Universal Stress Proteins Are Important for Oxidative and Acid Stress Resistance and Growth of Listeria monocytogenes EGD-e In Vitro and In Vivo

Background Pathogenic bacteria maintain a multifaceted apparatus to resist damage caused by external stimuli. As part of this, the universal stress protein A (UspA) and its homologues, initially discovered in Escherichia coli K-12 were shown to possess an important role in stress resistance and growth in several bacterial species. Methods and Findings We conducted a study to assess the role of three homologous proteins containing the UspA domain in the facultative intracellular human pathogen Listeria monocytogenes under different stress conditions. The growth properties of three UspA deletion mutants (Δlmo0515, Δlmo1580 and Δlmo2673) were examined either following challenge with a sublethal concentration of hydrogen peroxide or under acidic conditions. We also examined their ability for intracellular survival within murine macrophages. Virulence and growth of usp mutants were further characterized in invertebrate and vertebrate infection models. Tolerance to acidic stress was clearly reduced in Δlmo1580 and Δlmo0515, while oxidative stress dramatically diminished growth in all mutants. Survival within macrophages was significantly decreased in Δlmo1580 and Δlmo2673 as compared to the wild-type strain. Viability of infected Galleria mellonella larvae was markedly higher when injected with Δlmo1580 or Δlmo2673 as compared to wild-type strain inoculation, indicating impaired virulence of bacteria lacking these usp genes. Finally, we observed severely restricted growth of all chromosomal deletion mutants in mice livers and spleens as compared to the load of wild-type bacteria following infection. Conclusion This work provides distinct evidence that universal stress proteins are strongly involved in listerial stress response and survival under both in vitro and in vivo growth conditions.


Introduction
Universal stress proteins (Usps) comprise a group of proteins induced by different stress conditions and are found in numerous prokaryotic as well as eukaryotic organisms [1,2].Among these, universal stress protein A (UspA) of Escherichia coli K-12 is best characterized and found to be highly expressed in response to heat, substrate starvation, exposure to antimicrobial agents and oxidative stress [1].
The majority of usp genes are monocistronically expressed, and different transcription factors, such as s 32 , s 70 and s E promote transcription of Usps [1].
The significance of Usps in the model pathogen Listeria monocytogenes is presently unknown.L. monocytogenes is a ubiquitously occuring grampositive, facultative intracellular bacterium that causes food-borne infections, which mainly affect pregnant women, newborns, elderly and immunocompromised patients [17,18].L. monocytogenes possesses the remarkable ability to grow under a wide range of temperatures, pH conditions and high osmolarity, allowing the pathogen to survive in nature, food preservation methods as well as in infected host cells [18].
Here we present first evidence that UspA domain containing stress proteins are of importance for L. monocytogenes to survive under different stress conditions both in vitro and in vivo.

Bacterial strains, plasmids and culture conditions
All bacterial strains and plasmids used in this study are listed in Table 1.
The wild type strain L. monocytogenes EGD-e serotype 1/2a [19] and its isogenic usp deletion mutants or complemented strains were cultivated aerobically in Brain Heart Infusion medium (BHI; Difco) at 37uC or on BHI agar plates at 37uC.E. coli strain (DH10b M ) was grown in Luria-Bertani broth or LB agar plates at 37uC.When required, antibiotics were added to the following concentrations: erythromycin (Sigma), 300 mg/ml for E. coli and 5 or 10 mg/ml for Listeria; ampicillin (Sigma), 50 mg/ml for E. coli and 200 mg/ml for Listeria.

Construction of chromosomal usp deletion mutants
Generation of the usp in frame deletion mutants was done as previously described [20][21][22].Briefly, the flanking regions of each usp gene were amplified by PCR (Taq polymerase; Fermentas) using primer pairs 1 and 2b for the 59 flanking region and 3b and 4 for the 39 flanking region (Table 2), respectively.The resulting PCR products were fused to each other by using primers 1 and 4 in a second PCR reaction with Taq polymerase and the product was cloned into pCR2.1-TOPOH.
For the generation of Dlmo0515 and Dlmo2673 the pCR2.1-TOPOHvector containing the flanking region of the respective gene was digested with BamHI, XhoI and NcoI (Fermentas) and inserted into pAUL-A [21], that was previously digested with BamHI and SalI (Fermentas).The pCR2.1-TOPOH vector containing the flanking region of Dlmo1580 was digested with XbaI and SacI (Fermentas) and ligated with pAUL-A, also previously digested with the same restriction endonucleases.
We designated resulting pAUL-A vectors containing the flanking regions of the respective usp genes, pCS1 for Dlmo0515, pCS2 for Dlmo1580 and pCS3 for Dlmo2673.These vectors were transformed into E. coli and were isolated, sequenced and subsequently electroporated into L. monocytogenes wild-type strain.Gene replacement was performed as previously described by Schaeferkordt et al. [21,22].To create a triple mutant (Dlmo0515Dlmo1580Dlmo2673) for all three usp genes, gene replacement were carried out as described above starting with the electroporation of pCS2 into Dlmo0515.Finally, pCS3 was electroporated into the resulting double mutant to generate the isogenic triple mutant (Dlmo0515Dl-mo1580Dlmo2673).The chromosomal deletion was confirmed by DNA sequencing of PCR products using primer 7 and 8 (Table 2).The complementation of the Dlmo0515, Dlmo1580 and Dlmo2673 deletion mutants was carried out using the L. monocytogenes site specific phage integration vector pPL2 [23].PCR products were generated using primer pairs 5 and 6 with Taq polymerase.The product was cloned into pCR 2.1-TOPOH.The pCR 2.1-TOPOH vector containing the usp gene region of lmo0515 or lmo2673 was digested with BamHI and XhoI (Fermentas) and for lmo1580 with XhoI and SacI (Fermentas), respectively.The insert was ligated with pPL2, also previously digested with the same restriction endonucleases.Primer sequences used for the complementation of the deleted genes are listed in table 2.

Acid stress assay
Single colonies of the wild type L. monocytogenes and its usp deletion mutants grown on BHI agar plates were used to inoculate 100 ml Erlenmeyer flasks containing 10 ml of BHI broth, followed by overnight incubation at 37uC with shaking (180 rpm, Unitron Infors).The overnight cultures were then subcultured (1:50) in 40 ml of BHI and grown to an optical density at 600 nm (OD 600 ) of 0.4 prior to our experiments.Then each culture was divided into two aliquots, 20 ml each and cells were harvested by centrifugation at 6,0006g for 15 min.at 37uC.Furthermore the supernatant was removed and cells were resuspended in a BHI broth that was acidified with a 6 N HCL solution (Sigma) to pH 2.5.These tubes were incubated with shaking (180 rpm) at 37uC.At time point zero (t = 0) and after 10, 20 and 30 minutes intervals samples were taken from each culture, serially diluted with phosphate-buffered saline (16PBS) and plated on BHI agar plates.The colony forming units (CFU) were counted after overnight incubation at 37uC.The percentage of survivors was calculated by comparing the number of survivors in BHI (pH 2.5) after 10, 20 and 30 minutes with the number of cells in BHI (pH 2.5) at t = 0, respectively.Results are reported as means of data collected from three independent biological replicates.

Hydrogen peroxide sensitivity assay
BHI broth was used to dilute a 3% hydrogen peroxide (H 2 O 2 ) solution (Ottmar Fischer, Germany) to obtain 0.045% H 2 O 2 final concentration.
Overnight cultures in 10 ml BHI broth (in 100 ml Erlenmeyer flasks) were prepared and were diluted 1:50 in 20 ml fresh BHI broth or in 20 ml of different fresh BHI supplemented with 0.045% H 2 O 2 (in 100 ml Erlenmeyer flasks) as mentioned before.The optical density (OD 600 ) of the bacterial culture was measured at the indicated times after incubation at 37uC (180 rpm, Unitron, Infors).

Macrophage survival assay
P388D1 murine macrophages, cultured in 24-well plates in RPMI (Life Technologies) supplemented with 10% fetal calf serum (FCS) and 1% nonessential amino acids (NEAs), were infected with 1610 7 bacteria to obtain an MOI of 10.After 30 min.RPMI was removed, and cells were washed twice with 16PBS and incubated for 1 h in RPMI medium containing 50 mg/ml gentamicin.Macrophages were then washed three times with 16PBS and lysed with ice-cold 0.2% Triton X-100 in H 2 O.The released bacteria were plated on BHI agar plates in appropriate dilutions and quantified after overnight incubation at 37uC.
For the determination of macrophage survival rates the CFU numbers in the inoculum of the wild-type and the mutants used to infect P388D1 were compared and a factor was calculated.The wild-type factor was set to 1 and depending on the CFU numbers of the mutant inoculums their factor was ,1 or .1.For the calculation of the survival in % we set the CFU number of wildtype to 100% and percentage survival of the mutants was set in relation to wild-type.

cDNA synthesis and quantitative Real-Time Polymerase Chain Reaction
Messenger RNA levels of lmo0515, lmo1580 and lmo2673 isolated from extracellular grown bacteria in BHI and intracellular replicating bacteria in murine macrophages as previously described [24] were assessed in L. monocytogenes wild-type strain using quantitative Real-Time-PCR (qRT-PCR).Complementary DNA (cDNA) was obtained from bacteria from each setting by reverese transcription of RNA isolated and purified using the RNeasy Mini kit (Qiagen).Triplicates were performed for each forward and reverse primer pair combination.Primers were purchased from Qiagen (Quantitect Primer Assay) (see Table 2).
Primers were diluted to 1 pmol/ml for further procedure and qRT-PCR was run (7900 HT Fast Real Time System, Applied Biosystems).16sRNA was used as for normalization and calculation of relative expression.
Threshold cycle values (CT) of the tested genes were determined and normalized expression of each target gene was given as the DCT between the log2 transformed CT of the target gene and the log2 transformed CT of the internal control (ACTB).Log2 transformed gene expression levels (DCT) of each target gene for i5ntra-and extracellular bacteria were expressed as log2 differences from control ( = log2 DDCT method).Data was acquired and analyzed with the SDS 2.3 and RQ-Manager 1.2, respectively.

Animals and insects
Six to eight week-old female BALB/c mice, purchased from Harlan Winkelmann (Borchen, Germany), were kept at our breeding facilities in specific-pathogen-free conditions and used in all experiments.Galleria mellonella larvae, purchased from fauna topics (Marbach, Germany) were reared at 32uC in darkness and on an artificial diet (22% maize meal, 22% wheat germ, 11% dry yeast, 17.5% bee wax, 11% honey and 11% glycerine) prior to use.Last instar larvae, each weighing between 250 and 350 mg, were used in all experiments [20].

Insect and animal models of infection
In all experiments, fresh cultures of bacteria, prepared from an overnight culture, were used.Briefly, bacteria were grown in Brain heart Infusion (BHI) at 37uC, harvested in the exponential growth phase and washed twice with 16PBS.The pellet was resuspended in 16PBS and the bacterial concentration was calibrated by optical absorption.Further dilutions were prepared in 16PBS to obtain required numbers of bacteria for infection.The infection of G. mellonella was performed as previously described by Mukkerjee et al [20].Briefly, the human pathogenic L. monocytogenes and its isogenic usp deletion mutants or complemeted strains were separately injected (10 6 CFU/larva) into the hemocoel of the last instar larvae and the infection was monitored at 37uC.
In all experiments, fresh cultures of bacteria, prepared from an overnight culture, were used.Briefly, bacteria were grown in Brain heart Infusion (BHI) at 37uC, harvested in the exponential growth phase and washed twice with 16PBS.The pellet was resuspended in 16PBS and the bacterial concentration was calibrated by optical absorption.Further dilutions were prepared in 16PBS to obtain required numbers of bacteria for infection.
Primary mice infections in vivo infection with L. monocytogenes wild-type, Dlmo0515, Dlmo1580, Dlmo2673 or Dlmo0515Dl-mo1580Dlmo2673 were performed by an intravenous injection of viable bacteria in a volume of 0.2 ml of 16PBS.Spleens and livers were harvested three days after infection.Bacterial growth in spleens and livers was determined by plating 10-fold serial dilutions of the organ homogenates on BHI agar plates.Colonies were counted after 24 h of incubation at 37uC.

Ethics statement
This study was carried out in strict accordance with the regulation of the National Protection Animal Act (17-9a Tierschutzgesetz).The protocol was approved by the local Committee on the Ethics of Animal Experiments (Regierungsbezirk Mittelhessen) and permission was given by the local authority (Regierungspraesidium Giessen, Permit Number: GI 15/5-Nr.63/2007).

Statistical analysis
All experimental work was performed for a minimum of three times.Significant differences between two values were compared with a paired Student's t-test.Values were considered significantly different when P,0.05.

Comparative genomic analysis and promoter prediction
All three Usps of L. monocytogenes harbor an UspA domain based on the Pfam analysis (data not shown), and thus represent paralogues of the UspA family of E. coli [1].The structure and function of UspA is highly conserved among several bacteria and eukaryotic organisms.In line with this, comparative analysis in 19 listerial strains including all serotypes using GECO [25] (cut-off protein identity 80%, 90%) revealed a common chromosomal location for all three usp genes (Figure S1, S2, S3).Previously identified regulatory RNAs [26] were not located in the flanking regions of the usp genes.Furthermore, promoter box analysis of the upstream regions indicated that all usp genes habour a s B box upstream of their trancriptional start sites (Figure 1).
Universal stress proteins contribute to extracellular survival of L. monocytogenes at low pH condition L. monocytogenes is able to resist and survive at low pH conditions, which may occur in acidic food, in the gastric milieu and within the macrophage phagosome [27,28].In order to assess the role of listerial Usps at low pH environment we challenged exponentially grown L. monocytogenes and its isogenic usp deletion mutants (Dlmo0515, Dlmo1580 and Dlmo2673) with acidic BHI-medium (pH = 2.5) and determined the percentage of CFU at 10, 20 and 30 min after inoculation (Figure 2).
The CFU counts of both, Dlmo0515 and Dlmo1580 were significantly lower compared to wild-type bacteria at each time point.We observed no significant difference in survival rate between wild-type and Dlmo2673.Dlmo1580 was most susceptible to an increase in acid over the time course which was reflected by a more than 100 fold lower survival rate of the culture as compared to the wild-type strain.
Universal stress proteins are crucial for oxidative stress resistance of L. monocytogenes L. monocytogenes is known to be resistant to oxidative stress, occurring in the macrophage phagosome, where reactive oxygen species (ROS), such as H 2 O 2 are produced to kill engulfed pathogens [29].
To elucidate the role of listerial Usps in response to oxidative stress, we determined the survival of Dlmo0515, Dlmo1580 and Dlmo2673 using optical density measurement in BHI medium supplemented with H 2 O 2 following a 3 h incubation period (Figure 3).
Exposure to H 2 O 2 significantly impaired the growth of the mutants compared to the wild-type strain (OD 600 = 0.55).While growth attenuation was moderate in Dlmo0515 (OD 600 = 0.3), a dramatic effect was seen for the lmo2673 mutant (OD 600 = 0.16).The strongest effect was observed in Dlmo1580 with barely any growth detectable at any observation point.These results indicate a gradual order in susceptibility to oxidative stress in the three usp mutants as follows: Dlmo1580.Dlmo2673.Dlmo0515.

Survival attenuation of Dlmo1580 and Dlmo2673 in murine macrophages
Macrophages play a key role in the phagocytosis and intracellular killing of L. monocytogenes by generating ROS and acidic conditions in the phagosome.To examine the impact of Usps for intracellular survival, we determined the number of CFU isolated from P388D1 murine macrophages upon infection with either wild-type strain or the usp deletion mutants [30].
In agreement with our results at low pH condition and the oxidative stress response, the Dlmo1580 deletion had the strongest effect on intracellular listerial survival.Compared to the wild-type strain, only 72% of Dlmo1580 were able to resist the hostile macrophage environment.Dlmo2673 exhibited similar survival attenuation with 76% viable bacteria compared to wild-type strain.No significant effect on intracellular survival was detected in the Dlmo0515 mutant (Figure 4).To confirm that the usp genes are intracellular induced we appllied quantitative RT-PCR.The analysis showed that usp transcripts obtained from intracellular versus extracellular grown bacteria displayed a strong induction of usp genes (Figure 5).

Decreased virulence of usp mutants in an invertebrate in vivo model
G. mellonella is currently a well established model host for studying the virulence attributes of the human pathogenic L. monocytogenes [20].In this work, we used this invertebrate model to evaluate the pathogenicity of listerial usp deletion mutants.G. mellonella larvae were infected with pathogenic EGD-e and its isogenic usp mutants namely Dlmo1580, Dlmo2673 and Dlmo0515 respectively.Survival rate of the infected larvae was determined at 37uC for over a period of 7 days.
Attenuated virulence associated with higher survival rates of larvae (Figure 6) were observed in Dlmo1580, Dlmo2673 and Dlmo0515 (75%, 65% and 50% respectively) as compared to wildtype infection (37%).The difference between Dlmo0515 and wildtype did however not reach statistical significance.As observed previously with intracellular growth in macrophages, the Dlmo1580 mutation had the strongest effect on the pathogenicity, exhibiting nearly avirulent characteristics.A similar result was obtained for the triple mutant Dlmo0515Dlmo1580Dlmo2673 (Figure S4).To confirm that the deletion of usp genes is responsible for the effects in invertebrate infections we complemented the single usp mutants.Complementation restored the ability to kill insect larvae to levels comparable to the wild-type bacteria (Figure S5).
We observed no differences in growth of usp mutants and wildtype strain when cultured in BHI (data not shown) which excludes the possibility that varying numbers of inoculum CFUs of usp deletion mutants affected the survival of insect larvae.

Growth and survival of Listeria usp deletion mutants is profoundly impaired in a murine infection model
Although several studies have investigated the role of Usps in different bacteria, an important role of Usps in vivo has only been shown for the regulation of growth in S. typhimurium [12] and M. tuberculosis [6].To elucidate the influence of listerial Usps on the growth and survival in vivo, we injected BALB/c mice with three different usp deletion mutants or wild-type and determined the number of bacteria isolated from the liver.
Remarkably, the number of CFU of all mutants compared to wild-type was dramatically diminished in this mouse model (Figure 7).The number of viable bacteria lacking the respective usp gene was significantly lower in livers and spleens of infected mice.In this setting, growth of Dlmo0515 was reduced to 22% in spleen and 32% in liver as compared to wild-type strain.The number of Dlmo2673 was reduced to 68% and 60%, and the Dlmo1580 count declined to 38% and 68%, respectively.The isogenic usp triple mutant showed comparable results as the single usp mutants.

Discussion
L. monocytogenes is capable of withstanding a variety of stressors encountered in nature and within infected host cells [18].The ability to rapidly adjust to the changing environment is essential for its pathogenic lifestyle [18].In this study, we provide strong evidence that universal stress proteins (Usps) are highly conserved among Listeriae and are important for resistance and growth of L. monocytogenes by investigating usp deletion mutants exposed to different stress conditions both in vitro and in vivo.
Universal stress proteins were shown to be important in response to stress in gram-negative bacteria, including E. coli [31], S. typhimurium [12] and Azospirillium brasilense [32].However, the role of these proteins in gram-positive bacteria is not as well delineated.We have previously revealed an induction of three genes, lmo0515, lmo2673 and lmo1580 in L. monocytogenes in response to heat shock and acid stress [33,34].In accordance with effects described for E. coli lacking the uspA gene, L. monocytogenes usp deletion mutations investigated in this study displayed impaired capability to resist exposure to H 2 O 2 and low pH conditions.Variable susceptibility to stress conditions between deletion mutants may represent a nonredundant role of Usps in stress responses.Similar observations were made for E. coli and addressed in a recent study testing several usp deletion mutants [31].Nachin et al. uncovered that the UspA proteins differ in their responses to oxidative stress and DNA damaging agents and defined similarities as well as differences in expression pattern based on their biological role in stress response [31].Thus, certain environmental changes may not require the induction of all usp genes, but allow adjustment depending on the specialized role of a particular Usp.
Generally, usp genes are expressed in monocistronic units and the production of UspA seems to be primarily regulated at the level of transcription initiation [1].We and others have previously reported an important role for s B , a transcription factor that controls an inducible regulon in L. monocytogenes induced following challenge to different stress conditions, such as acids, high osmolarity or carbohydrate starvation [17,34,35].As previously reported, a single s B promoter has been identified for lmo1580; and promoter analysis revealed s B boxes for lmo2673 and lmo0515.
Thus, it appears likely that s B possesses a major role in stress induced transcriptional regulation of usp genes in L. monocytogenes.Furthermore, phenolic acids and fructose-6-phosphate were shown to influence usp gene expression in Lactobacillus plantarum and E coli, respectively.L. monocytogenes mobilizes a large set of metabolic genes in response to stress [24].Especially, genes of the carbohydrate metabolism are strongly expressed.Possible effects of metabolic constituents on usp gene regulation may therefore not be excluded and require further investigation.[15,36].
In this regard, the temporal and coordinated activities of different Usps of L. monocytogenes may enable the bacteria to survive in acid-rich niches such as in the upper intestinal tract or mediate resistance to oxidative stress and bile acids in the spleen and liver.
Following ingestion by phagocytic cells, Listeriae are faced with the hostile environment in the phagosome [18].As shown previously, virulence and intracellular growth adaption of L. monocytogenes depends on a concerted gene expression program including the upregulation of universal stress proteins [24,37].In this study, we demonstrate that survival of Listeriae in murine macrophages is dependent on the presence of Usps.This was reflected by decreased virulence, resulting in a significant reduction of bacteria lacking the respective usp genes.In addition we observed intracellular transcriptional induction of all three usp genes which supports the Figure 5. qRT-PCR analysis of lmo0515, lmo1580 and lmo2673 expressed in L. monocytogenes EGD-e.The graph displays the relative expression of the respective usp gene in the intracellular vs. extracellular setting.High fold changes indicate that the particular gene was higher expressed in intracellularly localized bacteria as compared to expression in bacteria that were grown in BHI.The expression of lmo0515 and lmo2673 is strongly enhanced in intracellular pathogens, while levels of lmo1580 remain relatively constant.This is consistent with the observation made in this study, which shows a strong dependence on lmo2673 and lmo0515 in the murine infection model experiments as compared to extracellular in vitro challenges, while lmo1580 seems to play an important role in both conditions.doi:10.1371/journal.pone.0024965.g005evidence that the particular stress proteins are involved in the intracellular pathogenic lifestyle of L. monocytogenes.
The impact of Usps in infection has only been examined for a few bacteria and their role, if any, in gram-positive pathogens has not been described [6,12,31,37].For this reason, we further characterized the effect of listerial Usps in in vivo infection models.First we tested their effects on the viability of G. mellonella larvae, a recently described alternative infection model for L. monocytogenes Survival of mutant bacteria are impaired in both organs, reflecting a role of Usps in listerial resistance and growth in vivo.Statistically significant differences were identified using a two-tailed Student t test.(*, p,0.05).doi:10.1371/journal.pone.0024965.g007[20].Lack of usp genes resulted in improved survival of infected larvae as compared to wild-type infection.In accordance with our in vitro results, Dlmo1580 was nearly avirulent demonstrating its importance in pathogenicity of L. monocytogenes.As also demonstrated here, Usps contribute strongly to the survival and proliferation of these bacteria in livers and spleens of infected mice, while no major differences were apparent when comparing both organs.We have also constructed a triple mutant to investigate the cooperative nature of the three usp genes.We could not observe any synergistic effect for the usp triple mutant in mice experiments, but in the invertebrate infection model the isogenic triple mutant indicated a similar survival rate for larvae infected with Dlmo1580.Thus, we suspect that Dlmo1580 might be the major player among the three usp genes involved in pathogenicity.
Previously, Usps were implicated in the resistance to antibiotics [38], biofilm formation by P. gingivalis [10] and survival of P. aeruginosa under anaerobic conditions [7].In this study however, we did not identify a significant impact of usp gene deletion on biofilm formation or altered resistance under anaerobic/microaerophilic conditions and in response to treatment with several antibiotics (data not shown).
In conclusion, we have identified an important role of three universal stress proteins in resistance and survival of L. monocytogenes in response to low pH conditions and oxidative stress as well as intracellular survival within macrophages.We subsequently confirmed our observations in two in vivo infection models and show for the first time that universal stress proteins are important for survival and growth in the vertebrate and invertebrate hosts.Finally, we propose the designation of genes for lmo0515, lmo1580 and lmo2673 as uspL-1 (universal stress protein Listeria), uspL-2 and uspL-3, respectively in the species L. monocytogenes.

Figure 1 .
Figure 1.Transcriptional overview of chromosomal loci of lmo0515 (A), lmo1580 (B), and lmo2673 (C) derived from sequencing data of L. monocytogenes (data not shown).s B boxes are depicted as orange arrows, the primary transcripts from their start sites by grey bars, the ribosome binding site as a yellow box and the respective coding sequence from their start sites by green bars.doi:10.1371/journal.pone.0024965.g001

Figure 2 .Figure 3 .
Figure 2. Effect of low acidic condition (pH = 2.5) on survival of usp deletion mutants compared to L. monocytogenes EGD-e wildtype.Dlmo0515 and Dlmo1580 display decreased growth at all observations, while impaired resistance in Dlmo2673 was visible only at 20 min post challenge.Statistically significant differences were identified using a two-tailed Student t test.(*, p,0.05 and **, p,0.005).doi:10.1371/journal.pone.0024965.g002

Figure 4 .
Figure 4.The intracellular survival of L. monocytogenes EGD-e wild-type and usp deletion mutants was assessed in murine macrophages according to [30].Intracellular bacteria burden was decreased in macrophages inoculated with Dlmo1580 and Dlmo2673, but no effect was visible for Dlmo0515.Statistically significant differences were identified using a two-tailed Student t test.(*, p,0.05 and **, p,0.005).doi:10.1371/journal.pone.0024965.g004

Figure 6 .Figure 7 .
Figure6.Survival of G. mellonella larvae after inoculation with usp deletion mutants.As compared to L. monocytogenes EGD-e wild-type, all usp deletion mutants exhibited impaired virulence, resulting in higher survival rates of G. mellonella larvae.Strongest effects were observed for Dlmo1580 and Dlmo2673, while deletion of lmo0515 was not associated with significant increase in survival of G. mellonella.Results represent mean values of at least three independent experiments with a total of 80 larvae per treatment.Statistically significant differences were identified using a two-tailed Student t test.(***, p,0.0005 and ns-not significant).doi:10.1371/journal.pone.0024965.g006

Figure S1
Figure S1 Comparative analysis of flanking regions of listerial universal stress protein lmo0515 among 19 L. monocytogenes species including all known serotypes.(PDF)

Table 2 .
Primers used in this study.