MexEF-OprN Efflux Pump Exports the Pseudomonas Quinolone Signal (PQS) Precursor HHQ (4-hydroxy-2-heptylquinoline)

Bacterial cells have evolved the capacity to communicate between each other via small diffusible chemical signals termed autoinducers. Pseudomonas aeruginosa is an opportunistic pathogen involved, among others, in cystic fibrosis complications. Virulence of P. aeruginosa relies on its ability to produce a number of autoinducers, including 4-hydroxy-2-alkylquinolines (HAQ). In a cell density-dependent manner, accumulated signals induce the expression of multiple targets, especially virulence factors. This phenomenon, called quorum sensing, promotes bacterial capacity to cause disease. Furthermore, P. aeruginosa possesses many multidrug efflux pumps conferring adaptive resistance to antibiotics. Activity of some of these efflux pumps also influences quorum sensing. The present study demonstrates that the MexEF-OprN efflux pump modulates quorum sensing through secretion of a signalling molecule belonging to the HAQ family. Moreover, activation of MexEF-OprN reduces virulence factor expression and swarming motility. Since MexEF-OprN can be activated in infected hosts even in the absence of antibiotic selective pressure, it could promote establishment of chronic infections in the lungs of people suffering from cystic fibrosis, thus diminishing the immune response to virulence factors. Therapeutic drugs that affect multidrug efflux pumps and HAQ-mediated quorum sensing would be valuable tools to shut down bacterial virulence.


Introduction
Chronic infections caused by the opportunistic pathogen Pseudomonas aeruginosa are strongly associated with cystic fibrosis (CF)-related complications such as lung damage and airway obstruction [1]. These complications result from the exacerbated inflammation associated with the chronic infection of cystic fibrotic lungs [2]. Antibiotic therapies aiming at long-term remission are usually inefficient and, consequently, lungs of most individuals suffering from CF are permanently colonized by P. aeruginosa [3]. P. aeruginosa is resistant to a wide diversity of important antimicrobial agents [4], which is largely due to the activity of multiple multidrug efflux pumps. Among the various types of efflux pumps found in Gram-negative bacteria, the RND (Resistance-Nodulation-cell Division) family plays an important role in the adaptive resistance to antibiotics [5,6]. In P. aeruginosa, activation of the MexEF-OprN RND-type efflux pump gives rise to, among others, chloramphenicol, fluoroquinolones, trimethoprim and triclosan resistance [7]. This efflux pump is encoded by the mexEF-oprN operon and is positively controlled by MexT, a transcriptional regulator belonging to the LysR family [8,9]. The MexS protein also influences the MexEF-oprN efflux state of activity by a mechanism yet to be determined. MexS presents homologies with Zn 2+ -dependent oxidoreductases/deshydrogenases, some of which associated with amino acid metabolism.
Mutations in the mexS gene lead to a dramatic increase in mexEF-oprN transcription [10,11]. Consequently, some authors have suggested that mexS, together with mexEF-oprN, could be involved in detoxification [12]. In addition to its role in resistance to antibiotics, the MexEF-OprN efflux pump affects many quorum sensing (QS)-dependent virulence phenotypes (described below).
P. aeruginosa QS depends on the transcriptional regulators LasR, RhlR and MvfR (PqsR) as well as on their cognate autoinducer synthases LasI, RhlI and PqsA-D/PqsH, respectively [13]. LasI and RhlI synthesize the autoinducers 3-oxo-dodecanoyl-homoserine lactone (3-oxo-C 12 -HSL) and butanoyl-homoserine lactone (C 4 -HSL) respectively, whereas PqsH catalyzes the final step in the synthesis of 3,4-dihydroxy-2-heptylquinoline [14,15,16], known as the Pseudomonas Quinolone Signal (PQS) [17]. As the bacterial cell population grows, autoinducers are synthesized, freely diffuse out or are actively exported across Gram negative bacterial membranes and, eventually, exceed a threshold concentration at which point quorum is reached [18,19]. An autoinducer bind to, and activate, its cognate response regulator which then initiate or repress the transcription of target genes. Activated LasR and RhlR QS regulators exert positive feedback on their own QS system but also they can influence activation of other systems. For instance, LasR stimulates the transcription of the RhlRI and MvfR QS systems, and directly controls the expression of pqsH [15,20,21]. In this regard, the MvfR system is unique compared to other known QS systems since the expression of its main autoinducer PQS is not directly controlled by its cognate regulator. Instead, PQS concentration relies on 4-hydroxy-2-heptylquinoline (HHQ) production; the product of pqsABCDE operon and the direct precursor of PQS [14]. The pqsABCDE operon is regulated by MvfR and is involved in the biosynthesis of many other 4-hydroxy-2-alkylquinolines (HAQs) [14,22].
Thus, to elucidate the molecular phenomena driving QS modulation under MexEF-OprN activity, we used a mexS 2 mutant and various spontaneous nfxC-type mutants of P. aeruginosa strain PA14. These mutants can overexpress mexEF-oprN more than 300fold compared to the wild-type strain. This strong efflux activity allowed us to demonstrate that an RND-type efflux pump exports the HAQ QS signalling molecule HHQ, the precursor of the PQS, and that this leads to the QS defects characterizing nfxC-type mutants.

Results
A P. aeruginosa mexS 2 mutant overexpressing MexEF-OprN fails to convert most HHQ into PQS To further characterize the effects of MexEF-OprN efflux pump activation on QS, the concentrations of the best known MvfRdependent QS signaling molecules were compared between the wild-type P. aeruginosa strain PA14 and its isogenic mexS 2 mutant (MGL01), which represents an nfxC phenotype. Loss of mexS results in uncontrolled overexpression of the MexEF-OprN pump (see Experimental procedures) [10,11]. In MGL01 cultures, the concentration of HHQ, the direct precursor of PQS, is about four-fold higher than in PA14 cultures at an OD 600 = 5 ( Fig. 1). However, and unexpectedly, PQS concentration, at the same growth stage, is about two-fold lower in cultures of MGL01 compared to that of the wild-type strain (Fig. 1). Similarly to HHQ and PQS, the synthesis of the other main HAQ, HQNO, depends on PqsABCD [14,22]. However, in contrast with PQS, HQNO synthesis is not dependent on the presence of HHQ [14]. Accordingly, there is no significant difference between HQNO concentrations quantified in the wild-type and MGL01 cultures (Fig. 1).
To verify whether perturbed HHQ and PQS production in MGL01 is due to the mexS mutation per se, a complemented mexS 2 mutant strain MGL01::pML01 was used. As shown in figure S1, HAQ phenotypes are restored to those of the wild-type in the complemented strain. Noteworthy, many P. aeruginosa strains carry an 8 bp insertion within the mexT gene that causes a frame shift and silences mexEF-oprN [10]. Blast analysis against PAO1 mexT reveals that strain PA14 possesses a functional mexT gene due to the deletion of this insertion, restoring the reading frame. This is in agreement with observations made by Maseda et al. (2000) using various PAO1 strains [10]. Nevertheless, in PA14, the MexEF-OprN efflux pump is only expressed at a basal level under experimental conditions (data not shown). Similarly, a mexE 2 mutant (MGL04) displays no defect in HAQ production when compared to wild-type PA14, testifying that basal levels of HAQ efflux via MexEF-OprN are not sufficient to affect HAQ phenotypes in the wild-type background (Fig. S1). To determine if HAQ modulation is specific to overexpression of the MexEF- OprN RND-type efflux pump, we verified the production of HAQs in four other constitutively expressing RND efflux pump mutants, i.e. in nfxB 2 , mexZ 2 , nalC 2 and mexL 2 mutants. These mutants overproduce MexCD-OprJ, MexXY-OprM, MexAB-OprM and MexJK-OprM, respectively. We found that none of these other efflux pumps influences HHQ and PQS production (Fig. S2). Together, these results indicate that HHQ and PQS are specifically modulated by MexEF-OprN in the MGL01 mexS 2 mutant.
Expression of the pqsH gene is upregulated in a PA14 mexS 2 mutant To understand the reason for the HHQ and PQS imbalance characterizing the mexS 2 mutant, the expression patterns of appropriate QS regulators and their related genes were studied using transcriptional and translational reporter fusions. We found that the transcriptional activity of the mvfR promoter is nearly two-times higher in MGL01 than in the wild-type strain ( Fig. 2A). As expected, the transcription of the pqsABCDE operon, which is activated by MvfR, was found to be upregulated as well ( Fig. 2A). The molecular basis explaining this increased expression is unknown (discussed below). Since mvfR and pqsH are positively controlled by the LasRI QS system, it was interesting to investigate the expression of the lasR and lasI genes in MGL01. The lasI translational reporter fusion revealed that the 3oxo-C 12 -HSL synthase is half-fold more expressed in MGL01 than in PA14 ( Fig. 2A). Accordingly, 3-oxo-C 12 -HSL concentrations quantified by LC-MS/MS are higher in MGL01 cultures when compared to PA14 (Fig. 2B). In contrast, C 4 -HSL concentrations are lower in MGL01 cultures (Fig. 2B).
Intriguingly, although PQS, the product of PqsH activity, is produced in lower concentrations in MGL01, mvfR and pqsABCDE are up-regulated in this strain. Thus, it was interesting to verify whether the decrease in PQS is due to a decreased transcription of pqsH in MGL01 strain. This was verified using a pqsH promoter-lacZ transcriptional fusion chromosomally integrated into the wild-type and MGL01 strains, yielding PA14 (pML03) and MGL01 (pML03) strains, respectively. Unexpectedly, pqsH expression is upregulated in MGL01 compared to the wild-type ( Fig. 2A). Yet, this was not reflected in a higher production of PQS, although mexS 2 mutant produces high concentrations of the PQS precursor, HHQ ( Fig. 1).
Trans-expression of pqsH and HHQ supplementation do not restore PQS production in mexS 2 mutants In order to verify whether the reduced PQS production in the mexS 2 mutant is caused by a posttranscriptional mechanism, we introduced a medium copy number plasmid carrying pqsH under a constitutive promoter (pML04) into the wild-type and MGL01 strains, giving strains PA14 (pML04) and MGL01 (pML04) respectively. Also, to promote HHQ availability to PqsH, exogenous HHQ was added to the cultures. As expected, PA14 (pML04) rapidly consumes exogenous HHQ to produce PQS while the PA14 (pUCP26) control strain presents a more gradual HHQ consumption with a corresponding simultaneous PQS production (Fig. 3). In contrast, we observed a slight decrease in available HHQ in MGL01 (pML04), with a concomitant limited conversion into PQS (Fig. 3). Thus PQS production is only partially restored in a mexS 2 background by pqsH gene upregulation and exogenous supplementation of HHQ.

Constitutively expressing MexEF-OprN mutant cells contain less HHQ than wild-type cells
The above results demonstrate that HAQ concentrations in MGL01 are not only directly influenced by the transcriptional and translational status of lasRI, mvfR, pqsABCDE and pqsH genes but also by another unidentified mechanism. We thus hypothesized that the activity of the MexEF-OprN efflux pump directly modulates HAQ intracellular concentrations, the fact that could explain the weak PQS synthesis by the mexS 2 mutant cells. This was first verified by directly quantifying cell-associated HHQ and PQS molecules in PA14 and MGL01. As shown in figure 4A, proportions of cell-associated HHQ are lower in MGL01 than in the wild-type strain PA14. This result is even more striking when considering that MGL01 is a hyper HHQ producer (Fig. 1). Nonetheless, a higher concentration of cell-associated PQS is observed in the wild-type strain PA14 ( Figure 4A), which is consistent with the higher production of PQS in PA14 cultures ( Fig. 1).
In light of these results, we compared the intracellular availability of PQS and HHQ for MvfR binding under MexEF-OprN overexpression. PQS or HHQ was exogenously added to cultures of an HAQ-deficient PA14 strain (pqsA 2 pqsH 2 double mutant), or of its isogenic nfxC mutant (MGL02), carrying the pqsA-lacZ transcriptional reporter plasmid pGX5 [31,32]. We found that addition of PQS results in a stronger induction from the pqsA promoter in MGL02 (pGX5) than in PA14 pqsA 2 pqsH 2 (pGX5) (Fig. 4B). This observation is likely to result from the strong activating effect of PQS on the MvfR transcriptional regulator in this PA14 nfxC mutant [14,32]. Indeed, as described previously, the MGL01 mexS 2 mutant is characterized by a stronger induction from the pqsA promoter than the wild-type strain (Fig. 2). Strickingly, MGL02 (pGX5) presents a lower HHQdependent induction of the lacZ reporter gene, when compared to that of the PA14 pqsA 2 pqsH 2 double mutant (Fig. 4B). These results indicate that HHQ is less efficient in activating MvfR when MexEF-OprN is constitutively overexpressed, suggesting that HHQ is rapidly pumped out of the cells, reducing its availability for MvfR activation.

The MexEF-OprN efflux pump exports HHQ
To further verify this hypothesis, we quantified HAQ concentrations while blocking the efflux activity of MexEF-OprN. This was accomplished by assaying HAQs in cultures exposed to an efflux pump inhibitor (EPI): the MC-207,110 molecule [33]. Because of its growth-inhibitory effect, MC-207,110 was used at low concentrations (see experimental procedures). HAQs from supernatant as well as from washed cells were quantified by LC-MS/MS. Remarkably, cell-associated HHQ increased in EPItreated MGL01 cultures (Fig. 5A), whereas the corresponding culture supernatants showed lower HHQ concentrations when compared to untreated cultures (Fig. 5B). Moreover, PQS concentrations are considerably higher in both of the washed cell samples and their corresponding supernatants in EPI-treated MGL01 cultures when compared to controls ( Figure 5C-D). Taken together, these results support our hypothesis that, in mexS 2 mutants, where the MexEF-OprN efflux pump is constitutively overexpressed, much less intracellular HHQ is available to the PqsH enzyme for PQS synthesis.
Finally, to conclusively confirm the importance of the MexEF-OprN efflux activity in the modulation of HAQ production, we constructed a mexS 2 mexE 2 double mutant of strain PA14, giving strain MGL03. As expected, figure 6 is showing that PQS production is increased to wild-type levels by the inactivation of mexE while HHQ concentrations are decreased, although not fully restored to that of the wild-type, in MGL03 (discussed below). Moreover, the mexE knockout restores QS-related virulence phenotypes in MGL01 such as swarming motility, pyocyanin production and biofilm formation (Fig. 6). As expected mexS 2 complementation in MGL01 with pML01 also restores the phenotypes to that of the wild-type (Fig. S3). Taken together, these results confirm HHQ as a substrate of the MexEF-OprN efflux system, which affects its intracellular availability for PqsHmediated conversion into PQS and consequently, modulate QSdependent virulence traits. Discussion P. aeruginosa is highly resistant to a large spectrum of antimicrobial agents as it possesses, among other factors, an array of both constitutively active and inducible efflux pumps. In addition to their well-studied role as major antibiotic resistance determinants, these pumps are currently thought to play other unexpected functions, including QS modulation [29,30,34,35,36]. The role of the MexEF-OprN efflux pump in the P. aeruginosa biology is closely related to the mexS gene, whose function is still misunderstood. K. Poole (2005) has proposed that MexS could serve as a detoxifying enzyme for a yet unidentified metabolite [12]. Additionally, the MexEF-OprN efflux pump could be important for this metabolite secretion in non functional mexS cells [12]. However, the natural substrate of MexEF-OprN is unknown. Based on our results and hypothesis put forward by K. Poole, it is tempting to speculate that such a molecule could be structurally related to quinolones. Indeed, the present study shows that the MexEF-OprN efflux pump exports the PQS precursor, HHQ.
Results presented in figure 1 illustrate well the link that exists between HHQ synthesis and PQS accumulation. In the wild-type strain PA14, PQS accumulates while the availability of HHQ remains stable and low, indicating that the synthesis rate of HHQ is similar to that of PQS, as we have previously reported [14]. This equilibrium is disturbed in the mexS 2 mutant, MGL01, since HHQ accumulates while PQS is poorly synthesized (Fig. 1). We observed that the LasR-controlled QS genes, including mvfR, are upregulated in MGL01 ( Fig. 2A). However, mvfR upregulation alone cannot explain why the pqsABCDE operon, involved in HHQ synthesis, is upregulated in the weak PQS producer strain MGL01. This is intriguing, especially when considering that PQS is the primary activating ligand of MvfR. Unknown factors, independent of PQS or HHQ, in mexS 2 mutants might be exerting a positive effect on the transcription of pqsABCDE. This hypothesis is currently under investigation in our laboratory. Still, it is plausible that upregulation of LasR-controlled QS genes (lasR regulon) might result from the increased 3-oxo-C 12 -HSL concentration observed in MGL01 cultures, as shown in figure 2B [30,37]. Interestingly, MexT, which is highly expressed in nfxCtype mutants, blocks the RhlR/C 4 -HSL-dependent activation of the mexAB-OprM operon, an efflux pump involved in 3-oxo-C 12 -HSL export [29,30,37]. Therefore, MexT-dependent downregulation of mexAB-OprM could well be responsible for the 3-oxo-C 12 -HSL accumulation seen with MGL01 cells, thus stimulating, in a positive feedback loop fashion, the LasR-controlled genes. Being a part of the LasR regulon, pqsH expression is upregulated in the MGL01 mexS 2 mutant, yet, the overproduced HHQ is poorly converted into PQS. Even higher expression of the pqsH gene from a constitutive plac promoter fails to complement the MGL01 PQS production defect (Fig. 3). Together, these results strongly suggest that most HHQ produced by MGL01 should be rapidly secreted out of cells and kept in the extracellular milieu ( Figs. 1 and 3). Accordingly, in a nfxC background, exogenously added HHQ fails to activate MvfR and consequently, to induce the pqsA-lacZ reporter construct (Fig. 4b).
At this stage of the study, results and clues we had pinpointed toward a role for the MexEF-OprN efflux pump in the export of HHQ. We thus designed experiments to confirm this hypothesis. We first showed that mexS 2 mutant cells, which produce much more HHQ than the wild-type, contain less cell-associated HHQ than the wild-type cells. HAQs are molecules that are thought to diffuse across, and get trapped into, lipid membranes. For instance most PQS is cell-bound [38]. PQS was also shown to bind lipid A molecules, while HHQ does not [39]. Thus, in MGL01, putative lipid A modifications are unlikely be responsible for the observed low cell-bound HHQ concentration. Instead, it is very probable that MexEF-OprN constitutive activity, which characterizes mexS 2 mutant cells, causes a rapid export of HHQ away from the intracellular PqsH enzyme, explaining why PQS is poorly synthesized in MGL01. Accordingly, the efflux pump inhibitor (EPI) MC-207,110 increases HHQ accumulation in MGL01 cells, seemingly just enough to induce PQS synthesis which must be concomitant with HHQ consumption (Fig. 5A) [33]. Finally, we show that knocking out mexE gene in the MGL01 background (MGL03 strain) restores PQS production to that of the wild-type levels, as well as some QS-dependent virulence phenotypes such as swarming motility, pyocyanin production and biofilm formation ( Fig. 6B) [11,24,26,30,40]. Interestingly, although PQS synthesis is restored in MGL03 strain, there is an upregulation in the expression of pqsABCDE demonstrated by the accumulation of HHQ when compared to the wild-type (Fig. 6A). This observation strengthens our hypothesis that, in mexS 2 mutants, the pqsABCDE operon may be subject to genetic control other than that exerted by the MvfR-PQS complex. Furthermore, results obtained with strain MGL03 are in accordance with those of Favre-Bonté et al. (2003), who showed that a mexE knock-out restores the biofilm as well as C 4 -HSL deficiencies characterizing the nfxC strain PAO1-BI relative to the wild-type phenotypes [23]. Similarly, Cosson et al. (2002) reported that such double mutant is as virulent as the wild-type P. aeruginosa in infection models [41]. Together, our results validate the role of the MexEF-OprN apparatus per se in the export of HHQ. Our finding finally validates the hypothesis put forward by Köhler et al. in 2001 that this efflux pump exports a precursor of PQS [24].
The MexEF-OprN is apparently inactive in most P. aeruginosa strains, permitting expression of QS-dependent virulence factors [10]. Nevertheless, isolates from chronic CF infections were shown to carry multiple mutations that simultaneously silence expression of QS-related virulence factors (e.g. lasR 2 and pqsABCDE 2 mutants) and enhance resistance to antibiotics [27]. Likewise, some studies noted the increased expression of mexEF- oprN in clinical isolates of P. aeruginosa, suggesting that a positive pressure occurs in vivo for nfxC clones selection [25,42,43,44]. Moreover, HHQ was found to be more abundant than PQS in a mammalian infection model, which is reminiscent of MGL01 mexS 2 mutant HAQs phenotypes [32,45]. Since QS is essential for P. aeruginosa virulence in various infection models, we believe that the expression status of the MexEF-OprN efflux pump could be an important determinant permitting the switch from an acute to a chronic infection ''mode''. Obviously, infected hosts can be submited to a strong antibiotherapy that selects for multiresistant P. aeruginosa. Interestingly however, mexEF-oprN constitutive expression has also been reported in the absence of selective pressure. Transcriptomic data from P. aeruginosa interacting with primary normal human airways epithelial cells revealed overexpression of the mexEF-oprN operon [46]. Also, frequency of nfxC emergence was seen to be 10-fold higher in vivo (model of acute pneumonia in rat), without antibiotics pressure, than in conventional culture media [43]. In light of these studies, we believe that prolonged P. aeruginosa exposure to some in vivo conditions alone might suffice to induce mexEF-oprN expression or select for nfxC mutants. Likewise, Fetar et al. (2010) recently showed that mexEF-OprN transcription is induced by in vitro nitrosative stress, a condition also known to occur during pulmonary infections [47]. Thus, in addition to some unknown environmental cues, selective pressures from in vivo environments might promote mexEF-oprN expression and chromosomal stability.
In conclusion, we propose that the MexEF-OprN efflux pump constitutes an important machinery that modulates the virulence of P. aeruginosa through the export of specific QS regulatory molecules, especially HHQ.

Experimental procedures
Bacterial strains, plasmids and media. Strains and plasmids used in this study are listed in Table 1. Bacteria were grown in Tryptic Soy Broth (TSB) (Difco) at 37uC. nfxC mutants were selected on TSB agar plates containing 500 mg/ml chloramphenicol. HHQ was synthesized as described [38] and was added to cultures at a concentration of 41 mM, when indicated.

Construction of plasmids and mutant strains
Construction of the mexS 2 mutant. The mexS 2 mutant used in this study was obtained from random mutagenesis using the pIT2 plasmid (ISlacZ/hah transposon) [48] during a screening for swarming deficient mutants (J. Tremblay and E. Déziel, unpublished data). This mutant is designated mexS::Tn5. The tetracycline resistance marker was excised from the insertion site using the Cre recombinase harbored by the pCRE2 plasmid; leaving a 63 amino acids insertion at leucine 185 [49]. This mutant strain is designated MGL01. To confirmed constitutive expression of the mexEF-oprN operon in MGL01, posttranscriptional status of mexE mRNA was assessed by qRT-PCR. Compared to the wild-type strain PA14, transcription of the mexE gene is increased 21-and 12-fold at OD 600 of 1.4 and 4.0, respectively. The fold change was calculated using the 2 2DDCt method [50]. The nadB gene was used as a housekeeping control [51]. To complement the mexS 2 mutation, the mexS gene was amplified using the inMexT-F (59-CGACGTCGTCGGAAAG-GCCGATG-39) and PA14_32440-R (59-CTAGCCAGGTT-GCACGATCATCCAAGAC-39) primers and cloned into the pGEM-T easy vector (Promega, Madison, WI, USA). The NotI fragment of the pGEM-T easy::mexS construct was then cloned into the NotI restriction site of the mini-CTX1 plasmid [52], giving pML01. This construct was then introduced into competent E. coli SM10lpir for mobilization into P. aeruginosa MGL01 by conjugation, to give MGL01 (pML01).
Construction of chromosomal pqsH-lacZ transcriptional reporter. The pqsH promoter region was amplified using the ppqsH-F (59-GGTACCTAAGGGGTTGACAGGAGC-39) and ppqs-H-R (59-GGATCCCCGTTGCTCCTTAGCAGC-39) primers, which contain the restriction sites KpnI and BamHI, respectively (underlined). The resulting 512 pb fragment was cloned into the corresponding sites of the mini-CTX-lacZ plasmid [55], giving pML03, which was then introduced into competent E. coli SM10lpir for mobilization into P. aeruginosa PA14 and MGL01 by conjugation, to give PA14 (pML03) and MGL01 (pML03), respectively. Clones carrying chromosomal insertion of the mini-CTX constructions were selected on tetracycline TSB plates. The tetracycline resistance cassette was excised from the chromosome using the flipase expressed from plasmid pFLP2 [53].
b-galactosidase assay. b-galactosidase activity was measured as described previously by Miller with slight modifications [57]. Briefly, cells were grown in TSB to various cell densities and then Detection and measurements of HAQs by LC/MS. Except when specified, 300 mL culture samples were taken at regular intervals, used for determination of growth (OD 600 ), and mixed with 300 ml methanol containing 75 mM of tetradeutero-PQS and 40 mM of tetradeutero-HHQ for final concentrations of 37.5 and 20 mM respectively, as internal standards [38]. After centrifugation, samples were directly injected for LC separation on an Agilent HP1100 HPLC system equipped with a 36150 mm C8 Luna reverse-phase column (Phenomenex). A 1% acidified water/acetonitrile gradient was used as the mobile phase at a flow rate of 0.4 ml?min 21 , split to 10% with a Valco Tee. A Quattro II (Waters) triple-quadrupole MS was used for molecule detection. Data acquisition was performed in positive ion mode with a scanning range of 100-400 Da. Precise quantification of HAQs was performed by MS/MS, as described previously [22,58].
Efflux pump inhibitor assays. The MC-207,110 molecule is an efflux pump inhibitor (EPI) and was kindly provided by Olga Lomovskaya from Mpex Pharmaceuticals [33]. The MexEF-OprN efflux pump is absolutely essential for the high chloramphenicol resistance feature of nfxC mutants, which are readily isolated on chloramphenicol plates. We thus evaluated the efficiency of MC-207,110 to decrease the activity of MexEF-OprN by its effect on the emergence of nfxC colonies. This was conducted by plating 50 mL of overnight wild-type cultures onto TSB agar plates containing 500 mg/ml of chloramphenicol (a concentration only permissive to nfxC mutants), supplemented or not with MC-207,110 (20 mg/ml). Plates containing this low concentration of the EPI showed a 50% reduction in the emergence of nfxC colonies after 24 h of incubation at 37uC when compared to control plates (data not shown), confirming the efficiency of this molecule in blocking the activity of MexEF-OprN. Thus, MC-207,110 was initially added to synchronized MGL01 cultures (OD 600 = 0.05) at a concentration of 20 mg/ml. To minimize growth inhibition effect caused by the EPI, a supplemental 20 mg/ml was added only after 2 h of growth. Twenty mg/ml of MC-207,110 has no effect on bacterial growth whereas 40 mg/ml results in a slight reduction of MGL01 growth (Fig. S4). For LC-MS/MS analyses, whole culture, supernatant and PBS washed cell samples were used.
Swarming and biofilm assays. Swarming motility and biofilm assays were performed as described previously [59,60]. Swarm plates were incubated at 30uC for 16 h. Biofilm assay was conducted at 37uC for 72 h using TSB for bacterial growth.