Fig 1.
Representative stromal localization of Chondroitin Sulfate in G1-EEC.
Back-to-back glandular crowding (A) and papillary architecture (C). CS (red arrows) showed reactivity surrounding the glandular lumen (green asterisk) and the fine fibrovascular stroma supporting the papillary architecture (B, D). Hematoxylin and eosin (H&E) staining results (A, C). CS-immunostaining (B, D). Original magnification: A–D, 100×.
Fig 2.
Representative stromal localization of CS in a cribriform pattern.
Infiltrating fused tumor gland forming a glandular lumen without intervening stroma (A). CS localization reflects this architecture, and CS-immunostaining is poorly reactive around the glandular lumen (green asterisks) (B). H&E staining (A); CS-immunostaining (B). Original magnification: A–B, 100×.
Fig 3.
Representative stromal localization of CS in a solid growth pattern.
In solid growth patterns such as G3-EEC (A, D), CS (B, E: red arrows) exhibited dot- or liner-like expression patterns in concordance with endothelial cells that expressed the CD31 marker (C, F: green arrows). H&E staining (A, D). CS-immunostaining (B, E). CD31-immunostaining (C.F). Original magnification: A–C, 40×; C–E, 100×.
Table 1.
CS-immunostaining scores in the peri glandular stromal region.
Fig 4.
Representative stromal localization of CS in ESC.
This tumor type showed a predominant papillary architecture with irregular papillae (A, C). The thick fibrous strands showed immunoreactivity via CS-immunostaining (red arrows) but poor reactivity in the elongated, branched, and delicate stromal regions (blue frame; B, D). H&E staining (A, C). CS-immunostaining (B, D). Original magnification: A–B, 40×; C–D, 100×.
Fig 5.
Typical AB staining patterns in EC.
Representative immunohistochemical localization of CS in G1-EEC (A) and ESC (C). AB pH 1.0 staining showed similar reactivity with CS-immunostaining results (B, D). CS-immunostaining (A, C). AB staining (B, D). Original magnification: A–D, 100×.