Representative TEM images of microtubules in axons.
Microtubules are indicated by black arrows (B, D). Axon diameter, number of microtubules, and spacing between microtubules were measured for each image along unit axon length, L, at L/4, L/2, and 3L/4. (A) In Control axons, microtubules are oriented along the principal axis of the axon. (C) In Crushed axons, microtubules appear disorganized and misaligned. (B, D) Inset of Control and Crushed axons showing diameter (D) and spacing (SMT) measurements for microtubules. (E) Nodal bleb (arrow head) of Crushed axon showing mitochondria in each bleb. (F) Inset of Crush axon showing microtubule breakage and rupture (arrows). Parts of fig are adapted from . Image used with permission from the Federation of American Societies for Experimental Biology or The FASEB Journal (www.fasebj.org). Scale bars = 100nm (A-D); 500nm (E-F).