Alteration of POLDIP3 Splicing Associated with Loss of Function of TDP-43 in Tissues Affected with ALS

Amyotrophic lateral sclerosis (ALS) is an adult-onset neurodegenerative disease caused by selective loss of motor neurons. In the ALS motor neurons, TAR DNA-binding protein of 43 kDa (TDP-43) is dislocated from the nucleus to cytoplasm and forms inclusions, suggesting that loss of a nuclear function of TDP-43 may underlie the pathogenesis of ALS. TDP-43 functions in RNA metabolism include regulation of transcription, mRNA stability, and alternative splicing of pre-mRNA. However, a function of TDP-43 in tissue affected with ALS has not been elucidated. We sought to identify the molecular indicators reflecting on a TDP-43 function. Using exon array analysis, we observed a remarkable alteration of splicing in the polymerase delta interacting protein 3 (POLDIP3) as a result of the depletion of TDP-43 expression in two types of cultured cells. In the cells treated with TDP-43 siRNA, wild-type POLDIP3 (variant-1) decreased and POLDIP3 lacking exon 3 (variant-2) increased. The RNA binding ability of TDP-43 was necessary for inclusion of POLDIP3 exon 3. Moreover, we found an increment of POLDIP3 variant-2 mRNA in motor cortex, spinal cord and spinal motor neurons collected by laser capture microdissection with ALS. Our results suggest a loss of TDP-43 function in tissues affected with ALS, supporting the hypothesis that a loss of function of TDP-43 underlies the pathogenesis of ALS.


Introduction
Amyotrophic lateral sclerosis (ALS) is an adult-onset neurodegenerative disease accompanied by loss of motor neurons. A pathological hallmark of ALS is cytoplasmic inclusions in neurons and glia consisting of TAR DNA-binding protein of 43 kDa (TDP-43) [1,2,3]. Although TDP-43-positive inclusions have been identified in several neurodegenerative disorders, the discovery of mutations in the TARDBP gene in patients with familial and sporadic ALS (OMIM 612069; ALS10) indicates that an alteration of TDP-43 causes selective motor neuron degeneration [4,5]. The similarity between the pathological findings in ALS10 and sporadic ALS indicate that the alteration of TDP-43 may play an important role in the pathogenesis of sporadic ALS [3,6]. However, the way in which the molecular mechanism of this alteration of TDP-43 causes ALS is still obscure.
TDP-43 is a ubiquitously expressed nuclear protein and the clearance of nuclear TDP-43 in the affected neurons and glia is another pathological hallmark of ALS, suggesting that a loss of function of TDP-43 may underlie the pathogenesis of the ALS [2,3,7]. TDP-43 has two RNA recognition motifs (RRM1 and RRM2) and has been speculated as a heterogeneous nuclear ribonucleoprotein (hnRNP) [8,9]. TDP-43 binds to hnRNPs and functions in RNA metabolism through regulation of transcription, mRNA stability, and alternative splicing of pre-mRNA [10,11]. TDP-43 takes an important role for cell proliferation, neurite outgrowth and neuronal cell viability [12,13]. In animal models, several lines of evidence have revealed that TDP-43 is essential for normal embryogenesis, ES survival and takes an important role for motor neuron function [14,15,16,17]. However, a function of TDP-43 in the human tissues has not been well evaluated.
Controversy centers on whether a loss of TDP-43 function or an adverse effect of inclusions of TDP-43 results in motor neuron death in ALS [7]. The disappearance of nuclear TDP-43 in the affected motor neurons with TDP-43 inclusions supports the hypothesis that a loss of TDP-43 function may result in the motor neuron death in ALS [1,2,18]. However, TDP-43 function in tissues affected with ALS has not been evaluated. To explore the possibility that a loss of TDP-43 function may result in the motor neuron death in ALS, a function of TDP-43 in tissues affected with ALS should be elucidated. In this report, we first attempted to identify the molecular indicators of a function of TDP-43 in human cells, with special attention to pre-mRNA splicing. Then we evaluated a function of TDP-43 in the affected tissues with ALS by using the identified molecular indicator, splicing variants of POLDIP3.

Results
Comprehensive screening of TDP-43 functions in pre-mRNA splicing For investigating the function of TDP-43 in several tissues, ideal molecular indicators are the genes that are ubiquitously expressed and influenced by the depletion of TDP-43. TDP-43 has been reported to bind the intronic or noncoding sequences, which are markedly different between species, and affect pre-mRNA splicing [19,20]. Therefore we attempted to identify the genes which are influenced by TDP-43 by comparing the results of two differential human cells, HeLa and SH-SY5Y. siRNA treatment completely suppressed the expression of TDP-43 in HeLa cells, in contrast to ,30% in SH-SY5Y cells compared to control siRNA (Fig. 1A). We obtained the cDNAs from mRNA of these cells and applied them to GeneChip Human Exon 1.0 ST Arrays, subsequently identifying genes that are significantly altered in their splicing and amount of expression. We found 892 genes in HeLa cells and 103 genes in SH-SY5Y cells that altered the pattern of splicing under the depletion of TDP-43 (Fig. 1B). In contrast, we found 123 genes, 98 genes upregulated and 25 genes down regulated, in HeLa cells and 10 genes, 3 genes upregulated and 7 genes downregulated, in SH-SY5Y cells in which the amounts of mRNA were significantly altered more than 2-fold by TDP-43 siRNA compared to control siRNA.

POLDIP3 exon 3 is excluded by depletion of TDP-43
Among the genes that altered their splicing, we found that only 15 genes overlapped between both cell lines ( Fig. 1B Table 1). Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) analysis using primers specific for splicing variants revealed that only the POLDIP3 and HP1BP3 genes showed altered splicing consistent with the results of an exon array in both HeLa cells and in SH-SY5Y cells ( Fig. 1C and D, Fig. S1A and B).
We were interested in POLDIP3 because POLDIP3 showed the highest fold change by qRT-PCR in both cell lines (Fig. 1E). The mean gene-normalized probe-set signals for POLDIP3 revealed the lack of exon 3 as a result of the depletion of TDP-43 ( Fig. 2A). RT-PCR analysis using primers spanning exon 3 revealed that the cells treated with TDP-43 siRNA showed only one product; subsequent sequence analysis showed that it was from the transcripts excluding POLDIP3 exon 3 ( Fig. 2B and C). We designated the POLDIP3 gene lacking exon 3 as POLDIP3 variant-2 and wild-type as variant-1.
We also quantified the amounts of POLDIP3 splicing variants mRNA in various human cultured cells, HeLa, SH-SY5Y, and U87-MG, by qRT-PCR using primers that specifically amplified each splice variant. Under treatment with TDP-43 siRNA, the total amount of POLDIP3 in these cells was not significantly altered, whereas variant-1 was significantly reduced to ,5% and variant-2 was increased to more than 4 to 6 times compared to the control ( Fig. 2D and E). Similar results were obtained using two other mixtures of TDP-43 siRNAs ( Fig. 2F and G).
RNA binding ability of TDP-43 is necessary for including POLDIP3 exon 3 To investigate whether TDP-43 directly regulates the inclusion of exon 3 of POLDIP3, we first examined whether TDP-43 binds to the POLDIP3 mRNA by RNA-immunoprecipitation (RIP) assay using TDP-43 antibody. POLDIP3 mRNA was amplified in the material immunoprecipitated with anti-TDP-43 antibody (Fig. 3A, lane 4, top panel).
Next, we applied a supplementation assay by introducing the exogenous mutant TDP-43 in the cell depleted of endogenous TDP-43 by using siRNA targeted to the 39-UTR of TDP-43. TDP-43 has two RNA recognition motifs (RRM1 and RRM2) and the substitutions of F147L and F149L in the RRM1 motif decreased the binding ability to RNA, but the substitutions of F194L, F229L, and F231L in the RRM2 motif did not (Fig. 3B) [8]. We treated the HeLa cells by siRNA targeted to the 39-UTR of TDP-43 then transfected the various TDP-43 cDNAs and quantified the amount of POLDIP3 variants by real-time qRT-PCR. Although exogenous expression of TDP-43 wild-type and each mutant did not affect the expression of POLDIP3 total and variant-2 mRNA ( Fig. 3E and F), wild-type TDP-43 restored the amount of POLDIP3 variant-1 ( Fig. 3C and D, lane 3). In contrast, TDP-43 RRM1 mutant, but not RRM2 mutant, failed to restore the amount of POLDIP3 variant-1 (Fig. 3D, lanes 4 and 5). These results suggest that TDP-43 includes exon 3 of POLDIP3 by binding to its pre-mRNA.

Alteration of POLDIP3 splicing in the CNS of patients with ALS
To investigate the function of TDP-43 in tissues affected with ALS, we analyzed the amount of each POLDIP3 splicing variant mRNA. We isolated total RNAs from the thalamus, motor cortex and spinal cord of patients with ALS and control individuals (detailed information about subjects is summarized in Table S1) and quantified the amounts of mRNA of POLDIP3 splicing variants using real-time qRT-PCR. The total amounts of POLDIP3 and POLDIP3 variant-1 mRNA were not significantly different between control and ALS tissues (Fig. 4A). In contrast, the amount of POLDIP3 variant-2 was significantly increased in ALS tissues compared with controls (Fig. 4A). An increase of POLDIP3 variant-2 mRNA was also observed in the thalamus, which has not been thought to be involved in ALS. However, consistent with the previous report, TDP-43 pathology was observed in four out of six ALS cases used in this experiment [22] (Fig. S2).
Next we investigated the amounts of POLDIP3 mRNA in the spinal motor neurons of patients with ALS. We isolated at least TDP-43 Related Splicing Alteration in ALS Tissues PLOS ONE | www.plosone.org 100 spinal motor neurons at L2 in each individual with ALS and controls by laser capture microdissection. The extracted RNA was retrotranscribed, pre-amplified, and subsequently analyzed by real-time qRT-PCR. To assess the purity of the motor neuronenriched material, we quantified the amount of choline acetyltransferase (ChAT) mRNA, one of the markers for motor neurons, and compared it to neighboring cells. Similar expression levels of ribosomal protein, large P1 (RPLP1), which was one of the internal controls used in this experiment, was detected between them. In contrast, the expression of ChAT mRNA was only detected in the motor neuron-enriched material (Fig. 4B). The amount of expression of POLDIP3 variant-1 in spinal motor neurons was similar between ALS and controls (Fig. 4C). However, the amounts of expression of POLDIP3 variant-2 in ALS tissues was statistically increased over 4 times compared to controls (Fig. 4C).

The function of POLDIP 3 variant-2
Finally, we were interested in the function of POLDIP3 variant-2. POLDIP3 has been reported to enhance mammalian target of rapamycin (mTOR)/S6 protein kinase 1 (S6K1)-mediated translation efficiency of mRNA [23]. mTOR/S6K1 signal functions in cell proliferation and growth [24]. Indeed, the depletion of POLDIP3 results in decreased cell size [25]. Therefore, to estimate the function of POLDIP3 variant-2, we investigated the cell size in a neuronal cell line, SH-SY5Y, with or without TDP-43 siRNA. In SH-SY5Y cells treated with TDP-43 siRNA, cell size was significantly decreased and was comparable to depletion of POLDIP3 ( Fig. 5A and B) [25]. In contrast, in HeLa and HEK293T cells treated with TDP-43 siRNA, cell size was significantly increased, which is consistent with a previous report ( Fig. S3A and B) [26]. In addition, although there was no statistical significance, cell size in U87-MG cells treated with TDP-43 siRNA showed a tendency to be larger (Fig. S3C).
To further elucidate the functional difference between POL-DIP3 variant-1 and POLDIP3 variant-2, we investigated whether these POLDIP3 variants can restore the size of SH-SY5Y cells treated with TDP-43 siRNA. We transfected SH-SY5Y cells, which stably expressed the GFP-tagged POLDIP3 variant-1 or variant-2, with control or TDP-43 siRNA. Then we calculated the cell size recovery rate by comparing the cell size between the cells with or without the expression of GFP-tagged POLDIP3 variants ( Fig. 5C; Fig. S4). TDP-43 siRNA treatment suppressed the expression of TDP-43 to ,20% in both POLDIP3 stable cell lines compared to control siRNA (Fig. 5D). Although both POLDIP3 variants improved the cell size reduction caused by depletion of TDP-43, the recovery rate of cell size was higher in variant-1expressing cells than in variant-2-expressing cells and reached statistical significance, indicating that the POLDIP3 variant-2 is less effective at maintaining the size of SH-SY5Y cells than variant-1 (Fig. 5E).

Discussion
Here, we show the increasing the amounts of the POLDIP3 variant-2 mRNA in the affected tissues with ALS, including spinal motor neuron. The result suggests that a function of TDP-43 decreases in the affected tissues with ALS. The finding that POLDIP3 variant-2 increased in the cultured human cells depleted of TDP-43 is consistent with the previous reports [19,20]. In addition, we and Fiesel et al. found that RNA binding ability of TDP-43 is necessary for inclusion of exon 3 of POLDIP3, indicating that TDP-43 directly regulates splicing of POLDIP3 [26]. Therefore we conclude that the alteration of splicing of POLDIP3 represents the dysfunction of TDP-43. The finding that the TDP-43 function decreases in the affected tissue with ALS supports the hypothesis that a loss of function of TDP-43 underlies the pathogenesis of ALS.
We were not able to show the decreasing the amounts of POLDIP3 variant-1 in the affected tissues with ALS. There is a possibility that the increasing the amount of POLDIP3 variant-2 might be resulted from the mechanism which is not associated with the TDP-43 function. However, the reason why the decreasing the amounts of POLDIP3 variant-1 was not observed in affected tissues with ALS is related to the number of the neurons and glial cells in which POLDIP3 splicing altered. The alteration of POLDIP3 splicing may occur only in the cells with disappearance of nuclear TDP-43. The number of these cells is relatively small even though in the spinal motor neurons in ALS [27]. In addition, the amount of POLDIP3 variant-1 is abundant in normal cells, thus it would be difficult to detect the decreasing the amounts of POLDIP3 variant-1. In contrast, the POLDIP3 variant-2 is nearly absent in normal cells (Fig. 2B), thus increasing the amounts of POLDIP3 variant-2 is detected sensitively. The immunohistochemical analysis to compare the amount of variant-1 between the cells with or without nuclear TDP-43 should clarify this issue; however, the POLDIP3 antibodies evaluated in this study were not suitable for immunohistochemical analysis in human autopsied tissues (data not shown). Further study by using the specific antibodies for POLDIP3 variant-1 is necessary to elucidate this issue.  TDP-43 functions in exon inclusion as well as exon exclusion [19,20]; however, the precise mechanism for regulation of splicing by TDP-43 is unclear. TDP-43 binds to the intronic or noncoding sequences, preferentially (UG)n sequence, and the position of the TDP-43 binding site is associated with exclusion or inclusion of an exon [19,20]. We have shown that only a small number of the genes, which alter their splicing by depletion of TDP-43, are overlapped between HeLa and SH-SY5Y cells. The result indicates that the splicing regulation of TDP-43 is not simply resulted from the nucleotide sequence but also may be regulated by the interaction with other splice factors, including hnRNP A1, A2, and A2/B1 in each cell [10,11].
Although several studies have reported that the pre-mRNA splicing of genes, including CFTR, apoA-II and SMN2, was regulated by TDP-43 in vitro [8,28,29], our results show that depletion of TDP-43 does not affect the splicing for these genes. However, depletion of TDP-43 altered the pre-mRNA splicing in many genes as previously reported [19,20]. In contrast, relatively small numbers of genes were significantly altered in their amount of expression by depletion of TDP-43. Our results indicated that TDP-43 has relatively small effects in the regulation of the amounts of mRNA than the regulation of the splicing in culture cells.
A recent study revealed the alteration of splicing in the frontal cortex with frontotemporal lobar degeneration with TDP-43positive inclusions (FTLD-TDP), which is another type of TDP-43 proteinopathy [30]. They found an alteration of splicing for BIM (Bcl-2 interacting mediator of cell death) but not for POLDIP3 in brain from FTLD-TDP. The reason why the alteration of splicing for POLDIP3 is not observed in their analysis might be related to the difference of analysed region, frontal cortex and motor cortex or spinal cord, or the difference of the residual TDP-43 function. Indeed the intracellular and intra central nervous system distribution of TDP-43-positive inclusions are different between FTLD-TDP and ALS [31,32].
POLDIP3 is a substrate for S6K1, which is downstream of mTOR, and enhances the mTOR/S6K1-dependent translation of mRNA, which is associated with cell size [23,25]. Although it has been shown that the POLDIP3 variant-2 locates in the nucleus as well as variant-1 and retains the functional motif, the function of POLDIP3 variant-2 has not been well elucidated [21,25]. Most recently, it has been reported that the POLDIP3 variant-2 enhanced the mTOR/S6K1-dependent translation of mRNA more, and the depletion of TDP-43 increased cell size in HEK293E cells [26]. The results of our cell size assay in nonneuronal cell lines were consistent with this report. However, we have shown that the depletion of TDP-43 decreased cell size in the SH-SY5Y neuronal cell line. The difference for the effect of the depletion of TDP-43 on cell size could be explained by the cell type difference, neuronal or non-neuronal cell line. In addition, by the rescue experiment for cell size, we showed that POLDIP3 variant-2 is less effective than POLDIP3 variant-1 in regard to improving the size of SH-SY5Y cells reduced by the depletion of TDP-43 (Fig. 5E). Thus, the loss of function of TDP-43 may induce decreased function of POLDIP3 due to alteration of POLDIP3 splicing, which could result in down-regulation of mTOR/S6K1-dependent translation of mRNA in the neuronal cell line. Although loss of TDP-43 function affects the expression and the splicing of various genes, it would be interesting to investigate the contribution of the alteration of POLDIP3 splicing to the pathogenesis of ALS.
In conclusion, we have shown that the amounts of POLDIP3 variant-2 mRNA increased in affected tissues with ALS, indicating the loss of function of TDP-43 in ALS. In addition, increasing the amount of POLDIP3 variant-2 has a potential to be an ideal and sensitive biomarker to represent the dysfunction of TDP-43, since the POLDIP3 variant-2 is rarely expressed in normal tissues. Thus, the development of the method to detect the amounts of POLDIP3 variant-2 in cerebrospinal fluid would be interesting to diagnosis and evaluate the progression of ALS.

Subjects
Details of the patients and controls included in this study are compiled in Table S1. This study was approved by the Institutional Review Board of Niigata University, and written informed consent was obtained from families.

Exon array analysis
The Affymetrix GeneChip Whole Transcript Sense Target Labeling Assay was used to generate amplified and biotinylated sense-strand DNA targets (Affymetrix, Santa Clara, CA, USA). Probe was hybridized to Affymetrix 1.0 Human Exon ST arrays according to the manufacturer's instructions (Affymetrix). This array is able to determine the difference at both exon-and genelevel expressions. The arrays were scanned with an Affymetrix Gene Chip scanner 3000 system (Affymetrix). Initial data were processed to CEL file using GeneChip Operating Software (Affymetrix). Analysis of microarray data was performed with Genespring GX ver.11 (Agilent Technologies, Palo Alto, CA, USA). CEL files were imported into the software and their backgrounds underwent Robust Multichip Average and quantile by real-time qRT-PCR using the primers indicated with arrows in Fig. 2A. In this experiment, RPLP1 and RPS18 were used as reference genes. Data represent the mean with standard error from three independent experiments. Asterisk indicates significant difference among TDP-43 39-UTR siRNAtransfected cells (*P,0.01, Tukey multiple comparison test). doi:10.1371/journal.pone.0043120.g003 Total RNA was isolated from the thalamus, motor cortex, and spinal cord of controls (n = 527) and patients with ALS (n = 627) and qRT-PCR analysis was performed using the primers indicated with arrows in Fig. 2A. POLDIP3 mRNA of total, variant-1, and variant-2 are represented as a fold change relative to controls. In this experiment, RPLP1 and RPS18 were used as reference genes. Data represent the mean with standard error. Asterisk indicates significant difference (*P,0.05, Student t test). (B) Purity of the motor neuron enriched material obtained by laser microdissection methods. We isolated 100 motor neurons from L2 in each individual control (n = 4) or individual with ALS (n = 4). Real-time qRT-PCR revealed that Tthe expression level of ChAT mRNA, a motor neuron marker, was only detected in the motor neuron-enriched material. In contrast, similar expression levels of RPLP1 mRNA, which was one of the internal controls used in this experiment, were detected in both materials. (C) Increased POLDIP3 variant-2 in ALS motor neurons. POLDIP3 variant-1 and variant-2 mRNA levels as a fold change relative to those of control are presented by a scatter plot. In this experiment, RPLP1 and RPS18 were used as reference genes. Solid line represents the mean with standard error. Asterisk indicates significant difference (*P,0.05, Student t test). doi:10.1371/journal.pone.0043120.g004 TDP-43 Related Splicing Alteration in ALS Tissues PLOS ONE | www.plosone.org normalizations. For our analysis, we have used only the core probe set. Statistical analysis was performed by creating two groups from the individual CEL files, transfection with control siRNA or with TDP-43 siRNA, and then comparing them. From these array data, we calculated the P value the splicing index (SI), and fold change. Both a P,0.05 and an |SI|.0.5 were used as thresholds to identify the aberrant spliced genes, and both a P,0.05 and a fold change 2.0 were used for determining differentially expressed genes.

Real-time quantitative RT-PCR analysis
Total RNA was isolated from siRNA transfected cells with the RNeasy plus mini-kit (Qiagen, Valencia, CA, USA). cDNA was synthesized with a high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA). For autopsied human frozen tissues, mirVana miRNA isolation kit was used to isolate total RNA (Applied Biosystems), followed by synthesis of cDNA using SuperScriptH VILOTM cDNA Synthesis Kit (Invitrogen). The RNA quality was assessed by the RNA integrity number (RIN) of the Agilent Bioanalyzer 2100 (Agilent Technologies). RIN score in each sample were described in Table S1. Real-time qRT-PCR was conducted with SYBR Premix Ex Taq on the Thermal Cycler Dice (Takara, Shiga, Japan). Thermal cycler setting was 1) 95uC, 30 sec, 2) 95uC, 15 sec, 3) 60uC, 30 sec and repeat 40 cycles. In each qRT-PCR experiments, Ribosomal protein, large P1 (RPLP1) and ribosomal protein S18 (RPS18) were used as reference genes, which are determined by geNorm [33]. We designed primer pairs for POLDIP3 variant-1 and  protein was immunoprecipitated with anti-TDP-43 antibody (ProteinTech Group) from HeLa cell lysates, and the RNAs isolated from the immunoprecipitated material were subjected to RT-PCR for the POLDIP3 transcripts. Control rabbit IgG was used as a negative control (Abcam, Cambridge, MA, USA). The primer pairs for POLDIP3 are as follows; POLDIP3 RNA-IP F: 59-GCTAAGCAGTTCCAAGCTTTCC-39, POLDIP3 RNA-IP R: 59-TGGCAGCTCTTTGGGGGGTT-39.

Cell size assay
For determining the cells in G 1 phase, we stained the cells with 7-amino-actinomycin D (7-AAD) according to the manufacturer's instructions (BD Biosciences, San Jose, CA). Briefly, cells were fixed and permeabilized with ice cold 80% methanol and incubated for 1 minute on ice. After washing twice with phosphate-buffered saline, cells were incubated with 7-AAD (SIGMA) for 15 minutes at room temperature in the dark. DNA content was determined by FACSCalibur TM (BD Biosciences) and data were analyzed using CellQuest Pro software (BD Biosciences). The cells with G 0 /G 1 phase DNA content were analyzed to determine the cell size.
To further elucidate the functional difference between POL-DIP3 variant-1 and POLDIP3 variant-2, we attempted to investigate cell size following treatment with TDP-43 siRNA in SH-SY5Y cells that stably expresses GFP-tagged POLDIP3 variant-1 or variant-2. To estimate the recovery rate of cell size correctly, we used GFP-negative cells in each population as controls. We transfected the cells with TDP-43 or control siRNAs, followed by measuring cell size in each population by FACSCalibur TM (BD Biosciences) and analyzing data by using CellQuest Pro software (BD Biosciences). From the measured value of cell size, we calculated the recovery rate of cell size by comparing the measured value between the cells with or without expression of GFP-tagged POLDIP3 variant. The equation for calculating the recovery rate is as follows: Recovery rate of cellsize % ð Þ~TC=CC{TV=CV ð Þ = TC=CC{1 ð Þ |100 TC = forward scatter of control cells treated with TDP-43 siRNA, CC = forward scatter of control cells treated with control siRNA, TV = forward scatter of cells expressed GFP-tagged POLDIP3 variant treated with TDP-43 siRNA, and CV = forward scatter of cells expressed GFP-tagged POLDIP3 variant treated with control siRNA. The schematic diagram of the cell size rescue experiment is also shown in Fig. S4. Figure S1 qRT-PCR analysis for validation of exon array in other 4 genes. (A) Exon structure diagram of the main isoform (top) and candidate variant (bottom) for MMAB, GLS, STRA6, DPM2. From the exon array result, we selected the candidate splicing variant that was induced by the depletion of TDP-43. The name of each transcript is labeled on the side of the exon structure. The red box indicates the exons that are expected to be altered by TDP-43 depletion. The black bars indicate the position of the primers we used in this experiment. The gene views show the expression of exons as determined by analyzing the results of exon array in HeLa cells using Genespring GX. The expression levels are shown on a log2 scale; the error bars show standard errors of means. TDP-43 siRNA, red circle; control siRNA, blue square. (B) qRT-PCR analysis revealed that the splicing alteration of these genes was not validated. RPLP1 and RPS18 were used as reference genes. Data represent the mean with standard error from three independent experiments. Asterisk indicates significant difference (*P,0.01, Student t test). (TIF) Figure S2 Immunohistochemical analysis for TDP-43 in the thalamus. To investigate the presence of TDP-43 pathology in the thalamus from ALS cases used in Figure 4A, TDP-43 immunostaining was performed. Four out of six ALS cases showed (A) glial, and (B-D) neuronal cytoplasmic TDP-43 immunoreactivity. The label on each figure corresponds to the ID described in Table S1. Scale bar, 20 mm.