A Natural Small Molecule Harmine Inhibits Angiogenesis and Suppresses Tumour Growth through Activation of p53 in Endothelial Cells

Activation of p53 effectively inhibits tumor angiogenesis that is necessary for tumor growth and metastasis. Reactivation of the p53 by small molecules has emerged as a promising new strategy for cancer therapy. Several classes of small-molecules that activate the p53 pathway have been discovered using various approaches. Here, we identified harmine (β-carboline alkaloid) as a novel activator of p53 signaling involved in inhibition of angiogenesis and tumor growth. Harmine induced p53 phosphorylation and disrupted the p53-MDM2 interaction. Harmine also prevented p53 degradation in the presence of cycloheximide and activated nuclear accumulation of p53 followed by increasing its transcriptional activity in endothelial cells. Moreover, harmine not only induced endothelial cell cycle arrest and apoptosis, but also suppressed endothelial cell migration and tube formation as well as induction of neovascularity in a mouse corneal micropocket assay. Finally, harmine inhibited tumor growth by reducing tumor angiogenesis, as demonstrated by a xenograft tumor model. Our results suggested a novel mechanism and bioactivity of harmine, which inhibited tumor growth by activating the p53 signaling pathway and blocking angiogenesis in endothelial cells.


Introduction
The tumor suppressor p53 plays a key role in the regulation of cell cycle, apoptosis, DNA repair, and senescence [1] in response to diverse stress stimuli, including DNA damage, oncogene activation and hypoxia [2]. p53 acts as a transcriptional factor and activates various genes to exert specific functions involved in tumor development. Murine double minute 2 (MDM2), the main regulator of p53, inhibits the function of p53 through direct interaction [3].
In addition to the direct effect of targeting tumor cells, there are accumulating evidences that show activation of p53 may also effectively inhibit angiogenesis, which is one of the most important hallmarks in the cancer development [4], and is critical for tumor growth and metastasis [5]. Overexpression of p53 inhibits angiogenesis by up-regulation of its downstream target genes such as thrombospondin 1 (TSP-1) [6] and brain-specific angiogenesis inhibitor 1 (Bai1) [7]. Therefore, the p53 activation is also fundamentally involved in angiogenic processes [5].
Because of the high potential of p53 to elicit apoptosis or growth arrest in cells, pharmacological reactivation of the p53 tumor suppressor is a promising strategy for anti-cancer therapy [8]. Recently, proposed model suggested that p53 activation in vivo includes three major steps: (1) p53 stabilization, (2) release from MDM2 (i.e. antirepression) [9], and (3) promoter-specific activation [10]. Previous reports showed that some small compounds induces cancer cell cycle arrest and apoptosis through restoration of p53 pathway [2,11] and some other small molecules such as Inauhzin were identified that induced the level and activity of p53 consequently and effectively repressed the growth of xenograft tumours [12]. Indeed, several p53-reactivating compounds are currently being tested in clinical trials, including mutant p53-reactivating PRIMA-1 analog APR-246 [8].
Harmine, a small-molecule b-carboline alkaloid, is a naturally occurring compound in some plant species [13]. Previous research has shown that harmine plays some roles in anti-cancer treatments [14], as well as possesses anti-leishmanial properties [15] and an anti-viral effect [16] via multiple signaling pathways such as kinase [17] and mitochondrial signaling pathways [18]. However, there are no evidences that show harmine or its analogues exert their bioactivities via the p53 signaling pathway.
In the present study, we identified harmine as a novel activator of the p53 pathway and characterized its anti-angiogenic and antitumor effects via p53 signaling pathway in endothelial cells.

Cell Lines, Animals and Regents
Human umbilical vein endothelial cells (HUVECs) were purchased from Sciencell Research Laboratories (Beijing, China). Human A549 lung cancer cells provided by ATCC were cultured in RPMI 1640 medium. Mice were obtained from National Rodent Laboratory Animal Resources (Shanghai, China). All animal procedures were approved by the institutional Animal Ethics Committee of East China Normal University. Vascular endothelial growth factor (VEGF) and growth factor-reduced Matrigel were purchased from R&D Biosciences (San Diego, CA). p53-siRNA and negative control oligonucleotides were purchased from GenePharma (Shanghai, China). Harmine ($98% purity) was obtained from Sigma-Aldrich).

ProteOn XPR36 Protein Interaction Array
Measurement of binding affinities between harmine and MDM2 was performed as described elsewhere [19] with some modification. Briefly, a GLH chip was used in this assay. glutathione S-transferase (GST) and GST-MDM2 were dissolved in 10 mM NaAc and immobilized onto separate erect channels of the sensor chip by general amine coupling. MDM2-GST was immobilized to around 17400 RUs. After baselines were stable, harmine was dissolved in PBS-T buffer flowing through the chip horizontally. Data were analyzed with ProteOn manager TM software using the Langmuir model (A+B « AB) for kinetic data fitting.

Immunofluorescence Staining
As described in a previous report [20], Harmine-treated HUVECs were fixed using 4% formaldehyde and washed with phosphate buffered saline (PBS). Fixed cells were treated with 0.2% Triton-X 100. After blocking in 0.5% bovine serum albumin, cells were incubated with the primary antibody at room temperature, followed by incubation with the secondary antibody. Then, 49,6-diamidino-2-phenylindole (DAPI) was added to samples, followed by incubation while protected from light. Images were recorded by confocal microscopy (Leica).

Cell cycle, Apoptosis and Proliferation Analyses
For cell cycle analysis, cells were harvested and washed with PBS. Then, collected cells were re-suspended in 70% cold ethanol and fixed overnight at 4uC. After centrifugation, cells were resuspended with PBS containing RNase and incubated at 37uC for 30 minutes. Cells were then stained with PI and analyzed by flow cytometry. Hamine-induced apoptosis of HUVECs or HUVECs transfected with p53 siRNA was determined was quantified by an annexin V-FITC/PI dual staining assay. Briefly, cells were collected, centrifuged and re-suspended with PBS. After centrifugation, cells were re-suspended with binding buffer and incubated with annexin V and PI. Analysis was performed by flow cytometry. The effect of harmine on the proliferation of HUVECs was assessed by an MTS assay according to methods described elsewhere [21]. HUVECs or human A549 lung cancer cells were seeded in 96-well plates (5000 cells/well). After culture for 12 hours, cells were treated with various concentrations of harmine for 48 hours. Then, an AQueous One solution (Promega, Madison, WI) was added, and absorbances were measured with a microplate reader (SpectraMax 190; Molecular Devices).

P53 Silencing Using p53 siRNA
HUVECs were seeded in 6-well plate and transfected with p53 siRNA the next day. The effect of siRNA for p53 silencing was examined by Real-Time RT-PCR and western blot after the transfection for 36 hours and 72 hours respectively. After transfection for 16 hours, cells were treated with various concentrations of harmine for 48 hours.

Comet Assay
Alkaline comet assays were performed according to the previous study [22] with some modifications. In brief, HUVECs were treated with various concentrations of harmine for 48 hours, and then harvested. About 5 6 10 4 cells treated with harmine or untreated in 40 ml of phosphate-buffered saline mixed with an equal amount (40 ml) of 1% lower melting agarose were pipetted onto the slides that were covered with 100 ml of 0.5% normal molten agarose at 60uC. The cell suspension spread using a coverslip, and the slides were maintained at 4uC for 15 min to allow the gel to solidify. After removal of the coverslip, the slides were immersed in freshly prepared cold lysis solution (2.5 M NaCl, 100 mM Na2EDTA, 10 mM Tris, pH 10.0, 1% sodium sarcosinate) with 1% Triton X-100 at 4uC for 1 hour. Electrophoresis was carried out in a horizontal gel electrophoresis tank filled with fresh electrophoresis solution (1 mM Na2EDTA, 300 mM NaOH, pH 13.0) for 20 min (300 mA at 10 V) 4uC. Slides were rinsed twice in Tris buffer (0.4 M Tris, pH 7.5) for 15 min (neutralizing the excess alkali) and stained with 75ml of propidium iodide (5 mg/ ml) for 30 min. The slides were then examined using a Leica fluorescence microscope under 6 200 magnification.

Cell Migration and Tube Formation Assays
HUVECs were cultured in 6 well plates precoated with 1% gelatin (Sigma-Aldrich) and grown to confluency. After the treatment of mitomycin C for 2 hours, the cells were scratched with a pipette tip followed by the addition of harmine. Images of wound area were recorded after 8 hours, and the number of migrated cells was counted. Another migration test of HUVECs was assessed by a modified transwell assay as described elsewhere [23]. As for 549 lung cancer cells migration assay, cells were seeded in 6 well plates and grown to confluency. After the starvation overnight, the following steps were described as above. After the treatment for 12 hours, the number of migrated cells was counted. During the migration assays, we did not find the phenomenon of apoptosis. For the tube formation assay, 2610 4 cells per well were seeded in pre-cooled 96 well plates coated with Matrigel [24] and containing medium with or without harmine, followed by incubation at 37uC with 5% CO 2 . After 12 hours, tube structures in eight randomly chosen fields were photographed and counted by scoring the enclosed networks.

Ex vivo and In vivo Angiogenesis Assays
A rat aortic ring assay was performed as described elsewhere [25] with some modification. Briefly, thoracic aortas were sliced into rings of 1-1.5 mm in circumference. VEGF (200 ng) with or without harmine in serum-free MCDB131 medium was added to aortic rings coated with Matrigel. Medium was exchanged every 2 days. On day 7, microvessel outgrowths were photographed and analyzed. A mouse corneal micropocket assay was performed as described elsewhere [26] with some modification. Briefly, slowrelease pellets (0.35 6 0.35 mm) containing 200 ng VEGF with or without 5 mg harmine were prepared with a sucrose octasulfatealuminum complex and poly-HEMA. A corneal micropocket was created in the eye of a 4-5-week-old C57BL/6 mouse with a modified needle, and pellets were implanted into these micropockets. Chlortetracycline hydrochloride ophthalmic ointment was applied to each operated eye to prevent infection. After 7 days, the vessel length and clock hours of new blood vessels were examined under a stereomicroscope and recorded. The area of neovasculature was calculated according to the formula: Area (mm 2 ) = 0.2 6 p 6 VL (mm) 6 CN (mm), where VL is the maximal vessel length extended from the limbal vasculature toward the pellet and CN is the clock hours of neovascularization, 1 clock hour equals 30 degrees of arc.

Tumor Xenograft Model and Immunohistochemistry
Five-week-old nude mice were allowed to acclimate for a week before inoculation with A549 cancer cells. Cells (2 610 6 ) prepared in PBS were injected subcutaneously. After the tumor grew to 50-100 mm 3 , mice were intralesionally injected with harmine (30 mg/kg) every day for 21 days. An equivalent volume of DMSO was injected as a control. Measurements of body weight and tumor dimensions were performed daily. The tumor volume was considered the volume equal to 0.52 6 AB 2 , where A is the long axis and B is the short axis.
As described elsewhere [27], solid tumors were removed from sacrificed mice and fixed with 10% formaldehyde. Paraffinembedded tissues were sectioned at 4 mm and then stained using a Blood Vessel Staining Kit (Millipore). The optical density of stained blood vessels was analyzed by Image-Pro Plus software, according to the following formula: Mean integrated optical density (IOD) = IOD/area of the tumor section.

Statistical Analysis
Results were statistically analyzed using the Students t-test with Microsoft Excel. Some statistical analyses were performed using two-way analysis of variance followed by the Bonferroni post-hoc test using the GraphPad Quickcalc online web site. All experiments were repeated at least three times. A value of P,0.05 was considered significant.

Results
Harmine Activates p53 by Inducing Phosphorylation of p53 and Binding to MDM2 A number of reports show that inhibiting the MDM2-p53 interaction can be potentially important for activation of p53 and cancer therapy [3]. We first tested the effect of harmine on the p53-MDM2 interaction in HUVECs using a co-immunoprecipitation assay with anti-p53 and anti-MDM2 antibodies. Our data showed that the amount of MDM2 associated with p53 was dramatically decreased with increasing concentrations of harmine, although endogenous p53 was increased with the addition of harmine ( Figure 1B), suggesting that harmine inhibited the endogenous interaction of p53 with MDM2 in a dose-dependent manner.
In the p53 signaling pathway, modifications of p53 phosphorylation at various sites, such as serines 15, 20, and 37, are involved in regulation of p53 functions. p53 phosphorylation deters the association between p53 and MDM2 and prevents p53 ubiquitinization that degrades p53 [28]. To understand the effect of harmine on p53 phosphorylation, we examined three serines sites of p53 by western blot. As shown in Figure 1C and Figure 1D, harmine significantly induced p53 phosphorylation at ser-37 and ser-15 sites in a dose-dependent manner, but had little effect on p53 phosphorylation at ser-20 in endothelial cells. Phosphorylation of p53 occurs in response to some stimulies such as DNA damage. To test the phosphorylation of p53 induced by harmine due to DNA damage, we used cH2AX as a marker for DNA damage and performed comet assay. As shown in Figure S1, harmine did not induce DNA damage obviously. These data suggested that harmine induced p53 phosphorylation maybe not because of DNA damage.
On the other hand, The p53-MDM2 interaction is a pivotal event involved in tumor progression [29], and targeting the MDM2-p53 interaction by small molecules to reactivate p53 has emerged as a promising new strategy for cancer therapy [30]. To determine whether harmine bound to MDM2, we examined the interaction of harmine with MDM2 using a ProteOn XPR36 Protein Interaction Array. Quantitative analysis of the binding data under the deduction of GST showed that harmine rapidly bound to MDM2 protein with a K D of 48 mM in an association phase of 70 seconds ( Figure 1E and Table 1). Binding of harmine to MDM2 was characterized by a very fast association and dissociation ( Figure 1E). This result was fitted by the simplest 1:1 interaction model (Langmuir model).

Harmine Induces p53 Stabilization and Nuclear Accumulation
As a negatively regulator of p53, MDM2 promotes p53 degradation [31]. On the other hand, interaction with MDM2 protein also prevents p53 from degradation and promotes p53 stabilization [32]. Therefore, we examined whether expression levels of p53 and MDM2 were affected in harmine-treated cells.
Results showed that harmine upregulated p53 protein expression in a dose-dependent manner, but had little effect on the protein expression of MDM2 ( Fig. 2A). Next, we examined the stabilization of p53 induced by harmine. As shown in Figure 2B, in the absence of harmine and in the presence of cycloheximide (CHX), which inhibits protein synthesis, the protein level of p53 was lower in cells treated with CHX for 8 hours, compared with that in untreated endothelial cells, indicating p53 degradation during inhibition of protein synthesis. Conversely, in the presence of CHX, the protein level of p53 did not significantly change in harmine-treated endothelial cells. These results suggested that harmine prevented p53 degradation and induced stabilization of p53 in endothelial cells.
A previous report showed that interruption of the p53-MDM2 interaction and p53 phosphorylation results in nuclear accumulation of p53 [33]. To investigate nuclear accumulation of p53 induced by harmine, we examined the expression level of p53 in the nucleus and cytoplasm by western blot using an anti-p53 antibody. As shown in Figure 2C, nuclear levels of p53 were elevated significantly in cells exposed to harmine (left panel), while cytoplasmic levels of p53 hardly changed with the addition of harmine (right panel). To confirm nuclear accumulation of p53 induced by harmine, we examined the expression level of p53 in the nucleus by immunofluorescence staining using the anti-p53 antibody. Our data indicated that harmine increased nuclear localization of p53 in a dose-dependent manner ( Figure 2D). These results suggested that p53 stabilization resulted from inhibition of the p53-MDM2 interaction, and p53 phosphorylation induced by harmine was an important factor for nuclear accumulation in endothelial cells.

Harmine Induces p53 Transcriptional Activity
When the p53-MDM2 interaction is disrupted and the protein level of p53 in the nucleus is increased, transcriptional activity of p53 is activated. To further investigate the effect of harmine on the p53-MDM2 signaling pathway, we examined the expression levels of p53-target genes such as p21, survivin, CDC2, cyclin B1 and CDK2. As shown in Figure 3A, left panel, harmine increased the mRNA level of p21, and inhibited the expression of survivin, CDC2, cyclin B1 and CDK2 in a dose-dependent manner, suggesting that harmine increased p53 nuclear accumulation and activated its transcriptional activity in endothelial cells. Accordingly, harmine inhibited the expression levels of both cyclin B1 and CDC2, which are critical regulators of the G2/M checkpoint [34]. Furthermore, harmine inhibited the expression levels of cyclin A and CDK2, but did not affect the expression of cyclin E and cyclin D1.
To understand the molecular mechanism of harmine in endothelial cells, which are very important in angiogenesis, we examined the expression of downstream p53-target genes, TSP-1 [6] and brain angiogenesis inhibitor 1 (Bai1) [7], which are both endogenous angiogenesis inhibitors. As shown in Figure 3B

Harmine Induces Apoptosis and Cell Cycle Arrest in HUVECs
As a cellular gatekeeper, activation of p53 induces cell growth arrest and apoptosis [35]. To identify the function of harmine in apoptosis, we performed a cell apoptosis assay using annexin V/PI staining. As shown in Figure 4A, left panel, 20 mM harmine increased HUVEC apoptosis (15.45%) by 3-fold compared with that in untreated cells (5.34%). Harmine-induced HUVEC apoptosis was further confirmed by cleavage of poly (ADP-ribose) polymerase (PARP), a marker of apoptosis ( Figure 4A, right panel) [36], and by the decrease of anti-apoptotic proteins Bcl-2 [37] and survivin [38] in endothelial cells ( Figure 4A, right panel).
To further identify whether harmine induced apoptosis in p53depemdent manner, we tried to silence the p53 gene with siRNA targeting p53 mRNA in HUVECs. As shown in Figure S2, 20 mM harmine did not significantly increased HUVEC apoptosis (13.42%) compared with that in untreated cells (11.08%). These results suggested that harmine-induced apoptosis may depend on p53 pathway in HUVECs.
We further examined the effect of harmine on the cell cycle progression of endothelial cells. In the presence of harmine, cells were visibly arrested in G2/M, accompanied by a increased G2/ M phase of the cell cycle ( Figure 4B and Table 2). To evaluate the effect of harmine on HUVEC proliferation, we performed a cell proliferation assay. Harmine treatment of HUVECs resulted in inhibition of HUVEC proliferation as indicated by a decrease of absorbance at 490 nm, comparing with that of the control ( Figure 4C).

Harmine Inhibits HUVEC Migration and Tube Formation
To evaluate the effect of harmine on the migration of HUVECs, we performed wound healing and transwell migration assays. Quantitative analysis by counting the number of migrated cells showed that harmine inhibited cell migration in a dose-dependent manner ( Figure 5A and 5B). Endothelial cells are capable of forming capillary-like structures in vitro, which mimics the process of capillary formation in vivo. Harmine treatment inhibited the ability of HUVECs to organize into capillary-like networks on Matrigel in a dose-dependent manner ( Figure 5C). These results revealed that harmine inhibited neovascularization in vitro.

Harmine Inhibits Angiogenesis ex vivo and in vivo
To assess the anti-angiogenic activity of harmine, a rat thoracic aorta ring assay was performed. As shown in Figure 6A, 50 mM harmine obviously inhibited vessel sprouting from rat aortic rings.  The average area of sprouting vessels from thoracic aorta rings in the harmine-treated group was only 30%, compared with that of the control group. To further explore the effect of harmine on angiogenesis in vivo, we performed a corneal angiogenesis assay, which is an important animal model to evaluate angiogenesis. Our data showed that 5 mg harmine significantly blocked neovascularization ( Figure 6B, arrows) induced by VEGF, as measured by the vessel length, clock number and area of vessels in harmine-treated eyes, compared with those of the control group ( Figure 6C). During experimentation, we did not observe symptoms of eye inflammation including keratitis, corneal edema or advanced signs of intraocular inflammation (data not shown). Results of these two assays suggested that harmine inhibited angiogenesis ex vivo and in vivo.

Harmine Suppresses Tumor Growth by Inhibiting Tumor Angiogenesis
To determine the effects of harmine on tumor angiogenesis and growth, we performed a xenograft lung tumor assay in nude mice. After treatment for 21 days, 30 mg/kg harmine dramatically inhibited the size ( Figure 7A) and weight ( Figure 7B, left panel) of tumors, but there was no significant difference in body weight between harmine-treated mice and control mice ( Figure 7B, right panel).
To ascertain whether inhibition of solid tumor growth resulted from the decrease of new blood vessels, we examined vessel formation in harmine-treated and control groups by immunohis-tochemistry using antibodies against endothelial cell markers such as vWF and CD31. Our data indicated that the microvessel density of the harmine-treated group was much less than that of the control group ( Figure 7C), suggesting that harmine inhibited lung tumor (A549 cells) growth in a xenograft mouse model by inhibition of angiogenesis.
To further ascertain whether inhibition of angiogenesis is the only reason for the suppression of tumor growth, we examined the effect of harmine on the proliferation and migration of A549 lung cancer cells. Our results showed that harmine also inhibited the proliferation and migration of A549 lung cancer cells ( Figure 7D and 7E). These results suggested that harmine inhibited tumor growth by both inhibition of angiogenesis and inhibition of cancer cell proliferation.

Discussion
Our study as presented here identified non-peptidic carboline harmine as a novel small molecule that possessed an ability to induce p53 level and activity, consequently leading to p53 stabilization, nuclear accumulation and transcriptional activation in endothelial cells. Moreover, harmine not only inhibited angiogenesis in vitro and in vivo, but also suppressed tumor growth by inhibiting tumor angiogenesis (Figure 8). In p53 signaling pathway, p53 phosphorylation is one of the key regulatory steps for activating p53 function. In different sites, p53 was phosphorylated by several kinases such as casein kinase II (CK II), DNA-dependent protein kinase (DNA-PK), protein kinase C (PKC) and mitogen-activated protein kinase (MAPK) [39] followed by resulting in nuclear accumulation of p53 [33]. Other study showed that, in response to DNA damage, phosphorylation p53 at serines 15 and 37 by DNA-PK inhibited MDM2 binding to p53 [40]. In addition, phosphorylation of p53 at serine 20 resulted from some other kinases including PIK3 [41] is also thought to play a role in modulating the negative regulation of p53 by MDM2. In this study, we demonstrated that harmine induced p53 phosphorylation at ser-37 and ser-15 sites, but not the ser-20 site ( Figure 1C and 1D) followed by activating the p53 signals in endothelial cells ( Figure 1B). However, we did not find harmine induced obvious DNA damage ( Figure S1) measured by H2AX and comet assays. These results, together with other studies, suggested that harmine induces p53 phosphorylation possibly in response to other factors and further studies in depth will be conducted later.
Several studies have shown that the p53 activation reduces nuclear export of p53, increased transcriptional activity of p53  [42] and leads to growth arrest or apoptosis [43]. We found that harmine induced p53 stabilization (Figure 2A and 2B) and nuclear accumulation ( Figure 2C and 2D). Furthermore, we also found that harmine regulated the expression of downstream p53-target genes, including cyclin B1, cyclin A and CDC2 ( Figure 3A), which are involved in the endothelial cell cycle. On the other hand,importantly, major roles of the p53 protein in cancer had also been shown to limit angiogenesis by some mechanisms [5] including directly increasing the production of endogenous angiogenesis inhibitors and potential angiogenic markers such as Bai1 and TSP-1 [44].
Our present study demonstrated that, in the dose-dependent manner, harmine induced the expression of Bai1 and TSP-1  ( Figure 3B). These results, together with the results shown in Figure 1, suggested that harmine induced nuclear accumulation of p53 and activated p53 transcriptional activities due to reactivation of p53.
Angiogenesis is necessary of tumor growth. In this study, using a xenograft mouse model and immunohistochemistry with anti-vWF and anti-CD31 antibodies (Figure 7), we demonstrated that harmine inhibited tumor growth with low side-effects by suppressing tumor angiogenesis. A recent report [45] showed the effect of harmine on angiogenesis in vitro and tumor-specific capillary formation, but it did not demonstrate the anti-tumor activity of harmine by suppressing tumor angiogenesis. In the present study, we demonstrated that, under the effective dosage, inhibiting tumor angiogenesis may be the important aspect of antitumor activity of harmine and we expounded the novel roles of harmine in anti-tumor activity by inhibiting angiogenesis.
Some other studies showed that p53 induced the activity of PARP [46] and inhibited survival by activating p21, a regulator of apoptosis and the cell cycle [47]. In addition,VEGF/VEGFR2 independent control of the anti-apoptotic pathway in endothelial  cell is mediated by the activation of p53/caspases [48]. So, VEGF and p53 expression are significantly correlated and might be associated with relevant events involved in tumor biology [49]. In this study, we demonstrated that harmine induced endothelial cell apoptosis ( Figure 4A), cell cycle arrest ( Figure 4B) and reduced cell viability ( Figure 4C) mainly depending on p53 pathways. Furthermore, harmine not only inhibited angiogenesis ex vivo in an aorta ring assay ( Figure 6A), which is consistent with a recent report [45], but also inhibited VEGF-induced angiogenesis in vivo in a mouse corneal assay ( Figure 6B and 6C), the gold standard of angiogenesis analysis [21]. All these results suggest that harmine inhibits angiogenesis in vitro, ex vivo and in vivo.
In conclusion, our results not only indicate that harmine is a novel activator of p53 in endothelial cells (Figure 8), but also suggest that harmine may exert its biological activities on other angiogenic diseases through p53 signaling pathway.