Fig 1.
Particle yield and purity analysis of exosomes.
Interleaved scatter bar graphic presents the descriptive data of (A) descriptive data of protein concentration (mg/ml) (B) particle number and (C) purity ratio analysis of MACS and ultracentrifugation groups. ** indicates p<0.05. (●) presented each individual measurement value.
Fig 2.
Flow cytometric analysis of exosomes.
CD9 and CD81 surface expression on exosomes isolated by (A) MACS and (B) ultracentrifugation were analyzed by flow cytometry. (C) The MFI ratios of isotype control, ultracentrifuged, and MACS isolated- exosomes are shown. *** indicates p<0.001.
Fig 3.
Transmission electron micrographs of exosomes.
Negatively stained spherical structures in a range of sizes between 80–150 nm and 90–170 nm at the (A) ultracentrifugation (scale bar:100nm) and (B) MACS (scale bar:200nm, inset 50nm) groups, respectively. Exosomes are marked with white circles and exosome clusters are pointed by arrows. Descriptive Statistics (C) of exosome sizes are shown.