Willin, an Upstream Component of the Hippo Signaling Pathway, Orchestrates Mammalian Peripheral Nerve Fibroblasts

Willin/FRMD6 was first identified in the rat sciatic nerve, which is composed of neurons, Schwann cells, and fibroblasts. Willin is an upstream component of the Hippo signaling pathway, which results in the inactivation of the transcriptional co-activator YAP through Ser127 phosphorylation. This in turn suppresses the expression of genes involved in cell growth, proliferation and cancer development ensuring the control of organ size, cell contact inhibition and apoptosis. Here we show that in the mammalian sciatic nerve, Willin is predominantly expressed in fibroblasts and that Willin expression activates the Hippo signaling cascade and induces YAP translocation from the nucleus to the cytoplasm. In addition within these cells, although it inhibits cellular proliferation, Willin expression induces a quicker directional migration towards scratch closure and an increased expression of factors linked to nerve regeneration. These results show that Willin modulates sciatic nerve fibroblast activity indicating that Willin may have a potential role in the regeneration of the peripheral nervous system.


Introduction
The Salvador/Warts/Hippo (Hippo) signaling pathway controls tissue growth and organ size by promoting a normal fine tuned homeostasis. Originally characterized in Drosophila melanogaster, this pathway limits overgrowth, which may lead to cancer development by inhibiting cell proliferation and promoting apoptosis [1][2][3][4]. Highly conserved, the Drosophila Hippo pathway core components and downstream effectors have direct orthologues in mammals. This functional conservation is confirmed by the ability of mammal orthologues to rescue Drosophila mutants in vivo; the core components Hpo, Sav, Wts and Mats have respective vertebrate homologues MST1 and MST2, WW45 (also known as Sav1), the kinases LATS1 and LATS2 and MOB1 (MOBKL1A/ B). The main targets of the mammalian Hippo signaling cascade are the two Drosophila Yki orthologues, Yes-associated protein (YAP) and the transcriptional coactivator with PDZ binding motifs (TAZ). Mimicking the regulation process observed in Drosophila Yki when in the presence of high cell densities, LATS phosphorylates YAP at Ser127 and TAZ at Ser89 creating a protein 14-3-3 binding site, which in turn leads to YAP/TAZ cytoplasmic localization. Defects in the Hippo pathway lead to a lack of YAP/TAZ cytoplasmic sequestration which results in YAP/TAZ nuclear accumulation and, eventually, in tumour development [5][6][7][8] Indeed, we and others have previously described that deregulation of the Hippo signaling cascade components results in YAP dephosphorylation, nuclear sequestration and overgrowth [4,[9][10][11][12][13][14][15]. Less is known about the upstream components of the core kinase cassette. Merlin, KIBRA (WWC1) and Willin (FRMD6) have been shown to be upstream of MST1/2 and to be able to induce LATS and YAP phosphorylation [9,11,[16][17][18][19]]. Yet, so far only Merlin is functionally validated in vivo, although recently we showed that reduced KIBRA expression in primary breast cancer specimens correlates with the claudin-low subtype [11].
Several lines of research have established a new role for the Hippo signaling pathway in tissue regeneration. Tissue repair, after a major injury, relies on the expansion and/or dedifferentiation of an existing population of progenitor cells, such as primary satellite cells [20] which may recapitulate the developmental process involving the reprogramming of diverse molecular mechanisms [21]. Here, repression of pathways involved in developmental tissue growth, such as the Hippo kinase cascade, induces YAP stemness properties by promoting a controlled cell proliferation aiming restore of organ function [22,23]. Regeneration of the peripheral nerve is a dynamic process initiated by Schwann cells and the extracellular matrix, which confers a growth promoting environment to peripheral axons. The signal transmitted by damaged axons to Schwann cells informing them of their intention to degenerate is mediated by Raf/MEK/ERK signaling pathway and triggers myelinated Schwann cells to dedifferentiate to a progenitor-like cell [24,25]. Regrowing axons and Schwann cells migrate towards the nerve gap to promote reinnervation of the distal stump, and relies on signals mediated by both the ephrin/Eph pathway and fibroblasts [26].
Willin (FRMD6) was first identified in the rat sciatic nerve [27] which is composed of neurons, Schwann cells and fibroblasts, and is the nearest human sequence homologue to the Drosophila protein Expanded (Ex) sharing 60% of homology with the Ex FERM domain. In Drosophila, the lack of Ex expression has been shown to be associated with overgrowth of certain structures such as the wing and imaginal discs [28] reflecting a direct role in controlling cell growth in these tissues. In mammals, recently the FERM domain of Willin was shown to be sufficient to activate the Hippo pathway via MST1/2 and to antagonize YAP-induced phenotypes in mammalian cells [9]. Willin activated the Hippo pathway, inducing the phosphorylation of MST1/2, LATS1 and YAP in MCF10A and 293T cells. Knockdown of Willin expression mimicked YAP overexpression with respect to inducing an Epithelial-Mesenchymal transition (EMT) phenotype in MCF10A cells [9]. As Willin was first identified within the mammalian sciatic nerve, we sought what its functions could be within this structure and whether it shares the same functionalities as in epithelial cells. We show that in mammalian peripheral nerve fibroblasts Willin expression can control the phosphorylation of MST1/2, LATS1 and YAP and thereby YAP's translocation from the nucleus to the cytoplasm. We also demonstrate that activation of the Hippo pathway is an upstream regulator of ephrinB/EphB signaling and EGFR, which are known factors to be involved in nerve regeneration, and in addition we show that Willin expression can control the rate at which wounds may close. Therefore our results demonstrate that Willin and the Hippo kinase cassette play a role in the maintenance of peripheral nerve system homeostasis.

Schwann Cells and Fibroblasts Purification
Schwann Cells (SC) were purified using a modification of the method described by Brockes and colleagues [29]. All procedures were carried out in accordance with the guidelines, set forth by the Animals Scientific Procedures Act under schedule 1 procedures. Mice had access to food and water, ad libitum. Furthermore, all procedures were carried out in accordance with the guidelines, set forth by the Animals Scientific Procedures Act, under a project license (No. 6003895) granted by the UK Home Office and with the approval of the University of Glasgow Ethical Review Process Applications Panel. Briefly, sciatic nerve was dissected from neonatal P7 Sprague-Dawley pups. The tissue was minced finely and enzymatically digested with trypsin (100 ml of 2.5%, Invitrogen) and collagenase 500 ml collagenase (1.33%; ICN Pharmaceuticals). The mixed cell population was cultured in 10% FBS without specific SC mitogens for approximately 48 hrs before the addition of cytosine arabinoside (AraC, 10 25 M, Sigma, Dorset, UK) for a further 48 hrs to eliminate rapidly dividing fibroblasts. Further purification was then carried out by trypsinising the cells and resuspending them in a small volume of serum free media with anti-Thy1.1 antibody at room temperature for 15 min (1:50 supernatant, Sigma, Dorset, UK), followed by the addition of rabbit complement (1:4, Harlan Laboratories Ltd., UK) for 45 min at 37uC [30]. All cell cultures were grown in PLL coated tissue culture flasks. To isolate fibroblast from sciatic nerve a culture was set up as described above but the fibroblasts were not removed by the Thy1.1 complement-mediated kill. The cultures were fed with 10% FBS and the fibroblast over grew the Schwann cells.

Plasmid Construction
The human Willin-HA ORF was cloned into the pBABEpuro vector (pBabe-puro, Addgene, Cambridge, MA, USA) as an BamHI-EcoRI fragment. The human pBABE-YAP1-Flag (Addgene plasmid 15682) expression clone was described previously (Overholtzer et al., 2006;Angus et al., 2012). pBABE constructs were packaged in PhoenixA cells for viral production. Retroviral infection of primary fibroblasts was performed accordingly standard protocols. The transduced cells were selected, after 24hours, with 2.5 mg/mL puromycin or with 300 mg/mL hygromycin. The selections continued for 5 days (puromycin) or 10 days (hygromycin) after which cells were harvested for downstream experiments. siRNA targeting Willin/FRMD6 (ID #s141256) and a non-targeting siRNA control (ID #4390844

In situ Hybridization
In situ hybridization was performed with cRNA probes on sciatic nerves of 21 day old mice as previously described [31]. Briefly, RNA was prepared by TRIZOL extraction (Life Technologies) for RT-PCR. Reverse transcription was performed with 1 mg of RNA using SuperScriptTMII reverse transcriptase (400 U; Life Technologies) in the presence of random hexamers (Life Technologies). After incubation at 42uC for 45 min, reactions were terminated by heating at 80uC. DNA in 1 ml of the first strand reaction was amplified by PCR with Dynazyme (Flowgen). Primer sequences were selected from the published neurofascin (ankyrin-binding protein) sequence (available from Gen-Bank/EMBL/DDBJ under accession No. L11002) [32], that would specifically amplify product from the 155-splice variant of neurofascin. A probe specific for NF155 was amplified from within the third FNIII domain of neurofascin with a forward primer, 59-CCTGAACAG-CACAGCCATCAG-39 (nucleotides 3,550-3,570) and a reverse primer 59-GACCACAACCATCTCCTGCTTG-39 (nucleotides 3,775-3,754). The products were cloned into the pGEM-T vector (Promega) and sequenced. The cDNA templates were linearized with either NotI or NcoI and transcribed with either T7 (antisense probes) or SP6 RNA (sense probes) polymerases (Boehringer Mannheim), respectively. Probes specific for Willin were made using the plasmid pSPORT-Willin using the T7 promoter for

Nuclear/Cytoplasmic Fractionation
Primary fibroblasts subcellular fractionation was performed using a modification of the method described by Angus and colleagues [9]. Briefly, after reached the desired confluence cells were harvested, pelleted at 228 g rpm for 4 min, washed with PBS and pelleted again at 228 g for 4 min. Pellet was resuspended in 0.5 ml of ice-cold cytoplasmic extraction buffer (10 mM HEPES pH 7.9, 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM DTT and cocktail of protease inhibitors) and kept on ice for 5 min. Cells were lysed with 20 strokes using a pre-chilled Dounce homogenizer and the lysate was centrifuged at 228 g for 5 min at 4uC. The pellet (nuclear fraction) was resuspended in 1 ml of ice-cold nuclear extraction buffer (0.25 mM sucrose, 10 mM MgCl 2 and cocktail of protease inhibitors), layered over a sucrose bed (0.88 mM sucrose, 0.5 mM MgCl 2 and cocktail of protease inhibitors) and centrifuged for 10 min at 4uC. Pellet was resuspended in 500 ml of 1x Fibro-vector vs Fibro-Willin: *p,0.05; **p,0.01; ***p,0.001; Student's t-test. (B) Willin knockdown induces YAP, LATS1 and MST1/2 dephosphorylation. Primary fibroblasts were transfected with either non-targeting siRNA (siCtr) or siRNA targeting Willin (siWillin) for two consecutive days. Cell lysates for western blotting analysis were prepared 48 h post-transfection. The ratios between relative YAP, LATS1 and MST1/2 phosphorylation levels to total proteins (YAP, LATS1 and MST1/2) are shown and background phosphorylation of Fibro-siCtr was arbitrarily set to 1. Immunoblot analysis of efficient Willin knockdown is also presented. b-actin was used as a loading control. Means were calculated from three independent experiments. Error bars represent 6 s.e. (n = 3). Fibro-siCtr vs Fibro-siWillin: *p,0.05; **p,0.01; ***p,0.001; Student's t-test. (C) Willin expression results in YAP, but not TAZ, translocation from the nucleus to the cytoplasm. Cytoplasmic and nuclear fractions were separated for western blot analysis as indicated. The blots shown are representative of three independent experiments (n = 3). (D) Willin knockdown in primary fibroblasts sequesters YAP, but not TAZ, in the nucleus. YAP and TAZ subcellular location was determined by immunoblotting analysis of cytoplasmic and nuclear fractions. b-actin and Lamin-b were used as loading controls for the cytoplasmic and nuclear fractions, respectively. The blots shown are representative of three independent experiments (n = 3). doi:10.1371/journal.pone.0060028.g002 RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 0,5% sodium deoxycholate and cocktail of protease inhibitors), briefly sonicated on ice and centrifuged at 2800 g for 5 min at 4uC. The supernatant (cytoplasmic fraction) was transferred to a new 1.5 ml tube, 300 ml of 1x RIPA buffer was added and the lysate centrifuged at 2800 g for 10 min at 4uC to pellet any solids. The supernatant was transferred to a new tube. Protein sample buffer was added to both nuclear and cytoplasmic fractions and 30 mg of protein lysates run on an SDS/10% polyacrylamide gel.

In vitro Wound Healing Assay
For both overexpression and knockdown analysis the same number of cells were plated in each well of a 6-well culture plate (8610 5 and 3610 5 , respectively). For the knockdown experiments, cells at 60% confluence were transfected on two consecutive days with siFRMD6 or siCtr and allowed to grow until reaching confluence (3 days after the initial transfection). Monolayers of confluent cultures were gently scratched with a sterile micropipette tip and the migration towards the wound was monitored for up to 16 h (overexpression) or 40 h (knockdown). Phase-contrast images were captured after the scratch for each one of the time points. The percentage of scratch covered by cells was measured as the percentage of the invaded area with respect to the initial wound area and calculated using Image J software.

BrdU/PI labeling and Flow Cytometry
For cell cycle progression analysis, cells were cultured until desired confluence and pulse-labeled with 10 mM BrdU (Sigma-Aldrich, Dorset, UK) for 1hour at 37uC. After trypsinization and two PBS/1% BSA washes cells were fixed in 70% ethanol at 4uC for at least 30 min, centrifuged at 500 g for 10 min at 10uC and the pellet was resuspended in 2 N HCl for 30 min on ice for DNA denaturation. Cells were centrifuged at 500 g for 10 min at 10uC and washed with 0.1 M of sodium tetraborate pH 8.5, centrifuged again and stained with anti-BrdU (# 347580, BD Biosciences, Oxford, UK) for 1 hour, room temperature, in the dark. Cells were then washed and stained with Dylight 488-goat anti-mouse IgG (#115-485-062, Stratech, Newmarket, UK) for 1hour at RT. Stained cells were resuspended in PBS containing 20 mg/ml of propidium iodide (Sigma-Aldrich, Dorset, UK) and RNase at 100 mg/ml for 30 min at RT. For cell cycle analysis using propidium iodide, cells were washed twice in ice-cold PBS, fixed in 70% ethanol at 4uC for at least 30 min, washed twice in ice-cold PBS and then resuspended in PBS containing 4 mg/ml of propidium iodide and RNase at 100 mg/ml for 30 min at RT, in the dark. Cells were then analysed on a FACScan (BD Biosciences, Oxford, UK) using CELLQUEST software.

Willin is Expressed in Fibroblasts within the Sciatic Nerve
Willin was first identified by a yeast two-hybrid screen of a rat sciatic nerve cDNA library using neurofascin as bait and northern blot analysis had confirmed Willin's expression in this structure [27]. To establish where Willin is located within the sciatic nerve, we compared the mRNA expression pattern of Willin with the Schwann cell expressing isoform of the transmembrane receptor neurofascin [33] by in situ hybridization. The Willin transcript appeared to be predominately located at the perineurium (the periphery) of the sciatic nerve ( Figure 1A), but also within discrete cells within the endoneurium. However, from their morphology these cells appeared to be different from those in which the glial neurofascin isoform transcript was expressed ( Figure 1A). The sciatic nerve is composed of cell bodies of Schwann cells and fibroblasts; therefore, to determine the expression of Willin in either of these cells, both Schwann cells and fibroblasts were cultured from rat sciatic nerve ( Figure 1B). From these cultures, quantitative RT-PCR demonstrated that the Willin transcript was expressed 10-fold more in the fibroblasts than in the Schwann cells ( Figure 1C). Since Willin is an upstream component of the Hippo pathway [9,11] components of this newly emerging signaling cascade were also analyzed to determine whether they were expressed within the sciatic nerve. Interestingly, MST2 and LATS1 were more strongly expressed within the fibroblasts than Schwann cells, whilst YAP was less expressed and MST1 was present in both cell types at the same level as assessed by quantitative RT-PCR ( Figure 1C).

Willin Expression Influences the Activation of the Hippo Pathway in Fibroblasts
Previously, it has been shown that Willin expression in epithelial MCF10A cells induces YAP phosphorylation at Ser127, by a MST1/2 and MOBKL1A/B-dependent mechanism [9,11]. Phosphorylation of YAP at Ser127 by LATS1/2 results in YAP translocation to the cytoplasm and then its subsequent ubiquitination and concomitant degradation [4,8]. Since YAP regulation could be context-dependent, Willin was investigated to determine whether it could activate the Hippo pathway in primary fibroblasts derived from the rat sciatic nerve. Primary fibroblasts were infected with retroviruses expressing either Willin or an empty vector, and stable pools of cells were selected. Upon ectopic Willin expression (Fibro-Willin cells), as predicted there was a significant increase in MST1/2, LATS1 and YAP phosphorylation when compared to the empty-vector control (Fibro-Vector) cells (Figure 2A). These findings were further supported by knocking down endogenous Willin using RNA interference. Down-regulation of Willin using siRNA (Fibro-siWillin cells) caused a significant reduction in MST1/2, LATS1 and YAP phosphorylation ( Figure 2B), when compared with control cells (Fibro-siCtr). Knockdown of Willin was confirmed by immunoblotting analysis ( Figure 2B). Furthermore, lysates from Fibro-Willin or Fibro-Vector cells were separated into cytoplasmic and nuclear fractions. Efficient phosphorylation of YAP at Ser127 induces cytoplasmic retention through binding to 14-3-3 proteins [8]. As expected, Willin expression promoted YAP sequestration in the cytoplasm. Surprisingly, the localization of TAZ, the paralogue of YAP, was unaffected by Willin expression ( Figure 2C). Moreover, investigations were also carried out to determine whether YAP dephosphorylation was accompanied by YAP sequestration in the with either non-targeting siRNA (siCtr) or siRNA targeting Willin (siWillin) for two consecutive days. Images were taken at low and high density. At low density, siWillin fibroblasts present a large and flat spindle-shape with multiple oval nucleoli with extended and interconnected cell processes protruding from the body of each cell. At high density, the cells are confluent and arranged in parallel arrays with the interconnected processes organized in a dense and close network. This effect is not observed in Fibro-siCtr cells. (F) Proliferation curve of control fibroblasts (siCtr) or knockdown Willin (siWillin) cells. Cells were transiently transfected with the respective siRNAs and the growth curve monitored over 4 days. 48  nucleus upon Willin knockdown. Indeed, YAP nuclear retention was observed upon Willin knockdown (Fibro-siWillin cells) when compared with control cells (Fibro-siCtr). However, TAZ consistently did not translocate from the cytoplasm to the nucleus in this scenario ( Figure 2D). Taken together, the data suggest that within sciatic nerve fibroblasts, Willin is an upstream regulator of YAP but not TAZ.

Willin Suppresses Cellular Proliferation in a Cell-cycle Independent Manner
To explore the effect of Willin and YAP expression on cell proliferation, primary fibroblasts were infected with retroviruses expressing Willin, YAP1 (Fibro-YAP) or an empty vector. Stable pools of cells were selected and equal numbers of cells were cultured and counted every day for 6 days ( Figure 3A). Fibro-Willin cells showed a significant delay in proliferation when compared to Fibro-YAP or Fibro-Vector cells. Interestingly, ectopic YAP expression resulted in increased cellular proliferation, when compared with Fibro-Vector cells ( Figure 3A). Ectopic expression of Willin and YAP was confirmed by immunoblotting analysis ( Figure 3B).
To determine if the slower proliferation rate of Fibro-Willin cells was associated with cell cycle changes, these cells were analyzed by flow cytometry. Fibro-Willin, Fibro-YAP or Fibro-Vector cells were seeded at 1610 6 cells (corresponding to day 2 of Figure 3A) and 24 h later they were pulse-labeled with BrdU for 1 hour and probed by flow cytometry. There was no statistically significant difference in the number of cells residing in G0/G1, S or G2/M phases ( Figure 3C) in any of the cell-types tested, suggesting that there was no cell cycle arrest in Fibro-Willin cells.
Complementing the results observed in the overexpression scenario ( Figure 3C), cells with decreased Willin expression (Fibro-siWillin), displayed no statistically significant difference in the number of cells observed in G0/G1, S or G2/M phases compared to Fibro-siCtr cells ( Figure 3D). However, the morphology of Fibro-siWillin cells was notably different: at low density, the Fibro-siWillin cells had elongated cell processes and a more pronounced flat spindle shape compared to Fibro-siCtr cells; after three days, Fibro-siWillin cells organized into a dense and close network of multiple interconnected processes protruding from the body of each cell ( Figure 3E). This effect was accompanied by a statistically significant 3.5 fold increase in cellular proliferation compared to Fibro-siCtr cells ( Figure 3F). These observations suggest that in fibroblasts Willin expression negatively regulates cellular proliferation but in a cell cycle independent manner.

Willin Expression Promotes Fibroblast Migration
Fibroblasts have an important role in wound healing, since these cells are the first to bridge the physical gap between the proximal and distal stumps following sciatic nerve transection [34,35]. To assess the effect of Willin and YAP expression in promoting fibroblast wound closure, scratch assays were performed on confluent fibroblast monolayers and the rate of scratch closure was observed over 16 h. Strikingly, Fibro-Willin cells demonstrated a faster scratch closure rate compared to Fibro-Vector cells ( Figure 4A). Cell numbers were counted to determine whether the faster scratch closure by Fibro-Willin cells was due to increased cellular proliferation or directional cell migration towards the wound. There was no change in the number of Fibro-Willin cells over the time course of the experiment in contrast to Fibro-YAP cells which showed increased cellular proliferation after 16 h compared to Fibro-Vector cells ( Figure 4B). The impact of Willin on fibroblasts migration was further confirmed using Fibro-siWillin cells. As predicted, Fibro-siWillin cells displayed an inhibition of migration when compared to Fibro-siCtr cells ( Figure 4C). Taken together, the results suggest that Willin expression promotes the migration of sciatic nerve derived fibroblasts.

Willin is an Upstream Regulator of EphrinB2 and EGFR Expression
Previously, Parrinello and coworkers described ephrin/Eph signaling from fibroblasts is responsible for a directional axonal outgrowth of Schwann cells [26]. In order to establish whether the Hippo pathway plays a role in ephrin/Eph signaling, the expression of ephrinB2 was analyzed in Fibro-Willin, Fibro-YAP and Fibro-Vector cells. Quantitative RT-PCR ( Figure 5A) and immunoblotting analysis ( Figure 5B) showed that ectopic Willin expression resulted in ephrinB2 down-regulation whereas YAP induced ephrinB2 up-regulation as compared to Fibro-Vector cells. This finding was complemented by determining whether endogenous Willin regulates ephrinB2 expression. As predicted, knockdown of Willin in Fibro-siWillin cells resulted in an increase in ephrinB2 levels, both at mRNA ( Figure 5C) and protein levels ( Figure 5D), as compared to Fibro-siCtr cells.
These results were also closely mirrored when levels of the epidermal growth factor receptor (EGFR) were also monitored as previous studies have shown that activated EGFR leads to increased cell migration in wound repair mediated by fibroblasts [36][37][38]. Notably, there was a down-regulation of EGFR in Fibro-Willin cells compared to Fibro-Vector cells, both at the mRNA ( Figure 5A) and protein levels ( Figure 6B). Conversely, comparing the Fibro-YAP cells to the control Fibro-Vector cells resulted in a significant increase in EGFR mRNA levels ( Figure 5A) but a nonsignificant increase at the protein level ( Figure 5B). Confirming the role of Willin on EGFR expression, Fibro-siWillin cells displayed a predicted up-regulation of EGFR as compared to Fibro-siCtr cells, both at the mRNA ( Figure 5C) and protein level ( Figure 5D). Wound closure ability of fibroblasts stable cells was assessed by a wound-healing assay. Representative phase contrast images show the wounded area (0 h) and cell migration towards the wound after 14 h and 16 h. The percentage of scratch covered was measured by quantifying the total distance the cells moved from the edge of the scratch towards the center of the scratch (shown by red lines), using Image J software, followed by conversion to a percentage of scratch covered. Data is presented as the mean percentage of scratch covered in three independent experiments. Error bars represent 6s.d. Previously, the Willin FERM domain was shown to be sufficient to influence the activity of genes regulated by YAP [9]. Since the Hippo kinase cascade has cell-specific regulatory functions in different tissues [4,13] some of these genes were analyzed to determine whether they are influenced by Willin and YAP in sciatic nerve fibroblasts. To test this, quantitative RT-PCR analysis of CTGF, BMP2, FGF1, RASSF8, IGFBP3 and PRL mRNA levels was conducted on Fibro-Willin, Fibro-YAP and Fibro-Vector cells ( Figure 6). PRL (prolactin), among all the analyzed genes was the most strongly up-regulated by 5-fold in Fibro-Willin cells when compared to Fibro-Vector cells, but downregulated in Fibro-YAP cells. Intriguingly, CTGF, a direct YAP target gene in NIH-3T3 and MCF10A cells [39] was downregulated in Fibro-YAP cells as compared to Fibro-Vector cells. Loss of CTGF expression was also observed in Fibro-Willin cells as compared to Fibro-Vector cells. Notably, FGF1 and IGFBP3 mRNA levels were significantly up-regulated in Fibro-Willin cells and down-regulated in Fibro-YAP cells, when compared to Fibro-Vector cells. Furthermore, BMP2 and RASSF8 were up-regulated in Fibro-Willin cells when compared to Fibro-Vector cells. These data demonstrate that, in fibroblasts isolated form the sciatic nerve, Willin and YAP have antagonistic regulatory functions upon FGF1, RASSF8, IGBP3 and PRL genes, but other genes show context-dependent regulation such that BMP2 expression is influenced by Willin and not YAP, while CTGF expression is inhibited by both Willin and YAP.

Discussion
Although several advances have been made in the understanding of the emerging mammalian Hippo signaling pathway in its regulation of organ size control, tissue regeneration and stem-cell renewal [4,13] little is known about the involvement of this pathway in the peripheral nervous system where Willin/FRMD6 was first identified [27]. This study establishes a role for Willin in mammalian peripheral nerve fibroblasts based on four findings. Firstly, we find that Willin as well as other components of the Hippo signaling pathway are expressed in these fibroblasts. Secondly, Willin has previously been shown to activate the Hippo pathway in epithelial cells [9,11] and we extend this to fibroblasts, where Willin expression increases MST1/2, LATS1 and YAP phosphorylation, and consequently a shift of YAP from the nucleus to the cytoplasm. These findings were further confirmed by knockdown of Willin. Thirdly, we show that Willin expression promotes fibroblast wound closure by directional migration in the absence of proliferation. Fourthly, we show Willin expression down-regulates ephrinB2 and EGFR (Figure 7).
Fibroblasts are the main mediators of the dynamic and wellorganized process of wound healing. They accumulate at the injury site [26], synthesize ECM components forming granulation tissue, and promote mechanical forces within the wound to initiate tissue contraction, a mechanism that leads to scar formation [40]. Fibroblasts are also involved in angiogenesis, promoting wound vascularization and inflammation by secreting proinflammatory cytokines [41]. If extensive invasion and proliferation of scarforming fibroblasts occurs, pathophysiological conditions may arise such as neuromas or hypertrophic scars [42]. During the process of tissue repair, extracellular matrix goes through physical changes in terms of elasticity and cell shape. Recently YAP has been described as a crucial mediator of physical and mechanical cues in the cellular microenvironment, such that a ''stiff'' microenvironment activates YAP [43,44]. Therefore, these modifications may exert mechanical signals that activate YAP, which would be antagonized by signals from the Hippo pathway.
Willin expression induces wound closure in the absence of proliferation and this might be associated with an initial injury event. When the microenvironment becomes more ''stiff'' upon the accumulation of fibroblasts, then YAP activation would predominate, increasing fibroblast proliferation, inhibiting fibroblast migration and causing increased Ephrin B2 and EGFR expression in the fibroblasts. Parrinello and coworkers (2010) [26] recently described that ephrinB2 ligand expression on fibroblasts induces activation of the EphB2 receptors located on Schwann cells. The ephrinB2/EphB2 signal results in an organized directional cell migration by the Schwann cells as it mediates their sorting in the form of multicellular cords to guide axons regrowth across the wound. Activation of EphB2 receptor on Schwann cells was found to be Sox2 dependent [26]. Sox2 is pivotal for the maintenance of pluripotency and regulation of stem cell self-renewal and differentiation [45,46]. Interestingly, YAP has been shown to regulate Sox2 in mES cells [23] and we observed higher YAP expression in Schwann cells as compared to fibroblasts. Without an antagonizing signal this process would continue resulting in an excess of fibroblasts. In fact, ephrinB2 has been found overexpressed in different fibroproliferative diseases [47,48]. Our data suggest a role for the Hippo pathway in the negative regulation of ephrinB2/EphB2 receptor signaling in fibroblasts, limiting excessive fibroblast proliferation and inappropriate Schwann cell activation by fibroblasts.
Several studies have shown that EGF, by activating EGFR, leads to increased cell migration, a feature of tumour progression, metastasis and wound healing [49,50] but in other cell-types leads Figure 5. Willin is an upstream regulator of EphrinB2 and EGFR. (A) Willin expression is antagonistic to YAP EphrinB2 and EGFR transcriptional regulation. mRNA levels of Ephrin B2 and EGFR were probed by quantitative real-time PCR. mRNA levels were compared with the empty vector control fibroblast cells (set to 1). At the transcriptional level, Willin overexpression decreases EGFR and to a less extend EphrinB2 whereas YAP results in an increase in both EphrinB2 and EGFR mRNA levels. Means were calculated from Ct values in six independent experiments. bactin was used to normalize for variances in input cDNA. Error bars represent 6s.e. (n = 6). Fibro-vector vs fibro-Willin or fibro-YAP for the analysed genes: *p,0.05; **p,0.01; ***p,0.001; Student's t-test. Fibroblasts-vector vs fibroblasts-Willin: EphrinB2 (p = 0.055); Student's t-test. (B) Immunoblots analysis of EphrinB2 and EGFR show loss of expression upon ectopic Willin expression and gain of EphrinB2 expression upon YAP overexpression when compared to empty vector control (set to 1). b-actin was used as a loading control. Means were calculated from six independent experiments. Error bars represent 6s.e. (n = 6). Fibro-vector vs fibro-Willin or fibro-YAP: *p,0.05; **p,0.01; Student's t-test. Fibroblasts-vector vs fibroblasts-YAP: EGFR (p = 0.40); Student's t-test. (C) EGFR mRNA levels are upregulated upon Willin knockdown but EphrinB2 does not show statistical significant change at the transcriptional level. mRNA levels of Ephrin B2 and EGFR were probed by quantitative real-time PCR and mRNA levels compared with the fibro-siCtr cells (set to 1). Means were calculated from Ct values in six independent experiments. b-actin was used to normalize for variances in input cDNA. Error bars represent 6s.e. (n = 6). Fibro-siCtr vs fibro-siWillin cells: *p,0.05; Student's t-test. Fibro-siCtr vs Fibro-siWillin cells: EphrinB2 (p = 0.555); Student's t-test. (D) Immunoblots analysis of EphrinB2 and EGFR show upregulated expression when Willin is knockdown. Relative expression was determined compared to background expression (Fibro-siCtr cells set to 1). b-actin was used as a loading control. Means were calculated from three independent experiments. Error bars represent 6s.e. (n = 3). Fibro-siCtr vs Fibro-siWillin cells: **p,0.01; ***p,0.001; Student's ttest. doi:10.1371/journal.pone.0060028.g005 to increased cell proliferation [51,52]. High EGFR expression has been shown in wound repair of both epithelial and human skin fibroblasts by promoting cell migration and wound epithelialization [37,38,53]. In our experiments, an increase in ectopic Willin expression induced down-regulation of EGFR, with an increase in cell migration but inhibition/delay of cellular proliferation, whilst a decrease in Willin expression resulted in an increase in EGFR expression with a inhibition/delay of cell migration but an increase in cell proliferation. This suggests that Willin expression can influence the level of EGFR in sciatic nerve fibroblasts, and Figure 6. Willin antagonizes some of the genes regulated by YAP in sciatic nerve fibroblasts. mRNA expression of the YAP target genes CTGF, BMP2, FGF1, RASSF8, IGFBP3 and PRL was probed in fibroblasts expressing Willin or YAP by quantitative real-time PCR. mRNA levels were compared with the empty vector control (set to 1). Willin overexpression increased BMP2, FGF1, RASSF8, IGFBP3 and PRL mRNA levels and, together with YAP, decreased CTGF mRNA expression in these cells. Means were calculated from Ct values in three independent experiments. b-actin was used to normalize for variances in input cDNA. Error bars represent 6s.e. (n = 3). Fibroblasts-vector vs fibroblasts-Willin or fibroblasts-YAP for all the analysed genes: *p,0.05; **p,0.01; ***p,0.001; Student's t-test. Fibroblasts-vector vs fibroblasts-YAP: BMP2 (p = 0.217); Student's t-test. doi:10.1371/journal.pone.0060028.g006 that this in turn regulates the potential of over-proliferation, highlighting the fact that fibroblasts have different functions in a tissue-context dependent manner. In agreement with our observations, Merlin in mammalian cells also inhibits EGFR in mouse embryonic fibroblasts (MEFs) and cells undergo contactdependent inhibition of proliferation [54]. Furthermore, in Drosophila, Merlin and Expanded mutant cells show an upregulation of the EGFR signaling pathway [55]. It is also possible to hypothesise that there may also be an element of feedback control, as EGF itself has been shown to influence the cellular distribution of Willin [27]; and also, recently the EGFR ligand, amphiregulin, has been identified as a transcriptional YAP target [14].
Willin induced transcriptional activation of BMP2, FGF1, IGFBP3, PRL and RASSF8. Importantly, we observed that Willin and YAP displayed opposite effects on FGF1, IGFBP3, RASSF8 and PRL expression. Willin increased expression, while YAP decreased expression of these genes. While, BMP2 and RASSF8 displayed a similar trend in Willin-induced upregulation with the data presented by Moleirinho et al. (2013) and Angus et al. (2012) [9,11], in epithelial MCF10A cells, FGF1, IGFBP3 and PRL were regulated by Willin expression but displayed the opposite trend.
BMP2 has been shown to induce cell migration in different cell types, including MEF [56] and its involvement in the liver and peripheral nerve healing response has been previously described [57,58]. The pronounced Willin-induced upregulation of BMP2 could also explain the migratory pattern observed in the woundhealing assay. Expression of CTGF, a YAP direct target [39,59], in tissue wound repair has been proposed to be a major player in the pathogenesis of fibrotic processes [60]. We found it down regulated upon Willin expression.
Our data suggests that the hippo signaling pathway might have a significant role in both the development and maintenance of the mammalian peripheral nervous system. Specifically it would be of great interest to explore this further in both fibroblasts and Schwann cells to verify how the signaling cascade regulates the ephrin/Eph signaling, and its ability to control wound repair and, ultimately, regeneration of the PNS.