A Protoberberine Derivative HWY336 Selectively Inhibits MKK4 and MKK7 in Mammalian Cells: The Importance of Activation Loop on Selectivity

A protoberberine derivative library was used to search for selective inhibitors against kinases of the mitogen-activated protein kinase (MAPK) cascades in mammalian cells. Among kinases in mammalian MAPK pathways, we identified a compound (HWY336) that selectively inhibits kinase activity of mitogen-activated protein kinase kinase 4 and 7 (MKK4 and MKK7). The IC50 of HWY336 was 6 µM for MKK4 and 10 µM for MKK7 in vitro. HWY336 bound to both kinases reversibly via noncovalent interactions, and inhibited their activity by interfering with access of a protein substrate to its binding site. The binding affinity of HWY336 to MKK4 was measured by surface plasmon resonance to determine a dissociation constant (Kd) of 3.2 µM. When mammalian cells were treated with HWY336, MKK4 and MKK7 were selectively inhibited, resulting in inhibition of c-Jun NH2-terminal protein kinases in vivo. The structural model of HWY336 bound to either MKK4 or MKK7 predicted that HWY336 was docked to the activation loop, which is adjacent to the substrate binding site. This model suggested the importance of the activation loop of MKKs in HWY336 selectivity. We verified this model by mutating three critical residues within this loop of MKK4 to the corresponding residues in MKK3. The mutant MKK4 displayed similar kinase activity as wild-type kinase, but its activity was not inhibited by HWY336 compared to wild-type MKK4. We propose that the specific association of HWY336 to the activation loop of MKK4/MKK7 is responsible for its selective inhibition.

Protoberberine alkaloids have anti-tumor activity in mice and cytotoxic activities against human cancer cell lines [11,12].In this study, we screened these compounds against kinases in the MAPK cascades using a protoberberine derivative library, and identified that a protoberberine derivative, HWY336, which selectively interacts and blocks MKK4/MKK7.Molecular modeling studies suggest that binding of HWY336 to the activation loop of MKK4 and MKK7 plays a major role in the inhibitory mechanism of this compound.

Kinase inhibitors
Each of 80 protoberberine derivatives was dissolved in DMSO to a final concentration of 1 mg/ml.A stock solution containing 1 mg/ml of SP600125, SB203580, or U0126 (A.G.Scientific Inc.) was also prepared in DMSO.

High salt wash and competition assays
To determine whether HWY336 binding to MKK4 and MKK7 was reversible, each purified kinase was treated with HWY336 and washed three times with kinase buffer containing varying concentrations of NaCl from 0 to 500 mM prior to the kinase assay.Purified MKK4 and MKK7 were pre-incubated with 1 mg/ml MBP or 1 mM ATP prior to the addition of HWY336 to determine whether HWY336 competed with protein substrate or ATP for binding to MKK4 and MKK7.

Western blot analysis
After HWY336 treatments, total protein (50 mg) of cell lysate was separated by 10% SDS-PAGE and transferred to a PVDF membrane.Specific proteins were detected using the following primary antibodies: anti-JNK, anti-p-JNKs, anti-p38, anti-p-p38, anti-MKK4, and anti-p-MKK4 (Cell Signaling Technology, Inc.).

Surface plasmon resonance (SPR) measurement of the interaction between HWY336 and MKK4
The optical set-up for SPR detection consisted of two concentric dual motorized stages that were employed to implement anglescanning capability.To measure binding constants, imaging SPR detection was performed by maintaining the angle of light incidence at 59u.Light source from a He-Ne laser (36 mW, l = 632.8nm, nominal beam diameter = 650 mm, Melles-Griot, Carlsbad, CA) was p-polarized before incident on a SPR detection sample that was index-matched to an SF10 prism substrate.A p-in photodiode (818-UV, Newport) assessed the signal, which was subsequently fed to a low noise lock-in amplifier.The whole procedure was computer controlled and fully automated.Each measurement was repeated multiple times for statistical analysis.
SPR sample chips were fabricated by evaporating a 2 nm thick chromium adhesion layer and a 40 nm thick gold film on an SF10 cover glass substrate.Evaporated sample chips were cleaned in a plasma cleaner (Harrick Scientific Products, Pleasantville, New York).For SPR measurements, streptavidin was first coated on the gold surface by soaking the sample in a 1 mM solution of 8-amino-1-octanethiol (AOT, Dojindo) for 8 hrs in a dark environment and incubating the gold chip with 4 mM SATP solution for 1.5 hrs in a humid chamber.SPR sample surface was deacetylated in an incubation process using de-acetylation buffer (0.5 M hydroxylamine hydrochloride, 25 mM EDTA in PBS, pH 7.4) for 25 min, followed by streptavidin attachment on the surface using 20 ml of streptavidin-maleimide solution incubated on the SPR samples for 1 hr.After PBS washing, 10 nM biotinylated MKK4 solution (Biaffin GmbH & Co. KG, Kassel, Germany) was immobilized for 2 hrs.For binding of MKK4 with HWY336, HWY336 solution with PBS buffer was injected onto the SPR samples through a fluidic channel for 120 min using a micropump (KD Scientific).

Homology modeling and molecular docking
Homology modeling for MKK4 was performed using the Modeller9v4 software that calculated a model composed of nonhydrogen atoms, based on aligning the sequence to be modeled with known related structures.A three-dimensional model was obtained by optimization of molecular probability density function using a variable target function procedure in Cartesian space that employed conjugate gradients and molecular dynamics with simulated annealing.
Molecular docking used a reported MKK7 structure and our homology model of MKK4 using the SYBYL 8.1 software package (Tripos, Inc., St. Louis, MO).Protein structure was prepared using a biopolymer module to prepare a structure that was suitable for docking assessments.Hydrogen atoms were added to the structure, atom types and charges were assigned using AMBER7 FF99, and side chain amides were modified.HWY336 was sketched using the SYBYL 8.1 sketch program and minimized using the Tripos force field and Powell method with a termination gradient set to 0.05 kcal/mol.The molecule was fully minimized with Gasteiger-Hu ¨ckel charges.The docking study was performed using the Surflex-Dock module of SYBYL 8.1, which used an empirical scoring function to score ligand and protomol guided docking [13,14].

HWY336, a protoberberine derivative, was identified as a selective inhibitor of MKK4 and MKK7
A small-scale chemical library was generated by extensively modifying protoberberine (Figure 1A).To search for selective inhibitors of kinases in MAPK pathways using the library of 80 protoberberine derivatives, in vitro kinase assays were performed with MEK1, MEK2, MKK4, MKK7, MKK3, MKK6, and MAPKs (i.e., JNKs, p38, and ERKs) immunoprecipitated from either HEK293 or CHO cells, using MBP as a common substrate for kinases (Figure 1B).In this screen, we identified HWY336 as a selective inhibitor of MKK4 and MKK7 kinase activities (Figure S1 in File S1).The chemical structure of HWY336 and its synthesis from berberine chloride, the lead compound, are shown in Figure 1C.
As shown in Figure S1A, MKK4 and MKK7 kinase activity towards MBP was blocked completely by incubation with HWY336, while treatment with berberine chloride or another protoberberine derivative (HWY289) had no effect (Figure S1 in File S1).This selective inhibitory effect of HWY336 was observed regardless of the cell type from which MKK4 or MKK7 was purified (Figure S1A in File S1).Moreover, HWY336 inhibition of MKK4/MKK7 was comparable to that of the well-characterized MKK1/2 inhibitor U0126, which also blocks MKK4 and MKK7 at a higher concentration of 3 mM (Figure S1A in File S1).

HWY336 selectively inhibits MKK4 and MKK7 in vitro and in vivo
The discovery of HWY336-mediated inhibition of MKK4 and MKK7 from screening led us to identify HWY336 as a specific inhibitor of MKK4 and MKK7.However, MBP used as a substrate in the screen was not a physiological target for MKK4 and MKK7.Thus, we confirmed the selective inhibition of MKK4 and MKK7 by HWY336 with a physiological substrate, JNK.MKK4 kinase activity was completely inhibited by 10 mM HWY336, and MKK7 kinase activity was blocked by 20 mM HWY336.The IC 50 of HWY336 was 6 mM for MKK4 (Figure 2A), and 10 mM for MKK7 (Figure 2B) in vitro.These data demonstrated that HWY336 inhibits MKK4/MKK7 kinase activity and blocks JNK phosphorylation.
To evaluate the selective inhibitory effect of HWY336 on the MAPK signaling pathway in vivo, we characterized the phosphorylation of endogenous JNKs and p38 by western blot analysis.Hyperosmotic stress triggers the activation of JNKs and/or p38 through their MAPK cascades in mammalian cells.We examined the effect of HWY336 on cells treated with D-sorbitol to activate JNK and p38 MAPK pathways in HEK293T cells.As shown in Figure 2C, treatment with 600 mM sorbitol led to the activation of JNKs and p38.A time course analysis revealed that JNK phosphorylation was decreased or disappeared while the amount of JNKs was not changed, when HEK293T cells were incubated in the presence of 12 mM HWY336 for 4 hrs (Figure 2C).When the sorbitol-treated cells were incubated with increasing concentrations of HWY336 for 3 hrs, phosphorylation of JNKs was reduced at 9 mM and disappeared at 12 mM of HWY336, but p38 phosphorylation was not affected (Figure 2D).Because MKK4/ MKK7 becomes activated by phosphorylation, we also tested whether HWY336 inhibits the phosphorylation of MKK4 by examining the phosphorylation of MKK4 with anti-phospho MKK4 antibody.MKK4 phosphorylation is decreased when cells were treated with increasing concentrations of HWY336 (Figure 2D), demonstrating that HWY336 also blocks the phosphorylation of MKK4.These in vivo observations are consistent with the results of in vitro kinase assays in Figure 2A and B. Together, these results strongly suggest that HWY336 blocks the activation of JNKs by inhibiting upstream MKK4 and MKK7 both in vitro and in vivo.

HWY336 directly binds to MKK4
When purified MKK4 or MKK7 was incubated with HWY336 and washed with kinase buffer containing varying concentrations of NaCl prior to the kinase assay, MKK4 and MKK7 activity was restored with increasing NaCl concentration (Figure S2A and S3A in File S1).These results suggested that the interaction of HWY336 to MKK4 and MKK7 was reversible and likely involved noncovalent interactions.
To verify the binding affinity, we measured the interaction of MKK4 and HWY336 by SPR spectroscopy in vitro.SPR measurement was performed by injecting HWY336 through a microfluidic cell with varying concentrations from 100 nM to 1 mM.The concentration of MKK4 was fixed at 10 nM during immobilization onto the SPR sample surface.Figure 3 shows the relative amount of HWY336-MKK4 complex over time as a result of the interaction between HWY336 and MKK4.The relative amount of HWY336-MKK4 complex was assumed to be proportional to changes of light reflectance observed in SPR spectroscopy and defined here as the ratio of measured reflectance to the highest reflectance measured when the concentration of HWY336 was 1 mM.The results showed an increase amount of HWY336-MKK4 complex with time.In addition, the increase becomes greater as the concentration of HWY336 was increased.
The results in Figure 3 clearly confirmed the specific binding of HWY336 to MKK4, to produce an HWY336-MKK4 complex.The rate constant describing association of HWY336 and MKK4 was determined to be 66.31 M 21 s 21 .The dissociation constant (K d ) of ligand-protein binding was 3.2 mM for MKK4 and HWY336, which was comparable to those of MEK1 and its well-known selective inhibitors, U0126 (K d = 260.5 mM) and PD0325901 (K d = 561 mM) [15].

The activation loop of MKK4 and MKK7 is the HWY336 docking site
When we assessed whether HWY336 competed with a protein substrate or ATP for binding to MKK4 and MKK7, the inhibitory effect of HWY336 was unaffected by pre-incubation with ATP (Figure S2C and S3C in File S1), but was antagonized by MBP and JNK (Figure S2B and S3B in File S1).HWY336 no longer inhibited the kinase activity of MKK4 and MKK7 when MKK4 and MKK7 were pre-incubated with increasing concentrations of MBP and JNK (Figure S2B and S3B in File S1).These observations suggested that HWY336 either competes with a protein substrate for MKK4 and MKK7 binding, or at least blocks access of the substrate to its binding site.
To understand the basis of inhibitory selectivity of HWY336 to MKK4 and MKK7, we used molecular docking modeling of HWY336 and MKK4.As a first step in establishing a specific docking model for HWY336 binding to MKK4 and MKK7, the homology and phylogeny of these kinases were examined.In mammals, the MAPK cascades exist as two parallel pathways, MKK3/MKK6 and MKK4/MKK7 [16,17].Phylogenetic analysis based on sequence homology of p38, JNK3, ERK1, MEK1, MKK3/MKK6, and MKK4/MKK7 using ClustalW 2.0 (http:// www.ebi.ac.uk/Tools/clustalw2/index.html) [18], demonstrated that MKK3/MKK6 and MKK4/MKK7 are more closely related to each other than to the other kinases (Figure 4A).Multiple sequence alignments of MKK4/MKK7 and MKK3/MKK6 using ClustalW revealed a gatekeeper hinge region, and an Asp-Phe-Gly (DFG) motif.Moreover, we identified highly conserved Ser, Thr, and Tyr residues within the activation loops of MKK4/ MKK7 and MKK3/MKK6 (Figure 4B).
To further elucidate the inhibitory action of HWY336, we mapped its docking site by creating a three-dimensional model of MKK4 and MKK7.Since no crystal structure was currently available for MKK4, we used MKK7 (PDB code: 2DYL with resolution 2.45 A ˚) as a template for three-dimensional homology modeling for MKK4.MKK4 and MKK7 share 49% sequence identity.Therefore, the missing activation loop of MKK7 was first generated using the Modeller9v4 program [19].A homologybased structure for MKK4 was modeled using the three- dimensional coordinates from the modified MKK7 template and Modeller9v4.The three-dimensional alignment between the MKK7 template and MKK4 structure is shown in Figure S4A in File S1, demonstrating that the three-dimensional structures of MKK4 and MKK7 are similar.
Molecular docking generates and scores putative protein-ligand complexes.Given the crystal structure of a binding target, molecular docking automatically samples ligand conformation and protein-ligand interactions with a specified region of the protein surface.This method has been used successfully to identify active compounds by filtering out those that do not fit into the binding site.Because our data demonstrated that HWY336 treatment leads to complete inhibition of MKK4/MKK7 activity but not MKK3/MKK6 (Figure S1B in File S1), we examined the three-dimensional selective docking of HWY336 to MKK4/ MKK7 using the SYBYL 8.1 molecular modeling package installed on a Linux system.The structures of MKK4 and MKK7 were prepared using the biopolymer module of SYBYL 8.1.
The gatekeeper hinge region, DFG motif, and Ser/Thr and Tyr residues were highly conserved between MKK3/MKK6 and MKK4/MKK7 (Figure 4B).However, several sequence variations existed within the activation loop (Figure 4C).Therefore, we hypothesized that these varied residues may act as selectivity determinants among different MKKs.The active conformation in  a number of kinases involves phosphorylation of conserved residues, either Ser/Thr or Tyr.Thus, phosphorylation of these key residues may shift the balance between active and inactive states.In highly active kinases, DFG motifs adopt a highly conserved ''open conformation.'' We searched the possible ligand accessible cavities inside MKK4 as many as possible.Figure S5 in File S1 depicts the possible solvent pockets using SITE ID module of SYBYL 8.1.(FigureS5 in File S1).Site 1 is the ATP binding site and Site 2, the activation loop.Since HWY336 is a non-competitive inhibitor, ATP binding site was excluded.Among the remaining sites, only the site of activation loop has comparable size to HWY336.First, we docked ATP to the ATP-binding pocket and docked HWY336 near the activation loop.Based on the docking score, a docked pose was selected (Figure 4D).
HWY336 was positioned within the activation loop and near the phosphate binding region where the phosphate transformation process takes place.The docked pose for HWY336 in MKK4 showed hydrogen bonding with the conserved Thr 261 residue, which is important for the phosphorylation process (Figure 4C).A similar docked conformation was observed for MKK7 (Figure S4B in File S1).In our model, HWY336 showed a similar binding mode in the activation loop of MKK4/MKK7.HWY336 possibly formed a hydrogen bond with Arg 262 (Arg 292 of MKK7) within the activation loops of MKK4 and MKK7, suggesting an important role of this amino acid residue.Hydrophobic interactions between HWY336 and MKK4/MKK7 were also important for its MKK inhibition.Hydrophobic interactions between HWY336 and MKK4 (Figure 4D) demonstrated that a HWY336 side chain docked to the hydrophobic pockets of Pro 268 , Phe 305 , Pro 308 , and Val 313 .In the MKK7 structure (Figure S4B in File S1), a HWY336 side chain interacted with the hydrophobic Ala 297 , Ala 298 , Phe 336 , and Val 347 residues.The central 5-fused ring of HWY336 was positioned at the same location within MKK4 and MKK7, while groups attached to this ring had different orientations.
Three residues within the MKK4/MKK7 activation loop were important for selective inhibition by HWY336 Our structural model of MKK4 and MKK7 with HWY336 suggested that the activation loop was most likely a docking site for HWY336.Docking poses of HWY336 revealed similar binding between MKK4 and MKK7.The data also suggested that Gln 253 , Ile 258 , and Arg 262 within MKK4, and Arg 283 , Lys 288 , and Arg 292 within MKK7 differed from other highly conserved MKKs.Therefore, these activation loop residues were likely to be important for the selective docking of HWY336 (Figure 4C and Figure S4B in File S1).To confirm this hypothesis, we substituted Gln 253 , Ile 258 , and Arg 262 of MKK4 and Arg 283 , Lys 288 , and Arg 292 of MKK7 within the activation loop to Tyr, Val, and Met that correspond to residues in the activation loop of MKK3.MKK3, despite its homology to MKK4 and MKK7, was not susceptible to inhibition by HWY336.As expected, the mutants of activation loop, MKK4 mutant (Q253Y I258V R262M) and MKK7 mutant (R283Y K288V R292M), maintained similar kinase activity as the wild-type proteins because these residues were also present within the activation loop of MKK3 (Figure 5A  and B).
Because these changes enabled retention of kinase activity (Figure 5A and B), we hypothesized that the MKK4 mutant (Q253Y I258V R262M) and MKK7 mutant (R283Y K288V R292M) would display reduced sensitivity to HWY336 if these residues were important for HWY336 docking.Thus, we evaluated the effect of HWY336 on the phosphorylation activity of both the MKK4 and MKK7 mutants on JNK.Incubation with 10 mM HWY336 reduced the kinase activity of wild-type MKK4 and MKK7 but did not affect the kinase activity of MKK4 and MKK7 mutants (Figure 5C, D, E, and F).Even after treatment with 50 mM HWY336, the kinase activity of both the MKK4 and MKK7 mutants was unaffected, while no kinase activity was observed in the wild-type (Figure 5C, D, E, and F).These results demonstrated that the three amino acid residues within the activation loop of MKK4 and MKK7 were important for selective inhibition of the kinase activity of MKK4 and MKK7 by HWY336.These observations strongly supported the roles of Gln 253 , Ile 258 , and Arg 262 residues in MKK4, and Arg 283 , Lys 288 , and Arg 292 of MKK7 during HWY336 docking.

Discussion
In this study, we used a library of protoberberine derivatives to identify a selective inhibitor of the mammalian MAPK pathway.One of the compounds, HWY336, selectively inhibited MKK4 and MKK7 in vitro and in vivo by competing with protein substrates, suggesting that it associated with and/or modified the substrate binding site of MKK4 and MKK7.To understand the basis of selective inhibition of MKK4 and MKK7 by HWY336, we examined the direct binding of HWY336 with MKK4 by SPR and its docking with the predicted and established threedimensional structures of MKK4 and MKK7.The threedimensional modeling and docking studies suggested that HWY336 interacted with the activation loop of MKK4 and MKK7 adjacent to the substrate and ATP binding sites.Docking poses of HWY336 revealed similar binding between MKK4 and MKK7.Substituting the Gln 253 , Ile 258 , Arg 262 of MKK4 and Arg 283 , Lys 288 , Arg 292 of MKK7 within the activation loop to residues corresponding to those of MKK3 supported the possibility that the activation loop is a potential HWY336 docking site in MKK4 and MKK7.Moreover, we demonstrated that these conserved amino acids among MKK4 and MKK7 are important for selective inhibition of these kinases by HWY336.
Consistent with the inhibitory effect of HWY336 on mammalian MKK4 and MKK7, HWY336 also inhibited the stressactivated MAPKK Wis1 in Schizosaccharomyces pombe [20].Wis1 is a homologue of mammalian MKK4 and MKK7 in S. pombe, suggesting a similarity in the three-dimensional structures of these proteins, even though they only share 41% and 38% sequence homology, respectively.Interestingly, their sequence identities were much higher within the activation loop, and 16 out of 26 amino acids were identical (Figure S5C in File S1).Moreover, the three critical residues within the activation loop of MKK4 (Gln 253 , Ile 258 , and Arg 262 ) had corresponding counterparts in S. pombe Wis1 (Asn 465 , Ile 470 , and Asn 474 ; Figure S5C in File S1).Because Gln 253 and Asn 465 possessed an amide group as a side chain, other differences contributed by these residues most likely had a minimal effect on activation.The chemical property of Asn 474 in Wis1, which corresponded to Arg 262 in MKK4, contributed the greatest differences.While Asn is polar and uncharged, Arg is polar and basic.However, because these residues contain polar side chains, the change from Arg to Asn could be regarded as functionally conserved.The terminal guanidinium group of Arg 262 in MKK4 acts as a hydrogen bond donor in our structural modeling study.We would expect that the terminal amide group of the Asn 474 residue of Wis1 could also function as a hydrogen donor when it binds to HWY336.
The binding poses of ATP and HWY336 in our model indicated that HWY336 is a non-ATP competitive inhibitor of MKK4/MKK7.Our docking models strongly suggested that HWY336 inhibited MKK4/MKK7 phosphorylation within the activation loop or hindered substrate access to these protein kinases.Additionally, hydrogen bonding interactions between HWY336 and MKK4/MKK7 along with a washing experiment (Figure S2A and S3A in File S1) demonstrated that this compound bound reversibly and noncovalently.In general, phosphorylation at specific residues (Ser/Thr and Tyr) within the activation loop functions as a molecular switch for cellular communication.The phosphorylation process induces conformational changes such that the position of the highly conserved activation loop is altered to allow access to the substrate-binding domain.Thus, differences in the conformational state of the activation loop may play a major role in signaling specificity of each MAPKK in response to various stresses.
Our finding that HWY336 specifically inhibited the kinase activity of mammalian MKK4 and MKK7 in vitro and in vivo is significant, because no selective inhibitor has yet been reported or developed for these kinases.Although much effort has been devoted to developing selective inhibitors for each kinase in the mammalian MAPK pathway, specificity has been a major challenge.Some successful approaches targeted the ATP-binding site.For example, SB203580 and SP600125 blocked p38 and JNK kinase activity, respectively, by interacting with the ATP-binding domain [5][6][7].Alternatively, several non-ATP competitive inhibitors have also been developed, providing the opportunity to differentially target a specific component of the MAPK signal transduction cascade.PD98059 was the first specific MEK1/2 inhibitor that acted by binding to the inactive conformation of the enzyme to prevent its activation Raf [2,3].This inhibitor has also served as a standard pharmacologic tool for cellular studies of the ERK MAPK pathway.U0126, another MEK1/2 inhibitor, is also non-ATP competitive compound and is more potent than PD98059 [3].
In the present report, we have shown that HWY336 reversibly inhibited MMK4 and MKK7 by competing with protein substrates.HWY336 was of particular interest because its mechanism of inhibition is novel.Because kinases limit their substrates through specificity, and HWY336 competes with substrate(s) for binding, further study on the mechanism underlying the effects of HWY336 would provide important information that could be used to overcome the specificity barrier in the development of MAPK inhibitors.In addition, the high selectivity of HWY336 for MKK4 and MKK7 suggests that further modification of these compounds may lead to the development of selective inhibitors as therapeutics.

Figure 2 .Figure 3 .
Figure 2. HWY336 inhibits MKK4 and MKK7 selectively in a concentration-dependent manner both in vitro and in vivo.A, B) MKK4 and MKK7 were immunoprecipitated from HEK293T cells following MAPK pathway activation as described in EXPERIMENTAL PROCEDURES.The same quantity of kinase was used in each assay.The activity of MKK4 (A) and MKK7 (B) was assayed by measuring c-P 32 phosphorylation of a endogenous substrate, JNK, in the presence of increasing HWY336 concentration and quantified using ImageJ software to determine IC 50 .The average relative kinase activity compared to DMSO is plotted as a percentage.Error bars represent the standard deviations.C, D) HEK293T cells were treated with 600 mM D-sorbitol for 30 min before harvest.(C) HEK293T cells were treated with 12 mM of HWY336 for 0, 1, 2, 3, or 4 hrs, and their total lysates were analyzed by western blots.p-JNKs, p-p38, JNKs, and p38 were detected as described.(D) HEK293T cells were treated with various concentrations of HWY336 (3, 6, 9, 12 mM) for 3 hrs and each cell lysate was prepared.p-JNKs, p-p38, p-MKK4, JNKs, p38, and MKK4 were detected by western blot analysis of cell lysates.doi:10.1371/journal.pone.0091037.g002

Figure 4 .
Figure 4. MKK4 and MKK7 homology in mammalian MAPK pathways.A) Phylogenetic tree for human MKK4, MKK7, MKK3, MKK6, MEK1, p38, JNK, and ERK.B) Amino acid sequence alignment starting from the hinge region to the activation loop are shown for MKK3 (UniProtKB accession code: P46734), MKK6 (UniProtKB accession code: P52564), MKK4 (UniProtKB accession code: P45985), and MKK7 (UniProtKB accession code: O14733).Green denotes a highly conserved region among these MKKs.Residues highlighted in yellow designate sequence variations in the activation loop.C, D) Three-dimensional structure of MKK4 suggests that HWY336 interacts with the activation loop through hydrogen bonding.C) (top) Amino acid sequence variations within the activation loop of MKKs.(bottom) Proposed docked pose of ATP in MKK4.The arrow designates different amino acids that may determine MKK selectivity.The MKK4 structure is shown in the background with the activation loop (white) containing the varying amino acids (Arg 262 ) at the respective positions (generated with the Pymol program; www.pymol.org).D) Hydrophobic interactions between HWY336 and the MKK4 active site are shown.Hydrophobic residues within the active site are designated by the cap-stick model and HWY336 is displayed using transparent hydrophobic surfaces.The hydrophobicity index is displayed on the left, where brown and blue denote highly hydrophobic and hydrophilic areas, respectively.Pro 268 , Phe 305 , Pro 308 , and Val 313 interact with HWY336 side chains.HWY336 interacts with the activation loop of MKK4 through hydrogen bonding via the hydroxyl group of Thr 261 .doi:10.1371/journal.pone.0091037.g004

Figure 5 .
Figure 5. HWY336 does not inhibit the activity of MKK4 and MKK7 mutants of the proposed docking residues.Flag-tagged MKK4, MKK4-Q253Y I258V R262M, HA-tagged MKK7, and MKK7-R283Y K288V R292M were immunoprecipitated from activated HEK293 cells as described in Materials and Methods.Usage of the same amount of MKK4, MKK4 mutant, MKK7, and MKK7 mutant was confirmed by western blots with anti-flag antibody and anti-HA antibody, respectively.A, B) Kinase activity of immunoprecipitated (A) MKK4 and the MKK4 mutant, (B) MKK7 and the MKK7 mutant was assayed by measuring c-P 32 phosphorylation of JNK as a substrate.C-F) Effect of HWY336 on the kinase activity of (C, D) wild-type (top) and mutant (bottom) MKK4, (E, F) wild-type (top) and mutant (bottom) MKK7 was assayed after treatment with increasing concentrations of HWY336 (10, 30, 50 mM) or DMSO.D, F) Kinase activity of wild-type and mutant MKK4 and MKK7 with increasing concentrations of HWY336 shown in (C, E), was quantified by NIH ImageJ software.doi:10.1371/journal.pone.0091037.g005