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Table 1.

Sequence of the primers used in this study.

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Table 2.

Measurement of growth plate and analysis of growth zones.

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Table 3.

Comparison of growth plate aromatase activity between obese and control.

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Table 4.

Comparison of serum insulin levels between obese and control.

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Fig 1.

Histology of chondrocytes (A) based on their growth plates and zones (B) control group and (C) obese group. Magnification 200x.

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Fig 2.

Immunohistochemistry results of aromatase (a.control group,b.obese group) and insulin receptor (c. control group,d.obese group) in SD rats. Magnification of 200x (scale – 50μm).

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Table 5.

The results of qPCR for CYP19A1 and IR in two groups.

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Table 6.

The results of Western blot analysis for CYP19A1 and IR in t two groups.

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Fig 3.

(A) Amplication and melt curves (B) Relative mRNA expression of IR and CYP19A1.

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Fig 4.

(A) electrophoresis results (B) quantification of IR and CYP19A1.

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