Table 1.
Insecticidal activity of spore-crystal mixtures derived from Bacillus thuringiensis subspecies kurstaki HD-1 and Bacillus thuringiensis subspecies thuringiensis HD-2 towards first instar larvae of Agrotis exclamationis, scored seven days after treatment.
Table 2.
Insecticidal activity of Bacillus thuringiensis proteins towards first instar larvae of Agrotis exclamationis, based upon mortality scored seven days after treatment.
Fig 1.
Weight of Agrotis exclamationis surviving larvae exposed to Bacillus thuringiensis Cry and Vip protoxins in relation to control.
Bars and whiskers indicate means ± SD of two replicate trials. Three concentrations used for each protein are indicated in the figure legend. Significant differences in weight between treated larvae and the control, based on t-test (p<0.05), are indicated with asterisks.
Fig 2.
Histopathological effects of Bacillus thuringiensis insecticidal proteins on Agrotis exclamationis larval midgut.
The effects, visible under transmission electron microscope, were captured after 24 h (±4) exposition of larvae to: (a, b) untreated control; (c, d) 1000 ng/cm2 of Cry9Ea protoxin; (e, f) 1000 ng/cm2 of Vip3Aa protoxin. Av–autophagic vacuole; M–mitochondrion; Mv–microvilli; N–nucleus; R–ribosomes; V–vacuole.