Bacillus subtilis SepF Binds to the C-Terminus of FtsZ

Bacterial cell division is mediated by a multi-protein machine known as the “divisome”, which assembles at the site of cell division. Formation of the divisome starts with the polymerization of the tubulin-like protein FtsZ into a ring, the Z-ring. Z-ring formation is under tight control to ensure bacteria divide at the right time and place. Several proteins bind to the Z-ring to mediate its membrane association and persistence throughout the division process. A conserved stretch of amino acids at the C-terminus of FtsZ appears to be involved in many interactions with other proteins. Here, we describe a novel pull-down assay to look for binding partners of the FtsZ C-terminus, using a HaloTag affinity tag fused to the C-terminal 69 amino acids of B. subtilis FtsZ. Using lysates of Escherichia coli overexpressing several B. subtilis cell division proteins as prey we show that the FtsZ C-terminus specifically pulls down SepF, but not EzrA or MinC, and that the interaction depends on a conserved 16 amino acid stretch at the extreme C-terminus. In a reverse pull-down SepF binds to full-length FtsZ but not to a FtsZΔC16 truncate or FtsZ with a mutation of a conserved proline in the C-terminus. We show that the FtsZ C-terminus is required for the formation of tubules from FtsZ polymers by SepF rings. An alanine-scan of the conserved 16 amino acid stretch shows that many mutations affect SepF binding. Combined with the observation that SepF also interacts with the C-terminus of E. coli FtsZ, which is not an in vivo binding partner, we propose that the secondary and tertiary structure of the FtsZ C-terminus, rather than specific amino acids, are recognized by SepF.


Introduction
Bacterial cell division is mediated by a multi-protein machine known as the ''divisome'' that assembles into a ring at the site of cell division [1]. Formation of the divisome starts with the polymerization of the tubulin-like protein FtsZ into a ring, the Zring, just below the membrane at the cell division site. Because of its critical role in division, Z-ring formation is under tight control to ensure bacteria divide at the right time and place. Once formed, the Z-ring functions as a scaffold to which all other division proteins are recruited, and several proteins bind to the Z-ring to mediate its membrane association and persistence throughout the division process [2].
Placement of the Z-ring is mediated by two systems, that prevent cells from dividing at the poles or immediately after division is completed (Min), or from dividing through nucleoids (nucleoid occlusion). The MinC component of Min directly acts on FtsZ [3,4], whereas nucleoid occlusion is mediated by the FtsZbinding protein SlmA in E. coli [5,6] and the non-homologous protein Noc in B. subtilis, which does not seem to act directly on FtsZ [7]. In B. subtilis, Z-ring formation is also controlled by the nutritional status of the cell, through the nutritional sensor UgtP that acts directly on FtsZ [8]. Another level of control is provided by ClpX. In B. subtilis ClpX destabilizes, but not degrades FtsZ polymers, whereas in E. coli ClpX degrades (preferentially polymeric) FtsZ and thus controls the amount of FtsZ available for Z-ring formation [9,10].
The Z-ring is tethered to the membrane by FtsA. In E. coli, ZipA forms an additional membrane tethering factor [11]. A protein with a similar topology to ZipA, EzrA, acts as a destabilizer of Zrings in B. subtilis [12]. Z-rings are further stabilized by ZapA [13], a protein that strengthens lateral associations between FtsZ filaments, and in a small subset of bacteria including E. coli, ZapC fulfills a similar function [14,15]. Other proteins that interact directly with FtsZ have a specific function in division. FtsE functions as part of the FtsEX complex that controls hydrolysis of the cell wall in dividing E. coli [16,17]. In B. subtilis, SepF is a ringforming protein that binds FtsZ and plays a role in correct assembly of the cross-wall septum, presumably by bundling FtsZ polymers into ,50 nm wide tubes that can be observed in vitro [18]. During sporulation, SpoIIE interacts with FtsZ to ensure proper formation of the asymmetric septum [19] and MciZ, a 40 amino acid protein, prevents aberrant FtsZ ring formation in the mother cell compartment [20].
Even though crystal structures of FtsZ and various interacting proteins exist, relatively little is known about the precise interactions between FtsZ and these proteins. However, it is increasingly evident that a conserved stretch of amino acids at the C-terminus of FtsZ is involved in many of these interactions. The high degree of conservation of this C-terminal peptide was identified by Ma and Margolin [21]. The C-terminal peptide consists of a highly conserved core sequence followed by a variable number of poorly conserved residues at the extreme C-terminus. This region is not resolved in the available FtsZ structures, as it is preceded by a non-conserved, flexible linker sequence of variable length. The FtsZ-binding domain of ZipA has been co-crystallized together with a peptide matching the FtsZ C-terminus, which showed an extended b-strand followed by an a-helix [22]. In addition to ZipA, evidence has been reported for the interaction of the conserved FtsZ C-terminus with MinC [23], FtsA [24], EzrA [25], ClpX [9], SepF [26] and FtsZ itself [27]. Evidence for the interaction of FtsA with the Cterminus is based on yeast two-hybrid data [24]. For MinC, mutations in the FtsZ C-terminus conserved core have been described that abolish the interaction with MinC both in vivo and in vitro [23]. Using a C-terminal truncation of FtsZ (FtsZ Ec D18) that is still capable of polymerization, it was shown that ClpX is far less active in degradation of FtsZ Ec D18 than of wt FtsZ [9]. All these studies were done in E. coli. In Mycobacterium tuberculosis, the FtsZ C-terminus mediates the interaction between FtsZ and FtsW [28]. EzrA and SepF are Gram-positive division proteins that modulate FtsZ. The effects of EzrA or SepF on FtsZ polymerization can be relieved by adding competing amounts of a synthetic peptide encoding the FtsZ C-terminus, and EzrA and SepF have no effects on a C-terminal truncation mutant of FtsZ that still polymerizes (FtsZ Bs D16) [25,26]. The SepF study showed that SepF bundles FtsZ filaments and co-sediments with FtsZ in polymerization experiments. The FtsZ Bs D16 truncate still polymerized but no longer bundled in the presence of SepF, yet SepF still co-sedimented with FtsZ Bs D16, leading the authors to suggest that SepF also binds to a secondary site on FtsZ next to the C-terminus [26]. Recently the poorly conserved residues at the extreme C-terminus have been implicated in lateral interactions between FtsZ polymers that are potentially mediated by electrostatic forces [27].
The crystal structure of the FtsZ-binding domain of ZipA bound to a peptide matching the FtsZ C-terminus [22], and the recent crystal and NMR structures on the interaction of Thermotoga maritima FtsA (FtsA Tm ) and a FtsZ Tm C-terminal peptide [29] provide more insight in how the C-terminus is bound by proteins. Bound to ZipA, the C-terminal peptide assumes the confirmation of an extended b-strand followed by an a-helix, whereas bound to FtsA the peptide is predominantly helical, but not as a single helix. Six residues in the peptide interact with ZipA, mostly through hydrophobic contacts but also through two hydrogen bonds between the backbones of ZipA and the FtsZ-peptide, formed by a conserved Asp and Leu residues (D367 and L369 in B. subtilis FtsZ) [22]. The interactions with FtsA are quite different, with three salt bridges connecting the peptide to FtsA. The salt bridges are formed by a conserved Asp and Arg residue (D370 and L376 in B. subtilis FtsZ) and the carboxyl group of the C-terminal amino acid residue [29]. Based on a comparison of both structures it has been suggested that the FtsZ C-terminus can adopt different conformations to fit different binding pockets [29].
In this study, we describe a pull-down strategy to identify interaction partners of the B. subtilis FtsZ C-terminus. Using this strategy we identify SepF as a binding partner of the C-terminus, confirming an earlier observation of Singh et al. [26]. We extend the earlier results by showing that SepF only binds to the Cterminus and by identifying residues critical for binding of SepF by an alanine-scan of the FtsZ C-terminus.

SepF binds to the FtsZ C-terminus
To screen for B. subtilis proteins that interact with the FtsZ Cterminus we fused the last 69 amino-acid residues of B. subtilis FtsZ to a HaloTag (Halo-ZC, Fig. 1A). The HaloTag consists of a modified dehalogenase that can covalently bind to a chloroalkane ligand coupled to sepharose beads (HaloLink resin) [30]. As a control we fused only the flexible, non-conserved linker sequence of FtsZ without the final 16 amino acid residues to a HaloTag (Halo-ZDC16). The fusion proteins were expressed in E. coli and bound to the Halolink resin in binding buffer. Aliquots of the resin containing the covalently attached bait protein were subsequently used for pull-down experiments. To validate the pull-down approach we analyzed the binding of three candidate interacting proteins after overexpression in E. coli. EzrA and SepF have been reported to interact with the FtsZ Cterminus in B. subtilis, and MinC interacts with the FtsZ Cterminus in E. coli [23,25,26]. We expressed B. subtilis MinC fused to a strep-tag (MinC-strep), the cytosolic domain of EzrA fused to a His-tag (His-EzrA cyt ), and SepF fused to Maltose-Binding protein (MBP-SepF) in E. coli cells, prepared cell lysates and incubated the lysates with aliquots of resin with attached bait protein. After extensive washing, the bait protein and any interacting proteins bound to it were eluted from the resin by TEV protease cleavage. We found that MBP-SepF was retained by Halo-ZC bound to resin, and very weakly by Halo-ZDC16 bound to resin (Fig. 1B). This confirms an earlier report that suggested that SepF binds to the FtsZ C-terminus [26]. His-EzrA cyt and MinC-strep were not retained by either construct, also not when we tested for the presence of prey protein using specific antibodies against the His-or strep-tag (not shown). Previously, we had not been able to establish a direct interaction between B. subtilis MinC and FtsZ [31].

The interaction between the FtsZ C-terminus and SepF is specific
To study the interaction between SepF and the FtsZ C-terminus in more detail, and to exclude that the interaction is mediated by potential 'bridging' proteins present in the E. coli lysate, we repeated the pull-down experiments with purified MBP-SepF and SepF. As an extra control, we used a bait construct in which a conserved proline residue in the C-terminus was mutated into alanine, Halo-ZC P372A . Pro372 (notation for full length FtsZ) is fully conserved in the FtsZ C-terminus across species and we reasoned that a mutation of this residue would abolish the capability of the C-terminus to interact with SepF. Both pure MBP-SepF and SepF were retained when Halo-ZC was used as bait protein. When Halo-ZDC16 or Halo-ZC P372A were used as bait a very faint band of MBP-SepF was visible in the eluate, but untagged SepF was not retained ( Fig. 2A). This confirms that the interaction between SepF and the FtsZ C-terminus is specific, and that the pull-down is not caused by aspecific binding of the MBPmoiety to the C-terminus. The result also shows that the conserved proline residue indeed is critical for the function of the FtsZ Cterminus. We noticed that the ZC P372A bait protein is visible in the eluate as two bands on gel. The protein is initially expressed as fulllength protein, during elution the ZC P372A bait is released from the Halo-tag by TEV cleavage. The double band pattern can be attributed to a change in the electrophoretic pattern of the mutated bait protein which is not completely resolved by the sample buffer resulting in two bands. Another, less likely explanation is that the alanine substitution gives rise to an extra TEV cleavage site. Other alanine substitutions in the C-terminus also give rise to double bands for the bait protein, and in several cases this change did not affect SepF binding (see below).
MBP-SepF bound to amylose resin can be used to pull down FtsZ from E. coli lysates expressing B. subtilis FtsZ [18]. This allowed us to perform the reverse pull-down experiment, and we incubated MBP-SepF bound to resin with purified FtsZ, FtsZDC16 and FtsZ P372A . Importantly, in this experiment fulllength FtsZ was used, so if there is a secondary binding site for SepF on FtsZ, one could expect that all three prey proteins are pulled down by MBP-SepF. We found that wild type FtsZ was bound by MBP-SepF and could be detected in the eluted fraction both by Coomassie staining and by western blotting (Fig. 2B). FtsZDC16 and FtsZ P372A were not visibly co-eluted with MBP-SepF with Coomassie, and only a very faint band was detected for co-eluted FtsZ P372A . This result suggests that the FtsZ C-terminus is either the only site of interaction with SepF, or that the binding of SepF to the secondary binding site is weak.
Two mutations in SepF, SepF A98V and SepF F124S have been described that abolish SepF-FtsZ interaction [18]. We purified MBP-SepF A98V and MBP-SepF F124S and used these proteins as prey in a Halo-ZC pull-down. Neither SepF mutant was found to bind to the FtsZ C-terminus (Fig. 2C). This suggests that the mutations in SepF A98V and SepF F124S abolish binding of SepF to the FtsZ C-terminus rather than to a secondary binding site on FtsZ. It is also of note that whereas MBP-SepF runs as monomers and dimers on SDS-PAGE, the two mutants do not, confirming the observation that both mutants, most notably SepF A98V , are less prone to form large oligomeric structures [18].
Taken together, these results show that SepF interacts with the FtsZ C-terminus, that this interaction is specific, and that we cannot find evidence for a secondary interaction site on FtsZ using pull-down assays.

SepF cannot tubulate FtsZDC16 and FtsZ P372A polymers
In vitro, SepF rings and FtsZ polymers combine to form large tubular structures [18]. Our pull-down experiments show that FtsZDC16 and FtsZ P372A have lost the ability to bind to SepF, but we cannot exclude that a weak residual interaction between the proteins (e.g. through a secondary interaction site on FtsZ) still allows the formation of tubules. Therefore, we mixed FtsZ, FtsZ P372A and FtsZDC16 with SepF and GTP, to induce FtsZ polymerization and possible tubule formation, and examined the samples using electron microscopy. FtsZ formed tubules with SepF (Fig. 3D), but in the case of FtsZ P372A and FtsZDC16 we could detect both FtsZ polymers and SepF rings (Fig. 3E, F), but not tubules, despite careful examination of the sample grids. We noticed that under our experimental conditions, FtsZ P372A readily polymerized, but that FtsZDC16 only formed very short polymers and aggregates (Fig. 3A-C), unlike the single stranded filaments that were reported for a FtsZDC17 mutant [27]. We attribute this difference to the buffer system used in our study, with an increased pH and salt concentration that has previously been reported to decrease the amount of FtsZ polymerized in centrifugation and light scattering assays [18].
As a second assay for tubule formation we used sedimentation, at a velocity whereby SepF-FtsZ tubules but not FtsZ polymers are recovered in the pellet. FtsZ and FtsZ P372A were polymerized with GTP in the presence and absence of SepF. FtsZDC16 was not used in this assay because of its tendency to aggregate. FtsZ was recovered in the pellet above background levels only when SepF was present, whereas FtsZ P372A was not pelleted with SepF (Fig. 3G, H). SepF sedimentation is dependent on the presence of FtsZ, and does not occur with FtsZ P372A , or when SepF is incubated alone (Fig. 3G). Combined with the pull-down experiments above this provides evidence that the FtsZ C-terminus is the only site of interaction with SepF.
The SepF binding site arises from the folding of the FtsZ C-terminus The Halo-ZC construct specifically pulls down SepF, and this interaction is lost upon deletion of the final 16 residues from the Cterminus or by mutating the conserved Pro372 to Ala. To learn which other residues in the conserved C-terminus are critical for SepF binding, we performed an alanine-scan of the C-terminus. Each of the last 16 residues of the C-terminus was mutated to Ala (Fig. 1A), resulting in a series of HaloZC Ala constructs which were used to pull down purified SepF (Fig. 4A). We determined the intensity of Coomassie-stained bands from the eluted C-terminus and the co-eluted SepF, and plotted the ratio of the densities as an indication for binding, using Halo-ZC and Halo-ZDC16 as references (Fig. 4B). The use of ratios of bound SepF to eluted bait protein means that we have corrected for possible fluctuations in the amount of the bait protein bound to and/or eluted from the beads. Changing the fully conserved Pro372 and Phe374 residues abolished SepF binding. Changing Leu369, Leu375, Arg376 and Asn377 reduced SepF binding to less than 25% of the binding observed with the wild type. Changing Asp370, Ile371, Arg378, Lys380 and Arg381 also notably affected SepF binding, whereas changing Asp367, Thr368, Thr373, Asn379 and Gly382 resulted in SepF recovery that was not noticeably different from wild type.
The fact that 11 out of 16 mutations inhibited SepF binding to various extent, suggests that the recognition of the FtsZ Cterminus by SepF depends more on the folding of the C-terminus than on specific interactions between amino acids in the form of salt bridges.
The E. coli FtsZ C-terminus has a conserved part that is very similar to that of B. subtilis FtsZ (Fig. 5A), yet as E. coli does not have SepF there is no need for FtsZ Ec to bind SepF. We reasoned that if the overall conformation of the C-terminus is recognized by SepF, then SepF might recognize the E. coli FtsZ C-terminus as well. We constructed Halo-ZC Ec , Halo-Z Ec DC14 and Halo-ZC EcP375A similar to our B. subtilis constructs and performed a pull-down with purified SepF (Fig. 5B). SepF was bound to the Halo-ZC Ec construct but not by Halo-Z Ec DC14 or Halo-ZC EcP375A . This result shows that SepF is capable of recognizing the C-terminus of a homologous FtsZ molecule, although this homologous FtsZ is not a binding partner for SepF in vivo.

Discussion
In this paper we describe a novel pull-down assay to detect protein-protein interactions of the FtsZ C-terminus. The assay is based on the HaloTag fusion technology in which the bait protein is covalently bound to a resin allowing stringent wash steps before applying a sample to the resin [30]. Our fusion constructs consist of a modified dehalogenase fused to a part of FtsZ containing both the long flexible, non-conserved linker sequence and the conserved FtsZ C-terminus. The presence of the linker sequence has a dual function, it provides flexibility and distances the C-terminus from the dehalogenase, and it provides a control for unspecific binding through the FtsZDC constructs. Using our assay we can specifically pull down FtsZ binding partners.
To validate our pull-down strategy we performed pull-downs with the C-terminus of B. subtilis FtsZ on lysates of E. coli cultures expressing three B. subtilis proteins that have previously been reported to bind to the FtsZ C-terminus, EzrA [25], SepF [26] and MinC. It has to be noted that evidence for MinC binding to the FtsZ C-terminus comes from the homologous E. coli proteins [23]. Although binding for each protein could be expected, we only managed to pull down SepF. Although genetic evidence suggested that MinC Bs needs MinD Bs to act on FtsZ Bs [32] our earlier work showed that MinC Bs alone destabilizes bundles of FtsZ Bs polymers and increases the light scattering signal of non-polymerized FtsZ Bs in solution, indicating that FtsZ Bs and MinC Bs interact without MinD Bs [4]. However, we had not been able to detect interactions between full length MinC Bs and FtsZ Bs through a cross-linking approach [31], whereas MinC Ec cosediments with FtsZ Ec polymers [3] and polymerized FtsZ Ec can be recruited to MinC Ec / MinD Ec coated vesicles in a FtsZ Ec -C-terminus dependent manner [23]. This suggests that the interaction between B. subtilis FtsZ and MinC is not as strong as between the E. coli proteins. We do not know why we have not been able to pull down EzrA, as previously a peptide corresponding to the C-terminal 17 amino acids of FtsZ was shown to competitively inhibit EzrA binding to FtsZ and bound to EzrA with a dissociation constant of 102 mM [25]. The interaction between FtsZ and EzrA is sensitive to increasing salt concentrations [25] but our pull-down assays were performed at 150 mM NaCl, a salt concentration at which at least 50% of binding should still occur. Possibly we need a higher expression of His-EzrA cyt or to perform the pull-down assay under cross-linking conditions, or both, to detect the interaction between EzrA and the FtsZ C-terminus.
We have shown that our pull-down assay is very specific. Only SepF was pulled down from an E. coli lysate and control experiments showed that this pull-down was dependent on the presence of the C-terminus and independent of the tag attached to SepF. Furthermore, we could not detect SepF mutants that have lost the capability to bind FtsZ and we could show that FtsZ recovery in the reverse pull-down experiment depended on the presence of the FtsZ C-terminus (Fig 2). Our results strongly suggest that the FtsZ C-terminus is the only binding site for SepF. In an earlier study SepF has been found to co-precipitate with FtsZDC16 polymers, which led the authors to suggest a potential secondary binding site for SepF on FtsZ [26]. We attribute the observed co-sedimentation of SepF with FtsZDC16 polymers [26] to the buffer conditions used by the authors -25 mM Pipes at pH 6.8. In an earlier report we have shown that SepF forms rings at pH 7.5 and high salt, but precipitates as aggregates at pH ,7 [18]. In vitro polymerization and tubulation experiments confirmed that the formation of FtsZ-SepF tubules depends on the presence of the FtsZ C-terminus (Fig 3). SepF sedimentation is dependent on the presence of FtsZ polymers, and does not occur when we use polymers of FtsZ P372A or SepF alone (Fig. 3G).
To identify residues in the FtsZ C-terminus involved in SepF binding we performed an alanine-scan of the C-terminus and performed pull-down assays. Mutation of the two residues that are totally conserved across species, Pro372 and Phe374, abolished the FtsZ-SepF interaction. In total, 11 out of the 16 mutations tested considerably affected SepF binding to the C-terminus. This suggests that the secondary and teriary structure of the FtsZ Cterminus is recognized by SepF, in a way that is more similar to how ZipA binds FtsZ, through hydrophobic contacts and hydrogen bonds to the peptide backbone, rather than to how FtsA binds FtsZ, through specific salt bridges [22,29]. We cannot exclude that our mutations alter the structure of the C-terminus in such a way that residues are no longer in the right conformation to form salt bridges -for that structural information is required. Recently, the FtsZ C-terminus was divided into a conserved Cterminal tail (CTT) that is followed by a highly variable -both in length and composition -set of residues, the C-terminal Variable region (CTV) [27]. The CTT is thought to constitute the 'landing pad' for FtsZ-binding proteins whereas the CTV plays a role in promoting lateral interactions between FtsZ polymers [27]. The B. subtilis FtsZ CTV comprises residues 377-382. We noted that several residues in the CTV are likely to play a role in SepF binding, as alanine mutations of residues 377, 378, 380 and 381 led to a considerable decrease in SepF binding. Also, the salt bridge between the C-terminal residue of T. maritima FtsZ, which has a 7 residue long CTV, and FtsA [29], suggests that the CTV is part of the 'landing pad' for interacting proteins, next to its role in promoting lateral interactions. However, the finding that the E. coli FtsZ C-terminus, with a different and noticeably shorter CTV, also binds SepF, supports the notion that the CTT is the main binding partner for FtsZ-binding proteins. More information on the interactions between the FtsZ C-terminus and several FtsZbinding proteins is required to elucidate this question.
In conclusion, we have developed a novel and highly specific assay to identify interaction partners of the C-terminus of FtsZ from B. subtilis and E. coli, and validated the assay with candidate binding proteins. Our results show that SepF specifically binds the C-terminus whereas the interactions of B. subtilis MinC and EzrA are too weak to be detected in our assay. We are currently using this assay to look for novel proteins interacting with the FtsZ Ctermini from lysates of B. subtilis and E. coli cultures.

Plasmid construction
All plasmids are listed in Table 1. The sequence coding for the C-terminal 67 amino acid residues (317-383) from E. coli ftsZ was amplified from E. coli K12 chromosomal DNA by PCR using primers djs414 and djs415 (primers listed in Table 2). The sequence coding for the C-terminal 69 amino acid residues (314-382) from B. subtilis ftsZ was amplified from B. subtilis 168 chromosomal DNA by PCR using primers djs416 and djs417. Both PCR products were digested with PmeI and SgfI and ligated into PmeI/SgfI digested pFN18A (Promega), resulting in plasmids pDJ64 (E. coli ftsZ) and pDJ65 (B. subtilis ftsZ). pDJ65 was used as a template for site directed mutagenesis according to the Quickchange protocol (Stratagene), to introduce a stop codon at  the position of D367, and to systematically change residues 367 to 382 to alanine, using primers djs426 to djs459. Similarly, pDJ64 was used as a template for site directed mutagenesis, to introduce a stop codon at the position of D370, and to change residues P375 to alanine, using primers djs461 to djs464. The sequence coding for the soluble part of B. subtilis EzrA (amino acid residues 27-562) was amplified from B. subtilis 168 chromosomal DNA by PCR using primers ek13 and ek14. The PCR product was digested with NcoI and XbaI and ligated into NcoI/XbaI digested pET302, resulting in plasmid pEK5. All constructs were verified by sequencing.

Protein purification
MBP-SepF and SepF were purified as described [18]. FtsZ was purified as described [4], FtsZDC16 and FtsZ P372A were purified using the same protocol.

Pull down experiments
Expression of HaloTagged constructs was done in E. coli BL21(DE3) carrying plasmid pBS58 which encodes an extra copy of ftsQAZ [33]. Freshly transformed cells were grown overnight at 37uC, diluted 1:100 and grown to an OD 600 ,0.7 and induced with 1 mM IPTG. After three hours of growth cells were harvested by centrifugation and washed with PBS. Cell pellets were flash frozen in liquid nitrogen and stored at 220uC. To prepare lysates, cells were taken up in 1/10 th of the original volume in buffer HPB (50 mM HEPES pH 7,5; 150 mM NaCl). Lysozyme (0,1 mg/ml) and DNAse (1 mg/ml) were added and the suspensions were incubated for 10 min on ice. Cells were disrupted using sonication (12 cycles of 5s with 5s cooling at 10 microns probe amplitude). Aliquots of 1ml were made and stored at 220uC. For a typical pull down experiment, for every construct tested a 1 ml aliquot was thawed on ice and subsequently centrifuged at 100006g for 30 min at 4uC. DTT (to 1 mM) and Nonidet P-40 (to 0,005% v/v) were added to the supernatant. The rest of the procedure was carried out in buffer HPB+ (50 mM HEPES pH 7,5; 150 mM NaCl; 1 mM DTT; 0,005% v/v nonidet P-40). To the supernatant, 200 ml (25%) of HaloLink resin (Promega) equilibrated with HPB+ was added. The samples were incubated for 1 h at RT with gentle mixing. Subsequently, the samples were centrifuged at 10006g for 5 min at RT. The supernatant was discarded and the resin was washed 3 times with HPB+. The resin with bound HaloTagged bait protein were subsequently incubated with 1 mL cell lysates (106 concentrated with respect to the original culture volume) of E. coli strains overexpressing potential binding partners. Alternatively, the resin with bound Halo-tagged bait protein was incubated with 1 mL of MBP-SepF, MBP-SepF A98V , MBP-SepF F124S or SepF at 15 mM diluted in HPB+. For the alanine-scanning experiments, 300 mL of 30 mM SepF was used. The samples were incubated for 1 h at RT with gentle mixing. Subsequently, the samples were centrifuged at 10006g for 5 min at RT, and the supernatant was kept as the flow-through fraction. The resin was washed 3 times with HPB+. The HaloTagged bait protein and associated proteins were released from the resin by TEV-cleavage using 50 mL of Cleavage solution (HPB+ containing TEV-protease, Promega) for 1 h at RT with gentle mixing. The eluted material was collected by centrifugation at 10006g for 5 min at RT and the resin was washed with an additional 50 mL of HPB+ which was collected by centrifugation and added to the eluted material. Samples were prepared for SDS-PAGE and run on 15% gels, which were stained with Coomassie Brilliant Blue.
For the FtsZ pull-downs with MBP-SepF, MBP-SepF bound to amylose-resin was prepared as described [18]. 250 mL aliquots of amylose resin with MBP-SepF were incubated with 300 mL of 30 mM FtsZ, FtsZDC16 and FtsZ P372A in buffer A (20 mM Tris-HCl pH 7.4, 200 mM KCl, 1 mM EDTA). The proteins were allowed to bind to the resin for 30 min at 4uC. Unbound protein and liquid was removed by mild centrifugation and collected as Flow-through. The resin was washed 6 times with buffer A and the last wash fraction was collected. MBP-SepF and bound FtsZ were eluted from the resin with 300 mL of 10 mM maltose in buffer A. Equal amounts of the input material, the flow-through, last wash and eluate were loaded on 12% SDS-PAGE gels, which were either stained with Coomassie Brilliant Blue, or subsequently transferred to PVDF using Western Blotting and developed using anti-FtsZ.
GTP was added to a final concentration of 2 mM to start FtsZ polymerization and the samples were incubated for 20 min. at 30uC. 2 ml of each sample was applied onto a glow-discharged 400 mesh carbon-coated copper grid, incubated for 30 s., blotted dry