Absorption of Radionuclides from the Fukushima Nuclear Accident by a Novel Algal Strain

Large quantities of radionuclides have leaked from the Fukushima Daiichi Nuclear Power Plant into the surrounding environment. Effective prevention of health hazards resulting from radiation exposure will require the development of efficient and economical methods for decontaminating radioactive wastewater and aquatic ecosystems. Here we describe the accumulation of water-soluble radionuclides released by nuclear reactors by a novel strain of alga. The newly discovered green microalgae, Parachlorella sp. binos (Binos) has a thick alginate-containing extracellular matrix and abundant chloroplasts. When this strain was cultured with radioiodine, a light-dependent uptake of radioiodine was observed. In dark conditions, radioiodine uptake was induced by addition of hydrogen superoxide. High-resolution secondary ion mass spectrometry (SIMS) showed a localization of accumulated iodine in the cytosol. This alga also exhibited highly efficient incorporation of the radioactive isotopes strontium and cesium in a light-independent manner. SIMS analysis showed that strontium was distributed in the extracellular matrix of Binos. Finally we also showed the ability of this strain to accumulate radioactive nuclides from water and soil samples collected from a heavily contaminated area in Fukushima. Our results demonstrate that Binos could be applied to the decontamination of iodine, strontium and cesium radioisotopes, which are most commonly encountered after nuclear reactor accidents.


Introduction
Since the accidents at the Fukushima Daiichi Nuclear Power Plant on 11 March 2011, decontamination of the enormous quantities of water used to cool the reactors so that they can be shut down has become a critical issue. Indeed, it has been estimated that approximately 250,000 tones of water will be required by mid-January 2012 to cool down the reactors [1], injection of water at the rate of around half-a-million litres a day is required to keep the reactors at could shutdown [2]. In addition, the cost of the decontamination system developed by Areva (Paris, France) and Kurion (CA, US) may be as high as US $660 million, and the 2,000 cubic meters of the resulting radioactive sludge that will be generated will pose new long-term storage challenges by January, 2012 [1].
At the time of the Chernobyl nuclear accident, the plume that escaped from the reactor contaminated extensive areas as it was transported by the prevailing winds, not only in the vicinity of the reactor, but areas across the northern hemisphere [3,4]. At the time, water-soluble radioactive nuclides, such as the radioisotopes of cesium (Cs), iodine (I), and strontium (Sr), contaminated aquatic ecosystems and food products [5]. Despite the relatively short halflife of radioiodine, the incidence of thyroid cancer in children living in radioiodine-contaminated areas increased significantly [3]. Subsequent phases of environmental contamination were primarily due to 137 Cs, 134 Cs, and 90 Sr, which was considered harmful as Cs and Sr are concentrated in human muscle and bone, respectively [3]. At the Fukushima Daiichi Nuclear Power Plant, 18 PBq of 134 Cs, 15 PBq of 137 Cs, 2.0 PBq of 89 Sr, 0.14 PBq of 90 Sr, and 160 PBq of 131 I have been released into the environment to date [6], and radionuclides from the reactors in Fukushima have already been detected in Russia and Greece [7]. Thus, developing an effective and economical method for removing these radionuclides from radioactive wastewater and aquatic ecosystems has become an increasingly important issue.
The Fukushima nuclear accident was triggered by an act of God in the form a megathrust earthquake and associated tsunami. Since the natural environment exhibits an innate tendency towards recovery [8], we attempted to identify at least one of these recovery mechanisms that could be applied to the decontamination of radionuclides from the environment, rather than investigating artificial methods of decontamination. In this report, we show that a newly discovered green microalga efficiently absorb radioactive isotopes of iodine, strontium, and cesium.

Results
On the morning after a recent typhoon, we found floc of green algae in activated sludge at a wastewater treatment plant in Kitaibaraki, Ibaraki, Japan, approximately 78 km from the Fukushima Daiichi Nuclear Power Plant [9]. We isolated a strain of green microalgae that was viable at high temperatures up to 60uC and was resistant to acidic and alkaline conditions at a pH range of 3-11 (data not shown). This alga was also capable of growing in fresh water and seawater. This strain was unique in that it had abundant chloroplasts ( Figure 1A) and an extracellular matrix that was observed by India ink staining under a light microscope (Figure 1b). To clarify the taxonomic affiliation of this strain within the phylum Chlorophyta, we sequenced part of the 18S rRNA and an actin gene as described previously [10]. Phylogenetic analysis of the 18S rRNA gene sequences showed that the novel algal strain was embedded within a clade containing the members of the class Trebouxiophyceae and shared a node with Parachlorella kessleri (Figure 1c). However, amino acid sequences encoded by the actin mRNA revealed that the newly isolated algal strain appeared to be less closely related and P. kessleri (Figure 1d). Taken together, these findings suggested that the novel algal strain could be assigned to the genus Parachlorella, and we designated the strain Parachlorella sp. binos (Binos).
Transmission electron microscopy revealed that most of the cytosol of the alga was occupied by chloroplasts (Figure 1e), whereas the chloroplasts in a Chlorella sp. that is considered to be closely related to Binos, are restricted to the margins of the cytosol [11]. The external surface of the novel alga was also covered by a thick extracellular matrix (Figure 1e), which is not found in the Chlorella sp. [11]. Fourier transform infrared spectroscopy revealed that the extracellular matrix mainly consisted of alginate, an anionic polysaccharide commonly found in the cell walls of brown algae, but not in green algae [8] (Figure 1f).
When cultured under illumination (2000 lux), absorption of radioiodine by the alga was observed two hours after the addition of Na 125 I, and maximum uptake of 125 I was observed after 24 to 48 h (Figure 2a). This ability of Binos to adsorb iodine was found to be light-dependent as algal cells cultured in the dark did not take up radioiodine. In addition, 125 I uptake was also correlated with the density of algal cells ( Figure 2b); 100 mg/ml of cells in 1 ml of H 2 O with Na 125 I accumulated 38.763.1% of total radioiodine after 24 h. The bound/free ratio of 125 I calculated with the formula described in Materials and Methods reached 19.360.5 when 10 mg/ml of wet Binos added to the reaction (Figure 2c). Dehydration of Binos increased the bound/free ratio of 125 I up to 386662. A radioiodine binding study using nonradioactive iodine as a competitor revealed that the dissociation constant (K d ) and the maximal binding capacity (B max ) of iodine to algal cells were 141 mM (95% confidence interval (CI); 104-191 mM) and 2.52 nmol/mg wet weight (CI: 2.33-2.71 nmol/mg wet weight; 50.4 nmol/mg dry weight), respectively.
The process of photosynthesis is known to produce superoxides, such as hydrogen peroxide [12], and haloperoxidases, both of which mediate halide oxidation by hydrogen peroxide and have been implicated in iodine uptake by marine brown algae such as Laminaria sp [13,14]. We found that the addition of 10 mM of H 2 O 2 stimulated radioiodine uptake in dark conditions in a timedependent manner (Figure 2e). This finding suggested that iodine uptake by Binos was, at least in part, mediated by the increase in the oxidative power associated with photosynthesis.
In order to analyze the distribution of accumulated iodine ions at the subcellular scale, high-resolution secondary ion mass spectrometry (SIMS) was performed. Distribution of stable isotopes of carbon and nitrogen, 12 C and 14 N, localized the algal cells and those extracellular matrix (Figure 2f). After 24h incubation with 1 mM of 127 I under the light, accumulated iodine was mainly localized in cytosol (Figure 2g, h). This result clearly indicated that radioiodine ions are also transported across the cell wall and membrane.
The alga also exhibited an ability to bind 85 Sr, a c-ray emitting isotope, in a density-dependent manner; for example, at densities of 100 mg/ml, the alga accumulated 75.964.2% of the total 85 Sr added (Figure 3a). For 100 mg of wet alga, the bound/free ratio of 85 Sr was 31.966.7 (Figure 3b), and that in dried alga was 6386134. Binding of 85 Sr, which was observed after 1 min and reached maximal levels after 10 min, occurred independently of exposure to light (data not shown). An 85 Sr-binding study using nonradioactive Sr as a competitor revealed that the K d and B max associated with Sr binding to algal cells were 387 mM (CI: 318-472 mM) and 15.5 nmol/mg (CI:14.9-16.1 nmol/mg; wet weight), respectively ( Figure 3c). When the alga was dehydrated, B max was 310 nmol/mg (CI: 298-322). Competition analysis showed that the Ki associated with the binding of Ca to the alga was 183 mM (141-238 mM), which was significantly lower than the K d observed with Sr, even though both elements are divalent ions. Alginate in the extracellular matrix is an anionic polysaccharide with carboxyl groups that electrostatically cross-link with divalent cations, such as Ca or Sr. It may thus be possible to exploit the thick extracellular matrix that is characteristic of the alga to accumulate radioactive cations. SIMS mapping of the nonradioactive isotope of strontium clearly showed localized distribution of Sr at the outer surface of extracellular matrix (Figure 3d-f), while only a little of Sr were detected in the cytosol.
We then analyzed the capacity of the alga to adsorb and accumulate radioactive Cs, which has prompted extensive concern in Japan due to reports of food and soil contamination in recent weeks. The alga accumulated up to 41.064.8% of the 137 Cs 10 min after addition ( Figure 4a). When 100 mg/ml of Binos was added, the bound/free ratio of 137 Cs was 8.0061.49 (wet weight) and 160630 (dry weight) ( Figure 4b). Binding of 137 Cs to the alga was observed after 1 min, with maximal levels of binding observed after 10 min in the absence of illumination (data not shown). Competition analysis using nonradioactive Cs revealed that Cs bound to two sites on the alga with different affinities; K d values for the high and low affinity binding sites were 19.90 mM (CI: 6.20-64.2 mM) and 7.25 mM (CI: 3.11-16.90 mM), respectively ( Figure 4c). The B max of the high and low affinity binding sites were 21.3 (CI: 16.6-25.9) pmol/mg wet weight (426 pmol/mg dry weight), and 7.80 (CI: 6.43-9.17) nmol/mg wet weight (156 nmol/mg dry weight), respectively. We also examined the binding affinity of sodium ions by competition analysis, as sodium has similar characteristics to Cs ions and contamination of radioactive sodium chloride was reported in wastewater at the Fukushima site [1]. Sodium also bound to the two binding sites, and Ki values of 0.585 mM (CI: 0.052-6.64) and 92.1 mM (CI: 36.9-230.4) were obtained ( Figure 4c). Compared to Cs, the lower binding affinity of sodium ions could potentially be applied to the decontamination of 137 Cs from sodium chloride-contaminated water.
We collected soil and water samples that had been contaminated by radioactive fallout from Namie-machi, Fukushima (37u32930.850N, 140u51940.750E), a hot spot located 20 km from the nuclear plant. Average ground radioactivity measurements in the area were approximately 60 kcpm (Figure 5a). Recent reports have shown that soil deposition of 134 Cs, 137 Cs, 89 Sr, and 90 Sr in this area was 1.94610 3 , 2.21610 3 , 1.60610 4 , and 3.7610 3 kBq/ m 2 , respectively [15,16]_ENREF_14. 137 Cs detected in water samples and extracts from soil samples were approximately 85 and 79 Bq/ml, respectively, which is compatible with the reported 137 Cs radioactivity in this area, 2.21610 3 kBq/m 2 ( = 221 Bq/ cm 2 ). After incubation of algae with water samples or extracts from soil samples for 8 h, radioactivity from 137 Cs or gross b radioactivity in the pellet constituted by algae was quantified. 100 mg/ml of algal cells accumulated 63.0% of gross b radioactivity by incubation in water samples. Up to 47.9% of 137 Cs (c-ray) in water samples was also accumulated by 100 mg/ ml of Binos (Figure 5b). Similarly, in soil-water preparations, the alga accumulated both b-ray emitting radionuclides and 137 Cs by as much as 62.2% and 74.0%, respectively (Figure 5c).

Discussion
Here we report the characteristics of newly discovered green microalga, Parachlorella sp. binos (Binos), which has thick alginatecontaining extracellular matrix and abundant chloroplasts. Binos exhibited light-dependent uptake of radioiodine and highly efficient incorporation of the radioactive isotopes strontium and cesium in a light-independent manner. In addition, we show accumulating radioactive nuclides by Binos from water and soil samples collected from a heavily contaminated area in Fukushima.
Brown algal species, such as the kelp, Laminaria digitata, have widely been considered to be the most effective living accumulators of iodine, and tissue concentrations exceeding 50 mM have been reported [14]. In addition, marine brown algae have also been reported to absorb divalent or trivalent heavy metal ions, and B max values of approximately 120-2030 mM have been obtained [8]. Our findings show that Binos was capable of accumulating 50.4, 310, and 156 mM iodine, Sr, and Cs, respectively. Indeed, dried preparations of this algal strain exhibited absorption efficiencies that were comparable with those of brown algae. It was recently reported that Closterium moniliferum, a desmid green alga, accumulated Sr [17]. In this study, we report that this novel green algal strain, Binos, was capable of simultaneously adsorbing the most abundant radioisotopes released by the reactors at the Fukushima Daiichi Nuclear Power Plant. Development of decontamination systems that are both efficient and economical are urgently required in order to remediate areas affected by this accident and to reduce health risks resulting from radiation exposure.
Transmission electron microscopy of Binos revealed its unique characteristics, a thick alginate-containing extracellular matrix and abundant chloroplasts, both of which were not observed in Parachlorella kessleri [11]. Our study showed that radioiodine was accumulated into the cytosol under light exposure or in the presence of hydrogen peroxide. These findings suggest that iodine uptake by Binos was, at least in part, mediated by the increase in the oxidative power associated with photosynthesis. It is therefore possible that the abundance of chloroplasts in this algal strain can account for the efficient accumulation of radioiodine. In contrast to iodine, SIMS mapping of strontium clearly showed localized distribution of Sr at the outer surface of extracellular matrix. It may thus be possible to exploit the thick extracellular matrix to accumulate radioactive cations.
In the radionuclide uptake assay, we calculated the bound/free ratio that is important for estimation of decontamination efficiency. As shown in Figure 4b, the bound/free ratio of 137 Cs did not differ between the three groups with different amount of alga. This result suggests that the amount of cesium accumulated in algae is dependent on the constant bound/free ratio, and that the binding reaction with cesium might be reversible reaction. In contrast, the bound/free ratio of 85 Sr was increased paralleled with the amount of alga (Figure 3b). This result might be reflected by the higher affinity in strontium binding in comparison with that in cesium binding. Although the Bmax in cesium binding to low affinity binding site, 7.8 nmol/mg wet Binos (Figure 4c), was comparable with that in strontium binding, 15.5 nmol/mg wet Binos (Figure 3c). Affinity of strontium binding, Kd = 387 mM, was significantly higher in comparison with that of cesium binding to lower affinity binding site, Kd = 7.25 mM. In addition, the fact that a fraction of strontium was identified in the algal cytosol (Figure 3d-f) suggests that the binding reaction with strontium . Radioactivity accumulated in algal cells were measured, and data are shown as maximal uptake. b, Algal cells incubated with Na 125 I at room temperature under illumination for 24 h. Radioactivity accumulated in algal cells were measured, and data are shown as % of total radioactivity. *, P,0.05, **, P,0.01 (n = 4). c, After 24 h incubation, pellet and supernatant radioactivities were measured and the bound/free ratios of 125 I were calculated (n = 4). d, Ten mg/ml of wet algal cells were incubated with nonradioactive NaI and 1.48 kBq of Na 125 I (n = 4). Radioactivity accumulated in algal cells were measured, and data are shown as % of control uptake without nonradioactive NaI e, Algal cells were incubated with or without 10 mM of hydrogen peroxide (H 2 O 2 ) for 24 h (n = 3). f, g, After incubation of algal cells with 1 mM of sodium iodide for 24 h, elemental distribution of 12   . Binding of radioisotopes of strontium with P. sp binos. a, 10, 30, and 100 mg/ml of wet algal cells were incubated with 2.17 kBq of 85 SrCl 2 at room temperature for 10 min. Radioactivity accumulated in algal cells were measured, and data are shown as % of total radioactivity. *, P,0.0001 (n = 6). b, After 10 min incubation, pellet and supernatant radioactivities were measured and the bound/free ratios were calculated. *, P,0.0001 (n = 6). c, Ten mg/ml of wet algal cells were incubated with nonradioactive SrCl 2 (solid circles) or CaCl 2 (open circles) and 2.17 kBq of 85 SrCl 2 at room temperature for 10 min (n = 4). Radioactivity accumulated in algal cells were measured, and data are shown as % of control uptake without nonradioactive SrCl 2 . d, e, After incubation of algal cells with 1 mM of strontium chloride, elemental distribution of 12  might include somewhat of irreversible incorporation into the cell. In the case of 125 I, the bound/free ratio was in inverse proportion to the amount of algae (Figure 2c). When 10 mg of algae were incubated in the culture wells, light was supposed to expose to all algae. However, in such condition as incubation with much amount (100 mg) of algae, which were overlaid with each other in the incubation wells, mutual inhibition of light exposure to algae might decline the efficiency of uptake with decreased bound/free ratio. Therefore, we suggest that the true uptake efficiency might be shown in the results using 10 mg/ml of algae.
We collected soil and water samples in Namie-machi, Fukushima, where one of hottest spots located in the northwest of Fukushima Daiichi Nuclear Power Plant. We show that Binos accumulated 137 Cs and b-ray emitting radionuclides in the water and soil samples. In comparison with the water samples shown in Figure 5b, Binos accumulated 137 Cs and b-ray emitting radionu-clides more effectively in extracts from soil samples (Figure 5c). Soil and visible macro-debris were completely removed from extracts by three-times centrifugation. Therefore, it is unlikely that some soil components unbound to Binos settled down into pellets after incubation with Binos. We speculated that positively charged unvisible micro-debris containing radionuclides derived from soil might bind to negatively charged alginate in the extracellular matrix of Binos.
Zeolite is an aluminosilicate that also efficiently absorbs radioactine iodine, cesium, and strontium [18], and has been utilized in the water decontaminating system in the Fukushima Daiichi Nuclear Power Plant. However, the volume of zeolite is not able to be reduced furthermore. In this report, we demonstrate that this novel alga strain can simultaneously decontaminate all predominant radioisotopes released from Fukushima Daiichi nuclear power reactors, and that this strain can reduce the volume Figure 4. Binding of radioisotopes of cesium with P. sp binos. a, 10, 30, and 100 mg/ml of wet algal cells were incubated with 1.92 kBq of 137 CsCl at room temperature for 10 min. Radioactivity accumulated in algal cells were measured, and data are shown as % of total radioactivity (mean 6 SD). *, P,0.05, **, P,0.01 (n = 4). b, After 10 min incubation, the radioactivities of pellets and supernatant were measured and the bound/free ratios were calculated (n = 4). c, Ten mg/ml of wet algal cells were incubated with nonradioactive CsCl (solid circles) or NaCl (open circles) and 1.92 kBq of 137 CsCl at room temperature for 10 min. Radioactivity accumulated in algal cells were measured, and data are shown as % of control uptake without nonradioactive CsCl. Non-linear curve fitting to the model of two binding sites with high and low affinities was performed using the CsCl and NaCl competition data (n = 4). doi:10.1371/journal.pone.0044200.g004 Absorption of Radionuclides by a Novel Algae PLOS ONE | www.plosone.org of radioactive wastes at the ratio of 1:160-638 with the drying step. In addition, advantage to use this strain rather than any strains of natural seaweeds is that this strain can rapidly proliferate in industrial plants [9] and can be applied to various styles of decontamination systems because of its microscopic size. Since contaminated water in Fukushima Daiichi Nuclear Power Plant is expected to be in various conditions such as high concentration of salts, high temperature, low, or high pH, the characteristics of this strain that is resistant to hot, high salt, acidic, or alkaline condition must be another advantage. Development of decontamination system, which is expected to be efficient and economical, is urgent assignment to settle the accident and reduce risk of health hazard.

Sampling and Cultures of Algal Strains
We isolated a strain of green microalgae from activated sludge at a wastewater treatment plant in Kitaibaraki, Ibaraki, Japan, approximately 78 km from the Fukushima Daiichi Nuclear Power Plant. No specific permits were required for this studies and obtaining algal species. No specific permissions were required for the sampling location. We confirm that the location is not privately-owned or protected in any way, and that the field studies did not involve endangered or protected species. Algae were isolated from activated sludge by treatment with 0.1% povidone iodine. We isolated a strain of green microalgae that was viable at high temperatures up to 60uC and was resistant to acidic and alkaline conditions at a pH range of 3-11. The alga was cultured in inorganic growing medium containing 111 mM glucose, 2.5 mM KNO 3

Amplification of 18S rRNA Gene Sequences
Total DNA was extracted using an ISOPLANT kit (Nippon Gene, Tokyo, Japan) according to the manufacturer's instructions. 0.1 mg of purified DNA was used as a template for PCR, which was performed using the forward primer 59-GTAGTCA-TATGCTTGTCTC-39 and the reverse primer 59-GGCTGCTGGCACCAGACTTGC-39, with 30 cycles of 1 min at 94uC, 1 min at 55uC and 1 min for 72uC. Amplified PCR products were then ligated into p-GemT vectors (Promega, Madison, WI) and sequenced using an ABI3700 automated DNA sequencer (Life Technologies, Carlsbad, CA).

Amplification of Actin cDNA
Total RNA was extracted by phenol/chloroform precipitation after treatment of the algal cells with Proteinase K and DNase. mRNA was then reverse transcribed using a PrimeScript kit/ PrimeScript reverse transcriptase (Takara Bio, Otsu, Shiga, Japan) and the PCR was performed using cDNA as a template with the forward primer 59-ATGACSCAGATCATGTTYGAGAC -39 and the reverse primer 59-CCACATYTGCTGGAAGGTGG-39 for 30 cycles of 1 min at 94uC, 1 min at 50uC and 1 min at 72uC. Actin cDNA sequences were determined as described above.

Phylogenetic Analysis
The 18S rRNA and actin cDNA were amplified by reverse transcription-PCR as described above. rRNA nucleotide and actin amino acid sequences for the phylum Chlorophyta were obtained from the GeneBank database and were aligned using the MEGA5 software package [19].

Fourier Transform Infrared Spectroscopy
The extracellular matrix of the algal cells was precipitated by treating the supernatant of the incubation medium with ethanol Then, either the precipitated sample or purified sodium alginate (Nacalai Tesque, Kyoto, Japan) was analyzed using the attenuated-total-reflection technique with an IRPrestige-21 Fourier Transform Infrared Spectroscopy (Shimadzu Corporation, Kyoto, Japan).  ) in 24 well plates. After incubation with radioisotopes, the algae were centrifuged at 30006g for 10 min at 4uC and the weight and radioactivity of the pellet was determined after removal of the supernatant. Unless stated otherwise, data were presented as means 6 SD.

Radionuclide Uptake Assay
The bound/free ratio was calculated with the following formula.

Bound=free ration~(b=p)=(s=v)
where b is radioactivity accumulated by Binos (cpm), p is weight of the pellet (g), s is radioactivity of the supernatant (cpm), and v is volume of the supernatant (ml). For the competition assay, the algal strain was simultaneously incubated with radioactive isotopes and nonradioactive NaI, SrCl2 or CsCl in 1 ml of H 2 O. Non-linear curve fitting and calculation of B max , K d , IC 50 , and Ki was then performed using GraphPad Prism software (version 5.0d for Macintosh, GraphPad Software, San Diego, CA), and its statistical significance was analyzed with a t-test.

Secondary Ion Mass Spectroscopy (SIMS) Imaging
SIMS examinations were carried out on a NanoSIMS-50 Ion Microprobe (CAMECA, Courbevoie, France) [20]. To analyze the intracellular distribution of iodine, algal cells (10 mg/ml) were incubated with 1 mM of sodium iodide for 24 h. For imaging of strontium, algal cells were incubated with 1 mM of strontium chloride for 1 h. After centrifugation, the samples have been deposited on papers foils. The papers foils have been projected on copper block at N 2 temperature (2180uC). After that, the samples have been dehydrated by sublimation of the ice before being plunged in cooled (230uC) Unicryl resin. Resin infiltration has been carried out 24 h at -30uC. Afterwards UV polymerization was achieved 24 h at -30uC and 48 h by rising temperature to room temperature. The obtained resin blocs were sectioned by ultra microtome (400 nm) and deposited on NanoSIMS50 silicon wafer. SIMS observations were carried out on a NanoSIMS 50 micro beam analyzer using a Cs+ primary source. Distribution of negative ions: 12 C 14 N 2 , 88 Sr 35 Cl 2 , and 127 I 2 were recorded as a matrix of 256 6 256 image points.

Contaminated Water and Soil Sampling and Binding Assay
Water and soil samples were collected on 15 June 2011 in Namie-machi, an area that had been contaminated by radiation fallout from the Fukushima Daiichi Nuclear Power Plant 20 km away. Water samples were corrected from a small pool, in which water did not flow into or out. Soil samples were corrected at the shoulder of the road. At the time of collection, the radiation dose at this location was reported to be $20 mSV/h [21]. Radioactive nuclides from soil samples were extracted by incubating the samples at 80uC with equal quantities of H 2 O overnight [22]. Centrifugation of the water samples and soil-water preparations were performed three times at 3000 6g for 10 min to remove any soil or debris. The supernatant was then incubated with the Binos alga for 8 h, after which the samples were centrifuged at 30006g for 10 min at 4uC. Radioactivity from 137 Cs in the pellet constituted by Binos was quantified by measurement of c-ray in the energy region between 547 keV and 770 keV. Gross b radioactivity in the energy region between 500 keV and 2000 keV where b-ray from 137 Cs, 89 Sr, and 90 Sr were included were measured by a liquid scintillation counter.