Effect of the Gene doublesex of Anastrepha on the Somatic Sexual Development of Drosophila

Background The gene doublesex (dsx) is at the bottom of the sex determination genetic cascade and is transcribed in both sexes, but gives rise to two different proteins, DsxF and DsxM, which impose female and male sexual development respectively via the sex-specific regulation of the so-called sexual cyto-differentiation genes. The present manuscript addressed the question about the functional conservation of the tephritid Anastrepha DsxF and DsxM proteins to direct the sexual development in Drosophila (Drosophilidae). Methodology To express these proteins in Drosophila, the GAL4-UAS system was used. The effect of these proteins was monitored in the sexually dimorphic regions of the fly: the foreleg basitarsus, the 5th, 6th and 7th tergites, and the external terminalia. In addition, we analysed the effect of Anastrepha DsxF and DsxM proteins on the regulation of Drosophila yolk protein genes, which are expressed in the fat body of adult females under the control of dsx. Conclusions The Anastrepha DsxF and DsxM proteins transformed doublesex intersexual Drosophila flies into females and males respectively, though this transformation was incomplete and the extent of their influence varied in the different sexually dimorphic regions of the adult fly. The Anastrepha DsxF and DsxM proteins also behaved as activators and repressors, respectively, of the Drosophila yolk protein genes, as do the DsxF and DsxM proteins of Drosophila itself. Finally, the Anastrepha DsxF and DsxM proteins were found to counteract the functions of Drosophila DsxM and DsxF respectively, reflecting the normal behaviour of the latter proteins towards one another. Collectively, these results indicate that the Anastrepha DsxF and DsxM proteins show conserved female and male sex-determination function respectively in Drosophila, though it appears that they cannot fully substitute the latter's own Dsx proteins. This incomplete function might be partly due to a reduced capacity of the Anastrepha Dsx proteins to completely control the Drosophila sexual cyto-differentiation genes, a consequence of the accumulation of divergence between these species resulting in the formation of different co-adapted complexes between the Dsx proteins and their target genes.


Introduction
Sex determination is the commitment of an embryo to either the male or female developmental pathway. A plethora of sex determination mechanisms exists; all of which are represented in insects [1,2,3]. In Drosophila melanogaster, the sex determination mechanism has been thoroughly analysed. The epistatic relationships between the sex determination genes in this species show that hierarchical interaction occurs among them (reviewed in [4]). The characterisation of these genes has shown that their control during development is governed by the sex-specific splicing of their products. The product of one gene controls the sex-specific splicing of the pre-mRNA from the downstream gene in the genetic cascade. Sex-lethal (Sxl) is at the top of this cascade; its product controls the splicing of its own pre-mRNA as well as the splicing of the pre-mRNA from the downstream gene transformer (tra). The Tra product and the product of the constitutive gene transformer-2 (tra-2) control the sex-specific splicing of pre-mRNA from the gene doublesex (dsx), which is transcribed in both sexes but gives rise to two different proteins, DsxF and DsxM. These are transcription factors that impose female and male sexual development respectively via the sex-specific regulation of the socalled sexual cytodifferentiation genes.
The gene dsx of Anastrepha species is transcribed during development and in adult life in both sexes, but its primary transcript undergoes sex-specific splicing so that a different mRNA is produced in each sex. These mRNAs encode the female DsxF and male DsxM proteins; these have the amino-terminal region in common but differ in the carboxyl-terminal region. The comparison of Aodsx mRNA molecular organisation in males and females suggest that, in Anastrepha, the male-splicing pathway represents the default mode. The conceptual translation of the male and female Anastrepha dsx mRNAs shows that they encode two polypeptides of 397 and 319 amino acids respectively. Their comparison with the Dsx proteins of other insects shows that the degree of similarity is higher for the female-specific than for the non-sex specific and the male-specific regions. Particularly conserved are the OD1 and OD2 domains, which endow the Dsx proteins with the capacity to interact with other proteins and with DNA [18,19].
Molecular evolutionary analysis (both at the nucleotide and amino acid levels) of dsx in different insects revealed a topology in good agreement with their owners' taxonomic relationships. The great majority of the nucleotide changes detected in the dsx gene of the analysed species were significantly synonymous, evidence that strong purifying selection has acted on dsx so that the functional structure of the Dsx proteins is preserved. Yet, the common region of DsxF and DsxM proteins appeared to be the main target for selection acting upon the long-term evolution of gene dsx. Although in a lesser extent if compared with common regions, the sex-specific segments of DsxF and DsxM proteins are also subject to purifying selection, as expected since they endow these proteins with a different, oppose transcriptional role that would be preserved across species [14].
Anastrepha obliqua dsxF-cDNA and dsxM-cDNA encoding the putative full-length DsxF and DsxM proteins respectively were introduced into Drosophila melanogaster, and their effect on somatic sexual development in the ensuing transgenic flies recorded. The Anastrepha DsxF and DsxM proteins allowed partial female and male sexual determination respectively in Drosophila. However, the extent of their influence was not the same in the different sexually dimorphic regions of the adult fly.

Results
The DsxF and DsxM proteins of Anastrepha supply partial female and male sexual determination function, respectively, in transgenic Drosophila flies To analyse the effect of the Anastrepha dsx gene in Drosophila, the GAL4-UAS system was used. AodsxF-cDNA and AodsxM-cDNA was linked to UAS sequences. As expected, none of the Aodsx transgenic Drosophila lines expressed the corresponding transgene in the absence of GAL4. If any basal expression existed, this would be irrelevant since XX and XY flies with one or two doses of each transgene are normal, fertile females and males respectively.
A set of different GAL4 driver lines was used to express the transgenic AoDsx proteins Tub-GAL4, Arm-GAL4 and C68a-GAL4 [20]. The first two drives expression ubiquitously whilst the latter one is specific for imaginal discs. It was found that, independent of the GAL4 driver used, the expression of either AoDsxF or AoDsxM proteins was lethal to the transgenic flies when these were raised at 25uC (both males and females died at the embryonic and early larval stages). This lethality was not suppressed in those transgenic flies lacking the endogenous dsx function, i.e., mutants for dsx (data not shown). A similar result was reported when the proteins of Drosophila [21] or Ceratitis capitata [12] DsxM protein were expressed in Drosophila transgenic flies. The effectiveness of the GAL4-UAS system depends on temperature: lower temperature reduces the effectiveness of GAL4 so that the expression of the UAS-transgene is reduced. Therefore, the transgenic flies were raised at either 18 or 22uC, although only some of the transgenic flies expressing either the AoDsxF or the AoDsxM proteins survived to adulthood (see Tables 1 and 2). These were processed as explained in Materials and Methods, so that the effect of the transgenic proteins on Drosophila somatic sexual development could be studied. To this end, the following sexually dimorphic regions of the fly were monitored: the foreleg basitarsus, the 5th, 6th and 7th tergites, and the external terminalia. In all cases, the control refers to either XX or XY dsx mutant flies and experiment refers to their XX sisters or XY brothers mutant for dsx but expressing the Anastrepha Dsx proteins. In wild type flies, the foreleg basitarsus contains several transversal rows, the last one forming the sex comb structure in males (SC in Figure 1A); this is absent in females. The sex comb is composed of dark, thick bristles, and is rotated to lie parallel to the proximal-distal leg axis. In XX and XY flies mutant for dsx, no sex comb is formed and the last transversal row of bristles (LTRB in Fig. 1B,C,D,E,F) is partially rotated and formed by bristles that are not as dark and thick as the sex comb bristles. In the present work, the phenotype was the same when dsx mutant XX and XY flies expressed either the MAo or the FAo transgene. See examples of control FAo#10/+; dsx 1 /dsx 1 ( Figure 1C) and its experimental sister FAo#10/+;arm-GAL4/+; dsx 1 /dsx 1 ( Figure 1D), non-expressing and expressing the transgene respectively, and control MAo#10/+; dsx 1 /dsx 1 ( Figure 1E) and its experimental brother MAo#10/+;arm-GAL4/+; dsx 1 /dsx 1 ( Figure 1F), non-expressing and expressing the transgene respectively. This suggests that neither the DsxF nor the DsxM proteins of Anastrepha have an effect on the sexual development of the Drosophila foreleg basitarsus. In the case of C68a-GAL4 driver (specific for imaginal discs) some survivors presented forelegs with morphological abnormalities (including partial duplications), whose occurrence is characteristic of cell death during development (data not shown).
The 5th and 6th tergites of wild type males are fully pigmented ( Fig. 2A) whereas in females only the posterior area is pigmented (Fig. 2B). In XX and XY flies mutant for dsx, the 5th tergite is intersexual and characterised by the presence of pigmented spots in the anterior area. The 6th tergite shows the male-like dark pigmentation. In our dsx 1 mutant stock, the 5th tergite showed a more male-like colouring, with only small, non-pigmented spots in the most anterior and lateral regions (see FAo#10/+; dsx 1 /dsx 1 in Fig. 2C). Usually, In transgenic flies mutant for dsx and expressing either the MAo or FAo transgenes, the 5th and 6th tergites showed a slight sexual transformation towards male or female respectively (see FAo#10/+;arm-GAL4/+; dsx 1 /dsx 1 in Fig. 2D, which shows a larger non-pigmented area in the most anterior region that is marked by a dotted line). This indicates that these transgenes had a small effect on the development of 5th and 6th tergites of dsx mutant flies.
The 7th tergite is present in wild type females and in dsx mutant XX and XY flies, although in these it is smaller. In the present work, this tergite could be still smaller in dsx mutant flies expressing the MAo transgene ( Figure 3F), whereas in dsx mutant flies expressing the FAo transgene a more female-like development could be observed ( Figure 3D).
The most conspicuous sexually dimorphic region of the fly is the external terminalia (Fig. 3A,B), which are derived from the genital disc (reviewed in [22,23]). This is composed of two genital primordia plus the anal primordium. In both sexes, only two of these primordia develop to form the adult terminalia. The anal primordium develops in both sexes but, depending on the genetic sex, will form either male or female analia. However, only one of the genital primordia develops in each sex, forming either the male or the female adult genitalia. This depends on the genetic sex of the fly, i.e., the production of either female DsxF or male DsxM protein. In loss-of-function dsx mutant flies -whether XX or XYboth genital primordia develop giving rise to variable intersexual terminalia with incomplete male and female genital structures and intersexual analia [24,25,26] (see Fig. 3C,E). Table 1 shows the results corresponding to the effect of the FAo transgene on the development of the external terminalia of dsx mutant XX and XY flies. The loss-of-function dsx 1 mutation used in this study caused a slightly different degree of intersexuality in XX and XY flies (Fig. 3C, E). This can be appreciated by comparing rows 1 and 3 of Table 1; male genital structures were more common and larger in XY than in XX flies mutant for dsx 1 . For this reason, the effect of the transgene was compared between XX sister flies, or between XY brother flies, expressing (or not) the transgene. The expression of the Anastrepha DsxF protein in both dsx mutant XX and XY flies caused a female transformation of the intersexual terminalia, though this was incomplete. In the case of the flies expressing the FAo#2 transgene and reared at 18uC (cross I, Table 1), the expression of this transgene determined an increase of the size of the female vaginal plates and a reduction in the size of the male genital arch, lateral plate and clasper structures. This transformation towards female sex is more evident in the case of the FAo#10 transgene in dsx mutant XX flies reared at 22uC (cross II, Table 1) (Fig. 3D). Both the frequency and the size of the male genital structures decreased. Their XY brothers did not survive, probably because the FAo#10 transgene is located on the X chromosome (and therefore dose compensated), and thus males express larger amounts of transgenic DsxF protein -which is lethal, as explained at the beginning of this section. It may also well be that the GAL4 driver line arm-GAL4 is more active than C68a-GAL4. The female sexual transformation was also manifested by the presence of female spermathecae (absent in dsx mutant flies) in some transgenic flies expressing either the FAo#2 or FAo#10 transgenes, and by a large reduction in the size of the penis apparatus.  Table 2 shows the effect of the MAo transgene on the development of the external terminalia of dsx mutant XX and XY flies. The expression of the Anastrepha DsxM protein in both dsx mutant XX and XY flies caused male transformation of the intersexual terminalia, though this was incomplete. As a control, the intersex phenotype of dsx 1 /dsx 1 flies of cross I (Table 1) was  used. The size of the female vaginal plates decreased whereas the size of the male genital arch, lateral plates and claspers increased. In some cases, neither T8 nor the vaginal plates were present (Fig. 3F).
Collectively, the morphological analyses suggest that the Anastrepha DsxF and DsxM proteins cause feminisation and masculinisation respectively of dsx intersexual Drosophila flies, though this transformation was incomplete and the extent of their influence varied in the different sexually dimorphic regions of the adult fly, suggesting that the partial sex-determination function of the Anastrepha Dsx proteins in Drosophila reflect a reduced capacity of the Anastrepha Dsx proteins to completely control the Drosophila sexual cyto-differentiation genes in the different sexually dimorphic regions.
The Anastrepha DsxF and DsxM proteins counteract the function of Drosophila DsxM and DsxF respectively An indistinguishable intersexual phenotype is attained when both DsxF and DsxM are either absent or simultaneously present whenever they are in similar amounts. If one of the Dsx proteins is in greater quantity, it determines the sexual development that the zygote will follow [27,28]. This is so because DsxF and DsxM behave as antagonistic transcriptional factors in the regulation of their common target genes (reviewed in [4]). We were interested in studying the capacity of the Anastrepha DsxF protein to compete with the endogenous Drosophila DsxM protein. To examine this, XY brothers expressing two FAo transgenes (FAo#2 and FAo#1) and carrying either two doses (dsx + / dsx + ) or one dose (dsx 1 / dsx + ) of the endogenous Drosophila dsx gene were produced (cross III, Table 1). These express the same amount of Anastrepha DsxF protein and either the normal amount or half the amount of Drosophila DsxM protein respectively. The XY transgenic flies with two doses of endogenous dsx (row 7, Table 1) showed some female genital structures such as T8 tergite and vaginal plates as well as a reduction of the penis apparatus and intersexual analia. Their brothers with one dose of endogenous dsx (row 8, Table 1) showed a significant increment in the size of the vaginal plates and a significant reduction in the size of the male genital arch, lateral plate and clasper structures, in addition to a large reduction in the size of the penis apparatus. In addition, the analia showed a greater degree of intersexuality. Hence, it appears that the transgenic Anastrepha DsxF protein can partially counteract the effect of the Drosophila DsxM protein, this effect being more intense the less of the latter protein there is.
The capability of the Anastrepha DsxM protein to compete with the endogenous Drosophila DsxF protein was analysed in sister XX flies expressing two MAo transgenes (MAo#10 and MAo#4) and carrying either two doses (dsx + / dsx + ) or one dose (dsx 1 / dsx + ) of the endogenous Drosophila dsx gene (cross V, Table 2). The XX flies with two doses of endogenous dsx showed some minor degree of intersexuality, manifested in their intersexual analia and the presence of a reduced penis apparatus always enclosed by the vaginal plates. Their brothers with one dose of endogenous dsx showed an increment in their intersexuality, observable by a reduction in the size of the vaginal plates along with an increase in the size of the penis apparatus, an increase in the intersexuality of the analia, and the presence of a male genital arch and lateral plate and clasper structures. Therefore, it appears that the transgenic Anastrepha DsxM protein is able to partially counteract the effect of the Drosophila DsxF protein, this effect being more intense the less of the latter protein there is.

Effect of the DsxF and DsxM proteins of Anastrepha on the regulation of Drosophila yolk protein genes
This is the new paragraph: The yolk protein (yp) genes of Drosophila are co-ordinately transcribed in the fat body of adult females under the control of dsx. DsxF and DsxM act as activator and repressor, respectively, by binding to the same regulatory sequences (reviewed in [29]). These genes are also expressed in the follicle cells of the ovary, although it appears that they are no longer under the control of dsx but are regulated by tissue specific factors present in those cells [30]. In loss-of-function dsx mutant XX flies lacking both DsxF and DsxM proteins, basal transcription of the yp genes in the fat body (gonads are not developed) has been reported [31], though in our dsx 1 stock such remnant expression was not observed.
The effect of Anastrepha DsxF protein on the regulation of Drosophila yp genes was studied my monitoring the expression of yp2 in transgenic Drosophila XX flies mutant for dsx and expressing the Anastrepha DsxF protein. The inducible HS-GAL4 driver was used to express the FAo#2 transgene. XX flies of genotype FAo#2/+; dsx 1 /+ (control females), FAo#2/+; dsx 1 /dsx 1 (intersexual flies) and FAo#2/HS-GAL4; dsx 1 /dsx 1 (experimental flies) were produced at 25uC (cross VI in Materials and Methods). After the eclosion of the adults, each class of females was divided into two populations; one was maintained at 25uC and the other subjected to heat-shock pulses to induce the expression of the transgene. All three classes of females received heat shock treatment at the same time (see legend to Fig. 4A). Total RNA was extracted and used in RT-PCR to determine the expression of yp2 and the expression of rp49 (which codes for the constitutive ribosomal protein 49) [32] (used as a control; for details see Materials and Methods). The results are presented in Figure 4. As expected, the control females expressed the yp2 gene whereas intersexual flies did not, whether kept at 25uC or subject to heat shock. Neither did the experimental females express the yp2 gene when maintained at 25uC, although they did express it after heat shock. The three classes of females expressed the control rp49 gene when kept at 25uC and after heat shock. These results indicate that the Anastrepha DsxF protein behaves as an activator of the Drosophila yp genes, just like DsxF of Drosophila.
To determine whether the Anastrepha DsxM protein acts as an inhibitor of the yp genes, as does that of Drosophila itself, normal, XX, fertile females of genotype MAo#10/+; dsx 1 /+ (control females) and MAo#10/HS-GAL4; dsx 1 /+ (experimental females) were raised at 25uC (cross VII in Materials and Methods). The same experimental plan described above was followed. Both control and experimental females produced the Drosophila endogenous DsxF protein, while the experimental females also produced the transgenic Anastrepha DsxM protein when subjected to the heat shock. As expected, the control females expressed the yp2 gene whether maintained at 25uC or when subjected to heat shock. The experimental females also expressed the yp2 gene when maintained at 25uC. These females, however, did not express this gene after heat shock, i.e., when the transgenic Anastrepha DsxM protein is produced (Fig. 4B). These results show that this protein counteracts the activation of the yp genes by the endogenous Drosophila DsxF protein.

Discussion
This investigation provided the following major results. 1) The Anastrepha DsxF and DsxM proteins cause feminisation and masculinisation respectively of dsx intersexual Drosophila flies, though this transformation is incomplete and their influence varies between the different sexually dimorphic structures. 2) The Anastrepha DsxF and DsxM proteins behave as activator and repressor respectively, of the Drosophila yolk protein genes, as do the DsxF and DsxM proteins of Drosophila itself. 3). The Anastrepha DsxF and DsxM proteins are able to counteract the function of Drosophila DsxM and DsxF respectively, just as these latter proteins behave towards one another. Collectively, these results indicate that the Anastrepha DsxF and DsxM proteins show conserved female and male sex-determination function respectively in Drosophila. Nevertheless, it appears that they cannot fully substitute the Drosophila Dsx proteins.
This incomplete function might be partly due to the insufficient amount of Anastrepha Dsx proteins produced in Drosophila transgenic flies, since the presence of two doses (rather than one) of AodsxF or AodsxM transgenes enhanced the corresponding modification towards female or male. It should be remembered that the GAL4/UAS system, the effectiveness of which depends on temperature, was used to express the Anastrepha dsx transgene, and that the flies had to be raised at 18 or 22uC to allow the transgenic Drosophila flies to reach adulthood and sexual transformation to be monitored. More complete sexual transformation might be obtained by allowing the production of greater amounts of Anastrepha Dsx proteins, but this could not be tested since the transgenic Drosophila died at higher temperatures.
There is, however, a clear-cut result; namely, the different developmental response of sexually dimorphic regions to the function of Anastrepha Dsx proteins. This cannot be explained by substantially different amounts of these proteins being present, since the GAL-4 driver is constitutively expressed in these structures. Rather, the present results suggest that the partial sex-determination function of the Anastrepha Dsx proteins in Drosophila reflect a reduced capacity of the Anastrepha Dsx proteins to completely control the Drosophila sexual cytodifferentiation genes in the different sexually dimorphic regions.
The development of sexually dimorphic structures not only depends on gene dsx but on an integrated signal involving the corresponding Dsx protein -either male or female -and the appropriate homeotic protein that determines segmental specificity. Thus, the sexual phenotype of the prothoracic leg basitarsus (i.e., either formation of the sex comb in males or its absence in females) requires, besides dsx, additional inputs from the homeotic gene Sex comb reduced (Scr) (which specifies prothoracic identity) and from the Distal-less gene (which specifies proximal-distal identity) [21]. The sexual phenotype of the abdominal tergites requires, besides dsx, inputs from the homeotic gene Abdominal-B [33,34]. Finally, besides dsx, the sexual development of the genital disc requires inputs from the homeotic genes Abdominal-B [35,36,37] and caudal [38,39]. Therefore, the dissimilar effect of the Anastrepha correspond to the offspring of crosses VI and VII respectively (see Materials and Methods). yp2 and rp49 stand for the yolk protein 2 and ribosomal protein 49 genes respectively. '25uC' indicates that the flies were maintained at this temperature after eclosion whereas HS indicates that they were subject to two 3 h heat shock pulses (37uC) per day for two consecutive days with recovery at 25uC between pulses. PCR amplification of total RNA extracts (without cDNA) yielded no amplification product, indicating that the RNA sample was devoid of DNA. doi:10.1371/journal.pone.0005141.g004 Dsx proteins on the development of different Drosophila sexually dimorphic regions may be a consequence of the accumulation of divergence between these species resulting in the formation of different co-adapted complexes between the Dsx proteins and the target genes.
In the above context, the following results are important. Firstly, the expression of Musca domestica DsxM protein in Drosophila XX flies does not affect normal female development except for variable male-like pigmentation in the 5th and 6th tergites in some flies [7]. In contrast, the expression of Ceratitis capitata DsxM protein in Drosophila females induces partial masculinisation [12], as does Anastrepha DsxM protein (this work). Ceratitis and Anastrepha belong to the family Tephritidae, whereas Musca belongs to Muscidae. The molecular evolution of gene dsx in insects shows that the Dsx proteins of the tephritids are more closely related to those of Drosophila than to those of Musca Dsx [14]. Together, these results suggest that the evolutionary divergence among Dsx proteins is greater between Musca and Drosophila than between Drosophila and the tephritids.
Secondly, the DsxF protein needs to interact with the Intersex protein in order to perform its function [40,41,42]. The Musca [7] and the Anastrepha (this work) DsxF protein can induce the synthesis of Drosophila yolk protein genes, indicating that they can interact with the Drosophila Intersex protein. It is thus suggested that the dissimilar function of the tephritid and Musca Dsx proteins in Drosophila might be due to different evolutionary changes in these proteins and/or the other regulatory proteins involved in the integrated signal dictating the developmental route the sexual dimorphic structures will follow. It should also be remembered that the D. melanogaster flies simultaneously expressing DsxF and DsxM proteins show both male and female genital structures and intersexual analia [24,25,26]. In interspecific hybrids expressing the Drosophila teissieri DsxF protein and the Drosophila melanogaster DsxM (teissieri-melanogaster hybrids), however, the external genitalia might be defined as more male-like than intersexual. Indeed, they have an almost completely normal set of male genital structures. Nonetheless, the analia remain intersexual [43]. To explain the male-like phenotype of the genitalia of these hybrids, the latter authors speculated that during the evolution of the D. melanogaster and D. teissieri species, genetic changes occurred in regulatory genes such as dsx and/or Abd-B, and/or in the genes controlled by these regulators, all of which are responsible for the development of the terminalia. These species-specific variations might be responsible for the morphological changes observed in the terminalia of these species. When the genotypes of the two species are put together within a hybrid cell, divergent co-adapted gene complexes confront one another. This might result in the formation of hybrid patterns different from those of either parental species; i.e., in the production of morphological diversity [43].
Finally, recent molecular data supports the formation of different co-adapted complexes between the Abd-B and Dsx proteins and its target genes. It has been found that Abd-B and dsx act in concert upon the cis-regulatory element (CRE) of the gene bric-à-brac (bab) to control the sexually dimorphic development of the 5th and 6th tergites in Drosophila melanogaster. In females, Abd-B and DsxF activate bab, whereas in males DsxM represses it, thus allowing for malespecific pigmentation. This genetic control evolved through changes within the CRE element of gene bab [34]. For a discussion of CREs changes in morphological evolution see Carroll [44].
In conclusion, it is proposed that the different sensitivity of the different sexually dimorphic regions of Drosophila to the Anastrepha Dsx proteins reflects the accumulation of different evolutionary changes not only in the Dsx proteins of these two species but also in their genes specifying segmental identity. As a result, the integrated genetic input determining the sexual development of each of these dimorphic regions is affected in different way.

Flies and crosses
Flies were cultured on standard food. For the description of the mutant alleles or GAL4 constructs see Lindsley and Zimm [45] and FlyBase. Flies used for the analysis of adult forelegs, abdomens and external terminalia were kept in a mixture of ethanol:glycerol (3:1) for several days. They were then macerated in 10% KOH at 60uC for 15 min, thoroughly washed with water and mounted in Faure's solution for inspection under a compound microscope. FAo and Mao stand for UAS::AodsxF-cDNA and UAS::AodsxM-cDNA, respectively. The crosses were: For the construction of the UAS::AodsxF-cDNA and UAS::AodsxM-cDNA transgenes, a fragment of 1568 bp, or 1579 bp, comprising the whole ORF of Anastrepha obliqua dsxF, or dsxM, was amplified by RT-PCR using a common primer at the 59UTR (59GTGAGT-CAGGGTTTAGCTC39) and a female-specific primer (59GTCATTGTTCCGCAAACATGG39) or a male-specific primer (59CAGTGAGTCAGGGCTTTAGC39) at the corresponding 39UTR. The amplicon was cloned in the TOPO-TA cloning vector (Invitrogen). The cDNA fragments were then digested with Eco RI and cloned in pUAST vector [20]. The microinjections for generating the FAo and MAo transgenic Drosophila melanogaster lines were performed by Genetic Services (Sudbury, MA, USA). Standard genetic crosses determined the chromosomal location of the transgenes. To ascertain that each transgenic line was carrying the correct transgene, PCR on genomic DNA was used to amplify the whole transgene and the amplicons were cloned and sequenced.

Molecular analyses
Total RNA extracts from frozen adults were prepared using the Ultraspec-II RNA isolation kit (Biotecx) following the manufacturer's instructions. Five micrograms of total RNA from each sample were reversed transcribed with Superscript II (Invitrogen) following the manufacturer's instructions. Reverse transcription reactions were performed with an oligo-dT. Two percent of the synthesised cDNA was amplified by PCR. The amplicons were analysed by electrophoresis in agarose gels. The primers used in the PCR for the analysis of the yp2 expression in the Drosophila transgenic flies were (59GTCGTTGAGGCCACCATGC39) and (59GGAGTGGTTCGCTCGCATG39), which amplify a fragment of 368 bp. As a control, the expression of gene rp49 [32] was monitored by PCR using the same cDNA sample used for the analysis of yp2. The PCR primers used for rp49 were (59ATCCGC-CACCAGTCGGATC39) and (59TGGCGCGCTCGACAATC-TC39), which amplify a fragment of 286 bp. Ten percent of the cDNA was used for PCR in a total volume of 50 ml. The PCR conditions were 95uC, 2 minutes, followed by 45 cycles of 95uC for 45 s, 59uC for 45 s, and 72uC for 1 min, plus an extension step at 72uC for 1 min. Fifteen microlitres of the yp2 PCR reaction volume and 8 ml of the rp49 PCR reaction volume were loaded onto gels for electrophoresis.