FimL Regulates cAMP Synthesis in Pseudomonas aeruginosa

Pseudomonas aeruginosa, a ubiquitous bacteria found in diverse ecological niches, is an important cause of acute infections in immunocompromised individuals and chronic infections in patients with Cystic Fibrosis. One signaling molecule required for the coordinate regulation of virulence factors associated with acute infections is 3′, 5′-cyclic adenosine monophosphate, (cAMP), which binds to and activates a catabolite repressor homolog, Vfr. Vfr controls the transcription of many virulence factors, including those associated with Type IV pili (TFP), the Type III secretion system (T3SS), the Type II secretion system, flagellar-mediated motility, and quorum sensing systems. We previously identified FimL, a protein with histidine phosphotransfer-like domains, as a regulator of Vfr-dependent processes, including TFP-dependent motility and T3SS function. In this study, we carried out genetic and physiologic studies to further define the mechanism of action of FimL. Through a genetic screen designed to identify suppressors of FimL, we found a putative cAMP-specific phosphodiesterase (CpdA), suggesting that FimL regulates cAMP levels. Inactivation of CpdA increases cAMP levels and restores TFP-dependent motility and T3SS function to fimL mutants, consistent with in vivo phosphodiesterase activity. By constructing combinations of double and triple mutants in the two adenylate cyclase genes (cyaA and cyaB), fimL, and cpdA, we show that ΔfimL mutants resemble ΔcyaB mutants in TM defects, decreased T3SS transcription, and decreased cAMP levels. Similar to some of the virulence factors that they regulate, we demonstrate that CyaB and FimL are polarly localized. These results reveal new complexities in the regulation of diverse virulence pathways associated with acute P. aeruginosa infections.


Introduction
Pseudomonas aeruginosa is an important human pathogen found throughout the environment in diverse ecological niches and is associated with severe opportunistic infections [1]. Patients with some degree of immunocompromise and epithelial barrier injury, such as those with severe burns or surgical incisions, on mechanical ventilation, or receiving cytotoxic chemotherapy, are particularly vulnerable to acute infection. This Gram-negative bacterium is also the leading cause of chronic pulmonary infections and death in Cystic Fibrosis patients. Even with appropriate medical treatment, mortality remains high and antibiotic resistance is increasingly common. Thus, it is of great importance to identify new potential therapeutic targets.
P. aeruginosa produces several virulence factors associated with acute infection, including components of the Type IV pilus (TFP), Type III secretion system (T3SS), Type II secretion system (T2SS), and quorum sensing systems (reviewed in [2]). TFP are polarly localized appendages composed of pilin polymers that undergo reversible assembly and disassembly and allow the bacteria to move over a solid surface in a process termed twitching motility (TM) (reviewed in [3]). In addition, TFP function as phage receptors, are initiators of biofilm formation, and are the primary adhesins associated with P. aeruginosa binding to both biotic and abiotic surfaces, including mammalian cells. The T2SS functions to secrete enzymes such as proteases, hemolysins and toxins into the extracellular milieu to cause host cell cytotoxicity. The T3SS, which resembles a molecular syringe, directly injects effector proteins, including ExoU, ExoS, ExoT, and ExoY, from the bacteria to the host cell cytosol. Components of related T3SS in Shigella flexneri and Yersinia pestis have been shown to localize to the cell pole [4,5]. These effector proteins have profound outcomes on host cell signaling, leading to host cell death and alterations in the host immune system. The bacterial molecules involved in the activation and coordination of virulence factor production include numerous signal transduction cascades [6].
One signaling molecule required for the coordinate regulation of virulence factors associated with acute infections is 39, 59-cyclic adenosine monophosphate, (cAMP). cAMP functions as a cofactor for the cAMP binding protein, variously known as CAP or CRP. Upon binding cAMP, CRP undergoes a conformational change and binds to specific DNA sequences, thus modulating gene transcription. cAMP signaling has also been shown to regulate a variety of processes in many bacteria, from iron uptake in E. coli [7] to competence in Haemophilus influenzae [8]. cAMP signaling in the regulation of the lac operon during diauxic growth [9] and in catabolite repression [10] has been extensively studied in Escherichia coli and other bacteria. More recently, cAMP signaling has been implicated in the regulation of virulence factors for many pathogenic firmicutes and proteobacteria, although the mechanisms vary widely [10].
In P. aeruginosa, cAMP is an allosteric regulator of the CRP homolog, Vfr (virulence factor regulator) [11]. In contrast to E. coli, cAMP signaling in P. aeruginosa is not involved in catabolite repression. Instead, Vfr controls the transcription of over 200 genes, including those associated with TFP, T3SS, T2SS, flagellarmediated motility, and quorum sensing systems [12,13]. The mechanism by which Vfr regulates all of these virulence factors is not entirely known. A consensus DNA binding sequence has been identified but does not fully explain every gene regulated by Vfr [14]. To add further complexity, recent data suggests that Vfr may control expression of some genes in a cAMP-independent manner [15]. cAMP levels are regulated by synthesis and degradation [16,17]. In P. aeruginosa, two adenylate cyclases have been described that synthesize cAMP. CyaB, a membrane bound adenylate cyclase, plays the major role in cAMP synthesis, while CyaA, a cytoplasmic adenylate cyclase, plays a minor role in synthesis [12,18]. Accordingly, CyaB is an important regulator of Vfr-dependent functions; DcyaB and Dvfr mutants exhibit attenuated virulence in a mouse model of acute pneumonia infection [19].
We previously carried out a transposon-mediated genetic screen to identify P. aeruginosa genes important for host cell cytotoxicity. This screen identified TFP, ExoU, and T3SS components as critical mediators of host cell death [20,21]. Subsequent studies have also shown that TFP and the T3SS are important virulence factors in mouse models of acute P. aeruginosa infections [20,22,23] and that the presence of a functional T3SS correlates with unfavorable clinical outcomes in acute human infections [24].
Our genetic screen also identified a protein, FimL, with homology to the N-terminus of a P. aeruginosa CheA homolog, ChpA [25]. In addition to the hybrid kinase ChpA, the Chp operon encodes two CheY homologs as well as other components of the chemosensory system and regulates pilin extension and retraction [26]. We demonstrated that FimL was important in regulating TFP function, production and secretion of T3SS associated virulence factors, biofilm formation, and concluded that FimL likely functions upstream of Vfr. Spontaneous extragenic suppressors of FimL, which arose at higher than expected frequencies, regained TM and T3SS-mediated cytotoxicity towards mammalian cells. One suppressor mutant was analyzed in greater detail and found to produce nearly ten-fold higher levels of cAMP compared to the parent strain [25] although the site of the extragenic mutation(s) remains unknown. Together, these results suggest that FimL and Vfr are components of intersecting pathways that serve to regulate diverse virulence factors involved in acute infections.
In this study, we performed genetic and physiologic experiments to further define the mechanism of action of FimL. In a transposon mutant screen designed to identify extragenic suppressors of FimL, we found a cAMP-specific phosphodiesterase, suggesting that FimL regulates cAMP levels. We measured cAMP levels in the DfimL mutant and found decreased levels of cAMP, similar to the diminished levels observed in DcyaB. By constructing informative double and triple mutants in cyaA, cyaB, cpdA and fimL, we show that DfimL mutants resemble DcyaB mutants with respect to altered TM, decreased T3SS transcription, and decreased cAMP levels. Epistasis experiments suggest that FimL function is CyaB dependent. Similar to some of the virulence factors regulated by cAMP, including TFP [27], flagella [28], and potentially the T3SS, FimL and CyaB are polarly localized. While this manuscript was in preparation, it was reported that FimL and other components of the Chp operon also modulate cAMP levels [29]. Together, these results suggest that the Chp system not only regulates pilin function but also coordinately regulates cAMPdependent pathways, including the T3SS. We hypothesize that FimL plays a pivotal role in this coordinate regulation by interfacing with the Chp chemosensory system, TFP, and the cAMP/Vfr pathway. Our studies reveal new complexities in the regulation of diverse virulence pathways associated with acute P. aeruginosa infections.

Bacterial Cultures and Assays
The bacterial strains and plasmids used in this study are described in Table S1. Bacteria were routinely streaked onto on Luria-Bertani (LB) 1.5% agar and grown in liquid LB overnight shaking at 250 rpm at 37uC. E. coli strains S17.1 and SM10 were used as donor strains with P. aeruginosa recipient strains in bacterial conjugation. After mating with E. coli, P. aeruginosa strains were selected by growth on 1.5% Difco Pseudomonas isolation agar (Becton Dickinson). Antibiotic concentrations used for E. coli, tetracycline, 5 mg/mL; ampicillin, 100 mg/mL; gentamicin, 10 mg/mL; kanamycin 50 mg/mL and for P. aeruginosa, tetracycline, 100 mg/mL, carbenicillin 250 mg/mL, gentamicin, 100 mg/ mL. For b-galactosidase experiments and cAMP assays, bacterial frozen stocks were freshly streaked onto LB agar, and following overnight growth were inoculated into 5 mL of MinS media [30] with or without addition of CaCl 2 to a final concentration of 2 mM. Following growth overnight, samples were processed.
b-galactosidase assays were performed as previously described [25].
cAMP assays were performed using the Cayman cAMP EIA assay kit. Briefly, a volume of culture corresponding to 10 OD600 units was pelleted, and resuspended in 500 mL 0.1 M HCl. Following 30 seconds of sonication, the debris was removed with a low-speed spin after 10 min at 1000 X G for PA103 or 14,000 X G for PAO1 strains. 400 mL of supernatant was transferred to a fresh tube containing an equal volume of EIA buffer (supplied with kit), and the samples were further processed according to the manufacturer's manual. For acetylated reactions, samples were processed as above, except that the 500 mL of supernatant (following sonication and pelleting) was acetylated according to manufacturer's protocol prior to dilution in EIA buffer and further processing.

Transposon Mutagenesis
The donor strain SM10-l pir containing pBT20 was mated with the recipient strain PAO1DfimL::CTXP exoT -lacZ. Transformants were plated on 1.5% LB agar plates containing bromochloro-indolyl-galactopyranoside (40 mg/mL) and gentamicin and screened for restoration of TM and blue colony color. Insertion sites were determined using semirandom PCR [31].
Twitching Motility assays were performed using the subsurface stab assay as previously described [26].

Plasmid construction
All plasmids were purified using Qiagen kits and standard molecular biology recombinant techniques were used. Enzymes were purchased from New England Biolabs and used as recommended by the manufacturer. All primers used in construction were designed using the PAO1 genome (www.pseudomonas. com) and synthesized by Qiagen or Elim Biopharmaceuticals, Inc. Primer sequences are available upon request.

Construction and complementation of in-frame deletion mutants
All matings were performed as described previously [26]. In general, a PCR fragment 1 kb upstream of the target deletion gene and 1 kb downstream was synthesized and overlap PCR performed to create the deletion PCR fragment. This fragment with created with unique restriction enzyme sites on both ends and cloned into pJB100T or pEX100T. This plasmid was then introduced into S17.1 E. coli strain and mated to P. aeruginosa. The vector backbone was then counter-selected using 5% sucrosecontaining 1.5% LB agar plates and the resulting unmarked deletion strain was checked with PCR and/or Southern blot.

Construction of CTX-exoT-lacZ strains
The promoter region (600 bp upstream of the exoT gene) was directionally cloned into the mini-CTX-lacZ vector as a HindIII-EcoR1 fragment, following amplification from genomic DNA with the primers 59AAGCTTCCACGCCTGACATCGCTCAC 39 and 59GAATTCGCCACGAAAGACGGGTTCTG 39. The resulting CTX-P exoT -lacZ plasmid was mated into wild type PAO1 to generate PAO1::CTXP exoT -lacZ. The DfimL derivative was generated by mating the DfimL allelic exchange plasmid pJEN36 into PAO1::CTXP exoT -lacZ. The Dvfr derivative was generated by mating pJEN51 into PAO1::CTXP exoT -lacZ. PA103 versions of these strains were also generated with these plasmids.

Construction of chromosomal and plasmid-borne FimL3X-FLAG strains
A C-terminal triple FLAG-tagged version of fimL, containing a diagnostic Cla1 restriction site was constructed as follows. Using primers 59CGGGATCCCCGGCCCGGCCAGCCATAGCAG-CAGGGGG-39 and 59CCATCGATTTTATCGTCATCGTCT-TTGTAGTCGGCGGCTTTATCGTCATCGTCTTTTAGTC-GGCGGCCACCGGCAGTCCGA -39, a 3 kb fragment containing a BamH1 site followed by 1 kb of sequence upstream of fimL, the fimL coding region immediately upstream of the stop codon, and 2 FLAG tags followed by Cla1 site was amplified. Separately, primers 59CCATCGATGACTACAAAGATGACGATGACAA-ATGATGGCCGGCGAGTTCCGCTGGC-39 and 59-GCTCA-GACGGAGCGTTCTGGACCGTGACCTC-39 were used to amplify a fragment containing a Cla1 site, followed by a single FLAG tag, the stop codon, and 1 kb of sequence downstream of fimL, terminating with an Xba1 site. The amplicons were digested respectively with BamH1 and Cla1, and Cla1 and Xba1, ligated together into pOK12 that had been digested with BamH1 and Xba1. The resulting triple FLAG-tagged fimL fragment was excised with Spe1 and cloned into the allelic exchange vector pJB100T. The resulting plasmid pJTW019 was mated into PAO1DfimL::CTXP exoT -lacZ. The complemented fimL mutant was confirmed to carry the triple flag-tagged fimL by PCR/Cla1 digest, and functional complementation was confirmed by Western blot using an anti-Flag monoclonal antibody (Sigma). The insert from pJW019 was subcloned as a Spe1 fragment into the Xba1 site of pUCP19Dlac. A BamH1 digest was performed to identify which clones were in the correct orientation to be expressed from the pTac promoter. The resulting plasmid pUCP19-fimL3X-FLAG was transformed into P. aeruginosa strains (see Table S1); expression from the plasmid was confirmed by Western blot.

Construction of FimL-GFP strains
fimL was PCR amplified using 59 TGGGCTAGCGAATT-CATGGTCACAGGAGCC and 59 GGACTGCCGGTGGCC-GCCGGCGGCGGCAAGCTTGTGAGCAAG with a 3XGly linker, minus the STOP codon and cloned into pMBAD-GFP to generate pYFI007. fimL-GFP was subcloned from pYFI007 into PJB100T by overlap PCR with a PCR fragment downstream of fimL on the PAO1 chromosome and was amplified using primers 59 CGGCATGGACGAGCTGTACAAGTAATGGCCGGCG-AGTTCCGCTGGC and 59CAGGGTAATACTAGTAGCG-GCGCGCCAGGTAC to generate the fimL-GFP gene replacement construct pYFI043. Similarly, cyaB was amplified using 59 GCTAGCGAATTCATGAAGCCTACCCTCCCCGACC 39 and 59 CCCCGGGTACCGCCGCCGCCGAGGATGACCTTGT-CGCGCAGG39 to generate pYFI184.
SDS-PAGE and immunoblotting assays were performed as in [32].

Results
A screen for extragenic suppressors of fimL identifies the phosphodiesterase CpdA To further understand how FimL controls virulence pathways, we devised a genetic screen to identify new regulators in the FimL pathway. Our goal was to find single gene disruptions that resulted in restoration of TFP function and T3SS function in a DfimL mutant. Therefore, we screened a library of transposon insertions in a DfimL strain for mutants that simultaneously regained TM and T3SS function. Specifically, we utilized a mariner transposon [33] to mutagenize PAO1DfimL containing an E. coli lacZ gene reporter fused to the promoter of the T3SS effector exoT (denoted P exoT -lacZ). Approximately 100,000 transposon mutants (approximately 20X genome coverage) were visually screened on LB plates containing bromo-chloro-indolyl-galactopyranoside (X-gal) and isopropyl b-D-1-thiogalactopyranoside (IPTG) for simultaneous recovery of exoT transcription (as evidenced by blue colonies) and TFP function (as evidenced by TM, a phenotype readily visible as a colony with an expanding edge). From this screen, we recovered a transposon insertion in PA4969, which regained P exoT -lacZ expression and TM in the DfimL background. We constructed an in-frame deletion of PA4969 in PAO1DfimL (PAO1DfimLDcpdA) and confirmed that inactivation of PA4969 was responsible for suppressing the DfimL phenotype. PAO1DfimLDDcpdA showed high P exoT -lacZ expression (Fig. S1A) and partially restored TM (Fig. S1B). During the preparation of this manuscript, PA4969 was characterized as a cAMP-specific phosphodiesterase, CpdA [34].

FimL regulates cAMP levels
Since disrupting cpdA restored TM and T3SS function in the DfimL mutant, we predicted that DfimL mutants would have decreased levels of cAMP. To test this hypothesis, we measured bacterial cAMP. The experiments were performed in the presence and absence of calcium, since minimal media lacking calcium induces expression of T3SS gene expression [35]. We previously reported that cAMP levels were barely detectable in the wild type PAO1 strain [25], however with protocol changes we were able to reproducibly measure cAMP in PAO1. In the absence of calcium, we observed an ,80% decrease in cAMP levels in the DfimL mutant compared to PAO1 ( Fig. 1A; P,0.001). As controls, we assayed PAO1DcyaA, PAO1DcyaB, and the double mutant PAO1DcyaADcyaB. No significant difference was observed in cAMP levels in the DcyaA strain when compared to wild type. However the DcyaB and DcyaADcyaB mutants showed an ,85% and ,95% decrease, respectively, in cAMP levels when compared to wild-type (P,0.001). The low cAMP levels observed in PAO1DcyaB, PAO1DfimL and PAO1DcyaADcyaB were not statistically different from each other. Together, these results suggest that FimL modulates cAMP levels.
Deletion of the phosphodiesterase gene (PAO1DcpdA) resulted in an approximately 100-fold increase in cAMP levels compared to wild type (Fig. 1A). Thus we reasoned that we could detect changes in cAMP levels very robustly in a sensitized mutant background in which cpdA was inactivated. We therefore constructed in-frame deletions of fimL, cyaA, and cyaB in the phosphodiesterase mutant background PAO1DcdpA and measured cAMP levels. As shown in 1B, deletion of fimL (PAO1DcpdADfimL) or cyaB (PAO1DcdpADcyaB) resulted in ,50% (P,0.001) and ,75% (P,0.001) decrease in cAMP levels, respectively, compared to PAO1DcdpA, while no statistically significant decrease was observed in the cyaA mutant (PAO1DcpdADcyaA). cAMP was undetectable in the PAO1DcpdADcyaADcyaB mutant, confirming that CyaA and CyaB are the only source of cAMP in this strain. Altogether, the trends in cAMP levels in PAO1 or the PAO1DcpdA background are similar, lending further support to our conclusion that FimL regulates cAMP levels.
To ascertain if these results were strain-specific, we constructed in-frame deletion mutants in PA103. We reproducibly detected ,10-fold decrease in cAMP levels in PA103DfimL and PA103 DcyaB compared to PA103 ( Fig. 1C; P,0.001). The levels of cAMP were restored to near wild type levels in the PA103DfimL strain complemented with FimL-3X-FLAG at the endogenous locus (Fig. S2). As expected, in PA103DcyaA, cAMP levels were indistinguishable from wild type PA103, and cAMP was undetectable in the PA103DcyaADcyaB mutant (Fig. 1C). Thus, deletion of fimL correlates with a decrease in cAMP levels in both PAO1 and PA103, strongly supporting a role for FimL in cAMP production in P. aeruginosa.

FimL promotes CyaB-dependent cAMP synthesis
FimL regulation of cAMP could occur by affecting cAMP synthesis or by stimulating degradation of cAMP via the phosphodiesterase CpdA. If FimL only functions through CpdA activity, then cAMP levels should not change between the DcpdA and the DcpdADfimL mutants. As shown in Fig. 1B, cAMP levels are reduced in PAO1DcpdADfimL compared to PAO1DcpdA, suggesting that FimL regulates cAMP independent of CpdA.
We therefore considered the alternative model that FimL regulates cAMP biosynthesis and compared cAMP levels in the presence or absence of FimL in PAO1DcyaA, PAO1DcyaB, and PAO1DcyaADcyaB (Fig. 1A). cAMP levels decreased ,90% in PAO1DcyaADfimL compared to PAO1DcyaA (P,0.001). In contrast, there was no statistically significant difference in cAMP levels between PAO1DcyaB and PAO1DcyaBDfimL or between PAO1DcyaADcyaB and PAO1DcyaADcyaBDfimL. In the DcyaADfimL mutant, CyaB is the only source of cAMP; thus, the decrease in cAMP levels in this mutant compared to the DcyaA mutant suggests that FimL affects cAMP synthesis via CyaB. The finding that cAMP levels are indistinguishable between DcyaBDfimL and DcyaB also suggests that FimL does not act through CyaA. Similar results were observed with deletion mutants in the PAO1DcpdA background (Fig. 1B).

FimL does not affect cyaB transcription or protein levels
Our results so far suggest that FimL modulates cAMP levels through CyaB. This regulation could occur by modulating cyaB transcription, CyaB protein levels, or CyaB activity. To determine whether cyaB expression was altered at the transcriptional level, we compared the b-galactosidase activity of the lacZ gene fused to the promoter of cyaB and integrated into the chromosome at the CTX site in PAO1 and PAO1DfimL. As shown in Fig. 2, there was no difference in transcription from the cyaB promoter in the wild type or the DfimL background. Minimal transcription of the lacZ reporter was observed in the absence of the cyaB promoter. In contrast to strain PAK in which cyaB transcription was reported to be calcium dependent in microarray analysis [12], we did not observe calcium-dependent cyaB transcription in PAO1.
We next assayed CyaB protein production in a strain in which the native cyaB gene was replaced with a cyaB-His fusion. As shown in Fig. 2B, protein levels of CyaB-His were unaltered in PAO1DfimL. We therefore conclude that FimL modulates CyaB function at a post-translational step.

Expression of T3SS genes correlates with intracellular cAMP levels
We previously published that fimL mutants exhibited decreased ExoT production and secretion [25]. vfr mutants were also shown to be defective in T3SS function [12]. Together with published data and given our cAMP results, we predicted that fimL mutants would show decreased transcription of T3SS genes. We used our panels of isogenic mutants to directly assess the role of FimL and cAMP on the transcription of a representative T3SS gene, the secreted toxin ExoT. This readout is robust and can readily be detected in either PA103 or PAO1.
A transcriptional fusion comprising the exoT promoter fused to lacZ (P exoT -lacZ) was integrated at the CTX phage attachment site in DfimL, DcyaA, and DcyaB mutants in PAO1 (Fig. 3A) and PA103 (Fig. 3B) and assayed for b-galactosidase activity in the presence or absence of calcium. We reproducibly found a correlation between cAMP levels and P exoT -lacZ transcription. Compared to wild type PAO1 or PA103, P exoT -lacZ transcription was unaffected in the PAO1DcyaA mutant or PA103DcyaA mutant, respectively. In contrast, the corresponding DfimL and the DcyaB mutants showed greatly reduced P exoT -lacZ expression, similar to the levels seen in the Dvfr and DcyaADcyaB mutants. P exoT -lacZ transcription in PAO1DfimL and PA103DfimL was restored to wild type levels upon complementation with fimL (Figs. 3A and 3B).

TM correlates with cAMP levels
Assembly and function of TFP requires over 40 gene products whose transcription is regulated in part by Vfr [3], therefore we predicted that cAMP would also regulate TM. We measured TM of DcyaA, DcyaB, DfimL and Dvfr mutants in PAO1 and PA103 by the subsurface stab assay. All mutants that exhibited decreased cAMP levels ( Fig. 1) and decreased P exoT -lacZ expression (Figs. 3A and 3B) also showed reduced TM, including DfimL, DcyaB, DcyaADfimL, DcyaBDfimL, and DcyaADcyaB mutants in both the PAO1 (Fig. 3C) and PA103 (Fig. 3D) backgrounds. TM was restored to the wild type diameter upon complementation of the corresponding DfimL mutant.

Ectopic expression of fimL reduces cAMP levels
We tested the effect of ectopic expression of fimL on cAMP levels and cAMP-dependent phenotypes. FimL-FLAG was cloned into a plasmid under the control of a constitutive promoter (pUCP19Dlac), denoted ''pfimL-FL'' and introduced into PAO1, PAO1DcpdA, and PA103. We confirmed that the addition of the epitope tag did not affect FimL function by demonstrating that fimL-FL restored cAMP production in PAO1DcpdA (Fig. 1B) and in PA103DfimL (Fig. S2). Expression of the empty vector had no effect on cAMP levels; however, expression of pfimL-FL resulted in decreased levels of cAMP in PAO1DcpdA and PAO1DcpdADcyaA compared to the corresponding isogenic strain containing the control vector (Fig. 4A). Similar results were observed when pfimL-FL was introduced into PA103 (Fig. 4B) and PAO1 (data not shown) suggesting that the decreased cAMP levels upon ectopic expression of fimL are not strain-specific. Expression of pfimL-FL did not affect P cyaB -lacZ expression (Fig. S3), indicating that the inhibitory effect of FimL is not through cyaB transcription. Expression of pfimL-FL in PAO1DcyaB or PA103DcyaB did not significantly change the already low levels of cAMP in these strains.
Given these findings, we predicted that ectopic expression of fimL would also affect TM and transcription of T3SS genes. As shown in Fig. S4, ectopic expression of pfimL-FL inhibited TM in PAO1. Likewise, ectopic expression of pfimL-FL decreased P exoT -lacZ expression in PAO1 (Fig. 5A) and PA103 (Fig. 5B) and in the corresponding DcyaA mutants. As expected from previous experiments, ectopic expression of pfimL-FL in PA103DcyaB did not further decrease the already low levels of P exoT -lacZ transcription, supporting the hypothesis that the regulation of cAMP by FimL is CyaB-dependent.
Together, these results suggest that precisely tuned levels of FimL are critical to modulate cAMP levels. It is possible that in order to promote cAMP synthesis efficiently, the stoichiometry of FimL:CyaB is critical. However, FimL is not absolutely required for CyaB function, as some cAMP is synthesized in the absence of FimL (Fig. 1). Consistent with these observations, we found that expression of pcyaB can complement the PAO1DfimL mutant for T3SS function (Fig. S5). We propose that FimL is required for optimal CyaB adenylate cyclase activity.

FimL and CyaB are polarly localized
Many of the virulence factors that are regulated by FimL, such as TFP and potentially the T3SS, are polarly localized in Enterobacteriaceae. We therefore investigated the localization of FimL and CyaB. For our initial studies, we constructed a plasmidborne fimL-GFP fusion under control of an arabinose inducible promoter (pfimL-GFP) and introduced the plasmid into PAO1 or PAO1DfimL. In the absence of arabinose induction, low-level expression of fimL-GFP was sufficient to restore TM in PAO1DfimL (Fig. S6A), confirming that fusion to GFP did not interfere with FimL function. When fimL-GFP was induced with arabinose, TM and cAMP levels were inhibited compared to the control strain carrying a GFP-expressing plasmid (PAO1-pGFP) (Figs. S6A and S6B). Fluorescence microscopy of log-phase PAO1DfimL+pfimL-GFP grown in the presence (data not shown) or absence of arabinose (Fig. 6D) revealed that FimL-GFP localized to both poles of the cell. Images of stationary phase PAO1DfimL+pfimL-GFP induced with arabinose show an apparent decrease in the frequency of polarly localized puncta (Fig. 6E), suggesting that overexpression of fimL leads to FimL protein delocalization and subsequent interference with function. Since overexpression of GFP fusion proteins can lead to artifactual localization [36], we replaced the native fimL locus with fimL-gfp. Expression of fimL-GFP from a single copy gene at the native locus restored TM and cAMP production (Figs. S6A and S6B) and exhibited polar localization regardless of growth phase (for representative images see Figs. 6A and 6B).
To examine whether CyaB is also polarly localized, we generated the strain PAO1DcyaB + pcyaB-GFP. With arabinose induction, TM was restored to wild type levels (Fig. S6C), and microscopy shows bipolar localization of CyaB-GFP and a strong signal around the perimeter of the cell in the absence (data not shown) or the presence of arabinose (Fig. 6F) from log-phase grown cells. Together, these results suggest that both FimL and CyaB are polarly localized.
Discussion cAMP is increasingly appreciated as an important regulator of diverse pathways in many bacteria. In P. aeruginosa, it is involved in the coordinate regulation of many critical virulence factors. CyaB, and a cAMP-dependent transcriptional regulator, Vfr, regulate the expression of over 200 genes, including components of the T3SS, TM, the T2SS, flagellar motility, and quorum sensing [12]. Through genetic and physiologic analyses, we demonstrate that FimL, a protein with two histidine phosphotransfer (Hpt) like domains, is required for optimum CyaB-dependent biosynthesis of cAMP. Additionally, we find that both FimL and CyaB are polarly localized, suggesting that spatial regulation of cAMP production may be important to the virulence of P. aeruginosa. Overexpression of fimL inhibits cAMP production and may cause FimL to mislocalize. We note that Fulcher et al. found that a disruption in fimL decreased cAMP levels in the P. aeruginosa strain PAK [29], suggesting this function in relevant across Pseudomonad species.
How might FimL contribute to cAMP production? Our experiments eliminate the possibility that FimL is required to maintain protein levels of CyaB in PAO1 or PA103, as no decrease in cyaB transcription or CyaB protein levels were observed in fimL mutants. Another possibility is that FimL enhances the adenylate cyclase activity of CyaB. In support of this hypothesis, epistasis experiments suggest that FimL and CyaB function in the same pathway. Our finding that both FimL and CyaB are polarly localized indicates that they may function as a complex. A simple hypothesis is that FimL is required for CyaB localization and/or CyaB is required for FimL localization. However, preliminary experiments suggest that their interactions may be more complicated, as FimL is still polarly localized in DcyaB mutants, and CyaB is polarly localized in the DfimL mutant (unpublished data). Interestingly, we have recently reported that CbpA, a cAMP binding protein of unknown function, is also localized to the poles [37]. An attractive possibility is that localized regulation of cAMP is important in regulating polar proteins. Indeed, spatial gradients of another second messenger signaling molecule, c-di-GMP, which is important for biofilm formation and swarming motility, have been recently reported [38]. FimL shares homology with the N-terminal 563 amino acids of the 2477 amino acid ChpA protein, and it is tempting to speculate that binding interactions between FimL and ChpA or ChpA binding partners are important for regulating CyaB and/or other components of the cAMP regulatory circuit. ChpA is one of the most complex CheA homologs yet described, with 8 potential Hpt domains and a CheY-like domain [26]. Work from several labs suggests that the histidine kinase domain, the CheY domain, and at least two of the Hpt domains, are required for TM in PAO1 [32,39]. Notably, glutamine (rather than histidine) is found at the two putative FimL phosphoryl group acceptor sites; hence it is unlikely that FimL is capable of phosphotransfer in a canonical manner. One possibility is that FimL, ChpA, and CyaB form a polarly localized complex to allow spatial, environmental, and temporal production of cAMP that regulates multiple polarly localized structures, including TFP and the T3SS (see Fig. 7).
ChpA is thought to be the central histidine kinase of a larger regulatory circuit with homology to chemosensory signal transduction systems. This module includes PilJ, a putative methylaccepting chemotaxis protein; PilK, a methyltransferase homolog, 2 CheW homologs (PilI and ChpC); ChpB, a methylesterase homolog; and two additional CheY-like response regulators (PilG and PilH). PilJ, PilK, PilI, PilG, and PilH are required for TM. Fulcher and co-workers recently reported that disruption of chpA, pilJ, or pilG decreased cellular cAMP levels through a CyaBdependent mechanism, whereas loss of pilH resulted in increased cAMP levels [29]. In conjunction with our finding that FimL also promotes cAMP production in a CyaB-dependent manner (this work) and that overexpression of either fimL (this work and [26]) or chpA [26] impairs cAMP-dependent pathways such as TM, we  . FimL may function to link the Chp system and CyaB to regulate cAMP levels in P. aeruginosa. FimL may interact with ChpA and/or CyaB to affect cAMP biosynthesis activity directly or indirectly. The Chp chemosensory system is thought to regulate pilus extension and retraction via two-component signaling. An input signal to the methyl-accepting chemotaxis protein PilJ induces autophosphorylation of the hybrid histidine kinase ChpA. PilG and PilH are putative response regulators that accept phosphoryl groups from phosphorylated ChpA and regulate pilus function. CpdA is a phosphodiesterase that degrades cAMP. cAMP is an allosteric regulator of Vfr which regulates multiple virulence pathways. OM and IM refer to the outer membrane and inner membrane respectively. doi:10.1371/journal.pone.0015867.g007 propose that FimL could link the Chp system and CyaB activation to promote cAMP synthesis (Fig. 7). This interaction may involve direct interactions between FimL and ChpA. Because ChpA contains 8 potential Hpt domains, there are many potential activation states that ChpA might occupy, allowing fine-tuning of response to environmental stimuli and subsequent downstream signaling. Interactions between FimL and ChpA could influence ChpA activity and affect downstream ChpA signaling.
An important strength of our studies is that we verified critical observations in two different strains. Our work demonstrates that in PAO1 and PA103, the cAMP defects in the DfimL and DcyaB mutants translate to defects in the Vfr-cAMP mediated functions, including decreased T3SS transcription and TFP-mediated motility. This finding contrasts the reported near wild type TM of PAKDcyaB [12] and PAKDfimL [25] despite the decreased cAMP levels in these mutants [29]. These differences suggest that additional or different factors may regulate TM in the PAK strain background. In addition, whereas cyaB transcription is reported in PAK to be induced under low calcium conditions [12], we observed that transcription and protein levels of CyaB were similar under calcium-poor and calcium-replete conditions.
We note some subtle differences between PAO1 and PA103 with respect to cAMP-mediated functions. P exoT -lacZ transcription was relatively insensitive to calcium in PA103, even though production and secretion of the T3SS effectors is induced in calcium-poor media. In contrast, P exoT -lacZ transcription in PAO1 was induced in media lacking calcium. These differences may reflect strain-specific fine-tuning of virulence circuits.
In summary, using a genetic screen and physiologic studies, we provide evidence that FimL regulates CyaB activity and cAMP production at a post-translational step and that both proteins exhibit polar localization. Future studies will be directed at determining the mechanism and role of the polarly localized cAMP biosynthesis and spatial regulation of virulence circuits.