Fig 1.
Schematic illustration of experimental design.
Presumptive zygotes were cultured individually and embryonic development monitored with images captured by the Primo Vision TL system for a period of 7.5 days. At day 9.5 post IVF, blastocysts were classified according to their post-hatching development (V: Viable, NV: Non-viable).
Fig 2.
Individual culture to monitor embryonic development (Primovision®, Vitrolife, Sweden).
Table 1.
Production of bovine embryos in vitro in a time-lapse system and culture in drops (laboratory control).
Table 2.
Characteristics of in vitro cultured blastocyst according to in vitro viability determined by extended culture up to 9.5 days post IVF.
Table 3.
Characteristics of blastocyst cultured in vitro according to blastulation time.
Fig 3.
Bovine embryonic development in microwell culture system according to viability (Viable “green line” and non-viable (red line).
A) Morphokinetic parameters of embryonic divisions t2, t3, t4, t5, t8, t9+, tM, tSB, tB and tBX; B) Time of cell cycle and synchrony t3-t2, t4-t3, t5-t4, t8-t5, t9+-t8, tM-t9+, tSB-ttM, tB-tSB, tBX-tB. (*) It indicates that in this morphokinetic parameter there is a statistical difference (P<0.05); Scatter plot illustrating two variables from the morphokinetics data, where colour represents in vitro viability, C) t2 (h) division time to 2 cells; D) Embryo diameter at tSB; and E) Embryo diameter at day 7.5 post IVF, with tSB (h) time of starting blastulation, respectively.
Fig 4.
Pearson correlation coefficient and principal component analysis for all variables with in vitro viability.
A) Variables con Pearson’s correlation coefficient significative (p<0.01). PCA analysis of morphokinetics variables (B) and morphokinetics variables and embryo diameter after the start of blastulation (C) according to their in vitro viability (V = viable and N = non-viable).
Table 4.
Non-invasive predictive models of in vitro viability using binary logistic regression.
Table 5.
Logistic regression parameters of model-3 to predict viability in vitro.