Deficient Beta-Mannosylation of Candida albicans Phospholipomannan Affects the Proinflammatory Response in Macrophages

Candida albicans produces a complex glycosphingolipid called phospholipomannan (PLM), which is present on the cell-wall surface of yeast and shed upon contact with host cells. The glycan moiety of PLM is composed of β-mannosides with degrees of polymerization up to 19 in C. albicans serotype A. PLM from serotype B strains displays a twofold decrease in the length of the glycan chains. In this study we compared the proinflammatory activities of PLMs purified from C. albicans serotype A and serotype B strains and from a bmt6Δ mutant of C. albicans, whose PLM is composed of short truncated oligomannosidic chain. We found that PLMs activate caspase-1 in murine macrophage cell line J774 independent of the glycan chain length although IL-1β secretion is more intense with long glycan chain. None of the tested PLMs stimulate ROS production, indicating that caspase-1 activation may occur through a ROS-independent pathway. On the other hand, only long-chain oligomannosides present on PLM from serotype A strain (PLM-A) are able to induce TNF-α production in macrophages, a property that is not affect by blocking endocytosis through latrunculin A treatment. Finally, we demonstrate that soluble and not cell surface-bound galectin-3, is able to potentiate PLM-A-induced TNF-α production in macrophages. PLMs from C. albicans serotype B and from bmt6∆ mutant are not able to induce TNF-α production and galectin-3 pretreatment does not interfere with this result. In conclusion, we show here that PLMs are able to evoke a proinflammatory state in macrophage, which is in part dependent on their glycosylation status. Long-glycan chains favor interaction with soluble galectin-3 and help amplify inflammatory response.


Introduction
Candida albicans produces a complex glycosphingolipid (GSL) called phospholipomannan (PLM) [1], which is present at the surface of the cell wall and is shed in contact with host cells [2][3][4][5]. Although the impact of PLM on C. albicans biology is unknown, it is considered to be a virulence factor of C. albicans. PLM induces tumor necrosis factor alpha (TNF-α) secretion from cells of macrophage lineage via a TLR-2dependent pathway [6,7] and macrophage apoptosis via modulation of the ERK pathway [8,9] induced at the time of phagocytosis. The glycan moiety of PLM is unique. It is mainly composed of β-1,2 mannosides with degrees of polymerization up to 19 in C. albicans serotype A strains. PLM from serotype B strains displays shorter β-1,2 mannosides chains with a predominant mannotriose [10]. β-1,2 mannosides by themselves act as stimuli for macrophage activity and are recognized by macrophages through an interaction involving galectin-3, a glycan-binding protein highly produced and secreted by macrophages [11]. Therefore, due to the prominent cell surface expression of β-1,2 mannosides in C. albicans, galectin-3 is able to discriminate between this pathogen [12] and the non-pathogenic yeast Saccharomyces cerevisiae [13,14].
It has been shown that the complete absence of PLM affects the virulence of C. albicans [15]. Alteration of the MIT1 gene in C. albicans resulted in complete absence of glycosylated PLM. Absence of MIT1 leads to no biosynthesis, then no secretion of PLM, and abolishes the immune activities of yeasts both in vitro, toward macrophages, and in animal models [15]. The absence of PLM caused by deletion of MIT1 led to a decrease in virulence of yeasts during both the acute and chronic phases of systemic infection in mice (75 and 50% reduction in mortality, respectively). In vitro, mit1Δ mutant was not able to escape macrophage lysis through downregulation of the ERK1/2 phosphorylation, a previously shown PLM-induced escape mechanism [15].
Recently, a new family of genes encoding βmannosyltransferases (Bmt1-9) has been identified as responsible for adding β-1,2 mannosides to different carriers present in the C. albicans cell wall [16]. Among these nine βmannosyltransferases, Bmt6 was shown to be one of the enzymes specifically involved in β-1,2 mannosylation of PLM. Deletion of BMT6 results in an impairment in the addition of the third β-mannose to PLM backbone [10,17] but has no impact on upstream sphingolipid (IPCs, MIPCs and M (IP) 2 Cs) biosynthesis, meaning that both wild-type (WT) and bmt6∆ mutant yeasts share a common lipid backbone. In addition, it does not affect the addition of β-mannose to other carriers in C. albicans such as phosphopeptidomannan and mannoproteins.
The aim of this study was to investigate the effects of differential β-1,2 mannosylation of PLM on its ability to induce proinflammatory response in macrophages. Therefore, we compared the proinflammatory properties of three different types of PLMs: PLM-A, isolated from SC5314, a WT C. albicans serotype A strain; PLM-B, isolated from the NIHB strain, a serotype B strain and PLM-BMT6∆, isolated from the bmt6∆ mutant. We conclude that PLMs displaying short and truncated glycan chains are still able to lead to inflammasome activation in macrophage, indicating that PLM lipid backbone plays a role in this process. However, the presence of long chains of β-1,2-linked mannosides is essential for the induction of TNF-α production in macrophages and the binding of PLM-A to soluble galectin-3, which may amplify PLM-A-induced inflammatory response.
To avoid possible LPS contamination, which could interfere with the observations, purified PLMs and r-Gal3 were tested for endotoxin contamination (Limulus Amebocyte Lysate -Pyrogent Plus, Lonza -Walkersville, MA). Endotoxins were below the detection limit of the assay (<0.03 EU/ml).

Strains and Growth Conditions
C. albicans wild type strains SC5314 (serotype A, [18]) and NIH B 792 (serotype B, [19]) were used in this study. The mutant strain, bmt6Δ, expressing a truncated PLM was obtained after deletion of BMT6 from strain BWP17 by PCR based gene targeting [20].
Yeast cells were maintained at 4°C on YPD (1% yeast extract, 2% peptone, 2% dextrose)-agar medium. Before each experiment, yeast cells were transferred onto fresh YPD and incubated overnight at 37°C. Yeasts were recovered by centrifugation, resuspended in phosphate buffered saline (PBS) and used at different concentrations.

Cell lines
The mouse macrophage-like J774 cell line (ECAC 85011428), derived from a tumor of a female BALB/c mouse, was from the European Collection of Cell Cultures (ECAC) and is provided by Sigma Aldrich. Adherent cells were cultured at 37°C in an atmosphere containing 5% CO2 in DMEM supplemented with 10% heat-inactivated fetal calf serum (FCS) (Valbiotech, Paris, France), 5 mM L-glutamine, 100 µg/ml streptomycin, and 50 µg/ml penicillin (culture medium). Before use, the cells were gently scraped off with a rubber policeman and, depending on the experiment, either plated onto 8-well Labtek tissue culture chambers (Nunc, Naperville, IL) or onto 24-or 48-well tissue culture dishes.

Stimulation of cells with PLMs
Macrophages (5x10 5 cells/well) were incubated with different concentrations of PLMs for 3 or 5h at 37°C for TNF-α production. For IL-1β production, platted cells were primed for 5h with 500 ng/ml of LPS and then incubated with PLMs for 12 or 24h. After incubation, the supernatants were collected and stored at -80°C until cytokine assays were performed. Cytokine concentrations in cell-free supernatants were measured using a commercial ELISA kit according to the manufacturer's instructions (eBioscience, San Diego, CA). In some experiments, plated cells were incubated for 30 min with 2 ng/ml Latrunculin A (Cayman Chemical, Ann Arbor, MI) prior addition of PLMs in order to inhibit activation depending on internalization.

Binding of rGal-3 to cells
Macrophages (5x10 5 cells/well) platted onto 8-well Labtek tissue culture chambers were incubated with rGal-3 at 10 µg/ml for 1 h. Binding of rGal-3 was stained with the anti-Gal3 antibody (Clone M3/38) and FITC anti-mouse IgG (Southern Biotech). After fixation with 4% paraformaldehyde in PBS, the slides were mounted and the cells were examined by fluorescent microscopy.

Effect of rGal-3 on stimulation
Macrophages (5x10 5 cells/well) plated onto 48-well tissue culture dishes were incubated with rGal-3 at a concentration of 10 µg/ml for 1 h. Cells were then stimulated for 3 to 5 h with or without different concentrations of PLM or with Pam3CSK4 (500 ng/ml) or curdlan (100 and 10 µg/ml) used as control. In parallel experiments, PLM or control stimuli were incubated for 1 h at 37°C with rGal-3 (10 µg/ml) before addition to the cells.
PLM and control stimuli were incubated with or without 10 µg/ml of rGal-3 for 1 h at 37°C. The mixture was then added to plated cells as described above for stimulation. In parallel, PLM and control stimuli were incubated for 1 h with rGal-3 (10 µg/ml) before addition to the cells. rGal-3 activity was inhibited by incubation with 50 mM lactose, a specific inhibitor of Galectin-3, for 15 min before the experiments.

Western blot analysis of activated caspase-1
Total cell proteins were extracted with RIPA buffer (1% NP40, 0.5% Sodium Deoxycholate, 0.1% SDS) for 30 min on ice with occasional checking. Lysates were clarified by centrifugation for 10 min at 12 000 x g at 4°C and then conserved at -20°C. A fixed amount of lysate, determined by the protein concentration, was mixed with 4 x concentrated electrophoresis sample buffer (1 x : 125 mM Tris-HCl, pH 6.8, 2% SDS, 5% glycerol and bromophenol blue) and boiled for 5 min at 100°C. The extracted proteins were then separated by 15 % SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane (Schleicher and Schuell, Dassel, Germany) for 1 h at 200 mA in a semi-dry transfer system. After staining with 0.1% Ponceau S in 5% acetic acid to confirm equivalence of loading and transfer, membrane were saturated with TNT (Tris 10 mM, NaCl 100 mM, Tween 0.1%) containing 5% non-fat dry milk for 1 h at 20°C. The membrane was then probed with anti-caspase-1 p10 (M-20) (diluted 1:1000) in TNT-0.5% non-fat dry milk overnight at 4°C. After several washes, the membrane was incubated for 2 h at 20°C with a 1:1000 dilution of HRPO-conjugated anti-rabbit IgG in TNT-0.5% non-fat dry milk. Finally, proteins were visualized with ECL detection reagents (ThermoFisher, Whaltman, MA) and exposed to hyperfilm ECL.

Fluorescent examination of activated caspase-1
Macrophages (3x10 5 cells/well) were platted overnight onto 8-wells Labtech chambers (Nunc). Cells were then stimulated with LPS for 2h. Stimuli were then added and incubation continued for 4 h. After washing, the cells were stained with the fluorescent specific inhibitor of caspase-1, FAM-YVAD-FMK (5carboxyfluorescein-Tyr-Val-Ala-Asp-fluoromethylketone, FLICA) according to the manufacturer's instructions (Immunochemistry Technologies, Bloomington, MN). The slides were then washed and mounted for microscopic examination.

Statistical analysis
All experiments were repeated at least three times. For cytokine production, the values reported are the means ± SD of the results obtained from three independent experiments. Statistical significance was accepted at P<0.05, determined by ANOVA or student's t test depending on the experiments.

PLMs activate inflammasome in a glycan-independent manner
In order to investigate the effects of PLM glycosylation on macrophage activation, we first purified PLMs from C. albicans serotype A (SC5314), C. albicans serotype B (NIHB) and from the bmt6∆ mutant of C. albicans. Molecular structural differences between the three different types of PLMs are summarized in the Figure 1A. Fluorophore-assisted carbohydrate electrophoresis (FACE) analyses revealed the major oligomannosides released by acid hydrolysis of PLMs ( Figure 1B). As can be seen in the Figure 1 (A and B), PLM from serotype A strains (PLM-A) display a twofold increase in glycan chain length in comparison with PLM from serotype B (PLM-B), and PLM from bmt6∆ mutant (PLM-BMT6∆) which displays a short, truncated glycan chain.
Because inflammasome plays an essential role in antifungal immunity, we tested whether PLMs with different levels of glycosylation were able to induce caspase-1 activation in macrophage. Then, cells were primed with LPS and stimulated with PLMs, ATP or Alum (used as a positive controls for caspase-1 activation) and the cleavage of pro-caspase-1 into active caspase was examined by FLICA (Fluorescent Labeled Inhibitor of Caspases) assay. As shown in the Figure 2A, PLMs (either PLM-A or PLM-BMT6∆) were able to activate caspase-1. To further confirm this result, LPS-primed macrophages were stimulated with PLMs and the activation of caspase-1 was monitored by Western blot. The active caspase-1 (p10) was detected in cell lysates of PLMsstimulated macrophage, irrespective of their glycosylation status ( Figure 2B). As expected, LPS-primed murine macrophages did not exhibit significant caspase-1 activation without secondary stimulation ( Figure 2B).
To further examine the effect of PLMs on inflammasome activation, cells were primed with LPS and IL-1β production was evaluated in cell-free supernatants. As shown in the Figure  2C, PLM-A was able to induce the secretion of IL-1β by primed cells. No cytokine was produce by unprimed cells (data not shown). PLM-B and PLM-BMT6∆ also induced the secretion of IL-1β by primed cells, although the amount of cytokine was lower compared to that obtained with PLM-A.
In order to determine whether PLMs must be internalized to induce the activation of caspase-1, LPS-primed macrophages were treated with a known inhibitor of endocytic activity, Latrunculin A. Interestingly, Latrunculin A was not able to inhibit caspase-1 activation induced by any of the tested PLMs ( Figure 2D). Altogether, these results demonstrate that PLMs induce inflammasome activation and IL-1β production in LPSprimed macrophages.

PLMs Are Not Able to Induce ROS Production in Macrophage
Since reactive oxygen species (ROS) have been shown to be one of the main mechanisms involved in caspase-1 activation [23], the effect of purified PLMs on ROS stimulation was also investigated. First, we checked whether C. albicans yeast cells of serotype A and B display any difference in their capacity to induce ROS generation in macrophage. As shown in the Figure 2E, although both C. albicans serotype A and B were able to stimulate the production of ROS by macrophages, C. albicans serotype A induced higher levels of ROS even in comparison with TPA, used as a positive control. In both cases, ROS generation was inhibited by pretreatment with Latrunculin A, indicating that phagocytosis of the yeasts was required for the induction of ROS production by macrophages. In contrast to C. albicans yeast cells, all tested PLMs did not induce any ROS stimulation ( Figure 2F). This result suggests that PLMsinduced caspase-1 activation is not linked to ROS generation.

Long-chain oligomannosides of PLM are required for induction of TNF-α in macrophage
We have previously shown that PLM is able to stimulate TNF-α production by macrophage in a Toll-like receptordependent manner [13]. Because TNF-α production occurs via a distinct pathway independent of inflammasome and caspase-1 activation, we also checked whether or not glycosylation changes in PLMs would affect their ability to induce TNF-α production. The PLMs isolated from the different sources were first examined for their ability to stimulate TNF-α production by macrophages. As expected [6,13], PLM-A isolated from strain SC5314 (which displays long-chain oligomannosides) was able to induce TNF-α production in a dose-dependent manner and reached a plateau at a PLM-A concentration of 25µg/ml ( Figure 3A). In contrast, macrophages incubated with PLM-B purified from C. albicans serotype B (which displays oligomannoside-type chains of intermediate size) or with PLM-BMT6∆ from yeast mutant lacking BMT6 gene (which displays a short truncated glycan chain) did not stimulate significant TNF-α production even at the highest dose tested ( Figure 3A). In addition, PLM-A induced levels of TNF-α similar to those observed with 100µg/ml of curdlan or 500 ng/ml of Pam3CSK4, used as a positive control for dectin-1 and TLR2 activation, respectively ( Figure 3B). These results suggest that the glycan chain in PLMs plays an essential role in inducing TNF-α production in macrophages.

PLM-induced secretion of TNF-α is independent of internalization
Taking into consideration that the molecular differences between the three different types of PLMs ( Figure 1A) may affect their intermolecular interactions and lead to a different pattern of internalization and/or interaction with cell surface receptors, we next tested whether Latrunculin A would affect PLM-induced TNF-α. As shown in the Figure 3C, PLM-A was the only molecule able to induce TNF-α production and the pretreatment of macrophages with Latrunculin A had no effect on its capacity to induce TNF-α. As expected, PLM-B and PLM-BMT6∆ did not stimulate TNF-α production and this result was not changed by pretreatment of the macrophages with Latrunculin A (Figure 3C). Similar to PLM-A, neither curdlan nor Pam3CSK4-stimulated macrophages were affected by pretreatment with Latrunculin A, demonstrating that internalization had no effect on the response to these soluble stimuli ( Figure 3D). On the other hand, macrophage pretreatment with Latrunculin A led to a significant decrease in TNF-α production induced by C. albicans yeast cells (serotype A), showing that phagocytosis is an important step for host defense against C. albicans ( Figure 3D). C. albicans yeast cells (serotype B), which are similarly phagocytized by macrophages (data not shown), were unable to induce significant production of TNF-α, regardless of whether the macrophages were pretreated with Latrunculin A ( Figure 3D). Taking together, these results demonstrate that PLM-A-induced TNF-α production is not dependent on internalization and may involve direct interaction of PLM-A with cell surface receptors.

Galectin-3 interacts with long-chain oligomannosides of PLM and potentiates its activities on macrophage
We have previously shown that galectin-3 acts as a receptor for β-1,2 linked oligomannosides [11], which in association with TLR2 allows macrophages to discriminate between pathogenic and non-pathogenic yeasts based on their cell surface glycans [13]. Therefore, we investigated whether changes in PLM glycosylation would affect its interaction with galectin-3 and interfere with the PLMs capability to stimulate macrophages. First we verified the galectin-3 expression and galectin-3binding sites levels on the macrophage surface. As expected, J774 macrophages expressed low levels of endogenous galectin-3 ( Figure 4A) and the addition of exogenous rGal-3 led to an enhanced level of galectin-3 at the cell surface ( Figure  4B). Then, we checked whether the enhanced levels of galectin-3 at the cell surface would interfere with PLM-Ainduced TNF-α production in macrophages. As shown in the Figure 4C, there was no significant difference in TNF-α production when rGal-3-pretreated macrophages were stimulated with PLM-A in comparison with PLM-A stimulation alone. PLM-BMT6∆ was not able to induce TNF-α even in rGal-3-pretreated macrophages, which displayed increased levels of TNF-α ( Figure 4C). Thus, PLM-A had no additive or enhanced effect on rGal-3-pretreated macrophages. Similar results were obtained with curdlan or Pam3CSK4, both of which induced similar levels of TNF-α in rGal-3-pretreated or not macrophages ( Figure 4D).
Different results were obtained when rGal-3 was preincubated with increasing concentrations of PLMs prior to addition to macrophages ( Figure 5). In this case, compared to macrophages stimulated with rGal-3 or PLM-A alone, the complex rGal-3/PLM-A induced a significant increase in TNF-α production by macrophages ( Figure 5A). However, different results were obtained with PLM-B and PLM-BMT6∆, both of which did not stimulate TNF-α production even after preincubation with rGal-3 ( Figure 5B and 5C, respectively). The addition of lactose, a specific inhibitor of galectin-3, during the preincubation step with rGal-3, decreased but did not abolish the enhancing effect of rGal-3 on PLM-A activity (data not shown). The enhancing effect of rGal-3 on PLM-A activity appears to be specific since no significant effect was observed when curdlan or Pam3CSK4 were preincubated with rGal-3 ( Figure 5D). These results demonstrate that soluble galectin-3 may interact with long-chain oligomannosides of PLM-A and potentiates its effects on macrophage activation.

Discussion
The present study was undertaken to examine the role of the glycan moiety of PLM, a glycolipid present in the cell-wall and that plays an important role in the interplay between C. albicans and host immune cells [4,6,7,9]. Through a series of experiments, we demonstrated that differential glycosylation of PLM-A, PLM-B and PLM-BMT6∆ does not affect their capacity to induce caspase-1 activation in murine macrophage-like cell line J774. Although each PLM induced the secretion of IL-1β, a higher production was observed after stimulation with PLM-A. Long chains of β-1,2-linked mannosides on PLM are essential for PLM-induced TNF-α since PLMs with short (PLM-B) or truncated (PLM-BMT6∆) β-1,2-linked mannosides were not able to stimulate TNF-α production in macrophages. In addition, long glycan chains on PLM-A favors the formation of complexes with exogenous galectin-3, a glycan-binding protein secreted by macrophages [24] and that binds to β-1,2 mannosylated compounds [11].
We have previously shown that PLM, a C. albicans cell-wall glycolipid bearing β-1,2-linked oligomannosides, induces TNFα production through an interaction involving galectin-3 and TLR2 [2,11,25]. PLM has been shown to interact with host cells and to induce both pro-inflammatory cytokine production [4,6] and apoptosis through the regulation of ERK signaling pathway via regulation of phosphatase MKP-1 [9]. However, until now, the role of the glycan moiety of PLM in macrophage activation has not been completely investigated since there were no mutant yeasts lacking specific mannosyltransferases required for PLM glycosylation. We recently obtained a series of mutants of C. albicans altered in their capability to mannosylate different cell wall components [16]. Among them, bmt6∆ mutant was shown to be altered in its ability to elongate the glycan arm of the PLM molecule [17], resulting in a short oligommanosidic moiety similar to one of the major PLM components present in C. albicans serotype B [10].
Recently, it was demonstrated that C. albicans triggers activation of the inflammasome [26,27] and, therefore, we hypothesized that PLMs may act as a stimulus that activates the inflammasome in macrophages. Surprisingly, both mannosylated PLM and PLM bearing short chains of oligomannosides were able to induce caspase-1 cleavage, a marker of inflammasome activation. However, PLMs-induced caspase-1 activation is not directly related to the glycosylation status of PLM indicating that the lipid core of PLM, which is similar for all PLMs studied, accounts for inflammasome activation. Accordingly, soluble galectin-3 was not able to potentiate PLMs-induced caspase-1 activation (data not shown).
Even though PLMs induce the activation of caspase-1 and secondarily the secretion of IL-1β, our results demonstrated that none of the PLMs tested were able to induce ROS production, which suggests that PLM-induced inflammasome activation is mediated by Syk-ROS independent pathway, as opposed to other PAMPs such as beta-glucans [26,28]. Since the lipid moiety is involved in the pro-apoptotic activity of PLM through modulation of MKP-1, a phosphatase related to ERK [29], it is possible that the lipid core of PLM plays a major role in caspase-1 activation, independently of ROS production or phagocytosis. Indeed, the structure of the lipid core of PLMs resembles that of lipids present in ceramides [1] which are known to activate Nlrp3 inflammasome [30]. Experiments are in progress to define the role of the lipid core of PLM in inflammasome activation and the IL-1β secretion processes.
PLM isolated from C. albicans serotype A, whose mannosylated arm is composed of long chains of oligommannosides with up to 19 β-1,2-linked mannoses [1], was able to induce strong TNF-α production in macrophages. In comparison, PLM from C. albicans serotype B whose glycan moiety is composed of up to 10 mannoses with predominant trimannosides [10], and PLM from bmt6∆ mutant of C. albicans, which presents only short chains of oligomannosides with up to three linked mannoses, did not stimulate the cells to produce TNF-α. This is in agreement with our previous results [25], which showed that the activity for β-1,2-linked oligomannosides in macrophages was achieved with chains presenting at least four mannose residues, with optimal activity being observed with chains composed of at least eight β-1,2-linked mannoses. This emphasizes the predominant role of sugars in PLM activity since neither PLM-B nor PLM-BMT6∆ were able to stimulate the cells. We also demonstrated that phagocytosis was not necessary for the PLM-A-induced proinflammatory activity and, therefore, the inhibition of actin polymerization by Latrunculin-A did not modify its properties. This result suggested that long chains of β-1,2-linked mannoses present on PLM-A stimulate TNF-α production by interacting directly with its receptor at the cell membrane. Therefore, enhanced complex formation and endocytosis of PLM displaying incomplete glycosylation do not account for the decreased capability of PLM-B or PLM-BMT6∆ to stimulate macrophages.
We have previously shown that galectin-3 binds to β-1,2 mannosides from C. albicans [11] and is required to discriminate between the pathogenic yeast C. albicans from the non-pathogenic Saccharomyces cerevisiae [13]. In this study, we demonstrated that soluble rGal-3 potentiates the PLMinduced stimulation of macrophages, as demonstrated for PLM-bearing long chain of β-1,2-linked mannoses (PLM-A). It is not known whether the formation of complexes between PLM and galectin-3 would enhance secondary binding to TLR2 or to other receptors known to interact with galectin-3 and favor recognition of PLM.
In contrast to soluble galectin-3, increasing the surfacebound galectin-3 level did not result in any further increase in TNF-α production by macrophages. Galectin-3 has been shown to associate with TLR2 [13] but also with other membrane receptors such as Dectin-1, thus favoring the interaction of the yeasts with the macrophage plasma membrane [31]. Galectin-3 is able to interact with other glycoproteins present at the cell membrane, which could increase the binding of PLM to the plasma membrane The mixtures were then added to J774 macrophages and incubated for an additional 4h. TNF-α concentration in cell-free supernatants was determined by ELISA. (D) Exogenous rGal-3 (10 µg/ml) was preincubated (■) or not (□) with 10 µg/ml of curdlan or 500 ng/ml of Pam3CSK4. Results are represented as the mean ± standard deviation from 3 independent experiments. P values refer to the statistical differences between the effect exerted by galectin-3 alone and in association with PLMs. *p<0.05; **p<0.01. doi: 10.1371/journal.pone.0084771.g005 receptors. In our experimental settings, we can not rule out that surface-bound galectin-3 may indeed lead to an increase in PLM-binding to the cell surface. However, since no increased stimulation was observed in comparison with PLM-A pretreated with rGal-3, we suggest that soluble galectin-3 may affect PLM complex formation, which leads to enhanced activation. Indeed, it has been recently shown that LPS preincubation with galectin-3 decreases the LPS concentration threshold for neutrophil activation [22].
The results of this study show that PLM is able to activate inflammasome pathway through a ROS-independent mechanism. This activity seems to be related to the lipid moiety of the molecule since all tested PLMs were able to induce caspase-1 activation and IL-1β secretion. On the other hand, the induction of TNF-α production is dependent on the glycan moiety displayed by PLM; PLMs-bearing short length or truncated β-1,2-linked mannosides were not able to stimulate TNF-α production in macrophages. PLM activity is not influenced by phagocytosis, suggesting that the interaction with receptors occurs at the cell membrane. Galectin-3, a host cell receptor for β-1,2-linked mannoses, enhances PLM-induced TNF-α production and may act as an amplifier of inflammatory response caused by C. albicans. Altogether, our results show that depending on the pathway examined, both the lipid core and the glycan moiety of PLM are important for macrophage activation.