Table 1.
Antibodies were diluted as follows.
Fig 1.
Detection of apoptosis, calcium, and ROS levels.
(a) Flow detection of apoptosis levels in each group. (b) The mRNA expression of DHHC6 was detected by RT-qPCR. (c) Fluo-4 AM fluorescent probe for calcium levels in each group. (d) ROS fluorescent probe to detect ROS level. *p < 0.05，**p < 0.01，***p < 0.001.
Fig 2.
Detection of cellular mitochondrial membrane potential and γ-H2AX expression levels.
(a) Mitochondrial membrane potential of each group of cells was detected by the JC-1 kit. (b) Statistical analysis of mitochondrial membrane epotential of each group of cells detected by the JC-1 kit. (c) Statistical analysis of immunofluorescence staining for detecting the expression level of γ-H2AX. (d) Immunofluorescence staining to detect the expression level of γ-H2AX. *p < 0.05，**p < 0.01，***p < 0.001.
Fig 3.
Detection of cellular aging indicators.
(a) Western Blot was used to detect RB, RB2, p107, p53, p27, p21, ARF, and p16 to evaluate the details of each group. Changes in cellular aging. (b) Observation of cellular aging through Ki-67 staining. (c) Observation of cellular aging by β-galactosidase staining. *p < 0.05，**p < 0.01，***p < 0.001.
Fig 4.
Western Blot detection of Bax, Bcl-2, cleaved Caspase8, cleaved Caspase3, Col2, AGG, MMP3, MMP13 protein expression levels.
***p < 0.001.
Fig 5.
Detection of protein expression levels and observation of cellular microstructure.
(a) Percoll centrifugation to isolate subcellular structural MAMs, and Western Blot to detect GRP75, VDAC1, Drp1, and Mfn2 protein expression levels. ***p < 0.001. (b) TEM observation of cell microscopic morphology.
Fig 6.
Detection of IP3R S-palmitoylation modification and protein expression levels.
(a) ABE and Co-IP detect IP3R S-palmitoylation modification levels. (b) Western Blot detection of IP3R protein expression level. ***p < 0.001.