Altered T-Cell Responses by the Periodontal Pathogen Porphyromonas gingivalis

Several studies support an association between the chronic inflammatory diseases periodontitis and atherosclerosis with a crucial role for the periodontal pathogen Porphyromonas gingivalis. However, the interplay between this pathogen and the adaptive immune system, including T-cells, is sparsely investigated. Here we used Jurkat T-cells to determine the effects of P. gingivalis on T-cell-mediated adaptive immune responses. We show that viable P. gingivalis targets IL-2 expression at the protein level. Initial cellular events, including ROS production and [Ca2+]i, were elevated in response to P. gingivalis, but AP-1 and NF-κB activity dropped below basal levels and T-cells were unable to sustain stable IL-2 accumulation. IL-2 was partially restored by Leupeptin, but not by Cathepsin B Inhibitor, indicating an involvement of Rgp proteinases in the suppression of IL-2 accumulation. This was further confirmed by purified Rgp that caused a dose-dependent decrease in IL-2 levels. These results provide new insights of how this periodontal pathogen evades the host adaptive immune system by inhibiting IL-2 accumulation and thus attenuating T-cell proliferation and cellular communication.


Introduction
Accumulating amount of data during recent years support an association between periodontitis and atherosclerosis, which both are inflammatory conditions involving different kinds of immune cells [1]. The role of T-cells in atherosclerosis is well established [2,3], however the involvement of T-cells in the pathogenesis and progression of periodontal disease is not fully elucidated [4]. T-cell activation and subsequent IL-2 secretion, which acts to further promote T-cell proliferation, play an important immune regulatory role. Disruption of IL-2 transcription and expression leads to T-cell anergy [5] and as a consequence this would result in an alteration of the antibody-based immunity by B-cells [6]. Andrukhov and colleagues [7] reported a significant decrease in serum IL-2 levels in patients suffering from periodontitis compared to healthy controls. Furthermore, regulatory T-cells and their release of the anti-inflammatory cytokine IL-10 have, in response to IL-2, been shown to exert anti-atherogenic effects [3,8,9]. Both CD4 + and CD8 + T-cells are present in atherosclerotic lesions [10], and may upon activation amplify the inflammatory condition in the atherosclerotic plaque through secretion of cytokines. Sasaki and colleagues [11] suggested the use of anti-CD3 to prevent atherosclerosis development and progression. They showed that administration of anti-CD3 activated regulatory T-cells and reduced atherosclerotic lesions and accumulation of other immune cells. Unravelling the effects of the periodontal pathogen Porphyromonas gingivalis on T-cells will contribute to the clarification of the mechanisms applied by this pathogen to evade host immune responses and cause disease. P. gingivalis is an anaerobic, gram-negative rod associated with periodontal disease progression including bone and tissue destruction [12]. Lamont and colleagues [13] showed that P. gingivalis could invade and translocate into the cytosol within gingival epithelial cells, demonstrating a possible mechanism for its establishment, replication and subsequent pathogenesis by evading the host immune system. Similar results were observed in heart and aortic endothelial cells [14], indicating an association between P. gingivalis-dependent periodontitis and cardiovascular disease. Another mechanism used by P. gingivalis to evade the immune system is through its ability to inhibit CXCL-8 expression [15], and as a consequence impair immune cell recruitment.
Several factors contribute to the pathogenesis of P. gingivalis, including LPS and cysteine proteinases. Establishment and growth of P. gingivalis has been associated with its production and secretion of proteinases. These enzymes are divided into arginine-specific (Rgp) and lysine-specific (Kgp) gingipains [16]. RgpA-Kgp complexes have been reported to inactivate the T-lymphocytederived cytokines IL-4 and IL-5 [17] that are important for the activation and proliferation of B-lymphocytes. Even though cytokines and chemokines are expressed in response to P. gingivalis, their release and subsequent action on leukocyte migration is thus modulated due to the enzymatic activity of proteinases that cleave and inhibit the biological properties of different cytokines, including CXCL-8 [18] and TNF [19]. It is therefore important to determine the interactions between P. gingivalis and different host immune cells, as well as possible alterations in inflammatory gene regulation.
It is important to analyse T-cell responses to P. gingivalis infection, since this periodontal pathogen has been shown to be translocated with T-cells in atherosclerotic plaques. We hypothesize that P. gingivalis is able to suppress T-cell-derived responses, which benefits the pathogen to establish itself and proliferate. The aim of the present study was to characterize the effects of P. gingivalis on T-cell-mediated inflammatory responses and gene regulation.
Heat-killed P. gingivalis and heat-inactivated P. gingivalis supernatants were prepared following incubation at 70uC for 1 h. To ensure that the bacteria were killed, 10 ml of the heat-killed suspension was spread on a fastidious anaerobe agar plate and incubated at 37uC for 5 days. The absence of colony formation was used as an indicator that no viable bacteria were present in the suspension. P. gingivalis supernatants were sterile filtered through a 0.2 mm filter before being used. Both P. gingivalis and its supernatant were used fresh for every experiment.
Two selected inhibitors of cysteine proteinases (Leupeptin, Roche Diagnostics Corporation, USA and Cathepsin B Inhibitor II, Calbiochem, Germany) were used to determine the role of Argand Lys-gingipain activities. Viable P. gingivalis were incubated with different concentrations of the inhibitors for 1 h prior to stimulation of Jurkat T-cells. To further assess the contribution of gingipains, purified Arg-gingipain B (RgpB, Athens GA, USA) was used.
E. coli MG1655 were grown on Luria-Bertani (LB) plates and incubated at 37uC overnight. Single colony was inoculated into 10 ml LB and the tube was incubated at 37uC overnight on shaker set at 200 rpm. The bacteria were then harvested for 10 min at 30006g, washed with 3 ml KRG and re-suspended in KRG.

Isolation of primary cells
PBMC were isolated by the density gradient medium Ficoll-Paque TM Plus (Amersham Biosciences, Sweden) according to the manufacturers' instructions. Briefly, freshly collected blood from healthy donors was diluted with an equal volume of PBS, and 4 ml were carefully layered on top of 3 ml Ficoll-Paque Plus. The tubes were centrifuged at room temperature for 30 min at 3006g. PBMC were recovered from the interface and washed twice with PBS to remove excess Ficoll-Paque Plus and platelets. The cells were suspended in RPMI media supplemented with 10% foetal bovine serum and incubated in a stable environment at 95% air, 5% CO 2 and 37uC for 2 days. Suspended cells were recovered, washed and cultured in a separate T75 flask for 24 h. The cells were then used to determine IL-2 expression in response to P. gingivalis.

Enzyme-linked immunosorbent assay (ELISA)
ELISA was performed on supernatants from challenged Jurkat T-cells to quantify IL-2 (BD OptEIA Set Human IL-2, BD Biosciences, USA) according to the manufacturer's instructions. Briefly, Jurkat T-cells were either pre-treated with P. gingivalis or bacterial supernatant for 1 h followed by stimulation with 50 ng/ ml PMA and 1 mg/ml Calcium Ionophore (Calcium Ionophore A23187 mixed calcium magnesium salt, Sigma #C5149, USA) or stimulated with PMA and Calcium Ionophore prior to treatment with P. gingivalis or bacterial supernatant. The cells were thereafter centrifuged at 956g for 5 min and the supernatants were collected and stored at 280uC until use.

Transfection and luciferase measurement
Activator protein (AP)-1 and nuclear factor (NF)-kB activity were measured by using luciferase reporter plasmids. Briefly, reporter plasmid (pAP1-Luc, NF-kB-Luc), internal control plasmid (Renilla) (Promega, USA) and lipofectamine 2000 (Invitrogen, USA) were added to each well at 0.54 mg/well, 0.06 mg/well and 1.5 ml/well, respectively. Initially, reporter plasmid and Renilla were mixed separately with OptiMEM (Gibco, USA). After 5 min of incubation at room temperature, lipofectamine 2000 was added and the mixture was incubated further for 20 min at room temperature. The transfection was allowed to proceed overnight at 37uC, after which, the cells were centrifuged, the media removed and fresh pre-warmed media added. The cells were lysed and luciferase activity was measured using the Dual-LuciferaseH reporter assay system (Promega, USA) according to the manufacturer's instructions on a TD 20/20 luminometer (Turner Designs, Sunnyvale, CA).

Measurement of ROS production
ROS production in Jurkat T-cells was analyzed using a lumiaggregometer (Chrono-Log Corp., Havertown, PA). Briefly, Jurkat T-cells (10 6 cells/ml) were suspended in complete RPMI 1640 media supplemented with 10% FBS containing 50 mM luminol and 4 U/ml HRP. The cells were incubated at 37uC for 15 min, at 800 rpm, before being stimulated with 10 8 CFU/ml P. gingivalis for 30 min, during which time chemiluminescence was registered.

Measurement of [Ca 2+ ] i
Cytosolic Ca 2+ concentration was measured by using the fluorescent indicator Fura-2. Briefly, Jurkat T-cells were washed twice and resuspended in KRG to yield a cell-density of 10 6 cells/ ml. The cells were loaded with 4 mM Fura-2-acetoxymethylester (AM) for 40 min during gentle agitation. The cells were then washed twice. The extracellular Ca 2+ concentration was set to 1 mM by addition of CaCl 2 and intracellular Ca 2+ concentration was determined in 2 ml aliquots at 37uC, 300 rpm using a Hitachi F2000 spectrofluorometer (Hitachi Ltd. Tokyo, Japan). Fluorescence emission and excitation was registered at 510 nm and 340/ 380 nm, respectively. Maximal and minimal ratios were determined following addition of 0.1% Triton X-100 and 24 mM EGTA. The change in intracellular Ca 2+ concentration was calculated by using the equation described by Grynkiewicz and colleagues [20]. Calcium Ionophore was used as a positive control.

Reverse transcription quantitative PCR (RT-qPCR)
RT-qPCR was used to determine gene expression levels of il-2 in response to viable and heat-killed P. gingivalis. Briefly, Jurkat Tcells were pre-treated with P. gingivalis for 1 h, followed by stimulation with PMA/Ionophore for 24 h. RNA was extracted using RNeasyH Plus Micro Kit (Qiagen, USA) according to the manufacturer's recommendations. Reverse transcription was performed using MaximaH First Strand cDNA Synthesis Kit (Fermentas, Sweden). The following primer sequences were used: forward-ACCTCAACTCCTGCCACAATGTAC, reverse-TCAGTTCTGTGGCCTTCTTGGGCA. Thermal cycling conditions for SYBR Green (Fermentas) consisted of a denaturation step at 95uC for 10 min followed by 40 cycles of 95uC for 15 s and 60uC for 60 s. Gene expression was analyzed using a 7900 HT real-time PCR instrument (Applied Biosystems). The obtained Ct values were normalized against GAPDH. Relative quantification of gene-expression was determined by using the DDCt method. The DCt was calculated by subtracting the Ct of GAPDH from the Ct of il-2 for each sample. The DDCt was calculated by subtracting the DCt of the control sample from the DCt of each treated sample. Fold change was generated by using the equation 2 2DDCt .

Fluorescence microscopy
Jurkat T-cells were stimulated with FITC-labeled P. gingivalis (10 8 CFU/ml, MOI:100) for 24 h and fixation overnight in 4% paraformaldehyde (PFA) at 4uC. F-actin was visualized by incubating the cells with 2 units Alexa FluorH 594 phalloidin (Invitrogen) and 100 mg/ml lysophosphatidylcholine in darkness for 1 h at room temperature. The cells were washed and mounted on a coverslip. Adherence of P. gingivalis to Jurkat T-cells was analyzed by confocal microscopy (Leica Microsystems, Heidelberg, Germany).

P. gingivalis triggers [Ca 2+ ] i changes
Changes in intracellular free calcium concentration [Ca 2+ ] i is an important action for the establishment of a proper response to foreign pathogens. This prompted us to determine the levels of [Ca 2+ ] i in Jurkat T-cells in response to viable-and heat-killed P. gingivalis. The basal [Ca 2+ ] i levels of unstimulated cells was around 45614 nM. Viable P. gingivalis increased [Ca 2+ ] i by 5.1, 4.5 and 8.3 fold in response to 10 7 , 5610 7 and 10 8 CFU/ml, respectively (figure 1a). However, T-cells did not respond when P. gingivalis were heat-killed (HK, figure 1a). Representative traces of the calcium changes following treatment of Jurkat T-cells with viable E. coli MG1655, viable P. gingivalis and heat-killed P. gingivalis are shown in figure 1b. As a positive control we used the calcium ionophore ionomycin, which caused a significant increase in [Ca 2+ ] i by ,78 fold (data not shown). Viable E. coli MG1655 (5610 7 CFU/ml) was used as a control, and resulted in minor changes in [Ca 2+ ] i .

P. gingivalis binds to T-cells and induces ROS production
The ability of P. gingivalis to attach to Jurkat T-cells and cause cell aggregation was shown by labeling the bacteria with FITC and stain the actin cytoskeleton with Alexa Fluor 594 phalloidin (figure 2a). We found that P. gingivalis efficiently binds to Jurkat Tcells and stimulate morphological changes and aggregate formation. As a consequence, bactericidal molecules, such as reactive oxygen species (ROS) may be released of P. gingivalis-T-cells interaction. ROS may function either, at low concentrations, as signaling molecules derived from different metabolic processes or, at high concentrations, as bactericidal toxic molecules produced by NADPH-oxidases. By using luminol-dependent chemiluminescence, changes in ROS production over time was analyzed in Jurkat T-cells following exposure to P. gingivalis. The cells where either left untreated, or exposed to viable P. gingivalis for 30 min. After a lag phase of around 12-14 min, viable P. gingivalis caused an extensive and long-lasting ROS production reaching maximum after 20 min (figure 2b).

Viable P. gingivalis inhibits IL-2 expression and accumulation
Alteration in the levels of intracellular calcium and ROS prompted us to determine AP-1 activity in response to P. gingivalis.
Jurkat T-cells were transfected with luciferase-reporter plasmids containing cis-acting elements for AP-1 or NF-kB, followed by exposure to viable or heat-killed P. gingivalis. Viable, but not heatkilled, P. gingivalis caused a significant inhibition of AP-1 activity (figure 3a), while both viable and heat-killed P. gingivalis reduced basal level of NF-kB activity (figure 3b). Considering the apparent effects on Jurkat T-cell signaling involving calcium, ROS and the transcription factors AP-1 and NF-kB, we then determined whether P. gingivalis and its supernatant affected accumulation of IL-2. The PMA/Ionophore-induced IL-2 accumulation after 24 h was significantly decreased by pre-treatment with viable bacteria, while exposure to heat-killed bacteria resulted in increased IL-2 levels, compared to the positive control (figure 4a). Pre-exposure of Jurkat T-cells to either untreated or heat-treated P. gingivalis supernatant resulted in a significant reduction in IL-2 levels. A viability assay showed that the observed inhibition of IL-2 accumulation by viable P. gingivalis was not due to cell death. Cell-density increased by 20% after stimulation of Jurkat T-cells with viable P. gingivalis for 24 h, compared to 50% increase in the control and cells treated with heat-killed P. gingivalis (data not shown). Furthermore, treatment of Jurkat T-cells with either viable or heat-killed P. gingivalis, without PMA/Ionophore, did not alter IL-2 levels compared to the untreated negative control (data not shown). Inhibition of IL-2 accumulation by bacterial supernatants was shown to be due to small molecular weight compounds present in the fastidious anaerobe broth, as determined by molecular mass fractionation, rather than any bacterial-derived compound(s) (data not shown).
Furthermore, pre-incubation of Jurkat T-cells with different concentrations of viable P. gingivalis showed that inhibition of IL-2 accumulation was dose-dependent (figure 4b). A final concentration of 5610 6 CFU/ml (MOI:5) of viable bacteria was sufficient to significantly reduce IL-2 accumulation by ,2.4 fold, compared to the positive control PMA/Ionophore, while the highest concentration 10 8 CFU/ml reduced IL-2 accumulation by ,14 fold. We thereafter aimed to confirm these results by using primary cells isolated from healthy volunteers. Viable, but not heat-killed P. gingivalis caused a significant reduction in IL-2 accumulation (figure 4c).

Viable P. gingivalis targets IL-2 at the protein level
In the experiments so far, the cells have been pre-exposed with either viable or heat-killed P. gingivalis to determine whether this treatment can alter intracellular mechanisms involved in IL-2 transcription and expression. In order to determine whether viable P. gingivalis affect produced and accumulated IL-2, Jurkat T-cells were first stimulated with PMA/Ionophore for 24 h, and then exposed to viable P. gingivalis for the indicated times (figure 5). IL-2 levels remained constant over time (0.5-24 h) in the untreated and the PMA/Ionophore-stimulated groups, while treatment with viable P. gingivalis resulted in a significant reduction in IL-2 levels already after 30 min and the levels continued to decrease over time (figure 5a). A second set of experiment was performed to determine whether the bacteria can alter the expression and subsequent release of IL-2. Jurkat T-cells were stimulated for IL-2 production as mentioned above, followed by centrifugation to exclude the cells. P. gingivalis was then added to the cell-free, IL-2 containing media for the indicated times (figure 5b). A similar trend was observed where P. gingivalis caused a significant reduction in IL-2 levels.
We then aimed to determine whether the observed inhibition of the extracellular IL-2 accumulation was affected at the transcript level. Jurkat T-cells were pre-treated with viable-or heat-killed P. gingivalis, followed by induction with PMA/Ionophore. RT-qPCR analysis showed that il-2 mRNA levels, induced by PMA/ Ionophore, remained elevated in response to both viable-and heat-killed P. gingivalis treatment ( figure 6).

Degradation of IL-2 by arginine gingipains
The ability of viable P. gingivalis to cleave or in-activate the preaccumulated IL-2 led us hypothesize that P. gingivalis-derived proteinases are involved in the inhibition of IL-2 accumulation. Analysis of the IL-2 amino acid sequence revealed 5 arginine cleavage sites and 11 lysine cleavage sites (figure 7a). In order to determine the involvement of these specific proteinases, viable bacteria were incubated with the selected arginine-specific and lysine-specific proteinase inhibitors Leupeptin and Cathepsin B inhibitor II, respectively, prior to exposure of Jurkat T-cells. The results showed that inhibition of IL-2 accumulation was partially dependent on the action of Rgp proteinases, either released and free in suspension or bound to the bacteria (figure 7b). Furthermore, treatment of PMA/Ionophore stimulated Jurkat T-cells with purified RgpB resulted in a dose-dependent inhibition of IL-2 accumulation (figure 7c).

Discussion
T-cells are found in atherosclerotic plaques and their contribution to the progression of this inflammatory condition is well established [2]. Proliferation of effector T-cells in plaques requires IL-2, and the activity of effector T-cells is strictly controlled by regulatory T-cells [3]. Intracellular free Ca 2+ is an important signaling molecule that is required for the activation of T-cells upon recognition of a foreign antigen [21]. Activation of the transcription factors AP-1, NFAT and NF-kB, and subsequent gene-expression and cytokine release requires a sustained Ca 2+ influx [22]. These transcription factors are activated through  Ca 2+ -dependent signaling proteins, including PKC [23] and calmodulin/calcineurin complex [24]. We found that intracellular Ca 2+ levels increased in response to viable, but not heat-killed, P. gingivalis. The fact that only viable bacteria were able to elevate [Ca 2+ ] i may be due to that they possess intact fimbriae, which has been reported as an important feature, enabling the bacteria to attach, invade and induce an inflammatory response [25]. Furthermore, heat-sensitive bacterial proteinases have been reported to cleave and thus activate proteinase-activated receptor-2 (PAR-2), which is expressed on T-cells [26], leading to an increase in [Ca 2+ ] i [27].
Intracellular ROS production is important for elimination of invading pathogens and has been reported to influence T-cell activation [28]. In this study, we found that P. gingivalis binds to and aggregate T-cells and induces an extensive ROS production, which may reflect an ability of this pathogen to recognize specific cell surface receptors and induce intracellular signaling cascades involving ROS. P. gingivalis has previously been shown to affect the activity and/or expression of several cell surface receptors that are expressed on a variety of cells, including T-cells. These receptors include proteinase-activated receptors that are activated by P. gingivalisderived proteinases [29]. However, microarray analysis showed an inhibitory effect of TCR expression by this periodontal pathogen [4]. Furthermore, Kitamura and colleagues reported that P. gingivalis was able to cleave CD4 and CD8 and thus impair T-cell activation [30]. P. gingivalis probably utilizes such a strategy . IL-2 accumulation decreases in response to viable P. gingivalis and its derived supernatant. A-Jurkat T-cells (10 6 cells/ml) were pre-treated with 10 8 CFU/ml of viable or heat-killed (HK) P. gingivalis (MOI:100) as well as 10% untreated-or heat-treated (HT) supernatant from P. gingivalis broth cultures for 1 h. The cells were then stimulated with 50 ng/ml PMA and 1 mg/ml Calcium ionophore for 24 h. IL-2 accumulation was significantly reduced by viable, but not heat-killed P. gingivalis in Jurkat T-cells, while both untreated and heat-treated bacterial supernatant resulted in a significant IL-2 reduction. B-T-cells were pre-treated with the indicated concentrations of viable P. gingivalis (MOI:0.5, 1, 5, 10, 50 and 100, respectively) for 1 h followed by stimulation with 50 ng/ml PMA and 1 mg/ml Calcium ionophore for 24 h. IL-2 accumulation was reduced in a dosedependent manner. C-Primary cells were isolated as described in materials and methods. Cells were pre-treated with viable or heat-killed P. gingivalis for 1 h, followed by stimulation with 50 ng/ml PMA and 1 mg/ml Calcium ionophore for 24 h. Viable, but not heat-killed P. gingivalis (MOI:100) resulted in a significant reduction in IL-2 accumulation. *-p,0.05; **-p,0.01; ***-p,0.001 (Statistical significance between different treatments and the positive control PMA/Iono, Student's t-test). doi:10.1371/journal.pone.0045192.g004 to evade the host immune system, which benefits its establishment and proliferation. Several studies have reported the ability of P. gingivalis to invade host cells, including gingival epithelial cells [13] and heart endothelial cells [14] and that expression of fimbriae enables pathogen attachment and invasion [31]. Other strategies utilized by this pathogen to evade the host immune system are by affecting cells of the innate immune system. These effects are mainly due to the action P. gingivalis-derived proteinases and include proteolysis of CD14 on monocytes [32] and C5a receptor on neutrophils [33]. This would, as a consequence, lead to reduced bacterial recognition by monocytes and neutrophil migration, respectively.
Furthermore, viable P. gingivalis reduced AP-1 and NF-kB activity below basal levels. Transcriptional regulators, including AP-1 and NF-kB, are important for inflammatory gene-expression, such as CXCL-8 and IL-6 [34]. In addition, AP-1 has been shown to be an important regulator of IL-2 expression, in cooperation with NFAT [35], through PKC [36]. Mutation of the NF-kB site did not affect IL-2 expression, while mutation of the AP-1 site or PKC depletion almost revoked IL-2 release. These observations indicate that the MAPK pathway and the transcription factor AP-1 play an important role in the induction of inflammatory responses in Jurkat T-cells. We therefore aimed to determine IL-2 expression in response to P. gingivalis. Presence of viable P. gingivalis significantly inhibited PMA/Ionophore-induced IL-2 accumulation in Jurkat T-cells suspension. This effect may be due to the action of P. gingivalis-derived proteinases that have been reported to regulate several cytokines and chemokines. Kobayashi-Sakamoto and colleagues [18] reported the involvement of proteinases in degradation of CXCL-8 and MCP-1 and the Th2 cytokine IL-4 and IL-5 have also been shown to be targets for degradation by P. gingivalis proteinases [17]. However, receptor activator of NF-kB ligand (RANKL) is induced in Jurkat T-cells by P. gingivalis secreted compounds [37], while P. gingivalis outer membrane proteins were shown to induce IL-17 rather than RANKL [38]. These observations indicate that different P. gingivalis-derived components can differentially regulate cytokine expression.
We thereafter aimed to determine whether addition of P. gingivalis could alter a pre-stimulated accumulation of IL-2 and observed that only viable bacteria were able to reduce IL-2 levels and did that in a time-dependent manner. IL-2 was shown to be targeted at the protein level, since the transcript levels were not affected by P. gingivalis. This indicates that P. gingivalis regulates Tcell activation and proliferation by targeting the accumulation of IL-2, considering the importance of this cytokine in P. gingivalismediated T-cell proliferation [39].
P. gingivalis-derived proteinases have been reported to inhibit cytokine and chemokine expression [40]. This inhibitory effect is dependent on the enzymatic activity of these proteinases that cleave and inactivate several inflammatory markers, including TNF [19] and IL-6 [41]. Yun and colleagues [42] showed that P. gingivalis-derived proteinases activated T-cells through proteaseactivated receptors, but were also able to degrade CD27, which is a TNF receptor family member, as well as its ligand CD70, present on B-cells. These observations indicate that cellular communication is interrupted by P. gingivalis and may result in an impaired host immune response and less efficient clearance of an infection. The involvement of proteinases in the inhibition of Figure 5. Viable P. gingivalis cleaves and prevents IL-2 accumulation. A-Jurkat T-cells (10 6 cells/ml) were stimulated with 50 ng/ml PMA and 1 mg/ml Calcium ionophore for 24 h followed by exposure to viable P. gingivalis (Viable Pg, 10 8 CFU/ml, MOI:100) for the indicated times. IL-2 accumulation was significantly decreased by P. gingivalis over time. B-Jurkat T-cells (10 6 cells/ml) were stimulated with 50 ng/ml PMA and 1 mg/ml Calcium ionophore for 24 h. The cells were then removed and viable P. gingivalis (Viable Pg, 10 8 CFU/ml, MOI:100) were added to cell-culture supernatants, containing secreted IL-2, for the indicated times. P. gingivalis is involved in cleaving and de-activating IL-2 proteins. The letters indicate significant differences compared to their respective positive control PMA/Iono at each specific time point. a-p,0.05; c-p,0.001 (Student's t-test). doi:10.1371/journal.pone.0045192.g005 Figure 6. RT-qPCR analysis of il-2 gene-expression in response to P. gingivalis. Jurkat T-cells (10 6 cells/ml) were pre-treated with 10 8 CFU/ml viable-or heat-killed (HK) P. gingivalis (MOI:100) for 1 h followed by stimulation with 50 ng/ml PMA and 1 mg/ml Calcium ionophore for 24 h. Il-2 mRNA levels were not affected by viable or heat-killed P. gingivalis. ***-p,0.001 (Statistical significance between different treatments and the positive control PMA/Iono, Student's t-test). doi:10.1371/journal.pone.0045192.g006 IL-2 accumulation in response to viable P. gingivalis, was shown by the partial restoration of IL-2 in the presence of Leupeptin. Furthermore, purified gingipains completely antagonized IL-2 accumulation. By considering the IL-2 amino acid sequence, the number of arginine and lysine cleavage sites were predicted and corresponded to 5 and 11, respectively. However, IL-2 was shown to be targeted by Rgp rather than Kgp proteinases. This is in accordance with the observations made by Yun and colleagues [42] showing that T-cell activation and subsequent cellular communication are interrupted by arginine-specific cysteine proteinases.
The importance of sustained IL-2 levels for growth of regulatory T-cells has previously been reported [43]. The effects of P. gingivalis and its proteinases on T-cells are evident. We show that IL-2 accumulation is targeted by P. gingivalis at the protein level, and partially through suppression of AP-1, unraveling a mechanism applied by P. gingivalis to benefit its establishment by altering adaptive immune responses. Hence, alteration of IL-2 levels benefits bacterial establishment and may also contribute to progression of the inflammatory state in atherosclerosis, considering that IL-2 play an important role in the clonal expansion of regulatory T-cells. Furthermore, effector T-cells are found in atherosclerotic plaques and are considered to contribute to the progression of the inflammatory process. Identification of immune-regulatory compounds from P. gingivalis and the effects of these compounds on different T-cell subsets may be crucial in the development of new strategies to restrict further progression of atherosclerotic plaque formation and development. Jurkat T-cells (10 6 cells/ml) were pretreated with 10 8 CFU/ml of viable P. gingivalis (MOI:100) for 1 h followed by stimulated with 50 ng/ml PMA and 1 mg/ml Calcium ionophore for 24 h. IL-2 accumulation was partially restored by Leupeptin, but not by Cathepsin B inhibitor. The asterisks indicate significant differences compared to their respective control (Viable Pg, without Leupeptin). C-Purified RgpB resulted in a dose-dependent inhibition of IL-2 accumulation. The asterisks indicate significant differences compared to the positive control PMA/Iono. *-p,0.05; **-p,0.01 (Student's t-test). doi:10.1371/journal.pone.0045192.g007