Anti-IL-2 Treatment Impairs the Expansion of Treg Cell Population during Acute Malaria and Enhances the Th1 Cell Response at the Chronic Disease

Plasmodium chabaudi infection induces a rapid and intense splenic CD4+ T cell response that contributes to both disease pathogenesis and the control of acute parasitemia. The subsequent development of clinical immunity to disease occurs concomitantly with the persistence of low levels of chronic parasitemia. The suppressive activity of regulatory T (Treg) cells has been implicated in both development of clinical immunity and parasite persistence. To evaluate whether IL-2 is required to induce and to sustain the suppressive activity of Treg cells in malaria, we examined in detail the effects of anti-IL-2 treatment with JES6-1 monoclonal antibody (mAb) on the splenic CD4+ T cell response during acute and chronic P. chabaudi AS infection in C57BL/6 mice. JES6-1 treatment on days 0, 2 and 4 of infection partially inhibits the expansion of the CD4+CD25+Foxp3+ cell population during acute malaria. Despite the concomitant secretion of IL-2 and expression of high affinity IL-2 receptor by large CD4+ T cells, JES6-1 treatment does not impair effector CD4+ T cell activation and IFN-γ production. However, at the chronic phase of the disease, an enhancement of cellular and humoral responses occurs in JES6-1-treated mice, with increased production of TNF-α and parasite-specific IgG2a antibodies. Furthermore, JES6-1 mAb completely blocked the in vitro proliferation of CD4+ T cells from non-treated chronic mice, while it further increased the response of CD4+ T cells from JES6-1-treated chronic mice. We conclude that JES6-1 treatment impairs the expansion of Treg cell population during early P. chabaudi malaria and enhances the Th1 cell response in the late phase of the disease.


Introduction
The asexual blood stages of the Plasmodium ssp. are responsible for the pathology and morbidity caused by malaria, an infectious disease that remains a major devastating illness afflicting 350 to 500 million people annually and resulting in more than 1 million deaths per year [1]. Among the cell populations involved in the immune response to the blood stages of malaria, effector Th1 cells are thought to play a key role in both disease protection and pathogenesis [2,3,4]. Thus, an appropriate regulatory balance between protective immune responses and immune mediated pathology is required for a favorable outcome of infection [5]. The suppressive activity of regulatory T (T reg ) cells has been implicated in the development of clinical immunity to disease known as premunition, which occurs concomitantly with persistence of low parasite burdens rather than sterilizing immunity [5]. However, despite their relevance, the molecular pathways required to induce and to sustain the suppressive activity of T reg cells in malaria are still poorly characterized.
In the blood stage malaria caused by the rodent parasite, Plasmodium chabaudi, the spleen CD4 + T cell population expands quickly, secretes considerable amounts of interferon-c (IFN-c) and helps B cells to produce high quantities of polyclonal immunoglobulin (Ig) M and IgG2a [6,7,8]. Both IFN-c [9] and acute phase antibodies [10] have been implicated in the initial control of the parasite. Effector Th1 cells also contribute to pathogenesis in acute P. chabaudi malaria because mice lacking IFN-c or deprived of this cell population have attenuated symptoms [11]. As the disease progresses, the majority of lymphocytes activated during early infection are eliminated by apoptosis [12], giving the opportunity to the development of a large pool of effector-memory CD4 + T cells that cooperate with B cells in the production of parasite-specific high-affinity antibodies and have the capacity to secrete IFN-c upon stimulation [13]. Similar to humans infected with Plasmodium falciparum, the development clinical immunity to P. chabaudi malaria occurs simultaneously with persistence of low levels of chronic parasitemia [14], and T reg cells have also been implicated in both processes [5]. The cooperation between high-affinity parasite-specific IgG and memory Th1 cells is required for complete parasite clearance after 2-3 months of infection and also for acquisition of full protective immunity against reinfection [14,15].
In contrast to the many studies addressing the role of CD4 + T cells in protection against P. chabaudi malaria, little is known about the molecular mechanisms responsible for CD4 + T cell proliferation, differentiation and regulation. IL-2 has multiple and opposing activities contributing to both the induction and the control of immune responses [16,17]. Both activated and regulatory CD4 + T cells express CD25, the a chain of the high-affinity IL-2 receptor (IL-2R) that combines with the IL-2R b chain (CD122) and the common c chain (cc or CD132). While activated CD4 + T cells can produce their own IL-2, T reg cells depend on paracrine IL-2 for their generation and maintenance and for the exertion of their suppressive functions [18]. Thus, although IL-2 was first identified as a potent T cell growth factor [19] that also displays pro-apoptotic activity [20], the main non-redundant activity of IL-2 is to promote T cell tolerance and homeostasis [21,22]. Moreover, IL-2 is required for effector Th1 and Th2 cell differentiation, provides a competitive advantage to T cells, resulting in optimal survival and performance of memory cells, and inhibits the development of inflammatory Th17 cells [16].
In the present study, we analyzed in detail the effects of anti-IL-2 treatment with JES6-1 monoclonal antibody (JES6-1 mAb) on the CD4 + T cell response to P. chabaudi. This mAb has been shown to block the CD8 + T cell response to IL-2 in vitro via the lowaffinity IL-2R bc, apparently for biding to an IL-2 site that is crucial for interaction with CD122 but less crucial for binding to CD25 (high-affinity IL-2Rabc) [23]. Because IL-2 bound to JES6-1 mAb has extended in vivo half-time and retains the ability to interact with the high-affinity IL-2R, injection of a premixed 2:1 molar ratio of IL-2/JES6-1 mAb complexes has been used to potentiate IL-2 signaling and induce expansion of the T reg cell population [24]. Thus, analyzing the effects of JES6-1 treatment on P. chabaudi malaria contributes to the efforts to understand the molecular mechanisms responsible for activation and regulation of the CD4 + T cell response to Plasmodium aiming to ameliorate the outcome of the disease.

Results
Expression of IL-2R a (CD25) and b (CD122) chains in the splenic CD4 + T cell response to P. chabaudi malaria The infection of C57BL/6 mice with 10 6 parasitized red blood cells (PRBC) resulted in a rapid increase in splenic CD4 + T cell numbers, attaining maximum levels concomitant with the peak of parasitemia on day 7 post-infection (p.i.) (Fig. 1A). The increase of the CD4 + T cell population was accompanied by intense proliferation and culminated in a prominent but short-lasting peak of IFN-c production (Fig. 1B). As recently shown, the great majority of proliferating and IFN-c-producing CD4 + cells in the spleen during the early infection are class II MHC-restricted CD4 + T cells and not NKT cells [13]. On day 10 p.i., in parallel with the control of parasitemia, CD4 + T cell numbers per spleen abruptly decreased to values lower than those of non-infected mice (Fig. 1A). The normalization of this population occurred concomitantly with a second wave of CD4 + T cell proliferation that peaked on day 20 p.i. and decreased thereafter (Fig. 1B).
Along with the proliferative response to infection, there was a notable increase in CD122 expression, which gave evidence of two different CD4 + T cell subsets (Fig. 1C). The CD25 + CD122 + subset reached its maximum on days 4 and 7 p.i. before decreasing. The CD25 2 CD122 + subset appeared later, being first observed on day 7 p.i. and still detectable on days 15 p.i. (data not shown) and 30 p.i. when it corresponded to 13.6% and 8.1% of CD4 + T cells, respectively. On day 7 p.i., both CD4 + CD122 + cell populations had a large size, whereas the majority of CD4 + CD25 + CD122 2 cells were smaller (Fig. 1D). CD4 + CD25 + CD122 + cells from 4-day infected mice and CD4 + CD25 2 CD122 + cells from 15-day infected mice were also large, whereas CD4 + CD25 + CD122 2 cells from 30-day infected mice were smaller (data not shown). As expected, CD4 + CD25 + CD122 LO/cells, a phenotype characteristic of T reg cells, were also observed in non-infected mice. These results show that splenic CD4 + T cells that respond to acute P. chabaudi malaria express the IL-2R b chain, but only a subset co-express the a chain. During chronic infection, the majority of activated CD4 + T cells present only the IL-2R b chain.
Secretion of IL-2 and expression of activation markers by splenic CD4 + T cells during P. chabaudi malaria Concomitantly with the high expression of IL-2R, the percentages of IL-2-secreting CD4 + T cells progressively increased from day 3 to 7 p.i., decreasing to levels similar to those of noninfected mice on day 15 p.i. (Fig. 2A). IL-2-secreting CD4 + T cells were large in size and expressed high amounts of CD25 and CD122 (Fig. 2B). The majority of large CD4 + CD25 + cells in 7day infected mice produced IL-2 ( Fig. 2C). Small CD4 + CD25 + cells had a phenotype typical of T reg cells in both groups of mice. When compared to CD4 + CD25 2 cells, these cells expressed significantly higher levels of mTGF-b, CTLA-4 and GITR, slightly higher levels of CD122 and lower levels of CD45RB. In contrast, large CD4 + CD25 + cells had a phenotype characteristic of activated cells, differing from small CD4 + CD25 + cells by the extremely high expression of CD122, CD45RB and CTLA-4. These results show that IL-2 is produced during acute P. chabaudi malaria by large CD4 + T cells that express high levels of IL-2R, together with several other activation markers.
Effects of JES6-1 treatment on the early CD4 + T cell response to P. chabaudi malaria C57BL/6 mice treated with JES6-1 monoclonal antibodies (mAb) on days 0, 2 and 4 p.i. with 10 6 PRBC were analyzed according to expression of IL-2Ra and b chains and the activation markers CD69 (early activation-induced C-type lectin) and CD44 (cell-cell contact adhesion molecule). The production of IL-17 was also evaluated because anti-IL-2 treatment could shift the immune response towards the Th17 profile, due to the negative control exerted by IL-2 over development of inflammatory Th17 cells [16]. Based on the percentages of CD4 + cells showing high expression of CD122, CD25, CD69 and CD44 and large size, JES6-1 mAb had negligible effects on CD4 + T cell activation with only a minor inhibition of CD25 expression (Fig. 3A). The cytokine pattern of CD4 + T cells responding to acute infection remained unchanged where the production of IFN-c predominated over IL-17 secretion (Fig. 3B). Nevertheless, JES6-1 treatment resulted in a significant increase in basal (non-stimulated) IFN-c production by CD4 + T cells in both non-infected mice and 7-day infected mice. The CD4 + T cell numbers per spleen (Fig. 3C) and the capacity to control parasitemia (data not shown) were not affected by JES6-1 treatment. Therefore, we also evaluated the in vitro effects of the JES6-1 mAb on PRBCstimulated CD4 + T cell proliferation. Cells obtained on day 4 p.i. were analyzed because this is the peak of the in vitro proliferative response to PRBC during acute infection [13]. As observed in vivo, the JES6-1 mAb had no inhibitory effect on in vitro CD4 + T cell proliferation (Fig. 3D). These results show that JES6-1 treatment does not significantly alter the CD4 + T cell activation, proliferation and IFN-c production during acute P. chabaudi malaria.
Effects of JES6-1 treatment on the CD4 + CD25 + FoxP3 + cell population during P. chabaudi malaria The expression of FoxP3 was evaluated in splenic CD4 + CD25 + cells from C57BL/6 mice on days 7 and 20 p.i. with 10 6 PRBC. In non-infected mice, the great majority of CD4 + CD25 + cells expressed FoxP3, a phenotype characteristic of T reg cells (Fig. 4A). On day 7 p.i., although the majority of large cells were comprised in the CD4 + CD25 + FoxP3 2 subpopulation, there was an increase of 69.3% in the large CD4 + CD25 + FoxP3 + subpopulation. When considering cell number per spleen, there was a 3-fold increase in the CD4 + CD25 + FoxP3 + cell population on day 7 p.i. followed by a reduction of 40.7% by day 20 p.i. (Fig. 4B). JES6-1 treatment partially reduced CD4 + CD25 + FoxP3 + cell numbers per spleen in non-infected and 7-day infected mice. The involvement of IL-2 in the maintenance/expansion of the T reg cell population was also evidenced by data showing a significant decrease in CD4 + CD25 + FoxP3 + cell percentages in non-infected and 7-day infected mice treated with JES6-1 mAb compared to non-treated controls.
Effects of JES6-1 treatment on CD4 + T cell phenotype and proliferative response during chronic P. chabaudi malaria Because IL-2 secreted during acute P. chabaudi malaria might influence the late phase of the immune response either directly or through its effects on T reg cells, we evaluated the effects of JES6-1 treatment on the CD4 + T cell phenotype and proliferative response on days 20 and 30 p.i., respectively. These time points coincided with the peak and the end of the second wave of CD4 + T cell proliferation, respectively (Fig. 1B). Twenty days after the beginning of JES6-1 treatment, there was no significant difference in CD4 + T cell numbers per spleen between non-infected mice and infected mice (data not shown). However, JES6-1 treatment resulted in additive increases in percentages of CD4 + CD62L LO CD45RB LO (effector-memory) cells and CD4 + CD69 + (activated) cells ( Fig. 5A and 5B). Mice treated with anti-bgalactosidase isotype control (GL117 mAb) did not differ from non-treated mice (data not shown). Non-stimulated (basal) and PRBC-stimulated CD4 + T cell proliferation was also increased in JES6-1-treated mice, a phenomenon observed in non-infected and infected mice (Fig. 5C). The CD8 + T cell proliferation in response to PRBC was also enhanced in both groups of JES6-1-treated mice (3.760.1% and 13.061.6% of CSFE LO cells, p,0.05) compared to non-treated mice (1.96 0.1% and 4.860.4% of CSFE LO cells). Moreover, JES6-1 mAb completely blocked the PRBC-stimulated proliferation of CD4 + T cells from non-treated mice, while it further increased the response of CD4 + T cells from JES6-1-treated mice. These results show that JES6-1 treatment enhances the CD4 + T cell response during the chronic phase of P. chabaudi infection.
Effects of JES6-1 treatment on the development of protective immunity to P. chabaudi malaria Because CD4 + T cells developed in JES6-1-treated mice have an improved capacity to proliferate when stimulated with PRBC, we sought to verify the effects of in vivo JES6-1 treatment on the production of proinflammatory cytokines and parasite-specific IgG2a. In general, JES6-1 treatment increased the non-stimulated (basal) and PRBC-stimulated TNF-a and IFN-c production in both non-infected and 30-day infected mice (Fig. 6A). However, there was no effect on PRBC-stimulated TNF-a secretion by spleen cells from non-infected mice and PRBC-stimulated IFN-c secretion by spleen cells from 30-day infected mice. On day 30 p.i., the serum titers of parasite-specific IgG2a were significantly higher in JES6-1-treated mice compared to non-treated mice (Fig. 6B). Interestingly, similar levels of PRBC-stimulated TNF-a and IFN-c production and parasite-specific IgG2a were found in non-treated 30-day infected mice and in JES6-1-treated non-infected mice. Both JES6-1-treated and non-treated infected mice efficiently controlled a secondary challenge with 10 8 PRBC given on day 30 p.i. (data not shown). Additionally, mice injected with spleen cells from 30-day infected mice showed lower parasitemia peaks following infection when cells were taken from JES6-1-treated mice (Fig. 6C). We concluded that JES6-1 treatment enhances the Th1 cell response, leading to increased production of TNF-a and parasite-specific IgG2a antibodies and optimizing the protective immunity to P. chabaudi malaria.

Discussion
We have recently proposed that the splenic CD4 + T cell response to blood stages of P. chabaudi malaria develops in two consecutive phases where conventional CD4 + T cells are the main protagonists [13]. The early phase of the response may establish a bridge between innate and acquired immunity, as it rapidly provides large amounts of proinflammatory cytokines and help B cells to secrete polyclonal Ig. The late phase of the response generates a large pool of effector-memory CD4 + T cells that cooperate with B cells in the production of parasite-specific high-affinity antibodies and have the capacity to secrete IFN-c upon stimulation. In the present study, we examined in detail the effects of JES6-1 treatment on the early and late phases of CD4 + T cell response to P. chabaudi infection.
Initially, we observed that IL-2 is produced during acute infection by activated CD4 + T cells expressing high levels of IL-2R a and b chains, as well as the activation markers GITR, mTGF-b, CTLA-4 and CD45RB. The consumption of IL-2 by activated lymphocytes could explain the low levels of this cytokine detected in culture supernatants from human and mouse with acute malaria [8,25], an interference minimized in our analysis by using a bifunctional anti-IL-2 mAb that binds to the cell surface and competes with IL-2R for capturing the cytokine molecules soon after their release into the extracellular milieu. Corroborating our data, increased production of IL-2 was observed by intracellular staining in blood CD4 + T cells from acute malaria patients [26]. Moreover, intracellular IL-2 production coincides with increase of T reg cell population in the spleen during P. chabaudi malaria [27].
The concomitant production of IL-2 and expression of high affinity IL-2R by activated CD4 + T cells during acute infection implicates this cytokine as a participant in the immune response to P. chabaudi malaria. The lack of effect of in vivo or in vitro JES6-1 treatment in the early CD4 + T cell activation, proliferation and IFN-c production, and consequently in the ability to control acute parasitemia, can be explained by the high expression of CD25 since IL-2 bound to JES6-1 mAb retains the ability to interact with the high-affinity IL-2R [23]. However, it is also possible that the major role of IL-2 during the early CD4 + T cell response to P. chabaudi infection is to expand the T reg cell population, while the effector CD4 + T cell response is independent of IL-2. These possibility is supported by data showing that IL-2 is not required for the initial cycling of T cells, as suggested by previous studies showing that T cell proliferation is not abrogated by anti-IL-2 or anti-IL-2R mAb after strong stimulation through the T cell receptor (TCR) [28,29]. Moreover, several studies using IL-2-or IL-2R-deficient mice clearly indicate that T cell activation and proliferation occur in vitro and in vivo independently of all ccdependent cytokines but are dependent on TCR and costimulatory signals [30,31,32,33].
In agreement with this notion, it has been previously shown that T cell immunity to P. chabaudi [34], as well as to other infectious pathogens [35], can be generated in the absence of IL-2. Thus, it is reasonable that intense costimulation makes IL-2 dispensable for T cell activation, a suitable possibility if we consider that IL-2R and the CD28, ICOS and OX40 costimulatory receptors relay intracellular signals through phosphoinositide 3-kinases (PI3K), which culminate in a broad variety of cell biological effects, including proliferation and cytokine synthesis [36]. Accordingly, unpublished data from our laboratory revealed the high expression of ICOS and OX40 during the early CD4 + T cell response to P. chabaudi malaria (Castillo-Méndez et al., manuscript in preparation). The abundance of costimulatory signals could also cooperate to inactivate T reg cells [37] and consequently enlarge the spectrum of CD4 + T cell specificities involved in the early response to infection.
The idea that T reg cell inactivation contributes to the early CD4 + T cell response to infection is indirectly supported by our data showing a massive response to parasites in non-infected mice a month after JES6-1 treatment, a condition where T reg cells may have been inactivated by IL-2 deprivation. This increased CD4 + T cell response to parasites in JES6-1-treated non-infected mice occurs in terms of both PRBC-stimulated proliferation and IFN-c production. The mechanism triggering robust CD4 + T cell response to PRBC a month after JES6-1 treatment of noninfected mice is currently been investigated by our research group. Our working hypothesis is that depletion of T reg cell population due to IL-2 inhibition results in a long-lasting deficiency in the ability to regulate the immune system, leading to CD4 + T cell hyperresponsiveness to PRBC. Although the number of T reg cells per spleen was reestablished on day 20 p.i., the reduced expression of regulatory molecules, such as CTLA-4 and PD-1, may impair their suppressive activity.
The increase in T reg cell population has been previously reported in P. chabaudi and Plasmodium yoelii infections [38,39], but its implication in the control of immune responses to malaria is still a matter of debate [5]. Our data extend these findings by showing that IL-2 is partially required for expanding the CD4 +   CD25 + FoxP3 + cell population during P. chabaudi malaria. The fact that some increase in CD4 + CD25 + FoxP3 + cell numbers per spleen still occurred in acutely infected JES6-1-treated mice could also be related to the abundance of costimulatory signals, which have been implicated in T reg cell development and peripheral homeostasis [40]. It is worth noting that, in our analysis, the impaired increase in CD4 + CD25 + FoxP3 + cell numbers due to JES6-1 treatment does not significantly affect the early CD4 + T cell response to parasites, as the only difference observed after JES6-1 treatment in acutely infected mice is a slight augmentation of IFN-c production.
The effects of JES6-1 treatment in vitro drastically change during the infection in which inhibition of CD4 + T cell response to parasites is observed at the chronic phase of the disease, but not at the acute phase as discussed above. The results showing the ability of JES6-1 mAb to block in vitro CD4 + T cell proliferation at the chronic infection demonstrate the effectiveness of this treatment to completely inhibit IL-2 signaling. This shift could be a consequence of the reduction of PI3K-mediated costimulatory signals leading to IL-2 dependency. This process could involve IL-2 induced expression of regulatory molecules, such as PD-1 and CTLA-4, in CD4 + T cells [41,42]. This would increase the threshold for TCR signaling and, as a consequence, restrain the spectrum of CD4 + T cell specificities involved in the response and provide an IL-2 driven competitive advantage to parasite-specific CD4 + T cells [43]. IL-2 dependency may also allow the control of self (cross) reactive clones by T reg cells, which are thought to operate by scavenging this cytokine [18,44].
The expansion of T reg cell population during the early CD4 + T cell response to infection may be essential for the development of a cohort of T reg cells shaped to modulate the pool of effectormemory and memory CD4 + T cells generated during the late phase of the response, by skewing their specificity repertoire towards the recognition of parasite-specific peptides and avoiding the expansion of self (cross) reactive clones. The T reg cell population was still increased on day 20 p.i. indicating that proliferating T reg cells were not eliminated after acute infection. T reg cell inactivation during the acute infection may explain the enhancement of cellular and humoral responses in JES6-1-treated mice during the chronic infection, which results in the improved capacity of spleen cells to transfer protection to naïve mice. Spleen cells from JES6-1-treated mice conferred a substantial protection (,40% reduction in parasitemia) if we consider the high parasite inoculum used in mouse challenge, and we can envisage that concomitant treatment with additional immunotherapies such as anti-IL-10 mAb could further improve parasite control.
Another possible explanation for the increase of immune response in JES6-1-treated chronic mice is related to the fact that, in some particular conditions, IL-2/JES6-1 mAb complexes can potentiate IL-2 signaling and amplify the effector T cell function [24]. Although this phenomenon is observed following administration of premixed 2:1 molar ratio of IL-2/JES6-1 mAb complexes, the possibility that similar conditions occur in vivo cannot be discarded. It should be noted, however, that in our experiments JES6-1 treatment results in substantial CD4 + T cell activation in non-infected mice, whereas IL-2/JES6-1 treatment leads to expansion of the T reg cell population and, in consequence, to reduction of CD4 + and CD8 + T cell responses to Plasmodium berghei infection [24] and to increase in parasitemia during P. chabaudi infection [27]. These opposite effects indicate that JES6-1 mAb is responsible for very different outcomes when administered alone or coupled with two molecules of IL-2, an artificial condition that may cause the approximation of two IL-2Ra chains and result in cell signaling independent of the b chain.
This work reinforces the idea that the CD4 + T cell response to P. chabaudi malaria develops in two distinct phases wherein JES6-1 treatment impairs the expansion of T reg cell population at the early phase and enhances the Th1 cell response at the late phase. This study may help to understand the molecular mechanisms involved in the immune response to Plasmodium and contribute to efforts aiming to manipulate this response to ameliorate the outcome of the disease.

Mice, parasites and infection
Six to eight-week-old C57BL/6 female mice were bred under pathogen-free conditions at the Isogenic Mice Facility, ICB-USP, São Paulo, Brazil. Plasmodium chabaudi AS was maintained as previously described [9]. Mice were infected intraperitoneally and parasitemias were determined by microscopic examination of Giemsa stained blood smears.

Ethics Statements
All procedures were in accordance with national regulations of ethical guidelines for mice experimentation and welfare of the conselho nacional de saúde and colégio brasileiro em experi- mentação animal (cobea), brazil, the protocols being approved by the health animal comitte (comissão de ética no uso de animaisceua -icb/usp) of the instituto de ciências biomédicas of the universidade de são paulo, são paulo, brazil, qith permit number 0019/2005 and 0036/2007.

Intracellular staining of Foxp3
Spleen cells (1610 6 ) were stained with anti-CD4 mAb and fixed with Cytofix/Cytoperm (BD Bioscience) for 30 min at room temperature. Cells were permeabilized with 1% formaldehyde for 30 min, centrifuged and washed twice in PBS containing 5% FCS and 0.1% NaN 3 . After incubation for 2 h with FITC conjugated anti-mouse/rat Foxp3 mAb, cells were washed and analyzed by flow cytometry.

IL-2 secretion assay
The IL-2 PE cytokine secretion assay was performed according to the manufacturer's recommendations (BD Biosciences). This assay uses a bi-functional mAb capable of binding CD45 on one arm and IL-2 on the other arm. Spleen cells (2610 6 ) were incubated with the bi-functional mAb for 45 min at 37uC in 5% CO 2 . The presence of IL-2 bound to bi-functional mAb was detected with PE-labeled anti-IL-2 mAb. As a positive control, spleen cells (2610 6 ) from non-infected mice were cultured in vitro for 3 h with anti-CD3 mAb (BD Pharmingen). The analyses were performed by flow cytometry.

Multicytokine assessment
IFN-c and TNF-a were quantified simultaneously by a cytometric bead array (CBA; BD Pharmingen) of supernatants obtained from the same cultures used in the proliferation assay. This technique uses flow cytometry to measure soluble analytes in a particle-based immunoassay and was carried out according to the manufacturer's instructions (BD Pharmingen). The lower limit of detection for all cytokines in this assay was 20 pg/ml.

Intracellular staining for IFN-c and IL-17
For intracellular IFN-c and IL-17, spleen cells (1610 6 ) were cultured with GolgiStop overnight at 37uC in 5% CO 2 according to the manufacturer's instructions (BD Pharmingen). After washing, cells were surface stained with FITC-or Cy-Chromelabeled mAb to CD4 and CD8. Cells were then fixed with Cytofix/Cytoperm buffer and incubated with PE-labeled mAb to IFN-c and IL-17 diluted in Perm/Wash buffer. The analysis was performed by flow cytometry.

Parasite-specific ELISA
Anti-P. chabaudi antibodies were quantified by ELISA as previously described [46]. In brief, 96-well flat-bottom microtiter plates (Costar) were coated overnight (4uC) with a total parasite extract (8 mg/ml). Plates were saturated with 1% BSA (bovine serum albumin) for 1 h. After washing, wells were incubated with 100 ml mouse serum (diluted from 1:50 to 1:6400) for 90 min at room temperature. Assays were developed with goat anti-mouse IgM or IgG2a peroxidase conjugated antibodies (Southern Biotechnology Associates) for 1 h, followed by 100 ml/well of TMB (tetra-methyl-benzidine) (Zymed) for 15 min, and the absorbance was quantified with a Spectra Max 190 spectrophotometer (Molecular Devices) at 650 nm. The antibody level in each serum sample was expressed as the reciprocal of the endpoint titer, which was defined in serial dilutions as the lowest dilution with a background optical density (O. D.).

Statistical analysis
Statistical analysis was generally performed with t-test and Mann Whitney test using Graph Pad Prism 4 software. Differences between two groups were considered significant when the p value was ,0.05 (5%).