Role of LytF and AtlS in eDNA Release by Streptococcus gordonii

Extracellular DNA (eDNA) is an important component of the biofilm matrix produced by many bacteria. In general, the release of eDNA is associated with the activity of muralytic enzymes leading to obvious cell lysis. In the Gram-positive oral commensal Streptococcus gordonii, eDNA release is dependent on pyruvate oxidase generated hydrogen peroxide (H2O2). Addition of H2O2 to cells grown under conditions non-permissive for H2O2 production causes eDNA release. Furthermore, eDNA release is maximal under aerobic growth conditions known to induce pyruvate oxidase gene expression and H2O2 production. Obvious cell lysis, however, does not occur. Two enzymes have been recently associated with eDNA release in S. gordonii. The autolysin AtlS and the competence regulated murein hydrolase LytF. In the present report, we investigated the role of both proteins in the H2O2 dependent eDNA release process. Single and double mutants in the respective genes for LytF and AtlS released less eDNA under normal growth conditions, but the AtlS mutant was still inducible for eDNA release by external H2O2. Moreover, we showed that the AtlS mutation interfered with the ability of S. gordonii to produce eDNA release inducing amounts of H2O2. Our data support a role of LytF in the H2O2 eDNA dependent release of S. gordonii as part of the competence stress pathway responding to oxidative stress.


Introduction
The biofilm developmental process requires the release of extracellular polymeric substances (EPS) by the biofilm forming community [1,2]. The EPS is commonly composed of protein, polysaccharides, lipids and extracellular DNA (eDNA) [3,4]. The presence of eDNA during the developmental process is important [5], since treatment of developing or preformed biofilms with DNA degrading enzymes disrupts the biofilm structure and stability [6,7,8]. In general, eDNA found in the biofilm EPS seems to be of microbial chromosomal origin [9,10,11]. Investigations on the integrity of the eDNA revealed largely intact DNA still carrying genomic information, which is further supported by the observation that eDNA is also a source for horizontal gene transfer [12,13]. A recent detailed oligonucleotide array based study using a non-domesticated Bacillus subtilis strain showed that the biofilm recovered eDNA includes the whole genome without specific gene preferences [14]. Earlier studies with other bacterial species showed that randomly selected genes on different chromosomal locations are present in eDNA [10] suggesting that chromosomal DNA is released to serve as eDNA during biofilm development.
Different release/production mechanisms for eDNA of chromosomal origin seem to exist. For example, the bacteriolytic dependency of eDNA release was demonstrated for several species and is ultimately linked to bacterial cell death [11,15,16]. The regulatory relationship between eDNA release and cell death has been studied in detail in Gram-positive Staphylococcus aureus [17,18,19]. The Staphylococcal Cid/Lrg system encodes proteins analogous to the bacteriophage-encoded holins and antiholins. Initial studies suggest that the LrgA and CidA proteins function in similar mechanisms as the holins/antiholins in S. aureus, ultimately activating murein hydrolases leading to bacterial cell lysis [17,18,19]. Homologs of Cid/Lrg can be found in several species, including cariogenic Streptococcus mutans. Interestingly, the Cid/Lrg system in S. mutans is involved in several other cellular processes including competence development and oxidative stress tolerance, suggesting a connection between general stress and lysis dependent eDNA release in oral streptococci [20,21]. In the opportunistic pathogen Pseudomonas aeruginosa, phage inductions in biofilms are implicated in the release of DNA as a result of phage mediated cell lysis [11,22]. Other examples of lysis dependent eDNA release are the autolysin AtlE dependent eDNA release in Staphylococcus epidermidis [23] and the gelatinase GelE and serine protease SprE dependent eDNA release in Enterococcus faecalis [16].
An alternative to the cell-lysis dependent eDNA release mechanisms has been suggested in two recent studies in E. faecalis [24] and B. subtilis [14] where a lysis-independent eDNA release mechanism has been proposed. In E. faecalis well-defined structures of eDNA were observed supporting early biofilm development. However, no intracellular components indicative of cell lysis could be detected in cell free supernatants during the early biofilm developmental stage. Furthermore, cells implicated in the release of eDNA had an active membrane potential excluding a connection with bacterial cell death [24]. B. subtilis on the other hand has a mechanism to release eDNA in the late exponential phase. The authors of this study confirmed lysis-independence genetically by constructing several mutant strains with genes involved in bacterial lysis showing they are not reduced in the eDNA release. The release process appeared to be regulated by the B. subtilis early competence genes [14].
Streptococcus gordonii belongs to the group of early oral biofilm formers and it's presence is critical for subsequent biofilm development since it provides attachment sites for other species [25]. S. gordonii as well as several other oral streptococci are known for their ability to produce competitive amounts of hydrogen peroxide (H 2 O 2 ) during aerobic growth [26]. H 2 O 2 production inhibits growth of competing species, but also induces the release of eDNA [10,27,28]. Furthermore, H 2 O 2 has been demonstrated as the sole agent responsible for triggering the release process. The addition of H 2 O 2 to S. gordonii grown under non-H 2 O 2 -producing conditions during static growth induces eDNA release, but no detectable autolysis [12]. Involvement of bacteriolytic enzymes in the eDNA release of S. gordonii, however, has been shown by two recent studies [13,29]. Deletion of the autolysin AtlS causes a major decrease in eDNA release [29]. In addition, inactivation of the competence dependent murein hydrolase LytF reduced the gene transfer in a co-culture of competent S. gordonii with the LytF mutant about 100 fold. However, direct eDNA concentrations were not determined [13].
The present report presents a further characterization of the role of LytF and AtlS in the H 2 O 2 dependent eDNA release of S. gordonii.

Bacterial Species and Culture Conditions
All S. gordonii strains used in this study listed in Tab. 1 were routinely grown aerobically (5% CO 2 ) at 37uC in BHI (Brain Heart Infusion; Difco, Sparks, MD) unless otherwise stated. For antibiotic selection, cultures were supplemented with the following antibiotics: erythromycin at 5 mg ml 21 and kanamycin at 300 mg ml 21 .

Growth Kinetics
The growth of static wild type and mutant strains was monitored using a Bioscreen C analyzer version 2.4 (Oy Growth

DNA Manipulations
Standard recombinant DNA manipulations were used [30]. PCR was performed with a G-Storm GS1 thermocycler (GeneTechnologies; Essex, UK) according to the manufacturer's protocol. Phusion H DNA polymerase was obtained from New England Biolab. Oligonucleotides (Tab. 1) were designed using sequence data obtained from the Los Alamos National Laboratory Oral Pathogens Sequence Database (http://www.oralgen.lanl.gov) and synthesized by Integrated DNA Technologies (Coralville, IA).

Construction of Mutant Strains
The lytF mutant strain was constructed by transforming chromosomal DNA from strain SGH24 [13] carrying a lytF deletion using a transformation protocol reported earlier [31]. To construct a double AtlS/LytF K.O. mutant, the AtlS mutant was chosen to replace the kan antibiotic cassette with an ermAM cassette for compatibility with the lytF K.O. Replacement of the antibiotic cassette was done via double-crossover homologous recombination using a overlap PCR strategy. To generate the overlap PCR constructs, two fragments corresponding to around 500 bp of the upstream and downstream sequences of atlS were amplified by PCR, using Phusion H DNA polymerase with the oligonucleotides AtlS up-F/AtlS up-R and AtlS down-F/AtlS down-R. Each of the oligonucleotides listed in Tab.1 as up-R and down-F incorporated 25 bases complementary to the erythromycin resistance cassette, ermAM [32]. The erythromycin resistance cassette ermAM was amplified by PCR using the primers ermAM F and ermAM R as described before [31]. All three PCR amplicons were purified with the QIAGEN PCR purification kit and mixed in a 1:1:1 ratio. The mixture served as a template for a second round PCR with the appropriate up F and down R primers. The resulting PCR amplicons were transformed into DL1 AtlS to generate the deletion mutant DL1 AtlS-1. To create the double mutant, the LytF mutation was transformed into DL1 AtlS-1 as described above.

Construction of Renilla Bioluminescent Reporter-strain and Renilla Assay
The renilla reporter strain was constructed via a four-piece overlapping PCR ligation strategy similar to the strategy described above. The renilla gene was set under the control of the ldh (lactate dehydrogenase) promoter from S. gordonii. The renilla gene was inserted downstream of the ldh stop codon to leave the ldh gene intact. Briefly, about 1000 bp of the 59 ldh open reading frame including the ribosome binding site (rbs) of the ldh promoter were amplified with oligonucleotides Sg-ldh-up-F/Sg-ldh-up-R-ren, the renilla gene was amplified from plasmid pRL-TK (gift from Dr. Ralf Janknecht, University of Oklahoma Health Sciences Center) with oligonucleotides Sg-ren-F/Sg-ren-R. The Sg-ren-F primer introduced the ldh rbs on the 59 end of the renilla gene. The ermAM gene cassette for selection of PCR product integration into the chromosome was amplified using oligonucleotides Sg-ren-erm-F/ Sg-ren-erm-R and the ldh downstream fragment (about 1000 bp) was amplified with oligonucleotides Sg-ldh-down-F/Sg-ldh-down-R. Oligonucleotides were constructed with overlapping sequences as shown in Tab. 1. All four PCR amplicons were purified with the QIAGEN PCR purification kit and mixed in a 1:1:1:1 ratio. The mixture served as a template for a second round PCR with the appropriate up F and down R primers. The resulting PCR amplicons were transformed into DL1. Successful transformation was confirmed by testing several transformants for renilla reporter gene activity. The LytF and AtlS mutation were generated by transformation of chromosomal DNA from DL1-AtlS and SGH24, respectively. To assay for renilla activity, 100 ml of an exponentially growing culture was mixed with 0.5 ml of ViviRen TM Live Cell Substrate (Promega) from a 3.7 mg/1 ml stock solution. Bioluminescence was determined with a Modulus Luminometer (Turner BioSystems).

RNA Isolation, cDNA Synthesis, and Real-time RT PCR
RNA was isolated using a Qiagen RNeasy kit, and cDNA was synthesized using qScript TM cDNA synthesis kit (Quanta Biosciences) according to the manufacturer's protocol. Real-time RT PCR was performed to determine specific cDNA copies with the comparative threshold cycle (C T ) method using a MyiQ single-color real-time PCR detection system (Bio-Rad) and PerfeCta TM SYBR H Green SuperMix for iQ TM (Quanta Biosciences). Relative changes in cDNA copies representing differential gene expression were calculated using the DC T method described previously (62). The 16S rRNA gene was used as the housekeeping reference gene using the 16S rRNA oligonucleotides described in Tab. 1.

Determination of H 2 O 2 Concentration
The concentration of H 2 O 2 in liquid cultures was determined using a modification of the protocol described by Gilliland (12). Cell-free culture supernatants (40 ml) were mixed with 160 ml of freshly prepared 0.1 M sodium acetate (pH 4.5) containing 0.1 mg horseradish peroxidase (Thermo Scientific) and 10 ml of 1 mg/ml o-dianisidine (Alfa Aesar) in methanol. The reaction mixture was incubated at room temperature for 10 min and protected from light before A 415 nm was determined using a microplate reader (model 680; Bio-Rad). The concentration was calculated from a standard curve prepared in the same medium or buffer using a serial dilution of a commercial 30% H 2 O 2 solution in MilliQ water. The concentration of the initial dilution was determined spectrophotometrically (e 240 = 43.6/M?cm) using a SmartSpec Plus UV-visible spectrophotometer (Bio-Rad) before each new experiment. The detectable range was 0.1 to 4.0 mM H 2 O 2 in BHI.

Observation of eDNA Release
The amount of eDNA in liquid cultures was measured directly by quantitative real-time PCR. A 2-ml aliquot of cell-free culture supernatant was mixed with 8 ml molecular-grade water (G- Biosciences), 12.5 ml PerfeCta TM SYBR H Green SuperMix for iQ TM (Quanta Biosciences), 1.25 ml of primer 16S rRNA Sg-F, and 1.25 ml primer 16S rRNA Sg-R from a 10 mM stock solution. The PCR was performed in a MyiQ single-color real-time PCR detection system (Bio-Rad) and included one cycle of 95uC for 3 min, followed by 40 cycles of 95uC for 15 s and 55uC for 1 min. The DNA concentration was calculated based on average threshold cycle values against a 10-fold dilution series of purified DL1 genomic DNA in the same medium. The detectable range was 0.001 to 100 mg/ml DNA. The concentration of the standard was adjusted using a NanoDrop-1000 spectrophotometer (Thermo Scientific).

Statistical Analysis
Statistical significance was calculated using a two-sided Student's t-test and Quickcalcs online calculator (http://www. graphpad.com/quickcalcs). P values less than 0.05 were considered statistically significant.

Influence of Aeration on Growth of Wild Type, AtlS, LytF and AtlS/LytF Mutants
Previous experimental results linked the production of H 2 O 2 to the release of eDNA in S. gordonii [12]. H 2 O 2 is produced by the pyruvate oxidase (SpxB) under aerobic growth conditions and has a self-inhibitory effect on the producing species [12,28]. To determine whether the introduction of the respective AtlS, LytF and AtlS/LytF mutations into S. gordonii causes any growth defects, growth was monitored under aerobic and static conditions, which has been shown to abolish H 2 O 2 production [12]. During static growth, both the wild type and the LytF mutant showed nearly identical growth patterns (Fig. 1A). The AtlS and AtlS/LytF mutant strains showed a slightly reduced growth rate, but all four  strains reached the same final bacterial density. In contrast, when cells were grown aerobically with maximal H 2 O 2 production, the wild type and the LytF mutant strains reached stationary phase earlier than the AtlS and AtlS/LytF mutants, while the growth rate was identical (Fig. 1B). This observation is reminiscent of results obtained with aerobically grown streptococcal cells in the presence of catalase, which allows for increased cell density by avoiding the growth inhibitory effect of H 2 O 2 [33]. The observed higher cell densities of the AtlS and AtlS/LytF mutants suggest an impaired H 2 O 2 production. To exclude, however, that bacterial aggregation and therefore increased precipitation of bacterial aggregates is a result of the here observed growth phenotype cells were examined microscopically. Aerobically grown cells showed tangled and elongated streptococcal chains for the AtlS and AtlS/ LytF mutants, while the wild type and LytF mutant grew in short chains (Fig. 2). This excludes a possible effect of cell aggregation on the bacterial density measurements. In conclusion, the observed growth phenotypes suggest that either the AtlS mutation confers some kind of resistance to the produced H 2 O 2 or a lower H 2 O 2 production in the AtlS mutants during aerobic growth allowing for higher final cell densities.

Differential H 2 O 2 Production by the Wild Type, AtlS, LytF and AtlS/LytF Mutants
The observed difference in cell density of aerobically grown cultures could be the result of changes in H 2 O 2 production of the respective mutant strains. Supporting a difference in H 2 O 2 production, growth under aerobic conditions also leads to a clear difference in colony size between wild type, AtlS, LytF and AtlS/ LytF mutants (Fig. 3). This was not observed during incubation in an anaerobic growth chamber (data not shown). The production of H 2 O 2 was therefore monitored and no H 2 O 2 production was observed during static growth as reported before [12]. During aerobic growth, both the wild type and the LytF mutant showed peak production of H 2 O 2 during exponential growth, reaching about 1.4 mM when entering the stationary phase (Fig. 4), consistent with earlier reports [12]. In contrast, the AtlS and AtlS/ LytF mutants only produced up to 0.6 mM when stationary phase was reached, about 40% of the wild type production capacity (Fig. 4). The net H 2 O 2 production of the AtlS mutant was determined with cells grown aerobically to mid-exponential phase, showing about a 25% reduction when compared to the wild type (data not shown). These results suggest that the introduction of the AtlS mutation had a general effect on the H 2 O 2 production capacity of strain DL1.

Reduced Production of H 2 O 2 is a Result of Decreased spxB Expression
H 2 O 2 in S. gordonii originates mostly from the enzymatic activity of the pyruvate oxidase, SpxB (gene spxB) [34,35]. SpxB catalyzes the conversion of pyruvate to acetyl phosphate, which subsequently is converted to acetate by acetate kinase [34]. The expression of the spxB gene was determined from cells grown aerobically to mid-exponential phase. In agreement with the observed lower H 2 O 2 production shown in Fig. 4, cells carrying the AtlS mutation showed a statistically significant 2.5 to 4 fold lower spxB expression, respectively (Fig. 5).

Release of eDNA
AtlS and LytF have been implicated in the release of eDNA in S. gordonii [13,29]. However, the eDNA release of the lytF mutant was not determined directly. To learn how the AtlS, LytF and AtlS/ LytF mutation interferes with the ability of S. gordonii to release eDNA, cells were first grown under aerobic conditions to midexponential phase and the eDNA concentration measured in the supernatant. All mutant strains had a significant reduction in eDNA release, ranging from a 26-fold reduction for the AtlS mutant, 10-fold reduction for the LytF mutant and 14-fold reduction for the AtlS/LytF double mutant strain (Fig. 6A).
Since the release of eDNA in S. gordonii can be induced by H 2 O 2 [12], it was important to determine if the eDNA release could still be induced in the mutant strains. Thus, cells were grown under static, non-producing conditions and 2 mM H 2 O 2 was added to the cultures during mid-exponential growth. The cells were further incubated for 5.5 hours and the concentration of eDNA de-termined in the supernatant. As previously reported, the amount of eDNA released is lower when non-producing cells are challenged with H 2 O 2 as compared to aerobically grown cells [12]. The AtlS mutant showed a reduced amount of released eDNA compared to the wild type, albeit not statistically significant (Fig. 6B). In contrast, the LytF and AtlS/Lytf mutant strains showed a statistically significant 5-fold reduction in the detectable eDNA (Fig. 6B), indicating that the LytF mutation interfered with the H 2 O 2 induced eDNA release.

Contribution of AtlS and LytF to S. gordonii Bacteriolysis
To determine the contribution of AtlS and LytF to cell lysis, a renilla luciferase reporter protein strain was constructed [36]. This renilla luciferase (36 kDa) catalyzes the emission of visible light in the presence of oxygen and the substrate coelenterazine, and does not require any other co-factors [37]. In addition, the enzyme is highly stable over hours in supernatants [38]. The  measurement of reporter protein activities in supernatants has been used before to determine bacterial lysis in connection with eDNA release [14,15,39]. The cells were grown as shaken cultures for maximal eDNA release and to avoid any oxygen limitation for the renilla enzyme. No difference in renilla activity was determined when cell suspensions were measured (Fig. 7A). The expression of the renilla reporter-fusion is therefore not influenced by the introduced mutations allowing for direct comparison of all strains. Next, the filter-sterilized supernatant was measured to determine the activity of the released renilla protein and normalized to the renillla activity of the respective cell suspension to determine the percentage of released renilla activity (Fig. 7B). The percentage of extracellular renilla activity was 0.52% 60.2 for the wild type, 0.22% 60.1 for the AtlS mutant and 0.49% 60.2 for the LytF mutant. The difference between wild type and AtlS mutant is statistically significant.

Discussion
The present report aims to better understand the mechanism of eDNA release in the oral commensal S. gordonii. We initially reported the observation of growth phase dependent eDNA release in S. gordonii and Streptococcus sanguinis [10]. The eDNA release was linked to the oxygen dependent metabolic enzyme pyruvate oxidase (SpxB). A deletion of the spxB gene diminished the release of eDNA in both organisms. Further examination revealed that the metabolic by-product of SpxB activity, H 2 O 2, is responsible for the observed eDNA release based on several lines of evidence: i) addition of the H 2 O 2 degrading catalase or peroxidase decreased the released eDNA over 100 fold ([10] and unpublished result), ii) cells grown under anaerobic conditions producing no H 2 O 2 failed to generate eDNA [12,29], and iii) addition of physiological amounts of H 2 O 2 to either anaerobically grown cells or to the spxB mutant triggered the release of eDNA [10,12]. Interestingly, we did not observe an eDNA release associated cell density decline as reported for other species. Incubation for more than 90 hours under maximal H 2 O 2 producing conditions did not result in changes to the final cell density determined spectrophotometrically. An autolysis assay known to induce bacteriolytic activity in other species failed to induce lysis in S. gordonii and S. sanguinis regardless if H 2 O 2 was present or not [10]. Furthermore, no difference of the intracellular nucleoside triphosphate ATP was measured in the supernatant of the wild type vs. the SpxB mutant, further confirming that no general lysis is associated with eDNA release in both oral streptococci [10]. We concluded that both streptococcal species used in our studies did not lyse substantially under conditions known to cause lysis of other firmicutes, for example E. faecalis and S. aureus [10,16,40].
Our data, however, is in contrast to two recent observations implicating bacteriolytic activity to the release of eDNA in S. gordonii [13,29]. First, the autolysin AtlS was identified to be essential for autolysis in S. gordonii. A knock-out mutant did not release eDNA at all [29]. Second, the muralytic LytF protein seemed to be involved in the competence dependent release of eDNA [13]. LytF is a functional analog to the well-characterized Streptococcus pneumoniae murein hydrolase CbpD [41]. Both AtlS and LytF have proven murein-hydrolyzing activity in in vitro zymorgraphic assays [13,29]. LytF however, is only active during competence development and its muralytic activity seems to be limited to a sub-fraction of cells [13]. The here used DL1 wild type and AtlS mutant were identical to the one used in [29]; however, the LytF mutation, originally in another S. gordonii strain, NCTC 7865, was transferred to the DL1 strain. The original AtlS study used 1/4-strength BHI medium supplemented with 10 mM sucrose [29]. Since sucrose causes carbon catabolite dependent repression of spxB expression [42], our study used full strength BHI without any added carbohydrates.
Quantitation of the released eDNA in the wild type, AtlS, LytF and AtlS/LytF mutant strains showed an obvious reduction in the produced eDNA in the mutants, ranging from 10 to 26 fold less eDNA in the supernatant compared to the wild type. This initially supported an involvement of AtlS and LytF in the eDNA release process. Further investigation, however, of the H 2 O 2 production pattern showed that the AtlS mutation affected the concentration of H 2 O 2 during the exponential growth phase leading to a twofold lower end concentration of H 2 O 2 in the supernatant. The reduction was the result of a decreased spxB expression in the mutants carrying the AtlS mutation. This has a profound effect on the eDNA release, since our earlier data showed that a threshold H 2 O 2 concentration is required for eDNA release [12]. Detectable amounts of eDNA were only released when the H 2 O 2 concentration reached amounts higher then 0.6 mM, with the maximal release of eDNA at concentrations around 1 to 2 mM [12]. We suspected that the AtlS mutant did not produce sufficient amounts of H 2 O 2 to trigger the eDNA release process. To confirm this, we added H 2 O 2 to exponentially growing AtlS mutant cells under non eDNA releasing conditions and confirmed that the AtlS mutant was still inducible for eDNA production comparable to wild type amounts. Our new results argue against an involvement of AtlS in the H 2 O 2 dependent eDNA release process, however, the effect of AtlS on H 2 O 2 production and spxB expression requires further investigation to understand the causal relationship between these observed phenotypes.
The eDNA release was not associated with a detectable cell lysis as reported in this study and earlier [10], suggesting that only a subpopulation of cells lyse and releases eDNA or that the actual release process is not caused by complete bacterial lysis, leaving the cell envelope mostly intact. The study by Berg et al. demonstrated that most of the S. gordonii cells are not affected by the muralytic activity of LytF and that only a fraction of the cell population is lysed. To determine the contribution of AtlS and LytF to cell lysis, we measured the release of a reporter protein into the medium during aerobic growth. This growth condition promoted the highest amount of eDNA release [10,12,29]. Interestingly, the activity measured in the supernatant was comparable between the wild type, the AtlS mutant and the LytF mutant and about 1000 fold over growth medium background. After calculating the percentage of renilla activity in the supernatant a two-fold significant reduction of activity for the AtlS mutant was detectable with 0.52% 60.2 for the wild type and 0.22% 60.1 for the AtlS mutant. The LytF mutant was comparable to the wild type with 0.49% 60.2, respectively. For comparison, the CbpD mutant encoding the functional LytF analog of S. pneumoniae had a 80-fold reduction in a b-galactosidase release assay, when compared to the wild type [43]. The S. aureus cidA mutant encoding the murein hydrolase regulator involved in DNA release had a 10-fold difference in the b-galactosidase release assay, when compared to the wild type [15]. The low extracellular activity of the renilla enzyme of the wild type, AtlS and LytF mutant suggests that complete cell lysis is not a major factor in the eDNA release process.
Our data, however, suggests that LytF is the responsible enzyme for the here-observed eDNA release, since the LytF mutant strain is no longer inducible for eDNA release. An involvement of LytF also makes sense considering that its gene is part of the competence system. The S. gordonii competence system is induced under H 2 O 2 producing conditions [12]. Competence in general is considered a major stress response allowing for the uptake of environmental DNA for repair and recombination [44]. S. gordonii appears to release eDNA as part of competence development under stress situations. Further research is required to understand to what extend LytF lyses cells and releases eDNA, but not cellular content such as enzymes and ATP as shown before.