Comparative Analysis Reveals Distinct and Overlapping Functions of Mef2c and Mef2d during Cardiogenesis in Xenopus laevis

The family of vertebrate Mef2 transcription factors is comprised of four members named Mef2a, Mef2b, Mef2c, and Mef2d. These transcription factors are regulators of the myogenic programs with crucial roles in development of skeletal, cardiac and smooth muscle cells. Mef2a and Mef2c are essential for cardiac development in mice. In Xenopus, mef2c and mef2d but not mef2a were recently shown to be expressed during cardiogenesis. We here investigated the function of Mef2c and Mef2d during Xenopus laevis cardiogenesis. Knocking down either gene by corresponding antisense morpholino oligonucleotides led to profound heart defects including morphological abnormalities, pericardial edema, and brachycardia. Marker gene expression analyses and rescue experiments revealed that (i) both genes are required for proper cardiac gene expression, (ii) Mef2d can compensate for the loss of Mef2c but not vice versa, and (iii) the γ domain of Mef2c is required for early cardiac development. Taken together, our data provide novel insights into the function of Mef2 during cardiogenesis, highlight evolutionary differences between species and might have an impact on attempts of direct reprogramming.


Introduction
In the mouse, the heart arises from two populations of cells referred to as the first and second heart field, respectively [1][2][3].Cells of the first heart field (FHF) localize in the cardiac crescent and later form the linear heart tube that grows by continuous addition of cells from the venous as well as the arterial pole.These newly added cardiac cells originate from the so-called second heart field (SHF) that is characterized by the expression of transcription factors Islet-1 (Isl1) and T-box factor 1 (Tbx1) [4].From lineage labeling studies, it is known that cells of the SHF contribute to most of the right ventricle and the outflow tract and contribute to the aortic sac, the endocardium, the ventricular and atrial septum as well as to the atrial myocardium whereas the other parts of the heart are mainly derived from cells of the FHF [5].Data gained from work in mouse ES cells suggest that the FHF and the SHF are established from a common cardiac progenitor cell (CPC) population.
Cardiac development in Xenopus starts at the onset of gastrulation with the induction of two bilaterally located cardiac progenitor cell populations on either side of the Spemann Organizer [6,7].During gastrulation, these cells migrate towards the anterior-ventral side of the embryo and fuse to a single common CPC population located adjacent and posterior to the cement gland anlage [8].During further development, this CPC population becomes more and more heterogenic, so that at stage 24, two populations of cells can be distinguished by differential gene expression.Lineage labeling experiments have revealed that these two separate cell populations correlate to the first and the second heart field in mice, respectively.At this stage, cells of the Xenopus FHF already express genes indicating cardiac differentiation such as cardiac troponin (tnni3) and myosin heavy chain (myh6).Upon further development, these cells will later contribute to forming the single ventricle, the two atria and the inflow tract [8].Cells of the SHF are localized anterior to the first heart field adjacent to the cement gland and express isl1 and tbx1 as well as bone morphogenetic protein 4 (bmp4).These cells have been shown to develop into the outflow tract [8].
Mef2 (myocyte enhancer factor 2) proteins belong to the MADS (MCM1, agamous, deficient, serum response factor) family of transcription factors.Members of the Mef2 family contain a common MADS-box and a Mef2-type domain at their Nterminus.The MADS-box serves as a minimal DNA-binding domain that requires the adjacent Mef2-type domain for dimerization and high-affinity DNA binding [9][10][11].These proteins also contain a transactivation domain at the C-terminus which is involved in the regulation of transcriptional activity.The four vertebrate Mef2 genes -referred to as Mef2a, b, c and dare expressed in precursors of the three muscle lineages as well as in neurons [10].The vertebrate Mef2 gene products share about 50% overall amino acid identity and are about 95% similar throughout the highly conserved MADS-box and Mef2-type domain.In contrast, they are more divergent in their C-terminal regions [10,11].Additionally, each Mef2 gene gives rise to multiple isoforms through alternative splicing that are expressed in different embryonic and adult tissues [12].In mammals, three major exons are alternatively spliced in Mef2 genes: the two mutually exclusive exons a1 and a2, and the short exons b and c.The function of these isoforms depends on the exon composition after splicing.In particular the amino acids coded by the c exon have been shown to function in gene repression [12].The predominantly expressed Mef2c isoform in the adult murine heart as well as during differentiation of mouse C2C12 into myocytes is lacking this c domain [12].For Xenopus mef2c, three exons are alternatively spliced: two exons corresponding to the mammalian b and c exons, as well as the so-called d exon.No mef2d splice variants were detected in Xenopus [13].
Mef2 transcription factors regulate a wide array of genes including numerous muscle-specific enzymes, structural proteins and other transcription factors, e.g.MyoD or MHCa.Loss of function mutations in the single mef2 gene in the Drosophila embryo result in an abnormal development of all muscle cell types including cardiomyocytes [14].In mouse, Mef2a, c and d are detected in the cardiac mesoderm [15].Mef2d knock out mice do not exhibit any obvious embryonic phenotypes and a Mef2b knock out mouse has not yet been generated.In contrast, Mef2a or Mef2c null mice show defects in cardiac development [16,17].In particular, Mef2a knock out mice die perinatally from an array of heart defects including severe cardiac cytoarchitectural defects and right ventricular chamber dilation [18].Mef2c null mice die at embryonic day 10.5 due to failure in cardiac development, e.g.cardiac edema, defects in cardiac looping, brachycardia and deficits in the development of the right ventricle.The animals also exhibit vascular defects [17,[19][20][21].Meanwhile, Mef2c has been integrated into a complex gene regulatory network acting through early cardiogenesis [22].The important role of Mef2c during cardiac development was also recently demonstrated by showing that Mef2c together with other transcription factors can cooperatively reprogram adult mouse tail-tip and cardiac fibroblasts into beating cardiomyocyte-like cells in vitro and in vivo [23][24][25].In adult mice, Mef2a and Mef2d represent the major isoforms expressed [26].Gain and loss of function studies in genetically modified mice indicated an important function of Mef2d for stress-dependent cardiac growth.Overexpression of Mef2d also resulted in reactivation of fetal cardiac gene expression in the adult heart [26], whereas loss of Mef2a in contrast is lethal [18].Understanding the molecular function of these transcription factors in more detail is thus crucial for gaining deeper insights into cardiac development as well as cell reprogramming.
mef2c and mef2d expression was recently shown in cardiac tissue of Xenopus laevis embryos whereas mef2a is not expressed in the heart [8,13], suggesting a role of Mef2c and Mef2d during Xenopus heart development.This is in contrast to the situation in mouse where, as discussed above, Mef2a and Mef2c but not Mef2d knock out mice display an embryonic cardiac phenotype.By loss of function studies, we show here for the first time that both Mef2c and Mef2d are required for cardiogenesis in Xenopus.In addition, knocking down Mef2c or Mef2d leads to a down-regulation of characteristic genes in both, the first and second heart field.Furthermore, we observed that depletion of Mef2c can be rescued by Mef2c and MEF2D, while Mef2c cannot compensate for the loss of Mef2d in Xenopus embryos.Equally important, we also show for the first time that the c domain of Mef2c is essential for correct cardiac development.

mef2c and mef2d are expressed in the developing heart in Xenopus laevis
To initiate this study we first analyzed available sequence information on mef2 genes in Xenopus laevis and Xenopus tropicalis.Comparing the synteny of the Mef2a, Mef2c, and Mef2d genes in the human and the mouse genomes with the corresponding homologs in Xenopus confirmed that the available genomic sequences and EST clones code for the corresponding homologs in Xenopus (Fig. 1 A, C and D, Table S1) and that the current annotations of these mef2 family members in Xenopus are correct.For mef2b, no EST sequences were reported so far and no homolog has been identified in the Xenopus tropicalis genome.Therefore, we analyzed the genomic region of the Xenopus tropicalis genome where mef2b should be localized in more detail.In humans, Mef2b is localized on chromosome 19 and the neighboring genes are well known.This synteny is well conserved between the human and the mouse genomes (Fig. 1 B, Table S1).Using this information, we were able to identify one scaffold that should contain the genomic information for Xenopus mef2b.Whereas the orthologs of the mef2b flanking genes could be identified on this scaffold, no sequences coding for mef2b were found.Interestingly, a region spanning about 277 kb was inverted in comparison to the human and mouse genomes.One DNA break point required for this inversion covers the region where mef2b should be located.This observation and the lack of any mef2b EST suggest that the gene coding for mef2b was lost in Xenopus during evolution.
RT-PCR experiments revealed that Xenopus mef2c and mef2d transcripts are maternally supplied and expressed during the entire embryogenesis (Figure S1 A, B).We next confirmed the spatiotemporal expression pattern of mef2c and mef2d during Xenopus laevis cardiac development as previously published [8,13].Xenopus mef2c transcripts were faintly detected in the common cardiac progenitor cell (CPC) population at stage 20 whereas mef2d transcripts were strongly detected in CPCs (Figure S1 C, L).During further development, mef2c and mef2d are continuously expressed in cardiac tissue (Figure S1 D-M, K-T).At stages 28 and later, both genes are expressed in the first heart field (FHF) region (Figure S1 F-K, O-T), whereas only mef2c is expressed in the more lateral region of the second heart field (SHF) (Figure S1 G, red arrowhead).Transverse sections revealed that mef2c is expressed in the myocardium (arrowhead) as well as in the endocardium (arrow) during tailbud stages (Figure S1 h), while stronger mef2d expression is restricted to the myocardium at this time point (Figure S1 q, arrow).At stage 36, expression of both, mef2c and mef2d, was observed in the myocardium (Figure S1 j, s, arrows).

Loss of either Mef2c or Mef2d results in cardiac malformations
For loss of function studies we relied on morpholino oligonucleotide (MO) based antisense strategies.To test the binding efficiency and functionality of the MOs used, the MO binding sites of Xenopus mef2c (here denoted as xmef2c), and Xenopus mef2d (xmef2d) were cloned into the pCS2+ expression vector in front of and in frame with the GFP gene.The RNAs of these constructs were bilaterally co-injected with either Control MO or Mef2c MO or Mef2d MO into two-cell stage embryos.GFP expression was monitored at stage 20 (Figure S2).Co-injection of the xmef2c-GFP fusion construct together with Mef2c MO blocked the translation of GFP, whereas co-injection of a Control MO had no effect on the expression.Similar, co-injection of the xmef2d-GFP fusion construct together with Mef2d MO also blocked the translation of GFP.In order to rescue the observed phenotypes seen after knocking down either Mef2c or Mef2d, we relied on RNA coding for murine Mef2c or human MEF2D that were not targeted by either MO due to sequence differences in the 59UTR (mMef2c) or due to introduced silent point mutations in the coding sequence (hMEF2D).Again, the efficiency was tested as described above.The expression of mMef2c-GFP or hMEF2D-GFP was not blocked by Mef2c MO.The Mef2d MO did not have an inhibitory effect on hMEF2D-GFP or mMef2c-GFP (Figure S2).Taken together, these results support the idea that (I) both MOs specifically block the translation of the corresponding Xenopus mef2 gene, (II) Mef2c MO neither inhibits mMef2c nor hMEF2D translation and (III) the Mef2d MO does not interfere with mMef2c or hMEF2D expression.In conclusion, RNA coding for murine Mef2c and human MEF2D can be used as gene specific rescue constructs.
To interfere with Mef2c or Mef2d function during heart formation, either Mef2c or Mef2d MOs were injected bilaterally into both dorso-vegetal blastomeres of eight-cell stage Xenopus embryos to target cardiac tissue [27].In all experiments, 0.5 ng GFP RNA was co-injected as a lineage tracer and to identify correctly injected embryos.Mef2c and Mef2d morphant embryos revealed abnormalities during cardiogenesis whereas Control MOinjected siblings showed a normal cardiac development (Fig. 2 A-D).Embryos depleted of Mef2c or Mef2d developed pericardial edema and malformed hearts and additionally showed a reduced heart rate at stage 42 (Fig. 2A, B).To take a closer look at morphant hearts, we isolated the hearts of fixed Xenopus embryos.We observed that the heart tube was formed and the looping process was initiated in both Mef2c and Mef2d morphant embryos.However, during later development, defects during the looping process as well as smaller cardiac chambers were observed in Mef2c and Mef2d MO injected embryos (Figure 2 A-D).These results indicate that both members of the Mef2 family are required for cardiogenesis in Xenopus, and are particularly important for cardiac morphogenesis and heart looping.

Mef2c or Mef2d are required for cardiac gene expression in the common cardiac progenitor cell population
Mef2 transcription factors have been shown to be important regulators of cardiac differentiation [14,16,17].To pinpoint the time point of when a loss of either Mef2 factor affects cardiogenesis, we analyzed the expression of different cardiac marker genes at different time points of cardiogenesis.For this purpose, we injected Mef2c or Mef2d MO unilaterally into one dorso-vegetal blastomere of eight-cell stage Xenopus embryos and analyzed marker gene expression at different stages by whole mount in situ hybridization.In this experimental setting, the uninjected side served as an internal control.The injected side was identified by co-injecting GFP RNA as a lineage tracer.We started our analysis at stage 20 when the CPC population is located on the ventral side of the embryo [8].At this stage, the expression of tbx20 was found to be down-regulated on the injected side of Mef2c or Mef2d-depleted embryos as compared to the uninjected side and Control MO-injected siblings (Fig. 3).Loss of Mef2d but not Mef2c reduced tbx1 expression at this stage.The expression of isl1 and nkx2-5 however was not affected in either Mef2c or Mef2d deficient embryos (Fig. 3).This observation indicates that both Mef2c and Mef2d are required for proper expression of cardiac genes in the Xenopus CPC population.
At stage 28, when the CPC population has split into the FHF and SHF [8], both Mef2c MO and Mef2d MO-injected embryos revealed reduced expression of FHF marker genes such as tbx20 and gata6b as well as SHF marker genes such as tbx1 and isl1.Marker genes indicating terminal differentiation of cardiomyocytes, namely actc1, myh6 and tnni3, were also down-regulated upon loss of either gene (Fig. 4).

The c domain of Mef2c is required for embryonic cardiogenesis
While cloning the Xenopus laevis mef2c gene from heart enriched explants, we isolated two different splice versions of this gene that differ in either the presence or absence of the c domain, mef2ccand mef2cc+, respectively.qPCR analyses with primer pairs specifically recognizing either splice variant revealed that both isoforms of Mef2c are expressed in heart tissue enriched explants cut at stages 24, 28, and 32 (Fig. 5 A).Please note that cardiac explants cannot be cut at stage 20 of development as the cardiac tissue at this stage is a thin layer of cells underlying the ectoderm in the anterior region of the embryo in close proximity to other sites of Mef2 expression.Interestingly, mef2ccwas expressed at higher levels on RNA level than the mef2cc+ variant.This is in agreement with previous data showing that mef2ccis the predominantly expressed variant during murine embryogenesis and the only isoform that was found to be expressed in the murine adult heart [12].Moreover, also mef2d could be detected by qPCR experiments.(Fig. 6A).
We next aimed to show the specificity of the observed phenotypes.To generate a mMef2ccconstruct, we deleted this domain in the mMef2cc+ construct.Western Blot experiments using a Mef2c antibody showed that both variants of mMef2c are expressed at similar levels upon RNA injection (Fig. 5 B).The same holds true for the injected hMEF2D RNA which was detected by a specific MEF2D antibody (Fig. 6B).For rescue experiments, we co-injected in a first set of experiments Mef2c MO together with RNA coding for murine Mef2cc+ and examined expression of myh6 and tnni3 at stage 28.This co-injection led to a significant rescue of marker gene expression (Fig. 5 C, D).Intriguingly, the mMef2ccversion failed to rescue the observed changes in gene expression upon Mef2c knock down (Fig. 5 E, F).These data indicate for the first time that the c domain of Mef2c has an important role during early cardiogenesis in Xenopus.The restored expression of myh6 and tnni3 was also observed in embryos co-injected with the Mef2d MO together with hMEF2D mRNA (Fig. 6 C, D).

Mef2d can compensate for the loss of mef2c but not vice versa
Given the similarities of the phenotypes observed upon depletion of either Mef2c or Mef2d but also taking into account the differences in the effects of either MO on gene expression in the CPC population, we aimed to further characterize the interplay of both factors.First, we injected low doses of Mef2c and Mef2d MO either alone or in combination into both dorsovegetal blastomeres of eight-cell stage embryos (Fig. 7 A, B).Interestingly, whereas neither a low dose of Mef2c MO nor Mef2d MO resulted in a phenotype, the co-injection of both MOs at this low dose together resulted in a robust phenotype in a more than additive manner indicating a synergistic activity of both transcription factors.We furthermore performed cross-rescue experiments by co-injecting Mef2c MO together with hMEF2D or Mef2d MO G-Browse).In all panels conserved genes are indicated by a color code.The orientation of the open reading frames of some genes is depicted by arrowheads.Gene length or distances between genes are not drawn to scale.A list of gene abbreviations used here is given in Table S1.doi:10.1371/journal.pone.0087294.g001with mMef2cc or mMef2cc ˜RNA and analyzed marker gene expression at stages 20 and 28.By co-injecting the Mef2c MO together with hMEF2D, the expression of tbx20 at stage 20 and bmp4, myh6 and tnni3 at stage 28 was significantly restored (Fig. 7  C, D).However, co-injection of Mef2d MO with mMef2c failed to rescue the observed phenotypes independent of the mMef2c splice variant used (Fig. 7 E-H).These data clearly indicate that Mef2d can compensate for a loss of Mef2c but not vice versa.
Overexpression of mMef2c+ or hMEF2D but not mMef2cc-accelerates the onset of cardiac differentiation Finally, we performed gain of function experiments by injecting RNA coding for either Mef2 variant unilaterally into eight-cell stage embryos.In a first set of experiments we examined the expression of tnni3 at stage 24 when expression of this gene commences.Injection of mMef2ccc or hMEF2D RNA resulted in a slight increase of expression on the injected side.This was not observed in case of RNA coding for mMef2cc-(Fig.8 A, B).In contrast, we did not see any significant differences between the injected and the uninjected side when analyzing tnni3 expression at stage 28 (Fig. 8 C, D).Taken together, these data indicate that overexpression of mMef2cc+ or hMEF2D but not mMef2ccrather accelerates the onset of cardiac differentiation than resulting in ectopic cardiomyocyte formation.

Discussion
Members of the Mef2 family of transcription factors are critical regulators of cardiac development in different species.However, no functional studies on mef2 genes during cardiogenesis have been performed in Xenopus laevis so far.A detailed synteny analysis as well as a comprehensive comparison of cDNA sequences confirmed the correct assignment of mef2a, c, and d homologs in Xenopus.This correct assignment is certainly important for the interpretation of the cross species rescue experiments performed in this study.Surprisingly, we were not able to identify any genomic sequence or any EST coding for mef2b in Xenopus and it is very likely that mef2b does not exist in Xenopus.It is possible, however, that due to the lack of sequence information available in current public sequence databases, the assembly of the corresponding scaffold might not be complete yet.We argue, however, that as long as no mef2b ortholog is identified in Xenopus, it is likely that this gene was lost during evolution in this species.
In mice, Mef2a and Mef2c are functionally important for cardiac development as assessed by the phenotypes in genetically modified mice that lack either of these genes [17,18].Knock out mice of either Mef2a or Mef2c die during embryogenesis due to problems in cardiac development.In contrast, Mef2d null mice develop normally until birth.Interestingly, mef2a expression was recently found not to be present during cardiac development in Xenopus [13], whereas mef2c and mef2d were robustly expressed during cardiogenesis [8].Our functional analyses performed in this study indicate that indeed both members are needed for proper heart formation in Xenopus.Moreover, based on our results, we speculate that in Xenopus, Mef2d has taken over the function that Mef2a has in mice.A more detailed analysis of marker genes of Mef2a-deficient mice might shed more light onto this evolutionary difference.In addition, the identification of genomic regions bound by Mef2a in mice and Mef2d in Xenopus might help to verify or falsify this hypothesis.
Of interest is also the observation that loss of Mef2c can be rescued by an overexpression of Mef2d but not vice versa.Mef2 transcription factors have been shown to form homo-and heterodimers [9,10].It is therefore very likely that Mef2c/Mef2d and Mef2d/Mef2d dimers or Mef2d monomers are functionally important during early cardiac development in Xenopus.In this scenario, Mef2c/Mef2c dimers, or Mef2c monomers, would not be similarly efficient in transcription.Our findings therefore should foster more detailed analysis of this issue not only in Xenopus but in mouse as well.
Although Mef2a and Mef2c knock out mice have a cardiac phenotype, the role of both genes in regulating cardiac gene expression in the CPC population has not been reported and also the expression of Mef2 proteins in the early embryo has not yet been described.However, Mef2c has been shown to be expressed in early differentiating murine ES cell cultures suggesting a possible role for Mef2c in the CPC population [28].In line with these findings is the observation that the use of a dominant negative Mef2 protein that blocks the activity of all four members of the Mef2 family in mice inhibits cardiac differentiation in P19 cells [29].These data clearly indicate a functional redundancy of Mef2 members in mice.Interestingly, the loss of Mef2c is additionally accompanied by an up-regulation of Mef2b.Our data shown here clearly indicate that the loss of either Mef2c or Mef2d results in early defects during Xenopus cardiac differentiation thus suggesting that this functional redundancy is not so prominent in Xenopus.This statement is also supported by the observation of a more than additive phenotype after simultaneously knocking down both Mef2c and Mef2d, which thereby implies that these two transcription factors are acting synergistically rather than redundantly.The apparent lack of Mef2b in Xenopus might contribute to this disparate situation as well.
Transcriptional targets of Mef2 factors that have been identified in different species include alpha cardiac myosin heavy chain, alpha cardiac actin and cardiac troponin I [30][31][32].Notably, the expression of these genes was down-regulated in our experiments after knocking down the mef2 genes (see Fig. 4, myh6, actc1 and tnni3,) suggesting that their regulation by Mef2 factors is also conserved in Xenopus.In the case of myh6, a recent study identified a conserved Mef2 binding site in its promoter region [33].Altogether, the loss and down-regulation of marker gene expression in our morphant embryos is clearly in line with what previous studies have shown.Of particular interest as a target of Mef2 factors is Nkx2-5 [29].Interestingly, we have not observed any down-regulation of nkx2-5 expression in the CPC population at stage 20, but we have seen a down-regulation at the stage 28.This might suggest that nkx2-5 is a target gene of Mef2 proteins only at later stages of development or this observation might point to diverse functions of Mef2 proteins as discussed in the next section.
The c domain of mMef2c has been shown to function as a transcriptional repressor [12].Our rescue experiments suggest that Mef2cc+ but not Mef2cc-is required for early cardiogenesis.This finding is unexpected because we found mef2c-to be expressed higher on RNA level than the mef2c+ isoform.Also in adult muscle and during skeletal myogenesis the c version has been described to be predominantly expressed and to be of functional relevance [12].Of note, we cannot exclude other functions for the mef2cc-variant that were not tested in our study.Interestingly, overexpression of a Mef2 engrailed repressor fusion protein (MEF2/EnR) in P19 cells results in an activation of Nkx2-5 during early phases of cardiogenesis whereas this construct inhibits  Mef2c MO was unilaterally injected along with RNA coding for hMEF2D.Expression of cardiac marker genes was monitored at stages 20 (tbx20, bmp4; anterior views) or stage 28 (myh6, tnni3; ventral views), respectively.E, F. Mef2d MO was unilaterally injected along with mMef2cc+ RNA.Expression of cardiac marker genes was monitored at stages 20 (tbx1, tbx20; anterior views) or cardiogenesis at later time points [29].This is in line with our findings obtained here and implies that different splice variants of Mef2 transcription factors might be expressed during different phases of cardiac development.This has not been analyzed, at least to our knowledge, in a time and cell type specific manner during vertebrate cardiogenesis.The functional differences of two of these splice variants in our study indicate the requirement of additional expression and functional studies in the future to complete our understanding of Mef2 function during cardiogenesis.The c domain has also been shown to be subject to regulation by phosphorylation [12] suggesting that this type of regulation occurs during early cardiac development adding another layer of complexity to Mef2 function during this time period.The fact that human MEF2D that also harbors a sequence similar to the c domain of Mef2c is able to rescue the Mef2c MO phenotype might trigger further experiments to analyze this short stretch of amino acids in MEF2D in more detail.Finally, the discovery that Mef2d is involved in Xenopus cardiogenesis may also be useful as another model suitable to gain further insights into the function of Mef2d during cardiac remodeling in mice, particularly in the reactivation of a fetal gene expression program.
Of note, our experiments have additional implications as Mef2c was used as one of three components in direct reprogramming experiments to generate cardiomyocytes out of cardiac fibroblasts [25].Our findings should therefore also foster research that examines different Mef2 splice variants and their potential roles and functions in this setting with the aim to increase the efficiency of reprogramming.

Xenopus embryos
Xenopus laevis embryos were obtained by in vitro fertilization, cultured and staged according to Nieuwkoop and Faber [34].All procedures were performed according to the German animal use and care law (Tierschutzgesetz) and approved by the German state administration Baden-Wu ¨rttemberg (Regierungspra ¨sidium Tu ¨bingen).

Cloning
The mMef2c and hMEF2D constructs were purchased from ImaGenes GmbH and the open reading frames were subcloned into pCS2+ [35].To obtain a rescue construct which is not targeted by the Mef2d MO, we inserted two additional silent point mutations in the D59UTR hMEF2D by mutagenesis using the QuickChange II Site-Directed Mutagenesis Kit (Stratagene).All constructs were verified by sequencing.The mMef2ccdeletion construct was generated by inverse PCR using the mMef2cc+/ pCS2+ rescue construct as template and the proof reading Phusion DNA polymerase (Finnzyme) followed by re-ligation.The primers used for the inverse PCR were: for: 59-GAC CGT ACC ACC ACC CCT TCG A-39; rev: 59-GCT GAG GCT TTG AGT AGA AGG CAG G-39.For cloning full length mef2c from heart enriched explants the following primers were used: Mef2c cloning for: 59-GTT GGA GCA GAG GGG AAA AT-39, Mef2c cloning rev: 59-GGT ATA AGC ACA CAC ACA CTG CA-39.

Morpholino oligonucleotide (MO), MO specificity tests and RNA injections
All MOs were purchased from Gene Tools, LLC, OR USA, resuspended in DEPC-H 2 O and stored as aliquots at 220uC.Mef2cMO: 59-CCA TAG TCC CCG TTT TTC TGT CTT C-39; Mef2dMO: 9-AAT CTG GAT CTT TTT TCT GCC CAT G-395.For knock down approaches, we injected the MOs (10 ng) into both dorso-vegetal blastomeres of eight-cell embryos to target the presumptive heart region [27].For lineage labeling and to identify the injected side, we co-injected 0.5 ng GFP RNA.Only correctly injected embryos were considered for the experiments.For control injection experiments, the standard Control MO of Gene Tools was used.Control MO: 59-CCT CTT ACC TCA GTT ACA ATT TAT A-39 For rescue experiments, mRNA (0.5 ng) was injected together with MO.The binding specificity of MOs was tested in vivo were cloned in frame with and in front of the GFP open reading frame in pCS2+.The indicated RNA and MO were co-injected bilaterally into two-cell stage embryos and GFP translation was monitored at stage 20 using a fluorescence microscope.

Whole Mount in situ Hybridization (WMISH)
Probes for mef2c and mef2d were used as previously described [8].Digoxigenin-labelled antisense RNA probes of analyzed genes were synthesized by restriction digestion and subsequent transcription with Sp6 or T7 RNA polymerase (Roche).To analyze marker gene expression at stages 20 and 28, MOs (10 ng) were unilaterally injected in one dorso-vegetal blastomere together with 0.5 ng GFP RNA.The uninjected side served as internal control.Wild type or injected embryos were fixed at +4uC in MEMFA (0.1 M MOPS, pH 7.4, 2 mM EGTA, 1 mM MgSO4, and 4% formaldehyde) at indicated stages.WMISH experiments were performed according to a standard protocol as previously described [36,37].BM-Purple (Roche) was used for staining.Stained embryos were then bleached with 30% H 2 O 2 .For sections, stained embryos were embedded in gelatine/albumine overnight at +4uC, sectioned using a vibratome with a thickness of 25 mm, coverslipped, and imaged with an Olympus BX60 microscope.
Western Blot 30 embryos injected with RNA were homogenized in 300 ml lysis buffer (20 mM Tris/HCl, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 1 mM PMSF) and incubated on ice for 5 min.Protein samples were cleared at 13,000 rpm at 4uC for 5 min.For removal of lipids, the supernatant was mixed with 50 ml Freon followed by centrifugation at 13,000 rpm at 4uC for 5 min.The upper protein containing phase was collected.Concentration of protein samples was determined by Bradford assay with BSA as standard.Western blotting was performed according to standard procedures and proteins were visualized using a Li-COR ODYSSEY Imager.Primary antibodies were purchased from Abcam (rabbit polyclonal Mef2C, directed against residues 450 to the C-terminus of human MEF2C), Santa Cruz (mouse monoclonal anti Mef2D, H11, epitope mapping within an internal region of MEF-2D of human origin) and Serotec (c-Tubulin, clone YL1/2).Secondary antibodies used were IRDye conjugates from Li-COR.

Synteny analysis
For synteny analysis, genomic structure and chromosomal organization of Mef2a, b, c and d in human, mouse and X. tropicalis were compared using NCBI and Xenbase G Browse.

Statistics
Data were obtained from at least three independent experiments and analyzed with statistical program GraphPad Prism.The number of embryos (N) and the number of independent experiments (n) performed for each experiment is indicated in the corresponding figures.For rescue experiments, embryos of the same batch were evaluated upon injection either with either MO or MO along with the RNA of interest.The nonparametric Mann-Whitney rank sum test was used to determine statistical differences.A p value of #0.05 was considered to be significant.

Figure 2 .
Figure 2. Depletion of either mef2c or mef2d leads to severe cardiac defects.A, C. Mef2c-or Mef2d MO-injected embryos developed cardiac edema and morphological heart defects at stage 42, as compared with Control MO-injected sibling embryos.The amount of MO injected in total (two blastomeres) is given in ng.In addition, cardiac beating was reduced upon Mef2c or Mef2d MO depletion.The dotted dark gray lines indicate the heart.a: atria; v: ventricle; oft: outflow tract.B, D. Quantitative presentations are shown.N: number of examined embryos; n: number of independent experiments.doi:10.1371/journal.pone.0087294.g002

Figure 3 .
Figure 3. Loss of Mef2c or Mef2d affects the cardiac progenitor cell population.Mef2c or Mef2d MO (10 ng) was unilaterally injected and expression of cardiac marker genes was monitored at stage 20. A. tbx20 expression was down-regulated in Mef2c MO-or Mef2d MO-injected embryos (arrowheads).In addition, Mef2d-but not Mef2c-depleted embryos showed reduced tbx1 expression (arrowhead).Expression of isl1 as well as bmp4 remained unchanged upon loss of Mef2c or Mef2d.Anterior views of embryos are shown.B. Quantitative presentation of observed phenotypes in A is given.N: number of examined embryos; n: number of independent experiments; *, p#0.05.doi:10.1371/journal.pone.0087294.g003

Figure 5 .Figure 6 .
Figure 5. Specificity of phenotype observed upon knock down of Mef2c. A. mef2cc-and mef2cc+ isoforms are expressed on RNA level in heart tissue enriched explants at stages 24, 28, and 32 as revealed by qPCR.Expression is shown relative to gapdh.B. mMef2cc-and mMef2cc+ are expressed on protein level upon RNA injection into Xenopus embryo at comparable levels.b-Tubulin served as loading control.Note that the Mef2c antibody used does not recognize endogenous Xenopus Mef2c protein.The asterisk indicates unspecific background.C-F.Mef2c MO was unilaterally injected together with GFP, mMef2cc+ or mMef2c-RNA as indicated.C, E Expression of the cardiac marker genes myh6 and tnni3 was monitored at stage 20 or stage 28.Black arrowheads indicate reduced marker gene expression, white arrowheads highlight the rescued situation.Ventral views of embryos are shown.D, F. Quantitative presentations are shown.N: number of examined embryos; n: number of independent experiments; st: stage; **, p#0.01.doi:10.1371/journal.pone.0087294.g005

Figure 7 .
Figure 7. Cooperation of Mef2c and Mef2d in Xenopus.A, B. The injection of 7 ng Mef2c MO or 7 ng Mef2d MO (in both cases 3.5 ng per blastomere) did not result in cardiac defects.The co-injection of 7 ng Mef2c together with 7 ng Mef2d MO led to a significant increase of the phenotype.The dotted black lines indicate the heart.C, D. Mef2c MO was unilaterally injected along with RNA coding for hMEF2D.Expression of cardiac marker genes was monitored at stages 20 (tbx20, bmp4; anterior views) or stage 28 (myh6, tnni3; ventral views), respectively.E, F. Mef2d MO was unilaterally injected along with mMef2cc+ RNA.Expression of cardiac marker genes was monitored at stages 20 (tbx1, tbx20; anterior views) or

stage 28 (Figure 8 .
Figure 8. Gain of function analyzes reveals an earlier onset of cardiac differentiation.A, C. RNA coding for mMef2cc+, mMef2cc-or hMEF2D was injected unilaterally into the dorsal-vegetal blastomere at eight cell stage and tnni3 expression was monitored at stages 24 (A) or 28 (C).Ventral views of embryos are shown.B, D. The percentage of embryos with enhanced expression of tnni3 on the injected side is given.N: number of examined embryos; n: number of independent experiments; **, p#0.01.doi:10.1371/journal.pone.0087294.g008

Figure
Figure S1 Spatio-temporal expression of mef2c and mef2d in Xenopus. A. Temporal expression of mef2c.mef2c is maternally supplied.Mef2c embryonic expression starts at stage 9 and increases until stage 40.gapdh was used as loading control.-RT serves as negative control.B. Temporal expression of mef2d.mef2d is maternally supplied.Mef2d embryonic expression starts at stage 12. H4 was used as loading control.-RT serves as negative control.C-j.Spatial expression of mef2c.C. Anterior view with the dorsal side to the top.c.Sagittal section.D, F, H, J. Lateral views with anterior to the right.E, G, I, K. Ventral views with anterior to the top.Black arrowheads indicate the expression in the FHF, the red arrowhead highlights mef2c transcripts at the lateral sides of the SHF.C. Parasagittal section.d, f, h, j.Transverse sections.Black arrowheads indicate cardiac expression; the arrowhead in h