Pyocyanin Facilitates Extracellular DNA Binding to Pseudomonas aeruginosa Influencing Cell Surface Properties and Aggregation

Pyocyanin is an electrochemically active metabolite produced by the human pathogen Pseudomonas aeruginosa. It is a recognized virulence factor and is involved in a variety of significant biological activities including gene expression, maintaining fitness of bacterial cells and biofilm formation. It is also recognized as an electron shuttle for bacterial respiration and as an antibacterial and antifungal agent. eDNA has also been demonstrated to be a major component in establishing P. aeruginosa biofilms. In this study we discovered that production of pyocyanin influences the binding of eDNA to P. aeruginosa PA14 cells, mediated through intercalation of pyocyanin with eDNA. P. aeruginosa cell surface properties including cell size (hydrodynamic diameter), hydrophobicity and attractive surface energies were influenced by eDNA in the presence of pyocyanin, affecting physico-chemical interactions and promoting aggregation. A ΔphzA-G PA14 mutant, deficient in pyocynain production, could not bind with eDNA resulting in a reduction in hydrodynamic diameter, a decrease in hydrophobicity, repulsive physico-chemical interactions and reduction in aggregation in comparison to the wildtype strain. Removal of eDNA by DNase I treatment on the PA14 wildtype strain resulted in significant reduction in aggregation, cell surface hydrophobicity and size and an increase in repulsive physico-chemical interactions, similar to the level of the ΔphzA-G mutant. The cell surface properties of the ΔphzA-G mutant were not affected by DNase I treatment. Based on these findings we propose that pyocyanin intercalation with eDNA promotes cell-to-cell interactions in P. aeruginosa cells by influencing their cell surface properties and physico-chemical interactions.


Introduction
Planktonic bacterial cells tend to adhere, aggregate, colonize and grow into a mature biofilm on natural and synthetic surfaces. Biofilm formation is a serious ongoing problem ranging from lethal bacterial infections in humans, contamination of environmental resources such as water and corrosion of engineered systems [1][2][3]. Bacterial cells in biofilms are embedded in a matrix of extracellular polymeric substances (EPS) mainly composed of polysaccharides, proteins, lipids, metabolites and extracellular DNA (eDNA) [4,5]. Extracellular DNA is recognised as a pivotal biofilm component in various Gram negative and Gram positive bacterial species. For instance in the opportunistic human pathogen Pseudomonas aeruginosa eDNA is essential for biofilm formation, stability and protection against antibiotics [6,7]. In Streptococcus mutans, Staphylococcus epidermidis and Bacillus cereus, eDNA is essential for initial bacterial adhesion to surfaces. In Staphylococcus aureus eDNA enhances cellular aggregation and protects bacterial cells in biofilms against antibiotics and detergents [8][9][10][11][12].
In Gram positive bacteria quorum sensing (QS) dependent release of eDNA occurs through lysis of a small proportion of bacterial cells, mediated by proteins such as AtlE an autolysin produced by S. epidermidis [13], gelatinase (GelE) and serine protease (SprE) by Enterococcus faecalis [14]. In addition phage mediated and H 2 O 2 mediated eDNA release as a consequence of cell lysis have also been reported in various Streptococcus species [15,16]. However in Gram negative bacteria like P. aeruginosa, eDNA release is mediated through diverse mechanism. Quorum sensing involving N-acyl-L-homoserine lactones (AHL) and the Pseudomonas quinolone signal (PQS) activate eDNA release during late log phase in planktonic culture growth by inducing phage production [17]. QS independent mechanisms also cause cell lysis and subsequent eDNA release via flagella and type IV pili [17,18]. Interestingly, it was recently discovered that eDNA release in P. aeruginosa also occurs through oxidative stress caused by hydrogen peroxide (H 2 O 2 ) generation mediated by pyocyanin production [19].
Pyocyanin is a phenazine molecule produced abundantly by P. aeruginosa and is also controlled by QS [20]. The secondary QS molecule PQS controls the production of the phzA-G operons responsible for synthesis of the primary phenazine-1-carboxylic acid (PCA). PCA in P. aeruginosa PA14 strains is then modified to produce pyocyanin through action of the phzM gene product [21]. Pyocyanin controls gene expression and community behavior in divergent bacteria and colony size and biofilm thickness in P. aeruginosa PA14 [20,22,23]. Pyocyanin is electrochemically active and acts as an electron shuttle supporting P. aeruginosa cellular respiration and energy generation under oxygen deficient condition in biofilms, by accepting electrons for reoxidation of accumulating NADH [20]. Pyocyanin accepts electrons directly from NADH or NADPH and reduces molecular oxygen, forming reactive oxygen species like O 2 2 and H 2 O 2 . Reactive oxygen species change the redox balance of host cells causing cell injury and death [20] and hence pyocyanin is recognized as a virulence factor in chronic lung infections in cystic fibrosis patients [20].
Based on the facts that pyocyanin can act as an electron shuttle and eDNA promotes bacterial aggregation through acid-base interactions involving electron-donating and electron-accepting groups [9] we hypothesised that pyocyanin is involved in facilitating eDNA binding to P. aeruginosa cells. Further, we predicted that pyocyanin in association with eDNA could influence, P. aeruginosa cell surface properties such as size, hydrophobicity, surface energies and consequently influence cellto-cell interactions (aggregation).
We tested our hypothesis in aggregation assays using P. aeruginosa PA14 wildtype, DKN370 (pyocyanin overproducing) and a DphzA-G (phenazine/pyocyanin deficient) mutants before and after removal of eDNA and in the presence of exogenous DNA (only for wildtype and DphzA-G strains). The binding/ intercalation of pyocyanin with DNA was subsequently demonstrated directly. The surface properties of wildtype and DphzA-G strains before and after removal of eDNA were examined using techniques such as the Zetasizer to measure hydrodynamic diameter (size), goniometry for measuring hydrophobicity and thermodynamic analysis based on surface energies obtained from contact angles to reveal a possible mechanism of pyocyanin associated eDNA mediated aggregation in P. aeruginosa PA14.

P. aeruginosa Species and Culture Conditions
P. aeruginosa PA14 wildtype, DKN370 and DphzA-G (Note: The DKN370 and DphzA-G mutants was obtained from Dianne K. Newman) [22,23] strains used in this study were plated onto LB agar plates and incubated overnight under aerobic conditions at 37uC. Single colonies from the agar plates were used to inoculate 20 ml cultures in LB medium in 100 ml conical flasks at ph 7 for 1, 2 or 3 days at 30uC and 150 rpm. After growth, the P. aeruginosa PA14 strains were harvested and pelleted out by centrifugation at 50006g for 5 min at 10uC. Where indicated the pyocyanin deficient strain DphzA-G was also grown in the presence of 100 mm pyocyanin (Sigma-Aldrich) for 1, 2 and 3 days at 30uC, 150 rpm. To remove naturally present eDNA from the P. aeruginosa cell surfaces, bacterial suspensions were pre-treated with 40 units (2 units/ml) of DNase I (Life technologies) in the presence of 5 mM MgCl 2 for 90 min at room temperature under static conditions and subsequently washed twice with phosphate buffered saline (PBS) and resuspended in PBS.
Aggregation and Settling of P. aeruginosa PA14 before and after DNase I Treatment To study P. aeruginosa PA14 aggregation before and after DNase I treatment, bacterial cells were diluted to 2.5610 8 cells/ml in PBS. Optical density was determined in 1 ml plastic cuvettes (SARSTEDT) using a Bio-Rad Smartspec 3000 (Bio-Rad Laboratories, USA) at 600 nm. The bacteria were allowed to aggregate and settle for 60 min at room temperature in plastic cuvettes under static conditions while monitoring the decrease in optical density (OD) every 15 min, using a Bio-Rad Smartspec Figure 2. Influence of pyocyanin and DNase I treatment in aggregation of P. aeruginosa PA14. The % reduction in optical density after 60 min due to aggregation of PA14 strains before and after DNase I treatment. Error bars represents standard deviations from the mean (n = 3). Asterisks and hash indicate statistically significant (p,0.05) differences in % of aggregation in comparison to DNase I treated (DKN370 and wildtype) and DphzA-G strain (regardless of DNase I treatment) respectively. doi:10.1371/journal.pone.0058299.g002 Figure 3. Effect of DNA addition in aggregation of P. aeruginosa PA14. The % reduction in optical density after 60 min due to aggregation of PA14 wildtype and DphzA-G strains before and after addition of 1 mg/ml of final concentration of exogenous DNA isolated from P. aeruginosa cultures. Error bars represents standard deviations from the mean (n = 3). Asterisks indicate statistically significant (p,0.05) differences in % of aggregation in comparison to DNase I treated wildtype strain. doi:10.1371/journal.pone.0058299.g003 3000. Settling as a consequence of aggregation was quantified as a percentage reduction in OD after 60 min [24] OD 0 min {OD 60 min OD 0 min |100 ð1Þ where OD 0min is the initial OD at the start of an experiment and OD 60min is the OD after 60 min. All measurements were done at 600 nm using PBS without bacteria as blank.
Chromosomal DNA Isolation from P. aeruginosa PA14 Chromosomal DNA was extracted from a one day old culture of P. aeruginosa PA14 using standard protocols [9]. The extracted DNA was then dissolved in MilliQ water and the DNA concentration was determined using a Nanodrop UV/Vis spectrophotometer. Purity of the DNA was determined by the absorbance ratio A 260 /A 280 (DNA/protein) . All DNA samples used in this study have A 260 /A 280 equal to 1.8, indicating a high purity.
Aggregation and Settling of P. aeruginosa PA14 in the Presence of Exogenous DNA A total of 2.25610 8 cells in 900 ml PBS were mixed with 100 ml of a DNA solution or 100 ml MilliQ water as a negative control in 1 ml plastic cuvettes by pipetting. The final concentration of DNA present was 1 mg/ml. The settling of bacterial cells due to aggregation mediated by the presence or absence of exogenous DNA was monitored every 15 min.

Hydrodynamic Diameter of P. aeruginosa PA14
The hydrodynamic diameter of P. aeruginosa PA14 wildtype and DphzA-G strains of cell density 2.5610 8 cells/ml before and after DNase I treatment was measured in PBS using a Zetasizer (nano series Model Number ZEN3600) (Malvern Instruments Ltd) at 25uC. Backscattered light from a monochromatic laser (633 nm wavelength) after passing through the bacterial cell suspension was detected at an angle of 173u. Subsequently, the autocorrelation of the time-dependent intensity fluctuations of the scattered light were used to derive the Brownian motion velocity of the bacteria, from which the hydrodynamic diameter was then calculated using the Stokes-Einstein equation [25].

Pyocyanin-DNA Binding
Binding of pyocyanin to DNA was demonstrated using a standard ethidium bromide-DNA binding technique [26] using a fluorometer (Varian Cary Eclipse Fluorescence Spectrophotometer by Varian Australia Pvt. Ltd). DNA (4 ng/ml) was mixed with ethidium bromide (4 mM) and various concentrations of pyocyanin in SHE buffer (2 mM Hepes, 10 mM EDTA and 9.4 mM NaCl in MilliQ water adjusted to pH 7 with NaOH). Light emission at 615 nm (l ex = 480 nm) was quantified at room temperature.
To further visualize pyocyanin-DNA binding, DNA (2 mg) isolated from P. aeruginosa PA14 wildtype was mixed with pyocyanin at 500 mM (Sigma-Aldrich) at both acidic (4.5) and neutral pH by pipetting. At acidic pH pyocyanin appears pink, while at neutral or alkaline pH it appears blue. The DNApyocyanin solution was incubated static overnight at room temperature followed by centrifugation at 50006g for 5 min at 10uC in order to pellet out the DNA from the pyocyanin solution. The supernatant was removed by pipetting and the DNA pellet was air-dried. The colour of DNA pellets was recorded using using a digital camera.

Contact Angles Measurements and Surface Thermodynamical Analysis of P. aeruginosa PA14
For contact angle measurements, bacterial lawns were prepared by depositing bacteria from suspension on a 0.2 mm-pore-diameter filter (Nitrocellulose membrane filter, Millipore) using negative pressure [8]. The filters were air dried at room temperature until contact angles of sessile water droplets were stable (30-45 min). Contact angles were measured with standard polar and non-polar liquids i.e. water, formamide and diiodomethane respectively. Where indicated a bacterial lawn of DNase I treated P. aeruginosa was prepared and its contact angle was measured as above.
To conduct surface thermodynamical analysis, the measured contact angles of P. aeruginosa cell surface before and after DNase I treatment were converted into its Lifshitz-Van der Waals (c LW ) and acid-base (c AB ) surface free energy components using the LW-AB approach [27,28]. Subsequently, the acid-base component was separated in an electron-donating (c 2 ) and electron-accepting (c + ) parameter [27]. Different components of the surface energies were then further used to calculate the total interfacial free energy of aggregation (Total DG) at close contact as separated in a Lifshitz-Van der Waals (LW DG) and an acid-base (LW DG) part [28].

Statistical Analysis using Student t-test
The effects of naturally produced pyocyanin and DNase I treatment on P. aeruginosa PA14 aggregation, contact angle and surface thermodynamics were analyzed using a two-tailed Student's t-test. A similar analysis was carried out on the effect of exogenous DNA addition on P. aeruginosa aggregation. Differences were considered significant if p,0.05.

Results
Pyocyanin Production and Effect of DNase I Treatment in Settling of P. aeruginosa Strains In this study, P. aeruginosa PA14 cell surface properties and aggregation were investigated in the presence and absence of eDNA. The DKN370 and wildtype strain producing pyocyanin was compared with the pyocyanin deficient mutant strain DphzA-G. Figure 1A illustrates the visible difference between the three strains with respect to pyocyanin production. Figure 1B demonstrates the settling behaviour of the DKN370, wildtype and DphzA-G culture before and after DNase I treatment, due to aggregation, indicated by decreases in optical density at 600 nm over time. Quantification of Settling of DKN370, Wildtype and DphzA-G mutant of P. aeruginosa before and after DNase I Treatment Figure 2 shows that P. aeruginosa PA14 strains showed maximum settling up to 39%, 37% and 22% for the DKN370, wildtype and DphzA-G mutant respectively when grown over one day and before DNase I treatment. As the growth period increased the percentage of settled cells decreased drastically down to 12, 10 and 3% for DKN370, wildtype and DphzA-G respectively over 3 days of growth. Before DNase I treatment, settling of P. aeruginosa PA14 DKN370 and wildtype was always significantly higher in comparison to its pyocyanin deficient counterpart regardless of growth days. After DNase I treatment the settling percentage of the DKN370 and wildtype decreased significantly to a level equivalent to the DphzA-G mutant percentage. However, no difference in the DphzA-G settling percentage was observed when treated with DNase I. Interestingly when DphzA-G was grown with 100 mm pyocyanin a reduction in the settling percentage was observed after DNase I treatment although the difference was significant only after 3 days.
Effect of DNA Addition in Settling of P. aeruginosa Figure 3 shows that exogenous addition of 1 mg/ml DNA (isolated from P. aeruginosa cultures) to 2.25610 8 DNase I treated P. aeruginosa PA14 cells resulted in significant increases in the settling percentage of the wildtype strain only. The phenazine deficient mutant DphzA-G did not show any increase in percentage settling when treated with exogenous DNA. When the DphzA-G mutant was grown in the presence of exogenously added pyocyanin only a slight variation in settling percentage was observed, indicating endogenous production of eDNA, pyocyanin or both was required.
Hydrodynamic Diameter (size) Analysis of P. aeruginosa Wildtype and DphzA-G Strains The hydrodynamic diameter of P. aeruginosa PA14 wildtype before DNase I treatment showed a gradual increase in size from 10656170 to 14406300 nm with the increase in growth period from 1 to 3 days respectively. Whereas, the DphzA-G mutant showed a smaller variation in size (9206175 to 10506160 nm) and remained in the same size range after DNase I treatment. In contrast, the wildtype strain showed a steady decrease in its size after DNase I treatment, ultimately close to the size of the DphzA-G mutant strain (Figure 4A-C). Figure 4D demonstrates clearly that after DNase I treatment the hydrodynamic diameter of the wildtype reduced to about 180688 nm after 1 day growth to a maximum of 3826197 nm after 3 days of growth. In parallel, the mutant DphzA-G showed negligible reduction in its hydrodynamic diameter after DNase I treatment ranging from 215 to 70 nm only. Figure 5A shows the cell pellet formed after centrifugation of a 3 day old P. aeruginosa PA14 culture. The wildtype forms a very small pellet encased in EPS. In contrast the DphzA-G forms a larger pellet lacking EPS. The EPS in the wildtype pellet was removed when subjected to DNase I treatment whilst no difference was observed in the case of DphzA-G mutant after DNase I treatment (Fig. 5). Figure 6A shows the relative fluorescence emission of ethidium bromide before and after addition of DNA. The initial intensity of the ethidium bromide emission peak which was only 3.2 rose gradually after addition of DNA as a function of concentration (data not shown) and reached 15.2 with 4 ng/ml DNA. The addition of 40-140 mM pyocyanin reduced the intensity of the pellet from white to greenish blue in neutral pH and pink under acidic pH, after treated with pyocyanin and incubated at room temperature for overnight, indicates pyocyanin binded with DNA and the reappearance of white colour of DNA by simple washing of coloured DNA pellet with water resulted in removal of pyocyanin stain (Fig. 6D and E). doi:10.1371/journal.pone.0058299.g006 Table 1. Contact angles of P. aeruginosa PA14 strains before and after DNase I treatment using water, formamide and diiodomethane and their surface free energy components: Lifshitz-Van der Waals (c LW ), acid-base (c AB ), electron-donating (c 2 ) and electron-accepting (c + ) based on their contact angle values. fluorescence output indicative of displacement of ethidium bromide from DNA. Pyocyanin binding with DNA was further confirmed by mixing DNA with synthetic pyocyanin and incubating overnight at room temperature. A change in the colour of DNA pellets from white to greenish blue in neutral pH and pink under acidic pH was observed. Washing the coloured DNA pellets with water removed the colour indicating that the binding of pyocyanin with DNA is reversible (Fig. 6D and E).

Binding of Pyocyanin with DNA
Contact Angle and Subsequent Surface Energy Analysis of P. aeruginosa Strains before and after DNase I Treatment Table 1 illustrates the changes in surface hydrophobicity and surface energy of one day old cultures of P. aeruginosa PA14 wildtype and mutant before and after DNase I treatment. Untreated PA14 wildtype showed a higher degree of water contact angle in comparison to untreated DphzA-G. After DNase I treatment only the wildtype strain showed a significant decrease in water contact angle and ultimately equivalent to the water contact angle of the DphzA-G mutant. The surface energy components: Lifshitz-Van der Waals remained unaffected between wildtype and DphzA-G regardless of DNase I treatment. However, the electron donating and electron accepting parameters of the acid-base component was significantly different between the wildtype and mutant strains in the absence of DNase I treatment.

Effect of Pyocyanin Production and DNase I Treatment on Interfacial Free Energy of Aggregation of P. aeruginosa Strains
Interfacial aggregation energies at close approach are presented in Figure 7. Lifshitz-Van der Waals aggregation energies were attractive for both strains, regardless of DNase I treatment ( Figure 6A). The values for acid-base aggregation energies were very low 3 mJ/m 2 for the wildtype strain before DNase I treatment and drastically high (40 mJ/m 2 ) after DNase I treatment. In contrast, the acid-base energies of DphzA-G strain remained unchanged (45 mJ/m 2 ) even after DNase I treatment (Fig. 7B). Similarly, the value for total aggregation energies is almost zero for the wildtype strain before DNase I treatment. After DNase I treatment the total aggregation energies for wildtype increased drastically, whereas the DphzA-G remained highly positive between 40 to 45 mJ/m 2 regardless of DNase I treatment (Fig. 7C).

Discussion
Pyocyanin and eDNA are both currently recognized as pivotal components in the development of P. aeruginosa biofilm [6,20,22]. Whilst the aggregation of bacteria is dependent of a variety of factors there is clear evidence that attachment of eDNA to cell surfaces influences physico-chemical interactions and facilitates aggregation [9]. There is also clear evidence that pyocyanin plays a role in eDNA release [19] and respiration under electron acceptor limited conditions [20]. Here we show for the first time that naturally produced pyocyanin promotes eDNA binding to P. aeruginosa cells and consequently influences cell surface properties, physico-chemical interaction between cells and aggregation.
In the presence of eDNA acid-base and total interaction energies represent an extremely low energy barrier that microbial cells can overcome through hydrogen bonding due to the presence of biopolymers and cell appendages that extend hundreds of nanometers from the cell surface [29]. In support of this, the removal of eDNA by DNase I treatment in this study, resulted in a drastic increase in the acid-base and total energy barrier to aggregation up to 14 and 70 fold respectively. Consequently the acid-base and total interaction energies become highly repulsive and bacterial aggregation decreased significantly ( Fig. 2 and 7).
Interestingly, DNase I treatment did not have any effect on aggregation of a pyocyanin deficient mutant, which has a reduced ability to aggregate compared with the pyocyanin overproducing DKN370 and normal amount of pyocyanin producing wildtype (Fig. 2). It should be noted that DKN370 also showed a similar level of aggregation to its wildtype strain despite over production of pyocyanin (Fig. 2). It has been recently discovered that pyocyanin over production can enhance the release of eDNA [19] however binding of eDNA to bacterial cells is a limiting factor, once the bacterial cells surfaces are saturated with eDNA, further addition of DNA does not encourage further increase in adhesion or aggregation [9,30]. Further, the acid-base and total interactions energies of pyocyanin deficient mutant remained unchanged and highly repulsive regardless of DNase I treatment (Fig. 7). However, DNase I treatment did affect aggregation of a mutant strain grown in the presence of exogenously added pyocyanin although the results are significant only on day 3 of culturing (Fig. 2). It could be due to the higher swarming motility of phenazine deficient mutants that makes them not responsive to exogenous pyocyanin at early stages of biofilm development [22]. However during later stages of biofilm growth they become responsive to pyocyanin and promote biofilm development [22].
In connection with the current study phenazine deficient mutants may possibly become responsive to exogenous pyocyanin only at 3 day culture and thus binds with eDNA and enhanced aggregation (Fig. 2). Additionally, when DNase I treated wildtype and DphzA-G mutant were exposed to exogenously added DNA the wildtype strain showed a significant increase in aggregation whilst the pyocyanin deficient mutant did not (Fig. 3). Taken together, these results are consistent with the hypothesis that pyocyanin facilitates cell aggregation through an interaction with eDNA. A fluorescence assay, involving displacement of the DNA intercalating agent ethidium bromide from DNA, was used to confirm that pyocyanin binds directly to DNA. DNA intercalation occurs via noncovalent interactions and is reversible. In this study pyocyanin binding was reversible as shown by the decolourization of pyocyanin-DNA pellets after washing with water. DNA intercalating agents cause substantial changes in DNA structure including alteration of the hydrodynamic properties of DNA [26]. It is likely such changes contribute to the enhancement of eDNA cross-linking with biopolymers and hence increases cellular aggregation.
Supporting the above observation and argument, the hydrodynamic diameter of wildtype and DphzA-G P. aeruginosa PA14 cells showed clear variation with the size of the wildtype decreasing to the size of the pyocyanin deficient mutant after DNase I treatment (Fig. 4). This result is consistent with the suggestion that abundant biopolymers in EPS such as eDNA are anchored to the bacterial cell surface with the assistance of pyocyanin influencing surface properties such as contact angle and surface energy ( Table 1). These surface properties are a reflection of the chemical composition of bacterial cell surfaces and this chemical composition drives attractive or repulsive physico-chemical interactions (Fig. 6). Without the interaction between DNA and pyocyanin the EPS layer loses stability and can be removed from bacterial cell pellets as shown in Figure 5. Figure 8 illustrates for clarity how it is envisaged that DNase I treatment affects cell surface properties of a pyocyanin producing cell in comparison to a pyocyanin deficient cell.
The competence of bacteria to bind and uptake eDNA from the extracellular environment has been well studied on various Streptococcus strains. In S. intermedius and S. mutans the development of competence (i.e. uptake of DNA from the environment) is mediated by a QS molecule called competence stimulating peptide (CSP) [30]. CSP triggers DNA binding and uptake, and simultaneously induces the synthesis of a DNA binding protein ComGB [31,32] and henceforth increases bacterial aggregation and biofilm formation. Based on the data presented pyocyanin appears to play this critical role in facilitating eDNA binding to the P. aeruginosa cell surface and influencing its cell surface properties and aggregation.
Previous studies suggest that the production of phenazines enhances electron shuttling, antibacterial property, responsible for virulence factor in chronic lung infections, enhance eDNA production, microcolony formation and increased biomass in biofilms and eDNA is essential for biofilm stability and development of P. aeruginosa [6,19,22,33]. We conclude that pyocyanin facilitates eDNA binding in P. aeruginosa and thus keeps the EPS integrated and attached to bacterial cell surfaces and consequently promotes bacterial aggregation, biofilm development and stability. Further, we propose that the thick and integrated EPS that forms due to eDNA production in association with pyocyanin is possibly shielding the bacterium in biofilms against physical and chemical challenges include shear stress, antibiotics and detergents. Production of pyocyanin and pyocyanin mediated eDNA shielding could give P. aeruginosa a competitive edge by protecting it from its competitor bacterial species that endure in mixed biofilms, thereby having an ecological influence in mixed biofilms. Further existence of pyocyanin along with eDNA in the sputum of cystic fibrosis (CF) patients [20,34] could not only encourage biofilm formation but also enhance mortality in CF patients through deterioration of the host immune system. We believe this discovery may result in new strategies to combat biofilm based infections.