CXCL10 Is Critical for the Generation of Protective CD8 T Cell Response Induced by Antigen Pulsed CpG-ODN Activated Dendritic Cells

The visceral form of leishmaniasis is the most severe form of the disease and of particular concern due to the emerging problem of HIV/visceral leishmaniasis (VL) co-infection in the tropics. Till date miltefosine, amphotericin B and pentavalent antimony compounds remain the main treatment regimens for leishmaniasis. However, because of severe side effects, there is an urgent need for alternative improved therapies to combat this dreaded disease. In the present study, we have used the murine model of leishmaniasis to evaluate the potential role played by soluble leishmanial antigen (SLA) pulsed-CpG-ODN stimulated dendritic cells (SLA-CpG-DCs) in restricting the intracellular leishmanial growth. We found that mice vaccinated with a single dose of SLA-pulsed DC stimulated by CpG-ODN were protected against a subsequent leishmanial challenge and had a dramatic reduction in parasite burden along with the generation of parasite specific cytotoxic T lymphocytes. Moreover, we demonstrate that the induction of protective immunity conferred by SLA-CpG-DCs depends entirely on the CXC chemokine IFN-γ-inducible protein 10 (CXCL10; IP-10). CXCL10 is directly involved in the generation of a parasite specific CD8+ T cell-mediated immune response. We observed significant reduction of CD8+ T cells in mice depleted of CXCL10 suggesting a direct role of CXCL10 in the generation of CD8+ T cells in SLA-CpG-DCs vaccinated mice. CXCL10 also contributed towards the generation of perforin and granzyme B, two important cytolytic mediators of CD8+ T cells, following SLA-CpG-DCs vaccination. Together, these findings strongly demonstrate that CXCL10 is critical for rendering a protective cellular immunity during SLA-CpG-DC vaccination that confers protection against Leishmania donovani infection.


Introduction
Among the various antigen presenting cells (APCs) of the immune system, dendritic cells (DC) are critical for the adaptive immune response as they acts as conduits between the innate and adaptive arms of the immune system [1]. Exogenous administrations of antigen-loaded DCs have shown promising results in the treatment of visceral leishmaniasis [2,3,4]. However, the roles of toll like receptor ligand-activated DCs in the containment of L.donovani infection needs further elucidation. Toll like Receptors are a type of germ-line, pattern recognition receptors that recognize pathogen-associated molecular patterns (PAMPs) [5]. Therefore, TLR activation on DCs helps in mounting a more prominent and directed T cell response [6] and superior killing of the invading pathogen.
CpG oligodeoxynucleotides (ODN) containing unmethylated CpG motifs serve as TLR9 ligands can stimulate DC activation and maturation to professional APCs [7,8]. CpG-ODN enhances humoral responses, driving them toward IgG2a isotypes, a Th1 type indicator [9], and induces development of enhanced cytotoxic T lymphocyte (CTL) activity [10] by cross-presenting extracellular antigens to CTLs which destroy the antigen-specific pathogens in vivo [11]. TLR ligand-stimulation of DCs causes the production of chemokines, creating gradients that attract naive CTLs and increase the possibility of their encounter with the DCs [12]. Chemokines (8)(9)(10) are an integral part of the host defence against pathogens and have been subdivided into 2 major subfamilies, CC and CXC chemokines [13,14]. Amongst the latter, CXCL10 is produced by dendritic cells and various others activated human immune cells following CpG-ODN stimulation [15,16]. CXCL10, with known antitumor, antiviral, and antifungal activities [17][18][19], is essential for the generation of protective CD8 + T cell responses [20]. An early and strong induction of CXCL10 accompanies healing in L.donovani infected B6 mice, while treatment with exogenous CXCL10 renders protection against L. donovani infection, highlighting its relevance in aborting the leishmanial pathogenesis [21,22]. CXCL10 binds to CXCR3, a seven-transmembrane G protein-coupled receptor expressed on T cells that induces chemotaxis [23]. CXCR3 is critical for T cell activation [24]. CXCR3 2/2 mice are highly susceptible to infection due to impaired perforin and granzyme B expression by CTLs associated with reduced cytotoxic activity [25]. Collectively, these observations stimulated us to determine the role of CXCL10 in TLR ligand-activated DC based vaccinations, as it can promote the activation of parasite specific CD8 + CTLs in vivo.
This study demonstrates for the first time, that the induction of an anti-leishmanial CD8 + T cell response, following SLA pulsed-CpG-ODN stimulated DC based vaccination is entirely dependent on the production of CXC chemokine CXCL10. Studies in a murine model of visceral leishmaniasis showed that CXCL10 plays a key role in generating parasite-specific-CTLs following SLA-CpG-DC vaccination along with effective killing of the parasite. This study might provide crucial cues in understanding the immunostimulatory role of SLA-CpG-DCs in rendering protection against experimental VL.

Ethics Statement
This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All experimental animal protocols received prior approval from the Institutional Animal Ethical Committee (Bose Institute, Registration Number: 95/99/CPCSEA).

Preparation of Dendritic Cells
Bone marrow-derived DCs from BALB/c mice were generated described previously [26]. Nonadherent cells were collected, and 1610 6 cells were placed in plates containing 1 ml of complete medium with GM-CSF (150 U/ml; R&D systems) and IL-4 (75 U/ml; R&D systems). Half of the medium was replaced on day 3, 5 and 7 and fresh medium containing GM-CSF and IL-4 was added. On day 8 of culture, most cells had acquired typical dendritic morphology. These cells were used as the source of DCs in subsequent experiments.

DC Vaccination
For DC-based vaccination four sets were used. DCs were pulsed with either SLA for 18 hours or CpG-ODNs for 18 hours or with both SLA and ODNs (CpG-ODNs or control-ODN) [3]. In case of dual stimulation, CpG-ODN (10 mg/ml) or control-ODN (10 mg/ml) were added to the media for last 6 hours after 12 hours of SLA stimulation. DCs were then washed with PBS thrice and injected i.v. (10 6 cells in 100 ml of PBS/mouse) into mice through the tail vein. One week later, mice were infected intravenously with 1610 7 stationary phase L. donovani promastigotes. Mice were sacrificed on days 1, 7, 14, 28, and 56 post infections. Spleen and liver parasitic loads were determined from giemsa-stained impression smears, calculated as the number of parasites per 1000 nucleated cells x organ weight (in mg) and expressed in Leishman Donovan Units (LDU) [27]. For reinfection study mice were infected intravenously with 1610 7 stationary phase L. donovani promastigotes 4 weeks after the original infection. Mice re-infected were sacrificed at 12-wk of initial infection and organ parasite burden was determined as above.

In vivo Depletion of CXCL10 and CD8 + T Cells
For in vivo depletion of CXCL10, anti-mouse CXCL10 mAb (R&D Systems) were injected intraperitoneal (i.p.) on day 0 (250 mg) day 2 (100 mg) and day 4 (100 mg) after SLA-CpG-DCs vaccination. These mice were subsequently infected with 1610 7 stationary phase L. donovani promastigotes after 7 days of initial vaccination. 250 mg of anti-CXCL10 mAb was again injected i.p on days 10, 15, and 24 of initial vaccination. CD8 + T cells were depleted by one i.p. injection of CD8 depleting antibody (clone TIB210) (500 mg) 1 day before the vaccination. Depletion efficiencies were assessed at regular intervals with mAbs by flow cytometric analysis.
Purification of CD8 + T Cells CD8 + T cells were purified from splenocytes from differently treated mice by positive selection using magnetic beads (Mouse CD8 T Lymphocyte Enrichment Set; BD Biosciences). The CD8 + population purity was routinely confirmed to be around 98%.

Proliferation Assay
Splenic CD8 + T cells (10 6 ) were cultured for 4 days in 96-well, round-bottom plates. SLA (10 mg/ml) or ConA (2.5 mg/ml) was added to the culture medium for stimulation for 72 hours. One mCi of [ 3 H] thymidine (JONAKI, DAE) was added 18 h before harvest and incorporated radioactivity was measured using a liquid scintillation counter (Tri-Carb 2100TR; Packard Instrument).

Cytokine and Chemokine ELISA
Culture supernatants were analyzed using a sandwich ELISA kit (Quantikine M; R&D Systems), in accordance with the manufacturer's instructions. Briefly, 50 mL of Assay Diluent (10% FCS in PBS) was added in ELISA plates coated with capture antibody. The plates were then incubated for 1 hr at RT followed by subsequent washing with wash buffer (0.05% Tween 20 in PBS). Then 100 mL of samples were added in respective plates and were incubated at RT for another 2 hr. After completion of incubation, the wells were aspirated and washed followed by addition of respective antibody conjugate (Detection Ab + SAv-HRP). The plates were then incubated for 2 hr at RT. After washing 100 mL substrate solution was added to each well. The plates were then kept in dark for 30 minutes. Finally 100 mL Stop Solution was added to each well and reading was taken within 30 minutes using a microplate reader set to 450 nm.

Flow Cytometry Analysis and Abs
For FACS analysis, DCs or CD8 + T cells(1610 6 ) were prepared as described elsewhere (22). DCs were stained with fluorescently labelled Abs anti-CD11c-PE, anti-CD80-PE, anti-CD86-PE, and anti-MHCII-PE in separate sets for 60 min at 4uC in dark and washed three times before acquisition. Similarly, purified CD8 + T cells were stained with anti-Granzyme B-PE and anti-Perforin-FITC Abs for 60 min at 4uC in dark. Intracellular cytokine staining of purified CD8 + T cells with anti-IFN gamma-PE was performed using a Cytofix/Cytoperm-Plus Kit with GolgiPlug (BD Pharmingen) as per the manufacturer's instructions. Cells were then washed and analyzed using a FACS calibur (BD Biosciences). A minimum of 10,000 events was collected for each sample, and data analysis was performed with the CellQuest software (BD biosciences).

Isolation of RNA and RT-PCR
RNA was isolated according to the standard protocol using TRIZOL TM reagent (SIGMA) from purified CD8 + T cells isolated from different groups of mice. Isolated total RNA was then reverse transcribed using Revert Aid TM M-MuLV Reverse Transcriptase (Fermentas). The resulting complementary DNA was used for reverse transcriptase PCR with Perkin Elmer Gen Amp PCR 2400 system. PCR amplification was conducted in a reaction volume of 50 ml with 0.5U of Taq polymerase for 35 cycles (denaturation at 94uC for 30 s, annealing at 58uC for 30 s, and extension at 72uC for 30 s. The PCR-amplified product was subsequently size fractioned on a 1% agarose gel, stained with ethidium bromide,

Cytotoxicity Assay of CD8 + T Cells
To assess the cytotoxicity of antigen-primed CD8 + T cells, purified CD8 + T cells (2610 5 ) were isolated from SLA-CpG-DCs vaccinated and CXCL10 depleted SLA-CpG-DCs vaccinated L. donovani infected mice 28 days following infection and were cocultured with autologous L.donovani-infected macrophages in a 10:1 cells /ml) were stimulated with SLA (10 mg/ml) or CpG-ODN (10 mg/ml) alone or in combinations for 24 hours. In some experimental sets, DCs were transfected with TLR9-specific siRNA or control siRNA. Briefly, DCs (10 6 cells) were transfected with TLR9 siRNA at a final concentration of 100 nM, using transfection reagent Oligofectamine (Invitrogen, Carlsbad, CA, USA) as per manufacturer's instructions. The DCs were incubated with the transfection complexes in RPMI without serum for 6 h followed by another 18 h with 10% FBS. Control cultures were transfected using scrambled TLR9 siRNA (100 nM). Cells were then washed, and treated with SLA and CpG-ODN. Cell supernatants were then obtained for detecting CXCL10 levels by ELISA. Data are means 6 standard deviations of values from 3 independent experiments conducted in the same way that yielded similar results. **P,.001, compared to unstimulated control. n.d. not detectable. doi:10.1371/journal.pone.0048727.g001 ratio. After 4 h, the co-cultured cells were harvested and stained with anti-CD14-FITC antibodies to select the target macrophages. They were also analyzed by propidium iodide staining to detect the killed macrophages in flow cytometer.

Statistical Analysis
A minimum of five mice were used per group for in vivo experiments. The data, represented as mean 6standard deviation (SD), is from one experiment, which was performed at least three times. Student's t test was employed to assess the significance of the   differences between the mean values of control and experimental groups. A P value of 0.005 was considered significant and less than 0.001 was considered highly significant.

SLA-pulsed DCs Exposed to CpG ODN Cures Established Murine Visceral Leishmaniasis
To establish whether matured antigen-primed DCs could generate a host-protective immune response in L.donovani donovani-susceptible BALB/c mice, DCs were generated. The purity of these DCs was found to be above 98% ( Figure 1A) and these DCs expressed high amount of MHC class II and co-stimulatory molecules CD80 and CD86 following in vitro stimulation with CpG-ODN ( Figure 1B). We also observed that in vitro stimulation of DCs with CpG-ODN resulted in the significantly high production of CXCL10, a CXC chemokine. However, pulsing these DCs with both CpG-ODN and SLA did not cause any increase in the secretion of CXCL10 suggesting that SLA has no role in IP-10 production by these DCs. There was significant reduction of CXCL10 level in TLR 9 silenced condition; thereby highlighting the role of TLR9 in CpG-ODN induced CXCL10 generation ( Figure 1C). Besides, the control siRNA did not have any reducing effect on the production of CXCL10 ( Figure 1C).
To evaluate the maturation requirements enabling DCs to generate a protective immune response differently pulsed DCs were subsequently injected into BALB/c mice which were challenged with L. donovani intravenously 1 week later, and the course of disease was monitored. Our results demonstrated that when DCs were SLA-pulsed in the presence of a prominent Tolllike receptor (TLR) agonist, CpG ODN (SLA-CpG-DCs), they acquired the ability to induce protection in susceptible BALB/c mice against an otherwise lethal infection with L. donovani. SLA-CpG-DCs vaccinated mice on day 28 (4 weeks) of infection, showed marked decrease in parasitic burden by about 92% in liver and 89% in spleen (Figure 2A-B) and this protection was seen to persist till 56 days (8 weeks) of infection; 9662.6% and 9761.9% reduction in hepatic and splenic parasite burden respectively after 56 days of infection, compared with PBS-treated infected controls (Figure 2A-2B). Mice treated with SLA and control-CpG-ODNpulsed DCs (SLA-Ctrl-DCs) or only CpG-ODN pulsed DCs (CpG-DCs) showed a course of disease comparable to that in the PBS-treated infected group (Figure 2A-2B).
We observed significant reduction of parasitic burden (92% in liver and 89% in spleen; Figure 2A-B) in mice 5 wks post vaccination. To determine whether these SLA-CpG-DCs vaccinated BALB/c mice which resolved the primary infection were able to resist a second infection, they were re-infected with L. donovani intravenously at 5-week post-vaccination (4-week after initial infection) and were sacrificed at 12-week of initial infection and organ parasite burden was determined. Age-matched normal mice were also infected simultaneously and were considered as age-matched controls. We found that, mice vaccinated with SLA-CpG-DCs were more resistant to re-infection with L. donovani ( Figure 2C) compared to only CpG-DCs or SLA-Ctrl-DCs vaccinated mice.

Effect of CXCL10 Depletion on Protective Immunity Induced by the SLA-CpG-DCs
The role of CXCL10 was assessed in the induction of a protective immunity induced by the SLA-CpG-DCs. For this purpose, BALB/c mice were vaccinated by SLA-CpG-DCs, challenged 7 days thereafter by L.donovani, and analyzed for hepatic and splenic parasitic burden, in the absence of CXCL10. The depletion of CXCL10 by anti-CXCL10 mAbs for the duration of 4 weeks following infection (5 weeks following vaccination) abrogated the protective immunity induced by vaccination with SLA-CpG-DCs (Figure 3). The apparent lack of protection in CpG-DCs vaccinated sets, despite their high production of CXCL10 when stimulated with CpG-ODN in vitro, suggests that indirect signaling via TLR is not adequate to stimulate the expansion of host-protective Th1-response, signifying that the host-protective response was reliant on both CpG-ODN mediated DC stimulation and leishmanial antigen presentation by these DCs.

Vaccination with SLA-CpG-DCs Induces a CXCL10 Mediated CD8 + T Cell Response in Parasitized Mice
CXCL10 plays a critical role in the effector CD8 + T cell generation and was necessary for its trafficking [28]. CD8 + T cells are primarily responsible for protection during CpG-ODN stimulated DC vaccination, because CpG-ODN stimulation makes DCs switch their antigen processing pathway toward the MHC class I type than the class II type, resulting in a dramatic induction of IFN-c secreting CD8 + T cell responses [29]. These IFN-c secreting CD8 + T cells are cytotoxic T lymphocytes in nature [30]. Our results showed that there was a significant enhancement of splenic IFN-c + CD8 + T-cells when parasitized mice were vaccinated with SLA-CpG-DCs compared to infected or unprotected group of parasitized mice ( Figure 4A). To establish the role of CXCL10 in the induction of IFN-c + CD8 + T cells following SLA-CpG-DCs vaccination we depleted CXCL10 in vivo during the course of the vaccination. We observed significant reduction of IFN-c + CD8 + T cells in mice depleted of CXCL10 in contrast to non-depleted controls suggesting a direct role of CXCL10 in the generation of IFNc + CD8 + T cells in the SLA-CpG-DCs vaccinated mice.
To determine whether SLA-CpG-DCs vaccination generated CD8 + T cells can direct antigen-specific immune responses against L. donovani, antigen-specific proliferative responses were measured using purified CD8 + T cells from different groups of vaccinated mice. The CD8 + population purity was routinely confirmed to be around 98% ( Figure S1).Significantly higher proliferation of CD8 + was observed in the CD8 + cells isolated from the SLA-CpG-DCs vaccinated mice compared to CD8 + T cells from only infected, only CpG-DCs or SLA-control-ODN-DC vaccinated sets ( Figure 4B). In a separate study, we have evaluated CD8 + T-cell dependent production of IFN-c by stimulating purified CD8 + T cells from differently vaccinated groups, in vitro with SLA for 72 hours. Significantly higher IFN-c production was observed in CD8 + T cells isolated from SLA-CpG-DC treated parasitized mice, as compared to CD8 + T cells from only infected or unprotected group of mice ( Figure 4C). These data indicate that SLA-CpG-DCs have a significant impact on the generation of an antigen-specific CD8 + T cell response against the parasite.
To assess the contribution of CD8 + T cells in the induction of protective immunity after DC-mediated vaccination in vivo, BALB/c mice were transiently depleted of CD8 + T cells by intra-peritoneal injection of CD8 depleting antibody. While animals that had been immunized with SLA-CpG-DCs were fully protected against the infection, CD8 depleted SLA-CpG-DCs vaccinated mice showed almost no protection signifying the role of CD8 + T cells in the protective immunity following DC vaccination ( Figure 4D). Besides, SLA-CpG-DCs vaccinated mice receiving control antibody show full protection against Leishmania infection ( Figure 4D).

CXCL10 Depletion Reduces the Cytotoxicity of CD8 + T Cells in SLA-CpG-DCs Vaccinated Parasitized Mice
The cytotoxicity of CD8 + T cells is exerted through two processes: one is mediated through perforin, a membrane pore forming molecule, and granzyme B which activate caspase dependant apoptosis of the target cells [31]. Our data revealed Figure 6. CD8 + T cell cytotoxicity. CD8 + T cells purified from SLA-CpG-DCs vaccinated parasitized mice 28 days after infection were co-incubated with autologous uninfected (I) and Leishmania-infected macrophages (II) in a 10:1 ratio. In another set, CD8 + T cells were purified from CXCL10 depleted SLA-CpG-DCs vaccinated parasitized mice 28 days after infection and co-incubated with autologous Leishmaniainfected macrophages in a 10:1 ratio (III). After 4 h, the cells were harvested and stained with anti-CD14-FITC antibodies to select the target macrophages (A). The dot plots were derived from the gated events based on the region encircling positive cells. This CD14-FITC positive population were analyzed for propidium iodide staining to detect killed macrophages (B). M1: Apoptotic peak, M2: G1 peak, M3: S peak and M4: G2+M peak. The data was analyzed on a flow cytometer (FACS Calibur), using the Cell Quest program. These data were from one of three experiments conducted in the same way with similar results. The error bars represent mean 6 SD. **P,.001, compared to SLA-CpG-DC vaccinated parasitized mice. doi:10.1371/journal.pone.0048727.g006 that there was an increase in both perforin + and granzyme B + CD8 + T cells after SLA-CpG-DCs vaccination compared to infected group of mice ( Figure 5A-B). Depletion of CXCL10 reduces both perforin + and granzyme B + CD8 + T following SLA-CpG-DCs vaccination indicating a direct role of CXCL10 in perforin and granzyme B + activation in CD8 + T cells of the vaccinated mice. We also checked mRNA levels of both perforin and granzyme B in purified CD8 + T cells, which was also found to be increase in the SLA-CpG-DCs vaccinated parasitized mice compared to infected mice. The mRNAs of both perforin and granzyme B was also reduced following CXCL10 depletion ( Figure 5C-D). Therefore, these results indicated that CXCL10 contributed towards the generation of perforin + granzyme B + CD8 + T cells following SLA-CpG-DCs vaccination.
Antigen-primed CD8 + T cells have the capacity to destroy autologous infected macrophages [32]. To assess the cytotoxicity of these primed CD8 + T cells purified CD8 + T cells were isolated from SLA-CpG-DCs vaccinated and CXCL10 depleted SLA-CpG-DCs vaccinated L. donovani infected mice 28 days following infection and were co-cultured with autologous L.donovani-infected macrophages in a 10:1 ratio. After 4 h, the co-cultured cells were harvested and stained with anti-CD14-FITC antibodies to select the target macrophages. They were also analyzed by propidium iodide staining to detect the killed macrophages. Our data revealed that macrophages co-cultured with CD8 + T cells from SLA-CpG-DCs vaccinated mice showed a significant higher degree of killing than macrophages co-cultured with CD8 + T cells from CXCL10 depleted SLA-CpG-DCs vaccinated mice ( Figure 6). In addition, there is no effect of these primed CD8 + T cells from SLA-CpG-DCs vaccinated parasitized mice on uninfected macrophages signifying antigen-dependant specific killing ( Figure 6).

Humoral Responses in SLA-CpG-DCs Vaccinated L. donovani-infected Mice
To determine the type of humoral immune response induced by SLA-CpG-DCs against experimental VL, four weeks after the infection, IgG1 and IgG2a responses in the sera of different groups of mice was also determined. IgG1 is induced by IL-4, a Th2 cytokine, whereas IgG2a is induced by IFN-c, a Th1 cytokine [33]. Serum from SLA-CpG-DCs vaccinated mice mounted 10.3 fold higher SLA specific IgG2a compared to only infected mice, and almost 1.38 fold decrease of SLA specific IgG1 compared to only infected mice (Figure 7). This protected group of mice contained low levels of L.donovani-specific IgG1 and high levels of parasite-specific IgG2a Ab and exhibited the highest IgG2a/IgG1 ratio (2.38) (Figure 7). This indicates that vaccination with SLA-CpG-DCs induced a shift toward a Th1-dominated immune response after L. donovani infection.

Discussion
The induction of an effective cellular immune response against Leishmania pathogen requires a strong clonal expansion of antigenspecific CD8 + T-cells [34,35]. L. donovani evades this defence by restraining the effector functions of T-cell responses [36]. In this study, we demonstrated that DCs activated with a prominent TLR ligand CpG-ODN and pulsed with SLA induce a highly effective protective immunity against leishmanial pathogenesis via generation of antigen-specific CD8 + T-cells in vivo. Previously it was reported that the protection conferred by antigen pulsed CpG-ODN stimulated DCs is independent of DC-derived IL-12 [4]. But there is no clear understanding about the role played by CpG-ODN stimulated DCs in the generation of antigen-specific CD8 + T-cells in the context of leishmanial pathogenesis. Here, we demonstrate for the first time that the induction of a CD8 + T cell mediated anti-leishmanial protective immunity by SLA-CpG-DCs is entirely dependent on the production of CXC chemokine CXCL10 by these DCs. Our in vivo experiments demonstrated that SLA-CpG-DC vaccination could significantly restrict parasite growth in spleen and liver after 28 days of infection (Figure 2 A-B), and this protective immunity is abrogated with anti-CXCL10 mAbs resulting in the reappearance of high hepatic and splenic parasitic growth in the immunized animals ( Figure 3).
The type of DC stimulus is a critical factor which determines the capacity of DCs to direct a T cell response. TLR ligand stimulated DCs are capable of cross-presenting exogenous antigens to CD8 + T-cells by attracting naïve cytotoxic T lymphocytes though the generation of a chemokine gradient [37,38]. Our in vitro experiments showed that stimulation of DCs with CpG-ODNs resulted in the secretion of CXCL10, a CXCR3 ligand, which might attract naïve CTLs and increase the likelihood of the encounter of CTL's with licensed DCs for effective cross priming. The effector function of CD8 + T cells relies on CXCR3 signaling. CXCR3 signaling is critical for the chemotaxis, activation, and cytotoxic responses elicited by CD8 + T cells [25]. In agreement with the above findings, we observed an enhancement of IFN-c secreting leishmanial antigen specific CD8 + cytotoxic T lymphocytes upon SLA-CpG-DC vaccination ( Figure 4A). Depletion of CXCL10 efficiently inhibited both the generation and antigen dependant proliferation of these CD8 + cytotoxic T lymphocytes (Figure 4 A,  B).
Previous studies have demonstrated that CD8 + effector responses are required for protection against Leishmania infection [34,35,39,40]. Recently, it has been shown that, adoptive transfer of CD8 + T cells reduced L. donovani infection in susceptible BALB/ c mice by eliminating CD4 + CD25 + T regulatory cells via the induction of cytotoxic mediators, granzyme and perforin [41]. Our data revealed that there was a significant increase in granzyme and perforin secreting CD8 + T cells which most likely contributes to the protection conferred by the SLA-CpG-DCs (Figure 5 A, B) as depleting these CD8 + T cells results in significant increase in the liver and spleen parasitic burden ( Figure 4D).
CXCL10 plays a critical role in this activation of granzyme and perforin secreting CD8 + T cells because mice lacking CXCL10 receptor showed reduced CTL activity and impairment of perforin, and granzyme B expression by CD8 + T cells [25]. Neutralization of CXCL10 in the immunized group reduces both granzyme and perforin secreting CD8 + T cells as well as their cytotoxic properties ( Figure 5 A-B, 6). CXCL10, with known antileishmanial property can induce host-protective immune response by regulating the CD4 + CD25 + T regulatory cell functioning in Leishmania donovani-infected mice [22,42,43]. Thus the changes in effector CD8 + T cell functioning following SLA-CpG-DCs vaccination can be further correlated with T regulatory cell functioning during leishmanial pathogenesis as CXCL10 can regulate the balance between effector T cells and Treg cells. Therefore, these findings demonstrate a critical role of CXCL10 in effector T cell activation in this infectious disease model following SLA-CpG-DCs vaccination.
Collectively, these findings illustrate that upon SLA-CpG-ODN stimulation DC might function as a potent adjuvant for induction of an antigen-specific protective cellular immunity against leishmania-induced pathogenesis. The evidence presented here indicates that the anti-leishmanial protective immunity by SLA-CpG-DC vaccination is dependent on the activation CXC chemokine CXCL10. Inhibition of CXCL10 resulted in abrogation of the vaccination effect as indicated by a failure of CXCL10depleted mice to control a subsequent leishmanial challenge. The protection also requires the activation of effector CD8 + T cells. These findings were supported by a decrease in CD8 + T cell activation in CXCL10 -depleted mice associated with the reduction of perforin, granzyme B secretion and decrease in the number of Ag-specific CD8 + T cells producing IFN-c. Taken together, these data describe a novel role for CXCL10 in the generation of protective immunity induced by SLA-CpG-DC vaccination, representing an immune response that leads to disease control against experimental VL. Figure S1 Purity of CD8 + T cells. Spenocytes (1610 6 ) were isolated from spleen of differently vaccinated mice 28 days after infection. Splenocytes were then stained with CD8-FITC antibody and analyzed on a flow cytometer. The purity of these CD8 + T cells was found to be around 98%. (TIF)