A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean

Photoperiod and sucrose levels play a key role in the control of flowering. GmRAV reflected a diurnal rhythm with the highest expression at 4 h after the beginning of a dark period in soybean leaves, and was highly up-regulated under short-day (SD) conditions, despite of not following a diurnal pattern under long-day (LD) conditions. GmRAV-i (GmRAV-inhibition) transgenic soybean exhibited early flowering phenotype. Two of the FT Arabidopsis homologs, GmFT2a and GmFT5a, were highly expressed in the leaves of soybeans with inhibition (-i) of GmRAV under SD conditions. Moreover, the transcript levels of the two FT homologs in GmRAV-i soybeans were more sensitive to SD conditions than LD conditions compared to the WT plant. GmRAV-i soybeans and Arabidopsis rav mutants showed more sensitive hypocotyl elongation responses when compared with wild-type seedlings, and GmRAV-ox overevpressed in tobacco revealed no sensitive changes in hypocotyl length. These indicated that GmRAV was a novel negative regulator of SD-mediated flowering and hypocotyl elongation. Although sucrose has been suggested to promote flowering induction in many plant species, high concentration of sucrose (4% [w/v]) applied into media defer flowering time in Arabidopsis wild-type and rav mutant. This delayed flowering stage might be caused by reduction of LEAFY expression. Furthermore, Arabidopsis rav mutants and GmRAV-i soybean plants were less sensitive to sucrose by the inhibition assays of hypocotyls and roots growth. In contrast, transgenic GmRAV overexpressing (-ox) tobacco plants displayed more sensitivity to sucrose. In conclusion, GmRAV was inferred to have a fundamental function in photoperiod, darkness, and sucrose signaling responses to regulate plant development and flowering induction.


Introduction
In many plant species, flowering time is strongly influenced by environmental factors where photoperiod plays a prominent role [1]. Plants perceive light through its phytochromes and cryptochromes, which transfer the signal to the plant internal circadian clock system. There is an increasing evidence for conservation of flowering pathways between many plant species. Flowering time is regulated by multiple and to some extent redundant pathways that can promote or delay flowering [2]. Arabidopsis thaliana, a model organism, is a long-day plant, and there are many pathways, such us photoperiod, vernalization, gibberellic acid and autonomous reactions, involved in control of its floral transition [3][4][5][6]. In contrast, soybean (Glycine max) is a short-day plant, and photoperiod controls its duration in both pre-and post-flowering phases [7]. Therefore, photoperiod is an important environmental cue that determines flowering time in soybean [8]. The term 'critical photoperiod' is described as the duration of daylight period under which the plant is induced to flower, and determines plant transition from vegetative to reproductive stage. Sensitivity to photoperiod limits the adaptation of soybean to a wider range of latitude [9]. The identification of the genetic components contributing to the photoperiodic control of flowering time in soybean was recently limited.
Florigen (FT) is a hypothetical leaf-produced signal that moves from phloem to induce flowering at shoot apex. The expression of FT gene (the flowering integrator genes, FLOWERING LOCUS T [10], and its orthologs are critical for flowering in plants [11,12]. Two soybean FT homologs (GmFT2a and GmFT5a) have florigenlike functions and their transcript levels are upregulated under SD conditions (SDs) [13].
Not only carbohydrates provide energy and carbon sources for plants, but also act as essential regulators during their growth and development [14,15], as evidenced by the variety of sugar sensing and signaling mechanisms that have been uncovered [16,17]. Carbohydrates seem to regulate many essential processes, including photosynthesis, sucrose synthesis and degradation, flowering, and senescence [18,19].
There has been a certain amount of evidence suggesting that sucrose promotes flowering in most species [20]. In Arabidopsis, the induction of flowering in wild-type plants by LDs causes an early and transient increase in sucrose export from leaves. The efficiency of floral induction by a single LD is reflected by the amplitude of an increase in exported sucrose [21]. Rolda'n et al. reported that in vitro culture of plants on medium containing 1% (w/v) sucrose, partially rescued the phenotypes of late-flowering mutants [22]. In contrast, Zhou et al. reported that high levels of glucose in the medium delayed flowering in Arabidopsis [23]. Masa-aki et al. also analyzed the effects of sugar on development and floral transition [24]. In an early flowering mutant tfl1, 5% (w/v) sucrose in the medium delayed floral transition. It was concluded that the inhibition was caused by metabolic rather than its osmotic effects. Recently, King et al. reported that FT and sucrose may regulate flowering as 'a florigen' in plants [25].
Despite their metabolic role, glucose and fructose play additional signaling functions in plant cells [26]. Oligosaccharides derived from the cell wall also function as signals in the processes of regulation of hypocotyl elongation [27], fruit ripening [28] and defense mechanisms to pathogens [29]. Also, root growth was considerably more sensitive to carbon source than hypocotyl elongation [30]. In this study, the effects of sugars on a range of growth and developmental parameters in Arabidopsis thaliana, tobacco and soybean were measured. The effects of sugars on growth and developmental processes in plants at earlier stages of vegetative development and the flowering timing were also investigated.
In Arabidopsis, the RAV subfamily belongs to one of the largest and most diverse family of transcription factors AP2/ERBP. RAV proteins function in the involvement in cold tolerance, dehydration, and circadian rhythm clock. GmRAV (DQ147914) [31,32] was one of four RAV2-like paralogues in the soybean genome. GmRAV may be a complete functional orthologue of any AtRAV2 family member [33].
In this study, GmRAV was overexpressed in transgenic tobacco and inhibited in transgenic soybean, which showed that GmRAV was a responder in the photoperiodic control of flowering time and sugar signaling. We found that GmRAV transcript exhibited a circadian rhythm under SDs and decreased significantly in leaves by exogenous sucrose application. A detailed phenotypic characterization, along with genetic and physiological analysis, indicated that GmRAV was inferred to be a signaling component involved in regulation of plant development and flowering time.

Plant Materials and Growth Conditions
Arabidopsis thaliana Columbia (Col-0) ecotype was used in this study as wild-type plant (Lehle Seeds, Round Rock, TX). The Salk T-DNA knockout mutant line of AtRAV (At1g25560; SALK_029626c) was obtained from the Arabidopsis Biological Resource Center (ABRC). GmRAV-i soybean, GmRAV-ox tobacco and GmRAV promoter::GUS transgenic Arabidopsis seeds were provided by our lab (Northeast Agricultural University, Harbin, China) [33]. The primer pairs for transgenic plants detection were listed in Table 1. Arabidopsis seeds were surface sterilized, placed in Petri dishes containing solid Murashige and Skoog (MS) medium, and stratified for 3 days at 4uC. Subsequently, the seedlings were placed in a vertical orientation in the growth chamber at 22uC under LDs (16 h/8 h light/dark). T4 generation GmRAV-ox tobacco and T6 generation transgenic GmRAV-i soybean [33] were grown in a growth chamber at 25uC, and illuminated with 200 mmol?m 22

RNA Isolation and Quantitative Real-time RT-PCR (qRT-PCR) Analysis
Total RNA was extracted from soybean and Arabidopsis seedlings with RNAiso Plus Kit (TaKaRa, Japan). The total RNA was reverse-transcribed into first-strand cDNA in a 20 mL volume with PrimeScript RT reagent Kit (TaKaRa, Japan). qRT-PCR analysis was carried out using SYBR Premix Ex Taq II   were included as inner references for soybean and Arabidopsis genes. The total RNA was used as templates in qRT-PCR reactions with the primers of GmRAV, GmFT2a, GmFT5a and AtLEAFY genes. The primer pairs were listed in Table 2. PCR reactions were performed according to the manufacturer's instructions on the Chromo 4 real time DNA amplification system (BioRad, USA). Data were analyzed using the comparative Ct method. Further qRT-PCR analysis were performed as described above. The analysis were done using the DNA Engine Opticon 2 System (MJ Research, USA). The sequences reported in this paper have been deposited in the GenBank/EMBL/DDBJ database with accession numbers AB550122 (GmFT2a), AB550126 (GmFT5a) for cDNA sequences of soybean cultivar 'Dong Nong 50' and Genbank accession number AF010190.2 (AtLEAFY) for cDNA sequences of Arabidopsis.

GUS Assays
GUS activity was assayed in T3 transgenic Arabidopsis plants. GUS histochemical staining and GUS activity measurements (using about 40-50 seedlings in each sample) were carried out following the procedures described by Jefferson et al. [34].

Sucrose Stress Assay
The sucrose sensitivity assay for hypocotyl elongation was carried out by germinating the wild-type and rav transgenic seeds. The change in root and hypocotyl length was used as a measure to check the sensitivity of the plants using the Image-J program (http://rsb.info.nih.gov/ij/docs/menus/file.html) after 15 days.
For flowering assay, the wild-type and transgenic seeds were grown on MS medium supplemented with different concentrations of sucrose during their lifetime. Plants were growing on MS with 2% and 4% (w/v) sucrose, and after 15 days were transferred into soil till flowering. Flowering time was measured by scoring the time from sowing to first flower.

Statistical Analysis
Data was presented as means 6 standard error of means. The statistical comparisons were made using Student's t test at p,0.01 or p,0.05.

The Accumulation of GmRAV Transcript is Regulated by Photoperiod and Darkness
Transcription profiles of GmRAV were analyzed in various tissues of 'Dong Nong 42' soybean grown under inductive SDs at 12 h after dawn by quantitative real-time RT-PCR. GmRAV mRNA was present in all organs examined, including leaf (Tl), trifoliate leaf (TL), stem (St), root (RO), pod (PO), flower bud (FB), and immature seed (IS). In SDs, the mRNA abundance of GmRAV was the highest in trifoliate leaves and the lowest in pods (Fig. 1A).
The diurnal circadian rhythm of GmRAV expression was examined by quantitative real-time RT-PCR in trifoliate leaves sampled at 25 d (transferred at 10 d). GmRAV transcript exhibited a diurnal circadian rhythm under SDs, suggesting that their expression was partly regulated by circadian clock genes. The expression level of GmRAV increased slightly 4 h after dawn, reaching a peak 4 h after the beginning of the dark period and decreased toward dawn, reaching the lowest 4 h before dawn under SDs (Fig. 1B). The amplitude of GmRAV mRNA increased significantly under SDs when compared with LDs.
The abundance of the GmRAV mRNA in leaves during the shift from SDs to LDs was investigated (Fig. 1B). The time coursedependent expression patterns of GmRAV were also analyzed in 'Dong Nong 47' plants grown under SDs and LDs using RNAs isolated from trifoliate leaves that were sampled at 4 h after dusk. The levels of GmRAV transcripts under SDs were relatively low at 20 DAE but increased sharply to their maximum levels at 23 DAE and thereafter decreased until 45 DAE (the time of flower bud formation) (Fig. 1C). In contrast, under LDs, the transcript levels of GmRAV increased slightly at 23 DAE, and thereafter decreased showing lower levels than that under SDs at all the times (Fig. 1C). Overall, the gene expression studies indicated that GmRAV was SD-inducible gene in soybean leaves.
In addition, promoter activity of GmRAV was measured to determine whether continuous darkness could increase GmRAV expression like in SDs (Fig. 1B). In 7-day-old seedlings, GUS expression of GmRAV promoter via histochemical GUS assay was more predominantly detected under continuous darkness than in continuous light conditions in both cotyledons and hypocotyls (Fig. 1D). These results suggested that GmRAV was SD and darkness-inducible gene.

Effects of GmRAV on Photoperiod Controlling of Flowering Time FT Homologs in GmRAV-i Transgenic Soybean
The transcript abundance of GmRAV was affected by day length in soybean leaves, the diurnal phase of GmRAV mRNA expression was regulated by the circadian clock, and higher levels of GmRAV mRNA were accumulated under SDs than under LDs. To examine whether GmRAV acted in a photoperiod functional context, the flowering time in WT and GmRAV-i soybeans under SDs and LDs were analyzed. The early-flowering phenotype soybean mutant GmRAV-i was observed despite of the day length in both LDs and SDs compared to the WT ( Fig. 2A). Apart from their flowering-time phenotype, GmRAV-i soybeans also displayed complex pleiotropic alterations of vegetative development. Earlier emergence, reduced numbers of branches and leaves, longer petioles, larger leaves, increased apical dominance, and earlier flowering and maturity were the major phenotypic effects of GmRAV-i in all T6 generation plants in both SDs and LDs ( Fig. 2A, Table 3).
In  (Fig. 2C,  D). Moreover, the transcript abundance of GmFT2a and GmFT5a was highly affected in GmRAV-i transgenic soybean leaves compared to the wild-type seedlings under SDs than under LDs (Fig. 2E, F). The results indicated that GmRAV was a SD-inducible flowering repressor in the flowering response of SD-induced soybeans by repressing positive regulator GmFT2a and GmFT5a gene expression. This work therefore described the conservation of components and sequence order of a pathway controlling flowering in response to day length. It revealed that the promotion of flowering in short days in GmRAV-i soybean resulted from the repression of GmFT2a and GmFT5a by GmRAV (Fig. 2G).

Effects of GmRAV on the Photoperiod Controlling by Hypocotyl Elongation
To further investigate whether GmRAV was SD-inducible, the test of hypocotyl elongation was conducted in LD and SD-grown knock-out soybean mutant GmRAV-i, and Arabidopsis rav mutants and wild-type plants. GmRAV-i soybeans showed SD -mediated hypocotyl elongation responses compared to the wild-type seedlings (Fig. 3A, B). In GmRAV-i soybeans, GmRAV enhanced SD-mediated hypocotyl elongation response. In comparison, Arabidopsis rav mutant displayed the same hypocotyl elongation response to SDs (Fig. 4A). This was evident when grown in LDs or SDs (Fig. 4B, C). These results indicated that GmRAV also played a negative role in SD-mediated regulation of hypocotyl elongation.

Effects of Sucrose on the Flowering of Arabidopsis Rav Mutant
Masa-aki et al. reported that 2 weeks culture was enough to observe the negative effects of high levels of sucrose on floral transition [24]. Therefore, wild-type plants and Arabidopsis rav mutant seedlings were grown in culture medium containing 2% (w/v) and 4% (w/v) sucrose for 2 weeks, respectively and then were transferred to soil. Flowering time of Arabidopsis WT and rav mutants were examined on MS medium containing 4% sucrose in LDs. In comparison to the plants that were grown on 2% sucrose plates, rav mutants showed a 2-day delay in flowering time, whereas WT plants showed 4-day delay (Fig. 5A). These results supported our hypothesis that Arabidopsis rav mutants responded to sucrose signaling in plant growth and development. Sucrose affecting flowering time was also observed in the developmental phenotypes (Fig. 5B). Overall, these results indicated that AtRAV participated in the regulation of high levels of sucrose-dependent flowering response.
To investigate the reason why high concentration sucrose could delay flowering, different levels of FT, SOC1/AGL20 and LEAFY (LFY) expressions were analyzed by reverse transcriptase (RT)-PCR in Arabidopsis WT plants and rav mutants grown respectively on media with 2% and 4% sucrose for 15 d. Both expression levels of FT and SOC1/AGL20 in Arabidopsis rav mutants as well as WT plants on media with 2% (w/v) were identical with on media with 4% (w/v) sucrose under LD conditions (data not shown). LFY was expressed in the leaf primordium before the transition to flowering. In this study, LFY expression levels were reduced under the supplementary of 4% (w/v) sucrose compared to 2% (w/v) sucrose (Fig. 5C). The results suggested that increased concentration of sucrose could lead to decrease the expression of LFY gene. It also showed that high concentration of sucrose in growth media delayed the flowering time in Arabidopsis. Moreover, transcript level of LFY was greatly reduced in rav mutants than in WT plants on media containing high level of sucrose under LDs. The results displayed that GmRAV delayed flowering in high level of sucrose by regulating the expression of LFY.

The Responses of Arabidopsis Rav Mutant, GmRAV-ox Tobacco and GmRAV-i Soybean Seedlings to Exogenous Sucrose
To analyze the possible function of GmRAV in response to sucrose stress, we studied the responses to exogenous sucrose application using Arabidopsis rav, GmRAV-ox tobacco, and GmRAV-i soybean plants. Sensitivity of Arabidopsis rav mutants and GmRAV-ox tobacco seedlings in response to sucrose was tested in root and hypocotyl by growth inhibition assays. The hypocotyl and root lengths of GmRAV-ox tobacco and WT plants were inhibited by both concentrations of sucrose (2% and 4%) (Fig. 6A), but GmRAV-ox tobacco were more remarkably reduced compared to WT plants (Fig. 6B, C). These results suggested that GmRAV-ox tobacco were more sensitive to sucrose than WT plants in the assays of hypocotyls and roots growth inhibition. Fig. 7A showed that the hypocotyl and root lengths of Arabidopsis rav mutants and WT plants were inhibited by both concentrations of sucrose. However, inhibited extent of rav mutants and WT plants by sucrose in hypocotyl and root inhibition assays was identical (Fig. 7B, C). Likewise, the growth in soybean GmRAV-i and WT plants in terms of hypocotyl lengths, main root length and the number of lateral roots was all inhibited by both concentrations of sucrose (Fig. 8A). Hypocotyl, root length, and the number of lateral roots of GmRAV-i soybeans were less inhibited by sucrose than in WT plants (Fig. 8 B-D). Therefore, GmRAV-i seedlings exhibited insensitive phenotypes, as compared with the WT plant, during sucrose-mediated root and hypocotyl growth inhibition. Overall, in agreement with the GmRAV-ox tobacco results, the Arabidopsis rav mutants and the GmRAV-i soybean seedlings showed a significantly decreased sensitivity to sucrose by root and hypocotyl growth inhibition assays.
The addition of chemicals to a medium changes both the chemical composition and the osmotic potential of medium. To identify whether the negative effects of sucrose on development and flowering were due to metabolic or osmotic factors, we examined the effects of both mannitol and sorbitol. These two sugar alcohols were widely used as the osmotic controls in plant. Therefore, a combined osmotic control value was calculated from both mannitol-and sorbitol-grown plants. Sugar effects were compared to the combined osmotic control value. Plants grown in the absence of any supplemental carbon (sugar alcohol or sucrose) were also measured for each parameter in order to observe the overall effect of increasing osmolarity. Increasing osmolarity of the growth media by sugar alcohols inhibited the plant growth when compared to untreated media. The behavior of Arabidopsis rav mutant, GmRAV-ox tobacco and GmRAV-i soybean seedlings with 200 mM sugar alcohols on MS media showed no difference from that of the WT seedlings in hypocotyl, root and the number of lateral roots growth inhibition assay (Fig. 9 A-E). However, rav mutants and soybean GmRAV-i in terms of hypocotyl lengths, main root length and the number of lateral roots of development that exhibited insensitive phenotypes were less inhibited by 200 mM sugars (6.8%) (Fig. 9A, C-E), which indicated RAV gene played a negative role in sucrose-mediated regulation of hypocotyl and root elongation due to metabolic effects rather than osmotic effects.

GmRAV is a Novel Negative Regulator of SD-mediated Flowering and Hypocotyl Elongation
The time of flowering induction determines to a large extent the reproductive success of plants. Plants integrate diverse environmental and endogenous signals to ensure the timely transition from vegetative to flowering period. In many plant species, floral transition is strongly controlled by the circadian clock. The clock with a period close to 24 h serves to coordinate diurnal rhythms with physiology and behavior. GmRAV belongs to the RAV protein family containing two domains: the AP2 and the B3 DNAbinding domain. Given that long-day plant Arabidopsis TEM1, TEM2 and chestnut CsRAV1 genes were circadian regulated [35,36], we examined the possibility that soybean gene was rhythmically expressed in short-day plant soybean leaves. Higher level of GmRAV transcripts was accumulated in soybean leaves in SDs than in LDs which was almost suppressed, and it was also regulated by the circadian clock. The GmRAV mRNA reached a peak 4 h after the beginning of the dark period in SDs, whereas CsRAV1 mRNA peaked at noon, TEM1 and TEM2 peaked at dusk in LDs. This different time of expression in day suggests that although RAV gene shows high homology among different daylength plants, it may play different roles in different day lengths. Furthermore, the time course-dependent expression pattern of GmRAV was analyzed in 'Dong Nong 47' plants grown under SDs and LDs, and showed that the level of GmRAV transcript in SDs was higher than in LDs till flowering, confirming that GmRAV was SD-inducible gene.
We further analyzed the function of repressing flowering of GmRAV gene based on the earlier flowering phenotypes of GmRAVi soybeans than WT under both SDs and LDs. In Arabidopsis, TEM1 and TEM2 also acted as direct FT repressors and repressed flowering under LD and SD conditions [35,37]. Likewisely, the mRNA levels of GmFT2a and GmFT5a were also examined in WT and GmRAV-i soybeans under SDs, and they were evidently enhanced in earlier flowering GmRAV-i soybeans, which indicated that GmRAV played a negative role by repressing FT genes in the SD-mediated photoperiod control of flowering in soybean, whereas in Arabidopsis, TEM1 repressed flowering by repressing FT genes in LDs [35]. We speculated that a genetic pathway Furthermore, in photoperiod control of hypocotyl elongation assays, both GmRAV-i soybeans and Arabidopsis rav mutants were hypersensitive in SD-mediated promotion of hypocotyl elongation. Therefore, we concluded that the RAV role was also conserved in the photoperiod control of hypocotyl elongation in soybean and Arabidopsis.

GmRAV Affected Development and Flowering Time in the Presence of Exogenous Sucrose
Carbohydrates are thought to play a crucial role in the regulation of flowering. The relation between sugar metabolism/ signaling and floral transition received extensive attention lately [25]. Sugar signaling was of great importance in flowering time control, which directly affected yield [38,39]. The work of Heyer et al. already provided clear evidence that flowering time control is strongly influenced by modifying sugar balances in the apex [40]. Several sucrose signaling insensitive Arabidopsis mutants have been identified based on the effect of high levels of external sugars on seedling growth and development such as cai (carbohydrate insensitive) [41], isi (impaired sugar induction) [42], lba (low levels of b-amylase) [43], rsr (reduced sugar response) [44], sis (sugar insensitive) [45], sun (sucrose uncoupled) mutants [46]. For example, sig (sucrose insensitive growth) mutant was selected on media containing 350 mM sucrose [47]. Similarly, Arabidopsis rav mutants and soybean GmRAV-i in terms of hypocotyl lengths, main root length and the number of lateral roots of development which exhibited insensitive phenotypes were less inhibited by 200 mM sugars.
To ensure uniform sugar responses, the initial mutant isolation screens were mostly performed on media containing high sucrose concentrations, raising concerns about the physiological relevance of sugar regulation. Moreover, the phenotypes could also be influenced by osmotic stress in the media. In this study, we treated Arabidopsis rav mutants, GmRAV-ox tobaccos and GmRAV-i soybeans with 200 mM supplemental carbon sources, and observed that transgenic plants and WT were almost uniformly inhibited by only supplemental sugar alcohols. A high concentration of sucrose added into basal medium also inhibited development both WT and transgenic plants. But we found the behavior of Arabidopsis rav mutant, GmRAV-ox tobacco and GmRAV-i soybean seedlings with 200 mM sugar alcohols on MS media showed no difference from that of the WT seedlings in hypocotyl, root and the number of lateral roots growth. Thus, the specific effects of sucrose treatment could be attributed to the chemistry of the sucrose itself rather than to osmotic effects of the sucrose, which indicated RAV gene played a negative role in sucrose signaling due to metabolic effects rather than osmotic effects.
In Arabidopsis, the delay in flowering time caused by high concentrations of glucose in media was previously reported [23]. Sugar seemed to affect a specific part of the vegetative phase, rather than all phases [24]. Flowering time of Arabidopsis rav mutants were less reduced than WT under high concentration of 4% sucrose condition, which showed Arabidopsis rav mutants were also insensitive to sucrose in flowering time. Moreover, the expression of LFY was much more down-regulated in Arabidopsis WT plants compared to rav cultivated in high concentrations of sucrose, indicating that RAV might be also a positive regulated factor in flowering time inhibition assays.
In summary, as an important process in plant reproduction, flowering time is finely controlled by complex network. GmRAV was both involved in negative regulation of the photoperiodic control of flowering time responses and positive regulation of sucrose control of flowering time. Figure 8. Response of WT and GmRAV-i soybean seedlings to sucrose. A and B, Relative hypocotyl and root growth in response to various concentrations of sucrose. Hypocotyl length, as a percentage of the untreated control, of seedlings grown on sucrose. The length of hypocotyl of soybean seedlings grown without sucrose or glucose was 57.1761.30 mm for the WT and 61.1761.97 mm for GmRAV-i soybean. Root length, as a percentage of the untreated control, of seedlings grown on sucrose. Root length, of seedlings grown without sucrose or glucose was 60.3361.30 mm in the WT and 65.6761.97 mm in GmRAV-i. Error bars represent the SE. C, Relative number of lateral roots in response to various concentrations of sucrose. The number of lateral roots, as a percentage of the untreated control, of seedlings grown on sucrose. Error bars represent the SE. The number of lateral roots of seedlings grown without sucrose and glucose was 17.8360.75 for the WT and 21.1760.98 for GmRAV-i soybean. The number of lateral roots of 20-30 seedlings was scored for each treatment. *Differences in comparison to the non-transgenic lines at 0.01,P,0.05. D, Phenotypes of 7-day-old WT and GmRAV-i soybean seedlings on MS medium containing 2% and 4% sucrose. *Differences in comparison to the nontransgenic lines at 0.01,P,0.05 (Student's t test). doi:10.1371/journal.pone.0089145.g008