A Spontaneous Fatp4/Scl27a4 Splice Site Mutation in a New Murine Model for Congenital Ichthyosis

Congenital ichthyoses are life-threatening conditions in humans. We describe here the identification and molecular characterization of a novel recessive mutation in mice that results in newborn lethality with severe congenital lamellar ichthyosis. Mutant newborns have a taut, shiny, non-expandable epidermis that resembles cornified manifestations of autosomal-recessive congenital ichthyosis in humans. The skin is stretched so tightly that the newborn mice are immobilized. The genetic defect was mapped to a region near the proximal end of chromosome 2 by SNP analysis, suggesting Fatp4/Slc27a4 as a candidate gene. FATP4 mutations in humans cause ichthyosis prematurity syndrome (IPS), and mutations of Fatp4 in mice have previously been found to cause a phenotype that resembles human congenital ichthyoses. Characterization of the Fatp4 cDNA revealed a fusion of exon 8 to exon 10, with deletion of exon 9. Genomic sequencing identified an A to T mutation in the splice donor sequence at the 3′-end of exon 9. Loss of exon 9 results in a frame shift mutation upstream from the conserved very long-chain acyl-CoA synthase (VLACS) domain. Histological studies revealed that the mutant mice have defects in keratinocyte differentiation, along with hyperproliferation of the stratum basale of the epidermis, a hyperkeratotic stratum corneum, and reduced numbers of secondary hair follicles. Since Fatp4 protein is present primarily at the stratum granulosum and the stratum spinosum, the hyperproliferation and the alterations in hair follicle induction suggest that very long chain fatty acids, in addition to being required for normal cornification, may influence signals from the stratum corneum to the basal cells that help to orchestrate normal skin differentiation.


Introduction
Keratinocytes in the mammalian epidermis are stratified into four cellular layers: stratum basale (basal), stratum spinosum (spinous), stratum granulosum (granular), and stratum corneum (cornified). The basal cells are proliferative and express characteristic markers, including keratins 5 and 14. The spinous cells have withdrawn from the cell cycle and express keratins 1 and 10. The granular cells synthesize lamellar bodies/keratohyalin granules, and then convert to corneocytes, which are enucleated and encapsulated by a modified plasma membrane termed the corneocyte envelope (CE). The CE protects against water loss (an inside-outside barrier) and against insults such as microbes from without (an outside-inside barrier) [1,2]. The lipid matrix of the CE contains ceramides, long chain fatty acids, and cholesterol and its esters, which are deposited from the lamellar bodies of the granular cells. During epidermal development in mammals, defects in the production of structural proteins, or enzymes, or lipid components of the CE result in barrier defects and/or congenital ichthyoses [2,3].
Mammalian very-long-chain acyl-CoA synthetases (ACSVLs) or fatty acid transport proteins (FATPs) are a family of six related proteins [4]. These proteins contain two ''signature'' domains: the ATP/AMP domain which is required for ATP binding, and the VLACS/FATP domain (approximately 50 amino acids), which is required for fatty acid binding and enzymatic activity [5,6]. The FATP genes have different expression patterns, and the proteins have different sub-cellular locations and substrate specificities. Defective ACSVLs/FATPs have been implicated in human diseases such as heart failure, obesity, diabetes/insulin resistance, cold intolerance, and fat mal-absorption [4,7]. Furthermore, the most widely expressed member of this family is Fatp4, which is encoded by Slc27a4 (solute carrier family 27 member 4 gene), and its broad expression pattern is suggestive of functions in many organs [8,9]. In mammalian skin, Fatp4 protein is localized to the stratum granulosum and the stratum spinosum [9][10][11].
The physiological role of Fatp4 has been studied using mouse models. A retrotransposon insertion into exon 3 of Fatp4 was identified in an autosomal recessive mouse mutant termed wrinklefree (wrfr) [12]. Independently, a targeted knock-out of Fatp4 (that affects exon 3) was generated and characterized [10]. In both cases, mutant mice are born with tight, thick, shiny skin and a defective skin barrier [10,12]. The mutant mice die shortly after birth. In a third mouse model, deletion of Fatp4 exons 2 and 3 was found to result in embryonic lethality prior to embryonic day 9.5 [13]. The reason for this discrepancy remains unknown. Fatp4 has also been conditionally deleted in the adult mice [9]. By gross appearance these mice appear normal, but mild histological abnormalities are present in the epidermis, supporting a role for Fatp4 in skin homeostasis [9]. Using a transgenic approach, expression of Fatp4 in suprabasal keratinocytes was found to be sufficient to rescue the wrfr mutant phenotype, resulting in viable and fertile mice [8]. The Fatp4 mutant mice were initiatively suggested to be a mouse model for a very rare human genetic disorder, lethal restrictive dermopathy [1,10,12,14]. Restrictive dermopathy in humans has now been linked to mutations in the zinc metalloproteinase ZMPSTE24 whereas mutations in Fatp4 in humans cause ichthyosis prematurity syndrome (IPS) [11,15]. IPS is a rare disorder of cornification classified as one of the autosomalrecessive congenital ichthyoses [16]. Key features in IPS are complications resulting from prematurity born with thick caseous desquamating epidermis, typically showing lipid membrane packages in the granular and cornified cells, then a lifelong nonscaly ichthyosis with dermal atopic dermatitis-like inflammation and severe itching [17].
In the current study, we describe the identification and characterization of a spontaneous mutation in mouse Fatp4 that results in autosomal recessive congenital ichthyosis. At birth, the mutant mice have smooth hyperkeratotic skin that is stretched so tightly that they are unable to extend their limbs or to straighten their torso. Histological studies revealed defects in epidermal differentiation and cornification. The mutation was mapped to chromosome 2, band A3/B, by SNP analysis, thus suggesting Fatp4/Slc27a4 as a candidate gene. Sequencing studies revealed a spontaneous mutation in the splice donor sequence at the 39-end of exon 9, resulting in exon skipping, a shift in reading frame, and the presence of a premature stop codon. The mutation results in loss of the C-terminal 243 amino acids of Fatp4, including the VLACS domain. The Fatp4 mutant mice exhibit alterations in the stratum corneum that are similar to the defects seen in IPS [11], presumably reflecting a role for very long chain fatty acids in the formation and function of lamellar bodies. The Fatp4 mutants also show basal cell hyperproliferation and a reduction in secondary hair follicle induction, suggesting the possibility that very long chain fatty acids synthesized in the superficial epidermis may, directly or indirectly, help to establish the proper prenatal balance between proliferation and differentiation of the basal cells.

Histological Analysis and Immunofluorescence
Skin from newborn mice was fixed in 10% neutral buffered formalin (NBF), and embedded in paraffin. Sections were cut at 5 um and stained with hematoxylin/eosin. For in vivo BrdU incorporation, newborns were injected i.p. with 250 mg/g BrdU (Sigma) in 0.9% sterile saline as described [20]. After 1 h, tissue was fixed in 10% NBF. Primary antibodies used for immunofluorescence were FITC anti-BrdU (Becton Dickinson, Franklin Lakes, NJ), guinea pig anti-K14 [21], rabbit anti-K1 [21], and  rabbit anti-K6 [22]. Secondary antibody conjugates used were Alexa594-conjugated goat anti-guinea pig and 488-conjugated goat anti-rabbit (Molecular Probes). All experiments involving mice were approved by the Baylor IACUC.  [11,[28][29][30][31]. Aa, amino acids and their position in the protein; bp, nucleotide changes and their location relative to the start codon; the exon name is listed last for each mutation. B. Pigskin Fatp4 protein, cDNA sequencing results and the encoded amino acid sequence. The mutant protein is predicted to be 449 aa in length, with the last 49 aa (in red) encoded after a frameshift caused by the loss of exon 9. The truncated Fatp4 protein is missing the FATP/VLACS domain. Exon 8 nucleotides and wt amino acids are in black. Exon 10 nucleotides are in light blue and exon 11 nucleotides are in purple. The black triangles indicate exonic boundaries. C. Schematic drawing of altered splicing plus the genomic sequencing results. The pigskin mutants have a point mutation (A to T) in the splice donor site for Slc27a4 exon 9. D. Lack of full-length Fatp4 protein in the skin of pigskin mutant mice. A Western blot analysis was performed using an anti-Fatp4 antibody generated against a C-terminal peptide antigen. Lane 1 is a positive control (10 ug cell lysate from 293T cells transfected with mouse Fatp4 cDNA (NM_011989)). Lane 2: 25 ug whole skin lysate from a pigskin newborn. Lane 3: 50 ug whole skin lysate from a normal littermate. Lane 4: 50 ug whole skin lysate from a pigskin newborn. Beta-actin was used as a loading control. doi:10.1371/journal.pone.0050634.g005 Figure 6. Alteration of skin structure at the earlier stages of pigskin mutants. Hair follicle induction was assayed using a BMP4-lacZ reporter line from E14.5 to E16.5. Embryos were incubated in solution with 5-bromo-4-chloro-3-indolyl-b-D-galactoside (X-gal). X-gal cleavage by betagalactosidase results in dark blue staining. A. At E14.5, representative control and pigskin mutant embryos display blue-stained primary hair follicles (PHFs) and vibrissal follicles. There were similar numbers of PHFs in the lateral side of control and mutant mice. B. Peeled skin from representative control and pigskin E16.5 embryos was stained with X-gal. Primary hair follicles (PHFs) are larger and often show an unstained core and a distinctive ring shape (red arrows). Secondary hair follicles (SHFs) are smaller and are more numerous. The ratio of SHFs to PHFs in the mutant epidermis is significantly decreased compared to that of the control (n = 3, p,0.01, see materials and methods). C, D. Intact E15.5 control embryos display bluestained hair follicles over most of their surface, except for local regions on the dorsum with limited staining (black arrow) (C). In contrast, pigskin mutant embryos had large portions of their back and lateral skin as well as ventral sites (white arrow) that were not stained by X-gal, indicating alternated permeability at E15.5 (D). doi:10.1371/journal.pone.0050634.g006

Western Blot Analysis
Newborn dorsal skin was homogenized and extracted in lysis buffer (10 mM Tris-Cl at pH 7.4, 5 mM EDTA, 100 mM NaCl, 1% Triton X-100 with Complete Proteinase Inhibitors Cocktail) (from Roche). 293T cells (human kidney cells) were grown in supplemented DMEM medium (Invitrogen), and transfected with an expression construct encoding mouse Fatp4 (NM_011989) (purchased from Open Biosystems) using FuGene6 (Roche). Cells were harvested for Western blot analysis 48 h after transfection. The primary antibody (1:500) was a rabbit antibody generated against the C-terminal 35 amino acids of mouse Fatp4, a gift from Dr. Paul A. Watkins (Kennedy Krieger Institute) [23]. After incubation with an HRP-conjugated anti-rabbit secondary antibody, protein bands were visualized using Super Signal West Pico Substrate (Pierce). An antibody against beta-actin (Sigma-Aldrich, cat#: A2228) was used as a loading control.

X-gal Staining of Whole Embryos and Skin
To assess the pattern of hair follicle induction, we used a BMP4-lacZ reporter line [24] and we assayed for ß-galactosidase activity by X-gal staining as described previously [25]. Briefly, males that were compound heterozygous for the Fatp4 mutation and for BMP4-lacZ, were mated to females heterozygous for the Fatp4 mutation. Embryos were genotyped by PCR using one pair of primers to amplify the wild type allele (Ex8 (S), 59-CCACT-GAATG CAACTGTAGCC-39 and Ex9(WT,AS), 59-TCCATTCCCTCCTGGGCAGACCT-39 and a different antisense primer (Ex9, pigskin AS, 59-TCCATTCCCTCCTGGG-CAGACCA-39 to assay for the mutant allele. Amplification bands were 360 bp. Mouse embryos or peeled skin were harvested from timed pregnancies and fixed in 2% paraformaldehyde plus 0.2% glutaraldehyde in 0.1 M phosphate buffer (pH 7.3) at 4uC for 1 hour. Embryos or skin were rinsed three times (30 minute each) in washing solution containing 0.1 M phosphate buffer (pH 7.3), 2 mM MgCl2, 0.01% sodium deoxycholate, and 0.02% NP-40. Embryos were then stained at 4uC for 12 hours in X-gal staining solution (washing solution plus 5 mM potassium ferrocyanide, 5 mM potassium ferricyanide, and 1 mg/mL X-gal). Stained embryos or skin were rinsed in phosphate-buffered saline (PBS; pH 7.4) and stored in 70% ethanol. After staining, embryos were photographed using a 35 mm Nikon digital camera and images were processed with Adobe Photoshop. All of blue hair follicles in the lateral body (1 mm x 1 mm area) of E14.5 embryos were counted (at least three embryos in each genotype). A strongstained blue dot with an unstained core and a distinctive ring shape from the skin of E16.5 embryos was counted as primary hair follicles (PHFs) while other smaller stained blue dots were counted as secondary hair follicles (SHFs). Statistical significance (p values) was computed by using Student's t test. A p value of less than 0.05 was considered statistically significant. Image J software was used to count hair follicles [26].

New Mouse Mutant with Autosomal Recessive Congenital Ichthyosis
Within a breeding colony of FVB mice, a few of the offspring from one breeder pair exhibited an abnormal skin phenotype at birth. Some pups were born with tight, smooth, shiny skin ( Fig. 1A and B). The skin was stretched so tightly that the newborns were immobilized in a fetal position, unable to extend their body or their limbs. The characteristic appearance of the skin led us to describe the newborns as ''pigskin'' mutants. The mutant mice had a small jaw and protruding tongue (Fig. 1A). In some mutants, the toes and the tip of the tail showed signs of necrosis at birth (not shown). Although some of the mutants were able to breathe, they died shortly (within a few hours) after birth. We found no milk in their stomachs, indicating they were unable to suckle. Stretching of the skin caused widespread cracking (Fig. 1C), reminiscent of congenital ichthyoses in humans [11,27]. Breeding studies confirmed that the pigskin phenotype was inherited as an autosomal recessive trait.

Aberrant Epidermal Differentiation and Hyperkeratosis
Skin from newborn mice was harvested and processed for histological analyses. The exterior surface of the skin and the epidermal-dermal junction were flattened compared with normal skin (Fig. 2A). The mutant epidermis was notably thicker than normal. The stratum corneum of the mutant epidermis was considerably thicker than control epidermis ( Fig. 2B and 2C), indicative massive hyperkeratosis (abnormal accumulation of cornified cells). The cells of the stratum granulosum showed changes in the patterning, size, and distribution of the dense basophilic keratohyalin granules in the mutant skin (Fig. 2C, white arrows). These granules contain aggregated keratin fibers and lipids, which help to build the epidermal barrier. The stratum spinosum was characterized by an increased number of cell layers in the mutant. No significant abnormalities were identified in other tissues (data not shown).
To investigate proliferation and differentiation of the keratinocytes, we employed keratin immunostaining and BrdU incorporation assays (Fig. 3). In control skin, Keratin K14 expression is detected in the basal epithelial cells while keratin K1 reactivity was observed in all suprabasal cell layers (Fig. 3A). The mutant epidermis showed K14 labeling in more suprabasal layers (Fig 3A  and 3B). BrdU-labeled cells were detected sporadically in the stratum basale in control epidermis, but more than twice as many BrdU-labeled cells were found in the mutant epidermis (Fig. 3B). We also assayed the epidermis for expression of Keratin K6, a marker of aberrant epidermal differentiation. K6-labeled cells were strongly detected in the suprabasal layers of the mutant epidermis, but not in the control epidermis (Fig. 3C). These findings indicate that all layers of the skin are affected in the pigskin mutant.

SNP Mapping of the Pigskin Mutation
The pigskin mutation arose on an FVB background. In order to map the mutation, we mated pigskin carrier males to C57BL/6J partners. The F1 offsprings were used for test matings to identify mice that carried the pigskin mutation. Carriers were mated to each other, and the F2 offspring were again mated to identify carriers of the pigskin mutation. F2 carriers and their mutant offspring were used for SNP analysis [19]. We analyzed genomic DNA from four carrier parents, and nine affected newborns (Fig. 4) as well as the parental FVB and C57 lines. SNP mapping identified a candidate region of the genome centered around rs13459062, which is located near 30 Mb on mouse chromosome 2.
Since Fatp4 (Slc27a4) maps at 29.5 Mb on chromosome 2, it lies within the critical region of the genome. Mouse Fatp4 and human FATP4 encode homologous proteins. Recent studies have identified mutations in FATP4 in human patients with IPS [11,[28][29][30][31] and the mutations found so far are point mutations (summarized in Figure 5A). In order to look for defects in the Fatp4 coding sequences in the pigskin mutants, segments of the mRNA were amplified by RT-PCR from newborn mutant and wild-type skin (see Materials and Methods). Using an exon 4 sense primer and an antisense primer from exon 9, wild-type skin gave an amplification band, while the mutant skin did not (data not shown). Using a sense primer from exon 8 and an antisense primer from exon 11, the mutant RNA gave an amplification band that was about 120 bp smaller than wild-type (data not shown). Sequencing revealed that exon 9 was completely missing from the mutant transcript (Fig. 5B). The loss of exon 9 (127 bp) causes a shift in the coding frame so the pigskin transcript encodes a truncated protein with only 449 amino acids. Of these, only the first 400 amino acids are from wild-type Fatp4 (see Fig. 5B). The truncated protein will be missing the conserved VLACS/FATP domain. Based on the RT-PCR results, we designed primers to amplify exon 9 and the flanking genomic sequences by direct PCR from genomic DNA. Sequencing of the amplified bands revealed a point mutation (an A to T transversion) in the consensus splice donor sequence at the 39end of exon 9 in the mutant genome (Fig. 5C). Since antibodies against the N-terminus of Fatp4 are not available, westerns were performed using a Fatp4 antibody generated against a peptide from the C-terminus of Fatp4 (Fig. 5D). No band was detected in extracts from mutant skin (Fig. 5D, lanes 2 and 4), verifying the prediction that the full length Fatp4 protein is not synthesized by the pigskin mutants. Together, we conclude that this point mutation in Slc27a4 is the cause of the pigskin phenotype.

Alternated Hair Follicle Growth and Skin Structure at the Earlier Stages of Pigskin Embryos
The shiny and smooth phenotype of the newborn pigskin mutant skin and our histological studies suggested that there might be a defect in hair follicle induction, consistent with the previous report that the wrfr mutant mice had impaired hair development with fewer developing hair follicles. In mice, tylotrich or primary hair follicles (PHFs) are induced beginning at E14 and are characterized by a large hair bulb and two sebaceous glands [32][33][34]. Nontylotrich or secondary hair follicles (SHFs) begin to differentiate at approximately E16 [33]. In order to assess whether PHFs or SHFs or both are affected in the mutant mice, we intercrossed pigskin mice with a BMP4-lacZ reporter strain (from Dr. Yas Fututa) [24]. These mice have a lacZ reporter inserted into the endogenous BMP4 locus by homologous recombination [24]. BMP4 expression is robust at the onset of primary and secondary hair follicle induction [35,36]. Interestingly, at E14.5, the pigskin mutants showed a similar pattern, distribution and density of PHFs compared to the control (Fig. 6A). There were no significant changes of PHFs numbers in the control and mutant mice (the numbers per square millimeter: wt = 23.562.1, pigskin = 24.663.2, n = 3, p = 0.57). However, at E16.5, skin from the pigskin mutants showed a significant decrease in the numbers of hair follicles compared to the control littermates (Fig. 6B). Notably, the ratio of SHFs to PHFs in the mutant epidermis is significantly decreased (the ratio of SHFs to PHFs: wt = 3.0160.20, pigskin = 1.2760.28, n = 3, p,0.01). Thus, the Fatp4 mutation leads to a reduction of number of SHFs.
Skin barrier formation follows a precise spatiotemporal pattern during embryogenesis [37]. The timing of development of the epidermal barrier can be examined by incubation of embryos in X-Gal solution at low pH. Detection endogenous beta-galactosidase activity occurs only prior to development of the barrier. Using this approach, previous studies demonstrated that Fatp4 mutant skin has a barrier defect at E18.5 and in newborn mice [9,10,12]. In our study, when we performed X-gal staining to detect the BMP4-LacZ activity in hair follicles, we found that X-gal penetrated the epidermis and stained the hair follicles in both control and mutant embryos at E14.5 (Fig 6.A). However, interestingly, at E15.5, blue staining was almost completely absent from the dorsal skin of the mutant embryos, but was observed throughout the dorsum of the control littermates (Fig. 6C). Blue staining was observed in the ventral follicles in the control embryos, but was partially lost on the ventral side of the mutants (Fig. 6D, arrow). At E16.5, both control and mutant embryos had no X-gal staining of intact dorsal and lateral skin (data not shown). Together, these data suggest that skin barrier development has been affected by E15.5 in the mutant embryos.

Discussion
We have identified and characterized a new mouse model for autosomal recessive, non-bullous, congenital ichthyosis. Mutant mice are born with a ''tight skin'' phenotype. The skin is stretched so tightly that the mice are unable to move their limbs. The mutant mice have a distinctive protruding tongue (Fig. 1A), are unable to suckle, and die shortly after birth. Histological analysis of the skin showed hyperkeratosis ( Fig. 2A-C), defects in the lamellar bodies of the granular cells (Fig. 2B,C), hyperproliferation (Fig. 3B) and altered of keratin marker gene expression (Fig. 3A-C). The mutation was mapped using SNP analysis (Fig. 4). Molecular characterization of the Fatp4/Slc27a4 transcript (Fig. 5B) and gene (Fig. 5C) identified a point mutation in the splice donor sequence of exon 9, resulting in exon skipping during processing of the primary transcript. The altered transcript encodes a predicted truncated Fatp4 protein that lacks the conserved VLACS domain (Fig. 5C). Western blots confirmed the loss of expression of the fulllength protein in newborn skin (Fig. 5D). This spontaneous mutation provides further evidence that Fatp4 is essential for proper cornification and barrier formation in the epidermis.
Currently, autosomal recessive congenital ichthyosis (ARCI) in humans is associated with mutations in genes including TGM1  [38]. Among them, mutations in ABCA12, a member of the ABC transporter superfamily, cause Harlequin ichthyosis (HI), a disorder that presents at birth with a thick, tight skin that is susceptible to cracking [1]. In keratinocytes, ABCA12 is thought to regulate the transfer of glucosyl-ceramides into lamellar bodies. Loss of ABCA12 function in mice causes hyperkeratosis (expanded stratum corneum) and malformed lamellar bodies [39]. These phenotypes resemble the ''pigskin'' phenotype in the Fatp4 mutant mice [10,12]. Like ABCA12, Fatp4 plays an essential role in the construction or function of lamellar bodies [10]. Fatp4 functions as an acyl-CoA synthetase with specificity towards very long chain fatty acids including arachidonate (C20:4) and lignocerate (C24:0), which are essential for lamellar bodies [4]. We therefore predict that Fatp4 and ABC12 may cooperate either directly or indirectly in lamellar bodies to help produce the normal cornified envelope (CE). Mutations of TGM1 (keratinocyte transglutaminase 1), a calcium dependent enzyme that functions in cross-linking of epidermal structural proteins and lipids into the CE, cause lamellar ichthyosis [40]. Studies have showed that expression of TGM1 is directly regulated through its promoter by GRHL3/ GET1, an epidermal-specific transcription factor [41,42]. It will be interesting to know whether GRHL3/GET1 also regulates Fatp4 expression during epidermal development. In the future, determination of the upstream regulators of Fatp4 expression and its interaction with other proteins mutated in ARCI may give us insights into the molecular events that specify the unique architecture of the CE.
Recently, human mutations of FATP4 have been found to cause IPS, a well-defined congenital ichthyosis subtype [11,[28][29][30][31]. Klar et al first reported FATP4 mutations in IPS patients from the Scandinavia, Middle East and North Africa [11]. IPS is more prevalent in Norway and Sweden with an estimated local carrier frequency of one in 50 suggesting a founder mutation. Outside of this region, only a few cases have been reported in other countries including Germany, Finland, Italy, Denmark and France. All patients from this region were found homozygous or compound heterozygous for p.C168X nonsense mutation. Up to now, thirteen distinct FATP4 mutations have been found in IPS patients (see summary in Figure 5A), including two nonsense mutations, eight missense mutations, one start site mutation, and two splice site mutations [11,[28][29][30][31]. Notably, all of patients with IPS were reported to present similar clinical features. In our case, a point mutation (an A to T transversion) in the consensus splice donor sequence at the 39-end of exon 9 results in exon skipping and predicts synthesis of a truncated protein without the FATP/ VLACS motif. The consensus genomic 59 splice sequence in mammals, from 23 to +6 relative to the exon/intron boundary, is 59-CAGGTAAGT. This sequence binds with perfect complementarity to the U1 snRNA. Although A is the nucleotide found most often at position 22 (64% of the time), T is present 14% of the time, so the presence of a T does not intrinsically prevent efficient splicing [43][44][45]. Looking specifically at intron 9 of mouse Slc27a4, the wild-type genomic sequence at the 59-exon/intron boundary is 59-CAGGTctGc. Six of these nine nucleotides match the consensus. In the pigskin mutant, the change of A to T at position 22 leaves only 5 nucleotides that match the consensus. Our findings imply that this change is sufficient to prevent effective use of this splice site.
The ''pigskin'' mutant mice display a comparable phenotype to the wrfr and Fatp4 knockout mice described in previous studies [10,12]. However, the wrfr mutation was caused by a 230 bp retrotransposon insertion into Exon3 and the knockout mice were generated by deleting a genomic fragment containing exon3. Thus, the ''pigskin'' mice may be particularly useful to develop molecular therapies for IPS patients using targeted gene correction [46].
Since Fatp4 protein is detected specifically in suprabasal cells [10] and targeted expression in those cells is sufficient to rescue the mutant phenotype [8], we hypothesize that the basal cell hyperproliferation, the abnormal expression of K6, and the alterations in secondary hair follicle induction in Fatp4 mutants all reflect indirect, non-cell autonomous, responses to the loss of synthesis and release of very long chain fatty acid derivatives from the spinous and granular cells. We hypothesize that very long chain fatty acids synthesized by Fatp4 may provide both metabolic and regulatory functions that help to modulate epidermal homeostasis and differentiation.
In summary, we have identified a new mouse model for autosomal recessive congenital ichthyosis. The pigskin mutant mice, like most human patients with IPS, have a point mutation in the gene encoding Fatp4. These new mice provide a potential model system in which to study the feasibility of achieving gene therapy in the epidermis using homology-based strategies to correct single base mutations.