Differentiation of the Emerging Human Pathogens Trichosporon asahii and Trichosporon asteroides from Other Pathogenic Yeasts and Moulds by Using Species-Specific Monoclonal Antibodies

The fungal genus Trichosporon contains emerging opportunistic pathogens of humans, and is the third most commonly isolated non-candidal yeast from humans. Trichosporon asahii and T. asteroides are the most important species causing disseminated disease in immunocompromised patients, while inhalation of T. asahii spores is the most important cause of summer-type hypersensitivity pneumonitis in healthy individuals. Trichosporonosis is misdiagnosed as candidiasis or cryptococcosis due to a lack of awareness and the ambiguity of diagnostic tests for these pathogens. In this study, hybridoma technology was used to produce two murine monoclonal antibodies (MAbs), CA7 and TH1, for detection and differentiation of Trichosporon from other human pathogenic yeasts and moulds. The MAbs react with extracellular antigens from T. asahii and T. asteroides, but do not recognise other related Trichosporon spp., or unrelated pathogenic yeasts and moulds including Candida, Cryptococcus, Aspergillus, Fusarium, and Scedosporium spp., or the etiologic agents of mucormycosis. Immunofluorescence and Western blotting studies show that MAb CA7, an immunoglobulin G1 (IgG1), binds to a major 60 kDa glycoprotein antigen produced on the surface of hyphae, while TH1, an immunoglobulin M (IgM), binds to an antigen produced on the surface of conidia. The MAbs were used in combination with a standard mycological growth medium (Sabouraud Dextrose Agar) to develop an enzyme-linked immunosorbent assay (ELISA) for differentiation of T. asahii from Candida albicans and Cryptococcus neoformans in single and mixed species cultures. The MAbs represent a major advance in the identification of T. asahii and T. asteroides using standard mycological identification methods.


Introduction
The genus Trichosporon contains approximately 50 species of basidiomycete yeasts found in a wide variety of habitats including soil and indoor environments [1][2][3]. A number of species colonise the skin, gastrointestinal, respiratory and urinary tracts of humans [2,3], and superficial Trichosporon infections of immunocompetent individuals such as white piedra infections of the hair shaft are well documented [2,3]. As well as superficial infections, repeated inhalation of Trichosporon arthroconidia can cause summer-type hypersensitivity pneumonitis (SHP) [4,5], an immunologically induced lung disease. It is the most common form of hypersensitivity pneumonitis (HP) in Japan [4] and T. asahii is the most frequent cause of the disease [1,3]. However, other fungi such as the basidiomycete yeast Cryptococcus may be responsible for SHP [6] and accurate identification of the causative species is necessary for appropriate treatment, which is challenging due to the number of different techniques required [7][8][9][10].
Certain Trichosporon species have also emerged as rare but frequently fatal pathogens causing disseminated infections (trichosporonosis) in immunocompromised individuals and ICU patients [2,3,[11][12][13][14][15][16][17][18]. The genus Trichosporon was the third most commonly isolated non-candidal yeast from clinical specimens in the ARTEMIS DISK global antifungal surveillance study [14] and is the second most common cause, after Candida species, of yeast fungaemia in patients with haematological malignancies [2,3]. High mortality rates are associated with trichosporonosis, with reports in the literature of between 50 and 80% in high-risk patient groups [2].
Early diagnosis of invasive trichosporonosis is critical for prompt and effective treatment [2,3] but this is difficult for Trichosporon infections [17], especially in resource-limited settings with rudimentary diagnostic facilities. Currently, for a proven diagnosis of invasive trichosporonosis, the fungus must be cultured from biopsies [3,17], but obtaining biopsy samples from critically ill patients is problematic. Furthermore, Trichosporon asahii, the most common cause of disseminated disease, may be mistaken for Candida spp. in culture, especially where mixed yeast or mould species are recovered [7,19,20], and methods for identifying Trichosporon to species level, based on morphological characteristics and biochemical profiling are time consuming, require specialist training, appropriately equipped laboratory facilities, and can yield inconsistent results [7]. Nucleic acid-based methods are necessary to distinguish Trichosporon spp., but no such methods have been standardised for clinical use and no commercially available tests currently accommodate the revised taxonomic classification of the genus [8][9][10]21]. Cross-reactivity of commercial immunoassays with Trichosporon, such as the Cryptococcus antigen test [22], can also lead to mis-identification and inappropriate use of antifungal drugs.
Accurate and relatively simple diagnostic procedures are needed for Trichosporon detection [2,3,17]. Hybridoma technology allows the generation of highly specific monoclonal antibodies (MAbs) [23][24][25] that can be used to develop rapid and highly accurate immunodiagnostic tests capable of differentiating fungal pathogens to genus-, species-or even isolate-level [23][24][25]. This paper describes the use of hybridoma technology to develop two murine hybridoma cell lines (CA7 and TH1) producing MAbs specific for Trichosporon asahii and the closely related species Trichosporon asteroides, the most important causes of trichosporonosis. The accuracy of the MAbs in differentiating T. asahii from other yeast pathogens in mixed cultures is demonstrated using a highly specific ELISA combined with a standard mycological culture method.

Ethics Statement
All animal work described in this study was conducted under a UK Home Office Project License, and was reviewed by the institution's Animal Welfare Ethical Review Board (AWERB) for approval. The work was carried out in accordance with The Animals (Scientific Procedures) Act 1986 Directive 2010/63/EU, and followed all the Codes of Practice which reinforce this law, including all elements of housing, care, and euthanasia of the animals.

Preparation of Immunogen, Immunisation Regime, and Animal Welfare
For preparation of the immunogen, flasks containing 100 ml of sterile MYB were inoculated with 10 3 spores of T. asahii ml 21 of medium. The flasks were incubated at 26uC with shaking (125 rpm) for 2 d, after which the contents were centrifuged at 4000 rpm for 5 min, the bulk of the supernatant was discarded and the pelleted cells re-suspended in 10 ml of remaining culture fluid. The surface of 2-day-old MYA petri-dish cultures were scraped and combined with the re-suspended cells. The combined cell preparations were snap frozen in liquid nitrogen, lyophilized for 3 d and the dried material stored at 220uC prior to use. Before immunisation, the immunogen was reconstituted with phosphate buffer saline (PBS; 137 mM NaCl, 2.7 mM KCl, 8 mM Na 2 HPO 4 , and 1.5 mM KH 2 PO 4 [pH 7.2]) to make a cell suspension containing 10 mg biomass ml 21 buffer. For immunisations, 6-week-old BALB/c white mice were each given four intraperitoneal injections (300 ml per injection) of immunogen at 2-week intervals and a single booster injection was given five days before fusion.

Production and Screening of Hybridomas and Determination of Antibody Specificities
Hybridoma cells were produced by the method described elsewhere [24,25] and the supernatants were screened by enzymelinked immunosorbent assay (ELISA) against antigens immobilized to the wells of Maxisorp microtitre plates (442404; Nunc) (50 ml per well). For antibody specificity tests, antibodies were tested against surface washings [25] prepared from replicate slant cultures of fungi. Protein concentrations, determined spectrophotometrically at 280 nm (Nanodrop, Agilent Technologies Limited, Berkshire, UK), were adjusted with PBS to produce equivalent protein concentrations for each organism. Fifty ml volumes were then used to coat the wells of microtitre plates. After incubating overnight at 4uC, wells were washed four times with PBST (PBS containing 0.05% Tween-20) and once each with PBS and dH 2 O and air-dried at 23uC in a laminar flow hood. The plates were stored in sealed plastic bags at 4uC in preparation for screening of hybridoma supernatants by ELISA.

Enzyme-Linked Immunosorbent Assay
Wells containing immobilised antigens were blocked for 15 min with 100 ml of PBS containing 1% Bovine Serum Albumin (BSA) and, after one 5-min wash with PBS, were incubated successively with hybridoma supernatant for 1 h, followed with goat antimouse polyvalent (immunoglobulin classes IgG, IgA, and IgM) peroxidase conjugate (A-0412; Sigma Chemical Company, Poole, United Kingdom) diluted 1 in 1000 in PBST for a further hour. Bound antibody was visualized by incubating wells with tetramethyl benzidine (T-2885; Sigma) substrate solution [24,25] for 30 min. The reactions were stopped by the addition of 3 M H 2 SO 4 . Absorbance values were determined at 450 nm with an automated microplate reader (iMark TM microplate reader MPM6, BIORAD, Hertfordshire, UK). Wells were given four 5-min rinses with PBST between incubations. Working volumes were 50 ml per well, and control wells were incubated with tissue culture medium (TCM) containing 10% fetal bovine serum. All incubation steps were performed at 23uC in sealed plastic bags. The threshold for detection of the antigen in ELISA was determined from control means (26TCM absorbance values) [25]. These values were consistently in the range 0.050-0.100. Consequently, absorbance values .0.100 were considered as positive for the detection of antigen.

Determination of Ig Subclass and Cloning Procedure
The Ig class of MAbs was determined by using antigenmediated ELISA. Wells of antigen-coated microtitre plates were incubated successively with hybridoma supernatant for 1 h, followed with goat anti-mouse IgG 1 , IgG 2a , IgG 2b , IgG 3 , IgM, or IgA-specific antiserum (ISO-2; Sigma) diluted 1 in 3000 in PBST for 30 min and rabbit anti-goat peroxidase conjugate diluted 1 in 1000 (A-5420; Sigma) for a further 30 min. Bound antibody was visualized with TMB substrate as described. Hybridoma cells lines were sub-cloned three times by limiting dilution, and cell lines were grown in bulk in a non-selective medium preserved by slowly freezing in foetal bovine serum/ dimethyl sulfoxide (92:8), and stored in liquid nitrogen.

Antigen Characterisation by Heat Treatment, Periodate Oxidation and Protease Digestion
Heat stability studies were conducted by placing tubes of solubilised antigen from three replicate cultures of the fungus in a boiling water bath. At 10 min intervals, samples were removed, centrifuged at 14,500 rpm for 5 min, and antigens immobilised to the wells of microtitre plates for assay by ELISA as described. For periodate oxidation, microtitre wells containing immobilised antigens were incubated with 50 ml of sodium meta-periodate solution (20 mM NaIO 4 in 50 mM sodium acetate buffer [pH4.5]) or acetate buffer only (control) at 4uC in sealed plastic bags. Plates were given four 3-min PBS washes before processing by ELISA as described. For protease digestions, microtitre wells containing immobilised antigen were incubated with 50 ml of pronase (protease XIV; 9 mg ml 21 in PBS) or trypsin (1 mg ml 21 in Milli-Q H 2 O) solution or Milli-Q H 2 O or PBS only (controls) for 4 h at 37uC or 4uC. Plates were given four 3-min rinses with PBS and then assayed by ELISA as described.
Protease digestion studies were also conducted using DOT-BLOTS of antigen immobilised to polyvinylidene difluoride (PVDF) membranes. Membranes were incubated in pronase or trypsin solutions or controls for 4 h at 37uC with shaking (25 rpm). Membranes were washed three times with PBS and then blocked for 16 h at 4uC, with PBS containing 1% BSA. Blocked membranes were incubated with MAb supernatant diluted 1 in 2 with PBS containing 0.5% BSA (PBSA), for 2 h at 23uC. After washing three times with PBS, membranes were incubated for 1 h with goat anti-mouse alkaline phosphatase conjugate, diluted 1 in 15,000 in PBSA (either IgM m-chain specific, Sigma; A9688 or IgG whole molecule, Sigma; A3562). Membranes were washed three times with PBS, once with PBST and bound antibody visualised by incubation in substrate solution [24,25]. Reactions were stopped by immersing membranes in dH 2 O, and membranes were then air dried between sheets of Whatman filter paper.

Polyacrylamide Gel Electrophoresis and Western Blotting
SDS-PAGE was carried out using 4-20% gradient polyacrylamide gels under denaturing conditions. Surface washings of T. asahii var. asahii CBS8972, T. asteroides CBS6183 and T. inkin CBS7630 cultures grown on MYA slants were prepared every 24 h following inoculation, using 3 ml of sterile Milli-Q water. Washings were centrifuged for 5 min at 14,500 rpm to precipitate cells and hyphae and the supernatants containing soluble antigens were denatured by mixing with Laemmli buffer and heating at 95uC for 10 min. Proteins were separated electrophoretically at 165 V and pre-stained, broad-range markers (Bio-Rad Laboratories Limited, Hemel Hempstead, UK) were used for molecular weight determinations. For Western blotting, separated proteins were transferred electrophoretically on to a PVDF membrane for 2 h at 75 V. Thereafter, the membranes were processed with MAb supernatants according to the procedure used for DOT-BLOTS.  Immunofluorescence Sterilised slides were coated with a washed yeast cell suspension containing 1% glucose and incubated at 26uC for 16 h. After airdrying, the slides were fixed and incubated with hybridoma supernatant for 1 h, followed by three 5 min PBS washes. Slides were then incubated with goat anti-mouse polyvalent fluorescein isothiocyanate conjugate (diluted 1 in 40 in PBS) (Sigma; F1010) for 30 min. Slides were given three 5 min washes with PBS and mounted in PBS-glycerol mounting medium before overlaying with coverslips. All incubation steps were performed at 23uC in a humid environment to prevent evaporation and slides were stored in the dark, at 4uC, prior to examination using an epifluorescence microscope (Olympus IX81).

Differentiation of Trichosporon from Candida and Cryptococcus in Mixed Species Cultures
Petri dish culture plates containing SDA were inoculated with cell suspensions of T. asahii, C. albicans or C. neoformans, either as single species cultures or as species mixtures. After 24 h incubation at 26uC, antigen solutions were prepared by flooding the plates with 10 ml PBS, suspending cells using sterile L-shaped spreaders, and pelleting of cells by centrifugation at 14,500 rpm for 5 min. Protein concentrations of solutions were adjusted to 60 mg ml 21 and used to coat the wells of microtitre plates for assay by ELISA.

Data Analysis
Differences in means were analysed by one-way analysis of variance (ANOVA) and Tukey-Kramer tests were used to determine statistical significance.

Production of Hybridoma Cell Lines and Isotyping of MAbs
Two fusions were performed and 1284 hybridoma cell lines were screened for MAb production by PTA-ELISA. Two of the MAbs (CA7 and TH1) were selected for further testing on the basis of their high absorbance values (A 450 0.400 and A 450 1.122 respectively). Isotyping of the MAbs showed that CA7 belongs to immunoglobulin class G1 (IgG1) and TH1 to immunoglobulin class M (IgM).

MAb Specificity Tests
In ELISA specificity tests, using antigen protein concentrations of 60 mg ml 21 , MAbs CA7 and TH1 reacted against surface antigens from T. asahii and T. asteroides, but did not react with surface antigens from other Trichosporon species or a broad range of clinically relevant yeasts and moulds including Candida, Cryptococcus and Aspergillus species (Table 1). While MAb CA7 failed to recognise T. asahii var. asahii CBS 5286 at this protein concentration, MAb TH1 gave a positive absorbance value (Abs 450 .0.100) for this strain. Consequently, when used together at this protein concentration, the MAbs gave a combined positive reaction with all of the T. asahii and T. asteroides strains tested (Figs. 1A and 1B). When tested against higher concentrations of protein (.60 mg ml 21 ), both MAbs reacted strongly with T. asahii CBS 5286 (Figs. 1C and 1D), while concomitant detection of other Trichosporon species (T. mucoides and T. inkin), and the unrelated pathogens Candida and Cryptococcus did not occur at equivalent concentrations of protein (Figs. 1C and 1D). Despite this, TH1 detection of T. asahii 8973, T. asahii 7632, T. asahii 5599, and T. asteroides strains 7623 and 7624 did not occur at the higher concentrations of protein, while MAb CA7 detected all five of these strains at protein concentrations $60 mg ml 21 . Consequently, the use of both antibodies at protein concentrations in the range 60 mg ml 21 to 480 mg ml 21 would allow for unequivocal detection of T. asahii and T. asteroides and differentiation from other infectious fungi.

Heat, Chemical and Enzymatic Characterisation of Antigens
The T. asahii var. asahii CBS8972 antigens were subjected to different treatments including heat ( Fig. 2A) and chemical and enzymatic modifications (Tables 2 & 3). Reductions in MAb binding in ELISA following heating show that an epitope is heat labile. Reductions in binding following treatment with pronase show that the epitope consists of protein, while reductions with trypsin show a protein epitope contains positively charged lysine and arginine side chains. Reductions in antibody binding following chemical digestion of an antigen with periodate shows that its epitope is carbohydrate and consists of vicinal hydroxyl groups.
The epitope bound by MAb CA7 was heat stable, with significant (p,0.001) reductions in ELISA absorbance only occurring after 40 min of heating ( Fig. 2A). CA7 binding to its epitope was also significantly reduced (p,0.001) following periodate treatment for 4 and 23 h (Table 2), pronase treatment at 37uC and 4uC, and trypsin treatment at 37uC (Table 3) when compared to controls. Binding of TH1 to its target antigen was significantly (p,0.001) reduced following heat treatment for 10 min (Fig. 2A), periodate treatment for 23 h (Table 2) and pronase treatment at 37uC (Table 3). Trypsin treatment of the antigen did not significantly reduce TH1 binding (Table 3). DOT-BLOT studies of protease treated antigen showed similar results to ELISA tests. TH1 reactivity with antigen immobilised to PVDF membrane was reduced following treatment with pronase, but was unaffected by trypsin. CA7 reactivity was reduced by both proteases. The relative insensitivities of the MAbs to periodate treatment, but sensitivities to protease digestion in both ELISA and DOT-BLOT tests indicate that MAbs CA7 and TH1 bind to protein epitopes in heat stable and heat labile glycoprotein antigens respectively.

Western Blotting and Immunofluorescence
Monoclonal antibody CA7 reacted with immuno-reactive glycoprotein antigen(s) from T. asahii and T. asteroides, with molecular masses in the region 48 to 74 kDa, and with a major glycoprotein antigen of approximately 60 kDa (Fig. 2B). These immuno-reactive antigens were absent in T. inkin antigen extracts. Monoclonal antibody TH1 did not bind to immuno-reactive antigen(s) in Western blotting studies. Immunofluorescence studies  showed that MAb CA7 binding was specific to the surface of hyphae (Fig. 2D), whereas TH1 binding was specific to the surface of conidia (Fig. 2F).

Differentiation of Trichosporon from Candida and Cryptococcus in Mixed Species Cultures
Yeasts were cultured on SDA plates for 24 h (Fig. 3A) and antigen solutions tested by ELISA using both MAbs (Fig. 3B&C). Monoclonal antibodies CA7 (Fig. 3B) and TH1 (Fig. 3C) were highly accurate in detecting T. asahii both when grown in single culture, and when grown on plates containing mixed populations of C. albicans and C. neoformans. The MAbs did not cross react with C. albicans or C. neoformans, when grown either as single species or as mixed populations.

Discussion
This paper describes the production of two murine MAbs, CA7 and TH1, raised against surface antigens from Trichosporon asahii. Specificity tests showed that both MAbs reacted only with antigens from the species Trichosporon asahii and Trichosporon asteroides. When combined in an ELISA, the MAbs were able to detect all of the isolates of T. asahii and T. asteroides tested. The MAbs did not crossreact with other clinically important yeasts and moulds, demonstrating their exquisite specificity.
Trichosporon asahii is the principal etiologic agent of trichosporonosis in humans [2,3]. While T. asteroides was originally described as a pathogenic agent of superficial infections [26], its prevalence as an agent of disseminated infection is increasing, since the first recorded case of T. asteroides trichosporonosis in 2002 [26]. Currently, the species is reported to be second only to T. asahii as the cause of trichosporonosis in immunocompromised patients [2,3]. Consequently, MAbs CA7 and TH1 represent useful diagnostic reagents for the immunological detection of the most important agents of this disease.
Recognition of these two species, but lack of recognition of the other Trichosporon species tested, may be explained by their close phylogenetic relatedness. Phylogenetic studies of the Trichosporon genus have shown that Trichosporon asahii and Trichosporon asteroides belong to a distinct clade, clade ovoides [21]. Comparisons of their ITS (internal transcribed spacer) regions show 98.7% similarity and for the intergenic spacer 1 (IGS1) region, 75.1% similarity [21]. When the mitochondrial cytochrome b genes of the species are compared, DNA sequences are synonymous and differ by only 4.0%, meaning that the two species contain proteins with identical amino acid sequences [27].
Incorrect diagnosis of invasive trichosporonosis as candidiasis or cryptococcosis is a critical factor in the successful treatment of infected patients [3], since these fungi differ in their susceptibilities to the different classes of antifungal drugs used to treat human mycoses [3,14,17,18,21,28,29]. Cross-reactivity of the cryptococcal latex agglutination test with Trichosporon species can lead to incorrect identification and inappropriate treatment [22]. Trichosporon and Cryptococcus both secrete glucuronoxylomannans (GXM) and common domains are shared between the polysaccharides of the two species, forming antigens detected by the test [22]. Cryptococcal meningitis is recognised as an AIDS defining illness and the most common cause of adult meningitis [30]. Although Cryptococcus neoformans is the species most frequently detected in meningitis patients, other yeasts are isolated, including emergent human pathogens such as Trichosporon and Rhodotorula [2,30]. Unlike the MAb used in the cryptococcal diagnostic test, MAbs CA7 and TH1 did not cross-react with other yeast species including C. neoformans and Rhodotorula, and so eliminate any ambiguity in species identification.
Western blotting studies showed that CA7 binds to a major glycoprotein antigen with a molecular weight of approximately 60 KDa, while immunofluorescence tests showed that the MAbs recognise surface antigens present on different morphological structures of Trichosporon asahii. Binding of MAb CA7 was specific to antigen(s) present on the surface of hyphae, whereas TH1 binding was specific to the surface of conidia. When combined, the MAbs are able to recognise both yeast and filamentous forms of the fungus, an important characteristic given that both of these stages of the pathogen are implicated in tissue invasion in disseminated infections [2,3]. The different morphologies have also been seen in the formation of plaques [18] which are an important risk factor associated with invasive trichosporonosis and, when present on devices such as catheters, allow the fungus to evade treatment with yeast active drugs.
The increasing prevalence of patients with mixed yeast infections, particularly polymicrobial ones [7,19,20,30], and problems of identifying the causative agents in mixed species cultures motivated us to test the accuracy of the MAbs in detecting Trichosporon in mixed populations with Candida and Cryptococcus. Diagnostic laboratories often favour the use of selective media such as CHROMagar Candida, designed to aid the identification and differentiation of yeasts by the presence of chomogenic substrates in the media that react with species-specific enzymes, producing different coloured colonies [7]. However, limitations of the CHROMagar medium exist, for example it struggles to detect mixed yeast cultures if one species is significantly more prevalent than others showing only the colour of the dominant phenotype [7]. Consequently, it is recommended that the medium is combined with other culture media for isolation of species [7], and remains a presumptive test requiring further mycological analysis for definitive species identification [7]. To improve the diagnostic accuracy of plate cultures, we used the standard medical mycology growth medium SDA to produce mixed cultures of Trichosporon, Candida and Cryptococcus, from which simple antigen solutions could be prepared for testing in an ELISA with MAbs CA7 and TH1. The ELISA results show that both MAbs correctly recognised the presence of T. asahii when grown individually or in combination with C. albicans and C. neoformans, thereby dramatically improving the diagnostic accuracy of the in vitro culture procedure.
It is not known at present whether the MAbs can be used to detect T. asahii and T. asteroides infections without the need for culture of the organisms in vitro. If the diagnostic antigens are present as circulating markers in the bloodstream during disseminated infections or, in the case of SHP, bronchoalveolar lavage fluids (BALf), then the potential exists for the development of point-of-care tests such as lateral-flow devices (LFDs) that can be used to rapidly detect fungal infections without the need for sophisticated laboratory facilities [23,24]. Use of BALf has been demonstrated in the diagnosis of SHP, through the detection of fungal DNA [8], and a similar method might also be used for the immunodetection of T. asahii diagnostic antigens in SHP patients. We have already demonstrated here that the antibodies can be used in a ELISA procedure to improve the diagnostic accuracy of a simple culture procedure employing a standardised growing medium, but the immunoassay test time could be reduced further, from hours for the ELISA to minutes for an LFD [23,24].
It is important to note that while the MAbs developed here detect the two most common causes of invasive trichosporonosis (T. asahii and T. asteroides) and the most common agent of SHP (T. asahii), the other species implicated in superficial and invasive infections (a continually expanding list including T. inkin and T. cutaneum [2,3]) and SHP (currently the only other member of the genus noted as a causative agent is T. mucoides [3,5]) would require differentiation with PCR sequencing of DNA sequences that have sufficient species differences such as the IGS1 region [21]. Nevertheless, the culture-ELISA technique described here represents a significant improvement in the accurate detection and differentiation of Trichosporon from other clinically important yeast pathogens such as Candida and Cryptococcus.

Author Contributions
Conceived and designed the experiments: CRT. Performed the experiments: GD. Analyzed the data: GD. Contributed reagents/materials/ analysis tools: CRT. Wrote the paper: GD CRT.