Antagonistic Bacterial Interactions Help Shape Host-Symbiont Dynamics within the Fungus-Growing Ant-Microbe Mutualism

Conflict within mutually beneficial associations is predicted to destabilize relationships, and theoretical and empirical work exploring this has provided significant insight into the dynamics of cooperative interactions. Within mutualistic associations, the expression and regulation of conflict is likely more complex than in intraspecific cooperative relationship, because of the potential presence of: i) multiple genotypes of microbial species associated with individual hosts, ii) multiple species of symbiotic lineages forming cooperative partner pairings, and iii) additional symbiont lineages. Here we explore complexity of conflict expression within the ancient and coevolved mutualistic association between attine ants, their fungal cultivar, and actinomycetous bacteria (Pseudonocardia). Specifically, we examine conflict between the ants and their Pseudonocardia symbionts maintained to derive antibiotics against parasitic microfungi (Escovopsis) infecting the ants' fungus garden. Symbiont assays pairing isolates of Pseudonocardia spp. associated with fungus-growing ants spanning the phylogenetic diversity of the mutualism revealed that antagonism between strains is common. In contrast, antagonism was substantially less common between more closely related bacteria associated with Acromyrmex leaf-cutting ants. In both experiments, the observed variation in antagonism across pairings was primarily due to the inhibitory capabilities and susceptibility of individual strains, but also the phylogenetic relationships between the ant host of the symbionts, as well as the pair-wise genetic distances between strains. The presence of antagonism throughout the phylogenetic diversity of Pseudonocardia symbionts indicates that these reactions likely have shaped the symbiosis from its origin. Antagonism is expected to prevent novel strains from invading colonies, enforcing single-strain rearing within individual ant colonies. While this may align ant-actinomycete interests in the bipartite association, the presence of single strains of Pseudonocardia within colonies may not be in the best interest of the ants, because increasing the diversity of bacteria, and thereby antibiotic diversity, would help the ant-fungus mutualism deal with the specialized parasites.


INTRODUCTION
Although mutually beneficial associations appear congenial, selection favoring individual partners using resources to facilitate their own selfish interests is predicted to destabilize cooperation [e.g. 1]; a paradox known as ''the tragedy of the commons'' [1][2]. This inherent conflict is recognized as shaping both within-and between-species cooperative relationships [3][4][5][6], and extensive theoretical and empirical studies examining conflict within intraspecific cooperative relationships have provided important insights into social evolution and the stability of cooperation [e.g., 3,[7][8]. The main mechanisms enforcing cooperation involve the alignment of interests of partners [e.g., kin selection theory: 7, [9][10] and sanctions against cheaters [e.g., worker policing, 3,8].
The regulation of conflict may be of particular importance within mutualistic associations, where partners are unrelated so cooperation cannot be explained by kin selection [7]. Mechanisms to reduce conflict, analogous to those within intraspecific cooperative relationships, appear to also govern interspecific interactions [6, 11; for a recent review, see 12]. Studies exploring conflict in mutualistic associations have documented alignment of interests through partner fidelity feedback, exemplified in joint mutualist transmission between host generations [vertical transmission; [13][14][15][16]. In addition, the presence of host sanctions directed towards cheating symbionts has been identified in several mutualisms [e.g., [17][18][19][20].
Although studies exploring mutualisms in a pair-wise framework have provided important insights into mutually beneficial interactions, the costs and benefits of cooperation and cheating within mutualisms can be significantly altered by the interactions that take place within the more complex communities in which they occur [e.g., [21][22]. For example, recent findings indicate that additional mutualistic and/or parasitic symbionts are often associated with mutualisms [e.g. 15, [23][24][25][26] and because these symbionts can alter the cost-benefit ratios within the focal bipartite association [27][28][29][30], they have the potential to shape the expression of conflict. Furthermore, populations of mutualists are composed of different symbiont genotypes, and conflict expression and regulation is likely to be influenced by host-mutualist genotype combinations as well as the avoidance of mixing of multiple genotypes within individual combinations [4][5][31][32]. In addition to populations being composed of multiple genotypes of Academic Editor: Leah Cowen, University of Toronto, Canada individual mutualistic species, a further level of complexity is the frequent presence of multiple species or strains of each mutualist lineage that can potentially form novel pairings of mutualists through host switches [cf. 16,[33][34][35]. Studies indicate that such novel pairings result in changes in the costs and benefits of the mutualism [e.g., [36][37], and, thus, likely influence the expression and regulation of conflict between cooperative partners. Evaluating the compatibility among symbiont species as well as the costs and benefits of different mutualistic species combinations across host-symbiont coevolutionary histories may inform our understanding of the role of conflict in shaping evolutionary dynamics of mutualisms, including host specificity, coevolutionary dynamics, and the stability of mutualism.
This study evaluates the expression of conflict within a broader symbiotic community context, as well as across symbiont lineages, using the fungus-growing ant-microbe symbiosis, a model system in mutualistic cooperation. This system provides a relatively unique opportunity for studying the complexity of symbiont conflict expression, because: i) it is a complex community involving at least three obligately associated mutualists and one specialized parasite [15,23]; ii) all four known symbionts are genetically and phylogenetically diverse, allowing for differences in cooperation and conflict between different genotype and species combinations to be investigated [34][35][36][38][39]; iii) all three attine-associated symbiotic microbes can be cultured, allowing for interactions within and between symbiont lineages to be examined [15,23,32,40]; iv) the coevolutionary histories of the symbiotic lineages have been explored [35,[41][42]Cafaro et al. in preparation]; and v) conflict is inherent and has been examined in the ant-cultivar association [32,34,36,38]. Here we employ symbiont pairing assay experiments, designed with particular emphasis on the factors described above, to evaluate the expression of conflict in the ant-Pseudonocardia association.

Cooperation and Conflict in the Fungus-Growing Ant-Microbe Symbiosis
Fungus-growing ants (Attini, Hymenoptera), a monophyletic group of more than 210 New World ant species, are characterized by their obligate dependence on fungi for food [41,43]. The ants cultivate their mutualistic fungus by providing it substrate for growth and protection from unfavorable abiotic and biotic conditions [15,23,28,[44][45][46][47]. Several mechanisms are present within the ant-fungus association that help reduce conflict and enforce cooperation. Vertical (uniparental) transmission of the fungal mutualist from parent to offspring nests by female alates helps align the reproductive interests of the ants and their fungi [5][6][13][14]34]. Although, as predicted by this transmission mode, fungal symbiont lineages have evolved in parallel with lineages of their ant hosts on the broad phylogenetic scale [e.g., 41], symbiont switching disrupts the expected pattern of strict lower-level phylogenetic ant-fungus congruence [36,42,[48][49]. The observation that host-symbiont switches are frequent, at least on an evolutionary time scale, indicates situations where the reproductive interests of the ants and fungi are decoupled and where mixing of genetically distinct fungus clones may incur competition [cf. 5-6; 32]. In the leaf-cutter ant Acromyrmex, competitive conflict between genetically distinct fungus clones is apparently prevented through behavioral recognition and removal of non-native fungi by the ants [36,50]. Furthermore, the resident fungus imprints ant faecal droplets, used to fertilize new mycelial growth, and this imprinting elicits the removal of compounds and fragments of non-native fungi, so that the residing fungus enforce single-clone rearing by precluding the establishment of additional clones within colonies [32].
The ants and their fungal cultivar are only two members of a more complex symbiotic community. The fungal mutualist is parasitized by specialized and coevolved microfungi in the genus Escovopsis (Ascomycota: Hypocreales) [23,35]. To help prevent and suppress Escovopsis infections the ants employ specific behaviors, including grooming their fungal mutualist and weeding out heavily infect garden fragments [47]. In addition, the ants engage in a second mutualism with actinomycetous bacteria (genus Pseudonocardia), which produce antibiotics capable of inhibiting the growth of the specialized parasite [15,28,45,51]. The antibioticproducing bacteria are typically housed in elaborate structures on the cuticle of workers, connected to exocrine glands that appear to provide nutrients to support the growth of Pseudonocardia [52]. The Escovopsis and Pseudonocardia symbionts are phylogenetically diverse and have an ancient and coevolved association with fungusgrowing ants and their fungal cultivar [35,[39][40][51][52]Cafaro et al. in preparation]. Individual ant colonies appear to associate with a single strain of the actinomycetous bacterium, but different ant species, and even genera, maintain very closely related Pseudonocardia symbionts [39]. Thus, even though the default vertical transmission mode predicts congruence between ant and bacterial phylogenies [15,45], horizontal exchanges of Pseudonocardia occur over evolutionary time and disrupt strict cocladogenesis (Cafaro et al. in preparation).
Although the ant-actinomycete mutualism is expected to be stabilized by the alignment of interests of specific symbiont pairs through vertical transmission and the rearing of single strains of Pseudonocardia within individual ant colonies [5][6]15,39], potential ant-bacterium conflict can be envisioned. Horizontal transfer of Pseudonocardia decouples the reproductive interests of the ants and the bacteria, and can result in the presence of more than a single genotype of Pseudonocardia within individual nests [cf. 39]. Further conflict is envisioned because Pseudonocardia produces antibiotics to help defend the ants' fungus garden from the horizontally transmitted Escovopsis parasite [15,23]. Observed variation in the inhibitory capabilities of different Pseudonocardia strains suggests that the actinomycetous symbionts are involved in Red-Queen-like antagonistic co-evolution with the parasite Escovopsis (Poulsen et al. in preparation), inferring that individual ant colonies would benefit from maintaining more than a single genotype of Pseudonocardia or from being able to easily acquire novel strains when confronted with resistant parasite strains. Replacement by, or mixing with, a foreign strain of Pseudonocardia would incur a direct cost to the resident Pseudonocardia strain, which would either be discarded or have to share both the energy allocated by the ants for growth and the transmission to the next generation on the cuticle of prospective ant queens [cf. [5][6]. A resident Pseudonocardia strain would therefore benefit from preventing foreign strains from invading its niche, and one likely mechanism to do so would be the production of compounds that inhibit other bacteria. Such antagonistic interactions are common in free-living bacteria [e.g., [53][54] by means of the production of bacteriocidal compounds usually targeted at conspecifics [bacteriocins; for a review, see 55].
In this study we examine the presence, frequency, and intensity of antagonistic interactions between pairs of Pseudonocardia symbionts originating from different fungus-growing ant colonies. We explore this through Petri dish bioassays pairing symbionts spanning the phylogeny of Pseudonocardia associated with fungusgrowing ants as well as free-living (non-ant associated) actinomycetes. In addition, we conduct a second set of bioassay challenges specifically focusing on a phylogenetically narrow group of strains isolated from sympatric and allopatric species of the leaf-cutting ant genus Acromyrmex. We evaluate the factors contributing to the observed patterns of antagonistic interactions, including the species or genus of ant host for the symbiotic Pseudonocardia strains. Additionally, we examine the relationship between antagonism and relative genetic difference between pairs of strains inferred from patterns of DNA sequence divergence analyses.

Experiments and bacterial isolates
We conducted two sets of bioassay experiments. The first examined interactions between strains of Pseudonocardia symbionts associated with fungus-growing ant species spanning most of the phylogenetic diversity of the ant-microbe mutualism (crossphylogeny bioassay experiment). Fourteen Pseudonocardia isolates were obtained from eight species of fungus-growing ants, including: Apterostigma dentigerum (belonging to one of the most phylogenetically basal ant genera), Cyphomyrmex costatus, C. muelleri, C. longiscapus (Cyphomyrmex being a phylogenetically basal/intermediate ant genus), Trachymyrmex zeteki, T. cornetzi (Trachymyrmex being a phylogenetically intermediate to derived non-leaf-cutting ant genus), Acromyrmex echinatior and A. octospinosus (Acromyrmex being one of the most derived attine ant genera (a leaf-cutter) [56]. Four free-living actinomycetes obtained from the USDA ARS culture collection (http://nrrl.ncaur.usda.gov; see Table 1) (Streptomyces griseus, Pseudonocardia saturnae, P. thermophilia, and P. halophobica), and a Nocardiodes sp., culture isolated from a colony of A. dentigerum, were also included in this experiment. The second bioassay experiment examined possible antagonism between bacterial symbionts associated with sympatric and allopatric species of the leaf-cutting ant genus Acromyrmex (within-Acromyrmex bioassay experiment). Specifically, we used 12 Pseudonocardia symbionts obtained from ant colonies from two geographically distant locations: Panama and Argentina. Eight strains originated from two sympatric Panamanian ant species (A. echinatior and A. octospinosus) collected from 2001-2005, and four strains were isolated from three ant species collected in Argentina in 2003 (one isolate from A. niger, two isolates from A. hispidus fallax, and one isolate from A. laticeps). The species, colony code, and geographic origin for each ant colony from which Pseudonocardia symbionts were isolated are provided in Table 1.

Actinomycete symbiont isolations
The Pseudonocardia symbionts used in this study were obtained using a modified method from Cafaro and Currie [51]. Actinomycete isolations were predominantly done on ants tending the fungus garden, since these workers carry the highest biomass of Pseudonocardia [28,57]. Since only one strain of bacterium is expected to be associated with a given ant colony [cf. 39], isolation attempts were done until a Pseudonocardia strain was obtained; this meant performing isolations on between one and ten ants per colony. In the ant genera Apterostigma and Cyphomyrmex, actinomycetes were isolated by placing individual ants, either whole or ground-up, in 750 ml autoclaved water in a 1.5 ml eppendorf tube. The tube was mixed for ,30 seconds using a vortex, and 100-250 ml of the suspension was subsequently spread onto chitin-agar plates containing antifungals (nystatin 10,000 units/ml and cycloheximide 5% w/v). To obtain Pseudonocardia symbionts from ants in the genera Trachymyrmex and Acromyrmex, bacteria were either isolated following the protocol describe above, or by aseptically scraping bacterial cells from the propleural plates with a scalpel [cf. 15,57]. After 2-3 weeks of growth at room temperature, Pseudonocardia colonies were transferred to Petri plates containing yeast malt extract agar (YMEA) with antifungals (concentrations as above) [cf. 51].

Bioassay challenges
In both experiments, biossay challenges involved co-culturing two strains of actinomycetes on YMEA with antifungals (concentrations as above) in 8.5 cm diameter Petri dishes. The first strain (hence forth referred to as the ''resident'' strain) was point inoculated in the center of the Petri dish and left to grow at room temperature for three weeks (Fig. 1). The second strain (hence forth referred to as the ''intruder'' strain) was inoculated to the entire unoccupied Petri dish area by applying 200 ml of autoclaved water containing a suspension of approximately 800-1200 bacterial cells per ml (Fig. 1). The plates were checked regularly until the intruder strain had sufficient time to grow to fill the Petri plate or a distinct zone of inhibition (zoi) imposed by the resident strain had established and no longer changed over time. When present, typically one week after the intruder strain was applied, the minimum zone of inhibition (zoi) imposed on the intruder strains were measured as well as the minimum diameter of the resident strain (Fig. 1). Actinomycete strains were paired in all possible combinations, 361 in the cross-phylogeny experiment and 144 in the within-Acromyrmex experiment, and three replicates were performed for each pairing. In the cross-phylogeny bioassay experiment the three replicates for each pairing were applied on three succeeding days, because the application of the second strain was labor intensive.

Genetic comparison of actinomycete strains
To infer phylogenetic relationships between Pseudonocardia strains used in the experiments, and to determine if genetic distance between strains influences antagonistic reactions, we did partial sequencing of ribosomal 16S rDNA and nuclear Elongation Factor-Tu (EF-Tu) regions using standard PCR and sequencing techniques [39,51,58]. PCR amplicons, generated using already published primers [39,51], were sequenced at the UW-Madison Biotechnology Center (http://www.biotech.wisc.edu). The resulting sequences were corrected for mismatches using Sequencher 4.6 for Windows (Gene Codes Corporation, Ann Arbor, MI), and aligned using Clustal X 1.83 [59] and MacClade 4.07 OS X [60]. Phylogenetic analyses were performed in PAUP* 4.0b 10 [61] and bootstrap support was assessed with 1000 pseudoreplicates under Maximum Parsimony (MP), Maximum Likelihood (ML), and Neighbor-Joining (NJ) conditions. Prior to performing the ML analyses, the models of sequence evolution were determined in Modeltest 3.7 [62]. Gaps in the sequences were treated as missing data.

Statistical analyses
Due to the non-normality of the data, and the high sensitivity of 0values in log-linear models, the statistical approach used to analyzing the bioassays was a generalized linear model design with an underlying gamma distribution. The exact values of the zoi measurements were inserted in the analyses, except that 0-values were imputed with 0.0001. All analyses were performed using SAS/STAT software, Version 9.1.3 of the SAS System for Microsoft Windows (Copyright ß2007 SAS Institute Inc. SAS and all other SAS Institute Inc. product or service names are registered trademarks of SAS Institute Inc., Cary, NC, USA), and significance of terms in the models was determined through Likelihood Ratio Statistics for Type III ANOVAs. Two models were built for each of the bioassay experiments. A nested model contained ant host of resident and intruder actinomycetes (genus in the cross-phylogeny bioassay, species in the within-Acromyrmex bioassay), resident and intruder actinomycete strains nested within their respective ant origins, and the interaction between ant hosts of the two strains. The minimum diameter of the resident actinomycete at the time the zoi of the intruder actinomycete was scored, and the relative genetic distance between pairs of actinomycetes were inserted as continuous covariates. Due to the nested design of the models, they could not include the interaction term between the resident and the intruder actinomycete strains. An additional model was therefore built for each bioassay, including only resident actinomycete

RESULTS
Our cross-phylogeny bioassay experiment, pairing strains of Pseudonocardia isolated from different colonies of attine ants spanning most of the diversity of the tribe and some non-ant associated free-living isolates, revealed a high proportion (208 out of 342) of pairings of actinomycetes with detectable levels of inhibition of the intruder strain by the resident strain (Fig. 2). Although antagonism was common, the ability to inhibit other bacteria varied significantly, with individual strains inhibiting between four (21.1%) and 17 (89.5%) of the intruder strains (on average 57.9%, 11 of 19; a significant effect of the resident strain origin in the nested model: Table 4, p,0.0001) (Fig. 2). As expected from the normal synchronization of the expression of genes coding for secondary metabolites and resistance in bacteria [e.g., 64], all Pseudonocardia strains were resistant to their own compounds (diagonal in Fig. 2). While all 19 intruder strains were susceptible to inhibition by at least six resident strains, they all exhibited resistance to compounds produced by some strains (ranging from 3-12 of pairings of actinomycetes from different colonies, average seven), resulting in a significant effect of the intruder strain (Table 4, nested model: p,0.0001) (Fig. 2). Thus, the outcome of a given pair-wise interaction was influenced by the combination of the strength of inhibition by the resident strain and the degree of intruder susceptibility (Table 4, statistical interaction term in actinomycete origin model: p,0.0001). The Mantel test of matrix correlation indicated asymmetry in pairs of strains; the same level of inhibition was not necessarily obtained when switching the order of inoculation of a given pair of strains (Mantel r = 20.09; p = 0.8562). Symbiont strains isolated from the  The frequency of base pair differences (relative genetic distance), averaged over partial sequences of the 16S and EF-Tu regions (1393 bp and 1004 bp, respectively), between actinomycete strains involved in the cross-phylogeny bioassay. Averaged, Isolates are listed according to ant species origin and the strains are numbered 1-19 (see Table 1  The frequency of base pair differences (pair-wise relative genetic distances), averaged over partial sequences of the 16S and EF-Tu regions (1394bp and 959bp, respectively), between actinomycete strains involved in the within-Acromyrmex bioassay. Colonies are labeled A-L according to  same ant genus displayed more similar inhibition and susceptibility profiles compared to strains isolated from different ant genera (Table 4, nested model: p,0.0001 and p = 0.005 for resident and intruder strain ant genus origin, respectively; Fig. 2B). The relative genetic distance between pairs of actinomycetes also had a significant effect on inhibition (Table 4, nested model: p = 0.0078), suggesting that antagonism is more common between more distantly related pairs of strains. The size of the resident strain did not affect the degree of antagonism (Table 4, nested model: p = 0.6892).
In the within-Acromyrmex bioassay experiment, antagonism was uncommon, with only 16 of the 132 pairings of actinomycetes originating from different colonies (12.1%) exhibiting inhibition (Fig. 3). There was a significant difference in the ability of Acromyrmex symbionts to inhibit other strains, with five strains showing no sign of antagonism, four strains inhibiting just a single intruder, and one strain inhibiting seven of the intruders (strains inhibited on average 1.3 intruders; Table 4, nested model: p,0.0001). There was also a significant difference in the strains resistance to inhibition: four of the intruders were not susceptible to inhibition by any of the resident strains and the remaining strains were inhibited by 1-5 strain(s) (on average two; Table 4, nested model: p,0.0001). Similar to the cross-phylogeny assay, the interaction between resident and intruder actinomycetes was even stronger than the isolated factors alone (Table 4, actinomycete origin model: p,0.0001). Furthermore, the degree of antagonism was affected by the host ant species for both the resident (p,0.0001) and intruder (p,0.0001) symbionts (Table 4, nested model; Fig. 3B). In addition, we found a significant effect of the relative genetic distance between pairs of strains, indicating a genetic effect on the degree of antagonism (Table 4, nested model: p = 0.0007; Fig. 3A). Finally, contrasting the cross-phylogeny bioassay, the size of the resident strain had a significant effect on the observed antagonism (Table 4, nested model: p = 0.0089). However, size of strain was no longer significant if the host ant species for the symbionts were not included in the model (Table 4, actinomycete origin model: p = 0.4735). As in the cross-phylogeny bioassay, the observed reactions were not symmetric (Mantel r = 20.07; p = 1.00), so that reactions were dependent upon the order of strains used in a given pairing.

DISCUSSION
Our bioassay experiments revealed the presence of antagonistic interactions among Pseudonocardia symbionts of fungus-growing ants. Conflict reactions between strains were observed in more than half the pairings of actinomycetes isolated from across the fungus-growing ant tribe, with most strains being capable of inhibit the growth of at least 20% of the actinomycetes they were tested against. The widespread ability of ant-associated Pseudonocardia to inhibit other symbiont strains suggest that competitive conflict will often be expressed when actinomycete strains mix and thereby may directly impact host-symbiont dynamics. Furthermore, the presence of reactions across the coevolutionary history of the antactinomycete association suggests that bacteria-bacteria antagonism has been present since the origin of the fungus-growing antmicrobe symbiosis.
Although our results indicate that Pseudonocardia bacteria have the capacity to inhibit other Pseudonocardia strains, there was abundant variation in both the ability to inhibit other strains and in the resistance to compounds. Inhibition is more likely between pairs of symbionts associated with more distantly related genera of fungus-growing ants, since antagonism was far more common in our cross-phylogeny experiment (57.9% of pairings revealed antagonism) as compared to pairings in the within-Acromyrmex Table 4. Statistical results of the generalized linear models with underlying gamma distributions for the two Petri dish bioassays. However, this effect may be at least partially due to the significant effect of genetic distance between pairs of strains ( Figs. 2A, 3A), because individual ant species or genera rear relatively closely related strains compared to the overall diversity of Pseudonocardia associated with fungus-growing ants. The mixing of more distantly related actinomycete strains, often associated with different species or genera of ants, could thus incur stronger conflict expression when mixes occur between strains across the broad attine ant groups than within these groups, potentially impacting dynamics of host-symbiont switching (see below). Although host ant genus and genetic distance between strains had a significant effect on antagonism observed between strains, they did not explain all the variation observed in our experiment. Across the diversity of Pseudonocardia symbionts, we found some strains that were highly potent (i.e., had the ability to inhibit a high proportion of the symbionts they were challenged with), as well as particularly resistant strains (i.e., with the ability to resist inhibition by a large proportion of resident strains). In both experiments the primary factors contributing to the variation in antagonism were i) the strength of inhibition by the residing bacterium, ii) the degree of susceptibility of the intruder, and iii) the combination of inhibitory capabilities and susceptibility (Figs. 2, 3; Table 4). This indicates that the outcome of interactions is strongly affected by the secreted secondary metabolites and the presence/absence of resistance to these secretions. This implies that strains with higher potency would be predicted to benefit from being able to resist replacement by a larger collection of actinomycetes, while strains able to resist inhibition would be predicted to be better at invading new colonies. Future work examining the costs associated with these phenotypic properties of different Pseudonocardia strains would provide important insights into the ant-bacteria mutualism.
The observed antagonistic reactions resemble interactions in free-living actinomycetes [53-54; this study], where they are believed to contribute significantly to microbial fitness in competition for nutrient and space resources [for a review, see 55]. Species of bacteria from all major lineages are known to secrete tens to hundreds of different biologically active proteins with a bacteriocidal mode of action (bacteriocins), which are believe to target mostly conspecifics [65-66 and references therein]. The observed antagonism between Pseudonocardia symbionts in this study is likely due to bacteriocins secreted to the surrounding medium. We suggest that selection on preventing the invasion and establishment of competing strains has resulted in the capacity of Pseudonocardia symbionts to uphold production of these compounds. Although it is possible that the observed antagonism is a result of the antibiotics produced by Pseudonocardia to inhibit the garden pathogen Escovopsis, this appears to be unlikely for several reasons. First, the antibiotics produced target the Escovopsis fungal parasites, and are thus expected to target the physiology of eukaryotes and not prokaryotes. Second, the pathogenic specificity of Escovopsis towards the ants' cultivar [35,40,[67][68] and the variability in potency of the Pseudonocardia-derived antibiotics against Escovopsis (Poulsen et al. in preparation) suggest that actinomycete-Escovopsis interactions are governed by dynamics of antagonistic coevolution. This predicts that antibiotics targeted at Escovopsis are subject to specialization over evolutionary time and that inhibition of other microorganisms in the association, such as the mutualistic fungus and the actinomycete bacteria, should be diminished. Third, in a separate study, some of the same Pseudonocardia strains were used in symbionts-pairing assays with Escovopsis to examine the specificity of activity of antifungal against diverse strains of the parasite (Poulsen et al. in preparation). When comparing individual strains in these two studies, there was no correspondence in the inhibition profiles against other strains of Pseudonocardia and Escovopsis (i.e., Pseudonocardia strains that inhibited a similar collection of other actinomycete symbionts in this study, did not correspondingly inhibit a similar collection of Escovopsis). Since the chemical composition of secretions from Pseudonocardia remains unknown, it is not currently possible to determine whether bacteriocins or antifungals are responsible for the actinomycete-actinomycete antagonism. However, even if antagonism is a bi-product of antibiotics targeting Escovopsis, activity against other strains of Pseudonocardia would still shape the dynamics of the association by precluding the rearing of bacterial strains exhibiting antagonistic interactions within individual ant colony.
The presence of antagonism infers that competitive conflict may occur between pairs of actinomycetes if multiple genotypes of actinomycete symbionts are present within an individual colony. Mixing likely occurs during events of horizontal transfers of Pseudonocardia, known to take place on both an ecological and an evolutionary time scale [39;Cafaro et al. in preparation]. As discussed above, the effect of ant host genus in the cross-phylogeny experiment, as well as the antagonistic interactions being significantly less common in the within-Acromyrmex experiment, supports relatively more potent reactions between actinomycetes originating from different host ant genera. This indicates that the potential for conflict is likely less pronounced if mixes occur between more closely related ants, while stronger reactions between actinomycetes reared by different ant genera could restrict switching of bacterial lineages across the attine tribe, contributing to the maintenance of host-mutualist cocladogenesis ( Fig. 2A; Cafaro et al. in preparation). While it is not in the interest of the actinomycetes to share ant-allocated resources, the display of antagonism indicates that events of mixing may incur a significant allocation of resources to competition rather than actinomycete growth and thereby also a potential reduction in the benefits of the association to the ants [cf. [5][6]. All available evidence indicates that individual colonies rear only a single actinomycete strain [39;Cafaro et al. in preparation], and considering the bipartite ant-actinomycete association (absence of parasitism), single-strain rearing would be in the interest of both ants and Pseudonocardia [cf. [5][6]39]. Behavioral tests support that Acromyrmex ants preferentially choose their resident actinomycete over very closely related non-native strains, indicating the presence of a mechanism that further facilitates avoidance of multiple competing actinomycete strains within colonies [58].
In the respective bipartite associations between attine ants and their fungal and bacterial mutualists, default vertical symbiont transmission aligns the reproductive interests of the partners [13][14][15] and single symbiont rearing within individual colonies prevents the expression of competitive symbiont-symbiont conflict [32,39]. Both ant-mutualist associations occasionally experience horizontal transmission, potentially decoupling the reproductive interests of host and symbionts and incurring competition between mixed symbiont strains. Furthermore, both symbionts have the ability to express antagonism that facilitates the maintenance of single symbionts within individual colonies. However, in the hostpathogen association between ants, cultivar, and actinomycete on one side and Escovopsis on the other, partner interests likely change. The rearing of a single fungal clone within a colony is not expected to pose a cost to the ants in relation to increased colony susceptibility to infection, because the cultivar itself does not have an efficient defense against typically infecting Escovopsis [68; Poulsen et al. in preparation; Little et al. in preparation]. However, the rearing of a single strain of actinomycetes could constrain the effectiveness of the ant-Pseudonocardia symbiosis, because multiple strains of antibiotic-producing bacteria within individual colonies would increase the diversity of compounds produced to defend against Escovopsis (Poulsen et al. in preparation). While single-strain rearing within individual colonies nevertheless is apparent, the extent to which horizontal switches of the bacterial mutualists take place appears to be symptomatic of the role they play in the association. Even though antactinomycete phylogenetic comparisons reveal some degree of host-mutualist cocladogenesis, actinomycete switches appear to take place more frequently than ant-fungus exchanges, including across the broad groupings governing ant-fungus associations ( Fig. 2A; Cafaro et al. in preparation). This suggests that while ant recognition of actinomycete symbionts may aid in the prevention of conflict between different strains, it may at the same time allow for the ants to actively pursue replacing their resident actinomycete strain to acquire novel antibiotics against Escovopsis.
Conflict is an inherent major force shaping mutualistic interactions [4][5][6]. Studies exploring conflict within the fungusgrowing ant-microbe symbiosis have identified the presence of behavioral ant-fungus incompatibilities and direct antagonism between fungal symbionts associated with different colonies, indicating that conflict shapes the mutualistic association [32,34,36,38]. Our findings indicate additional layers of complexity of conflict expression within the fungus-growing ant microbesymbiosis. This complexity is brought about through the widespread presence of antagonism between Pseudonocardia bacteria maintained by different ant colonies, species, and genera, but also by the presence of additional symbiont lineages (the Escovopsis parasites) that likely adjust the dynamics of cooperation and conflict within associations previously examined in a bipartite framework. Future studies exploring ant-bacteria and bacteriacultivar conflict will inevitably provide additional insights into the expression and regulation of conflict within complex mutualistic associations.