Selective Enrichment Yields Robust Ethene-Producing Dechlorinating Cultures from Microcosms Stalled at cis-Dichloroethene

Dehalococcoides mccartyi strains are of particular importance for bioremediation due to their unique capability of transforming perchloroethene (PCE) and trichloroethene (TCE) to non-toxic ethene, through the intermediates cis-dichloroethene (cis-DCE) and vinyl chloride (VC). Despite the widespread environmental distribution of Dehalococcoides, biostimulation sometimes fails to promote dechlorination beyond cis-DCE. In our study, microcosms established with garden soil and mangrove sediment also stalled at cis-DCE, albeit Dehalococcoides mccartyi containing the reductive dehalogenase genes tceA, vcrA and bvcA were detected in the soil/sediment inocula. Reductive dechlorination was not promoted beyond cis-DCE, even after multiple biostimulation events with fermentable substrates and a lengthy incubation. However, transfers from microcosms stalled at cis-DCE yielded dechlorination to ethene with subsequent enrichment cultures containing up to 109 Dehalococcoides mccartyi cells mL−1. Proteobacterial classes which dominated the soil/sediment communities became undetectable in the enrichments, and methanogenic activity drastically decreased after the transfers. We hypothesized that biostimulation of Dehalococcoides in the cis-DCE-stalled microcosms was impeded by other microbes present at higher abundances than Dehalococcoides and utilizing terminal electron acceptors from the soil/sediment, hence, outcompeting Dehalococcoides for H2. In support of this hypothesis, we show that garden soil and mangrove sediment microcosms bioaugmented with their respective cultures containing Dehalococcoides in high abundance were able to compete for H2 for reductive dechlorination from one biostimulation event and produced ethene with no obvious stall. Overall, our results provide an alternate explanation to consolidate conflicting observations on the ubiquity of Dehalococcoides mccartyi and occasional stalling of dechlorination at cis-DCE; thus, bringing a new perspective to better assess biological potential of different environments and to understand microbial interactions governing bioremediation.


Introduction
Dehalococcoides mccartyi is a newly classified genus and species belonging to the Dehalococcoidia class in the phylum Chloroflexi [1]. The members of this genus respire halogenated compounds with an array of carbon backbones of biogenic and anthropogenic origin (i.e., ethenes [2,3], benzenes [4], phenols [5], and biphenyls [6]). Of particular importance for bioremediation are Dehalococcoides mccartyi strains that reduce the widespread soil and groundwater contaminants perchloroethene (PCE), trichloroethene (TCE), and the daughter chlorinated products, cisdichloroethene (cis-DCE) and vinyl chloride (VC) to the nontoxic, non-chlorinated end product, ethene [1][2][3]7]. These strains couple the reductive dechlorination of these chlorinated ethenes to growth using H 2 as electron donor and acetate as carbon source [1]. Hence, stimulation of indigenous Dehalococcoides (biostimula-tion) or addition of laboratory-cultivated consortia containing Dehalococcoides (bioaugmentation) are bioremediation engineering avenues utilized to decontaminate and restore sites polluted with chlorinated ethenes [8,9]. The potential for partial reduction of PCE and TCE to cis-DCE extends to multiple bacterial genera [10]. On the other hand, the capacity to dechlorinate chlorinated ethenes to ethene is, to date, unique to Dehalococcoides mccartyi.
A range of (mostly) contaminated environments has served as microbial inocula for Dehalococcoides enrichment cultures throughout the two decades of research on reductive dechlorination. Table 1 contains a compilation of cultures employed in fundamental studies and in bioaugmentation research/applications for PCE or TCE dechlorination. Development of these enrichment cultures is a lengthy process [11] as the enrichments must be actively fed and transferred to maintain the desired biological activity. Careful consideration is given to any environmental sample (soil, sediment, or groundwater) before pursuing this labor-and time-intensive work. Often crucial in deciding to i) develop novel reductively dechlorinating enrichment cultures, ii) biostimulate, and iii) bioaugment a contaminated site is evidence of reductive dechlorination to VC and ethene. Hence, VC and ethene are measured either in laboratory microcosm experiments or directly, during evaluation of contaminated sites [12][13][14]. This information is not always reported per se; however, for the majority of the enrichment cultures in Table 1, there was evidence of desired biological activity through one or both assessment methods.
Observations on the presence of Dehalococcoides and the stalling of PCE/TCE dechlorination at cis-DCE or VC are also common. This puzzling outcome has been reported in soil and sediment microcosm studies and in bench-scale bioremediation scenarios [12,16,[25][26][27][28], as well as at contaminated sites undergoing bioremediation [8,14,29]. Whereas some of the abovementioned works did not put forth an explanation on the inability to achieve dechlorination of cis-DCE or VC, the most commonly proposed reason was the absence of Dehalococcoides mccartyi strains with DCEand VC-respiring metabolic capabilities [8,16,26,28,30]. Nonetheless, this unpredicted outcome was also noted even when the identified Dehalococcoides mccartyi genes vcrA and bvcA coding for VC reductive dehalogenase enzymes were detected [27]. Yet, neither VC reduction nor increases in Dehalococcoides mccartyi occurred in microcosms biostimulated with a fermentable substrate as the precursor for H 2 and acetate [27].
We hypothesize that, often, the discrepancy between the expected and the observed activities of Dehalococcoides is not due to their metabolic potential; instead, it is a consequence of the intrinsic competition for electron donor (H 2 ) in soils and sediments, driven by a variety of electron acceptors such as nitrate, Fe (III), sulfate, and bicarbonate (HCO 3 2 ). Electron donor competition was recognized early on as an important phenomenon that needed to be characterized in order to predict, explain, and optimize reductive dechlorination by Dehalococcoides [12,[31][32][33]. This need led to a number of studies showing that donorcompeting terminal electron accepting processes affect not only the rates but also the extent of reductive dechlorination of chlorinated ethenes [31,32,[34][35][36][37][38][39][40]. At contaminated sites undergoing in situ bioremediation, these processes could lead to minimal biostimulation of Dehalococcoides, prolonged lag times before the onset of dechlorination, and/or incomplete dechlorination. But despite the acknowledgement of donor competition as a variable in reductive dechlorination, its importance is often dismissed at field sites on the grounds that fermentable substrates responsible for H 2 production are added in excess [13].
In this study, microcosms were biostimulated with TCE and fermentable substrates to promote growth of Dehalococcoides mccartyi but stalled at cis-DCE, irrespective of the fact that electron donor was supplied ,150 times in stoichiometric excess for complete dechlorination of TCE to ethene. Our findings strongly support electron donor competition for the inability to produce VC and ethene in microcosms stalled at cis-DCE. Transfers from stalled microcosms in the absence of soil/sediment produced ethene and

Ethics Statement
No specific permits were required for the sampling activities in Cuzdrioara, Romania and Carolina, Puerto Rico. The Cuzdrioara location is privately-owned by the family of the first author (AGD). Please contact AGD for future permissions. The sampling location in Carolina is not privately-owned or protected in any way. The core sediments from Parris Island Marine Corps Recruit Depot (MCRD) Site 45 were donated by Paul C. Johnson from Arizona State University who was authorized by MCRD to collect these samples from their site. The sampling work did not involve any endangered or protected species in either location.

Environmental Sources
The soil and sediment samples originated from the following geographic locations: Cuzdrioara, Cluj County, Romania ( [41]. Once brought to the laboratory, all soils and sediments were stored at 4uC until the establishment of microcosms.

Microcosms and Enrichment of Soil/Sediment-Free, Chlorinated Ethene-Respiring Dehalococcoides Cultures
We established the following microcosms: Cuzdrioara soil, n = 3; Carolina sediment, n = 3; and Parris Island sediment, n = 20 (replicates from 10 core sections evenly dispersed throughout a 5 m depth profile) in 30 mM HCO 3 2 -buffered, reduced anaer-  obic mineral medium described [25]. Each microcosm consisted of 5 g soil or sediment in 160-mL glass serum bottles with 100 mL medium. The initial pH of the medium was 7.8. We added to each microcosm 0.2-0.3 mmol L 21 TCE (nominal concentration). Additionally, we added the fermentable substrates lactate (5 mM) and methanol (12 mM) as H 2 and acetate precursors, 1 mL ATCC MD-VS vitamin supplement (ATCC, USA), and 50 mL of vitamin B 12 from a 1 g L 21 stock solution. The microcosm bottles were incubated statically at 30uC. Cuzdrioara and Carolina microcosms were incubated for 200 days, during which time 5 mM lactate was re-added on two separate occasions (days 46 and 180).
We performed serial transfers (10% vol/vol) into same size serum bottles using the same medium compositions to remove the soil or sediment. The microcosm bottles were shaken vigorously and allowed to settle for 15 minutes so that the supernatant was mostly devoid of soil or sediment when transferred to the new bottles. Upon each transfer, we supplied additional TCE, lactate, and methanol. After three consecutive transfers, we named the enrichment culture from Cuzdrioara soil, ZARA-10, from Carolina sediment, LINA-09, and from Parris Island sediment, ISLA-08. At all times when handling these cultures, we employed aseptic technique and took precautions to avoid cross-contamination of the cultures. We maintained the three enrichment cultures by feeding them 3-4 times consecutively with 0.5 mmol L 21 TCE, 5 mM lactate, and 12 mM methanol or 1 mmol L 21 TCE, 5 mM lactate, and 24 mM methanol. Each addition of TCE was allowed to proceed to $80% ethene. We flushed the headspace of the bottles with ultra-high purity N 2 gas to remove headspace gases that accumulated as a result of dechlorination (ethene and VC), fermentation (CO 2 ) and methanogenesis (CH 4 ) before adding a new dose of TCE and fermentable substrates. Removal of CO 2 from the headspace would also raise the pH of the medium, which decreased as a result of dechlorination and fermentation. When not actively used in experiments, we kept stock cultures of the soil/ sediment-free enrichments in a 4uC refrigerator. No significant loss of activity was observed in these cultures even after several months of storage at 4uC, consistent with published findings [40].

ZARA-10 and LINA-09 Bioaugmentation Experiments
Bioaugmentation experiments were carried out to evaluate whether ZARA-10 and LINA-09 soil/sediment-free cultures could dechlorinate TCE to ethene in the soil and sediment from which they originated. We setup 120-mL glass serum bottles (four bottles per culture) with 2.5 g soil or sediment, 50 mL reduced anaerobic medium, 0.25 mmol L 21 TCE, 5 mM lactate and 12 mM methanol, and 1% inoculum vol/vol (0.5 mL) ZARA-10 or LINA-09 culture, respectively. We measured the dechlorination of TCE to ethene in time-course experiments.  Gas and Liquid Chromatography Analyses 200 mL gas samples were extracted from the headspace of bottles to measure chlorinated ethenes, ethene, and methane using a Shimadzu GC-2010 (Columbia, USA) instrument with a flame ionization detector (FID). Details on the column type and properties, GC temperature and pressure profiles, calibration curves, and detection limits for each compound measured on the GC-FID were published elsewhere [34,42,43]. H 2 was also measured from 200 mL gas samples from the headspace of the bottles using a Shimadzu GC equipped with a thermal conductivity detector (TCD). However, all samples assayed were below our GC-TCD H 2 detection limit of 0.8% vol/vol. 1 mL liquid samples were prepared for high performance liquid chromatography (HPLC; Shimadzu LC-20AT) through filtration using a 0.2 mm polyvinylidene fluoride membrane syringe filter (Pall Corporation, USA). We measured lactate, acetate, propionate, and methanol using the method outlined by Parameswaran et al. [44], except the elution total time was 40 minutes and the column temperature was constant at 50uC.

Microbial Community Analyses
We extracted genomic DNA from 0.25 g of soil or sediment using the PowerSoil DNA Isolation Kit (MO BIO Laboratories, Inc., USA) following the protocol recommended by the manufacturer. For soil/sediment-free consortia, we used pellets formed from 1.5 mL liquid culture. Before DNA extraction, we pretreated the pellets as noted in Ziv-El et al. [25]. Then, we followed the protocol for Gram-positive bacteria outlined in the DNeasy Blood and Tissue Kit (QIAGEN, USA).
We determined the relative community structure using 454 pyrosequencing in soils, sediments, and soil/sediment-free enrichment cultures. The DNA samples were analyzed at the Research and Testing Laboratory (Lubbock, TX). The targets were the combined V2 and V3 regions of the Bacterial 16S rRNA gene as formerly employed [42,45,46], using the primers 104F (59-GGCGVACGGGTGAGTAA-39) and 530R (59-CCGCNG-CNGCTGGCAC-39). Amplicon pyrosequencing was performed with 454 GS-FLX Titanium protocols [47]. We qualified raw sequences by trimming off low-quality bases and removing lowquality, chimeric sequences, and singletons by using the Quantitative Insights Into Microbial Ecology (QIIME) software package version 1.6.0 [48]. After quality control, aligning and clustering as described [49], we obtained the operating taxonomic units (OTUs) at 97% sequence similarity and assigned taxonomy to OTUs by using the Ribosomal Database Project classifier with a 50% confidence threshold [50]. We obtained the following number of qualified sequences: Cuzdrioara soil, 6,924; Carolina sediment, 7,904; and Parris Island sediment, 1,486; ZARA-10 enrichment culture, 3,141; LINA-09 enrichment culture, 2,847; and ISLA-08 enrichment culture, 2,205. The sequences reported in this paper were deposited in the Sequence Research Archive (SRA) at NCBI with the following accession numbers: SAMN02361223, SAMN02361224, SAMN02361225, SAMN02361226, SAMN02361227, and SAMN02361228.
To compare microbial diversity on an equal basis, we performed rarefaction on the OTU table by randomly selecting sequences at the same depth (1,486: the least number of sequences throughout samples) with a python script in QIIME. From the rarefied OTU table, we assessed microbial diversity within a sample (alpha diversity) through Phylogenetic Diversity (PD) using the PD Whole Tree estimator in QIIME. We also employed phylogeny-based UniFrac distance matrix [51] and generated principal coordinate analysis (PCoA) to evaluate similarity among soil and sediment samples and soil/sediment-free enrichment cultures.
We enumerated the 16S rRNA genes of Dehalococcoides mccartyi and Archaea, as well as Dehalococcoides mccartyi reductive dehalogenase genes, tceA, vcrA, and bvcA in the soil/sediment samples and the enrichment cultures via quantitative real-time PCR (qPCR). Triplicate TaqMan assays were setup for samples and standard curve points and contained the following per 10 mL reaction: 4.5 mL of 2.565 PRIME MasterMix (5 PRIME, Inc., USA), 0.5 mL F' and R' primers from 10 mM stocks, 0.03 mL probe from 100 mM stock, 0.47 mL PCR water, and 4 mL DNA template (diluted 1:10). The primers and probes were previously described for Dehalococcoides mccartyi 16S rRNA gene, tceA, and vcrA [52]; bvcA [53]; and Archaea 16S rRNA gene [54,55]. We used an Eppendorf Realplex 4S realcycler with a PCR temperature profile and cycles as outlined by Ziv-El et al. [25].

Biostimulation of Indigenous Dehalococcoides in Microcosms from Distinct Environments
We utilized uncontaminated and contaminated soil and sediments from three geographically-distinct environments to assess the biological potential for dechlorination of TCE and the subsequent enrichment of Dehalococcoides in a total of 26 microcosms. Figure 1 (left panels) shows the dechlorination profile in microcosms amended with TCE, lactate, and methanol. As depicted in Figure 1A-B (left panels), cis-DCE was the end product from TCE reduction in all microcosms set up with uncontaminated soil and sediment from Cuzdrioara and Carolina, respectively. Production of ethene from PCE reductive dechlorination was previously recorded in microcosms established with uncontaminated river sediments in Löffler et al. [56]. In the microcosms with Parris Island sediment, dechlorination proceeded beyond cis-DCE ( Figure 1C, left panel). A representative Parris Island microcosm producing VC and ethene is shown in Figure 1C (left panel). In fact, 40% of the microcosms from different core depths formed VC and ethene as early as 30 days after microcosm establishment. The dechlorination activity in our laboratory microcosms ( Figure 1C, left panel) is consistent with reports of indigenous dechlorinating activity at the Parris Island site, where TCE, cis-DCE, VC, and ethene were detected from dechlorination of PCE after biostimulation with emulsified vegetable oil [41].
We sought to understand the differences between the microcosms that could or could not be biostimulated to produce VC and ethene. qPCR data revealed that Dehalococcoides mccartyi were present in the three soil/sediment inocula. Interestingly, the 16S rRNA gene copies of Dehalococcoides mccartyi were lowest in the Parris Island sediment ( Figure S1), even though Parris Island microcosms readily dechlorinated TCE to VC and ethene ( Figure 1C, left panel). In Parris Island and Carolina inocula, Dehalococcoides mccartyi were detected in a range of 10 5 Dehalococcoides mccartyi 16S rRNA genes g 21 sediment, while in Cuzdrioara they were detected at 10 6 genes g 21 soil ( Figure S1). The amount of Dehalococcoides mccartyi gene copies contained in our samples are comparable to previously published Dehalococcoides-like Chloroflexi densities from a variety of soils and sediments [57]. Note that the characterized reductive dehalogenase genes (tceA, vcrA and bvcA) coding for enzymes involving dechlorination of TCE to VC (TceA [58]), cis-DCE and VC to ethene (VcrA [59] and BvcA [60]) were also present in the three soil/sediment inocula ( Figure S1). The presence of the characterized reductive dehalogenase genes confirmed that the microbiological potential of Dehalococcoides mccartyi was not the limitation and that overlapping function redundancy for complete reduction existed in the cis-DCE stalled microcosms and those producing ethene.
Lactate and methanol were readily fermented through acetate and propionate, and methane evolution was recorded within the first weeks of incubation in these microcosms ( Figure S2). Hence, we postulated that the electrons from the fermentable substrates were being utilized by H 2 -competing microorganisms growing on electron acceptors from the soil and sediment and on HCO 3 2 from the medium. On day 46, we transferred soil/sediment-free supernatant from each microcosm bottle stalled at cis-DCE ( Figure 1A-B, middle panels). The original Cuzdrioara and Carolina microcosms stalled at cis-DCE were biostimulated two additional times with 5 mM lactate (day 46 after the transfer and day 180) to replenish the electron donor, and were incubated for up to 200 days. It is likely that some H 2 produced during fermentation was initially available to Dehalococcoides for dechlorination of TCE to cis-DCE. However, the additional electron donor and the extended incubation did not further advance cis-DCE reduction. This indicated that Dehalococcoides able to dechlorinate past cis-DCE were outcompeted by other microorganisms, and H 2 became limiting for Dehalococcoides in the microcosms. On the other hand, methane concentrations increased throughout incubation, reaching up to 7.2 mmol L 21 and 8.8 mmol L 21 in Cuzdrioara and Carolina microcosms, respectively ( Figure S2), showing unmistakably that methanogenesis was one of the biological processes benefiting from our biostimulation efforts. Contrary to the observations from Figure 1A-B (left panels) which show only partial reduction of TCE to cis-DCE, we did achieve complete dechlorination of TCE to ethene in the soil/sediment-free mixed cultures resulting from the microcosm transfers ( Figure 1A-B, center and right panels). This confirmed that indigenous cis-DCEand VC-respiring Dehalococcoides could indeed be biostimulated, and brings supporting evidence that the dilution of the soil/sediment and donorcompeting microbial sinks facilitated this outcome.

Characterization of Soil/Sediment-Free Cultures Enriched in Dehalococcoides mccartyi
Through Figure 1A-B (left panels), our study exemplifies that microcosm data can fail to predict the ''true'' reductive dechlorination potential in an environment. In spite of this, we showed that ethene could be obtained as the reduced end product of TCE dechlorination through a specific enrichment process. We further sought to evaluate the impact of selective enrichment and culturing on the soil/sediment-free microbial communities developed from the three distinct environments. ZARA-10, LINA-09, and ISLA-08 were maintained under identical batch-fed growth conditions. The majority of the electrons from lactate and methanol were channeled to fermentation products (acetate and propionate, Figure S3). HCO 3 2 -reducing methanogens belonging to Archaea continued to be detected ( Figure S1) and active ( Figure S2) in all enrichment cultures; however, as shown in Figure S2, methane production diminished when compared to the activity in the microcosms. As for the TCE dechlorinating activity, Figure 1A-C (right panels) reveals that, as a result of the enrichment protocol, fast reduction of TCE to ethene was achieved in all three enrichment cultures, regardless of the environment where the microbial inocula originated. Moreover, the culture performance parameters tabulated in Table 2 show similarly high maximum observed transient conversion rates (on the order of mM Cl 2 released per day) in ZARA-10, LINA-09, and ISLA-08 enrichment cultures, and dechlorination of 0.5 mmol L 21 TCE to $80% ethene in as short as 1.7 days.

Insights from the Composition of Microbial Communities
To gain further insights into the differences between the microcosms that could or could not be biostimulated beyond cis-DCE, we took advantage of high throughput sequencing. The outer pie charts in Figure 2A-C illustrate the 454 pyrosequencing data at class level from Cuzdrioara soil, Carolina sediment, and Parris Island sediment. The class of interest for TCE to ethene respiration is Dehalococcoidia, containing the genera Dehalococcoides [1], Dehalogenimonas [61], and Dehalobium [62]. Dehalococcoidia was in low abundance in all soil and sediment samples (,1% of total sequences: Cuzdrioara, 0.2%; Carolina, 0.25%; Parris Island, 0.4%), and hence, is not shown in Figure 2A-C. As seen in Figure 2A-C (outer pie charts), the Cuzdrioara and Carolina environments were more diverse than the Parris Island sediment and were predominantly populated by a-, b-, c-, d-, and e-Proteobacteria. The microbial communities in Cuzdrioara soil and Carolina sediment (Figure 2A-B) concur with previously described microbial communities where sulfate [49,63], sulfur [63], iron [64], nitrate [49,65], and HCO 3 2 [42,66] were abundant. Compared to Cuzdrioara and Carolina samples, the Parris Island sediment contained fewer classes ( Figure 2C) and had a higher relative abundance of Dehalococcoidia.
While in low abundance in the soils and sediments, relative to the other bacterial classes, Dehalococcoidia became one of the most prevalent taxa in all three enrichment cultures. This is depicted in the inner pie charts in Figure 2A9-C9: ZARA-10, 9%; LINA-09, 3%; ISLA-08, 21%. Furthermore, the predominant classes in all three originating environments, a-, b-, c-, and e-Proteobacteria were absent (zero sequences) in the enrichment cultures ( Figure 2A9-C9). We believe this outcome was obtained by removing or diluting other competing electron acceptors present in the soil/sediment which were supporting microbial guilds competing for the electron donor. The most abundant sequences for all three enrichment cultures belong to Clostridia. The obvious increase in relative abundance of this class containing fermenting bacteria was the result of feeding excess fermentable substrates (lactate and methanol) throughout the enrichment process.
The family Geobacteraceae within the d-Proteobacteria, containing bacterial members known to respire TCE [67], was maintained in low abundance (,1%) in the enrichment cultures ZARA-10 and LINA-09. A different response was found for the enrichment of anode-respiring bacteria by Miceli et al., who employed the same soil and sediment samples from Cuzdrioara and Carolina in microbial electrochemical cells [64]. In the study by Miceli et al., when garden soil from Cuzdrioara was used as a microbial inoculum, the resulting enrichment was dominated by d-Proteobacteria (,90% relative abundance) [64]. When the source of microbes was Carolina mangrove sediment, ,60% of the enriched biofilm was comprised of a-, c-, and d-Proteobacteria [64]. The elimination or minimization of these Proteobacterial classes in the enrichment cultures from our study is attributed to the selective conditions provided in the medium, with TCE and HCO 3 2 as sole electron acceptors.

Diversity Analyses Reveal Convergent Enrichment Cultures
We conducted alpha diversity analyses using phylogeny-based metrics (PD index) to estimate microbial diversity. As depicted in Figure 3A-C, the PD values were low and comparable for all three enrichment cultures, indicating low microbial diversity. This was achieved from Cuzdrioara soil and the Carolina sediment ( Figure 3A-B), which had PD values approximately four fold higher than that of Parris Island ( Figure 3C). Furthermore, PCoA between samples (beta diversity) reveals that heterogeneous soil and sediment samples (blue symbols, Figure 3D) converged to very similar, highly efficient TCE-respiring microbial communities after the enrichment (green symbols, Figure 3D).

Effect of Enrichment Techniques on Growth of Dehalococcoides mccartyi
Enumeration of Dehalococcoides mccartyi was achieved through qPCR targeting the 16S rRNA genes (Table 2 and Figure 4) and their reductive dehalogenase genes, tceA, vcrA, and bvcA ( Figure 4). The three enrichment cultures exhibited some microbiological differences with respect to the Dehalococcoides mccartyi strains enriched. For example, vrcA was not detected in ISLA-08 ( Figure 3) even though it was present in the Parris Island sediment ( Figure S1). However, common to all enrichment cultures were the very high (and similar) densities of Dehalococcoides mccartyi of ,10 9 Dehalococcoides cells mL 21 (or 10 12 cells L 21 ) ( Table 2 and Figure 4). These densities were mainly the result of the enrichment protocol, where high doses of TCE (1.5-3 mmol L 21 total over three additions) were subsequently reduced by Dehalococcoides mccartyi coupling growth to dechlorination. The minimization of microbial competitors, especially hydrogenotrophic methanogens, also contributed to achieving these high Dehalococcoides densities. The high concentrations of TCE fed to Dehalococcoides may have also directly suppressed the methanogenic populations as initially reported by DiStefano et al. in enrichments fed with PCE, methanol, and yeast extract [15].
Taken together, the rates and the times required for complete reduction to ethene of the TCE supplied (Figure 1, right panels), and the resulting Dehalococcoides mccartyi concentrations (Table 2 and Figure 4) compare favorably to previously published values tabulated by Ziv-El et al. [25,68]. Dechlorination rates and Dehalococcoides mccartyi densities, two important and interconnected factors for successful bioremediation, vary sometimes by orders of magnitude [25,68] between previously characterized cultures. A contributing factor to these variances could be the enrichment technique employed, as the compared studies used different conditions and different stimulation techniques for the development and cultivation of reductively dechlorinating cultures. Our results support the idea that a robust microbial community, where Dehalococcoides thrive, can be achieved under the enrichment conditions described herein.

Bioaugmentation of Microcosms with Their Respective Enrichment Cultures
One important aspect of our study, exemplified with the Cuzdrioara soil and Carolina sediment, is the ability to stimulate the production of VC and ethene and the growth of cis-DCE and VC-respiring Dehalococcoides. To strengthen this point, we designed a simple experiment to show that, once Dehalococcoides are enriched and in high abundance, they can better compete in the complex soil or sediment environments from which they originated. For this, we re-established microcosms containing Cuzdrioara soil and Carolina sediment and bioaugmented the microcosms with a 1% vol/vol inoculum of ZARA-10 or LINA-09 enrichment culture, respectively, to more appropriately reflect the dilution factor in bioremediation scenarios at contaminated sites. As seen in Figure 5A-B and supportive of our hypothesis, with both enrichment cultures dechlorination of TCE proceeded to ethene and we achieved close to complete dechlorination of 0.25 mmol L 21 TCE to ethene in ,30 days. The rates of reductive dechlorination obtained with this smaller inoculum exclude the possibility that the cis-DCE stall resulted from components in the soil/sediment inhibiting the native Dehalococcoides.

Outlook on Bioremediation Using Dehalococcoides
For bioremediation of PCE or TCE contaminated sites, microcosm experiments have historically been utilized as indicators of indigenous microbial activity [14]. The results of microcosm experiments help researchers and bioremediation practitioners decide whether biostimulation or bioaugmentation is the appropriate treatment for decontamination of environments polluted by chlorinated solvents [14]. In cases where incomplete dechlorination was observed in microcosms, this has been attributed to the presence of inhibitors in the soil or the lack of Dehalococcoides mccartyi capable of complete dechlorination. Our findings clearly show that neither result from Cuzdrioara nor Carolina biostimulated microcosms could be explained by these two hypotheses. Instead, an electron donor competition is proposed, supported by our data, in which components of the soil or sediment serve as electron acceptor for competing H 2oxidizing microorganisms. Our results bring experimental evidence towards a new possible explanation to ''unsuccessful'' microcosm experiments. If indeed microbial competition for electron donor is a major determining factor in the success of established microcosms, it will certainly be a determining factor in bioremediation as well, and adding excess electron donor to biostimulate could prove unsuccessful. Furthermore, through the soil/sediment-free enrichment cultures developed, we bring evidence linking fast rates of TCE to ethene dechlorination and high densities of Dehalococcoides mccartyi to the selective enrichment protocol. In our study, these rates and densities were independent of the origin of the microbial inocula and the end-product of reductive dechlorination in microcosms, which bring about implications for potentially improving bioremediation in chlorinated ethene-contaminated environments.