HIV Tat Induces Expression of ICAM-1 in HUVECs: Implications for miR-221/-222 in HIV-Associated Cardiomyopathy

Cardiac involvement is a well-documented complication of human immunodeficiency virus-1 (HIV-1) infection. Previous studies have demonstrated increased adhesion of monocytes to human vascular endothelial cells in HIV-infected individuals. HIV Tat protein, which is the transactivator of transcription (Tat), plays a key role in activating endothelial cells. In the present study, we demonstrated that exposure of HUVECs to HIV Tat protein resulted in induced expression of cell adhesion molecules specifically ICAM-1, leading to increased adhesion of monocytes to the endothelium. This effect of Tat was mediated through activation of mitogen-activated protein kinases and downstream transcription factor NF-κB. Increased expression of ICAM-1 was regulated by microRNA (miRNA) miR-221 and to some extent by miR-222, both of which are known to target ICAM-1. Functional inhibition of the respective miRNAs with anti-miR oligonucleotides resulted in induction of ICAM-1 protein in HUVECs. Furthermore, Tat-stimulated regulation of ICAM-1 via miR-221/-222 involved the NF-kB-dependent pathway. Functional implication and specificity of up-regulated ICAM-1 was confirmed using the ICAM-1 neutralizing antibody in the in vitro cell adhesion assays. These findings were further confirmed in vivo using the HIV transgenic (Tg) rats. These animals not only demonstrated increased expression of ICAM-1 mRNA, with a concomitant reduction in the expression of miR-221 in the aorta and heart, but also had increased expression of the ICAM-1 protein that was predominantly in the endothelial cell layer. Taken together, these findings implicate that Tat-mediated induction of ICAM-1 expression plays a critical role in monocyte adhesion observed in HIV-1-associated cardiomyopathies.


Introduction
Several postmortem studies on AIDS patients have shown clear signs of cardiomyopathies [1]. Clinical studies on HIV-infected patients also provide evidence of progressive cardiac complications following HIV-1 infection [2,3]. While the advent of anti-retroviral therapy has decreased the incidence of HIV-1 cardiomyopathy (HIVCM), its prevalence is actually on a rise. The mechanism(s) by which HIV-1 induces inflammation of the heart are not well understood, but are likely multifactorial in nature. HIV-1 viral protein Tat that is released by infected monocytes and taken up by neighboring cells has been shown to facilitate interaction of the monocyte with the endothelium [4,5], resulting in the recruitment of monocytes into the extravascular tissue. This process, in turn, contributes to destruction of the tissue parenchyma and cellular architecture, a classic feature observed in patients with AIDS [5].
Accumulation of monocytes within the tissue leads to tissue damage and dysfunction. Monocyte adhesion is a dynamic, multistep process involving initial ''rolling'' of cells along the vessel endothelium in response to inflammatory mediators, arrest to endothelium and subsequent strong adhesion to the systemic vasculature [6]. Interaction of endothelial adhesion molecules with their cognate ligands on monocytes is critical for this process.
Up-regulation of adhesion molecules such as ICAM-1 and VCAM-1 is pivotal in the development of inflammatory responses. A previous study has demonstrated that interactions between ICAM-1 expressed on endothelial cells and circulating monocytes may be critical for the adhesion of these cells on the vascular endothelium [7]. HIV Tat is known to exhibit diverse functional aberrations on the endothelial cells [5]. For example, Tatmediated impaired expression of adhesion molecules has been implicated as an early step in the development of cardiovascular disease associated with HIV-1-infection [5,8,9]. Although it has been documented that Tat mediated increase expression of ICAM-1 and VCAM-1 in HUVECs [4], detailed mechanisms underlying this process are not well elucidated.
MicroRNAs (miRNAs) are small RNA regulators (18-23 nucleotides) that play essential roles in a wide spectrum of biological processes [10,11]. These molecules target mRNAs on the basis of complementary sequences between the miRNAs and the 39-untranslated regions (39UTRs) of the target mRNAs, resulting in suppression of cellular target genes by inducing either mRNA degradation and/or translational suppression [11]. Because miRNAs appear to provide quantitative regulation of genes, rather than ''on-off'' decisions, they can be envisioned as fine tuners of the cellular responses to external influences [12]. miRNAs regulate many disparate processes including regulation of expression of cell adhesion molecules. Previous reports indicate the role of miR-221 in suppressing ICAM-1 translation and regulating IFN-c-induced ICAM-1 expression in human cholangiocytes [13]. However, the role of these miRNAs in the context of HIV-1 infection in HUVECs has not been yet determined.
The present study was aimed at exploring the molecular mechanisms by which Tat mediates induction of ICAM-1 in vascular endothelial cells. Understanding the regulation of ICAM-1 expression by Tat may provide insights into the development of therapeutic targets aimed at blocking inflammation in the heart of HIV-1 individuals.

Animals
For the endpoints from animal studies described herein we used 9-month old HIV transgenic male rats [HIV Tg rat, Hsd: HIV-1 (F344) Harlan] (n = 6) and 9-month old F344 male rats (as age and background-matched controls) (n = 6). HIV Tg rats express viral genes in the lymph nodes, spleen, thymus, and blood, suggesting rat cyclin T is functional with Tat; however, the animals are noninfectious due to the functional deletion of Gag and Pol within the HIV-1 provirus (thus carries only 7 of the 9 HIV genes) [15]. Rats were housed in pairs in microisolator cages within an Association for Assessment and Accreditation of Laboratory Animal Care International-approved rodent housing facility for the entirety of the study, which maintained constant temperature (20-24uC) and humidity (30-60% relative humidity). Rats had access to chow and water ad libitum throughout the study period. All animal procedures were approved by the Lovelace Respiratory Research Institute's Animal Care and Use Committee and conform to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996). All animals were euthanized by exsanguination under anesthesia (5% isoflurane by mask). Tissues were dissected and immediately snap frozen [a portion of each the heart and aorta were embedded in Tissue TekH O.C.T.
(VWR Scientific, West Chester, PA, USA) medium for histology and frozen on dry ice], and stored at -80uC until used for analysis.

Cell Culture
HUVECs, originally acquired from Lonza (Walkersville, MD), were obtained from Dr. Mukesh K. Jain's lab (Case Western Reserve University, Cleveland, Ohio, USA) and cultured in EBM-2 media as described in previous report [16].

Reverse Transcription and Real-time PCR
Total RNA was extracted with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions, as previously reported [14]. For in vivo RNA isolation endpoints, total RNA was isolated from the right superior lobe using an AllPrep DNA/RNA/protein kit (Qiagen, Valencia, CA, USA). cDNA was synthesized from total RNA in a 20 ml final reaction volume using a Verso cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, USA). The mixture was heated at 42uC for 1 h and then cooled to 4uC. Real-time PCR was performed with gene-specific primers in the ABI 7900 (Applied Biosystems, Foster City, CA, USA). The following primers were used for the Samples were run in triplicate and results for each run were averaged. DCT (change in threshold cycle) was calculated by subtracting the CT of the GAPDH control gene from the CT value of the gene of interest and mean normalized gene expression was calculated as previously described [17]. Results are expressed as normalized gene expression as percentage of GAPDH control.
For analysis of miR-221/-222, total RNA was isolated from cells with Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. Comparative real-time PCR was performed by using the TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA). Specific primers and probes for mature miR-221, -222 and snRNA RNU6B were obtained from Applied Biosystems. All reactions were run in triplicate. The amount of miR-221/-222 was obtained by normalizing to snRNA RNU6B and relative to control as previously reported [18,19].

Western Blot Analysis
Treated cells or tissue were lysed using the Mammalian Cell Lysis kit (Sigma, St. Louis, MO, USA) and the NE-PER Nuclear and Cytoplasmic Extraction kit (Pierce, Rockford, IL, USA) and quantified using the micro BCA Protein Assay kit (Pierce, Rockford, IL, USA). Equal amounts of the corresponding proteins were electrophoresed in a sodium dodecyl sulfate-polyacrylamide gel (10-12%) under reducing conditions followed by transfer to PVDF membranes. The blots were blocked with 5% non-fat dry milk in phosphate buffered saline. Western blots were then probed with antibodies recognizing the ICAM-1, ERK1/2, JNK, p38,NF-kB p65 (1:200; Cell Signaling Technology, Danvers, MA, USA) and b-actin (1:4000; Sigma, St. Louis, MO, USA). The secondary antibodies were alkaline phosphatase conjugated to goat anti mouse/rabbit IgG (1:5000). Signals were detected by chemiluminescence and imaged on the FLA-5100 (Fujifilm, Valhalla, NY, USA) digital image scanner; densitometry was performed utilizing Image J software (NIH) [20].
For monocyte adhesion, HUVECs were seeded on 96-well plates at a density of 2.5610 4 cells/well and following confluency were treated with different concentrations of Tat for 24 h as described previously [22]. In parallel, monocytes (5610 6 cells/ml) were also labeled with 10 mM cell tracker green (Invitrogen, Carlsbad, CA, USA) for 10 min, following which, monocytes were then incubated with HUVECs for 15 min at 37uC and rinsed thrice with PBS to remove the nonadherent monocytes. The fluorescence intensity of adherent monocytes was measured using a Synergy Mx fluorescence plate reader (Bio-Tek Instruments, Winooski, VT, USA).

Immunofluorescence
Sections of aorta or heart (6 mm) were incubated with 10% normal goat serum for 30 min at RT, washed in PBS, and then incubated with 250 ml per section of mouse monoclonal ICAM-1 (1:1000; Abcam, Cambridge, MA, USA) primary antibody diluted in buffer [1 part 5% blocking solution (0.5 ml Normal Rabbit Serum in 10 ml 3% w/v Bovine Serum Albumin) and 4 parts PBS] for 1 h at RT, and then rinsed 3 times with PBS. Tissue section slides were then incubated in 250 ml/per slide of secondary antibody Alexa Flour 594 (goat anti-mouse) (1:2000; Vector Laboratories, Biovalley SA, Marne la Vallée, France) with DAPI (0.5 mg/ml) in the dark for 1 h at RT. Slides were subsequently rinsed thrice in PBS, and coverslipped with Aqueous Gel Mount (Sigma, St. Louis, MO, USA). Slides were imaged by fluorescent microscopy at 10x, 40x, and 63x using the appropriate excitation/ emission filters, digitally recorded, and analyzed by image histogram, quantifying total red fluorescence, using Image J software (NIH). A minimum of 2 locations on each section (2 sections per slide), 3 slides and n = 3-5 per group were processed/ analyzed.

Statistical Analysis
Data were expressed as mean 6 standard deviation (SD) from at least three separate experiments. Significance of differences between groups was determined by Student's t test. Values of P,0.05 were taken as statistically significant.
For in vivo study data expressed as mean 6 SD. A t-test was used to compare between HIV Tg rat and F344 rat groups for real time PCR, Western blot, and immunofluorescence endpoint analysis, using Sigma Plot (v.10.0, Systat Inc., CA, USA). A p,0.05 was considered statistically significant.

Tat-mediated up-regulation of ICAM-1 in HUVECs
Since HIV-1 infection is known to alter the interaction of monocytes with human vascular endothelial cells [23] by regulating expression of adhesion molecules [6], in the present study we sought to assess the effect of Tat on the expression of cell adhesion molecules. Using flow cytometry, it was demonstrated that under normal condition HUVECs expressed almost equal levels of both ICAM-1 and activated leukocyte cell adhesion molecule (ALCAM), and lower levels of VCAM-1 (Fig. 1A). Intriguingly, activation of cells with Tat elicited a robust upregulation of ICAM-1, and a weaker induction of both ALCAM and VCAM-1 (Fig. 1A). We next sought to examine the time course of ICAM-1 induction. Tat-mediated induction of ICAM-1 mRNA in HUVECs was observed as early as 3 h following treatment and was significantly up-regulated even at 24 h posttreatment (Fig. 1B). However, heat-inactivated or mutant Tat failed to exert any significant effect on ICAM-1 expression (Fig. 1C). This finding was further confirmed by Western blot analysis demonstrating increased time-dependent expression of ICAM-1 following Tat exposure (Fig. 1D).
Since cell adhesion molecules are critical for monoctye adhesion, the next step was to examine the effect of Tat on monocyte adhesion. HUVECs were exposed to varying concentrations of Tat protein for 24 h followed by assessment of human monocyte adhesion using the in vitro assay. As shown in Fig. 1E (Fig. 1E). The concentration of Tat in the cerebral spinal fluid (CSF) has been reported to be 16 ng/ml [24]. However, the concentration of Tat in the brain remains unknown but is expected to be much higher than in the CSF [25]. The Tat concentration utilized in this in vitro study is in the physiological range [26,27]. Treatment of HUVECs with either heat-inactivated or mutant Tat did not exert any significant effect on monocyte adhesion, as expected (Fig. 1F).
Have determined the dose and time-course of Tat-mediated induction of ICAM-1, the next step was to explore the specificity of ICAM-1 in this process. HUVECs were pretreated with a neutralizing ICAM-1 antibody, followed by assessment of monocyte adhesion in the presence of Tat. In the presence of the ICAM-1 antibody, Tat failed to induce significant monocyte adhesion (Fig. 1G), thereby underpinning the role of ICAM-1 in monocyte adhesion.
Tat-induced Expression of ICAM-1 Involves MAPK (ERK1/ 2, JNK, p38) and Downstream NF-kB Pathways MAPK kinase pathways play critical roles in expression of adhesion molecules [28]. We next sought to examine the involvement of these pathways in Tat-mediated induction of ICAM-1. Treatment of HUVECs with Tat resulted in a timedependent increase in phosphorylation of MAPK ( Fig. 2A) and reciprocally, pretreatment of cells with inhibitors specific for MEK1/2 (U0126), JNK (SP600125) or p38 (SB203580) resulted in amelioration of Tat-mediated induction of ICAM-1, thereby underscoring the roles of these kinases in this process (Fig. 2B). The next step was to link Tat-mediated activation of MAPKs signaling pathways with monocyte adhesion. As shown in Fig. 2C, inhibitors specific for MAPKs blocked Tat-induced monocyte adhesion, further supporting the notion that in HUVECs sequential activation of MAPKs pathways results in up-regulation of ICAM-1 and subsequent monocyte adhesion.
Members of the NF-kB family are considered to play essential roles in ICAM-1 expression [29,30]. We therefore next  (Fig. 3A). Pretreatment of cells with IKK2 inhibitor SC514 abrogated Tat-induced ICAM-1 expression at both the mRNA (Fig. 3B) and protein (Fig. 3C) levels, thereby confirming a role for NF-kB p65 in Tat-mediated induction of ICAM-1. Moreover, we determined the role of activated NF-kB pathway in Tat-mediated adhesion of monocytes as evidenced by the fact that pretreatment of cells with SC514 blocked Tat-induced monocyte adhesion (Fig. 3D), further lending support to the notion that in HUVECs sequential activation of NF-kB pathway leads to up-regulation of ICAM-1 and subsequent monocyte adhesion.

Tat-induced Expression of ICAM-1 in HUVECs Involves miR-221/-222 Suppression
Recent studies suggest that ICAM-1 is a target of miR-221/-222, both of which regulate ICAM-1 expression in response to inflammatory stimuli [13,31]. To examine whether posttranscriptional regulation by miR-221/-222 was critical for Tat-induced expression of ICAM-1, HUVECs were treated with Tat and assessed for expression of miR-221/-222 by real-time PCR. Following Tat-mediated stimulation of HUVECs, there was a significant decrease in the expression levels of both miR-221/-222 in HUVECs following Tat stimulation for 12 h, as assessed by real-time PCR (Fig. 4A).
Next, in order to test the role of miR-221/-222 in Tat-mediated induction of ICAM-1, HUVECs were transfected with miR-221/- 222 precursors for 24 h, followed by exposure to Tat for 12 h, with subsequent assessment of ICAM-1 protein expression by Western blot. As shown in Fig. 4B and 4C, precursors of both miR-221/-222 significantly blocked Tat-induced ICAM-1 protein expression in HUVECs. Transfection of cells with a precursor control sequence, however, did not inhibit Tat-mediated induction of ICAM-1.

Tat-mediated Decrease of miR-221/-222 Expression Involves NF-kB Pathway
Having determined the role of NF-kB in Tat-induced expression of ICAM-1, we next sought to examine its role in Tat-mediated down-regulation of miR-221/-222. Cells were pretreated with SC514 for 1 h followed by additional incubation for 12 h in the presence or absence of Tat and assessed for expression of miR-221/-222 by real-time PCR analysis. A significant decrease in miR-221/-222 levels was detected in Tat-treated HUVECs, which was significantly ameliorated in cells pretreated with SC514 (Fig. 5A, B).

MiR-221/-222 Regulated Expression of ICAM-1 Plays a Role in Monocyte Adhesion
Over-expression of adhesion molecule such as ICAM-1 on endothelial cells is known to directly contribute to increased monocyte adhesion on the endothelium. In order to determine the functional relevance of miR-221/-222-regulated expression of ICAM-1 in HUVECs, monocyte adhesion assays were performed in the presence of HUVECs transfected with miR-221/-222 precursors. As shown in Fig. 6A and B, there was significant reduction of monocyte adhesion in cells transfected with either of the precursors. These data further corroborate that Tat-mediated suppression of miR-221/-222 is involved in expression of ICAM-1 in HUVECs and also influences monocyte adhesion. inhibitor SC514 (10 mM). HUVECs were exposed to Tat for 12 h followed by cell adhesion assay. All the data are presented as mean 6 SD of three independent experiments. **p,0.01 vs control; ## p,0.01 vs Tat-treated group. doi:10.1371/journal.pone.0060170.g003

Up-regulation of ICAM-1 Expression in the Aorta from HIV Transgenic (Tg) Rats
To validate the cell culture findings in vivo, next step was to examine expression of ICAM-1 RNA and protein both in isolated aorta as well as the heart tissues isolated from HIV Tg rats that express 7 of the 9 HIV proteins including Tat. As shown Fig. 7A, there was a significant increase in expression of ICAM-1 mRNA in the aorta of HIV Tg rats compared with the wide type (WT) animals. In the heart of Tg animals, while there was a trend towards increased ICAM-1 mRNA expression compared with the WT rats, the difference was not statistically significant. Immunofluorescence findings herein thus suggest that in the hearts of HIV transgenic rats, ICAM-1 is up-regulated in the endocardium (endothelial cells), leading to functional consequences of increased monocyte adherence in the aorta/heart. Intriguingly, homogenates of the aorta and heart also demonstrated increased expression of ICAM-1 when assessed by real-time RT-PCR and Western blot as shown in Figs. 7B and C. Furthermore, consistent with the in vitro findings, expression of miR-221, but not miR-222, was decreased in aorta isolated from HIV Tg rats, compared with the WT rats (Fig. 7D).

Discussion
Numerous studies have shown that HIV infection is an independent risk factor for cardiovascular disease [2,32,33]; however, the mechanism(s) by which HIV-1 induces cardiovascular disease (CVD) are not well understood and are likely multifactorial. HIVCM are accompanied by traditional cardiovascular risk factors, including endothelial dysfunction, chronic inflammation, lipid disorders, as well as proposed direct HIV-1 infection of the endothelium [34,35,36]. Additionally, several HIV viral proteins have been implicated in causing HIV-associated  [36,37,38]. However, the detailed mechanisms underlying these processes remain unclear.
Adhesion molecules such as ICAM-1 are known to mediate leukocyte (and platelet)-endothelial cell interactions in the vasculature under both physiological and pathological conditions. Importantly, ICAM-1 has been shown to play an essential role in the development of vascular inflammation and endothelial dysfunction [39] that is associated with the pathogenesis of multiple cardiovascular diseases including atherosclerosis, coronary artery disease, myocarditis, and hypertrophic cardiomyopathy [40,41]. In the present study, it was demonstrated that exposure of HUVECs to Tat leads to the induction of both ICAM-1 mRNA and protein. To delve deeper into the molecular mechanisms of Tat-mediated induction of ICAM-1 expression, the intercellular signaling events such as MAPK kinases were assessed.
Using both the pharmacological and genetic approaches, we examined the activation of MAPK kinase pathways in Tatmediated induction of ICAM-1. These findings are consistent with the previous reports implicating the role of these signaling pathways in the induction of ICAM-1 [30,42]. MAPKs followed by the activation of NF-kB participate in the intracellular signal transduction and production of adhesion molecules in various cell systems [30,43]. Consistent with these findings, the role of MAPKs and p65/RelA nuclear translocation were also demonstrated in Tat-mediated induction of ICAM-1expression.
Recent years have seen a splurge in publications on the gene regulation by miRNAs, small RNA regulators that play essential roles in disparate biological processes [10,11]. Recent reports indicate the role of miR-221/-222 in suppressing ICAM-1 expression [13,31]. In the present study, for the first time, we report the role of Tat in modulating the regulation of ICAM-1 via miR-221/-222 in HUVECs. We have demonstrated that the downregulation of miR-221/-222 involves activation of NF-kB. These findings complement previous studies on the role of miR-221/-222 in suppression for ICAM-1 translation, as evidenced by the fact that miR-221/-222 targeted ICAM-1 39UTR and suppressed ICAM-1 translation [13]. Further confirmation of the role of miR-221/-222 was carried out by overexpressing miR-221/-222, which significantly decreased Tat-induced monocyte adhesion, underpinning the role of miR-221/-222 in regulating of ICAM-1 expression with concomitant monocyte adhesion.
Functional significance of Tat-induced ICAM-1 was examined in an in vitro model of cell adhesion. Treatment of HUVECs with Tat resulted in increased monocyte adhesion, and this effect was significantly inhibited by pre-treating cells with the neutralizing antibody specific for ICAM-1, thereby underpinning the role of ICAM-1 in monocyte adhesion induced by Tat. Further validation of these cell culture findings was carried out in vivo in a wellestablished HIV transgenic rat model that expresses 7 of the 9 HIV genes including Tat. Intriguingly, a recent report has demonstrated increased cardiomyopathy in HIV Tg animals compared to the WT controls as evidenced by elevated right ventricular systolic pressure and right ventricular hypertrophy [44]. Based on this as well as on our cell culture findings that Tat induced ICAM-1 expression in HUVECs, we sought to examine the expression of ICAM-1 protein both in the aorta as well as the heart tissue of these adult HIV Tg rats. The rationale behind this being that the accumulation of Tat in these adult animals could lead to increased ICAM-1 expression in the heart and aorta of these animals, thus leading to cardiac complications. As shown there was a significant elevation in ICAM-1 RNA and protein expression in the aorta, and to a lesser degree in the hearts of HIV-1 Tg rats, compared with the WT controls. Interestingly, immunofluorescence staining of the hearts revealed a notable increase in ICAM-1 expression specific to the endothelial layer (endocardium) of the heart of the HIV Tg rats. Furthermore, similar to our cell culture findings there was a significant decrease in the RNA expression of miR-221 in the aortas isolated from HIV Tg rats compared with the WT controls. While miR-222 RNA was also downregulated in the aortas of Tg rats, the reduced level of expression was not significant compared to controls. Taken together these findings underpin the role of miR-221 in regulating expression of ICAM-1. These findings are in concordance with a similar report identifying the role of miR-221 in suppression of ICAM-1 translation [13].
In summary, our findings have identified a detailed molecular pathway of Tat-mediated induction of ICAM-1, involving activation of MAPK pathways leading to translocation of NF-kB into the nucleus and ultimately resulting in increased ICAM-1. Furthermore, Tat-mediated expression of ICAM-1 played a vital role in monocyte adhesion, leading to increased recruitment of inflammatory cells onto the vessel endothelium. These findings not only have implications for HIV-1-infected individuals that have an increased risk of CVD, but also infected individuals that are treated with ART. In this latter population, increased longevity due to ART usage has resulted in increased prevalence of CVD, which is becoming an increasingly important health burden [45,46,47,48]. In light of our findings and the published report that exposure of human aortic endothelial cells to combinations of protease inhibitors and nucleoside reverse transcriptase inhibitors resulted in increased expression of ICAM-1 VCAM-1, and endothelial-leukocyte adhesion molecule [49], it can be envisioned that HIV infection and ART together can lead to higher risk of development of CVD. Therapies provided to infected patients must thus be administered with caution to avoid drug-mediated effects on CVD. Figure 6. Overexpression of miR-221 or miR-222 inhibits monocyte adhesion induced by Tat in HUVECs. Transfection of HUVECs with miR-221 or miR-222 precursor abrogated Tat-induced monocyte adhesion. HUVECs were transfected with miR-221 or miR-222 (50 nM) precursors for 24 h, then exposed to Tat (14.4 nM) for 12 h followed by monocyte adhesion assay. All the data are presented as mean 6 SD of three independent experiments. **p,0.01 vs control; ## p,0.01 vs tat-treated group. doi:10.1371/journal.pone.0060170.g006