Fig 1.
Pie charts representing the numbers of sequences available for A. each species B. each subtilisin subtypes in the dataset used in the study.
Fig 2.
Probable correct identification values for all of the Arthrodermataceae genera from trimmed dataset.
A. The PCI values were estimated for all subtilisin subtypes for each species based on sequence similarity. B. Comparison between subtilisin subtypes (SUB1-SUB7). Significant difference between the subtypes is represented as * (p ≤ .05), ** (p ≤ .01), *** (p ≤ .001). SUB2 and SUB5 are eliminated since the PCI score is <10%.
Fig 3.
Box plot plotted for intraspecies and interspecies distance to demonstrate the subtilisin gene overlap for a given species.
TB: T.benhamiae; TT: T.tonsurans; TE: T.equinum; TR: T.rubrum; TM: TS: T.soudanense; T.mentagrophytes; TI: T.interdigitale; MC: M.canis; TV: T.verrucosum; EF: E.floccosum. A: SUB1; B: SUB3; C: SUB4; D: SUB6; E: SUB7.
Fig 4.
PCR targeting subtilisin region.
M:100 base pair marker; 1: Trichophyton interdigitale; 2: T. mentagrophytes; 3: T. rubrum, 4: T. tonsurans; 5: M. canis; 6: N. gypsea; 7: E. floccosum; 8: Rhizopus stolonifer.; 9: Aspergillus niger; 10: A. flavus; 11: Penicillium citrinum.; 12–17: Trichophyton Clinical isolates.
Fig 5.
Phylogenetic tree for diversity of species based on subtilisin region obtained in the study: Eight isolates identified as T. mentagrophytes clustered with standard T. mentagrophytes reference sequence, 26 isolates clustered as T. mentagrophyte complex.
Three isolates clustered with T. tonsurans reference sequence. The three isolates identified as N. gypsea and one isolate identified as Epidermophyton floccosum clustered around reference sequences of M. canis and N. gypsea. Sequences marked * indicate that these sequences were taken from NCBI nucleotide. All other sequences were generated in this study.
Table 1.
Dermatophyte subtilisin specific primer, sequences and annealing temperatures.
Table 2.
Accession numbers obtained for NCBI submissions.