].Analysis of the genes associated with these epimutations demonstrated a wide variety of different functional gene pathways potentially effected have been previously correlated to disease (Table 2).Therefore, the observations support the presence of transgenerational sperm epimutations that will in part mediate the transgenerational inheritance of disease observed.Future studies will need to identify and compare the female germline epigenome modifications.
Environmentally induced epigenetic transgenerational inheritance of disease requires the absence of any direct exposure or genetic manipulation [1].Therefore, for gestating female exposures the F1 generation involves direct exposure and the F3 generation is the first transgenerational generation with no exposure [5].Environmentally induced pathologies such as uterine defects can promote pathologies in the offspring of the environmentally exposed individuals, as seen with diethylstilbesterol (DES) [68].However, in the absence of any environmental exposure the only mechanism to transmit information from one generation to the next is through the germline.In the case of epigenetic transgenerational inheritance this non-genetic form of inheritance transmits epimutations as observed in the current study.Although the later life adult onset disease phenotype may be secondarily derived from an early life abnormality such as uterine pathology or metabolic defects, the initial causal molecular event for the transgenerational phenotype is the germline epigenetic alteration.
An experiment with spermatogenic cell germline transplantation in a phthalate lineage transgenerational model [69] supports this requirement for the germline epigenetic modification for the transgenerational phenotype.
A variety of different environmental toxicants have been shown to induce the epigenetic transgenerational inheritance of disease and abnormal phenotypes [2,3,4,7,8,9,10,11,12,56,69,70].For example, gestational exposure to vinclozolin resulted in F3 generation disease including testis, prostate and kidney disease, immune system abnormalities, tumors, uterine hemorrhage during pregnancy, and polycystic ovarian disease [2,3,46,47].In addition, alterations in the methylation patterns of imprinted genes in sperm of the F3 generation male mice were found after vinclozolin exposure [70].Exposure of F0 generation gestating rats to Bisphenol-A resulted in reduced fertility in F3 generation males [71].Additional environmental factors such as nutrition [65,72] and stress [73] also can promote epigenetic transgenerational inheritance of disease phenotypes.Environmentally induced epigenetic transgenerational inheritance has been demonstrated in worms [74], flies [75], plants [76], fish [77], rodents [2] and mammals [78,79,80] indicating this phenomenon will likely be important in general biology and disease etiology [1].Observations demonstrate that exposure of gestating females to methoxychlor during gonadal sex determination promotes epigenetic transgenerational inheritance of adult-onset disease.These disease phenotypes have an impact on fertility and reproduction.The overall increase in total transgenerational disease and multiple disease are considerable.Interestingly, the increased disease incidence in F4 generation reverse (female) outcross offspring indicated that the transgenerational disease transmission was primarily through the maternal germline.Associated with the incidence of these transgenerational diseases are transgenerational epigenetic epimutations in the sperm DNA, while female germline epigenetic effects remain to be elucidated.These epimutations may be useful as early stage biomarkers of compound exposure and adult onset disease.Although not designed for risk assessment, these results have implications for the human population that is exposed to methoxychlor that may experience declines in fertility and increases in adult onset diseases with a potential to transmit these to subsequent generations.

Animal studies and breeding
Female and male rats of an outbred strain Hsd:Sprague Dawley, SD (Harlan) at about 70 and 100 days of age were fed ad lib with a standard rat diet and ad lib tap water for drinking.To obtain timepregnant females, the female rats in proestrus were pair-mated with male rats.The sperm-positive (day 0) rats were monitored for diestrus and body weight.On days 8 through 14 of gestation [47], the females were administered daily intraperitoneal injections of methoxychlor (200 mg/kg BW/day) or dimethyl sulfoxide (vehicle).Treatment lineages are designated control or methoxychlor lineages.The gestating female rats treated were designated as the F0 generation.The offspring of the F0 generation rats were the F1 generation.Non-littermate females and males aged 70-90 days from F1 generation of control or methoxychlor lineages were bred to obtain F2 generation offspring.The F2 generation rats were bred to obtain F3 generation offspring.Outcross F4 generation offspring (n = 8 litters per lineage) were obtained by breeding the F3 generation males from control and methoxychlor lineages with wild type females.Reverse outcross F4 generation progeny (n = 8 litters per lineage) were obtained by breeding the F3 generation females from control and methoxychlor lineages with wild type males.The outcross and the reverse outcross individuals were maintained until 10 months of age and then euthanized for tissue collection and disease evaluation.Onset of puberty was assessed in females by daily examination for vaginal opening from 30 days of age and in males by balano-preputial separation from 35 days of age.The F1-F3 generation offspring were not exposed directly to the methoxychlor treatment.All experimental protocols for the procedures with rats were preapproved by the Washington State University Animal Care and Use Committee (IACUC approval # 02568-031).

Tissue harvest and histology processing
Rats at 10-12 months of age were euthanized by CO 2 inhalation for tissue harvest.Body and organ weights were measured at dissection time.Testis, epididymis, prostate, seminal vesicle, ovaries, uterus and kidney were fixed in Bouin's solution (Sigma) and 70% ethanol, then processed for paraffin embedding by standard procedures for histopathology examination.Fivemicrometer tissue sections were made and were either unstained and used for TUNEL analysis or stained with H & E stain and examined for histopathology.Blood samples were collected at the time of dissection, allowed to clot, centrifuged and serum samples stored at 220uC for steroid hormone assays.

Testicular apoptotic cells by TUNEL
Testis sections were examined by Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay (In situ cell death detection kit, Fluorescein, Roche Diagnostics, Mannheim, Germany).Sections were deparaffinized and rehydrated through an alcohol series.They were deproteinized by Proteinase K (20 mg/ml; Invitrogen, Carlsbad, CA), washed with PBS and then 25 ml of the enzyme-label solution mix was applied to the sections and incubated at 37uC for 90 min.After PBS washes, slides were mounted and kept at 4uC until examination in a fluorescent microscope in dark field.Both testis sections of each slide were microscopically examined to identify and to count apoptotic germ cells by their bright fluorescence.

Histopathology examination and disease classification
Testis histopathology criteria included the presence of a vacuole, azoospermic atretic seminiferous tubule and 'other' abnormalities including sloughed spermatogenic cells in center of the tubule and a lack of a tubule lumen.Prostate histopathology criteria included the presence of vacuoles, atrophic epithelial layer of ducts and hyperplasia of prostatic duct epithelium.Kidney histopathology criteria included reduced size of glomerulus, thickened Bowman's capsule and the presence of proteinaceous fluid-filled cysts.A cut-off was established to declare a tissue 'diseased' based on the mean number of histopathological abnormalities plus two standard deviations from the mean of control tissues by each of the three individual observers scoring the tissues.This number was used to classify rats into those with and without testis, prostate or kidney disease in each lineage.A rat tissue section was finally declared 'diseased' only when at least two of the three observers marked the same tissue section 'diseased'.The occurrence of obesity was determined by statistically significant weight gain and excessive accumulation of subcutaneous and intra-abdominal adipose tissue.The proportion of rats with obesity, uterine disease or tumor development was obtained by accounting those that had these conditions out of all the animals evaluated.

Ovarian disease analysis by follicle and cyst counts
Every 30 th section of each pair of ovaries was stained with hematoxylin and eosin and three stained sections (150 mm apart) through the central portion of the ovary with the largest cross section were evaluated.Ovary sections were assessed for two diseases: primordial follicle loss and polycystic ovary disease.Primordial follicle loss was determined by counting the number of primordial follicles per ovary section and averaging across three sections.An animal was scored as having primordial follicle loss if the primordial follicle number was less than that of the control mean minus two standard deviations.Primordial follicles had an oocyte surrounded by a single layer of either squamous or both squamous and cuboidal granulosa cells [81,82].Follicles had to be non-atretic and showing an oocyte nucleus in order to be counted.Polycystic ovary was determined by microscopically counting the number of small cystic structures per section averaged across three sections.A polycystic ovary was defined as having a number of small cysts that was more than the control mean plus two standard deviations.Cysts were defined as fluid-filled structures of a specified size that were not filled with red blood cells and which were not follicular antra.A single layer of cells may line cysts.Small cysts were 50 to 250 mm in diameter measured from the inner cellular boundary across the longest axis.Percentages of females with primordial follicle loss or polycystic ovarian disease were computed.

Analysis of puberty onset
For identifying a rat with a pubertal abnormality the mean from all the rats in control lineage evaluated for pubertal onset was computed and its standard deviation calculated.A range of normal pubertal onset was chosen based on the mean 62 standard deviations.Any rat with a pubertal onset below this range was considered to have had an early pubertal onset and any rat with a pubertal onset above this range was considered to have had a delayed pubertal onset.The proportion of rats with pubertal abnormalities was computed from the total number of rats evaluated.

Overall disease incidence
A table of the incidence of individual diseases in rats from each lineage was created and the proportion of individual disease, total disease and multiple disease incidences was computed.For the individual diseases, only those rats that showed a presence of disease (plus) or absence of disease (minus) are included in the computation.For the total diseases, a column with total number of diseases for each rat was created and the number of plus signs were added up for each of the rats and the proportion was computed as the numbers of rats with total disease out of all the listed rats.For the multiple diseases, the proportion was computed as the number of rats with multiple disease out of all the listed rats.
Epididymal sperm collection, DNA isolation and methylated DNA immunoprecipitation (MeDIP) The epididymis was dissected free of connective tissue, a small cut made to the cauda and placed in 5 ml of F12 culture medium containing 0.1% bovine serum albumin for 10 minutes at 37uC and then kept at 4uC to immobilize the sperm.The epididymal tissue was minced and the released sperm centrifuged at 13,0006g and stored in fresh NIM buffer at 220uC until processed further.Sperm heads were separated from tails through sonication following previously described protocol (without protease inhibitors) [83] and then purified using a series of washes and centrifugations [84] from a total of nine F3 generation rats per lineage (control or methoxychlor) that were 120 days of age.DNA extraction on the purified sperm heads was performed as described [4].Equal concentrations of DNA from three individual sperm samples were used to produce three DNA pools per lineage and employed for chromatin immunoprecipitation of methylated DNA fragments (MeDIP).MeDIP was performed as previously described [4,12].

MeDIP-Chip Analysis
The comparative MeDIP-Chip was performed with Roche Nimblegen's Rat DNA Methylation 36720K CpG Island Plus RefSeq Promoter Array which contains three identical sub-arrays, with 720,000 probes per sub-array, scanning a total of 15,287 promoters (3,880 bp upstream and 970 bp downstream from transcription start site).Probe sizes range from 50-75 bp in length with the median probe spacing of 100 bp.Three different comparative (MeDIP vs. MeDIP) hybridization experiments were performed (3 sub-arrays) for methoxychlor lineage versus control, with each subarray encompassing DNA samples from 6 animals (3 each from methoxychlor and control).MeDIP DNA samples from experimental lineages were labeled with Cy3 and MeDIP DNA samples from the control lineage were labeled with Cy5.

Bioinformatic and Statistical Analyses of Chip Data
All the MeDIP-Chip raw hybridization data has been deposited in the NCBI GEO database (GEO # GSE58091) and is also available, along with the R-Code, used at www.skinner.wsu.edu.The data was imported into R (R Development Core Team (2010), R: A language for statistical computing, R Foundation for Statistical Computing.Vienna, Austria.ISBN 3-900051-07-0.URL http://www.R-project.org) and then annotation packages were built using pdInfoBuilder (Seth Falcon and Benilton Carvalho pdInfoBuilder: Platform Design Information Package Builder, R version 1.24.0) for platform design information.The oligo package [85] was used to read in the Nimblegen (XYS) files.The bioinformatic analysis was performed as previously described [4,12].The statistical analysis was performed in pairs of comparative IP hybridizations between methoxychlor (M) and controls (C) (e.g.M1-C1 and M2-C2; M1-C1 and M3-C3; M2-C2 and M3-C3).In order to assure the reproducibility of the candidates obtained, the intersection epimutations candidates showing significant changes in all of the single paired comparisons were examined as a having a significant change in DNA methylation between methoxychlor lineage and control lineage.This is a very stringent approach to select for changes, since it only considers repeated changes in all paired analysis.Alternately, the average epimutations obtained through an average of the three experiments (i.e.comparisons) were also examined.Clustered regions of interest were then determined by combining consecutive probes within 600 bases of each other, and based on whether their mean M values were positive or negative, with significance Pvalues less than 10 25 .The statistically significant differential DNA methylated regions were identified and P-value associated with each region presented.Each region of interest was then annotated for gene and CpG content.This list was further reduced to those regions with an average intensity value exceeding 9.5 (log scale) and a CpG density $1 CpG/100 bp.

Statistical analysis of rat organ and disease data
For statistical analysis, all of the data on body and organ weights were used as input in the program GraphPadß Prism 5 statistical analysis program and t-tests were used to determine if the data from the methoxychlor lineage differed from those of control lineages.For the number of rats with disease, logistic regression analysis was used to analyze the data (control or methoxychlor and diseased or unaffected).All treatment differences were considered significant if P value was less than 0.05.

Figure 1 .
Figure 1.Ancestral exposure to methoxychlor and transgenerational kidney disease.Percentages of females (panel A, C, E) and males (panel B, D, F) with kidney diseases in the F1 and F3 generation control (open bars) and methoxychlor (black bars) lineages.The number of diseased rats / total number of rats in each lineage are also shown (* P,0.05; ** P,0.01).doi:10.1371/journal.pone.0102091.g001

Figure 2 .
Figure 2. Ancestral exposure to methoxychlor and transgenerational pubertal abnormalities, and ovarian and uterine diseases.Percentages of females F1 and F3 generation (panel A) and males (panel B) with pubertal abnormality or females with ovarian disease (panel C) or uterine infection (panel D).Percentages of the F1 and F3 generation females with primordial follicle loss (panel E) and polycystic ovary disease (panel F) in control and methoxychlor lineages.The number of diseased rats / total number of rats in each lineage are also shown.(** P,0.01; *** P,0.001).doi:10.1371/journal.pone.0102091.g002

Figure 3 .
Figure 3. Ancestral exposure to methoxychlor and transgenerational tumor development and transgenerational obesity.Percentages of F1 and F3 generation females (panel A) or males (panel B) with tumor development and percentages of females (panel C) or males (panel D) with obesity.Representative abdominal adiposity for obese (panel E) animals is presented with arrows indicating the excessive dorsal abdominal and retro-peritoneal adiposity distribution.The number of diseased rats / total number of rats is shown above the respective bar graphs (** P,0.01).doi:10.1371/journal.pone.0102091.g003

Figure 4 .
Figure 4. Ancestral exposure to methoxychlor and adult-onset transgenerational disease in rats.Incidences of F1 and F3 generation total female disease (panel A), total male disease (panel B), female multiple disease (panel C) and male multiple disease (panel D) in control and methoxychlor lineages.The number of diseased rats / total number of rats is shown above the respective bar graphs (* P,0.05; ** P,0.01; *** P, 0.001).doi:10.1371/journal.pone.0102091.g004

Figure 5 .
Figure 5. Ancestral exposure to methoxychlor promoted adult-onset transgenerational diseases in F4 generation reverse outcross offspring showing female germline transmission.Incidences of kidney disease in females (panel A), in males (panel B), obesity in females (panel C) and in males (panel D) of the F4 generation outcross (OC) or reverse outcross (ROC) offspring of the control and methoxychlor lineages.doi:10.1371/journal.pone.0102091.g005

Figure 6 .
Figure 6.Ancestral exposure to methoxychlor promoted adult-onset transgenerational diseases in F4 generation reverse outcross offspring increasing total female disease, total male disease and multiple male disease incidences.Incidences of total disease in females (panel A), in males (panel B), multiple female disease (panel C), and multiple male disease (panel D) in the F4 generation outcross (OC) or reverse outcross (ROC) offspring of the control and methoxychlor lineages.doi:10.1371/journal.pone.0102091.g006

Figure 7 .
Figure 7. Ancestral exposure to methoxychlor and transgenerational epigenetic changes and induced sperm intersection epimutations.Chromosomal locations for regions with transgenerational change in DNA methylation (arrowheads).There were 37 differentially methylated regions in sperm DNA from methoxychlor lineage rats compared to control lineage rats for all three experiments.doi:10.1371/journal.pone.0102091.g007

Figure 8 .Figure 9 .
Figure 8. Ancestral exposure to methoxychlor and transgenerational epigenetic changes and induced sperm average epimutations.Chromosomal locations for regions with transgenerational change in DNA methylation (arrowheads).There were 311 differentially methylated regions in sperm DNA from methoxychlor lineage rats compared to control lineage rats for all experiments.The box under the line at each chromosome represents a statistically significant over-representation (cluster) of epimutations.doi:10.1371/journal.pone.0102091.g008

Figure
Figure S1 Transgenerational animal hormone levels.A. Serum estradiol concentrations in proestrus-estrus in F3 generation control and methoxychlor lineage females.B. Serum estradiol concentrations in diestrus in F3 generation control and methoxychlor lineage females.C. Serum testosterone concentrations in the F3 generation control and methoxychlor lineage males.(PDF) Figure S2 Ancestral exposure to methoxychlor and adult-onset transgenerational testis disease.Percentages of the F1 and F3 generation males of control and methoxychlor lineages with testis disease (panel A) or prostate disease (panel B).The number of diseased rats / total number of rats is shown above the respective bar graphs.Micrographs (scale bar = 100 mm) show testis disease (control: panel C; methoxychlor: panel E) and prostate disease (control: panel D; methoxychlor: panel F) in F3 generation rats.(PDF) Figure S3 Testicular spermatogenic cell apoptosis.Number of apoptotic germ cells in F1 and F3 generation control (open bars) and methoxychlor (black bars) lineages evaluated by TUNEL assay.(PDF)TableS1(A) Body Weight and organ weights in F1 and F3 generation female rats of Control and Methoxychlor lineages (mean 6 standard error).Asterisks (*, **, ***), if present, indicate statistically significant differences between means of Control and Methoxychlor lineages (P,0.05,P,0.01 and P,0.001 respectively); nd = not determined.(B) Body weight (grams) and organ weights (% of body weight) in F1 and F3 generation male rats of Control and Methoxychlor lineages (mean 6 standard error).Asterisks (*, **), if present, indicate statistically significant differences between means of Control and Methoxychlor lineages (P,0.05,P,0.01 respectively); nd = not determined.(PDF)
*Statistical likelihood of finding this many or more DMR associated genes in this pathway by Fisher's Exact Test.doi:10.1371/journal.pone.0102091.t002

Table S2 (
A) Individual disease incidence in F1 generation female rats of Control and Methoxychlor lineages.(B) Individual disease incidence in F1 generation male rats of Control and Methoxychlor lineages.(PDF) Table S3 (A) Individual disease incidence in F3 generation female rats of Control and Methoxychlor lineages.(B) Individual disease incidence in F3 generation male rats of Control and Methoxychlor lineages.(PDF) Table S4 (A) Body Weight in F4 generation Outcross and Reverse Outcross female rats of Control and Methoxychlor lineages (mean 6 standard error).(B) Body weight (grams) in F4 generation Outcross and Reverse Outcross male rats of Control and Methoxychlor lineages (mean 6 standard error).(PDF) Table S5 (A) Individual disease incidence in F4 generation Outcross female rats of Control and Methoxychlor lineages.(B) Individual disease incidence in F4 generation Outcross male rats of Control and Methoxychlor lineages.(PDF)

Table S6 (
A) Individual disease incidence in F4 generation Reverse Outcross female rats of Control and Methoxychlor lineages.(B) Individual disease incidence in F4 generation Reverse Outcross male rats of Control and Methoxychlor lineages.(PDF) Table S8 Characteristics of the average epimutation clusters and associated genes.Clusters with the same gene listed more than once indicates multiple epimutations associated with that gene.(PDF)