Fig 1.
Correlation of IPSS with Qmax, of IPSS with prostate volume, and of Qmax with prostate volume in the examined patient population (n = 146 patients).
Laser-enucleated samples from 100 patients were subsequently analyzed by RT-PCR, and 46 samples by Western blot analyses. Data for prostate volume was available from 145 patients, data for IPSS from 87 patients, and for Qmax from 47 patients. Correlation analyses were performed by Pearson correlation analyses.
Fig 2.
Detection of calponin and cytokeratin expression in laser-enucleated prostate tissues.
Laser-enucleated prostate tissues were subjected to detection of calponin-1 (CNN1) and keratin-19 (KRT19) mRNA expression by RT-PCR (a, b), and of calponin and pan-cytokeratin protein expression by Western blot analyses (c). Shown are all single values from RT-PCR in (a), which were compared between catheterized (n = 38) and non-catheterized patients (n = 61) in (b) (CNN1 compared by unpaired, two-tailed t-test; KRT19 and CNN1/KRT19 compared by Mann Whitney test; all insignificant). Representative Western blots are shown in (c). All three blots are from the same sample set. Numbers on the left indicate positions of molecular weight markers. Data for prostate volume, IPSS and Qmax from the corresponding patients are indicated on the top (n.a., information not available).
Fig 3.
Expression of calponin-1 and keratin-19 mRNA in laser-enucleated prostate tissues, and correlation with IPSS, Qmax and prostate volume. Laser-enucleated tissues (n = 100 patients) were subjected to RT-PCR for calponin-1 (CNN1) and keratin-19 (KRT19).
The ratio of 2-ΔCt values for CNN1 and KRT19 (CNN1/KRT19) (a), 2-ΔCt values for CNN1 (b), and 2-ΔCt values for KRT19 (c) were subjected to Pearson correlation analyses with prostate volume, IPSS and Qmax in these patients.
Fig 4.
Correlation of CNN1 and KRT19 mRNA expression with prostate volume in patients with and without catheterization.
Laser-enucleated tissues (n = 100) were subjected to RT-PCR for calponin-1 (CNN1) and keratin-19 (KRT19). Separate Pearson correlation analyses for mRNA expression with prostate volume were performed for patients without catheterization (a) and with catheterization for urinary retention (b).
Fig 5.
Protein expression of calponin and cytokeratins in laser-enucleated prostate tissues, and correlation with IPSS, Qmax and prostate volume. Laser-enucleated tissues (n = 46 patients) were subjected to Western blot analyses for cytokeratins, calponin and β-actin.
Intensities of bands from 37-50 kDa in pan-cytokeratin expression (a), and matching the expected molecular weight of calponin (33 kDa) (b) were referred to band intensities of β-actin in the same samples, and subjected to Pearson correlation analyses with prostate volume, IPSS and Qmax in these patients.