Mapped Clone and Functional Analysis of Leaf-Color Gene Ygl7 in a Rice Hybrid (Oryza sativa L. ssp. indica)

Leaf-color is an effective marker to identify the hybridization of rice. Leaf-color related genes function in chloroplast development and the photosynthetic pigment biosynthesis of higher plants. The ygl7 (yellow-green leaf 7) is a mutant with spontaneous yellow-green leaf phenotype across the whole lifespan but with no change to its yield traits. We cloned gene Ygl7 (Os03g59640) which encodes a magnesium-chelatase ChlD protein. Expression of ygl7 turns green-leaves to yellow, whereas RNAi-mediated silence of Ygl7 causes a lethal phenotype of the transgenic plants. This indicates the importance of the gene for rice plant. On the other hand, it corroborates that ygl7 is a non-null mutants. The content of photosynthetic pigment is lower in Ygl7 than the wild type, but its light efficiency was comparatively high. All these results indicated that the mutational YGL7 protein does not cause a complete loss of original function but instead acts as a new protein performing a new function. This new function partially includes its preceding function and possesses an additional feature to promote photosynthesis. Chl1, Ygl98, and Ygl3 are three alleles of the OsChlD gene that have been documented previously. However, mutational sites of OsChlD mutant gene and their encoded protein products were different in the three mutants. The three mutants have suppressed grain output. In our experiment, plant materials of three mutants (ygl7, chl1, and ygl98) all exhibited mutational leaf-color during the whole growth period. This result was somewhat different from previous studies. We used ygl7 as female crossed with chl1 and ygl98, respectively. Both the F1 and F2 generation display yellow-green leaf phenotype with their chlorophyll and carotenoid content falling between the values of their parents. Moreover, we noted an important phenomenon: ygl7-NIL's leaf-color is yellow, not yellowy-green, and this is also true of all back-crossed offspring with ygl7.


Introduction
Genetic purity of crop plants, which has a critical impact on increasing crop yields, is gradually becoming the focus in hybrid rice production [1]. Molecular markers can be used to rapidly identify seed purity yet are not useful for identifying off-type plants. Plants with a distinct color phenotype can be easily identified and removed, so leaf-color has become a suitable marker for maintaining the genetic purity of hybrid rice [2]. In addition, study of the genetic mechanism of leaf-color mutations would further our understanding of chlorophyll biosynthesis and degradation, chloroplast development, tetrapyrrole synthesis, and photosynthesis.
Mg-chelatase, which was first discovered in photosynthetic bacteria, is the first enzyme catalyzing the committed step of chlorophyll synthesis. Mg-chelatase consists of three subunits: I (40-kDa), D (70-kDa), and H (140-kDa) [33]. Only one copy of all the three subunits exists in rice. However, there is more than one subunit I in some species, such as Arabidopsis [34]. Mg-chelatase activity can only be detected when all three subunits are present [35]. Subunit I of Mg-chelatase belongs to the functionally diverse superfamily of AAA proteins and has the ATPase activity which is associated with various cellular activities [36]. Subunit D of Mgchelatase has three consecutive regions: an N-terminal domain homologous to the AAA module of subunit I but without ATPase activity, a central acidic and Pro-rich region, and an integrin I domain with an unknown function at the C-terminus [37]. ATP combines with subunit I, subunit D, and Mg 2+ to form the I-D-Mg-ATP complex. Subunit H combines with Protoporphyrin IX to form the H-Protoporphyrin IX complex. Then, I-D-Mg-ATP and H-protoporphyrin IX are combined together to hydrolyze ATP. Simultaneously, Mg 2+ is added to Protoporphyrin IX to form Mg-Protoporphyrin IX [38].
Using the map-based cloning method and T-DNA insertion mutants, the five genes Chl1 [39], Chl9 [39], Ygl9 [40], Ygl3 [9], and ChlH [41] have been cloned in rice. Chl1, Ygl98, and Ygl3 code for OsChlD, and they are alleles. Chl9 codes for OsChlI and ChlH codes for OsChlH. Plants with ch11 exhibit yellowish-green leaves at the seedling stage, and then the color turns to a normal green at maturity. The mutational allele of Chl1 has a single base change from G to A at position 1176 bp which is located at the 8 th exon. This nucleotide change leads to one aa conversion from Arg (R) to Gln (Q) at position 393 aa [39]. The ygl98 mutant exhibits a yellow-green leaf phenotype throughout the growing season and lower grain output. Mutational Yg198 has a single base change from G to A at position 1522 bp which is located at the 10 th exon. This conversion results in an amino acid change from Ala (A) to Thr (T) at position 508 aa [40]. The ygl3 mutant displays a yellowy-green leaf-color throughout the growth cycle, lower plant height, and lower grain yield. The mutational Yg13 has a single base change from G to C at position 1009 bp which is located at 6 th exon. This mutation leads to an amino acid change from Ala (A) to Thr (T) at position 337 aa [9].
The rice variety An Nong Biao 810S is a natural, yellowy-green leaf mutant selected from the PTGMS (photo-and thermosensitive genetic male sterile) rice line An Nong 810S maintained by Huaihua Vocational and Technical College of Hunan [42]. This plant leaf displays a permanent yellowy-green color throughout its life. Several studies reported no remarkable differences between wild type and the mutant in other primary agronomic traits [43,44]. Compared to the wild type An Nong 810S, the mutant has a sharp drop in chlorophyll content. This variety as a visual marker is useful for testing the varietal purity of hybrid rice seeds. The leaf-color phenotype in this mutant is controlled by the single recessive nuclear gene Ygl7 which encodes a magnesium-chelatase ChlD. In this study, a single-nucleotide mutation from T to C at position 1883 bp of gene Ygl7 which leads to a change in the protein (Leu to Ser at position 628 aa)is identified by genetic sequencing. The RNAi assay of normal Ygl7 has been conducted in Nipponbare. Interestingly, back-crosses between the mutant ygl7 and other varieties displayed yellowleaves. This research is significant for the application of a leaf-color marker gene in hybrid rice production and for exploration of the chlorophyll synthesis mechanism.

Plant materials
These experiments were conducted at Hunan Changsha and Hainan Sanya in April 2009 to October 2013. We used the following varieties.
The mutant ygl7 was a spontaneous yellow-green leaf mutant derived from multiplication of the PTGMS rice variety An Nong 810S (810S) (Oryza sativa L. ssp. indica) [42]. For genetic analysis, we used three combinations derived from crossing ygl7 with two indica cultivars (353 and 9311) and a japonica variety termed Nipponbare (NPB). The F 2 population derived from crossing ygl7 with NPB was used to map Ygl7. The ygl7's near-isogenic line ygl7-NIL was used as a genetic complement. We constructed the nearisogenic line ygl7-NIL to function as acceptor material of the genetic transformation. Nipponbare was the acceptor parent backcrossed with the donor parent, ygl7. The yellow plants' population from BC 4 F 2 is ygl7-NIL. The Chl1 mutant (obtained from Professor Nam-Chon Paek, College of Agriculture and Life Sciences, Seoul National University) and the ygl98 mutant (obtained from Professor Wang Ping-rong, Sichuan Agriculture University) are the OsChlD allele's mutant. All plant materials were planted under a standard management.

Genetic analysis and mapping
The leaf-color phenotype in the F 1 and F 2 populations were designated as the wild type (normal green leaf) and the yellowygreen leaf phenotype was considered the mutant. The F 2 segregation ratios were analyzed with a x 2 goodness of fit test using the Excel software.
DNA was extracted from leaves using the improved CTAB method [45]. A total of 1199 molecular markers were used in this study, including 1130 SSRs (simple sequence repeat), 67 SFPs (single feature polymorphism), and 2 STSs (sequence tagged site). The SSR markers were obtained from Gramene (http://www. gramene.org/microsat/). The SFP and STS markers were designed using the Primer 5.0 software (Table S2). First, we used 1100 SSRs and 67 SFPs to select polymorphic markers from Ygl7 and NPB. Then, we used the entire gene pool to obtain potential linked markers. We used those markers in primary mapping of F 2 individuals with the yellowy-green leaf phenotype that were selected from the cross of Ygl7 and NPB. To fine map the Ygl7 locus, we selected an additional 30 SSRs, 4 SNPs, and 2 STSs. Six molecular markers were used to screen recombination events from 2,849 F 2 individuals for fine-mapping. Linkage analysis was conducted with MAPMAKER3.0, and the linkage map was constructed with MapDraw V2.1.

Sequence analysis
Within the fine mapped chromosome region, candidate genes were screened according to the annotation database of the NCBI. Specific primers were designed according to the genome sequences of NPB. These primers were used to amplify the candidate genes from the ygl7 mutant and its wild type parent 810S. The amplified products from DNA and RNA were sequenced and structurally analyzed to determine the target gene and the mutation site within the target gene. Total RNA was extracted using the TRIZOL method [46].

Genetic complementation and RNAi suppression of YGL7
The PCR products of a full-length YGL7 cDNA (primer D-18, Table S2) were digested with BamHI and KpnI and then inserted into the vector pLYL18, which was derived from pCAMBIA1300 using an ubiquitin promoter ( Figure S1). Because callus of the ygl7 mutant (an indica variety) was difficult to obtain, the recombinant plasmid designated as pLYL18-YGL7 was introduced into Agrobacterium tumefaciens EHA105. It was then used to infect calli of individuals with the homozygous ygl7 allele in japonica genetic background selected from one NPB/ygl7 BC 4 F 2 population (ygl7-NIL). Transformation was conducted according to a published protocol [47].
The pFGC5941 with a 35S promoter and a petunia CHSA (chalconesynthase A) intron was used as an RNAi vector. The PCR product is 592 bp conserved segments of cDNA YGL7 (primers DXJRNAi II, Table S2). The sense segment link to pFGC5941 was accomplished using NcoI and AscI, and BamHI and XbaI were used to link the anti-sense segment ( Figure S1), which was then transformed into NPB as described above.

Gene Expression Analysis
For quantitative real-time PCR (qRT-PCR) analysis, total RNA was extracted from young leaves of ygl7, 810S and ygl7-NIL using an RNA Prep Pure Plant kit (Tiangen Co., Beijing, China). RNA was reverse transcribed using a SuperScript II kit (TaKaRa). Realtime PCR was performed using a SYBR Premix Ex Taq TM kit (TaKaRa) on an ABI prism 7900 Real-Time PCR System. We selected two types of genes for analysis. The first type are associated with chlorophyll biosynthesis and includes ChlD (YGL7), ChlI, ChlH, YGL1, HEMA1, and PoPA [32]. The second type are associated with photosynthesis, including the genes Cab1R, Cab2R, PsaA, PsbA, and RbcL [32]. qRT-PCR primers come from Zhou KN's paper [32] (primers were DXJCHLD to DXJrbcL, Table S2).

Chlorophyll fluorescence and photosynthetic pigments
Chlorophyll fluorescence was determined through IMAGING-PAM at the early heading stage. Fo, Fv'/Fm', ETR, qN, and qP were used in our study.
The chlorophyll (Chl) and carotenoid (Car) contents were measured using a spectrophotometer according to the method described by Tang Yan-lin [48]. To summarize briefly, equal weights of freshly collected second top leaves from the heading stage were immersed in extracting solution for 12 h under dark conditions. The extraction solution had a volume ratio of acetone, ethanol, and distilled water of 4.5: 4.5: 1. Residual plant debris was removed by centrifugation. The supernatants were analyzed with a DU 1700 UV/Vis Spectrophotometer at 440 nm, 663 nm, and 645 nm using the following equations: Chla

Characterization of the ygl7 mutant and ygl7-NIL
The rice mutant ygl7 has a yellowy-green leaf under paddy field conditions; ygl7 exhibited a distinct and steady phenotype throughout its lifespan when grown in Hunan Province and Hainan Province from 2009 to 2013. The ygl7 plants showed an enduring yellow-green leaf phenotype that began when the first leaf completely unfolded (Figure 1 A, B). This color extended to the spikes, stalks, and other above-ground plant parts. Compared to 810S, there were no significant differences in main agronomic traits including the growth period, plant height, spike length, the spike number per plant, the grain number per spike, effective tiller, maturing rate, and 1000-grain weight (Table 1). Compared to same combinations which crossed with 810S, there were no significant differences in main agronomic traits, including the growth period, maturing rate, and 1000-grain weight ( Table 2). These results suggested that this phenotype does not influence grain output and hybrid characteristics. The contents of Chl a, Chl b, and carotenoids were significantly reduced in ygl7 by about 50% compared to the wild-type parent 810S (Figure 1 C). These results suggested that the ygl7 mutant phenotype mainly resulted from reduced contents of photosynthetic pigments. In addition, the Chl a/b ratio of ygl7 increased compared to wild type, which indicated that the drop of Chl b was greater than Chl a in the ygl7 mutant.
The ygl7-NIL is the ygl7's near isogenic line. To construct ygl7-NIL, the donor parent ygl7 was back-crossed with the receptor parent NPB. We found that ygl7-NIL displayed an abnormal leafcolor phenotype. The leaf-color of ygl7-NIL is yellow instead of yellow-green (Figure 1 D). The contents of Chl a, Chl b, and carotenoids were reduced in ygl7-NIL compared with ygl7 (Figure 1  E). The same phenomenon was observed when ygl7 was backcrossed with indica rice (data not shown). The situation is probably caused by an altered genetic background. It is also probably caused by the mutation of how YGL7 joins in other regulatory pathways. This phenotypic characterization improves the ability to cultivate better sterile lines and will facilitate hybrid seed production and purification for the growth of rice crops.

Single amino acid change in Ygl7
All F 1 plants of the ygl7 mutant crossed with normal green rice varieties displayed normal green leaves. The F 2 populations from ygl7/9311, ygl7/353, and ygl7/NPB showed a segregation ratio of 3:1 (green: yellow-green plants, x 2 ,x 2 0.05 = 3.84, P.0.05; Table 3). These data indicated that the yellow-green leaf phenotype in the ygl7 mutant was controlled by a single recessive nuclear gene.
Locus mapping of the Ygl7 gene was performed using the F 2 population from a ygl76NPB mutant cross. A Bulked Segregant Analysis (BSA) suggested that the Ygl7 locus was possibly located on chromosome 3 or 12 (Table S3). The Ygl7 locus was initially mapped to within 3.9 cM between RM1308 and SFP-3-6 on the long arm of chromosome 3 based on 368 typical yellow-green leaf F 2 individuals (Figure 2 A). With a total of 651 F 2 homozygous mutant plants, we fine-mapped the Ygl7 locus to a 64.8 kb interval between SSR marker RM16106 and STS marker STS3-1 on BAC clones AC135595 and AC137507 (Figure 2 B, C). We found 12 ORFs were predicted within this region (Figure 2 D, Table S4). Among these ORFs, LOC_Os03g59640 was highly correlated with the ygl7 phenotype. The LOC_Os03g59640 ORF encodes the subunit CHLD of magnesium-chelatase. Comparison of sequences indicated that the single base change (T1883C) located in the 12 th exon of Os03g59640 resulted in a missense mutation (L628S) in the encoded product (Figure 2 E, F). The cDNA sequenced from Os03g59640's was the same missense mutation as the sequenced DNA. For these reasons, this gene was identified as the candidate gene of Ygl7 that caused the phenotype of yellowgreen leaves during the whole growth stage.

Confirmation of Ygl7 function
To confirm whether the single base change in Ygl7 is responsible for the mutant phenotype, we performed a complementation analysis. Complementation analysis was performed by transforming ygl7-NIL with the YGL-cDNA transgene in pLYL18. The plasmids with YGL7 contained the bacterial hygromycin B phosphotransferase (HptII) gene as a selection marker (Figure 3  B). The positive transgenic plants were almost reverted to green leaf and their Chls and Cars contents approached the levels of wild type plants (Figure 3 A, C).
RNA interference was conducted by transforming NPB with a 594 bp conservative segment of cDNA transgene in pFGC5941. A total of 9 independent transgenic lines were obtained. The first three leaves of RNAi transformed plants were green, but over time all leaves gradually turned from pale-green (Figure 4 A, B) to yellowy-green, then to yellow, then to almost white, and then died (with about seven-to-eight leaves) (Figure 4 D, E). This data suggested that RNAi-mediated silencing of YGL7 causes a lethal phenotype of in the transgenic plants. Real-time PCR analysis showed that the magnitude of YGL7 expression in the transgenic lines was reduced compared to the NPB (Figure 4 C, F).
These results demonstrate that Os03g59640 corresponds to the Ygl7 gene. Moreover, silencing Ygl7 is a gradual process. Once the silencing is completely, rice is unable to synthesize chlorophyll which leads to the death of the plant. The combination of RNAi and complementation analysis shows that the mutation of YGL7 does not cause a loss of OsChlD's functions. The mutation instead endows the protein with a new function.

The ygl7 mutant uses light energy efficiently
Real-time PCR was performed to study the expression profile of ygl7. In ygl7, ChlD, ChlI, and ChlH (Mg-chelatase D, I and H subunit) are up-regulated compared in the ygl7 to the WT (810S). However, ChlD was much lower in ygl7 than ChlI and ChlH ( Figure 5). This illustrates that the mutation ChlD led to an accumulation of the three subunits of Mg chelatase, especially ChlI and ChlH. This shows that the yellow-green leaf resulted from unusual chlorophyll biosynthesis controlled by the accumulation of ChlD, ChlI, and ChlH.
Real-time PCR was performed to determine whether the Ygl7 gene has any relationship with other genes and to determine what kind of genes are related to Ygl7. Those genes associated with chlorophyll biosynthesis (including ChlD, ChlI, and ChlH), Hema1 (glutamyl tRNA reductase), and PoPA (NADPH-dependent protochlorophyllide oxidoreductase) were up-regulated in ygl7 compared with wild type. Similarly, those genes associated with photosynthesis, including Cab1R (light-harvesting Chl a/b-binding proteins of PSII), PsaA (reaction center polypeptides of PSI), PsbA (reaction center polypeptides of PSII), and RbcL (the large subunit of Rubisco), were also mostly up-regulated in ygl7. However, Ygl1 (Chl synthetase) and Cab2R (light-harvesting Chl a/b-binding proteins of PSII) were down-regulated in the ygl7 mutant ( Figure 5). Thus, it seems that the ygl7 mutant affects transcription of not only the Ygl7 gene itself but also of genes associated with Chl biosynthesis and photosynthesis. The normal accumulation of  ChlD might attract accumulation of other Chl biosynthesis-related genes that then destroys the chlorophyll biosynthetic pathway. Interestingly, expression of these genes in Ygl7-NIL was at almost normal levels ( Figure 5). Only a few Chl biosynthesisrelated genes and photosynthesis-related genes were affected by Ygl7. We have no plausible explanation for this at present.
We performed Chlorophyll Fluorescence in ygl7, chl1, and ygl98. The Fo (minimal fluorescence) values from this chlorophyll fluorescence were consistent with the leaf chlorophyll content in ygl7, chl, and ygl98 ( Figure 6). The data illustrated that the chlorophyll fluorescence detector is trustworthy. Fv'/Fm' (photo-chemical efficiency of PSII in the light), ETR (photosynthetic electron transport rate), and qP (photochemical quenching coefficient) were higher in ygl7 than WT, chl1, and ygl3 ( Figure 6). This indicated that conversion efficiency of light energy and capture efficiency of solar energy in the PSII center is higher in ygl7. The transport number of photosynthetic quantum yield and the transport rate of acyclic electron from antenna pigments in PSII were also higher in ygl7. qN is a coefficient of nonphotochemical quenching. It indicated that the absorbing light energy from antenna pigments in PS II is emitted as heat instead of being used for photosynthetic electron transport. When antenna    Leaf-Color Gene Ygl7 in a Rice PLOS ONE | www.plosone.org pigment from PSII absorbs excess light energy, the inactivation and disruption of the photosynthetic apparatus will happen if not timely dissipated. So qN is a self-protection mechanism. There is no sharp difference in qN between ygl7 and WT ( Figure 6). This demonstrates that ygl7 could protect the photosynthetic apparatus to a certain extent. The ygl98 and chl1 have not sufficient qN function. In general, the three leaf-color mutant ygl7 has high conversion efficiency of light energy, capture efficiency of solar energy, and protection of photosynthetic apparatus. The ygl7 is the only phenotype without influence upon grain output amongst the three mutants. Genes associated with photosynthesis were mostly up-regulated in ygl7. Ygl7 possessed a higher photosynthetic efficiency in earlier physiological and biochemical studies [43]. All this data suggests that photosynthesis-related genes might be up-regulated by the accumulation of ChlD. The mutation of YGL7 gives the protein a new function that can put up photosynthesisrelated genes.
Differences among ygl7, chl1, ygl3 and ygl98 mutants We planted the three mutants, ygl7, chl1 and ygl3, at the field station under a standard management. In order to verify whether their mutations represent alleles, ygl7 as the female parent was crossed with chl1 and ygl98, respectively. Of the three mutants we planted under field conditions, all F 1 and F 2 plants had yellowygreen leaves (Figure 7, A). The content of chlorophyll and carotenoid in the F 1 and F 2 plants decreased to some extent (Figure 7, B). This established that the three mutants' genes are alleles. The Chl1's leaf-color was the most yellow of the three mutants, while ygl7 and ygl98 had a yellow-green leaf-color. However, there is some discrepancy in chl1's phenotype between populations in China and Korea. In our experiment, chl1 plants had sharp yellow leaves (Hunan and Hainan Provinces). In a prior study, the chl1 mutant displayed a yellowish-green leaf phenotype only at the seedling stage, and the abnormal leaf-color was first observed on the leaves of 2-to 3-week-old seedlings [39]. This difference will need further assessment in order to clearly understand the underlying causes.

Discussion
Plants with the ygl7 mutant maintained a yellowy-green leafcolor throughout their growth cycle, and had a sharp drop in chlorophyll content compared to WT (Figure 1). Previous studies showed that photoelectron utilization rate and photosynthetic rate are higher in ygl7 compared with WT [43,49]. We also found that genes associated with photosynthesis were mostly up-regulated ( Figure 4; Figure 6). The net photosynthesis and dark respiration rates of ygl7 were higher than WT [33], and the yellow-green phonotype did not negatively impact agronomic traits (Table 1). Thus, our analyses show that the ygl7 mutant is also highly efficient in its ability to use light energy ( Figure 5, 6). In addition, seeds of hybrids crossed with ygl7 had the same grain output as the WT (Table 2). Thus, this mutant ygl7 is an ideal leaf-color marker. Interestingly, the leaf-color of ygl7's hybrids was significantly more yellow than ygl7 itself (Figure 1). New male sterile lines show a yellow-leaf-color when the Ygl7 gene is back-crossed to any male sterile with no associated negative agronomic traits (data not shown).
Overall, our genetic analyses of the ygl7 mutant showed that it was controlled by a base mutation of the magnesium chelatase subunit D. Mg-chelatase plays a decisive role in the synthesis of chlorophyll because it catalyzes the insertion of magnesium into Protoporphyrin IX [50]. This enzyme consists of the three subunits I, D, and H. The three subunits take part in the synthesis of chlorophyll via a complex substance and also act independently in other metabolic pathways. The H subunit plays a role in reverse signaling to the nucleus within the chloroplast [51], controlling the SigntaE factor in cyanobacteria [52], and acts in the ABA signal transduction pathway [53]. The I subunit is the target protein of thioredoxin. The D subunit acts in exhibiting the gun phenotype in Arabidopsis [54]. OsChlD has four alleles: Chl1 [39], Ygl98 [40], Ygl3 [9], and our gene Ygl7. The four alleles exhibit different phenotypes in the varieties in which they are found. Based on all of this evidence we can make several inferences. First, we surmise that different mutated proteins resulted in different leaf pheno-  types. Second, we suggest that the D subunit may also play a role in other metabolic routes. However, these differences in leaf phenotype may simply be caused by variance in genetic backgrounds among the mutants. Of course, they may also be due to many other biological or ecological factors. A remaining question is what will happen to leaf-color when plants are grown with the same genetic background. Further study will be required to tease apart these different potential explanations.
More specific alleles continue to be identified with the continued study of rice genetics. At present, 14 genes have been shown to have alleles, amid the total of 53 cloned leaf-color genes. There has been little in-depth research on alleles, and this will be an ongoing focus of our research group. We crossed female ygl7 with chl1 and ygl98 mutants. They all expressed mutated leaf-color throughout their development. However, chl1 displayed an extreme yellowishgreen leaf phenotype only at the seedling stage in Korea [39]. In China, however, chl1 had a yellow leaf-color, while ygl7 and ygl98 were yellowy-green. In our study, all F 1 and F 2 plants had the yellowy-green leaf, and the chlorophyll and carotenoid contents of leaves taken from F 1 and F 2 individuals fell between the values of their two parents. Heterozygotes exhibiting an intermediate phenotypeis what is a perplexing phenomenon. It may be due to genetic neutralization, interaction of the two mutated proteins, different genetic backgrounds, or some other mechanism. Further study will address these different possibilities.
The ygl7-NIL is the form that represents the ygl7 mutant's near isogenic lines. ygl7-NIL has the yellow leaf type instead of the yellowy-green leaf of ygl7 (Figure 1). The same outcome was observed in offspring crossed with ygl7. This striking result has not been documented in previous studies of rice-leaf-color mutations. The situation is probably caused by multiple copies of the mutant gene or perhaps by an altered genetic background. We speculate that Ygl7's magnesium chelatase function may be doubled by an amino acid mutation. It rapidly synthesizes magnesium protoporphyrin, but later steps in chlorophyll biosynthesis occur at normal speed thereby leading to an accumulation of magnesium protoporphyrin. Chloroplasts may send signals to the cell nucleus to regulate photosynthesis-related genes and improve the transfer rate of photoelectrons. However, feedback regulation occurs in the latter steps of chlorophyll synthesis so this may result in abnormal chlorophyll synthesis.
The fading leaf color and ultimate death of the leaves of RNAitransformed plants (Figure 3) illustrates that rice can no longer synthesize chlorophyll once Ygl7 is completely silenced. Moreover, photosynthesis-related genes were mostly up-regulated in Ygl7 (Figure 4). These phenomena show that the mutational YGL7 protein has part of OsChlD's function and promotes photosynthesis. The YGL7 protein does not lose the function of OsChlD, but as a new protein possesses a new function. There has been some research showing that accumulation of magnesium protoporphyrin can lead to reverse signal transduction and influence photoelectron transfer via regulated photosynthesis genes [55,56].
Further research will be required to confirm that Ygl7 plays a role in other metabolic pathways and to explore what those pathways and functions might be.
In conclusion, we have conducted a study on a yellowy-green leaf mutant of rice termed An Nong Biao 810S. This mutation was controlled by the mutational gene Ygl7 which encodes for a magnesium-chelatase ChlD. The mutational ygl7 had a singlenucleotide change from T to C at position 1883 bp which was located at the 12 th exon. The base conversion led to a change of AA (Leu 628 Ser). The mutational YGL7 protein acts with partial of OsChlD's function, and it could promote photosynthesis. The phenotype is without influence upon grain output and hybrid characteristics. The ygl7 has a high light use efficiency, and the hybrid offspring from crosses between yg17 and some other varieties exhibited a yellow-leaf. All these results will improve the ability to cultivate better sterile lines, facilitate hybrid seed production, and promote the purification of rice crops.